id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350389-6o9t2am7	Guo, Xiao	Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats	2018-02-23		.txt	text/plain	8644	412	56	RESULTS: We used sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing electrophoresis (IEF), nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS) after in gel digestion of the proteins and quantitative real-time PCR (qRT-PCR) and showed that the levels of two MUPs, odorant-binding protein 3 (OBP3) (i.e. PGCL4) and MUP13 (i.e. PGCL1), in urine and their mRNAs in liver were higher in males than in females and were suppressed by orchidectomy and restored by testosterone treatment (T treatment). In the current study, we analysed MUPs in Lewis rats using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), quantitative real-time PCR (qRT-PCR), isoelectric focusing electrophoresis (IEF), In gel digestion and nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS), and then generated rMUPs. We further examined the activity of rMUPs by testing whether these proteins elicited behavioural and neuronal responses in female rats.	./cache/cord-350389-6o9t2am7.txt	./txt/cord-350389-6o9t2am7.txt