id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-349042-u9svz7pf	Li, Jifen	The successes and future prospects of the linear antisense RNA amplification methodology	2018-03-29		.txt	text/plain	5015	253	42	The technique was originally developed to assess RNA populations from small amounts of starting material, including single cells, but over time its use has evolved to include the detection of various cellular entities such as proteins, RNA-binding-protein-associated cargoes, and genomic DNA. The technique was originally developed to assess RNA populations from small amounts of starting material, including single cells, but over time its use has evolved to include the detection of various cellular entities such as proteins, RNA-binding-protein-associated cargoes, and genomic DNA. Examination of expression profiles of single live cells has shown that linear aRNA amplification neither results in occurs after synthesis of double-stranded cDNA, when T7 RNA polymerase is added and aRNA is transcribed from the cDNA template. 45 developed a method to facilitate aRNA detection of antibody-antigen interactions by covalently attaching a double-stranded cDNA that contains a T7 RNA polymerase promoter in front of a reporter sequence to a specific antibody.	./cache/cord-349042-u9svz7pf.txt	./txt/cord-349042-u9svz7pf.txt