id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-303186-2hxlx1j2	Won, Hokeun	Generation and protective efficacy of a cold-adapted attenuated genotype 2b porcine epidemic diarrhea virus	2019-07-09		.txt	text/plain	8115	320	45	In this study, we generated a cold-adapted live attenuated vaccine candidate (Aram-P29-CA) by short-term passage of a virulent PEDV isolate at successively lower temperatures in Vero cells. In this study, we sought to create a cold-adapted attenuated G2b PEDV low-passage strain by progressively decreasing growth temperatures to 32°C in Vero cells and then attempted to evaluate its protective efficacy on neonatal piglets against virulent PEDV challenge. Overall, the quantities of viruses in the feces of animals of group 2 significantly declined compared to those in group 1, with wide Ct ranges of 34.46-24.34 ( Efficacy of cold-adapted attenuated PEDV vaccine All animals in the parental Aram-P5-infected group were necropsied upon death at 4 or 5 DPI, while piglets in the remaining groups were euthanized at the end of the study for postmortem examinations (Fig. 7) .	./cache/cord-303186-2hxlx1j2.txt	./txt/cord-303186-2hxlx1j2.txt