id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-328000-i9tzr13z	Cockrell, Adam S.	Modeling pathogenesis of emergent and pre-emergent human coronaviruses in mice	2018-07-24		.txt	text/plain	11004	519	44	Three different strategies were employed for development of SARS-CoV mouse models: (i) different mouse species (or subspecies) were challenged with wildtype human SARS-CoV isolates in order to find a model that allows for replication and reflects severe respiratory disease symptoms observed in infected human patients; (ii) mice were genetically engineered to modify the host receptor, which facilitated productive SARS-CoV replication and pathogenesis; and (iii) adaptive evolution of wild-type SARS-CoV to a chosen mouse species was done to enhance pathogenesis, and associated clinical phenotypes in vivo. To adapt SARS-CoV to cause severe acute respiratory disease in mouse lungs, 6-week-old female BALB/c mice were intra-nasally infected with the clinical Urbani isolate (Roberts et al. Virus infection studies in CC mouse lines, including SARS-CoV, have led to mapping of high and low host response alleles as they relate to development of clinical signs of disease following viral pathogenesis (Bottomly et al.	./cache/cord-328000-i9tzr13z.txt	./txt/cord-328000-i9tzr13z.txt