id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-331641-u27ohm5p	Liu, Xiaonan	A direct isothermal amplification system adapted for rapid SNP genotyping of multifarious sample types	2018-09-15		.txt	text/plain	3850	186	51	In this study, we devised a Direct-LAMP procedure, amplifying nucleic acids with various samples (including whole blood, dried blood spot, buccal swab and saliva) without DNA purification, which is essential for conventional nucleic acid detection methods. To evaluate the performance of the Direct-LAMP, a serial dilution of the target concentrations of whole blood sample and saliva sample with two different genotypes (wild type and homozygous mutation which confirmed by sequencing) of MTHFR C677T and ALDH2 Glu504Lys, respectively, were used to determine the detection limit. Here, we have established a rapid, easy-to-use and accurate SNP detection platform using Direct-LAMP, which enables us to use whole blood, dried blood spot, buccal swab or saliva as samples for genotyping without DNA purification. In this study, we presented a Direct-LAMP for SNP detection by using whole blood, dried blood spot, buccal swab or saliva as samples without DNA purification.	./cache/cord-331641-u27ohm5p.txt	./txt/cord-331641-u27ohm5p.txt