id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-320559-up1q3k6q	Dortmans, J.C.F.M.	Porcine epidemic diarrhea virus (PEDV) introduction into a naive Dutch pig population in 2014	2018-05-24		.txt	text/plain	4216	207	57	Porcine epidemic diarrhea virus (PEDV) is the highly contagious, causative agent of an economically important acute enteric disease in pigs of all ages. In total, 838 blood samples from sows from 267 farms and 101 samples from wild boars were collected from May till November 2014 and tested for antibodies against PEDV by ELISA. The number of required blood samples from animals and farms to estimate the seroprevalence of PEDV in Dutch sow herds was calculated based on the following assumptions: PEDV is highly contagious and no vaccination against this virus was carried out in the Netherlands. For the detection of PEDV antibodies in serum samples an in-house indirect ELISA based on the viral spike (S) protein S1-part of the G2b strain GDU (Non-S-INDEL, GenBank KU985230.1) was used, similar as the ELISA previously described (Gerber et al., 2014) .	./cache/cord-320559-up1q3k6q.txt	./txt/cord-320559-up1q3k6q.txt