id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-329760-mcfxye4f	Chung, Woo-Chang	Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate	2019-11-25		.txt	text/plain	4294	202	44	Using this sequence information, we constructed a CHIKV-pseudotyped lenti-virus expressing the structural polyprotein of the Korean CHIKV isolate (CHIKVpseudo) and dual reporter genes of green fluorescence protein and luciferase. Validation and comparisons of PBNA results with those from conventional PRNT indicate that the PBNA using CHIKVpseudo may be a safe, rapid, and reliable neutralization assay for evaluating the neutralizing activity of anti-sera against CHIKV. To produce CHIKVpseudo, HEK293T cells were transfected with pCMV2-FLAG-CHIKVst, psPAX2 (a gift from Dr. Seungmin Hwang, The University of Chicago, IL, USA), and Luc2P pLVX-IRES-ZsGreen1, which is able to express two reporter genes, luciferase and GFP (a gift from Dr. Nam-Hyuk Cho, Seoul National University, South Korea). Here, we adopted the strategy of expressing the entire structural polyprotein of CHIKV to generate the pseudotyped virus form of KNIH/2009/77 (CHIKVpseudo) for the enhanced infectivity compared to a virion expressing either an individual E1 or E2 protein (Hu et al., 2014) .	./cache/cord-329760-mcfxye4f.txt	./txt/cord-329760-mcfxye4f.txt