id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302401-oyhzn2kc	Li, Chenxi	Duck karyopherin α4 (duKPNA4) is involved in type I interferon expression and the antiviral response against Japanese encephalitis virus	2019-11-05		.txt	text/plain	5192	332	52	Here, we cloned the duck KPNA4 (duKPNA4) gene and analyzed its involvement in type I IFN expression as well as antiviral response against Japanese encephalitis virus (JEV). We therefore cloned the duKPNA4 gene and analyzed its role in type I IFN expression as well as the antiviral response against JEV in primary duck embryo fibroblasts (DEFs). To analyze whether duKPNA4 might be involved in type I IFN expression, we transfected DEFs with plasmid for expression of duKPNA4-HA at different doses in the presence and absence of poly(I:C) stimulation, and detected the expression of interferon-α (IFN-α) and interferon-β (IFN-β) at the mRNA level by qRT-PCR and at the protein level by ELISA. Together, these data indicated that duKPNA4 resulted in nuclear translocation of duIRF7 and facilitated type I IFN expression during JEV infection, thereby implying its involvement in the type I IFN-mediated antiviral response.	./cache/cord-302401-oyhzn2kc.txt	./txt/cord-302401-oyhzn2kc.txt