id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-356094-sbtigcfr	Chen, Huijie	Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.	2019-10-29		.txt	text/plain	8921	485	55	perforatum was ethyl acetate extraction section (HPE), and results showed that treatment with HPE significantly reduced the relative messenger ribonucleic acid (mRNA) expression and virus titer of IBV, and reduced positive green immunofluorescence signal of IBV in chicken embryo kidney (CEK) cells. The results of adaptation and replication in CEK cells, such as CPE, reverse transcription polymerase chain reaction (RT-PCR), and IBV growth curve determined by tissue culture infective dose (TCID 50 ) at different time were tested. The relative mRNA expression level of IBV-N gene was detected by qRT-PCR and the virus titer of IBV was determined by TCID 50 to analyze the antiviral effect of HPE, HPW and SEE (Figure 3) . FIgUre 8 | The effects of Hypericum perforatum ethyl acetate (HPE) on infectious bronchitis virus (IBV) messenger ribonucleic acid (mRNA) expression levels of trachea (A) and kidney (B).	./cache/cord-356094-sbtigcfr.txt	./txt/cord-356094-sbtigcfr.txt