id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-317389-trvleobp	Hoy, Carlton F.O.	Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection	2019-10-14		.txt	text/plain	4212	214	49	title: Rapid multiplex microfiber-based immunoassay for anti-MERS-CoV antibody detection The bulk of this robust antibody immunoassay platform could be installed into a compact syringe-driven cassette device, which could perform multiplex antibody immunoassay for antibodies specifically against Middle East respiratory syndrome coronavirus (MERS-CoV) with rapid preparation amounting to a total of 5 min, as well as high sensitivity and specificity for the MERS-CoV down to 200 μg/mL. In addition to the previously established fluorescently-linked immunosorbent assay (FLISA) microfiber platform [28, 37] , the microfiber mats were pre-patterned with O 2 plasma to create multiple hydrophilic zones with different antigens, and thus creating a multiplex detection system. Rapid immunoassay testing using the cassette platform were performed in following steps: (1) antigen immobilization, (2) blocking, and (3) antibody capture. The 8-layered 10 wt% ESPS fiber mat samples were treated by O 2 plasma spot treatment for different time lengths, then tested for protein adsorption to the surface.	./cache/cord-317389-trvleobp.txt	./txt/cord-317389-trvleobp.txt