id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-309565-8syjr6k8	KANNO, Toru	A long-term animal experiment indicating persistent infection of bovine coronavirus in cattle	2018-05-18		.txt	text/plain	2487	129	56	A long-term animal experiment involving inoculation with bovine coronavirus (BCoV) was conducted to verify its persistent infection in cattle. Until the end of the experiment (1,085, 700 and 280 days, respectively), viral RNAs were detected sporadically by RT-PCR and nested PCR from plasma, nasal discharge, and feces. Samples of nasal discharge, feces, plasma, and sera were collected daily until 10 days post inoculation (dpi), followed by weekly collection until 141 dpi and then twice-weekly collection until the end of the experiment (1,085 dpi). The virus in the digestive tract might have been quickly inactivated and excreted; therefore, viral RNAs were not detected from nasal discharge and feces at 421 dpi, 10 days after the onset. This study showed that the BCoV RNA was long-lasting, having been detected from the nasal discharge of cattle that had been maintained in an isolated room of a high-containment facility to prevent virus intrusion from outside.	./cache/cord-309565-8syjr6k8.txt	./txt/cord-309565-8syjr6k8.txt