id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-300436-beb8k075	Zhang, Shuai	Transferrin receptor 1 is a supplementary receptor that assists transmissible gastroenteritis virus entry into porcine intestinal epithelium	2018-10-20		.txt	text/plain	5701	368	54	Because TfR1 is distributed widely along the surface epithelium of newborns with anemia, and intestinal epithelial cells of newborn piglets are targets of TGEV, it is possible that TfR1 is the as yet uncharacterized 200 kDa protein that mediates TGEV infection. To test this hypothesis, we used cultured porcine intestinal columnar epithelial cells (IPEC-J2), derived from the neonatal piglet mid-jejunum [39, 40] , as a model to characterize the interaction between TfR1 and TGEV in vitro. At 24 h p.i., western blotting showed that expression of TGEV-N protein was significantly lower in IPEC-J2 cells that had been pre-incubated with anti-TfR1 Ab (Fig. 3b and c) . c and d TGEV (MOI 5) was pre-incubated with the precipitated TfR1 protein for 2 h at 37°C before cells were infected. e and f IPEC-J2 cells infected with TGEV (MOI 5) were pre-incubated with TfR1-Out (200 ng/mL) for 1 h at 37°C, and cell lysates were harvested for western blotting.	./cache/cord-300436-beb8k075.txt	./txt/cord-300436-beb8k075.txt