id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325679-4lfpy84d	Niu, Ting-Jiang	Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China	2018-12-06		.txt	text/plain	4831	227	56	To investigate the presence and genetic variability of FeKoV in northeast China, 197 fecal samples were collected from 105 cats with obvious diarrhea and 92 asymptomatic cats in Shenyang, Jinzhou, Changchun, Jilin and Harbin regions, Northeast China, and viruses were detected by RT-PCR with universal primers targeting all kobuviruses. By genetic analysis based on partial 3D gene, all kobuvirus-positive samples were more closely related to previous FeKoV strains with high identities of 90.5%–97.8% and 96.6%–100% at the nucleotide and amino acid levels. Additionally, phylogenetic analysis based on the complete VP1 gene indicated that all FeKoV strains identified in this study were placed into a cluster, which separated from other reference strains previously reported, and three identical amino acid substitutions were present at the C-terminal of the VP1 protein for these FeKoV strains. The genetic analysis based on the partial RdRp gene indicated that FeKoV strains shared higher nucleotide (81.2%-82.1%) and amino acid identities (91.4%-92.1%) with CaKoV strains previously reported (Kapoor et al.	./cache/cord-325679-4lfpy84d.txt	./txt/cord-325679-4lfpy84d.txt