id sid tid token lemma pos cord-349042-u9svz7pf 1 1 key key JJ cord-349042-u9svz7pf 2 1 : : : cord-349042-u9svz7pf 2 2 cord-349042-u9svz7pf cord-349042-u9svz7pf NN cord-349042-u9svz7pf 2 3 authors author NNS cord-349042-u9svz7pf 2 4 : : : cord-349042-u9svz7pf 2 5 Li Li NNP cord-349042-u9svz7pf 2 6 , , , cord-349042-u9svz7pf 2 7 Jifen Jifen NNP cord-349042-u9svz7pf 2 8 ; ; : cord-349042-u9svz7pf 2 9 Eberwine Eberwine NNP cord-349042-u9svz7pf 2 10 , , , cord-349042-u9svz7pf 2 11 James James NNP cord-349042-u9svz7pf 2 12 title title NN cord-349042-u9svz7pf 2 13 : : : cord-349042-u9svz7pf 3 1 The the DT cord-349042-u9svz7pf 3 2 successes success NNS cord-349042-u9svz7pf 3 3 and and CC cord-349042-u9svz7pf 3 4 future future JJ cord-349042-u9svz7pf 3 5 prospects prospect NNS cord-349042-u9svz7pf 3 6 of of IN cord-349042-u9svz7pf 3 7 the the DT cord-349042-u9svz7pf 3 8 linear linear JJ cord-349042-u9svz7pf 3 9 antisense antisense NN cord-349042-u9svz7pf 3 10 RNA RNA NNP cord-349042-u9svz7pf 3 11 amplification amplification NN cord-349042-u9svz7pf 3 12 methodology methodology NN cord-349042-u9svz7pf 3 13 date date NN cord-349042-u9svz7pf 3 14 : : : cord-349042-u9svz7pf 3 15 2018 2018 CD cord-349042-u9svz7pf 3 16 - - HYPH cord-349042-u9svz7pf 3 17 03 03 CD cord-349042-u9svz7pf 3 18 - - HYPH cord-349042-u9svz7pf 3 19 29 29 CD cord-349042-u9svz7pf 3 20 journal journal NN cord-349042-u9svz7pf 3 21 : : : cord-349042-u9svz7pf 4 1 Nat Nat NNP cord-349042-u9svz7pf 4 2 Protoc Protoc NNP cord-349042-u9svz7pf 4 3 DOI DOI NNP cord-349042-u9svz7pf 4 4 : : : cord-349042-u9svz7pf 5 1 10.1038/ 10.1038/ LS cord-349042-u9svz7pf 6 1 nprot.2018.011 nprot.2018.011 NNP cord-349042-u9svz7pf 6 2 sha sha NNP cord-349042-u9svz7pf 6 3 : : : cord-349042-u9svz7pf 7 1 5d902f20842783b98928a4c7147fb9da89a01825 5d902f20842783b98928a4c7147fb9da89a01825 NNP cord-349042-u9svz7pf 7 2 doc_id doc_id CD cord-349042-u9svz7pf 7 3 : : : cord-349042-u9svz7pf 7 4 349042 349042 CD cord-349042-u9svz7pf 7 5 cord_uid cord_uid NNS cord-349042-u9svz7pf 7 6 : : : cord-349042-u9svz7pf 7 7 u9svz7pf u9svz7pf NNP cord-349042-u9svz7pf 8 1 It -PRON- PRP cord-349042-u9svz7pf 8 2 has have VBZ cord-349042-u9svz7pf 8 3 been be VBN cord-349042-u9svz7pf 8 4 over over IN cord-349042-u9svz7pf 8 5 a a DT cord-349042-u9svz7pf 8 6 quarter quarter NN cord-349042-u9svz7pf 8 7 of of IN cord-349042-u9svz7pf 8 8 a a DT cord-349042-u9svz7pf 8 9 century century NN cord-349042-u9svz7pf 8 10 since since IN cord-349042-u9svz7pf 8 11 the the DT cord-349042-u9svz7pf 8 12 introduction introduction NN cord-349042-u9svz7pf 8 13 of of IN cord-349042-u9svz7pf 8 14 the the DT cord-349042-u9svz7pf 8 15 linear linear NNP cord-349042-u9svz7pf 8 16 RNA RNA NNP cord-349042-u9svz7pf 8 17 amplification amplification NN cord-349042-u9svz7pf 8 18 methodology methodology NN cord-349042-u9svz7pf 8 19 known know VBN cord-349042-u9svz7pf 8 20 as as IN cord-349042-u9svz7pf 8 21 antisense antisense NNP cord-349042-u9svz7pf 8 22 RNA RNA NNP cord-349042-u9svz7pf 8 23 ( ( -LRB- cord-349042-u9svz7pf 8 24 aRNA arna NN cord-349042-u9svz7pf 8 25 ) ) -RRB- cord-349042-u9svz7pf 8 26 amplification amplification NN cord-349042-u9svz7pf 8 27 . . . cord-349042-u9svz7pf 9 1 Whereas whereas IN cord-349042-u9svz7pf 9 2 most most JJS cord-349042-u9svz7pf 9 3 molecular molecular JJ cord-349042-u9svz7pf 9 4 biology biology NN cord-349042-u9svz7pf 9 5 techniques technique NNS cord-349042-u9svz7pf 9 6 are be VBP cord-349042-u9svz7pf 9 7 rapidly rapidly RB cord-349042-u9svz7pf 9 8 replaced replace VBN cord-349042-u9svz7pf 9 9 owing owe VBG cord-349042-u9svz7pf 9 10 to to IN cord-349042-u9svz7pf 9 11 the the DT cord-349042-u9svz7pf 9 12 fast fast RB cord-349042-u9svz7pf 9 13 - - HYPH cord-349042-u9svz7pf 9 14 moving move VBG cord-349042-u9svz7pf 9 15 nature nature NN cord-349042-u9svz7pf 9 16 of of IN cord-349042-u9svz7pf 9 17 development development NN cord-349042-u9svz7pf 9 18 in in IN cord-349042-u9svz7pf 9 19 the the DT cord-349042-u9svz7pf 9 20 field field NN cord-349042-u9svz7pf 9 21 , , , cord-349042-u9svz7pf 9 22 the the DT cord-349042-u9svz7pf 9 23 aRNA arna NN cord-349042-u9svz7pf 9 24 procedure procedure NN cord-349042-u9svz7pf 9 25 has have VBZ cord-349042-u9svz7pf 9 26 become become VBN cord-349042-u9svz7pf 9 27 a a DT cord-349042-u9svz7pf 9 28 base base NN cord-349042-u9svz7pf 9 29 that that WDT cord-349042-u9svz7pf 9 30 can can MD cord-349042-u9svz7pf 9 31 be be VB cord-349042-u9svz7pf 9 32 built build VBN cord-349042-u9svz7pf 9 33 upon upon IN cord-349042-u9svz7pf 9 34 through through IN cord-349042-u9svz7pf 9 35 varied varied JJ cord-349042-u9svz7pf 9 36 uses use NNS cord-349042-u9svz7pf 9 37 of of IN cord-349042-u9svz7pf 9 38 the the DT cord-349042-u9svz7pf 9 39 technology technology NN cord-349042-u9svz7pf 9 40 . . . cord-349042-u9svz7pf 10 1 The the DT cord-349042-u9svz7pf 10 2 technique technique NN cord-349042-u9svz7pf 10 3 was be VBD cord-349042-u9svz7pf 10 4 originally originally RB cord-349042-u9svz7pf 10 5 developed develop VBN cord-349042-u9svz7pf 10 6 to to TO cord-349042-u9svz7pf 10 7 assess assess VB cord-349042-u9svz7pf 10 8 RNA RNA NNP cord-349042-u9svz7pf 10 9 populations population NNS cord-349042-u9svz7pf 10 10 from from IN cord-349042-u9svz7pf 10 11 small small JJ cord-349042-u9svz7pf 10 12 amounts amount NNS cord-349042-u9svz7pf 10 13 of of IN cord-349042-u9svz7pf 10 14 starting start VBG cord-349042-u9svz7pf 10 15 material material NN cord-349042-u9svz7pf 10 16 , , , cord-349042-u9svz7pf 10 17 including include VBG cord-349042-u9svz7pf 10 18 single single JJ cord-349042-u9svz7pf 10 19 cells cell NNS cord-349042-u9svz7pf 10 20 , , , cord-349042-u9svz7pf 10 21 but but CC cord-349042-u9svz7pf 10 22 over over IN cord-349042-u9svz7pf 10 23 time time NN cord-349042-u9svz7pf 10 24 its -PRON- PRP$ cord-349042-u9svz7pf 10 25 use use NN cord-349042-u9svz7pf 10 26 has have VBZ cord-349042-u9svz7pf 10 27 evolved evolve VBN cord-349042-u9svz7pf 10 28 to to TO cord-349042-u9svz7pf 10 29 include include VB cord-349042-u9svz7pf 10 30 the the DT cord-349042-u9svz7pf 10 31 detection detection NN cord-349042-u9svz7pf 10 32 of of IN cord-349042-u9svz7pf 10 33 various various JJ cord-349042-u9svz7pf 10 34 cellular cellular JJ cord-349042-u9svz7pf 10 35 entities entity NNS cord-349042-u9svz7pf 10 36 such such JJ cord-349042-u9svz7pf 10 37 as as IN cord-349042-u9svz7pf 10 38 proteins protein NNS cord-349042-u9svz7pf 10 39 , , , cord-349042-u9svz7pf 10 40 RNA rna NN cord-349042-u9svz7pf 10 41 - - HYPH cord-349042-u9svz7pf 10 42 binding bind VBG cord-349042-u9svz7pf 10 43 - - HYPH cord-349042-u9svz7pf 10 44 protein protein NN cord-349042-u9svz7pf 10 45 - - HYPH cord-349042-u9svz7pf 10 46 associated associate VBN cord-349042-u9svz7pf 10 47 cargoes cargo NNS cord-349042-u9svz7pf 10 48 , , , cord-349042-u9svz7pf 10 49 and and CC cord-349042-u9svz7pf 10 50 genomic genomic JJ cord-349042-u9svz7pf 10 51 DNA dna NN cord-349042-u9svz7pf 10 52 . . . cord-349042-u9svz7pf 11 1 In in IN cord-349042-u9svz7pf 11 2 this this DT cord-349042-u9svz7pf 11 3 Perspective perspective NN cord-349042-u9svz7pf 11 4 we -PRON- PRP cord-349042-u9svz7pf 11 5 detail detail VBP cord-349042-u9svz7pf 11 6 the the DT cord-349042-u9svz7pf 11 7 linear linear JJ cord-349042-u9svz7pf 11 8 aRNA aRNA HYPH cord-349042-u9svz7pf 11 9 amplification amplification NN cord-349042-u9svz7pf 11 10 procedure procedure NN cord-349042-u9svz7pf 11 11 and and CC cord-349042-u9svz7pf 11 12 its -PRON- PRP$ cord-349042-u9svz7pf 11 13 use use NN cord-349042-u9svz7pf 11 14 in in IN cord-349042-u9svz7pf 11 15 assessing assess VBG cord-349042-u9svz7pf 11 16 various various JJ cord-349042-u9svz7pf 11 17 components component NNS cord-349042-u9svz7pf 11 18 of of IN cord-349042-u9svz7pf 11 19 a a DT cord-349042-u9svz7pf 11 20 cell cell NN cord-349042-u9svz7pf 11 21 's 's POS cord-349042-u9svz7pf 11 22 chemical chemical JJ cord-349042-u9svz7pf 11 23 phenotype phenotype NN cord-349042-u9svz7pf 11 24 . . . cord-349042-u9svz7pf 12 1 This this DT cord-349042-u9svz7pf 12 2 procedure procedure NN cord-349042-u9svz7pf 12 3 is be VBZ cord-349042-u9svz7pf 12 4 particularly particularly RB cord-349042-u9svz7pf 12 5 useful useful JJ cord-349042-u9svz7pf 12 6 in in IN cord-349042-u9svz7pf 12 7 efforts effort NNS cord-349042-u9svz7pf 12 8 to to TO cord-349042-u9svz7pf 12 9 multiplex multiplex VB cord-349042-u9svz7pf 12 10 the the DT cord-349042-u9svz7pf 12 11 simultaneous simultaneous JJ cord-349042-u9svz7pf 12 12 detection detection NN cord-349042-u9svz7pf 12 13 of of IN cord-349042-u9svz7pf 12 14 various various JJ cord-349042-u9svz7pf 12 15 cellular cellular JJ cord-349042-u9svz7pf 12 16 processes process NNS cord-349042-u9svz7pf 12 17 . . . cord-349042-u9svz7pf 13 1 These these DT cord-349042-u9svz7pf 13 2 efforts effort NNS cord-349042-u9svz7pf 13 3 are be VBP cord-349042-u9svz7pf 13 4 necessary necessary JJ cord-349042-u9svz7pf 13 5 to to TO cord-349042-u9svz7pf 13 6 identify identify VB cord-349042-u9svz7pf 13 7 the the DT cord-349042-u9svz7pf 13 8 quantitative quantitative JJ cord-349042-u9svz7pf 13 9 chemical chemical NN cord-349042-u9svz7pf 13 10 phenotype phenotype NN cord-349042-u9svz7pf 13 11 of of IN cord-349042-u9svz7pf 13 12 cells cell NNS cord-349042-u9svz7pf 13 13 that that WDT cord-349042-u9svz7pf 13 14 underlies underlie VBZ cord-349042-u9svz7pf 13 15 cellular cellular JJ cord-349042-u9svz7pf 13 16 function function NN cord-349042-u9svz7pf 13 17 . . . cord-349042-u9svz7pf 14 1 in in IN cord-349042-u9svz7pf 14 2 the the DT cord-349042-u9svz7pf 14 3 original original JJ cord-349042-u9svz7pf 14 4 cell cell NN cord-349042-u9svz7pf 14 5 or or CC cord-349042-u9svz7pf 14 6 tissue tissue NN cord-349042-u9svz7pf 14 7 are be VBP cord-349042-u9svz7pf 14 8 maintained maintain VBN cord-349042-u9svz7pf 14 9 . . . cord-349042-u9svz7pf 15 1 The the DT cord-349042-u9svz7pf 15 2 assumption assumption NN cord-349042-u9svz7pf 15 3 is be VBZ cord-349042-u9svz7pf 15 4 that that IN cord-349042-u9svz7pf 15 5 the the DT cord-349042-u9svz7pf 15 6 relative relative JJ cord-349042-u9svz7pf 15 7 abundances abundance NNS cord-349042-u9svz7pf 15 8 of of IN cord-349042-u9svz7pf 15 9 the the DT cord-349042-u9svz7pf 15 10 amplified amplified JJ cord-349042-u9svz7pf 15 11 RNAs rna NNS cord-349042-u9svz7pf 15 12 will will MD cord-349042-u9svz7pf 15 13 reflect reflect VB cord-349042-u9svz7pf 15 14 the the DT cord-349042-u9svz7pf 15 15 biological biological JJ cord-349042-u9svz7pf 15 16 state state NN cord-349042-u9svz7pf 15 17 of of IN cord-349042-u9svz7pf 15 18 the the DT cord-349042-u9svz7pf 15 19 source source NN cord-349042-u9svz7pf 15 20 , , , cord-349042-u9svz7pf 15 21 just just RB cord-349042-u9svz7pf 15 22 as as IN cord-349042-u9svz7pf 15 23 the the DT cord-349042-u9svz7pf 15 24 endogenous endogenous JJ cord-349042-u9svz7pf 15 25 RNA RNA NNP cord-349042-u9svz7pf 15 26 abundances abundance NNS cord-349042-u9svz7pf 15 27 do do VBP cord-349042-u9svz7pf 15 28 . . . cord-349042-u9svz7pf 16 1 To to TO cord-349042-u9svz7pf 16 2 avoid avoid VB cord-349042-u9svz7pf 16 3 systematic systematic JJ cord-349042-u9svz7pf 16 4 errors error NNS cord-349042-u9svz7pf 16 5 of of IN cord-349042-u9svz7pf 16 6 uneven uneven JJ cord-349042-u9svz7pf 16 7 amplification amplification NN cord-349042-u9svz7pf 16 8 of of IN cord-349042-u9svz7pf 16 9 different different JJ cord-349042-u9svz7pf 16 10 RNAs rna NNS cord-349042-u9svz7pf 16 11 that that WDT cord-349042-u9svz7pf 16 12 can can MD cord-349042-u9svz7pf 16 13 occur occur VB cord-349042-u9svz7pf 16 14 with with IN cord-349042-u9svz7pf 16 15 exponential exponential JJ cord-349042-u9svz7pf 16 16 amplification amplification NN cord-349042-u9svz7pf 16 17 procedures procedure NNS cord-349042-u9svz7pf 16 18 ( ( -LRB- cord-349042-u9svz7pf 16 19 where where WRB cord-349042-u9svz7pf 16 20 error error NN cord-349042-u9svz7pf 16 21 is be VBZ cord-349042-u9svz7pf 16 22 also also RB cord-349042-u9svz7pf 16 23 exponentially exponentially RB cord-349042-u9svz7pf 16 24 amplified amplify VBN cord-349042-u9svz7pf 16 25 ) ) -RRB- cord-349042-u9svz7pf 16 26 , , , cord-349042-u9svz7pf 16 27 scientists scientist NNS cord-349042-u9svz7pf 16 28 developed develop VBD cord-349042-u9svz7pf 16 29 linear linear JJ cord-349042-u9svz7pf 16 30 nucleic nucleic JJ cord-349042-u9svz7pf 16 31 acid acid NN cord-349042-u9svz7pf 16 32 amplification amplification NN cord-349042-u9svz7pf 16 33 . . . cord-349042-u9svz7pf 17 1 Linear Linear NNP cord-349042-u9svz7pf 17 2 RNA RNA NNP cord-349042-u9svz7pf 17 3 amplification amplification NN cord-349042-u9svz7pf 17 4 is be VBZ cord-349042-u9svz7pf 17 5 an an DT cord-349042-u9svz7pf 17 6 isothermal isothermal JJ cord-349042-u9svz7pf 17 7 nucleic nucleic JJ cord-349042-u9svz7pf 17 8 acid acid NN cord-349042-u9svz7pf 17 9 amplification amplification NN cord-349042-u9svz7pf 17 10 system system NN cord-349042-u9svz7pf 17 11 that that WDT cord-349042-u9svz7pf 17 12 rapidly rapidly RB cord-349042-u9svz7pf 17 13 amplifies amplify VBZ cord-349042-u9svz7pf 17 14 target target NN cord-349042-u9svz7pf 17 15 RNA RNA NNP cord-349042-u9svz7pf 17 16 populations population NNS cord-349042-u9svz7pf 17 17 . . . cord-349042-u9svz7pf 18 1 There there EX cord-349042-u9svz7pf 18 2 are be VBP cord-349042-u9svz7pf 18 3 two two CD cord-349042-u9svz7pf 18 4 primary primary JJ cord-349042-u9svz7pf 18 5 linear linear JJ cord-349042-u9svz7pf 18 6 amplification amplification NN cord-349042-u9svz7pf 18 7 procedures procedure NNS cord-349042-u9svz7pf 18 8 in in IN cord-349042-u9svz7pf 18 9 use use NN cord-349042-u9svz7pf 18 10 today today NN cord-349042-u9svz7pf 18 11 : : : cord-349042-u9svz7pf 18 12 linear linear JJ cord-349042-u9svz7pf 18 13 aRNA arna NN cord-349042-u9svz7pf 18 14 amplification amplification NN cord-349042-u9svz7pf 18 15 and and CC cord-349042-u9svz7pf 18 16 rolling roll VBG cord-349042-u9svz7pf 18 17 circle circle NN cord-349042-u9svz7pf 18 18 amplification amplification NN cord-349042-u9svz7pf 18 19 . . . cord-349042-u9svz7pf 19 1 In in IN cord-349042-u9svz7pf 19 2 this this DT cord-349042-u9svz7pf 19 3 Perspective perspective NN cord-349042-u9svz7pf 19 4 we -PRON- PRP cord-349042-u9svz7pf 19 5 concentrate concentrate VBP cord-349042-u9svz7pf 19 6 on on IN cord-349042-u9svz7pf 19 7 how how WRB cord-349042-u9svz7pf 19 8 the the DT cord-349042-u9svz7pf 19 9 aRNA arna NN cord-349042-u9svz7pf 19 10 amplification amplification NN cord-349042-u9svz7pf 19 11 methodology methodology NN cord-349042-u9svz7pf 19 12 has have VBZ cord-349042-u9svz7pf 19 13 been be VBN cord-349042-u9svz7pf 19 14 developed develop VBN cord-349042-u9svz7pf 19 15 for for IN cord-349042-u9svz7pf 19 16 a a DT cord-349042-u9svz7pf 19 17 host host NN cord-349042-u9svz7pf 19 18 of of IN cord-349042-u9svz7pf 19 19 chemical chemical JJ cord-349042-u9svz7pf 19 20 analyses analysis NNS cord-349042-u9svz7pf 19 21 , , , cord-349042-u9svz7pf 19 22 so so CC cord-349042-u9svz7pf 19 23 no no DT cord-349042-u9svz7pf 19 24 further further JJ cord-349042-u9svz7pf 19 25 discussion discussion NN cord-349042-u9svz7pf 19 26 of of IN cord-349042-u9svz7pf 19 27 rolling roll VBG cord-349042-u9svz7pf 19 28 circle circle NN cord-349042-u9svz7pf 19 29 amplification amplification NN cord-349042-u9svz7pf 19 30 ensues ensue VBZ cord-349042-u9svz7pf 19 31 . . . cord-349042-u9svz7pf 20 1 Linear linear JJ cord-349042-u9svz7pf 20 2 aRNA arna NN cord-349042-u9svz7pf 20 3 amplification amplification NN cord-349042-u9svz7pf 20 4 is be VBZ cord-349042-u9svz7pf 20 5 in in IN cord-349042-u9svz7pf 20 6 vitro vitro FW cord-349042-u9svz7pf 20 7 RNA rna NN cord-349042-u9svz7pf 20 8 - - HYPH cord-349042-u9svz7pf 20 9 transcription transcription NN cord-349042-u9svz7pf 20 10 - - HYPH cord-349042-u9svz7pf 20 11 mediated mediate VBN cord-349042-u9svz7pf 20 12 amplification amplification NN cord-349042-u9svz7pf 20 13 , , , cord-349042-u9svz7pf 20 14 and and CC cord-349042-u9svz7pf 20 15 is be VBZ cord-349042-u9svz7pf 20 16 also also RB cord-349042-u9svz7pf 20 17 referred refer VBN cord-349042-u9svz7pf 20 18 to to IN cord-349042-u9svz7pf 20 19 as as IN cord-349042-u9svz7pf 20 20 amplified amplify VBN cord-349042-u9svz7pf 20 21 RNA RNA NNP cord-349042-u9svz7pf 20 22 or or CC cord-349042-u9svz7pf 20 23 simply simply RB cord-349042-u9svz7pf 20 24 aRNA arna CD cord-349042-u9svz7pf 20 25 . . . cord-349042-u9svz7pf 21 1 This this DT cord-349042-u9svz7pf 21 2 technique technique NN cord-349042-u9svz7pf 21 3 was be VBD cord-349042-u9svz7pf 21 4 first first RB cord-349042-u9svz7pf 21 5 developed develop VBN cord-349042-u9svz7pf 21 6 and and CC cord-349042-u9svz7pf 21 7 published publish VBN cord-349042-u9svz7pf 21 8 in in IN cord-349042-u9svz7pf 21 9 1990 1990 CD cord-349042-u9svz7pf 21 10 ( ( -LRB- cord-349042-u9svz7pf 21 11 ref ref NN cord-349042-u9svz7pf 21 12 . . . cord-349042-u9svz7pf 22 1 2 2 LS cord-349042-u9svz7pf 22 2 ) ) -RRB- cord-349042-u9svz7pf 22 3 as as IN cord-349042-u9svz7pf 22 4 a a DT cord-349042-u9svz7pf 22 5 method method NN cord-349042-u9svz7pf 22 6 to to TO cord-349042-u9svz7pf 22 7 amplify amplify VB cord-349042-u9svz7pf 22 8 RNA RNA NNP cord-349042-u9svz7pf 22 9 populations population NNS cord-349042-u9svz7pf 22 10 , , , cord-349042-u9svz7pf 22 11 but but CC cord-349042-u9svz7pf 22 12 since since IN cord-349042-u9svz7pf 22 13 then then RB cord-349042-u9svz7pf 22 14 it -PRON- PRP cord-349042-u9svz7pf 22 15 has have VBZ cord-349042-u9svz7pf 22 16 been be VBN cord-349042-u9svz7pf 22 17 used use VBN cord-349042-u9svz7pf 22 18 for for IN cord-349042-u9svz7pf 22 19 a a DT cord-349042-u9svz7pf 22 20 number number NN cord-349042-u9svz7pf 22 21 of of IN cord-349042-u9svz7pf 22 22 other other JJ cord-349042-u9svz7pf 22 23 applications application NNS cord-349042-u9svz7pf 22 24 . . . cord-349042-u9svz7pf 23 1 In in IN cord-349042-u9svz7pf 23 2 this this DT cord-349042-u9svz7pf 23 3 procedure procedure NN cord-349042-u9svz7pf 23 4 , , , cord-349042-u9svz7pf 23 5 Van Van NNP cord-349042-u9svz7pf 23 6 Gelder Gelder NNP cord-349042-u9svz7pf 23 7 et et NNP cord-349042-u9svz7pf 23 8 al al NNP cord-349042-u9svz7pf 23 9 . . . cord-349042-u9svz7pf 24 1 2 2 LS cord-349042-u9svz7pf 24 2 first first RB cord-349042-u9svz7pf 24 3 used use VBN cord-349042-u9svz7pf 24 4 reverse reverse JJ cord-349042-u9svz7pf 24 5 transcription transcription NN cord-349042-u9svz7pf 24 6 to to TO cord-349042-u9svz7pf 24 7 create create VB cord-349042-u9svz7pf 24 8 a a DT cord-349042-u9svz7pf 24 9 cDNA cdna NN cord-349042-u9svz7pf 24 10 copy copy NN cord-349042-u9svz7pf 24 11 of of IN cord-349042-u9svz7pf 24 12 the the DT cord-349042-u9svz7pf 24 13 RNA RNA NNP cord-349042-u9svz7pf 24 14 template template NN cord-349042-u9svz7pf 24 15 , , , cord-349042-u9svz7pf 24 16 incorporating incorporate VBG cord-349042-u9svz7pf 24 17 an an DT cord-349042-u9svz7pf 24 18 RNA RNA NNP cord-349042-u9svz7pf 24 19 polymerase polymerase NN cord-349042-u9svz7pf 24 20 promoter promoter NN cord-349042-u9svz7pf 24 21 into into IN cord-349042-u9svz7pf 24 22 each each DT cord-349042-u9svz7pf 24 23 cDNA cdna NN cord-349042-u9svz7pf 24 24 molecule molecule NN cord-349042-u9svz7pf 24 25 by by IN cord-349042-u9svz7pf 24 26 priming prime VBG cord-349042-u9svz7pf 24 27 cDNA cdna NN cord-349042-u9svz7pf 24 28 synthesis synthesis NN cord-349042-u9svz7pf 24 29 with with IN cord-349042-u9svz7pf 24 30 a a DT cord-349042-u9svz7pf 24 31 synthetic synthetic JJ cord-349042-u9svz7pf 24 32 oligonucleotide oligonucleotide NN cord-349042-u9svz7pf 24 33 ( ( -LRB- cord-349042-u9svz7pf 24 34 oligo(dT)-T7 oligo(dt)-t7 NN cord-349042-u9svz7pf 24 35 ) ) -RRB- cord-349042-u9svz7pf 24 36 that that WDT cord-349042-u9svz7pf 24 37 contained contain VBD cord-349042-u9svz7pf 24 38 a a DT cord-349042-u9svz7pf 24 39 poly(T poly(t JJ cord-349042-u9svz7pf 24 40 ) ) -RRB- cord-349042-u9svz7pf 24 41 and and CC cord-349042-u9svz7pf 24 42 phage phage NN cord-349042-u9svz7pf 24 43 T7 T7 NNP cord-349042-u9svz7pf 24 44 RNA RNA NNP cord-349042-u9svz7pf 24 45 polymerase polymerase NN cord-349042-u9svz7pf 24 46 promoter promoter NN cord-349042-u9svz7pf 24 47 sequence sequence NN cord-349042-u9svz7pf 24 48 . . . cord-349042-u9svz7pf 25 1 The the DT cord-349042-u9svz7pf 25 2 poly(T poly(t JJ cord-349042-u9svz7pf 25 3 ) ) -RRB- cord-349042-u9svz7pf 25 4 portion portion NN cord-349042-u9svz7pf 25 5 of of IN cord-349042-u9svz7pf 25 6 the the DT cord-349042-u9svz7pf 25 7 primer primer NN cord-349042-u9svz7pf 25 8 is be VBZ cord-349042-u9svz7pf 25 9 used use VBN cord-349042-u9svz7pf 25 10 to to TO cord-349042-u9svz7pf 25 11 select select VB cord-349042-u9svz7pf 25 12 for for IN cord-349042-u9svz7pf 25 13 mRNA mRNA NNP cord-349042-u9svz7pf 25 14 species specie NNS cord-349042-u9svz7pf 25 15 that that WDT cord-349042-u9svz7pf 25 16 contain contain VBP cord-349042-u9svz7pf 25 17 a a DT cord-349042-u9svz7pf 25 18 poly(A poly(a JJ cord-349042-u9svz7pf 25 19 ) ) -RRB- cord-349042-u9svz7pf 25 20 tail tail NN cord-349042-u9svz7pf 25 21 , , , cord-349042-u9svz7pf 25 22 and and CC cord-349042-u9svz7pf 25 23 the the DT cord-349042-u9svz7pf 25 24 T7 T7 NNP cord-349042-u9svz7pf 25 25 promoter promoter NN cord-349042-u9svz7pf 25 26 portion portion NN cord-349042-u9svz7pf 25 27 of of IN cord-349042-u9svz7pf 25 28 the the DT cord-349042-u9svz7pf 25 29 primer primer NN cord-349042-u9svz7pf 25 30 is be VBZ cord-349042-u9svz7pf 25 31 used use VBN cord-349042-u9svz7pf 25 32 to to TO cord-349042-u9svz7pf 25 33 direct direct VB cord-349042-u9svz7pf 25 34 T7 t7 NN cord-349042-u9svz7pf 25 35 polymerase polymerase NN cord-349042-u9svz7pf 25 36 to to TO cord-349042-u9svz7pf 25 37 bind bind VB cord-349042-u9svz7pf 25 38 to to IN cord-349042-u9svz7pf 25 39 the the DT cord-349042-u9svz7pf 25 40 promoter promoter NN cord-349042-u9svz7pf 25 41 and and CC cord-349042-u9svz7pf 25 42 synthesize synthesize VB cord-349042-u9svz7pf 25 43 an an DT cord-349042-u9svz7pf 25 44 RNA RNA NNP cord-349042-u9svz7pf 25 45 copy copy NN cord-349042-u9svz7pf 25 46 of of IN cord-349042-u9svz7pf 25 47 the the DT cord-349042-u9svz7pf 25 48 cDNA cdna NN cord-349042-u9svz7pf 25 49 template template NN cord-349042-u9svz7pf 25 50 . . . cord-349042-u9svz7pf 26 1 This this DT cord-349042-u9svz7pf 26 2 Over over IN cord-349042-u9svz7pf 26 3 the the DT cord-349042-u9svz7pf 26 4 past past JJ cord-349042-u9svz7pf 26 5 ~25 ~25 CD cord-349042-u9svz7pf 26 6 years year NNS cord-349042-u9svz7pf 26 7 , , , cord-349042-u9svz7pf 26 8 particular particular JJ cord-349042-u9svz7pf 26 9 emphasis emphasis NN cord-349042-u9svz7pf 26 10 has have VBZ cord-349042-u9svz7pf 26 11 been be VBN cord-349042-u9svz7pf 26 12 placed place VBN cord-349042-u9svz7pf 26 13 on on IN cord-349042-u9svz7pf 26 14 understanding understand VBG cord-349042-u9svz7pf 26 15 the the DT cord-349042-u9svz7pf 26 16 transcriptome transcriptome NN cord-349042-u9svz7pf 26 17 of of IN cord-349042-u9svz7pf 26 18 cells cell NNS cord-349042-u9svz7pf 26 19 , , , cord-349042-u9svz7pf 26 20 with with IN cord-349042-u9svz7pf 26 21 the the DT cord-349042-u9svz7pf 26 22 knowledge knowledge NN cord-349042-u9svz7pf 26 23 that that WDT cord-349042-u9svz7pf 26 24 the the DT cord-349042-u9svz7pf 26 25 fundamental fundamental JJ cord-349042-u9svz7pf 26 26 player player NN cord-349042-u9svz7pf 26 27 in in IN cord-349042-u9svz7pf 26 28 converting convert VBG cord-349042-u9svz7pf 26 29 genomic genomic JJ cord-349042-u9svz7pf 26 30 information information NN cord-349042-u9svz7pf 26 31 into into IN cord-349042-u9svz7pf 26 32 biological biological JJ cord-349042-u9svz7pf 26 33 function function NN cord-349042-u9svz7pf 26 34 is be VBZ cord-349042-u9svz7pf 26 35 RNA RNA NNP cord-349042-u9svz7pf 26 36 . . . cord-349042-u9svz7pf 27 1 Whether whether IN cord-349042-u9svz7pf 27 2 translated translate VBN cord-349042-u9svz7pf 27 3 into into IN cord-349042-u9svz7pf 27 4 protein protein NN cord-349042-u9svz7pf 27 5 or or CC cord-349042-u9svz7pf 27 6 functioning function VBG cord-349042-u9svz7pf 27 7 in in IN cord-349042-u9svz7pf 27 8 a a DT cord-349042-u9svz7pf 27 9 regulatory regulatory JJ cord-349042-u9svz7pf 27 10 manner manner NN cord-349042-u9svz7pf 27 11 , , , cord-349042-u9svz7pf 27 12 RNAs rna NNS cord-349042-u9svz7pf 27 13 underlie underlie VBP cord-349042-u9svz7pf 27 14 many many JJ cord-349042-u9svz7pf 27 15 aspects aspect NNS cord-349042-u9svz7pf 27 16 of of IN cord-349042-u9svz7pf 27 17 cellular cellular JJ cord-349042-u9svz7pf 27 18 function function NN cord-349042-u9svz7pf 27 19 , , , cord-349042-u9svz7pf 27 20 and and CC cord-349042-u9svz7pf 27 21 it -PRON- PRP cord-349042-u9svz7pf 27 22 has have VBZ cord-349042-u9svz7pf 27 23 become become VBN cord-349042-u9svz7pf 27 24 clear clear JJ cord-349042-u9svz7pf 27 25 that that IN cord-349042-u9svz7pf 27 26 their -PRON- PRP$ cord-349042-u9svz7pf 27 27 dysregulation dysregulation NN cord-349042-u9svz7pf 27 28 is be VBZ cord-349042-u9svz7pf 27 29 associated associate VBN cord-349042-u9svz7pf 27 30 with with IN cord-349042-u9svz7pf 27 31 various various JJ cord-349042-u9svz7pf 27 32 diseases disease NNS cord-349042-u9svz7pf 27 33 . . . cord-349042-u9svz7pf 28 1 Understanding understand VBG cord-349042-u9svz7pf 28 2 RNA RNA NNP cord-349042-u9svz7pf 28 3 biology biology NN cord-349042-u9svz7pf 28 4 requires require VBZ cord-349042-u9svz7pf 28 5 detection detection NN cord-349042-u9svz7pf 28 6 , , , cord-349042-u9svz7pf 28 7 quantification quantification NN cord-349042-u9svz7pf 28 8 , , , cord-349042-u9svz7pf 28 9 and and CC cord-349042-u9svz7pf 28 10 sequencespecific sequencespecific JJ cord-349042-u9svz7pf 28 11 characterization characterization NN cord-349042-u9svz7pf 28 12 of of IN cord-349042-u9svz7pf 28 13 the the DT cord-349042-u9svz7pf 28 14 composite composite JJ cord-349042-u9svz7pf 28 15 RNAs rna NNS cord-349042-u9svz7pf 28 16 . . . cord-349042-u9svz7pf 29 1 The the DT cord-349042-u9svz7pf 29 2 technical technical JJ cord-349042-u9svz7pf 29 3 evolution evolution NN cord-349042-u9svz7pf 29 4 of of IN cord-349042-u9svz7pf 29 5 cell cell NN cord-349042-u9svz7pf 29 6 biology biology NN cord-349042-u9svz7pf 29 7 methods method NNS cord-349042-u9svz7pf 29 8 has have VBZ cord-349042-u9svz7pf 29 9 resulted result VBN cord-349042-u9svz7pf 29 10 in in IN cord-349042-u9svz7pf 29 11 the the DT cord-349042-u9svz7pf 29 12 relatively relatively RB cord-349042-u9svz7pf 29 13 easy easy JJ cord-349042-u9svz7pf 29 14 analysis analysis NN cord-349042-u9svz7pf 29 15 of of IN cord-349042-u9svz7pf 29 16 RNA RNA NNP cord-349042-u9svz7pf 29 17 populations population NNS cord-349042-u9svz7pf 29 18 from from IN cord-349042-u9svz7pf 29 19 organs organ NNS cord-349042-u9svz7pf 29 20 and and CC cord-349042-u9svz7pf 29 21 tissues tissue NNS cord-349042-u9svz7pf 29 22 where where WRB cord-349042-u9svz7pf 29 23 there there EX cord-349042-u9svz7pf 29 24 are be VBP cord-349042-u9svz7pf 29 25 large large JJ cord-349042-u9svz7pf 29 26 amounts amount NNS cord-349042-u9svz7pf 29 27 of of IN cord-349042-u9svz7pf 29 28 RNA RNA NNP cord-349042-u9svz7pf 29 29 . . . cord-349042-u9svz7pf 30 1 However however RB cord-349042-u9svz7pf 30 2 , , , cord-349042-u9svz7pf 30 3 transcriptomics transcriptomic NNS cord-349042-u9svz7pf 30 4 analyses analysis NNS cord-349042-u9svz7pf 30 5 become become VBP cord-349042-u9svz7pf 30 6 problematic problematic JJ cord-349042-u9svz7pf 30 7 when when WRB cord-349042-u9svz7pf 30 8 smaller small JJR cord-349042-u9svz7pf 30 9 amounts amount NNS cord-349042-u9svz7pf 30 10 of of IN cord-349042-u9svz7pf 30 11 tissue tissue NN cord-349042-u9svz7pf 30 12 , , , cord-349042-u9svz7pf 30 13 such such JJ cord-349042-u9svz7pf 30 14 as as IN cord-349042-u9svz7pf 30 15 punch punch NN cord-349042-u9svz7pf 30 16 biopsies biopsy NNS cord-349042-u9svz7pf 30 17 , , , cord-349042-u9svz7pf 30 18 are be VBP cord-349042-u9svz7pf 30 19 used use VBN cord-349042-u9svz7pf 30 20 for for IN cord-349042-u9svz7pf 30 21 human human JJ cord-349042-u9svz7pf 30 22 diagnosis diagnosis NN cord-349042-u9svz7pf 30 23 , , , cord-349042-u9svz7pf 30 24 or or CC cord-349042-u9svz7pf 30 25 in in IN cord-349042-u9svz7pf 30 26 examinations examination NNS cord-349042-u9svz7pf 30 27 of of IN cord-349042-u9svz7pf 30 28 individual individual JJ cord-349042-u9svz7pf 30 29 single single JJ cord-349042-u9svz7pf 30 30 cells cell NNS cord-349042-u9svz7pf 30 31 where where WRB cord-349042-u9svz7pf 30 32 RNA RNA NNP cord-349042-u9svz7pf 30 33 is be VBZ cord-349042-u9svz7pf 30 34 present present JJ cord-349042-u9svz7pf 30 35 in in IN cord-349042-u9svz7pf 30 36 small small JJ cord-349042-u9svz7pf 30 37 amounts amount NNS cord-349042-u9svz7pf 30 38 and and CC cord-349042-u9svz7pf 30 39 needs need VBZ cord-349042-u9svz7pf 30 40 to to TO cord-349042-u9svz7pf 30 41 be be VB cord-349042-u9svz7pf 30 42 amplified amplify VBN cord-349042-u9svz7pf 30 43 to to IN cord-349042-u9svz7pf 30 44 levels level NNS cord-349042-u9svz7pf 30 45 that that WDT cord-349042-u9svz7pf 30 46 can can MD cord-349042-u9svz7pf 30 47 be be VB cord-349042-u9svz7pf 30 48 experimentally experimentally RB cord-349042-u9svz7pf 30 49 analyzed analyze VBN cord-349042-u9svz7pf 30 50 . . . cord-349042-u9svz7pf 31 1 For for IN cord-349042-u9svz7pf 31 2 example example NN cord-349042-u9svz7pf 31 3 , , , cord-349042-u9svz7pf 31 4 there there EX cord-349042-u9svz7pf 31 5 are be VBP cord-349042-u9svz7pf 31 6 ~10,000 ~10,000 CD cord-349042-u9svz7pf 31 7 expressed express VBN cord-349042-u9svz7pf 31 8 genes gene NNS cord-349042-u9svz7pf 31 9 in in IN cord-349042-u9svz7pf 31 10 most most JJS cord-349042-u9svz7pf 31 11 cells cell NNS cord-349042-u9svz7pf 31 12 1 1 CD cord-349042-u9svz7pf 31 13 , , , cord-349042-u9svz7pf 31 14 giving give VBG cord-349042-u9svz7pf 31 15 rise rise NN cord-349042-u9svz7pf 31 16 to to IN cord-349042-u9svz7pf 31 17 300,000 300,000 CD cord-349042-u9svz7pf 31 18 total total NN cord-349042-u9svz7pf 31 19 mRNA mrna NN cord-349042-u9svz7pf 31 20 molecules molecule NNS cord-349042-u9svz7pf 31 21 ; ; : cord-349042-u9svz7pf 31 22 thus thus RB cord-349042-u9svz7pf 31 23 the the DT cord-349042-u9svz7pf 31 24 average average JJ cord-349042-u9svz7pf 31 25 number number NN cord-349042-u9svz7pf 31 26 of of IN cord-349042-u9svz7pf 31 27 RNA RNA NNP cord-349042-u9svz7pf 31 28 molecules molecule NNS cord-349042-u9svz7pf 31 29 is be VBZ cord-349042-u9svz7pf 31 30 30 30 CD cord-349042-u9svz7pf 31 31 per per IN cord-349042-u9svz7pf 31 32 gene gene NN cord-349042-u9svz7pf 31 33 , , , cord-349042-u9svz7pf 31 34 with with IN cord-349042-u9svz7pf 31 35 some some DT cord-349042-u9svz7pf 31 36 genes gene NNS cord-349042-u9svz7pf 31 37 transcribed transcribe VBN cord-349042-u9svz7pf 31 38 into into IN cord-349042-u9svz7pf 31 39 thousands thousand NNS cord-349042-u9svz7pf 31 40 of of IN cord-349042-u9svz7pf 31 41 mRNAs mrnas CD cord-349042-u9svz7pf 31 42 , , , cord-349042-u9svz7pf 31 43 and and CC cord-349042-u9svz7pf 31 44 others other NNS cord-349042-u9svz7pf 31 45 into into IN cord-349042-u9svz7pf 31 46 fewer few JJR cord-349042-u9svz7pf 31 47 than than IN cord-349042-u9svz7pf 31 48 ten ten CD cord-349042-u9svz7pf 31 49 . . . cord-349042-u9svz7pf 32 1 One one CD cord-349042-u9svz7pf 32 2 way way NN cord-349042-u9svz7pf 32 3 to to TO cord-349042-u9svz7pf 32 4 detect detect VB cord-349042-u9svz7pf 32 5 such such JJ cord-349042-u9svz7pf 32 6 low low JJ cord-349042-u9svz7pf 32 7 - - HYPH cord-349042-u9svz7pf 32 8 abundance abundance NN cord-349042-u9svz7pf 32 9 RNAs rna NNS cord-349042-u9svz7pf 32 10 is be VBZ cord-349042-u9svz7pf 32 11 to to TO cord-349042-u9svz7pf 32 12 amplify amplify VB cord-349042-u9svz7pf 32 13 their -PRON- PRP$ cord-349042-u9svz7pf 32 14 numbers number NNS cord-349042-u9svz7pf 32 15 . . . cord-349042-u9svz7pf 33 1 The the DT cord-349042-u9svz7pf 33 2 two two CD cord-349042-u9svz7pf 33 3 most most RBS cord-349042-u9svz7pf 33 4 important important JJ cord-349042-u9svz7pf 33 5 considerations consideration NNS cord-349042-u9svz7pf 33 6 in in IN cord-349042-u9svz7pf 33 7 the the DT cord-349042-u9svz7pf 33 8 design design NN cord-349042-u9svz7pf 33 9 of of IN cord-349042-u9svz7pf 33 10 RNA RNA NNP cord-349042-u9svz7pf 33 11 amplification amplification NN cord-349042-u9svz7pf 33 12 methods method NNS cord-349042-u9svz7pf 33 13 are be VBP cord-349042-u9svz7pf 33 14 the the DT cord-349042-u9svz7pf 33 15 yield yield NN cord-349042-u9svz7pf 33 16 of of IN cord-349042-u9svz7pf 33 17 amplification amplification NN cord-349042-u9svz7pf 33 18 and and CC cord-349042-u9svz7pf 33 19 how how WRB cord-349042-u9svz7pf 33 20 well well RB cord-349042-u9svz7pf 33 21 the the DT cord-349042-u9svz7pf 33 22 relative relative JJ cord-349042-u9svz7pf 33 23 abundances abundance NNS cord-349042-u9svz7pf 33 24 of of IN cord-349042-u9svz7pf 33 25 the the DT cord-349042-u9svz7pf 33 26 different different JJ cord-349042-u9svz7pf 33 27 RNAs rna NNS cord-349042-u9svz7pf 33 28 present present VBP cord-349042-u9svz7pf 33 29 The the DT cord-349042-u9svz7pf 33 30 successes success NNS cord-349042-u9svz7pf 33 31 and and CC cord-349042-u9svz7pf 33 32 future future JJ cord-349042-u9svz7pf 33 33 prospects prospect NNS cord-349042-u9svz7pf 33 34 of of IN cord-349042-u9svz7pf 33 35 the the DT cord-349042-u9svz7pf 33 36 linear linear JJ cord-349042-u9svz7pf 33 37 antisense antisense NN cord-349042-u9svz7pf 33 38 RNA RNA NNP cord-349042-u9svz7pf 33 39 amplification amplification NN cord-349042-u9svz7pf 33 40 methodology methodology NN cord-349042-u9svz7pf 33 41 It -PRON- PRP cord-349042-u9svz7pf 33 42 has have VBZ cord-349042-u9svz7pf 33 43 been be VBN cord-349042-u9svz7pf 33 44 over over IN cord-349042-u9svz7pf 33 45 a a DT cord-349042-u9svz7pf 33 46 quarter quarter NN cord-349042-u9svz7pf 33 47 of of IN cord-349042-u9svz7pf 33 48 a a DT cord-349042-u9svz7pf 33 49 century century NN cord-349042-u9svz7pf 33 50 since since IN cord-349042-u9svz7pf 33 51 the the DT cord-349042-u9svz7pf 33 52 introduction introduction NN cord-349042-u9svz7pf 33 53 of of IN cord-349042-u9svz7pf 33 54 the the DT cord-349042-u9svz7pf 33 55 linear linear NNP cord-349042-u9svz7pf 33 56 RNA RNA NNP cord-349042-u9svz7pf 33 57 amplification amplification NN cord-349042-u9svz7pf 33 58 methodology methodology NN cord-349042-u9svz7pf 33 59 known know VBN cord-349042-u9svz7pf 33 60 as as IN cord-349042-u9svz7pf 33 61 antisense antisense NNP cord-349042-u9svz7pf 33 62 RNA RNA NNP cord-349042-u9svz7pf 33 63 ( ( -LRB- cord-349042-u9svz7pf 33 64 aRNA arna NN cord-349042-u9svz7pf 33 65 ) ) -RRB- cord-349042-u9svz7pf 33 66 amplification amplification NN cord-349042-u9svz7pf 33 67 . . . cord-349042-u9svz7pf 34 1 Whereas whereas IN cord-349042-u9svz7pf 34 2 most most JJS cord-349042-u9svz7pf 34 3 molecular molecular JJ cord-349042-u9svz7pf 34 4 biology biology NN cord-349042-u9svz7pf 34 5 techniques technique NNS cord-349042-u9svz7pf 34 6 are be VBP cord-349042-u9svz7pf 34 7 rapidly rapidly RB cord-349042-u9svz7pf 34 8 replaced replace VBN cord-349042-u9svz7pf 34 9 owing owe VBG cord-349042-u9svz7pf 34 10 to to IN cord-349042-u9svz7pf 34 11 the the DT cord-349042-u9svz7pf 34 12 fast fast RB cord-349042-u9svz7pf 34 13 - - HYPH cord-349042-u9svz7pf 34 14 moving move VBG cord-349042-u9svz7pf 34 15 nature nature NN cord-349042-u9svz7pf 34 16 of of IN cord-349042-u9svz7pf 34 17 development development NN cord-349042-u9svz7pf 34 18 in in IN cord-349042-u9svz7pf 34 19 the the DT cord-349042-u9svz7pf 34 20 field field NN cord-349042-u9svz7pf 34 21 , , , cord-349042-u9svz7pf 34 22 the the DT cord-349042-u9svz7pf 34 23 aRNA arna NN cord-349042-u9svz7pf 34 24 procedure procedure NN cord-349042-u9svz7pf 34 25 has have VBZ cord-349042-u9svz7pf 34 26 become become VBN cord-349042-u9svz7pf 34 27 a a DT cord-349042-u9svz7pf 34 28 base base NN cord-349042-u9svz7pf 34 29 that that WDT cord-349042-u9svz7pf 34 30 can can MD cord-349042-u9svz7pf 34 31 be be VB cord-349042-u9svz7pf 34 32 built build VBN cord-349042-u9svz7pf 34 33 upon upon IN cord-349042-u9svz7pf 34 34 through through IN cord-349042-u9svz7pf 34 35 varied varied JJ cord-349042-u9svz7pf 34 36 uses use NNS cord-349042-u9svz7pf 34 37 of of IN cord-349042-u9svz7pf 34 38 the the DT cord-349042-u9svz7pf 34 39 technology technology NN cord-349042-u9svz7pf 34 40 . . . cord-349042-u9svz7pf 35 1 The the DT cord-349042-u9svz7pf 35 2 technique technique NN cord-349042-u9svz7pf 35 3 was be VBD cord-349042-u9svz7pf 35 4 originally originally RB cord-349042-u9svz7pf 35 5 developed develop VBN cord-349042-u9svz7pf 35 6 to to TO cord-349042-u9svz7pf 35 7 assess assess VB cord-349042-u9svz7pf 35 8 RNA RNA NNP cord-349042-u9svz7pf 35 9 populations population NNS cord-349042-u9svz7pf 35 10 from from IN cord-349042-u9svz7pf 35 11 small small JJ cord-349042-u9svz7pf 35 12 amounts amount NNS cord-349042-u9svz7pf 35 13 of of IN cord-349042-u9svz7pf 35 14 starting start VBG cord-349042-u9svz7pf 35 15 material material NN cord-349042-u9svz7pf 35 16 , , , cord-349042-u9svz7pf 35 17 including include VBG cord-349042-u9svz7pf 35 18 single single JJ cord-349042-u9svz7pf 35 19 cells cell NNS cord-349042-u9svz7pf 35 20 , , , cord-349042-u9svz7pf 35 21 but but CC cord-349042-u9svz7pf 35 22 over over IN cord-349042-u9svz7pf 35 23 time time NN cord-349042-u9svz7pf 35 24 its -PRON- PRP$ cord-349042-u9svz7pf 35 25 use use NN cord-349042-u9svz7pf 35 26 has have VBZ cord-349042-u9svz7pf 35 27 evolved evolve VBN cord-349042-u9svz7pf 35 28 to to TO cord-349042-u9svz7pf 35 29 include include VB cord-349042-u9svz7pf 35 30 the the DT cord-349042-u9svz7pf 35 31 detection detection NN cord-349042-u9svz7pf 35 32 of of IN cord-349042-u9svz7pf 35 33 various various JJ cord-349042-u9svz7pf 35 34 cellular cellular JJ cord-349042-u9svz7pf 35 35 entities entity NNS cord-349042-u9svz7pf 35 36 such such JJ cord-349042-u9svz7pf 35 37 as as IN cord-349042-u9svz7pf 35 38 proteins protein NNS cord-349042-u9svz7pf 35 39 , , , cord-349042-u9svz7pf 35 40 RNA rna NN cord-349042-u9svz7pf 35 41 - - HYPH cord-349042-u9svz7pf 35 42 binding bind VBG cord-349042-u9svz7pf 35 43 - - HYPH cord-349042-u9svz7pf 35 44 protein protein NN cord-349042-u9svz7pf 35 45 - - HYPH cord-349042-u9svz7pf 35 46 associated associate VBN cord-349042-u9svz7pf 35 47 cargoes cargo NNS cord-349042-u9svz7pf 35 48 , , , cord-349042-u9svz7pf 35 49 and and CC cord-349042-u9svz7pf 35 50 genomic genomic JJ cord-349042-u9svz7pf 35 51 DNA dna NN cord-349042-u9svz7pf 35 52 . . . cord-349042-u9svz7pf 36 1 In in IN cord-349042-u9svz7pf 36 2 this this DT cord-349042-u9svz7pf 36 3 Perspective perspective NN cord-349042-u9svz7pf 36 4 we -PRON- PRP cord-349042-u9svz7pf 36 5 detail detail VBP cord-349042-u9svz7pf 36 6 the the DT cord-349042-u9svz7pf 36 7 linear linear JJ cord-349042-u9svz7pf 36 8 aRNA aRNA HYPH cord-349042-u9svz7pf 36 9 amplification amplification NN cord-349042-u9svz7pf 36 10 procedure procedure NN cord-349042-u9svz7pf 36 11 and and CC cord-349042-u9svz7pf 36 12 its -PRON- PRP$ cord-349042-u9svz7pf 36 13 use use NN cord-349042-u9svz7pf 36 14 in in IN cord-349042-u9svz7pf 36 15 assessing assess VBG cord-349042-u9svz7pf 36 16 various various JJ cord-349042-u9svz7pf 36 17 components component NNS cord-349042-u9svz7pf 36 18 of of IN cord-349042-u9svz7pf 36 19 a a DT cord-349042-u9svz7pf 36 20 cell cell NN cord-349042-u9svz7pf 36 21 's 's POS cord-349042-u9svz7pf 36 22 chemical chemical JJ cord-349042-u9svz7pf 36 23 phenotype phenotype NN cord-349042-u9svz7pf 36 24 . . . cord-349042-u9svz7pf 37 1 This this DT cord-349042-u9svz7pf 37 2 procedure procedure NN cord-349042-u9svz7pf 37 3 is be VBZ cord-349042-u9svz7pf 37 4 particularly particularly RB cord-349042-u9svz7pf 37 5 useful useful JJ cord-349042-u9svz7pf 37 6 in in IN cord-349042-u9svz7pf 37 7 efforts effort NNS cord-349042-u9svz7pf 37 8 to to TO cord-349042-u9svz7pf 37 9 multiplex multiplex VB cord-349042-u9svz7pf 37 10 the the DT cord-349042-u9svz7pf 37 11 simultaneous simultaneous JJ cord-349042-u9svz7pf 37 12 detection detection NN cord-349042-u9svz7pf 37 13 of of IN cord-349042-u9svz7pf 37 14 various various JJ cord-349042-u9svz7pf 37 15 cellular cellular JJ cord-349042-u9svz7pf 37 16 processes process NNS cord-349042-u9svz7pf 37 17 . . . cord-349042-u9svz7pf 38 1 These these DT cord-349042-u9svz7pf 38 2 efforts effort NNS cord-349042-u9svz7pf 38 3 are be VBP cord-349042-u9svz7pf 38 4 necessary necessary JJ cord-349042-u9svz7pf 38 5 to to TO cord-349042-u9svz7pf 38 6 identify identify VB cord-349042-u9svz7pf 38 7 the the DT cord-349042-u9svz7pf 38 8 quantitative quantitative JJ cord-349042-u9svz7pf 38 9 chemical chemical NN cord-349042-u9svz7pf 38 10 phenotype phenotype NN cord-349042-u9svz7pf 38 11 of of IN cord-349042-u9svz7pf 38 12 cells cell NNS cord-349042-u9svz7pf 38 13 that that WDT cord-349042-u9svz7pf 38 14 underlies underlie VBZ cord-349042-u9svz7pf 38 15 cellular cellular JJ cord-349042-u9svz7pf 38 16 function function NN cord-349042-u9svz7pf 38 17 . . . cord-349042-u9svz7pf 39 1 in in IN cord-349042-u9svz7pf 39 2 vitro vitro FW cord-349042-u9svz7pf 39 3 transcription transcription NN cord-349042-u9svz7pf 39 4 by by IN cord-349042-u9svz7pf 39 5 T7 T7 NNP cord-349042-u9svz7pf 39 6 RNA RNA NNP cord-349042-u9svz7pf 39 7 polymerase polymerase NN cord-349042-u9svz7pf 39 8 . . . cord-349042-u9svz7pf 40 1 If if IN cord-349042-u9svz7pf 40 2 necessary necessary JJ cord-349042-u9svz7pf 40 3 , , , cord-349042-u9svz7pf 40 4 a a DT cord-349042-u9svz7pf 40 5 third third JJ cord-349042-u9svz7pf 40 6 round round NN cord-349042-u9svz7pf 40 7 of of IN cord-349042-u9svz7pf 40 8 aRNA arna NN cord-349042-u9svz7pf 40 9 amplification amplification NN cord-349042-u9svz7pf 40 10 can can MD cord-349042-u9svz7pf 40 11 be be VB cord-349042-u9svz7pf 40 12 performed perform VBN cord-349042-u9svz7pf 40 13 after after IN cord-349042-u9svz7pf 40 14 the the DT cord-349042-u9svz7pf 40 15 secondround secondround NN cord-349042-u9svz7pf 40 16 amplification amplification NN cord-349042-u9svz7pf 40 17 procedure procedure NN cord-349042-u9svz7pf 40 18 ( ( -LRB- cord-349042-u9svz7pf 40 19 Fig Fig NNP cord-349042-u9svz7pf 40 20 . . . cord-349042-u9svz7pf 40 21 1 1 CD cord-349042-u9svz7pf 40 22 ) ) -RRB- cord-349042-u9svz7pf 40 23 . . . cord-349042-u9svz7pf 41 1 After after IN cord-349042-u9svz7pf 41 2 one one CD cord-349042-u9svz7pf 41 3 round round NN cord-349042-u9svz7pf 41 4 of of IN cord-349042-u9svz7pf 41 5 amplification amplification NN cord-349042-u9svz7pf 41 6 , , , cord-349042-u9svz7pf 41 7 ~2,000-fold ~2,000-fold JJ cord-349042-u9svz7pf 41 8 quantities quantity NNS cord-349042-u9svz7pf 41 9 of of IN cord-349042-u9svz7pf 41 10 original original JJ cord-349042-u9svz7pf 41 11 mRNA mrna NN cord-349042-u9svz7pf 41 12 can can MD cord-349042-u9svz7pf 41 13 be be VB cord-349042-u9svz7pf 41 14 generated generate VBN cord-349042-u9svz7pf 41 15 . . . cord-349042-u9svz7pf 42 1 After after IN cord-349042-u9svz7pf 42 2 the the DT cord-349042-u9svz7pf 42 3 second second JJ cord-349042-u9svz7pf 42 4 round round NN cord-349042-u9svz7pf 42 5 , , , cord-349042-u9svz7pf 42 6 > > XX cord-349042-u9svz7pf 42 7 1 1 CD cord-349042-u9svz7pf 42 8 × × NN cord-349042-u9svz7pf 42 9 10 10 CD cord-349042-u9svz7pf 42 10 6 6 CD cord-349042-u9svz7pf 43 1 -fold -fold NNP cord-349042-u9svz7pf 43 2 RNA RNA NNP cord-349042-u9svz7pf 43 3 amplification amplification NN cord-349042-u9svz7pf 43 4 can can MD cord-349042-u9svz7pf 43 5 be be VB cord-349042-u9svz7pf 43 6 achieved achieve VBN cord-349042-u9svz7pf 43 7 3 3 CD cord-349042-u9svz7pf 43 8 , , , cord-349042-u9svz7pf 43 9 which which WDT cord-349042-u9svz7pf 43 10 is be VBZ cord-349042-u9svz7pf 43 11 adequate adequate JJ cord-349042-u9svz7pf 43 12 for for IN cord-349042-u9svz7pf 43 13 the the DT cord-349042-u9svz7pf 43 14 generation generation NN cord-349042-u9svz7pf 43 15 of of IN cord-349042-u9svz7pf 43 16 microarray microarray NN cord-349042-u9svz7pf 43 17 probes probe NNS cord-349042-u9svz7pf 43 18 4 4 CD cord-349042-u9svz7pf 43 19 or or CC cord-349042-u9svz7pf 43 20 a a DT cord-349042-u9svz7pf 43 21 sequencing sequencing NN cord-349042-u9svz7pf 43 22 library library NN cord-349042-u9svz7pf 43 23 4 4 CD cord-349042-u9svz7pf 43 24 , , , cord-349042-u9svz7pf 43 25 5 5 CD cord-349042-u9svz7pf 43 26 . . . cord-349042-u9svz7pf 44 1 This this DT cord-349042-u9svz7pf 44 2 procedure procedure NN cord-349042-u9svz7pf 44 3 has have VBZ cord-349042-u9svz7pf 44 4 proven prove VBN cord-349042-u9svz7pf 44 5 useful useful JJ cord-349042-u9svz7pf 44 6 for for IN cord-349042-u9svz7pf 44 7 amplification amplification NN cord-349042-u9svz7pf 44 8 of of IN cord-349042-u9svz7pf 44 9 RNA RNA NNP cord-349042-u9svz7pf 44 10 from from IN cord-349042-u9svz7pf 44 11 single single JJ cord-349042-u9svz7pf 44 12 cells cell NNS cord-349042-u9svz7pf 44 13 , , , cord-349042-u9svz7pf 44 14 which which WDT cord-349042-u9svz7pf 44 15 contain contain VBP cord-349042-u9svz7pf 44 16 only only RB cord-349042-u9svz7pf 44 17 ~10 ~10 CD cord-349042-u9svz7pf 44 18 picograms picogram NNS cord-349042-u9svz7pf 44 19 of of IN cord-349042-u9svz7pf 44 20 total total JJ cord-349042-u9svz7pf 44 21 RNA RNA NNP cord-349042-u9svz7pf 44 22 [ [ -LRB- cord-349042-u9svz7pf 44 23 6 6 CD cord-349042-u9svz7pf 44 24 ] ] -RRB- cord-349042-u9svz7pf 44 25 [ [ -LRB- cord-349042-u9svz7pf 44 26 7 7 CD cord-349042-u9svz7pf 44 27 ] ] -RRB- cord-349042-u9svz7pf 44 28 [ [ -LRB- cord-349042-u9svz7pf 44 29 8 8 CD cord-349042-u9svz7pf 44 30 ] ] -RRB- cord-349042-u9svz7pf 44 31 . . . cord-349042-u9svz7pf 45 1 Examination examination NN cord-349042-u9svz7pf 45 2 of of IN cord-349042-u9svz7pf 45 3 expression expression NN cord-349042-u9svz7pf 45 4 profiles profile NNS cord-349042-u9svz7pf 45 5 of of IN cord-349042-u9svz7pf 45 6 single single JJ cord-349042-u9svz7pf 45 7 live live JJ cord-349042-u9svz7pf 45 8 cells cell NNS cord-349042-u9svz7pf 45 9 has have VBZ cord-349042-u9svz7pf 45 10 shown show VBN cord-349042-u9svz7pf 45 11 that that IN cord-349042-u9svz7pf 45 12 linear linear JJ cord-349042-u9svz7pf 45 13 aRNA arna NN cord-349042-u9svz7pf 45 14 amplification amplification NN cord-349042-u9svz7pf 45 15 neither neither CC cord-349042-u9svz7pf 45 16 results result VBZ cord-349042-u9svz7pf 45 17 in in IN cord-349042-u9svz7pf 45 18 occurs occur NNS cord-349042-u9svz7pf 45 19 after after IN cord-349042-u9svz7pf 45 20 synthesis synthesis NN cord-349042-u9svz7pf 45 21 of of IN cord-349042-u9svz7pf 45 22 double double RB cord-349042-u9svz7pf 45 23 - - HYPH cord-349042-u9svz7pf 45 24 stranded strand VBN cord-349042-u9svz7pf 45 25 cDNA cdna NN cord-349042-u9svz7pf 45 26 , , , cord-349042-u9svz7pf 45 27 when when WRB cord-349042-u9svz7pf 45 28 T7 T7 NNP cord-349042-u9svz7pf 45 29 RNA RNA NNP cord-349042-u9svz7pf 45 30 polymerase polymerase NN cord-349042-u9svz7pf 45 31 is be VBZ cord-349042-u9svz7pf 45 32 added add VBN cord-349042-u9svz7pf 45 33 and and CC cord-349042-u9svz7pf 45 34 aRNA arna NN cord-349042-u9svz7pf 45 35 is be VBZ cord-349042-u9svz7pf 45 36 transcribed transcribe VBN cord-349042-u9svz7pf 45 37 from from IN cord-349042-u9svz7pf 45 38 the the DT cord-349042-u9svz7pf 45 39 cDNA cdna NN cord-349042-u9svz7pf 45 40 template template NN cord-349042-u9svz7pf 45 41 . . . cord-349042-u9svz7pf 46 1 There there EX cord-349042-u9svz7pf 46 2 is be VBZ cord-349042-u9svz7pf 46 3 then then RB cord-349042-u9svz7pf 46 4 a a DT cord-349042-u9svz7pf 46 5 second second JJ cord-349042-u9svz7pf 46 6 round round NN cord-349042-u9svz7pf 46 7 of of IN cord-349042-u9svz7pf 46 8 amplification amplification NN cord-349042-u9svz7pf 46 9 , , , cord-349042-u9svz7pf 46 10 in in IN cord-349042-u9svz7pf 46 11 which which WDT cord-349042-u9svz7pf 46 12 aRNA arna NN cord-349042-u9svz7pf 46 13 is be VBZ cord-349042-u9svz7pf 46 14 amplified amplify VBN cord-349042-u9svz7pf 46 15 through through IN cord-349042-u9svz7pf 46 16 conversion conversion NN cord-349042-u9svz7pf 46 17 of of IN cord-349042-u9svz7pf 46 18 aRNA arna NN cord-349042-u9svz7pf 46 19 into into IN cord-349042-u9svz7pf 46 20 cDNA cdna NN cord-349042-u9svz7pf 46 21 , , , cord-349042-u9svz7pf 46 22 with with IN cord-349042-u9svz7pf 46 23 random random JJ cord-349042-u9svz7pf 46 24 hexanucleotide hexanucleotide NN cord-349042-u9svz7pf 46 25 primers primer NNS cord-349042-u9svz7pf 46 26 used use VBN cord-349042-u9svz7pf 46 27 to to TO cord-349042-u9svz7pf 46 28 intitiate intitiate NNP cord-349042-u9svz7pf 46 29 cDNA cDNA NNP cord-349042-u9svz7pf 46 30 synthesis synthesis NN cord-349042-u9svz7pf 46 31 . . . cord-349042-u9svz7pf 47 1 Random random JJ cord-349042-u9svz7pf 47 2 primers primer NNS cord-349042-u9svz7pf 47 3 are be VBP cord-349042-u9svz7pf 47 4 used use VBN cord-349042-u9svz7pf 47 5 because because IN cord-349042-u9svz7pf 47 6 of of IN cord-349042-u9svz7pf 47 7 their -PRON- PRP$ cord-349042-u9svz7pf 47 8 random random JJ cord-349042-u9svz7pf 47 9 annealing annealing NN cord-349042-u9svz7pf 47 10 property property NN cord-349042-u9svz7pf 47 11 , , , cord-349042-u9svz7pf 47 12 which which WDT cord-349042-u9svz7pf 47 13 allows allow VBZ cord-349042-u9svz7pf 47 14 them -PRON- PRP cord-349042-u9svz7pf 47 15 to to TO cord-349042-u9svz7pf 47 16 prime prime NNP cord-349042-u9svz7pf 47 17 any any DT cord-349042-u9svz7pf 47 18 RNA RNA NNP cord-349042-u9svz7pf 47 19 species species NN cord-349042-u9svz7pf 47 20 for for IN cord-349042-u9svz7pf 47 21 reverse reverse JJ cord-349042-u9svz7pf 47 22 transcription transcription NN cord-349042-u9svz7pf 47 23 into into IN cord-349042-u9svz7pf 47 24 cDNA cdna NN cord-349042-u9svz7pf 47 25 . . . cord-349042-u9svz7pf 48 1 The the DT cord-349042-u9svz7pf 48 2 oligo(dT)-T7 oligo(dt)-t7 JJ cord-349042-u9svz7pf 48 3 primer primer NN cord-349042-u9svz7pf 48 4 serves serve VBZ cord-349042-u9svz7pf 48 5 as as IN cord-349042-u9svz7pf 48 6 a a DT cord-349042-u9svz7pf 48 7 primer primer NN cord-349042-u9svz7pf 48 8 for for IN cord-349042-u9svz7pf 48 9 second second JJ cord-349042-u9svz7pf 48 10 - - HYPH cord-349042-u9svz7pf 48 11 strand strand NN cord-349042-u9svz7pf 48 12 cDNA cdna NN cord-349042-u9svz7pf 48 13 synthesis synthesis NN cord-349042-u9svz7pf 48 14 , , , cord-349042-u9svz7pf 48 15 in in IN cord-349042-u9svz7pf 48 16 which which WDT cord-349042-u9svz7pf 48 17 the the DT cord-349042-u9svz7pf 48 18 T7 T7 NNP cord-349042-u9svz7pf 48 19 promoter promoter NN cord-349042-u9svz7pf 48 20 is be VBZ cord-349042-u9svz7pf 48 21 added add VBN cord-349042-u9svz7pf 48 22 to to IN cord-349042-u9svz7pf 48 23 the the DT cord-349042-u9svz7pf 48 24 double double RB cord-349042-u9svz7pf 48 25 - - HYPH cord-349042-u9svz7pf 48 26 stranded strand VBN cord-349042-u9svz7pf 48 27 cDNA cdna NN cord-349042-u9svz7pf 48 28 at at IN cord-349042-u9svz7pf 48 29 the the DT cord-349042-u9svz7pf 48 30 end end NN cord-349042-u9svz7pf 48 31 of of IN cord-349042-u9svz7pf 48 32 the the DT cord-349042-u9svz7pf 48 33 second second JJ cord-349042-u9svz7pf 48 34 round round NN cord-349042-u9svz7pf 48 35 , , , cord-349042-u9svz7pf 48 36 thereby thereby RB cord-349042-u9svz7pf 48 37 allowing allow VBG cord-349042-u9svz7pf 48 38 subsequent subsequent JJ cord-349042-u9svz7pf 48 39 second second JJ cord-349042-u9svz7pf 48 40 - - HYPH cord-349042-u9svz7pf 48 41 round round NN cord-349042-u9svz7pf 48 42 aRNA arna NN cord-349042-u9svz7pf 49 1 In in IN cord-349042-u9svz7pf 49 2 the the DT cord-349042-u9svz7pf 49 3 first first JJ cord-349042-u9svz7pf 49 4 round round NN cord-349042-u9svz7pf 49 5 , , , cord-349042-u9svz7pf 49 6 first first JJ cord-349042-u9svz7pf 49 7 - - HYPH cord-349042-u9svz7pf 49 8 strand strand NN cord-349042-u9svz7pf 49 9 cDNA cdna NN cord-349042-u9svz7pf 49 10 synthesis synthesis NN cord-349042-u9svz7pf 49 11 by by IN cord-349042-u9svz7pf 49 12 reverse reverse JJ cord-349042-u9svz7pf 49 13 transcription transcription NN cord-349042-u9svz7pf 49 14 ( ( -LRB- cord-349042-u9svz7pf 49 15 RT RT NNP cord-349042-u9svz7pf 49 16 ) ) -RRB- cord-349042-u9svz7pf 49 17 is be VBZ cord-349042-u9svz7pf 49 18 primed prime VBN cord-349042-u9svz7pf 49 19 from from IN cord-349042-u9svz7pf 49 20 mRNA mRNA NNP cord-349042-u9svz7pf 49 21 after after IN cord-349042-u9svz7pf 49 22 oligo(dT)-T7 oligo(dt)-t7 JJ cord-349042-u9svz7pf 49 23 primer primer NN cord-349042-u9svz7pf 49 24 anneals anneal NNS cord-349042-u9svz7pf 49 25 to to IN cord-349042-u9svz7pf 49 26 the the DT cord-349042-u9svz7pf 49 27 poly(A poly(a JJ cord-349042-u9svz7pf 49 28 ) ) -RRB- cord-349042-u9svz7pf 49 29 tail tail NN cord-349042-u9svz7pf 49 30 of of IN cord-349042-u9svz7pf 49 31 mRNA mrna NN cord-349042-u9svz7pf 49 32 . . . cord-349042-u9svz7pf 50 1 RNase RNase NNP cord-349042-u9svz7pf 50 2 H H NNP cord-349042-u9svz7pf 50 3 is be VBZ cord-349042-u9svz7pf 50 4 then then RB cord-349042-u9svz7pf 50 5 used use VBN cord-349042-u9svz7pf 50 6 to to TO cord-349042-u9svz7pf 50 7 digest digest VB cord-349042-u9svz7pf 50 8 portions portion NNS cord-349042-u9svz7pf 50 9 of of IN cord-349042-u9svz7pf 50 10 the the DT cord-349042-u9svz7pf 50 11 bound bind VBN cord-349042-u9svz7pf 50 12 mRNA mrna NN cord-349042-u9svz7pf 50 13 to to TO cord-349042-u9svz7pf 50 14 create create VB cord-349042-u9svz7pf 50 15 RNA rna NN cord-349042-u9svz7pf 50 16 fragments fragment NNS cord-349042-u9svz7pf 50 17 that that WDT cord-349042-u9svz7pf 50 18 serve serve VBP cord-349042-u9svz7pf 50 19 to to IN cord-349042-u9svz7pf 50 20 prime prime JJ cord-349042-u9svz7pf 50 21 second second JJ cord-349042-u9svz7pf 50 22 - - HYPH cord-349042-u9svz7pf 50 23 strand strand NN cord-349042-u9svz7pf 50 24 cDNA cdna NN cord-349042-u9svz7pf 50 25 by by IN cord-349042-u9svz7pf 50 26 DNA dna NN cord-349042-u9svz7pf 50 27 polymerase polymerase NN cord-349042-u9svz7pf 50 28 ( ( -LRB- cord-349042-u9svz7pf 50 29 pol pol NN cord-349042-u9svz7pf 50 30 ) ) -RRB- cord-349042-u9svz7pf 50 31 . . . cord-349042-u9svz7pf 51 1 Finally finally RB cord-349042-u9svz7pf 51 2 , , , cord-349042-u9svz7pf 51 3 aRNA arna NN cord-349042-u9svz7pf 51 4 is be VBZ cord-349042-u9svz7pf 51 5 amplified amplify VBN cord-349042-u9svz7pf 51 6 via via IN cord-349042-u9svz7pf 51 7 linear linear NNP cord-349042-u9svz7pf 51 8 in in IN cord-349042-u9svz7pf 51 9 vitro vitro FW cord-349042-u9svz7pf 51 10 transcription transcription NN cord-349042-u9svz7pf 51 11 by by IN cord-349042-u9svz7pf 51 12 T7 T7 NNP cord-349042-u9svz7pf 51 13 RNA RNA NNP cord-349042-u9svz7pf 51 14 polymerase polymerase NN cord-349042-u9svz7pf 51 15 , , , cord-349042-u9svz7pf 51 16 using use VBG cord-349042-u9svz7pf 51 17 the the DT cord-349042-u9svz7pf 51 18 T7 T7 NNP cord-349042-u9svz7pf 51 19 RNA RNA NNP cord-349042-u9svz7pf 51 20 polymerase polymerase NN cord-349042-u9svz7pf 51 21 promoter promoter NN cord-349042-u9svz7pf 51 22 incorporated incorporate VBN cord-349042-u9svz7pf 51 23 in in IN cord-349042-u9svz7pf 51 24 the the DT cord-349042-u9svz7pf 51 25 double double RB cord-349042-u9svz7pf 51 26 - - HYPH cord-349042-u9svz7pf 51 27 stranded strand VBN cord-349042-u9svz7pf 51 28 cDNA cdna NN cord-349042-u9svz7pf 51 29 . . . cord-349042-u9svz7pf 52 1 In in IN cord-349042-u9svz7pf 52 2 the the DT cord-349042-u9svz7pf 52 3 second second JJ cord-349042-u9svz7pf 52 4 round round NN cord-349042-u9svz7pf 52 5 , , , cord-349042-u9svz7pf 52 6 first first JJ cord-349042-u9svz7pf 52 7 - - HYPH cord-349042-u9svz7pf 52 8 strand strand NN cord-349042-u9svz7pf 52 9 synthesis synthesis NN cord-349042-u9svz7pf 52 10 is be VBZ cord-349042-u9svz7pf 52 11 primed prime VBN cord-349042-u9svz7pf 52 12 by by IN cord-349042-u9svz7pf 52 13 random random JJ cord-349042-u9svz7pf 52 14 primers primer NNS cord-349042-u9svz7pf 52 15 instead instead RB cord-349042-u9svz7pf 52 16 of of IN cord-349042-u9svz7pf 52 17 the the DT cord-349042-u9svz7pf 52 18 oligo(dT)-T7 oligo(dt)-t7 JJ cord-349042-u9svz7pf 52 19 primer primer NN cord-349042-u9svz7pf 52 20 by by IN cord-349042-u9svz7pf 52 21 reverse reverse JJ cord-349042-u9svz7pf 52 22 transcriptase transcriptase NN cord-349042-u9svz7pf 52 23 using use VBG cord-349042-u9svz7pf 52 24 the the DT cord-349042-u9svz7pf 52 25 aRNA arna NN cord-349042-u9svz7pf 52 26 as as IN cord-349042-u9svz7pf 52 27 a a DT cord-349042-u9svz7pf 52 28 template template NN cord-349042-u9svz7pf 52 29 instead instead RB cord-349042-u9svz7pf 52 30 of of IN cord-349042-u9svz7pf 52 31 mRNA mRNA NNP cord-349042-u9svz7pf 52 32 . . . cord-349042-u9svz7pf 53 1 After after IN cord-349042-u9svz7pf 53 2 RNA RNA NNP cord-349042-u9svz7pf 53 3 denaturation denaturation NN cord-349042-u9svz7pf 53 4 , , , cord-349042-u9svz7pf 53 5 second second JJ cord-349042-u9svz7pf 53 6 - - HYPH cord-349042-u9svz7pf 53 7 strand strand NN cord-349042-u9svz7pf 53 8 synthesis synthesis NN cord-349042-u9svz7pf 53 9 is be VBZ cord-349042-u9svz7pf 53 10 primed prime VBN cord-349042-u9svz7pf 53 11 with with IN cord-349042-u9svz7pf 53 12 the the DT cord-349042-u9svz7pf 53 13 oligo(dT)-T7 oligo(dt)-t7 JJ cord-349042-u9svz7pf 53 14 primer primer NN cord-349042-u9svz7pf 53 15 , , , cord-349042-u9svz7pf 53 16 which which WDT cord-349042-u9svz7pf 53 17 binds bind VBZ cord-349042-u9svz7pf 53 18 to to IN cord-349042-u9svz7pf 53 19 the the DT cord-349042-u9svz7pf 53 20 poly(A poly(a JJ cord-349042-u9svz7pf 53 21 ) ) -RRB- cord-349042-u9svz7pf 53 22 tail tail NN cord-349042-u9svz7pf 53 23 of of IN cord-349042-u9svz7pf 53 24 the the DT cord-349042-u9svz7pf 53 25 cDNA cdna NN cord-349042-u9svz7pf 53 26 created create VBN cord-349042-u9svz7pf 53 27 during during IN cord-349042-u9svz7pf 53 28 first first JJ cord-349042-u9svz7pf 53 29 - - HYPH cord-349042-u9svz7pf 53 30 strand strand NN cord-349042-u9svz7pf 53 31 synthesis synthesis NN cord-349042-u9svz7pf 53 32 by by IN cord-349042-u9svz7pf 53 33 DNA dna NN cord-349042-u9svz7pf 53 34 polymerase polymerase NN cord-349042-u9svz7pf 53 35 . . . cord-349042-u9svz7pf 54 1 Finally finally RB cord-349042-u9svz7pf 54 2 , , , cord-349042-u9svz7pf 54 3 RNA RNA NNP cord-349042-u9svz7pf 54 4 is be VBZ cord-349042-u9svz7pf 54 5 again again RB cord-349042-u9svz7pf 54 6 linearly linearly RB cord-349042-u9svz7pf 54 7 amplified amplify VBN cord-349042-u9svz7pf 54 8 through through IN cord-349042-u9svz7pf 54 9 the the DT cord-349042-u9svz7pf 54 10 enzymatic enzymatic JJ cord-349042-u9svz7pf 54 11 activity activity NN cord-349042-u9svz7pf 54 12 of of IN cord-349042-u9svz7pf 54 13 T7 T7 NNP cord-349042-u9svz7pf 54 14 RNA RNA NNP cord-349042-u9svz7pf 54 15 polymerase polymerase NN cord-349042-u9svz7pf 54 16 acting act VBG cord-349042-u9svz7pf 54 17 on on IN cord-349042-u9svz7pf 54 18 its -PRON- PRP$ cord-349042-u9svz7pf 54 19 promoter promoter NN cord-349042-u9svz7pf 54 20 that that WDT cord-349042-u9svz7pf 54 21 is be VBZ cord-349042-u9svz7pf 54 22 incorporated incorporate VBN cord-349042-u9svz7pf 54 23 into into IN cord-349042-u9svz7pf 54 24 the the DT cord-349042-u9svz7pf 54 25 double double RB cord-349042-u9svz7pf 54 26 - - HYPH cord-349042-u9svz7pf 54 27 stranded strand VBN cord-349042-u9svz7pf 54 28 cDNA cdna NN cord-349042-u9svz7pf 54 29 2,3 2,3 CD cord-349042-u9svz7pf 54 30 . . . cord-349042-u9svz7pf 55 1 Credit credit NN cord-349042-u9svz7pf 55 2 : : : cord-349042-u9svz7pf 56 1 Marina Marina NNP cord-349042-u9svz7pf 56 2 Spence Spence NNP cord-349042-u9svz7pf 56 3 / / SYM cord-349042-u9svz7pf 56 4 Springer Springer NNP cord-349042-u9svz7pf 56 5 Nature Nature NNP cord-349042-u9svz7pf 56 6 . . . cord-349042-u9svz7pf 57 1 After after IN cord-349042-u9svz7pf 57 2 two two CD cord-349042-u9svz7pf 57 3 rounds round NNS cord-349042-u9svz7pf 57 4 of of IN cord-349042-u9svz7pf 57 5 linear linear JJ cord-349042-u9svz7pf 57 6 aRNA arna NN cord-349042-u9svz7pf 57 7 amplification amplification NN cord-349042-u9svz7pf 57 8 from from IN cord-349042-u9svz7pf 57 9 isolated isolate VBN cord-349042-u9svz7pf 57 10 single single JJ cord-349042-u9svz7pf 57 11 cells cell NNS cord-349042-u9svz7pf 57 12 , , , cord-349042-u9svz7pf 57 13 an an DT cord-349042-u9svz7pf 57 14 Illumina Illumina NNP cord-349042-u9svz7pf 57 15 TruSeq TruSeq NNP cord-349042-u9svz7pf 57 16 library library NN cord-349042-u9svz7pf 57 17 is be VBZ cord-349042-u9svz7pf 57 18 generated generate VBN cord-349042-u9svz7pf 57 19 as as IN cord-349042-u9svz7pf 57 20 outlined outline VBN cord-349042-u9svz7pf 57 21 . . . cord-349042-u9svz7pf 58 1 Because because IN cord-349042-u9svz7pf 58 2 of of IN cord-349042-u9svz7pf 58 3 the the DT cord-349042-u9svz7pf 58 4 short short JJ cord-349042-u9svz7pf 58 5 length length NN cord-349042-u9svz7pf 58 6 of of IN cord-349042-u9svz7pf 58 7 the the DT cord-349042-u9svz7pf 58 8 aRNA arna NN cord-349042-u9svz7pf 58 9 amplified amplify VBN cord-349042-u9svz7pf 58 10 by by IN cord-349042-u9svz7pf 58 11 the the DT cord-349042-u9svz7pf 58 12 procedure procedure NN cord-349042-u9svz7pf 58 13 , , , cord-349042-u9svz7pf 58 14 the the DT cord-349042-u9svz7pf 58 15 step step NN cord-349042-u9svz7pf 58 16 that that WDT cord-349042-u9svz7pf 58 17 breaks break VBZ cord-349042-u9svz7pf 58 18 long long JJ cord-349042-u9svz7pf 58 19 RNA RNA NNP cord-349042-u9svz7pf 58 20 into into IN cord-349042-u9svz7pf 58 21 smaller small JJR cord-349042-u9svz7pf 58 22 parts part NNS cord-349042-u9svz7pf 58 23 in in IN cord-349042-u9svz7pf 58 24 the the DT cord-349042-u9svz7pf 58 25 original original JJ cord-349042-u9svz7pf 58 26 Illumina Illumina NNP cord-349042-u9svz7pf 58 27 library library NN cord-349042-u9svz7pf 58 28 protocol protocol NN cord-349042-u9svz7pf 58 29 can can MD cord-349042-u9svz7pf 58 30 be be VB cord-349042-u9svz7pf 58 31 omitted omit VBN cord-349042-u9svz7pf 58 32 . . . cord-349042-u9svz7pf 59 1 For for IN cord-349042-u9svz7pf 59 2 strand strand NN cord-349042-u9svz7pf 59 3 specificity specificity NN cord-349042-u9svz7pf 59 4 , , , cord-349042-u9svz7pf 59 5 deoxyuridine deoxyuridine NN cord-349042-u9svz7pf 59 6 triphosphate triphosphate NN cord-349042-u9svz7pf 59 7 is be VBZ cord-349042-u9svz7pf 59 8 incorporated incorporate VBN cord-349042-u9svz7pf 59 9 into into IN cord-349042-u9svz7pf 59 10 the the DT cord-349042-u9svz7pf 59 11 second second JJ cord-349042-u9svz7pf 59 12 - - HYPH cord-349042-u9svz7pf 59 13 strand strand NN cord-349042-u9svz7pf 59 14 cDNA cdna NN cord-349042-u9svz7pf 59 15 . . . cord-349042-u9svz7pf 60 1 After after IN cord-349042-u9svz7pf 60 2 sequencing sequence VBG cord-349042-u9svz7pf 60 3 adaptor adaptor NN cord-349042-u9svz7pf 60 4 ligation ligation NN cord-349042-u9svz7pf 60 5 , , , cord-349042-u9svz7pf 60 6 the the DT cord-349042-u9svz7pf 60 7 cDNA cdna NN cord-349042-u9svz7pf 60 8 fragments fragment NNS cord-349042-u9svz7pf 60 9 with with IN cord-349042-u9svz7pf 60 10 adaptors adaptor NNS cord-349042-u9svz7pf 60 11 at at IN cord-349042-u9svz7pf 60 12 both both DT cord-349042-u9svz7pf 60 13 end end NN cord-349042-u9svz7pf 60 14 are be VBP cord-349042-u9svz7pf 60 15 PCR PCR NNP cord-349042-u9svz7pf 60 16 amplified amplify VBN cord-349042-u9svz7pf 60 17 , , , cord-349042-u9svz7pf 60 18 and and CC cord-349042-u9svz7pf 60 19 ready ready JJ cord-349042-u9svz7pf 60 20 for for IN cord-349042-u9svz7pf 60 21 sequencing sequence VBG cord-349042-u9svz7pf 60 22 7 7 CD cord-349042-u9svz7pf 60 23 , , , cord-349042-u9svz7pf 60 24 8 8 CD cord-349042-u9svz7pf 60 25 . . . cord-349042-u9svz7pf 61 1 ( ( -LRB- cord-349042-u9svz7pf 61 2 b b LS cord-349042-u9svz7pf 61 3 ) ) -RRB- cord-349042-u9svz7pf 61 4 RNA rna NN cord-349042-u9svz7pf 61 5 - - HYPH cord-349042-u9svz7pf 61 6 binding bind VBG cord-349042-u9svz7pf 61 7 protein protein NN cord-349042-u9svz7pf 61 8 ( ( -LRB- cord-349042-u9svz7pf 61 9 RBP)/RNA rbp)/rna IN cord-349042-u9svz7pf 61 10 cargo cargo NN cord-349042-u9svz7pf 61 11 detection detection NN cord-349042-u9svz7pf 61 12 ( ( -LRB- cord-349042-u9svz7pf 61 13 APRA APRA NNP cord-349042-u9svz7pf 61 14 ) ) -RRB- cord-349042-u9svz7pf 61 15 . . . cord-349042-u9svz7pf 62 1 An an DT cord-349042-u9svz7pf 62 2 antibody antibody NN cord-349042-u9svz7pf 62 3 ( ( -LRB- cord-349042-u9svz7pf 62 4 Ab ab NN cord-349042-u9svz7pf 62 5 ) ) -RRB- cord-349042-u9svz7pf 62 6 to to IN cord-349042-u9svz7pf 62 7 RBP RBP NNP cord-349042-u9svz7pf 62 8 is be VBZ cord-349042-u9svz7pf 62 9 conjugated conjugate VBN cord-349042-u9svz7pf 62 10 to to IN cord-349042-u9svz7pf 62 11 an an DT cord-349042-u9svz7pf 62 12 oligonucleotide oligonucleotide NN cord-349042-u9svz7pf 62 13 and and CC cord-349042-u9svz7pf 62 14 applied apply VBN cord-349042-u9svz7pf 62 15 to to IN cord-349042-u9svz7pf 62 16 fixed fix VBN cord-349042-u9svz7pf 62 17 cells cell NNS cord-349042-u9svz7pf 62 18 . . . cord-349042-u9svz7pf 63 1 After after IN cord-349042-u9svz7pf 63 2 antibody antibody NN cord-349042-u9svz7pf 63 3 - - HYPH cord-349042-u9svz7pf 63 4 RBP rbp NN cord-349042-u9svz7pf 63 5 binding binding NN cord-349042-u9svz7pf 63 6 , , , cord-349042-u9svz7pf 63 7 the the DT cord-349042-u9svz7pf 63 8 oligonucleotide oligonucleotide NN cord-349042-u9svz7pf 63 9 ( ( -LRB- cord-349042-u9svz7pf 63 10 containing contain VBG cord-349042-u9svz7pf 63 11 the the DT cord-349042-u9svz7pf 63 12 T7 T7 NNP cord-349042-u9svz7pf 63 13 promoter promoter NN cord-349042-u9svz7pf 63 14 sequence sequence NN cord-349042-u9svz7pf 63 15 ) ) -RRB- cord-349042-u9svz7pf 63 16 is be VBZ cord-349042-u9svz7pf 63 17 positioned position VBN cord-349042-u9svz7pf 63 18 closely closely RB cord-349042-u9svz7pf 63 19 enough enough RB cord-349042-u9svz7pf 63 20 to to IN cord-349042-u9svz7pf 63 21 the the DT cord-349042-u9svz7pf 63 22 RNA RNA NNP cord-349042-u9svz7pf 63 23 to to IN cord-349042-u9svz7pf 63 24 prime prime JJ cord-349042-u9svz7pf 63 25 first first JJ cord-349042-u9svz7pf 63 26 - - HYPH cord-349042-u9svz7pf 63 27 strand strand NN cord-349042-u9svz7pf 63 28 cDNA cdna NN cord-349042-u9svz7pf 63 29 synthesis synthesis NN cord-349042-u9svz7pf 63 30 in in IN cord-349042-u9svz7pf 63 31 situ situ NN cord-349042-u9svz7pf 63 32 . . . cord-349042-u9svz7pf 64 1 After after IN cord-349042-u9svz7pf 64 2 second second JJ cord-349042-u9svz7pf 64 3 - - HYPH cord-349042-u9svz7pf 64 4 strand strand NN cord-349042-u9svz7pf 64 5 synthesis synthesis NN cord-349042-u9svz7pf 64 6 in in IN cord-349042-u9svz7pf 64 7 vitro vitro FW cord-349042-u9svz7pf 64 8 , , , cord-349042-u9svz7pf 64 9 the the DT cord-349042-u9svz7pf 64 10 antibody antibody NN cord-349042-u9svz7pf 64 11 is be VBZ cord-349042-u9svz7pf 64 12 removed remove VBN cord-349042-u9svz7pf 64 13 by by IN cord-349042-u9svz7pf 64 14 restriction restriction NN cord-349042-u9svz7pf 64 15 enzyme enzyme NN cord-349042-u9svz7pf 64 16 cleavage cleavage NN cord-349042-u9svz7pf 64 17 and and CC cord-349042-u9svz7pf 64 18 the the DT cord-349042-u9svz7pf 64 19 aRNA arna NN cord-349042-u9svz7pf 64 20 is be VBZ cord-349042-u9svz7pf 64 21 linearly linearly RB cord-349042-u9svz7pf 64 22 amplified amplify VBN cord-349042-u9svz7pf 64 23 by by IN cord-349042-u9svz7pf 64 24 in in IN cord-349042-u9svz7pf 64 25 vitro vitro FW cord-349042-u9svz7pf 64 26 transcription transcription NN cord-349042-u9svz7pf 64 27 using use VBG cord-349042-u9svz7pf 64 28 the the DT cord-349042-u9svz7pf 64 29 T7 T7 NNP cord-349042-u9svz7pf 64 30 promoter promoter NN cord-349042-u9svz7pf 64 31 incorporated incorporate VBN cord-349042-u9svz7pf 64 32 in in IN cord-349042-u9svz7pf 64 33 the the DT cord-349042-u9svz7pf 64 34 cDNA cdna NN cord-349042-u9svz7pf 64 35 . . . cord-349042-u9svz7pf 65 1 The the DT cord-349042-u9svz7pf 65 2 aRNA arna NN cord-349042-u9svz7pf 65 3 product product NN cord-349042-u9svz7pf 65 4 is be VBZ cord-349042-u9svz7pf 65 5 suitable suitable JJ cord-349042-u9svz7pf 65 6 for for IN cord-349042-u9svz7pf 65 7 PCR PCR NNP cord-349042-u9svz7pf 65 8 , , , cord-349042-u9svz7pf 65 9 microarray microarray NN cord-349042-u9svz7pf 65 10 , , , cord-349042-u9svz7pf 65 11 and and CC cord-349042-u9svz7pf 65 12 next next JJ cord-349042-u9svz7pf 65 13 - - HYPH cord-349042-u9svz7pf 65 14 generation generation NN cord-349042-u9svz7pf 65 15 sequencing sequencing NN cord-349042-u9svz7pf 65 16 analysis analysis NN cord-349042-u9svz7pf 65 17 43 43 CD cord-349042-u9svz7pf 65 18 . . . cord-349042-u9svz7pf 66 1 ( ( -LRB- cord-349042-u9svz7pf 66 2 c c NN cord-349042-u9svz7pf 66 3 ) ) -RRB- cord-349042-u9svz7pf 66 4 Protein protein NN cord-349042-u9svz7pf 66 5 detection detection NN cord-349042-u9svz7pf 66 6 ( ( -LRB- cord-349042-u9svz7pf 66 7 IDAT IDAT NNP cord-349042-u9svz7pf 66 8 ) ) -RRB- cord-349042-u9svz7pf 66 9 . . . cord-349042-u9svz7pf 67 1 A a DT cord-349042-u9svz7pf 67 2 detection detection NN cord-349042-u9svz7pf 67 3 antibody antibody NN cord-349042-u9svz7pf 67 4 is be VBZ cord-349042-u9svz7pf 67 5 first first RB cord-349042-u9svz7pf 67 6 generated generate VBN cord-349042-u9svz7pf 67 7 by by IN cord-349042-u9svz7pf 67 8 conjugation conjugation NN cord-349042-u9svz7pf 67 9 of of IN cord-349042-u9svz7pf 67 10 a a DT cord-349042-u9svz7pf 67 11 target target NN cord-349042-u9svz7pf 67 12 - - HYPH cord-349042-u9svz7pf 67 13 protein protein NN cord-349042-u9svz7pf 67 14 - - HYPH cord-349042-u9svz7pf 67 15 specific specific JJ cord-349042-u9svz7pf 67 16 antibody antibody NN cord-349042-u9svz7pf 67 17 to to IN cord-349042-u9svz7pf 67 18 a a DT cord-349042-u9svz7pf 67 19 double double RB cord-349042-u9svz7pf 67 20 - - HYPH cord-349042-u9svz7pf 67 21 stranded stranded JJ cord-349042-u9svz7pf 67 22 ( ( -LRB- cord-349042-u9svz7pf 67 23 ds ds NNP cord-349042-u9svz7pf 67 24 ) ) -RRB- cord-349042-u9svz7pf 67 25 oligonucleotide oligonucleotide NN cord-349042-u9svz7pf 67 26 containing contain VBG cord-349042-u9svz7pf 67 27 T7 t7 NN cord-349042-u9svz7pf 67 28 promoter promoter NN cord-349042-u9svz7pf 67 29 . . . cord-349042-u9svz7pf 68 1 In in IN cord-349042-u9svz7pf 68 2 a a DT cord-349042-u9svz7pf 68 3 96-well 96-well CD cord-349042-u9svz7pf 68 4 plate plate NN cord-349042-u9svz7pf 68 5 , , , cord-349042-u9svz7pf 68 6 a a DT cord-349042-u9svz7pf 68 7 capture capture NN cord-349042-u9svz7pf 68 8 antibody antibody NN cord-349042-u9svz7pf 68 9 then then RB cord-349042-u9svz7pf 68 10 binds bind VBZ cord-349042-u9svz7pf 68 11 to to IN cord-349042-u9svz7pf 68 12 the the DT cord-349042-u9svz7pf 68 13 antigen antigen NN cord-349042-u9svz7pf 68 14 ( ( -LRB- cord-349042-u9svz7pf 68 15 Ag Ag NNP cord-349042-u9svz7pf 68 16 ) ) -RRB- cord-349042-u9svz7pf 68 17 of of IN cord-349042-u9svz7pf 68 18 interest interest NN cord-349042-u9svz7pf 68 19 from from IN cord-349042-u9svz7pf 68 20 a a DT cord-349042-u9svz7pf 68 21 sample sample NN cord-349042-u9svz7pf 68 22 . . . cord-349042-u9svz7pf 69 1 After after IN cord-349042-u9svz7pf 69 2 the the DT cord-349042-u9svz7pf 69 3 addition addition NN cord-349042-u9svz7pf 69 4 of of IN cord-349042-u9svz7pf 69 5 detection detection NN cord-349042-u9svz7pf 69 6 antibody antibody NN cord-349042-u9svz7pf 69 7 to to IN cord-349042-u9svz7pf 69 8 the the DT cord-349042-u9svz7pf 69 9 sample sample NN cord-349042-u9svz7pf 69 10 , , , cord-349042-u9svz7pf 69 11 RNA RNA NNP cord-349042-u9svz7pf 69 12 is be VBZ cord-349042-u9svz7pf 69 13 linearly linearly RB cord-349042-u9svz7pf 69 14 amplified amplify VBN cord-349042-u9svz7pf 69 15 by by IN cord-349042-u9svz7pf 69 16 T7 T7 NNP cord-349042-u9svz7pf 69 17 RNA RNA NNP cord-349042-u9svz7pf 69 18 polymerase polymerase NN cord-349042-u9svz7pf 69 19 from from IN cord-349042-u9svz7pf 69 20 the the DT cord-349042-u9svz7pf 69 21 double double RB cord-349042-u9svz7pf 69 22 - - HYPH cord-349042-u9svz7pf 69 23 stranded strand VBN cord-349042-u9svz7pf 69 24 oligonucleotide oligonucleotide NN cord-349042-u9svz7pf 69 25 template template NN cord-349042-u9svz7pf 69 26 incorporating incorporate VBG cord-349042-u9svz7pf 69 27 the the DT cord-349042-u9svz7pf 69 28 T7 T7 NNP cord-349042-u9svz7pf 69 29 promoter promoter NN cord-349042-u9svz7pf 69 30 . . . cord-349042-u9svz7pf 70 1 The the DT cord-349042-u9svz7pf 70 2 amount amount NN cord-349042-u9svz7pf 70 3 of of IN cord-349042-u9svz7pf 70 4 RNA RNA NNP cord-349042-u9svz7pf 70 5 product product NN cord-349042-u9svz7pf 70 6 is be VBZ cord-349042-u9svz7pf 70 7 indicative indicative JJ cord-349042-u9svz7pf 70 8 of of IN cord-349042-u9svz7pf 70 9 the the DT cord-349042-u9svz7pf 70 10 original original JJ cord-349042-u9svz7pf 70 11 amount amount NN cord-349042-u9svz7pf 70 12 of of IN cord-349042-u9svz7pf 70 13 antigen antigen NN cord-349042-u9svz7pf 70 14 in in IN cord-349042-u9svz7pf 70 15 the the DT cord-349042-u9svz7pf 70 16 sample sample NN cord-349042-u9svz7pf 70 17 and and CC cord-349042-u9svz7pf 70 18 can can MD cord-349042-u9svz7pf 70 19 be be VB cord-349042-u9svz7pf 70 20 used use VBN cord-349042-u9svz7pf 70 21 for for IN cord-349042-u9svz7pf 70 22 fluorimeter fluorimeter NN cord-349042-u9svz7pf 70 23 detection detection NN cord-349042-u9svz7pf 70 24 , , , cord-349042-u9svz7pf 70 25 PCR PCR NNP cord-349042-u9svz7pf 70 26 . . . cord-349042-u9svz7pf 70 27 or or CC cord-349042-u9svz7pf 70 28 sequencing sequence VBG cord-349042-u9svz7pf 70 29 45 45 CD cord-349042-u9svz7pf 70 30 . . . cord-349042-u9svz7pf 71 1 ( ( -LRB- cord-349042-u9svz7pf 71 2 d d LS cord-349042-u9svz7pf 71 3 ) ) -RRB- cord-349042-u9svz7pf 71 4 Whole whole JJ cord-349042-u9svz7pf 71 5 - - HYPH cord-349042-u9svz7pf 71 6 genome genome NN cord-349042-u9svz7pf 71 7 DNA dna NN cord-349042-u9svz7pf 71 8 amplification amplification NN cord-349042-u9svz7pf 71 9 ( ( -LRB- cord-349042-u9svz7pf 71 10 LIANTI LIANTI NNP cord-349042-u9svz7pf 71 11 ) ) -RRB- cord-349042-u9svz7pf 71 12 . . . cord-349042-u9svz7pf 72 1 A a DT cord-349042-u9svz7pf 72 2 LIANTI LIANTI NNP cord-349042-u9svz7pf 72 3 transposon transposon NN cord-349042-u9svz7pf 72 4 is be VBZ cord-349042-u9svz7pf 72 5 first first RB cord-349042-u9svz7pf 72 6 created create VBN cord-349042-u9svz7pf 72 7 by by IN cord-349042-u9svz7pf 72 8 joining join VBG cord-349042-u9svz7pf 72 9 of of IN cord-349042-u9svz7pf 72 10 the the DT cord-349042-u9svz7pf 72 11 T7 T7 NNP cord-349042-u9svz7pf 72 12 promoter promoter NN cord-349042-u9svz7pf 72 13 site site NN cord-349042-u9svz7pf 72 14 to to IN cord-349042-u9svz7pf 72 15 a a DT cord-349042-u9svz7pf 72 16 transposase transposase NN cord-349042-u9svz7pf 72 17 - - HYPH cord-349042-u9svz7pf 72 18 binding bind VBG cord-349042-u9svz7pf 72 19 site site NN cord-349042-u9svz7pf 72 20 . . . cord-349042-u9svz7pf 73 1 This this DT cord-349042-u9svz7pf 73 2 transposon transposon NN cord-349042-u9svz7pf 73 3 is be VBZ cord-349042-u9svz7pf 73 4 then then RB cord-349042-u9svz7pf 73 5 mixed mix VBN cord-349042-u9svz7pf 73 6 with with IN cord-349042-u9svz7pf 73 7 transposase transposase NNP cord-349042-u9svz7pf 73 8 to to TO cord-349042-u9svz7pf 73 9 generate generate VB cord-349042-u9svz7pf 73 10 the the DT cord-349042-u9svz7pf 73 11 LIANTI LIANTI NNP cord-349042-u9svz7pf 73 12 transposome transposome NN cord-349042-u9svz7pf 73 13 . . . cord-349042-u9svz7pf 74 1 After after IN cord-349042-u9svz7pf 74 2 the the DT cord-349042-u9svz7pf 74 3 LIANTI LIANTI NNP cord-349042-u9svz7pf 74 4 transposome transposome NN cord-349042-u9svz7pf 74 5 is be VBZ cord-349042-u9svz7pf 74 6 mixed mix VBN cord-349042-u9svz7pf 74 7 with with IN cord-349042-u9svz7pf 74 8 DNA dna NN cord-349042-u9svz7pf 74 9 isolated isolate VBN cord-349042-u9svz7pf 74 10 from from IN cord-349042-u9svz7pf 74 11 single single JJ cord-349042-u9svz7pf 74 12 cells cell NNS cord-349042-u9svz7pf 74 13 , , , cord-349042-u9svz7pf 74 14 the the DT cord-349042-u9svz7pf 74 15 transposase transposase NNP cord-349042-u9svz7pf 74 16 mediates mediate VBZ cord-349042-u9svz7pf 74 17 random random JJ cord-349042-u9svz7pf 74 18 insertion insertion NN cord-349042-u9svz7pf 74 19 of of IN cord-349042-u9svz7pf 74 20 LIANTI LIANTI NNP cord-349042-u9svz7pf 74 21 transposon transposon NN cord-349042-u9svz7pf 74 22 into into IN cord-349042-u9svz7pf 74 23 the the DT cord-349042-u9svz7pf 74 24 DNA dna NN cord-349042-u9svz7pf 74 25 and and CC cord-349042-u9svz7pf 74 26 subsequent subsequent JJ cord-349042-u9svz7pf 74 27 excision excision NN cord-349042-u9svz7pf 74 28 of of IN cord-349042-u9svz7pf 74 29 genomic genomic JJ cord-349042-u9svz7pf 74 30 DNA dna NN cord-349042-u9svz7pf 74 31 , , , cord-349042-u9svz7pf 74 32 which which WDT cord-349042-u9svz7pf 74 33 is be VBZ cord-349042-u9svz7pf 74 34 followed follow VBN cord-349042-u9svz7pf 74 35 by by IN cord-349042-u9svz7pf 74 36 DNA dna NN cord-349042-u9svz7pf 74 37 polymerase polymerase NN cord-349042-u9svz7pf 74 38 gap gap NN cord-349042-u9svz7pf 74 39 extension extension NN cord-349042-u9svz7pf 74 40 . . . cord-349042-u9svz7pf 75 1 After after IN cord-349042-u9svz7pf 75 2 the the DT cord-349042-u9svz7pf 75 3 addition addition NN cord-349042-u9svz7pf 75 4 of of IN cord-349042-u9svz7pf 75 5 T7 T7 NNP cord-349042-u9svz7pf 75 6 RNA RNA NNP cord-349042-u9svz7pf 75 7 polymerase polymerase NN cord-349042-u9svz7pf 75 8 , , , cord-349042-u9svz7pf 75 9 single single RB cord-349042-u9svz7pf 75 10 - - HYPH cord-349042-u9svz7pf 75 11 stranded strand VBN cord-349042-u9svz7pf 75 12 aRNA arna NN cord-349042-u9svz7pf 75 13 is be VBZ cord-349042-u9svz7pf 75 14 generated generate VBN cord-349042-u9svz7pf 75 15 that that WDT cord-349042-u9svz7pf 75 16 is be VBZ cord-349042-u9svz7pf 75 17 capable capable JJ cord-349042-u9svz7pf 75 18 of of IN cord-349042-u9svz7pf 75 19 self self NN cord-349042-u9svz7pf 75 20 - - HYPH cord-349042-u9svz7pf 75 21 priming priming NN cord-349042-u9svz7pf 75 22 on on IN cord-349042-u9svz7pf 75 23 the the DT cord-349042-u9svz7pf 75 24 3′ 3′ CD cord-349042-u9svz7pf 75 25 end end NN cord-349042-u9svz7pf 75 26 . . . cord-349042-u9svz7pf 76 1 After after IN cord-349042-u9svz7pf 76 2 reverse reverse JJ cord-349042-u9svz7pf 76 3 transcription transcription NN cord-349042-u9svz7pf 76 4 , , , cord-349042-u9svz7pf 76 5 RNase rnase NN cord-349042-u9svz7pf 76 6 treatment treatment NN cord-349042-u9svz7pf 76 7 , , , cord-349042-u9svz7pf 76 8 and and CC cord-349042-u9svz7pf 76 9 second second JJ cord-349042-u9svz7pf 76 10 - - HYPH cord-349042-u9svz7pf 76 11 strand strand NN cord-349042-u9svz7pf 76 12 synthesis synthesis NN cord-349042-u9svz7pf 76 13 , , , cord-349042-u9svz7pf 76 14 double double RB cord-349042-u9svz7pf 76 15 - - HYPH cord-349042-u9svz7pf 76 16 stranded strand VBN cord-349042-u9svz7pf 76 17 LIANTI LIANTI NNP cord-349042-u9svz7pf 76 18 amplicons amplicons IN cord-349042-u9svz7pf 76 19 tagged tag VBN cord-349042-u9svz7pf 76 20 with with IN cord-349042-u9svz7pf 76 21 unique unique JJ cord-349042-u9svz7pf 76 22 molecular molecular JJ cord-349042-u9svz7pf 76 23 barcodes barcode NNS cord-349042-u9svz7pf 76 24 are be VBP cord-349042-u9svz7pf 76 25 formed form VBN cord-349042-u9svz7pf 76 26 and and CC cord-349042-u9svz7pf 76 27 ready ready JJ cord-349042-u9svz7pf 76 28 for for IN cord-349042-u9svz7pf 76 29 DNA dna NN cord-349042-u9svz7pf 76 30 library library NN cord-349042-u9svz7pf 76 31 preparation preparation NN cord-349042-u9svz7pf 76 32 and and CC cord-349042-u9svz7pf 76 33 next next JJ cord-349042-u9svz7pf 76 34 - - HYPH cord-349042-u9svz7pf 76 35 generation generation NN cord-349042-u9svz7pf 76 36 sequencing sequence VBG cord-349042-u9svz7pf 76 37 11 11 CD cord-349042-u9svz7pf 76 38 voltage voltage NN cord-349042-u9svz7pf 76 39 - - HYPH cord-349042-u9svz7pf 76 40 clamp clamp NN cord-349042-u9svz7pf 76 41 techniques technique NNS cord-349042-u9svz7pf 76 42 . . . cord-349042-u9svz7pf 77 1 Using use VBG cord-349042-u9svz7pf 77 2 RNA RNA NNP cord-349042-u9svz7pf 77 3 deep deep JJ cord-349042-u9svz7pf 77 4 sequencing sequencing NN cord-349042-u9svz7pf 77 5 , , , cord-349042-u9svz7pf 77 6 the the DT cord-349042-u9svz7pf 77 7 same same JJ cord-349042-u9svz7pf 77 8 group group NN cord-349042-u9svz7pf 77 9 also also RB cord-349042-u9svz7pf 77 10 detected detect VBD cord-349042-u9svz7pf 77 11 more more JJR cord-349042-u9svz7pf 77 12 than than IN cord-349042-u9svz7pf 77 13 12,000 12,000 CD cord-349042-u9svz7pf 77 14 expressed express VBN cord-349042-u9svz7pf 77 15 genes gene NNS cord-349042-u9svz7pf 77 16 in in IN cord-349042-u9svz7pf 77 17 primary primary JJ cord-349042-u9svz7pf 77 18 cells cell NNS cord-349042-u9svz7pf 77 19 from from IN cord-349042-u9svz7pf 77 20 live live JJ cord-349042-u9svz7pf 77 21 neurosurgically neurosurgically RB cord-349042-u9svz7pf 77 22 resected resect VBN cord-349042-u9svz7pf 77 23 adult adult NN cord-349042-u9svz7pf 77 24 human human JJ cord-349042-u9svz7pf 77 25 brains brain NNS cord-349042-u9svz7pf 77 26 , , , cord-349042-u9svz7pf 77 27 uncovering uncover VBG cord-349042-u9svz7pf 77 28 cell cell NN cord-349042-u9svz7pf 77 29 - - HYPH cord-349042-u9svz7pf 77 30 type type NN cord-349042-u9svz7pf 77 31 - - HYPH cord-349042-u9svz7pf 77 32 specific specific JJ cord-349042-u9svz7pf 77 33 and and CC cord-349042-u9svz7pf 77 34 patient patient JJ cord-349042-u9svz7pf 77 35 - - HYPH cord-349042-u9svz7pf 77 36 specific specific JJ cord-349042-u9svz7pf 77 37 transcriptional transcriptional JJ cord-349042-u9svz7pf 77 38 hierarchies hierarchy NNS cord-349042-u9svz7pf 77 39 7 7 CD cord-349042-u9svz7pf 77 40 . . . cord-349042-u9svz7pf 78 1 CEL CEL NNP cord-349042-u9svz7pf 78 2 - - HYPH cord-349042-u9svz7pf 78 3 seq seq NNP cord-349042-u9svz7pf 78 4 is be VBZ cord-349042-u9svz7pf 78 5 a a DT cord-349042-u9svz7pf 78 6 modified modify VBN cord-349042-u9svz7pf 78 7 aRNA arna NN cord-349042-u9svz7pf 78 8 method method NN cord-349042-u9svz7pf 78 9 that that WDT cord-349042-u9svz7pf 78 10 allows allow VBZ cord-349042-u9svz7pf 78 11 for for IN cord-349042-u9svz7pf 78 12 multiplexing multiplexing NN cord-349042-u9svz7pf 78 13 of of IN cord-349042-u9svz7pf 78 14 samples sample NNS cord-349042-u9svz7pf 78 15 and and CC cord-349042-u9svz7pf 78 16 high high JJ cord-349042-u9svz7pf 78 17 - - HYPH cord-349042-u9svz7pf 78 18 throughput throughput NN cord-349042-u9svz7pf 78 19 , , , cord-349042-u9svz7pf 78 20 next next JJ cord-349042-u9svz7pf 78 21 - - HYPH cord-349042-u9svz7pf 78 22 generation generation NN cord-349042-u9svz7pf 78 23 sequencing sequencing NN cord-349042-u9svz7pf 78 24 9 9 CD cord-349042-u9svz7pf 78 25 . . . cord-349042-u9svz7pf 79 1 Multiplexing multiplexe VBG cord-349042-u9svz7pf 79 2 is be VBZ cord-349042-u9svz7pf 79 3 facilitated facilitate VBN cord-349042-u9svz7pf 79 4 by by IN cord-349042-u9svz7pf 79 5 alteration alteration NN cord-349042-u9svz7pf 79 6 of of IN cord-349042-u9svz7pf 79 7 the the DT cord-349042-u9svz7pf 79 8 original original JJ cord-349042-u9svz7pf 79 9 aRNA arna NN cord-349042-u9svz7pf 79 10 primer primer NN cord-349042-u9svz7pf 79 11 so so IN cord-349042-u9svz7pf 79 12 that that IN cord-349042-u9svz7pf 79 13 it -PRON- PRP cord-349042-u9svz7pf 79 14 contains contain VBZ cord-349042-u9svz7pf 79 15 both both CC cord-349042-u9svz7pf 79 16 a a DT cord-349042-u9svz7pf 79 17 unique unique JJ cord-349042-u9svz7pf 79 18 barcode barcode NN cord-349042-u9svz7pf 79 19 and and CC cord-349042-u9svz7pf 79 20 a a DT cord-349042-u9svz7pf 79 21 5′ 5′ CD cord-349042-u9svz7pf 79 22 Illumina Illumina NNP cord-349042-u9svz7pf 79 23 sequencing sequencing NN cord-349042-u9svz7pf 79 24 adaptor adaptor NN cord-349042-u9svz7pf 79 25 between between IN cord-349042-u9svz7pf 79 26 the the DT cord-349042-u9svz7pf 79 27 anchored anchored JJ cord-349042-u9svz7pf 79 28 poly(T poly(T NNP cord-349042-u9svz7pf 79 29 ) ) -RRB- cord-349042-u9svz7pf 79 30 and and CC cord-349042-u9svz7pf 79 31 T7 t7 NN cord-349042-u9svz7pf 79 32 promoter promoter NN cord-349042-u9svz7pf 79 33 sequences sequence NNS cord-349042-u9svz7pf 79 34 . . . cord-349042-u9svz7pf 80 1 When when WRB cord-349042-u9svz7pf 80 2 aRNA aRNA NNP cord-349042-u9svz7pf 80 3 is be VBZ cord-349042-u9svz7pf 80 4 made make VBN cord-349042-u9svz7pf 80 5 via via IN cord-349042-u9svz7pf 80 6 this this DT cord-349042-u9svz7pf 80 7 procedure procedure NN cord-349042-u9svz7pf 80 8 , , , cord-349042-u9svz7pf 80 9 it -PRON- PRP cord-349042-u9svz7pf 80 10 contains contain VBZ cord-349042-u9svz7pf 80 11 a a DT cord-349042-u9svz7pf 80 12 barcode barcode NN cord-349042-u9svz7pf 80 13 and and CC cord-349042-u9svz7pf 80 14 a a DT cord-349042-u9svz7pf 80 15 5′ 5′ CD cord-349042-u9svz7pf 80 16 sequencing sequencing NN cord-349042-u9svz7pf 80 17 primer primer NN cord-349042-u9svz7pf 80 18 , , , cord-349042-u9svz7pf 80 19 which which WDT cord-349042-u9svz7pf 80 20 enables enable VBZ cord-349042-u9svz7pf 80 21 many many JJ cord-349042-u9svz7pf 80 22 cells cell NNS cord-349042-u9svz7pf 80 23 to to TO cord-349042-u9svz7pf 80 24 be be VB cord-349042-u9svz7pf 80 25 barcoded barcode VBN cord-349042-u9svz7pf 80 26 and and CC cord-349042-u9svz7pf 80 27 sequenced sequence VBN cord-349042-u9svz7pf 80 28 simultaneously simultaneously RB cord-349042-u9svz7pf 80 29 . . . cord-349042-u9svz7pf 81 1 The the DT cord-349042-u9svz7pf 81 2 Yanai Yanai NNP cord-349042-u9svz7pf 81 3 group group NN cord-349042-u9svz7pf 81 4 later later RB cord-349042-u9svz7pf 81 5 adapted adapt VBD cord-349042-u9svz7pf 81 6 CEL CEL NNP cord-349042-u9svz7pf 81 7 - - HYPH cord-349042-u9svz7pf 81 8 seq seq NN cord-349042-u9svz7pf 81 9 for for IN cord-349042-u9svz7pf 81 10 use use NN cord-349042-u9svz7pf 81 11 on on IN cord-349042-u9svz7pf 81 12 Fluidigm Fluidigm NNP cord-349042-u9svz7pf 81 13 's 's POS cord-349042-u9svz7pf 81 14 C1 c1 NN cord-349042-u9svz7pf 81 15 system system NN cord-349042-u9svz7pf 81 16 by by IN cord-349042-u9svz7pf 81 17 adding add VBG cord-349042-u9svz7pf 81 18 unique unique JJ cord-349042-u9svz7pf 81 19 molecular molecular JJ cord-349042-u9svz7pf 81 20 identifiers identifier NNS cord-349042-u9svz7pf 81 21 to to IN cord-349042-u9svz7pf 81 22 the the DT cord-349042-u9svz7pf 81 23 primer primer NN cord-349042-u9svz7pf 81 24 24 24 CD cord-349042-u9svz7pf 81 25 that that WDT cord-349042-u9svz7pf 81 26 allowed allow VBD cord-349042-u9svz7pf 81 27 RNA rna NN cord-349042-u9svz7pf 81 28 molecules molecule NNS cord-349042-u9svz7pf 81 29 to to TO cord-349042-u9svz7pf 81 30 be be VB cord-349042-u9svz7pf 81 31 counted count VBN cord-349042-u9svz7pf 81 32 . . . cord-349042-u9svz7pf 82 1 Later later RBR cord-349042-u9svz7pf 82 2 , , , cord-349042-u9svz7pf 82 3 Jaitin Jaitin NNP cord-349042-u9svz7pf 82 4 et et NNP cord-349042-u9svz7pf 82 5 al al NNP cord-349042-u9svz7pf 82 6 . . . cord-349042-u9svz7pf 83 1 17 17 CD cord-349042-u9svz7pf 83 2 also also RB cord-349042-u9svz7pf 83 3 reported report VBD cord-349042-u9svz7pf 83 4 the the DT cord-349042-u9svz7pf 83 5 same same JJ cord-349042-u9svz7pf 83 6 approach approach NN cord-349042-u9svz7pf 83 7 , , , cord-349042-u9svz7pf 83 8 which which WDT cord-349042-u9svz7pf 83 9 they -PRON- PRP cord-349042-u9svz7pf 83 10 renamed rename VBD cord-349042-u9svz7pf 83 11 massively massively RB cord-349042-u9svz7pf 83 12 parallel parallel JJ cord-349042-u9svz7pf 83 13 single single JJ cord-349042-u9svz7pf 83 14 - - HYPH cord-349042-u9svz7pf 83 15 cell cell NN cord-349042-u9svz7pf 83 16 RNA rna NN cord-349042-u9svz7pf 83 17 sequencing sequencing NN cord-349042-u9svz7pf 83 18 ( ( -LRB- cord-349042-u9svz7pf 83 19 MARS MARS NNP cord-349042-u9svz7pf 83 20 - - HYPH cord-349042-u9svz7pf 83 21 seq seq NNP cord-349042-u9svz7pf 83 22 ) ) -RRB- cord-349042-u9svz7pf 83 23 . . . cord-349042-u9svz7pf 84 1 In in IN cord-349042-u9svz7pf 84 2 an an DT cord-349042-u9svz7pf 84 3 elegant elegant JJ cord-349042-u9svz7pf 84 4 study study NN cord-349042-u9svz7pf 84 5 using use VBG cord-349042-u9svz7pf 84 6 CEL cel NN cord-349042-u9svz7pf 84 7 - - HYPH cord-349042-u9svz7pf 84 8 seq seq NNP cord-349042-u9svz7pf 84 9 , , , cord-349042-u9svz7pf 84 10 Hashimshony Hashimshony NNP cord-349042-u9svz7pf 84 11 et et NNP cord-349042-u9svz7pf 84 12 al al NNP cord-349042-u9svz7pf 84 13 . . . cord-349042-u9svz7pf 85 1 9 9 LS cord-349042-u9svz7pf 85 2 studied study VBD cord-349042-u9svz7pf 85 3 the the DT cord-349042-u9svz7pf 85 4 embryonic embryonic JJ cord-349042-u9svz7pf 85 5 development development NN cord-349042-u9svz7pf 85 6 of of IN cord-349042-u9svz7pf 85 7 Caenorhabditis Caenorhabditis NNP cord-349042-u9svz7pf 85 8 elegans elegan NNS cord-349042-u9svz7pf 85 9 and and CC cord-349042-u9svz7pf 85 10 reported report VBD cord-349042-u9svz7pf 85 11 differential differential JJ cord-349042-u9svz7pf 85 12 distribution distribution NN cord-349042-u9svz7pf 85 13 of of IN cord-349042-u9svz7pf 85 14 transcripts transcript NNS cord-349042-u9svz7pf 85 15 between between IN cord-349042-u9svz7pf 85 16 sister sister NN cord-349042-u9svz7pf 85 17 cells cell NNS cord-349042-u9svz7pf 85 18 as as RB cord-349042-u9svz7pf 85 19 early early RB cord-349042-u9svz7pf 85 20 as as IN cord-349042-u9svz7pf 85 21 the the DT cord-349042-u9svz7pf 85 22 two two CD cord-349042-u9svz7pf 85 23 - - HYPH cord-349042-u9svz7pf 85 24 cell cell NN cord-349042-u9svz7pf 85 25 - - HYPH cord-349042-u9svz7pf 85 26 stage stage NN cord-349042-u9svz7pf 85 27 embryo embryo NN cord-349042-u9svz7pf 85 28 , , , cord-349042-u9svz7pf 85 29 identifying identify VBG cord-349042-u9svz7pf 85 30 gene gene NN cord-349042-u9svz7pf 85 31 expression expression NN cord-349042-u9svz7pf 85 32 in in IN cord-349042-u9svz7pf 85 33 a a DT cord-349042-u9svz7pf 85 34 cell cell NN cord-349042-u9svz7pf 85 35 previously previously RB cord-349042-u9svz7pf 85 36 thought think VBN cord-349042-u9svz7pf 85 37 to to TO cord-349042-u9svz7pf 85 38 be be VB cord-349042-u9svz7pf 85 39 transcriptionally transcriptionally RB cord-349042-u9svz7pf 85 40 inert inert JJ cord-349042-u9svz7pf 85 41 . . . cord-349042-u9svz7pf 86 1 Jaitin Jaitin NNP cord-349042-u9svz7pf 86 2 et et NNP cord-349042-u9svz7pf 86 3 al al NNP cord-349042-u9svz7pf 86 4 . . . cord-349042-u9svz7pf 87 1 17 17 CD cord-349042-u9svz7pf 87 2 carried carry VBD cord-349042-u9svz7pf 87 3 out out RP cord-349042-u9svz7pf 87 4 multiplexing multiplexe VBG cord-349042-u9svz7pf 87 5 RNA RNA NNP cord-349042-u9svz7pf 87 6 - - HYPH cord-349042-u9svz7pf 87 7 seq seq NNP cord-349042-u9svz7pf 87 8 ( ( -LRB- cord-349042-u9svz7pf 87 9 MARSseq MARSseq NNP cord-349042-u9svz7pf 87 10 ) ) -RRB- cord-349042-u9svz7pf 87 11 to to TO cord-349042-u9svz7pf 87 12 sample sample VB cord-349042-u9svz7pf 87 13 thousands thousand NNS cord-349042-u9svz7pf 87 14 of of IN cord-349042-u9svz7pf 87 15 mouse mouse NN cord-349042-u9svz7pf 87 16 hematopoietic hematopoietic JJ cord-349042-u9svz7pf 87 17 cells cell NNS cord-349042-u9svz7pf 87 18 from from IN cord-349042-u9svz7pf 87 19 the the DT cord-349042-u9svz7pf 87 20 spleen spleen NN cord-349042-u9svz7pf 87 21 , , , cord-349042-u9svz7pf 87 22 and and CC cord-349042-u9svz7pf 87 23 uncovered uncovered JJ cord-349042-u9svz7pf 87 24 cell cell NN cord-349042-u9svz7pf 87 25 - - HYPH cord-349042-u9svz7pf 87 26 type type NN cord-349042-u9svz7pf 87 27 heterogeneity heterogeneity NN cord-349042-u9svz7pf 87 28 in in IN cord-349042-u9svz7pf 87 29 steadystate steadystate NN cord-349042-u9svz7pf 87 30 gene gene NN cord-349042-u9svz7pf 87 31 expression expression NN cord-349042-u9svz7pf 87 32 levels level NNS cord-349042-u9svz7pf 87 33 and and CC cord-349042-u9svz7pf 87 34 regulated regulate VBN cord-349042-u9svz7pf 87 35 changes change NNS cord-349042-u9svz7pf 87 36 in in IN cord-349042-u9svz7pf 87 37 RNA RNA NNP cord-349042-u9svz7pf 87 38 abundances abundance NNS cord-349042-u9svz7pf 87 39 after after IN cord-349042-u9svz7pf 87 40 pathogen pathogen NN cord-349042-u9svz7pf 87 41 activation activation NN cord-349042-u9svz7pf 87 42 . . . cord-349042-u9svz7pf 88 1 Most most RBS cord-349042-u9svz7pf 88 2 recently recently RB cord-349042-u9svz7pf 88 3 , , , cord-349042-u9svz7pf 88 4 inDrop inDrop NNP cord-349042-u9svz7pf 88 5 ( ( -LRB- cord-349042-u9svz7pf 88 6 ' ' `` cord-349042-u9svz7pf 88 7 indexing indexing NN cord-349042-u9svz7pf 88 8 droplets droplet NNS cord-349042-u9svz7pf 88 9 ' ' '' cord-349042-u9svz7pf 88 10 ) ) -RRB- cord-349042-u9svz7pf 89 1 sequencing sequencing NN cord-349042-u9svz7pf 89 2 technology technology NN cord-349042-u9svz7pf 89 3 was be VBD cord-349042-u9svz7pf 89 4 developed develop VBN cord-349042-u9svz7pf 89 5 by by IN cord-349042-u9svz7pf 89 6 Klein Klein NNP cord-349042-u9svz7pf 89 7 and and CC cord-349042-u9svz7pf 89 8 colleagues colleague NNS cord-349042-u9svz7pf 89 9 25 25 CD cord-349042-u9svz7pf 89 10 , , , cord-349042-u9svz7pf 89 11 26 26 CD cord-349042-u9svz7pf 89 12 . . . cord-349042-u9svz7pf 90 1 This this DT cord-349042-u9svz7pf 90 2 microfluidic microfluidic JJ cord-349042-u9svz7pf 90 3 approach approach NN cord-349042-u9svz7pf 90 4 encapsulates encapsulate VBZ cord-349042-u9svz7pf 90 5 single single JJ cord-349042-u9svz7pf 90 6 cells cell NNS cord-349042-u9svz7pf 90 7 in in IN cord-349042-u9svz7pf 90 8 reaction reaction NN cord-349042-u9svz7pf 90 9 droplets droplet NNS cord-349042-u9svz7pf 90 10 and and CC cord-349042-u9svz7pf 90 11 barcodes barcode VBZ cord-349042-u9svz7pf 90 12 the the DT cord-349042-u9svz7pf 90 13 cDNA cdna NN cord-349042-u9svz7pf 90 14 in in IN cord-349042-u9svz7pf 90 15 each each DT cord-349042-u9svz7pf 90 16 droplet droplet NN cord-349042-u9svz7pf 90 17 during during IN cord-349042-u9svz7pf 90 18 reverse reverse JJ cord-349042-u9svz7pf 90 19 transcription transcription NN cord-349042-u9svz7pf 90 20 . . . cord-349042-u9svz7pf 91 1 Droplets droplet NNS cord-349042-u9svz7pf 91 2 are be VBP cord-349042-u9svz7pf 91 3 then then RB cord-349042-u9svz7pf 91 4 pooled pool VBN cord-349042-u9svz7pf 91 5 , , , cord-349042-u9svz7pf 91 6 and and CC cord-349042-u9svz7pf 91 7 the the DT cord-349042-u9svz7pf 91 8 RNA RNA NNP cord-349042-u9svz7pf 91 9 populations population NNS cord-349042-u9svz7pf 91 10 from from IN cord-349042-u9svz7pf 91 11 all all DT cord-349042-u9svz7pf 91 12 cells cell NNS cord-349042-u9svz7pf 91 13 are be VBP cord-349042-u9svz7pf 91 14 linearly linearly RB cord-349042-u9svz7pf 91 15 amplified amplify VBN cord-349042-u9svz7pf 91 16 and and CC cord-349042-u9svz7pf 91 17 sequenced sequence VBN cord-349042-u9svz7pf 91 18 . . . cord-349042-u9svz7pf 92 1 This this DT cord-349042-u9svz7pf 92 2 application application NN cord-349042-u9svz7pf 92 3 of of IN cord-349042-u9svz7pf 92 4 linear linear JJ cord-349042-u9svz7pf 92 5 aRNA aRNA HYPH cord-349042-u9svz7pf 92 6 amplification amplification NN cord-349042-u9svz7pf 92 7 was be VBD cord-349042-u9svz7pf 92 8 shown show VBN cord-349042-u9svz7pf 92 9 to to TO cord-349042-u9svz7pf 92 10 exhibit exhibit VB cord-349042-u9svz7pf 92 11 low low JJ cord-349042-u9svz7pf 92 12 noise noise NN cord-349042-u9svz7pf 92 13 , , , cord-349042-u9svz7pf 92 14 be be VB cord-349042-u9svz7pf 92 15 low low JJ cord-349042-u9svz7pf 92 16 - - HYPH cord-349042-u9svz7pf 92 17 cost cost NN cord-349042-u9svz7pf 92 18 , , , cord-349042-u9svz7pf 92 19 and and CC cord-349042-u9svz7pf 92 20 be be VB cord-349042-u9svz7pf 92 21 effective effective JJ cord-349042-u9svz7pf 92 22 in in IN cord-349042-u9svz7pf 92 23 isolating isolate VBG cord-349042-u9svz7pf 92 24 and and CC cord-349042-u9svz7pf 92 25 processing process VBG cord-349042-u9svz7pf 92 26 large large JJ cord-349042-u9svz7pf 92 27 numbers number NNS cord-349042-u9svz7pf 92 28 of of IN cord-349042-u9svz7pf 92 29 individual individual JJ cord-349042-u9svz7pf 92 30 cells cell NNS cord-349042-u9svz7pf 92 31 simultaneously simultaneously RB cord-349042-u9svz7pf 92 32 . . . cord-349042-u9svz7pf 93 1 Linear linear JJ cord-349042-u9svz7pf 93 2 aRNA arna NN cord-349042-u9svz7pf 93 3 amplification amplification NN cord-349042-u9svz7pf 93 4 has have VBZ cord-349042-u9svz7pf 93 5 been be VBN cord-349042-u9svz7pf 93 6 used use VBN cord-349042-u9svz7pf 93 7 to to TO cord-349042-u9svz7pf 93 8 study study VB cord-349042-u9svz7pf 93 9 many many JJ cord-349042-u9svz7pf 93 10 human human JJ cord-349042-u9svz7pf 93 11 diseases disease NNS cord-349042-u9svz7pf 93 12 . . . cord-349042-u9svz7pf 94 1 Its -PRON- PRP$ cord-349042-u9svz7pf 94 2 most most RBS cord-349042-u9svz7pf 94 3 prominent prominent JJ cord-349042-u9svz7pf 94 4 application application NN cord-349042-u9svz7pf 94 5 has have VBZ cord-349042-u9svz7pf 94 6 been be VBN cord-349042-u9svz7pf 94 7 to to TO cord-349042-u9svz7pf 94 8 identify identify VB cord-349042-u9svz7pf 94 9 biomarkers biomarker NNS cord-349042-u9svz7pf 94 10 , , , cord-349042-u9svz7pf 94 11 but but CC cord-349042-u9svz7pf 94 12 it -PRON- PRP cord-349042-u9svz7pf 94 13 is be VBZ cord-349042-u9svz7pf 94 14 also also RB cord-349042-u9svz7pf 94 15 used use VBN cord-349042-u9svz7pf 94 16 to to TO cord-349042-u9svz7pf 94 17 identify identify VB cord-349042-u9svz7pf 94 18 potential potential JJ cord-349042-u9svz7pf 94 19 therapeutic therapeutic JJ cord-349042-u9svz7pf 94 20 targets target NNS cord-349042-u9svz7pf 94 21 . . . cord-349042-u9svz7pf 95 1 In in IN cord-349042-u9svz7pf 95 2 many many JJ cord-349042-u9svz7pf 95 3 of of IN cord-349042-u9svz7pf 95 4 the the DT cord-349042-u9svz7pf 95 5 disease disease NN cord-349042-u9svz7pf 95 6 - - HYPH cord-349042-u9svz7pf 95 7 associated associate VBN cord-349042-u9svz7pf 95 8 studies study NNS cord-349042-u9svz7pf 95 9 , , , cord-349042-u9svz7pf 95 10 commercially commercially RB cord-349042-u9svz7pf 95 11 available available JJ cord-349042-u9svz7pf 95 12 versions version NNS cord-349042-u9svz7pf 95 13 of of IN cord-349042-u9svz7pf 95 14 the the DT cord-349042-u9svz7pf 95 15 linear linear JJ cord-349042-u9svz7pf 95 16 amplification amplification NN cord-349042-u9svz7pf 95 17 technology technology NN cord-349042-u9svz7pf 95 18 have have VBP cord-349042-u9svz7pf 95 19 been be VBN cord-349042-u9svz7pf 95 20 used use VBN cord-349042-u9svz7pf 95 21 under under IN cord-349042-u9svz7pf 95 22 trademarked trademark VBN cord-349042-u9svz7pf 95 23 names name NNS cord-349042-u9svz7pf 95 24 , , , cord-349042-u9svz7pf 95 25 including include VBG cord-349042-u9svz7pf 95 26 RiboAmp riboamp JJ cord-349042-u9svz7pf 95 27 ( ( -LRB- cord-349042-u9svz7pf 95 28 Thermo Thermo NNP cord-349042-u9svz7pf 95 29 ) ) -RRB- cord-349042-u9svz7pf 95 30 , , , cord-349042-u9svz7pf 95 31 AmpliScribe AmpliScribe NNP cord-349042-u9svz7pf 95 32 ( ( -LRB- cord-349042-u9svz7pf 95 33 Epicentre Epicentre NNP cord-349042-u9svz7pf 95 34 ) ) -RRB- cord-349042-u9svz7pf 95 35 , , , cord-349042-u9svz7pf 95 36 and and CC cord-349042-u9svz7pf 95 37 MEGAscript MEGAscript NNP cord-349042-u9svz7pf 95 38 ( ( -LRB- cord-349042-u9svz7pf 95 39 Invitrogen Invitrogen NNP cord-349042-u9svz7pf 95 40 ) ) -RRB- cord-349042-u9svz7pf 95 41 . . . cord-349042-u9svz7pf 96 1 Because because IN cord-349042-u9svz7pf 96 2 aRNA aRNA NNP cord-349042-u9svz7pf 96 3 is be VBZ cord-349042-u9svz7pf 96 4 cell cell NN cord-349042-u9svz7pf 96 5 - - HYPH cord-349042-u9svz7pf 96 6 type type NN cord-349042-u9svz7pf 96 7 agnostic agnostic NN cord-349042-u9svz7pf 96 8 , , , cord-349042-u9svz7pf 96 9 it -PRON- PRP cord-349042-u9svz7pf 96 10 can can MD cord-349042-u9svz7pf 96 11 be be VB cord-349042-u9svz7pf 96 12 used use VBN cord-349042-u9svz7pf 96 13 in in IN cord-349042-u9svz7pf 96 14 a a DT cord-349042-u9svz7pf 96 15 variety variety NN cord-349042-u9svz7pf 96 16 of of IN cord-349042-u9svz7pf 96 17 diseaseassociated diseaseassociated JJ cord-349042-u9svz7pf 96 18 cell cell NN cord-349042-u9svz7pf 96 19 types type NNS cord-349042-u9svz7pf 96 20 . . . cord-349042-u9svz7pf 97 1 For for IN cord-349042-u9svz7pf 97 2 example example NN cord-349042-u9svz7pf 97 3 , , , cord-349042-u9svz7pf 97 4 the the DT cord-349042-u9svz7pf 97 5 transcriptome transcriptome NN cord-349042-u9svz7pf 97 6 of of IN cord-349042-u9svz7pf 97 7 platelets platelet NNS cord-349042-u9svz7pf 97 8 isolated isolate VBN cord-349042-u9svz7pf 97 9 from from IN cord-349042-u9svz7pf 97 10 people people NNS cord-349042-u9svz7pf 97 11 with with IN cord-349042-u9svz7pf 97 12 sickle sickle NN cord-349042-u9svz7pf 97 13 cell cell NN cord-349042-u9svz7pf 97 14 disease disease NNP cord-349042-u9svz7pf 97 15 27 27 CD cord-349042-u9svz7pf 97 16 has have VBZ cord-349042-u9svz7pf 97 17 highlighted highlight VBN cord-349042-u9svz7pf 97 18 the the DT cord-349042-u9svz7pf 97 19 role role NN cord-349042-u9svz7pf 97 20 of of IN cord-349042-u9svz7pf 97 21 metabolic metabolic JJ cord-349042-u9svz7pf 97 22 pathways pathway NNS cord-349042-u9svz7pf 97 23 in in IN cord-349042-u9svz7pf 97 24 the the DT cord-349042-u9svz7pf 97 25 disease disease NN cord-349042-u9svz7pf 97 26 . . . cord-349042-u9svz7pf 98 1 Transcriptomic Transcriptomic NNP cord-349042-u9svz7pf 98 2 insights insight NNS cord-349042-u9svz7pf 98 3 into into IN cord-349042-u9svz7pf 98 4 brain brain NN cord-349042-u9svz7pf 98 5 diseases disease NNS cord-349042-u9svz7pf 98 6 such such JJ cord-349042-u9svz7pf 98 7 as as IN cord-349042-u9svz7pf 98 8 schizophrenia schizophrenia NN cord-349042-u9svz7pf 98 9 28 28 CD cord-349042-u9svz7pf 98 10 , , , cord-349042-u9svz7pf 98 11 depression depression NN cord-349042-u9svz7pf 98 12 29 29 CD cord-349042-u9svz7pf 98 13 , , , cord-349042-u9svz7pf 98 14 Parkinson Parkinson NNP cord-349042-u9svz7pf 98 15 's 's POS cord-349042-u9svz7pf 98 16 disease disease NN cord-349042-u9svz7pf 98 17 30 30 CD cord-349042-u9svz7pf 98 18 , , , cord-349042-u9svz7pf 98 19 Alzheimer Alzheimer NNP cord-349042-u9svz7pf 98 20 's 's POS cord-349042-u9svz7pf 98 21 disease disease NN cord-349042-u9svz7pf 98 22 31 31 CD cord-349042-u9svz7pf 98 23 , , , cord-349042-u9svz7pf 98 24 and and CC cord-349042-u9svz7pf 98 25 even even RB cord-349042-u9svz7pf 98 26 brain brain NN cord-349042-u9svz7pf 98 27 microvascular microvascular JJ cord-349042-u9svz7pf 98 28 injury injury NN cord-349042-u9svz7pf 98 29 have have VBP cord-349042-u9svz7pf 98 30 provided provide VBN cord-349042-u9svz7pf 98 31 many many JJ cord-349042-u9svz7pf 98 32 disease disease NN cord-349042-u9svz7pf 98 33 - - HYPH cord-349042-u9svz7pf 98 34 associated associate VBN cord-349042-u9svz7pf 98 35 targets target NNS cord-349042-u9svz7pf 98 36 for for IN cord-349042-u9svz7pf 98 37 further further JJ cord-349042-u9svz7pf 98 38 analysis analysis NN cord-349042-u9svz7pf 98 39 . . . cord-349042-u9svz7pf 99 1 The the DT cord-349042-u9svz7pf 99 2 study study NN cord-349042-u9svz7pf 99 3 of of IN cord-349042-u9svz7pf 99 4 cancer cancer NN cord-349042-u9svz7pf 99 5 has have VBZ cord-349042-u9svz7pf 99 6 benefited benefit VBN cord-349042-u9svz7pf 99 7 preferential preferential JJ cord-349042-u9svz7pf 99 8 amplification amplification NN cord-349042-u9svz7pf 99 9 of of IN cord-349042-u9svz7pf 99 10 certain certain JJ cord-349042-u9svz7pf 99 11 RNA RNA NNP cord-349042-u9svz7pf 99 12 species specie NNS cord-349042-u9svz7pf 99 13 ( ( -LRB- cord-349042-u9svz7pf 99 14 i.e. i.e. FW cord-349042-u9svz7pf 99 15 , , , cord-349042-u9svz7pf 99 16 it -PRON- PRP cord-349042-u9svz7pf 99 17 shows show VBZ cord-349042-u9svz7pf 99 18 no no DT cord-349042-u9svz7pf 99 19 sequence sequence NN cord-349042-u9svz7pf 99 20 preference preference NN cord-349042-u9svz7pf 99 21 ) ) -RRB- cord-349042-u9svz7pf 99 22 nor nor CC cord-349042-u9svz7pf 99 23 creates create VBZ cord-349042-u9svz7pf 99 24 significant significant JJ cord-349042-u9svz7pf 99 25 differences difference NNS cord-349042-u9svz7pf 99 26 in in IN cord-349042-u9svz7pf 99 27 the the DT cord-349042-u9svz7pf 99 28 ratios ratio NNS cord-349042-u9svz7pf 99 29 of of IN cord-349042-u9svz7pf 99 30 specific specific JJ cord-349042-u9svz7pf 99 31 aRNAs arna NNS cord-349042-u9svz7pf 99 32 in in IN cord-349042-u9svz7pf 99 33 the the DT cord-349042-u9svz7pf 99 34 amplified amplify VBN cord-349042-u9svz7pf 99 35 RNA RNA NNP cord-349042-u9svz7pf 99 36 population population NN cord-349042-u9svz7pf 99 37 compared compare VBN cord-349042-u9svz7pf 99 38 with with IN cord-349042-u9svz7pf 99 39 those those DT cord-349042-u9svz7pf 99 40 of of IN cord-349042-u9svz7pf 99 41 the the DT cord-349042-u9svz7pf 99 42 initial initial JJ cord-349042-u9svz7pf 99 43 host host NN cord-349042-u9svz7pf 99 44 cell cell NN cord-349042-u9svz7pf 99 45 mRNA mrna NN cord-349042-u9svz7pf 99 46 abundances abundance NNS cord-349042-u9svz7pf 99 47 3,9 3,9 CD cord-349042-u9svz7pf 99 48 . . . cord-349042-u9svz7pf 100 1 It -PRON- PRP cord-349042-u9svz7pf 100 2 should should MD cord-349042-u9svz7pf 100 3 be be VB cord-349042-u9svz7pf 100 4 noted note VBN cord-349042-u9svz7pf 100 5 that that IN cord-349042-u9svz7pf 100 6 if if IN cord-349042-u9svz7pf 100 7 there there EX cord-349042-u9svz7pf 100 8 were be VBD cord-349042-u9svz7pf 100 9 bias bias NN cord-349042-u9svz7pf 100 10 in in IN cord-349042-u9svz7pf 100 11 amplification amplification NN cord-349042-u9svz7pf 100 12 , , , cord-349042-u9svz7pf 100 13 then then RB cord-349042-u9svz7pf 100 14 the the DT cord-349042-u9svz7pf 100 15 bias bias NN cord-349042-u9svz7pf 100 16 would would MD cord-349042-u9svz7pf 100 17 be be VB cord-349042-u9svz7pf 100 18 linearly linearly RB cord-349042-u9svz7pf 100 19 amplified amplify VBN cord-349042-u9svz7pf 100 20 rather rather RB cord-349042-u9svz7pf 100 21 than than IN cord-349042-u9svz7pf 100 22 exponentially exponentially RB cord-349042-u9svz7pf 100 23 as as IN cord-349042-u9svz7pf 100 24 in in IN cord-349042-u9svz7pf 100 25 PCR PCR NNP cord-349042-u9svz7pf 100 26 amplification amplification NN cord-349042-u9svz7pf 100 27 . . . cord-349042-u9svz7pf 101 1 Furthermore furthermore RB cord-349042-u9svz7pf 101 2 , , , cord-349042-u9svz7pf 101 3 aRNA arna NN cord-349042-u9svz7pf 101 4 has have VBZ cord-349042-u9svz7pf 101 5 been be VBN cord-349042-u9svz7pf 101 6 shown show VBN cord-349042-u9svz7pf 101 7 to to TO cord-349042-u9svz7pf 101 8 provide provide VB cord-349042-u9svz7pf 101 9 an an DT cord-349042-u9svz7pf 101 10 accurate accurate JJ cord-349042-u9svz7pf 101 11 and and CC cord-349042-u9svz7pf 101 12 precise precise JJ cord-349042-u9svz7pf 101 13 amplification amplification NN cord-349042-u9svz7pf 101 14 product product NN cord-349042-u9svz7pf 101 15 [ [ -LRB- cord-349042-u9svz7pf 101 16 9 9 CD cord-349042-u9svz7pf 101 17 ] ] -RRB- cord-349042-u9svz7pf 101 18 [ [ -LRB- cord-349042-u9svz7pf 101 19 10 10 CD cord-349042-u9svz7pf 101 20 ] ] -RRB- cord-349042-u9svz7pf 101 21 [ [ -LRB- cord-349042-u9svz7pf 101 22 11 11 CD cord-349042-u9svz7pf 101 23 ] ] -RRB- cord-349042-u9svz7pf 101 24 [ [ -LRB- cord-349042-u9svz7pf 101 25 12 12 CD cord-349042-u9svz7pf 101 26 ] ] -RRB- cord-349042-u9svz7pf 101 27 . . . cord-349042-u9svz7pf 102 1 aRNA aRNA NNP cord-349042-u9svz7pf 102 2 has have VBZ cord-349042-u9svz7pf 102 3 been be VBN cord-349042-u9svz7pf 102 4 used use VBN cord-349042-u9svz7pf 102 5 extensively extensively RB cord-349042-u9svz7pf 102 6 to to TO cord-349042-u9svz7pf 102 7 generate generate VB cord-349042-u9svz7pf 102 8 probes probe NNS cord-349042-u9svz7pf 102 9 for for IN cord-349042-u9svz7pf 102 10 northern northern JJ cord-349042-u9svz7pf 102 11 and and CC cord-349042-u9svz7pf 102 12 Southern southern JJ cord-349042-u9svz7pf 102 13 blotting blotting NN cord-349042-u9svz7pf 102 14 , , , cord-349042-u9svz7pf 102 15 in in IN cord-349042-u9svz7pf 102 16 tandem tandem NN cord-349042-u9svz7pf 102 17 with with IN cord-349042-u9svz7pf 102 18 PCR PCR NNP cord-349042-u9svz7pf 102 19 13,14 13,14 CD cord-349042-u9svz7pf 102 20 and and CC cord-349042-u9svz7pf 102 21 microarrays microarray NNS cord-349042-u9svz7pf 102 22 4 4 CD cord-349042-u9svz7pf 102 23 , , , cord-349042-u9svz7pf 102 24 12 12 CD cord-349042-u9svz7pf 102 25 , , , cord-349042-u9svz7pf 102 26 15 15 CD cord-349042-u9svz7pf 102 27 , , , cord-349042-u9svz7pf 102 28 16 16 CD cord-349042-u9svz7pf 102 29 , , , cord-349042-u9svz7pf 102 30 and and CC cord-349042-u9svz7pf 102 31 for for IN cord-349042-u9svz7pf 102 32 sequencing sequencing NN cord-349042-u9svz7pf 102 33 of of IN cord-349042-u9svz7pf 102 34 tissue tissue NN cord-349042-u9svz7pf 102 35 - - HYPH cord-349042-u9svz7pf 102 36 isolated isolate VBN cord-349042-u9svz7pf 102 37 RNA RNA NNP cord-349042-u9svz7pf 102 38 7,10,17,18 7,10,17,18 CD cord-349042-u9svz7pf 102 39 . . . cord-349042-u9svz7pf 103 1 The the DT cord-349042-u9svz7pf 103 2 high high JJ cord-349042-u9svz7pf 103 3 yield yield NN cord-349042-u9svz7pf 103 4 , , , cord-349042-u9svz7pf 103 5 accuracy accuracy NN cord-349042-u9svz7pf 103 6 , , , cord-349042-u9svz7pf 103 7 and and CC cord-349042-u9svz7pf 103 8 precision precision NN cord-349042-u9svz7pf 103 9 ( ( -LRB- cord-349042-u9svz7pf 103 10 i.e. i.e. FW cord-349042-u9svz7pf 103 11 , , , cord-349042-u9svz7pf 103 12 similarity similarity NN cord-349042-u9svz7pf 103 13 of of IN cord-349042-u9svz7pf 103 14 same same JJ cord-349042-u9svz7pf 103 15 - - HYPH cord-349042-u9svz7pf 103 16 sample sample NN cord-349042-u9svz7pf 103 17 technical technical JJ cord-349042-u9svz7pf 103 18 repetitions repetition NNS cord-349042-u9svz7pf 103 19 ) ) -RRB- cord-349042-u9svz7pf 103 20 of of IN cord-349042-u9svz7pf 103 21 this this DT cord-349042-u9svz7pf 103 22 transcription transcription NN cord-349042-u9svz7pf 103 23 - - HYPH cord-349042-u9svz7pf 103 24 based base VBN cord-349042-u9svz7pf 103 25 amplification amplification NN cord-349042-u9svz7pf 103 26 system system NN cord-349042-u9svz7pf 103 27 have have VBP cord-349042-u9svz7pf 103 28 led lead VBN cord-349042-u9svz7pf 103 29 to to IN cord-349042-u9svz7pf 103 30 its -PRON- PRP$ cord-349042-u9svz7pf 103 31 use use NN cord-349042-u9svz7pf 103 32 as as IN cord-349042-u9svz7pf 103 33 a a DT cord-349042-u9svz7pf 103 34 reporter reporter NN cord-349042-u9svz7pf 103 35 system system NN cord-349042-u9svz7pf 103 36 to to TO cord-349042-u9svz7pf 103 37 detect detect VB cord-349042-u9svz7pf 103 38 chemical chemical JJ cord-349042-u9svz7pf 103 39 moieties moiety NNS cord-349042-u9svz7pf 103 40 other other JJ cord-349042-u9svz7pf 103 41 than than IN cord-349042-u9svz7pf 103 42 RNA RNA NNP cord-349042-u9svz7pf 103 43 . . . cord-349042-u9svz7pf 104 1 aRNA arna RB cord-349042-u9svz7pf 104 2 - - HYPH cord-349042-u9svz7pf 104 3 associated associate VBN cord-349042-u9svz7pf 104 4 reporter reporter NN cord-349042-u9svz7pf 104 5 systems system NNS cord-349042-u9svz7pf 104 6 for for IN cord-349042-u9svz7pf 104 7 proteins protein NNS cord-349042-u9svz7pf 104 8 , , , cord-349042-u9svz7pf 104 9 RNA rna NN cord-349042-u9svz7pf 104 10 - - HYPH cord-349042-u9svz7pf 104 11 binding bind VBG cord-349042-u9svz7pf 104 12 protein protein NN cord-349042-u9svz7pf 104 13 cargoes cargo NNS cord-349042-u9svz7pf 104 14 , , , cord-349042-u9svz7pf 104 15 and and CC cord-349042-u9svz7pf 104 16 genomic genomic JJ cord-349042-u9svz7pf 104 17 DNA dna NN cord-349042-u9svz7pf 104 18 sequences sequence NNS cord-349042-u9svz7pf 104 19 , , , cord-349042-u9svz7pf 104 20 as as IN cord-349042-u9svz7pf 104 21 illustrated illustrate VBN cord-349042-u9svz7pf 104 22 in in IN cord-349042-u9svz7pf 104 23 Figure Figure NNP cord-349042-u9svz7pf 104 24 2 2 CD cord-349042-u9svz7pf 104 25 , , , cord-349042-u9svz7pf 104 26 benefit benefit NN cord-349042-u9svz7pf 104 27 from from IN cord-349042-u9svz7pf 104 28 the the DT cord-349042-u9svz7pf 104 29 linear linear JJ cord-349042-u9svz7pf 104 30 nature nature NN cord-349042-u9svz7pf 104 31 of of IN cord-349042-u9svz7pf 104 32 the the DT cord-349042-u9svz7pf 104 33 procedure procedure NN cord-349042-u9svz7pf 104 34 and and CC cord-349042-u9svz7pf 104 35 are be VBP cord-349042-u9svz7pf 104 36 further further RB cord-349042-u9svz7pf 104 37 described describe VBN cord-349042-u9svz7pf 104 38 in in IN cord-349042-u9svz7pf 104 39 the the DT cord-349042-u9svz7pf 104 40 following follow VBG cord-349042-u9svz7pf 104 41 sections section NNS cord-349042-u9svz7pf 104 42 . . . cord-349042-u9svz7pf 105 1 Adaptations adaptation NNS cord-349042-u9svz7pf 105 2 to to IN cord-349042-u9svz7pf 105 3 the the DT cord-349042-u9svz7pf 105 4 aRNA arna NN cord-349042-u9svz7pf 105 5 protocol protocol NN cord-349042-u9svz7pf 105 6 that that WDT cord-349042-u9svz7pf 105 7 have have VBP cord-349042-u9svz7pf 105 8 enabled enable VBN cord-349042-u9svz7pf 105 9 advances advance NNS cord-349042-u9svz7pf 105 10 in in IN cord-349042-u9svz7pf 105 11 the the DT cord-349042-u9svz7pf 105 12 use use NN cord-349042-u9svz7pf 105 13 of of IN cord-349042-u9svz7pf 105 14 this this DT cord-349042-u9svz7pf 105 15 technique technique NN cord-349042-u9svz7pf 105 16 to to TO cord-349042-u9svz7pf 105 17 study study VB cord-349042-u9svz7pf 105 18 other other JJ cord-349042-u9svz7pf 105 19 cellular cellular JJ cord-349042-u9svz7pf 105 20 chemicals chemical NNS cord-349042-u9svz7pf 105 21 are be VBP cord-349042-u9svz7pf 105 22 highlighted highlight VBN cord-349042-u9svz7pf 105 23 in in IN cord-349042-u9svz7pf 105 24 Table Table NNP cord-349042-u9svz7pf 105 25 1 1 CD cord-349042-u9svz7pf 105 26 . . . cord-349042-u9svz7pf 106 1 The the DT cord-349042-u9svz7pf 106 2 first first JJ cord-349042-u9svz7pf 106 3 single single JJ cord-349042-u9svz7pf 106 4 - - HYPH cord-349042-u9svz7pf 106 5 cell cell NN cord-349042-u9svz7pf 106 6 RNA RNA NNP cord-349042-u9svz7pf 106 7 analysis analysis NN cord-349042-u9svz7pf 106 8 used use VBN cord-349042-u9svz7pf 106 9 aRNA arna NN cord-349042-u9svz7pf 107 1 ( ( -LRB- cord-349042-u9svz7pf 107 2 Fig Fig NNP cord-349042-u9svz7pf 107 3 . . . cord-349042-u9svz7pf 107 4 2a 2a CD cord-349042-u9svz7pf 107 5 ) ) -RRB- cord-349042-u9svz7pf 108 1 to to TO cord-349042-u9svz7pf 108 2 identify identify VB cord-349042-u9svz7pf 108 3 ribosomal ribosomal JJ cord-349042-u9svz7pf 108 4 RNA RNA NNP cord-349042-u9svz7pf 108 5 in in IN cord-349042-u9svz7pf 108 6 a a DT cord-349042-u9svz7pf 108 7 single single JJ cord-349042-u9svz7pf 108 8 rat rat NN cord-349042-u9svz7pf 108 9 cerebellar cerebellar JJ cord-349042-u9svz7pf 108 10 Purkinje Purkinje NNP cord-349042-u9svz7pf 108 11 cell cell NN cord-349042-u9svz7pf 108 12 2 2 CD cord-349042-u9svz7pf 108 13 . . . cord-349042-u9svz7pf 109 1 This this DT cord-349042-u9svz7pf 109 2 was be VBD cord-349042-u9svz7pf 109 3 followed follow VBN cord-349042-u9svz7pf 109 4 by by IN cord-349042-u9svz7pf 109 5 the the DT cord-349042-u9svz7pf 109 6 development development NN cord-349042-u9svz7pf 109 7 of of IN cord-349042-u9svz7pf 109 8 the the DT cord-349042-u9svz7pf 109 9 first first JJ cord-349042-u9svz7pf 109 10 single single JJ cord-349042-u9svz7pf 109 11 - - HYPH cord-349042-u9svz7pf 109 12 cell cell NN cord-349042-u9svz7pf 109 13 RNA rna NN cord-349042-u9svz7pf 109 14 - - HYPH cord-349042-u9svz7pf 109 15 sequencing sequencing NN cord-349042-u9svz7pf 109 16 library library NN cord-349042-u9svz7pf 109 17 , , , cord-349042-u9svz7pf 109 18 where where WRB cord-349042-u9svz7pf 109 19 aRNA arna NN cord-349042-u9svz7pf 109 20 was be VBD cord-349042-u9svz7pf 109 21 used use VBN cord-349042-u9svz7pf 109 22 on on IN cord-349042-u9svz7pf 109 23 individual individual JJ cord-349042-u9svz7pf 109 24 rat rat NN cord-349042-u9svz7pf 109 25 hippocampal hippocampal JJ cord-349042-u9svz7pf 109 26 neurons neuron NNS cord-349042-u9svz7pf 109 27 3 3 CD cord-349042-u9svz7pf 109 28 and and CC cord-349042-u9svz7pf 109 29 subsequently subsequently RB cord-349042-u9svz7pf 109 30 for for IN cord-349042-u9svz7pf 109 31 differential differential JJ cord-349042-u9svz7pf 109 32 display display NN cord-349042-u9svz7pf 109 33 and and CC cord-349042-u9svz7pf 109 34 Sanger Sanger NNP cord-349042-u9svz7pf 109 35 sequencing sequencing NN cord-349042-u9svz7pf 109 36 of of IN cord-349042-u9svz7pf 109 37 hundreds hundred NNS cord-349042-u9svz7pf 109 38 of of IN cord-349042-u9svz7pf 109 39 RNAs rna NNS cord-349042-u9svz7pf 109 40 5 5 CD cord-349042-u9svz7pf 109 41 from from IN cord-349042-u9svz7pf 109 42 single single JJ cord-349042-u9svz7pf 109 43 neuronal neuronal JJ cord-349042-u9svz7pf 109 44 dendrites dendrite NNS cord-349042-u9svz7pf 109 45 18 18 CD cord-349042-u9svz7pf 109 46 . . . cord-349042-u9svz7pf 110 1 This this DT cord-349042-u9svz7pf 110 2 showed show VBD cord-349042-u9svz7pf 110 3 that that IN cord-349042-u9svz7pf 110 4 aRNA arna NN cord-349042-u9svz7pf 110 5 amplification amplification NN cord-349042-u9svz7pf 110 6 was be VBD cord-349042-u9svz7pf 110 7 sufficiently sufficiently RB cord-349042-u9svz7pf 110 8 sensitive sensitive JJ cord-349042-u9svz7pf 110 9 to to TO cord-349042-u9svz7pf 110 10 facilitate facilitate VB cord-349042-u9svz7pf 110 11 analysis analysis NN cord-349042-u9svz7pf 110 12 of of IN cord-349042-u9svz7pf 110 13 the the DT cord-349042-u9svz7pf 110 14 RNA RNA NNP cord-349042-u9svz7pf 110 15 content content NN cord-349042-u9svz7pf 110 16 of of IN cord-349042-u9svz7pf 110 17 a a DT cord-349042-u9svz7pf 110 18 well well RB cord-349042-u9svz7pf 110 19 - - HYPH cord-349042-u9svz7pf 110 20 defined define VBN cord-349042-u9svz7pf 110 21 subcellular subcellular JJ cord-349042-u9svz7pf 110 22 region region NN cord-349042-u9svz7pf 110 23 of of IN cord-349042-u9svz7pf 110 24 a a DT cord-349042-u9svz7pf 110 25 neuron neuron NN cord-349042-u9svz7pf 110 26 - - HYPH cord-349042-u9svz7pf 110 27 for for IN cord-349042-u9svz7pf 110 28 example example NN cord-349042-u9svz7pf 110 29 , , , cord-349042-u9svz7pf 110 30 a a DT cord-349042-u9svz7pf 110 31 dendrite dendrite NN cord-349042-u9svz7pf 110 32 - - : cord-349042-u9svz7pf 110 33 independently independently RB cord-349042-u9svz7pf 110 34 from from IN cord-349042-u9svz7pf 110 35 the the DT cord-349042-u9svz7pf 110 36 cell cell NN cord-349042-u9svz7pf 110 37 soma soma NNP cord-349042-u9svz7pf 110 38 19 19 CD cord-349042-u9svz7pf 110 39 , , , cord-349042-u9svz7pf 110 40 20 20 CD cord-349042-u9svz7pf 110 41 , , , cord-349042-u9svz7pf 110 42 which which WDT cord-349042-u9svz7pf 110 43 represents represent VBZ cord-349042-u9svz7pf 110 44 a a DT cord-349042-u9svz7pf 110 45 1,000× 1,000× CD cord-349042-u9svz7pf 110 46 larger large JJR cord-349042-u9svz7pf 110 47 volume volume NN cord-349042-u9svz7pf 110 48 . . . cord-349042-u9svz7pf 111 1 A a DT cord-349042-u9svz7pf 111 2 timeline timeline NN cord-349042-u9svz7pf 111 3 highlighting highlight VBG cord-349042-u9svz7pf 111 4 some some DT cord-349042-u9svz7pf 111 5 of of IN cord-349042-u9svz7pf 111 6 the the DT cord-349042-u9svz7pf 111 7 major major JJ cord-349042-u9svz7pf 111 8 scientific scientific JJ cord-349042-u9svz7pf 111 9 advances advance NNS cord-349042-u9svz7pf 111 10 made make VBN cord-349042-u9svz7pf 111 11 with with IN cord-349042-u9svz7pf 111 12 aRNA aRNA NNP cord-349042-u9svz7pf 111 13 is be VBZ cord-349042-u9svz7pf 111 14 presented present VBN cord-349042-u9svz7pf 111 15 in in IN cord-349042-u9svz7pf 111 16 Figure Figure NNP cord-349042-u9svz7pf 111 17 3 3 CD cord-349042-u9svz7pf 111 18 . . . cord-349042-u9svz7pf 112 1 Microarray Microarray NNP cord-349042-u9svz7pf 112 2 - - HYPH cord-349042-u9svz7pf 112 3 based base VBN cord-349042-u9svz7pf 112 4 gene gene NN cord-349042-u9svz7pf 112 5 profiling profiling NN cord-349042-u9svz7pf 112 6 was be VBD cord-349042-u9svz7pf 112 7 performed perform VBN cord-349042-u9svz7pf 112 8 extensively extensively RB cord-349042-u9svz7pf 112 9 before before IN cord-349042-u9svz7pf 112 10 the the DT cord-349042-u9svz7pf 112 11 advent advent NN cord-349042-u9svz7pf 112 12 of of IN cord-349042-u9svz7pf 112 13 RNA RNA NNP cord-349042-u9svz7pf 112 14 - - HYPH cord-349042-u9svz7pf 112 15 seq seq NNP cord-349042-u9svz7pf 112 16 and and CC cord-349042-u9svz7pf 112 17 is be VBZ cord-349042-u9svz7pf 112 18 still still RB cord-349042-u9svz7pf 112 19 used use VBN cord-349042-u9svz7pf 112 20 as as IN cord-349042-u9svz7pf 112 21 an an DT cord-349042-u9svz7pf 112 22 experimental experimental JJ cord-349042-u9svz7pf 112 23 tool tool NN cord-349042-u9svz7pf 112 24 for for IN cord-349042-u9svz7pf 112 25 the the DT cord-349042-u9svz7pf 112 26 direct direct JJ cord-349042-u9svz7pf 112 27 monitoring monitoring NN cord-349042-u9svz7pf 112 28 of of IN cord-349042-u9svz7pf 112 29 large large JJ cord-349042-u9svz7pf 112 30 numbers number NNS cord-349042-u9svz7pf 112 31 of of IN cord-349042-u9svz7pf 112 32 mRNAs mRNAs NNPS cord-349042-u9svz7pf 112 33 in in IN cord-349042-u9svz7pf 112 34 parallel parallel NN cord-349042-u9svz7pf 112 35 with with IN cord-349042-u9svz7pf 112 36 RNA rna NN cord-349042-u9svz7pf 112 37 sequencing sequencing NN cord-349042-u9svz7pf 112 38 . . . cord-349042-u9svz7pf 113 1 The the DT cord-349042-u9svz7pf 113 2 first first JJ cord-349042-u9svz7pf 113 3 transcriptomic transcriptomic JJ cord-349042-u9svz7pf 113 4 - - HYPH cord-349042-u9svz7pf 113 5 level level NN cord-349042-u9svz7pf 113 6 evaluation evaluation NN cord-349042-u9svz7pf 113 7 of of IN cord-349042-u9svz7pf 113 8 linear linear JJ cord-349042-u9svz7pf 113 9 amplified amplify VBN cord-349042-u9svz7pf 113 10 aRNA arna NN cord-349042-u9svz7pf 113 11 for for IN cord-349042-u9svz7pf 113 12 gene gene NN cord-349042-u9svz7pf 113 13 expression expression NN cord-349042-u9svz7pf 113 14 analysis analysis NN cord-349042-u9svz7pf 113 15 by by IN cord-349042-u9svz7pf 113 16 microarray microarray NN cord-349042-u9svz7pf 113 17 was be VBD cord-349042-u9svz7pf 113 18 done do VBN cord-349042-u9svz7pf 113 19 by by IN cord-349042-u9svz7pf 113 20 Lockhart Lockhart NNP cord-349042-u9svz7pf 113 21 et et NNP cord-349042-u9svz7pf 113 22 al al NNP cord-349042-u9svz7pf 113 23 . . . cord-349042-u9svz7pf 114 1 12 12 CD cord-349042-u9svz7pf 114 2 , , , cord-349042-u9svz7pf 114 3 and and CC cord-349042-u9svz7pf 114 4 later later RBR cord-349042-u9svz7pf 114 5 Wang Wang NNP cord-349042-u9svz7pf 114 6 et et NNP cord-349042-u9svz7pf 114 7 al al NNP cord-349042-u9svz7pf 114 8 . . . cord-349042-u9svz7pf 115 1 21 21 CD cord-349042-u9svz7pf 115 2 , , , cord-349042-u9svz7pf 115 3 22 22 CD cord-349042-u9svz7pf 115 4 conducted conduct VBD cord-349042-u9svz7pf 115 5 a a DT cord-349042-u9svz7pf 115 6 statistical statistical JJ cord-349042-u9svz7pf 115 7 assessment assessment NN cord-349042-u9svz7pf 115 8 of of IN cord-349042-u9svz7pf 115 9 aRNA arna NN cord-349042-u9svz7pf 115 10 performance performance NN cord-349042-u9svz7pf 115 11 in in IN cord-349042-u9svz7pf 115 12 microarray microarray NN cord-349042-u9svz7pf 115 13 analysis analysis NN cord-349042-u9svz7pf 115 14 . . . cord-349042-u9svz7pf 116 1 Xu Xu NNP cord-349042-u9svz7pf 116 2 et et NNP cord-349042-u9svz7pf 116 3 al al NNP cord-349042-u9svz7pf 116 4 . . . cord-349042-u9svz7pf 117 1 23 23 CD cord-349042-u9svz7pf 117 2 developed develop VBD cord-349042-u9svz7pf 117 3 a a DT cord-349042-u9svz7pf 117 4 human human JJ cord-349042-u9svz7pf 117 5 transcriptome transcriptome NN cord-349042-u9svz7pf 117 6 microarray microarray NN cord-349042-u9svz7pf 117 7 for for IN cord-349042-u9svz7pf 117 8 high high JJ cord-349042-u9svz7pf 117 9 - - HYPH cord-349042-u9svz7pf 117 10 throughput throughput NN cord-349042-u9svz7pf 117 11 and and CC cord-349042-u9svz7pf 117 12 low low JJ cord-349042-u9svz7pf 117 13 - - HYPH cord-349042-u9svz7pf 117 14 cost cost NN cord-349042-u9svz7pf 117 15 expression expression NN cord-349042-u9svz7pf 117 16 - - HYPH cord-349042-u9svz7pf 117 17 profiling profile VBG cord-349042-u9svz7pf 117 18 analyses analysis NNS cord-349042-u9svz7pf 117 19 in in IN cord-349042-u9svz7pf 117 20 clinical clinical JJ cord-349042-u9svz7pf 117 21 studies study NNS cord-349042-u9svz7pf 117 22 . . . cord-349042-u9svz7pf 118 1 With with IN cord-349042-u9svz7pf 118 2 a a DT cord-349042-u9svz7pf 118 3 small small JJ cord-349042-u9svz7pf 118 4 amount amount NN cord-349042-u9svz7pf 118 5 of of IN cord-349042-u9svz7pf 118 6 human human JJ cord-349042-u9svz7pf 118 7 tissue tissue NN cord-349042-u9svz7pf 118 8 as as IN cord-349042-u9svz7pf 118 9 starting start VBG cord-349042-u9svz7pf 118 10 material material NN cord-349042-u9svz7pf 118 11 , , , cord-349042-u9svz7pf 118 12 they -PRON- PRP cord-349042-u9svz7pf 118 13 linearly linearly RB cord-349042-u9svz7pf 118 14 amplified amplify VBD cord-349042-u9svz7pf 118 15 nanogram nanogram NN cord-349042-u9svz7pf 118 16 amounts amount NNS cord-349042-u9svz7pf 118 17 of of IN cord-349042-u9svz7pf 118 18 total total JJ cord-349042-u9svz7pf 118 19 RNA RNA NNP cord-349042-u9svz7pf 118 20 , , , cord-349042-u9svz7pf 118 21 applied apply VBD cord-349042-u9svz7pf 118 22 the the DT cord-349042-u9svz7pf 118 23 aRNA arna NN cord-349042-u9svz7pf 118 24 to to IN cord-349042-u9svz7pf 118 25 custom custom RB cord-349042-u9svz7pf 118 26 - - HYPH cord-349042-u9svz7pf 118 27 designed design VBN cord-349042-u9svz7pf 118 28 6.9-millionfeature 6.9-millionfeature CD cord-349042-u9svz7pf 118 29 oligonucleotide oligonucleotide JJ cord-349042-u9svz7pf 118 30 arrays array NNS cord-349042-u9svz7pf 118 31 , , , cord-349042-u9svz7pf 118 32 and and CC cord-349042-u9svz7pf 118 33 obtained obtain VBN cord-349042-u9svz7pf 118 34 reproducible reproducible JJ cord-349042-u9svz7pf 118 35 and and CC cord-349042-u9svz7pf 118 36 informative informative JJ cord-349042-u9svz7pf 118 37 analyses analysis NNS cord-349042-u9svz7pf 118 38 of of IN cord-349042-u9svz7pf 118 39 gene gene NN cord-349042-u9svz7pf 118 40 and and CC cord-349042-u9svz7pf 118 41 exon exon NN cord-349042-u9svz7pf 118 42 expression expression NN cord-349042-u9svz7pf 118 43 . . . cord-349042-u9svz7pf 119 1 The the DT cord-349042-u9svz7pf 119 2 use use NN cord-349042-u9svz7pf 119 3 of of IN cord-349042-u9svz7pf 119 4 aRNA arna NN cord-349042-u9svz7pf 119 5 in in IN cord-349042-u9svz7pf 119 6 single single JJ cord-349042-u9svz7pf 119 7 - - HYPH cord-349042-u9svz7pf 119 8 cell cell NN cord-349042-u9svz7pf 119 9 analysis analysis NN cord-349042-u9svz7pf 119 10 increased increase VBD cord-349042-u9svz7pf 119 11 dramatically dramatically RB cord-349042-u9svz7pf 119 12 after after IN cord-349042-u9svz7pf 119 13 RNA RNA NNP cord-349042-u9svz7pf 119 14 - - HYPH cord-349042-u9svz7pf 119 15 seq seq NNP cord-349042-u9svz7pf 119 16 was be VBD cord-349042-u9svz7pf 119 17 introduced introduce VBN cord-349042-u9svz7pf 119 18 . . . cord-349042-u9svz7pf 120 1 There there EX cord-349042-u9svz7pf 120 2 have have VBP cord-349042-u9svz7pf 120 3 been be VBN cord-349042-u9svz7pf 120 4 many many JJ cord-349042-u9svz7pf 120 5 singlecell singlecell NNP cord-349042-u9svz7pf 120 6 aRNA aRNA NNP cord-349042-u9svz7pf 120 7 studies study NNS cord-349042-u9svz7pf 120 8 , , , cord-349042-u9svz7pf 120 9 but but CC cord-349042-u9svz7pf 121 1 recently recently RB cord-349042-u9svz7pf 121 2 Spaethling Spaethling NNP cord-349042-u9svz7pf 121 3 et et FW cord-349042-u9svz7pf 121 4 al al NNP cord-349042-u9svz7pf 121 5 . . . cord-349042-u9svz7pf 122 1 8 8 CD cord-349042-u9svz7pf 122 2 identified identify VBN cord-349042-u9svz7pf 122 3 > > NN cord-349042-u9svz7pf 122 4 500 500 CD cord-349042-u9svz7pf 122 5 G g NN cord-349042-u9svz7pf 122 6 - - HYPH cord-349042-u9svz7pf 122 7 protein protein NN cord-349042-u9svz7pf 122 8 - - HYPH cord-349042-u9svz7pf 122 9 coupled couple VBN cord-349042-u9svz7pf 122 10 receptor receptor NN cord-349042-u9svz7pf 122 11 mRNAs mrnas CD cord-349042-u9svz7pf 122 12 , , , cord-349042-u9svz7pf 122 13 including include VBG cord-349042-u9svz7pf 122 14 mRNAs mRNAs NNPS cord-349042-u9svz7pf 122 15 for for IN cord-349042-u9svz7pf 122 16 dozens dozen NNS cord-349042-u9svz7pf 122 17 of of IN cord-349042-u9svz7pf 122 18 orphan orphan NN cord-349042-u9svz7pf 122 19 G g NN cord-349042-u9svz7pf 122 20 - - HYPH cord-349042-u9svz7pf 122 21 protein protein NN cord-349042-u9svz7pf 122 22 - - HYPH cord-349042-u9svz7pf 122 23 coupled couple VBN cord-349042-u9svz7pf 122 24 receptors receptor NNS cord-349042-u9svz7pf 122 25 in in IN cord-349042-u9svz7pf 122 26 serotonergic serotonergic JJ cord-349042-u9svz7pf 122 27 neurons neuron NNS cord-349042-u9svz7pf 122 28 , , , cord-349042-u9svz7pf 122 29 after after IN cord-349042-u9svz7pf 122 30 three three CD cord-349042-u9svz7pf 122 31 rounds round NNS cord-349042-u9svz7pf 122 32 of of IN cord-349042-u9svz7pf 122 33 aRNA arna NN cord-349042-u9svz7pf 122 34 amplification amplification NN cord-349042-u9svz7pf 122 35 and and CC cord-349042-u9svz7pf 122 36 sequencing sequencing NN cord-349042-u9svz7pf 122 37 of of IN cord-349042-u9svz7pf 122 38 RNA RNA NNP cord-349042-u9svz7pf 122 39 libraries library NNS cord-349042-u9svz7pf 122 40 . . . cord-349042-u9svz7pf 123 1 They -PRON- PRP cord-349042-u9svz7pf 123 2 also also RB cord-349042-u9svz7pf 123 3 validated validate VBD cord-349042-u9svz7pf 123 4 the the DT cord-349042-u9svz7pf 123 5 presence presence NN cord-349042-u9svz7pf 123 6 of of IN cord-349042-u9svz7pf 123 7 the the DT cord-349042-u9svz7pf 123 8 receptors receptor NNS cord-349042-u9svz7pf 123 9 by by IN cord-349042-u9svz7pf 123 10 2 2 CD cord-349042-u9svz7pf 123 11 . . . cord-349042-u9svz7pf 124 1 One one CD cord-349042-u9svz7pf 124 2 or or CC cord-349042-u9svz7pf 124 3 two two CD cord-349042-u9svz7pf 124 4 rounds round NNS cord-349042-u9svz7pf 124 5 of of IN cord-349042-u9svz7pf 124 6 aRNA arna NN cord-349042-u9svz7pf 124 7 labeled label VBN cord-349042-u9svz7pf 124 8 with with IN cord-349042-u9svz7pf 124 9 dye dye NN cord-349042-u9svz7pf 124 10 , , , cord-349042-u9svz7pf 124 11 incorporating incorporate VBG cord-349042-u9svz7pf 124 12 a a DT cord-349042-u9svz7pf 124 13 cleavage cleavage NN cord-349042-u9svz7pf 124 14 step step NN cord-349042-u9svz7pf 124 15 during during IN cord-349042-u9svz7pf 124 16 in in IN cord-349042-u9svz7pf 124 17 vitro vitro FW cord-349042-u9svz7pf 124 18 RNA RNA NNP cord-349042-u9svz7pf 124 19 transcription transcription NN cord-349042-u9svz7pf 124 20 . . . cord-349042-u9svz7pf 125 1 3 3 LS cord-349042-u9svz7pf 125 2 . . . cord-349042-u9svz7pf 126 1 aRNA arna LS cord-349042-u9svz7pf 126 2 modified modify VBN cord-349042-u9svz7pf 126 3 for for IN cord-349042-u9svz7pf 126 4 template template NN cord-349042-u9svz7pf 126 5 switching switch VBG cord-349042-u9svz7pf 126 6 for for IN cord-349042-u9svz7pf 126 7 better well JJR cord-349042-u9svz7pf 126 8 5′-end 5′-end CD cord-349042-u9svz7pf 126 9 representation representation NN cord-349042-u9svz7pf 126 10 in in IN cord-349042-u9svz7pf 126 11 the the DT cord-349042-u9svz7pf 126 12 aRNA arna NN cord-349042-u9svz7pf 126 13 products product NNS cord-349042-u9svz7pf 126 14 . . . cord-349042-u9svz7pf 127 1 4 4 LS cord-349042-u9svz7pf 127 2 . . . cord-349042-u9svz7pf 128 1 Two two CD cord-349042-u9svz7pf 128 2 rounds round NNS cord-349042-u9svz7pf 128 3 of of IN cord-349042-u9svz7pf 128 4 aRNA arna NN cord-349042-u9svz7pf 128 5 adapted adapt VBN cord-349042-u9svz7pf 128 6 to to TO cord-349042-u9svz7pf 128 7 amplify amplify VB cord-349042-u9svz7pf 128 8 double double RB cord-349042-u9svz7pf 128 9 - - HYPH cord-349042-u9svz7pf 128 10 stranded stranded JJ cord-349042-u9svz7pf 128 11 CHIP chip NN cord-349042-u9svz7pf 128 12 - - HYPH cord-349042-u9svz7pf 128 13 chip chip NN cord-349042-u9svz7pf 128 14 DNA dna NN cord-349042-u9svz7pf 128 15 fragments fragment NNS cord-349042-u9svz7pf 128 16 . . . cord-349042-u9svz7pf 129 1 After after IN cord-349042-u9svz7pf 129 2 the the DT cord-349042-u9svz7pf 129 3 addition addition NN cord-349042-u9svz7pf 129 4 of of IN cord-349042-u9svz7pf 129 5 poly(dT poly(dT NNP cord-349042-u9svz7pf 129 6 ) ) -RRB- cord-349042-u9svz7pf 129 7 to to IN cord-349042-u9svz7pf 129 8 the the DT cord-349042-u9svz7pf 129 9 DNA dna NN cord-349042-u9svz7pf 129 10 template template NN cord-349042-u9svz7pf 129 11 , , , cord-349042-u9svz7pf 129 12 oligo(dA)-T7 oligo(da)-t7 CD cord-349042-u9svz7pf 129 13 is be VBZ cord-349042-u9svz7pf 129 14 annealed anneal VBN cord-349042-u9svz7pf 129 15 . . . cord-349042-u9svz7pf 130 1 The the DT cord-349042-u9svz7pf 130 2 resulting result VBG cord-349042-u9svz7pf 130 3 double double RB cord-349042-u9svz7pf 130 4 - - HYPH cord-349042-u9svz7pf 130 5 stranded strand VBN cord-349042-u9svz7pf 130 6 DNA dna NN cord-349042-u9svz7pf 130 7 fragment fragment NN cord-349042-u9svz7pf 130 8 contains contain VBZ cord-349042-u9svz7pf 130 9 a a DT cord-349042-u9svz7pf 130 10 functional functional JJ cord-349042-u9svz7pf 130 11 T7 t7 NN cord-349042-u9svz7pf 130 12 promoter promoter NN cord-349042-u9svz7pf 130 13 that that WDT cord-349042-u9svz7pf 130 14 drives drive VBZ cord-349042-u9svz7pf 130 15 the the DT cord-349042-u9svz7pf 130 16 in in FW cord-349042-u9svz7pf 130 17 vitro vitro FW cord-349042-u9svz7pf 130 18 transcription transcription NN cord-349042-u9svz7pf 130 19 reaction reaction NN cord-349042-u9svz7pf 130 20 , , , cord-349042-u9svz7pf 130 21 generating generate VBG cord-349042-u9svz7pf 130 22 multiple multiple JJ cord-349042-u9svz7pf 130 23 RNA RNA NNP cord-349042-u9svz7pf 130 24 copies copy NNS cord-349042-u9svz7pf 130 25 of of IN cord-349042-u9svz7pf 130 26 the the DT cord-349042-u9svz7pf 130 27 DNA dna NN cord-349042-u9svz7pf 130 28 template template NN cord-349042-u9svz7pf 130 29 . . . cord-349042-u9svz7pf 131 1 5 5 LS cord-349042-u9svz7pf 131 2 . . . cord-349042-u9svz7pf 132 1 aRNA aRNA NNP cord-349042-u9svz7pf 133 1 modified modify VBN cord-349042-u9svz7pf 133 2 for for IN cord-349042-u9svz7pf 133 3 long long JJ cord-349042-u9svz7pf 133 4 SAGE SAGE NNP cord-349042-u9svz7pf 133 5 ( ( -LRB- cord-349042-u9svz7pf 133 6 serial serial JJ cord-349042-u9svz7pf 133 7 analysis analysis NN cord-349042-u9svz7pf 133 8 of of IN cord-349042-u9svz7pf 133 9 gene gene NN cord-349042-u9svz7pf 133 10 expression expression NN cord-349042-u9svz7pf 133 11 ) ) -RRB- cord-349042-u9svz7pf 133 12 for for IN cord-349042-u9svz7pf 133 13 detection detection NN cord-349042-u9svz7pf 133 14 of of IN cord-349042-u9svz7pf 133 15 large large JJ cord-349042-u9svz7pf 133 16 - - HYPH cord-349042-u9svz7pf 133 17 scale scale NN cord-349042-u9svz7pf 133 18 gene gene NN cord-349042-u9svz7pf 133 19 expression expression NN cord-349042-u9svz7pf 133 20 from from IN cord-349042-u9svz7pf 133 21 microdissected microdissected JJ cord-349042-u9svz7pf 133 22 primary primary JJ cord-349042-u9svz7pf 133 23 tissue tissue NN cord-349042-u9svz7pf 133 24 . . . cord-349042-u9svz7pf 134 1 After after IN cord-349042-u9svz7pf 134 2 one one CD cord-349042-u9svz7pf 134 3 round round NN cord-349042-u9svz7pf 134 4 of of IN cord-349042-u9svz7pf 134 5 aRNA arna NN cord-349042-u9svz7pf 134 6 , , , cord-349042-u9svz7pf 134 7 first first JJ cord-349042-u9svz7pf 134 8 - - HYPH cord-349042-u9svz7pf 134 9 strand strand NN cord-349042-u9svz7pf 134 10 cDNA cdna NN cord-349042-u9svz7pf 134 11 is be VBZ cord-349042-u9svz7pf 134 12 primed prime VBN cord-349042-u9svz7pf 134 13 with with IN cord-349042-u9svz7pf 134 14 SAGE SAGE NNP cord-349042-u9svz7pf 134 15 random random JJ cord-349042-u9svz7pf 134 16 primer primer NN cord-349042-u9svz7pf 134 17 . . . cord-349042-u9svz7pf 135 1 Second second JJ cord-349042-u9svz7pf 135 2 - - HYPH cord-349042-u9svz7pf 135 3 strand strand NN cord-349042-u9svz7pf 135 4 cDNA cdna NN cord-349042-u9svz7pf 135 5 is be VBZ cord-349042-u9svz7pf 135 6 then then RB cord-349042-u9svz7pf 135 7 primed prime VBN cord-349042-u9svz7pf 135 8 with with IN cord-349042-u9svz7pf 135 9 Dynabeads Dynabeads NNP cord-349042-u9svz7pf 135 10 oligo(dT oligo(dt CD cord-349042-u9svz7pf 135 11 ) ) -RRB- cord-349042-u9svz7pf 135 12 primer primer NN cord-349042-u9svz7pf 135 13 and and CC cord-349042-u9svz7pf 135 14 used use VBN cord-349042-u9svz7pf 135 15 for for IN cord-349042-u9svz7pf 135 16 standard standard JJ cord-349042-u9svz7pf 135 17 SAGE SAGE NNP cord-349042-u9svz7pf 135 18 library library NN cord-349042-u9svz7pf 135 19 generation generation NN cord-349042-u9svz7pf 135 20 . . . cord-349042-u9svz7pf 136 1 6 6 LS cord-349042-u9svz7pf 136 2 . . . cord-349042-u9svz7pf 137 1 aRNA aRNA NNP cord-349042-u9svz7pf 138 1 modified modify VBN cord-349042-u9svz7pf 138 2 for for IN cord-349042-u9svz7pf 138 3 amplification amplification NN cord-349042-u9svz7pf 138 4 on on IN cord-349042-u9svz7pf 138 5 immobilized immobilized JJ cord-349042-u9svz7pf 138 6 beads bead NNS cord-349042-u9svz7pf 138 7 . . . cord-349042-u9svz7pf 139 1 After after IN cord-349042-u9svz7pf 139 2 coupling coupling NN cord-349042-u9svz7pf 139 3 of of IN cord-349042-u9svz7pf 139 4 the the DT cord-349042-u9svz7pf 139 5 oligo(dT)-T7 oligo(dt)-t7 JJ cord-349042-u9svz7pf 139 6 primer primer NN cord-349042-u9svz7pf 139 7 to to IN cord-349042-u9svz7pf 139 8 magnetic magnetic JJ cord-349042-u9svz7pf 139 9 beads bead NNS cord-349042-u9svz7pf 139 10 , , , cord-349042-u9svz7pf 139 11 aRNA arna NN cord-349042-u9svz7pf 139 12 is be VBZ cord-349042-u9svz7pf 139 13 synthesized synthesize VBN cord-349042-u9svz7pf 139 14 directly directly RB cord-349042-u9svz7pf 139 15 on on IN cord-349042-u9svz7pf 139 16 the the DT cord-349042-u9svz7pf 139 17 beads bead NNS cord-349042-u9svz7pf 139 18 to to TO cord-349042-u9svz7pf 139 19 increase increase VB cord-349042-u9svz7pf 139 20 the the DT cord-349042-u9svz7pf 139 21 yield yield NN cord-349042-u9svz7pf 139 22 . . . cord-349042-u9svz7pf 140 1 7 7 CD cord-349042-u9svz7pf 140 2 . . . cord-349042-u9svz7pf 141 1 Modification modification NN cord-349042-u9svz7pf 141 2 of of IN cord-349042-u9svz7pf 141 3 aRNA arna NN cord-349042-u9svz7pf 142 1 so so IN cord-349042-u9svz7pf 142 2 that that IN cord-349042-u9svz7pf 142 3 it -PRON- PRP cord-349042-u9svz7pf 142 4 produces produce VBZ cord-349042-u9svz7pf 142 5 sense sense NN cord-349042-u9svz7pf 142 6 RNA RNA NNP cord-349042-u9svz7pf 142 7 . . . cord-349042-u9svz7pf 143 1 The the DT cord-349042-u9svz7pf 143 2 use use NN cord-349042-u9svz7pf 143 3 of of IN cord-349042-u9svz7pf 143 4 random random JJ cord-349042-u9svz7pf 143 5 primers primer NNS cord-349042-u9svz7pf 143 6 containing contain VBG cord-349042-u9svz7pf 143 7 an an DT cord-349042-u9svz7pf 143 8 upstream upstream JJ cord-349042-u9svz7pf 143 9 T3 T3 NNP cord-349042-u9svz7pf 143 10 promoter promoter NN cord-349042-u9svz7pf 143 11 sequence sequence NN cord-349042-u9svz7pf 143 12 to to IN cord-349042-u9svz7pf 143 13 prime prime JJ cord-349042-u9svz7pf 143 14 second second JJ cord-349042-u9svz7pf 143 15 - - HYPH cord-349042-u9svz7pf 143 16 strand strand NN cord-349042-u9svz7pf 143 17 cDNA cdna NN cord-349042-u9svz7pf 143 18 synthesis synthesis NN cord-349042-u9svz7pf 143 19 enables enable VBZ cord-349042-u9svz7pf 143 20 in in IN cord-349042-u9svz7pf 143 21 vitro vitro FW cord-349042-u9svz7pf 143 22 transcription transcription NN cord-349042-u9svz7pf 143 23 from from IN cord-349042-u9svz7pf 143 24 T3 T3 NNP cord-349042-u9svz7pf 143 25 promoter promoter NN cord-349042-u9svz7pf 143 26 sequences sequence NNS cord-349042-u9svz7pf 143 27 incorporated incorporate VBD cord-349042-u9svz7pf 143 28 into into IN cord-349042-u9svz7pf 143 29 the the DT cord-349042-u9svz7pf 143 30 single single JJ cord-349042-u9svz7pf 143 31 - - HYPH cord-349042-u9svz7pf 143 32 strand strand NN cord-349042-u9svz7pf 143 33 cDNA cdna NN cord-349042-u9svz7pf 143 34 , , , cord-349042-u9svz7pf 143 35 thus thus RB cord-349042-u9svz7pf 143 36 producing produce VBG cord-349042-u9svz7pf 143 37 amplified amplify VBN cord-349042-u9svz7pf 143 38 RNA RNA NNP cord-349042-u9svz7pf 143 39 in in IN cord-349042-u9svz7pf 143 40 the the DT cord-349042-u9svz7pf 143 41 sense sense NN cord-349042-u9svz7pf 143 42 orientation orientation NN cord-349042-u9svz7pf 143 43 by by IN cord-349042-u9svz7pf 143 44 T3 T3 NNP cord-349042-u9svz7pf 143 45 polymerase polymerase NN cord-349042-u9svz7pf 143 46 . . . cord-349042-u9svz7pf 144 1 high high JJ cord-349042-u9svz7pf 144 2 - - HYPH cord-349042-u9svz7pf 144 3 resolution resolution NN cord-349042-u9svz7pf 144 4 detection detection NN cord-349042-u9svz7pf 144 5 of of IN cord-349042-u9svz7pf 144 6 biological biological JJ cord-349042-u9svz7pf 144 7 processes process NNS cord-349042-u9svz7pf 144 8 such such JJ cord-349042-u9svz7pf 144 9 as as IN cord-349042-u9svz7pf 144 10 stochastic stochastic JJ cord-349042-u9svz7pf 144 11 copying copying NN cord-349042-u9svz7pf 144 12 of of IN cord-349042-u9svz7pf 144 13 DNA dna NN cord-349042-u9svz7pf 144 14 replication replication NN cord-349042-u9svz7pf 144 15 origins origin NNS cord-349042-u9svz7pf 144 16 . . . cord-349042-u9svz7pf 145 1 The the DT cord-349042-u9svz7pf 145 2 aRNA arna NN cord-349042-u9svz7pf 145 3 procedure procedure NN cord-349042-u9svz7pf 145 4 has have VBZ cord-349042-u9svz7pf 145 5 been be VBN cord-349042-u9svz7pf 145 6 adapted adapt VBN cord-349042-u9svz7pf 145 7 to to TO cord-349042-u9svz7pf 145 8 identify identify VB cord-349042-u9svz7pf 145 9 mRNA mrna NN cord-349042-u9svz7pf 145 10 targets target NNS cord-349042-u9svz7pf 145 11 that that WDT cord-349042-u9svz7pf 145 12 are be VBP cord-349042-u9svz7pf 145 13 associated associate VBN cord-349042-u9svz7pf 145 14 with with IN cord-349042-u9svz7pf 145 15 specific specific JJ cord-349042-u9svz7pf 145 16 RNA rna NN cord-349042-u9svz7pf 145 17 - - HYPH cord-349042-u9svz7pf 145 18 binding bind VBG cord-349042-u9svz7pf 145 19 proteins protein NNS cord-349042-u9svz7pf 145 20 ( ( -LRB- cord-349042-u9svz7pf 145 21 RBPs RBPs NNP cord-349042-u9svz7pf 145 22 ) ) -RRB- cord-349042-u9svz7pf 145 23 ( ( -LRB- cord-349042-u9svz7pf 145 24 Fig Fig NNP cord-349042-u9svz7pf 145 25 . . . cord-349042-u9svz7pf 145 26 2b 2b NNP cord-349042-u9svz7pf 145 27 ) ) -RRB- cord-349042-u9svz7pf 145 28 . . . cord-349042-u9svz7pf 146 1 Miyashiro Miyashiro NNP cord-349042-u9svz7pf 146 2 et et NNP cord-349042-u9svz7pf 146 3 al al NNP cord-349042-u9svz7pf 146 4 . . . cord-349042-u9svz7pf 147 1 42 42 CD cord-349042-u9svz7pf 147 2 , , , cord-349042-u9svz7pf 147 3 43 43 CD cord-349042-u9svz7pf 147 4 developed develop VBD cord-349042-u9svz7pf 147 5 a a DT cord-349042-u9svz7pf 147 6 technique technique NN cord-349042-u9svz7pf 147 7 called call VBN cord-349042-u9svz7pf 147 8 antibody antibody NN cord-349042-u9svz7pf 147 9 - - HYPH cord-349042-u9svz7pf 147 10 positioned position VBN cord-349042-u9svz7pf 147 11 RNA RNA NNP cord-349042-u9svz7pf 147 12 amplification amplification NN cord-349042-u9svz7pf 147 13 ( ( -LRB- cord-349042-u9svz7pf 147 14 APRA APRA NNP cord-349042-u9svz7pf 147 15 ) ) -RRB- cord-349042-u9svz7pf 147 16 , , , cord-349042-u9svz7pf 147 17 in in IN cord-349042-u9svz7pf 147 18 which which WDT cord-349042-u9svz7pf 147 19 an an DT cord-349042-u9svz7pf 147 20 RBP rbp NN cord-349042-u9svz7pf 147 21 - - HYPH cord-349042-u9svz7pf 147 22 specific specific JJ cord-349042-u9svz7pf 147 23 antibody antibody NN cord-349042-u9svz7pf 147 24 is be VBZ cord-349042-u9svz7pf 147 25 conjugated conjugate VBN cord-349042-u9svz7pf 147 26 to to IN cord-349042-u9svz7pf 147 27 a a DT cord-349042-u9svz7pf 147 28 singlestranded singlestranded JJ cord-349042-u9svz7pf 147 29 oligonucleotide oligonucleotide NN cord-349042-u9svz7pf 147 30 containing contain VBG cord-349042-u9svz7pf 147 31 a a DT cord-349042-u9svz7pf 147 32 restriction restriction NN cord-349042-u9svz7pf 147 33 enzyme enzyme NN cord-349042-u9svz7pf 147 34 site site NN cord-349042-u9svz7pf 147 35 , , , cord-349042-u9svz7pf 147 36 a a DT cord-349042-u9svz7pf 147 37 T7 T7 NNP cord-349042-u9svz7pf 147 38 RNA RNA NNP cord-349042-u9svz7pf 147 39 polymerase polymerase NN cord-349042-u9svz7pf 147 40 promoter promoter NN cord-349042-u9svz7pf 147 41 sequence sequence NN cord-349042-u9svz7pf 147 42 at at IN cord-349042-u9svz7pf 147 43 its -PRON- PRP$ cord-349042-u9svz7pf 147 44 5′ 5′ CD cord-349042-u9svz7pf 147 45 end end NN cord-349042-u9svz7pf 147 46 , , , cord-349042-u9svz7pf 147 47 and and CC cord-349042-u9svz7pf 147 48 a a DT cord-349042-u9svz7pf 147 49 15-base 15-base CD cord-349042-u9svz7pf 147 50 degenerate degenerate JJ cord-349042-u9svz7pf 147 51 nucleotide nucleotide JJ cord-349042-u9svz7pf 147 52 sequence sequence NN cord-349042-u9svz7pf 147 53 at at IN cord-349042-u9svz7pf 147 54 its -PRON- PRP$ cord-349042-u9svz7pf 147 55 3′ 3′ CD cord-349042-u9svz7pf 147 56 end end NN cord-349042-u9svz7pf 147 57 . . . cord-349042-u9svz7pf 148 1 When when WRB cord-349042-u9svz7pf 148 2 the the DT cord-349042-u9svz7pf 148 3 antibody antibody NN cord-349042-u9svz7pf 148 4 - - HYPH cord-349042-u9svz7pf 148 5 oligo oligo NN cord-349042-u9svz7pf 148 6 conjugate conjugate NN cord-349042-u9svz7pf 148 7 is be VBZ cord-349042-u9svz7pf 148 8 applied apply VBN cord-349042-u9svz7pf 148 9 to to IN cord-349042-u9svz7pf 148 10 fixed fix VBN cord-349042-u9svz7pf 148 11 cells cell NNS cord-349042-u9svz7pf 148 12 , , , cord-349042-u9svz7pf 148 13 the the DT cord-349042-u9svz7pf 148 14 oligonucleotide oligonucleotide JJ cord-349042-u9svz7pf 148 15 acts act VBZ cord-349042-u9svz7pf 148 16 as as IN cord-349042-u9svz7pf 148 17 an an DT cord-349042-u9svz7pf 148 18 APRA APRA NNP cord-349042-u9svz7pf 148 19 primer primer NN cord-349042-u9svz7pf 148 20 and and CC cord-349042-u9svz7pf 148 21 is be VBZ cord-349042-u9svz7pf 148 22 positioned position VBN cord-349042-u9svz7pf 148 23 near near IN cord-349042-u9svz7pf 148 24 the the DT cord-349042-u9svz7pf 148 25 RNAs rna NNS cord-349042-u9svz7pf 148 26 that that WDT cord-349042-u9svz7pf 148 27 are be VBP cord-349042-u9svz7pf 148 28 associated associate VBN cord-349042-u9svz7pf 148 29 with with IN cord-349042-u9svz7pf 148 30 the the DT cord-349042-u9svz7pf 148 31 RBP RBP NNP cord-349042-u9svz7pf 148 32 , , , cord-349042-u9svz7pf 148 33 and and CC cord-349042-u9svz7pf 148 34 the the DT cord-349042-u9svz7pf 148 35 degenerate degenerate JJ cord-349042-u9svz7pf 148 36 sequence sequence NN cord-349042-u9svz7pf 148 37 of of IN cord-349042-u9svz7pf 148 38 the the DT cord-349042-u9svz7pf 148 39 antibody antibody NN cord-349042-u9svz7pf 148 40 - - HYPH cord-349042-u9svz7pf 148 41 bound bind VBN cord-349042-u9svz7pf 148 42 oligonucleotide oligonucleotide NN cord-349042-u9svz7pf 148 43 can can MD cord-349042-u9svz7pf 148 44 prime prime JJ cord-349042-u9svz7pf 148 45 first first JJ cord-349042-u9svz7pf 148 46 - - HYPH cord-349042-u9svz7pf 148 47 strand strand NN cord-349042-u9svz7pf 148 48 cDNA cdna NN cord-349042-u9svz7pf 148 49 synthesis synthesis NN cord-349042-u9svz7pf 148 50 directly directly RB cord-349042-u9svz7pf 148 51 in in IN cord-349042-u9svz7pf 148 52 situ situ NN cord-349042-u9svz7pf 148 53 in in IN cord-349042-u9svz7pf 148 54 the the DT cord-349042-u9svz7pf 148 55 fixed fix VBN cord-349042-u9svz7pf 148 56 tissue tissue NN cord-349042-u9svz7pf 148 57 section section NN cord-349042-u9svz7pf 148 58 44 44 CD cord-349042-u9svz7pf 148 59 . . . cord-349042-u9svz7pf 149 1 The the DT cord-349042-u9svz7pf 149 2 antibody antibody NN cord-349042-u9svz7pf 149 3 - - HYPH cord-349042-u9svz7pf 149 4 cDNA cdna NN cord-349042-u9svz7pf 149 5 complex complex NN cord-349042-u9svz7pf 149 6 is be VBZ cord-349042-u9svz7pf 149 7 then then RB cord-349042-u9svz7pf 149 8 removed remove VBN cord-349042-u9svz7pf 149 9 from from IN cord-349042-u9svz7pf 149 10 the the DT cord-349042-u9svz7pf 149 11 fixed fix VBN cord-349042-u9svz7pf 149 12 cells cell NNS cord-349042-u9svz7pf 149 13 , , , cord-349042-u9svz7pf 149 14 and and CC cord-349042-u9svz7pf 149 15 second second JJ cord-349042-u9svz7pf 149 16 - - HYPH cord-349042-u9svz7pf 149 17 strand strand NN cord-349042-u9svz7pf 149 18 cDNA cdna NN cord-349042-u9svz7pf 149 19 is be VBZ cord-349042-u9svz7pf 149 20 synthesized synthesize VBN cord-349042-u9svz7pf 149 21 in in IN cord-349042-u9svz7pf 149 22 vitro vitro FW cord-349042-u9svz7pf 149 23 . . . cord-349042-u9svz7pf 150 1 The the DT cord-349042-u9svz7pf 150 2 double double RB cord-349042-u9svz7pf 150 3 - - HYPH cord-349042-u9svz7pf 150 4 stranded strand VBN cord-349042-u9svz7pf 150 5 cDNA cdna NN cord-349042-u9svz7pf 150 6 is be VBZ cord-349042-u9svz7pf 150 7 removed remove VBN cord-349042-u9svz7pf 150 8 from from IN cord-349042-u9svz7pf 150 9 the the DT cord-349042-u9svz7pf 150 10 antibody antibody NN cord-349042-u9svz7pf 150 11 by by IN cord-349042-u9svz7pf 150 12 restriction restriction NN cord-349042-u9svz7pf 150 13 enzyme enzyme NN cord-349042-u9svz7pf 150 14 digestion digestion NN cord-349042-u9svz7pf 150 15 , , , cord-349042-u9svz7pf 150 16 and and CC cord-349042-u9svz7pf 150 17 aRNA arna NN cord-349042-u9svz7pf 150 18 is be VBZ cord-349042-u9svz7pf 150 19 obtained obtain VBN cord-349042-u9svz7pf 150 20 by by RP cord-349042-u9svz7pf 150 21 in in IN cord-349042-u9svz7pf 150 22 vitro vitro FW cord-349042-u9svz7pf 150 23 transcription transcription NN cord-349042-u9svz7pf 150 24 followed follow VBN cord-349042-u9svz7pf 150 25 by by IN cord-349042-u9svz7pf 150 26 two two CD cord-349042-u9svz7pf 150 27 rounds round NNS cord-349042-u9svz7pf 150 28 of of IN cord-349042-u9svz7pf 150 29 aRNA arna NN cord-349042-u9svz7pf 150 30 amplification amplification NN cord-349042-u9svz7pf 150 31 . . . cord-349042-u9svz7pf 151 1 Using use VBG cord-349042-u9svz7pf 151 2 the the DT cord-349042-u9svz7pf 151 3 APRA APRA NNP cord-349042-u9svz7pf 151 4 procedure procedure NN cord-349042-u9svz7pf 151 5 , , , cord-349042-u9svz7pf 151 6 the the DT cord-349042-u9svz7pf 151 7 authors author NNS cord-349042-u9svz7pf 151 8 were be VBD cord-349042-u9svz7pf 151 9 able able JJ cord-349042-u9svz7pf 151 10 to to TO cord-349042-u9svz7pf 151 11 isolate isolate VB cord-349042-u9svz7pf 151 12 RNA RNA NNP cord-349042-u9svz7pf 151 13 cargoes cargo NNS cord-349042-u9svz7pf 151 14 associated associate VBN cord-349042-u9svz7pf 151 15 with with IN cord-349042-u9svz7pf 151 16 fragile fragile JJ cord-349042-u9svz7pf 151 17 X x NN cord-349042-u9svz7pf 151 18 mental mental JJ cord-349042-u9svz7pf 151 19 retardation retardation NN cord-349042-u9svz7pf 151 20 protein protein NN cord-349042-u9svz7pf 151 21 ( ( -LRB- cord-349042-u9svz7pf 151 22 FMRP FMRP NNP cord-349042-u9svz7pf 151 23 ) ) -RRB- cord-349042-u9svz7pf 151 24 and and CC cord-349042-u9svz7pf 151 25 identify identify VB cord-349042-u9svz7pf 151 26 hundreds hundred NNS cord-349042-u9svz7pf 151 27 of of IN cord-349042-u9svz7pf 151 28 previously previously RB cord-349042-u9svz7pf 151 29 unidentified unidentified JJ cord-349042-u9svz7pf 151 30 putative putative JJ cord-349042-u9svz7pf 151 31 FMRP FMRP NNP cord-349042-u9svz7pf 151 32 targets target NNS cord-349042-u9svz7pf 151 33 . . . cord-349042-u9svz7pf 152 1 Among among IN cord-349042-u9svz7pf 152 2 the the DT cord-349042-u9svz7pf 152 3 FMRP FMRP NNP cord-349042-u9svz7pf 152 4 APRA APRA NNP cord-349042-u9svz7pf 152 5 targets target NNS cord-349042-u9svz7pf 152 6 that that WDT cord-349042-u9svz7pf 152 7 were be VBD cord-349042-u9svz7pf 152 8 tested test VBN cord-349042-u9svz7pf 152 9 , , , cord-349042-u9svz7pf 152 10 > > XX cord-349042-u9svz7pf 152 11 60 60 CD cord-349042-u9svz7pf 152 12 % % NN cord-349042-u9svz7pf 152 13 were be VBD cord-349042-u9svz7pf 152 14 confirmed confirm VBN cord-349042-u9svz7pf 152 15 as as IN cord-349042-u9svz7pf 152 16 FMRP FMRP NNP cord-349042-u9svz7pf 152 17 cargoes cargo NNS cord-349042-u9svz7pf 152 18 via via IN cord-349042-u9svz7pf 152 19 filter filter NN cord-349042-u9svz7pf 152 20 binding binding NN cord-349042-u9svz7pf 152 21 or or CC cord-349042-u9svz7pf 152 22 ultraviolet ultraviolet JJ cord-349042-u9svz7pf 152 23 cross cross NN cord-349042-u9svz7pf 152 24 - - JJ cord-349042-u9svz7pf 152 25 linking link VBG cord-349042-u9svz7pf 152 26 assays assay NNS cord-349042-u9svz7pf 152 27 to to TO cord-349042-u9svz7pf 152 28 directly directly RB cord-349042-u9svz7pf 152 29 assess assess VB cord-349042-u9svz7pf 152 30 interaction interaction NN cord-349042-u9svz7pf 152 31 with with IN cord-349042-u9svz7pf 152 32 FMRP FMRP NNP cord-349042-u9svz7pf 152 33 and and CC cord-349042-u9svz7pf 152 34 mRNA mrna NN cord-349042-u9svz7pf 153 1 targets target NNS cord-349042-u9svz7pf 153 2 43 43 CD cord-349042-u9svz7pf 153 3 . . . cord-349042-u9svz7pf 154 1 from from IN cord-349042-u9svz7pf 154 2 aRNA aRNA NNP cord-349042-u9svz7pf 154 3 analysis analysis NN cord-349042-u9svz7pf 154 4 of of IN cord-349042-u9svz7pf 154 5 a a DT cord-349042-u9svz7pf 154 6 variety variety NN cord-349042-u9svz7pf 154 7 of of IN cord-349042-u9svz7pf 154 8 cancers cancer NNS cord-349042-u9svz7pf 154 9 , , , cord-349042-u9svz7pf 154 10 including include VBG cord-349042-u9svz7pf 154 11 breast breast NN cord-349042-u9svz7pf 154 12 cancer cancer NN cord-349042-u9svz7pf 154 13 32 32 CD cord-349042-u9svz7pf 154 14 , , , cord-349042-u9svz7pf 154 15 ductal ductal JJ cord-349042-u9svz7pf 154 16 cancer cancer NN cord-349042-u9svz7pf 154 17 33 33 CD cord-349042-u9svz7pf 154 18 , , , cord-349042-u9svz7pf 154 19 liver liver NN cord-349042-u9svz7pf 154 20 metastases metastasis NNS cord-349042-u9svz7pf 154 21 34 34 CD cord-349042-u9svz7pf 154 22 , , , cord-349042-u9svz7pf 154 23 prostate prostate NN cord-349042-u9svz7pf 154 24 cancer cancer NN cord-349042-u9svz7pf 154 25 35 35 CD cord-349042-u9svz7pf 154 26 , , , cord-349042-u9svz7pf 154 27 and and CC cord-349042-u9svz7pf 154 28 various various JJ cord-349042-u9svz7pf 154 29 brain brain NN cord-349042-u9svz7pf 154 30 cancers cancer NNS cord-349042-u9svz7pf 154 31 36 36 CD cord-349042-u9svz7pf 154 32 , , , cord-349042-u9svz7pf 154 33 37 37 CD cord-349042-u9svz7pf 154 34 . . . cord-349042-u9svz7pf 155 1 An an DT cord-349042-u9svz7pf 155 2 interesting interesting JJ cord-349042-u9svz7pf 155 3 application application NN cord-349042-u9svz7pf 155 4 of of IN cord-349042-u9svz7pf 155 5 linear linear JJ cord-349042-u9svz7pf 155 6 aRNA arna NN cord-349042-u9svz7pf 155 7 amplification amplification NN cord-349042-u9svz7pf 155 8 to to IN cord-349042-u9svz7pf 155 9 the the DT cord-349042-u9svz7pf 155 10 study study NN cord-349042-u9svz7pf 155 11 of of IN cord-349042-u9svz7pf 155 12 pancreatic pancreatic JJ cord-349042-u9svz7pf 155 13 cancer cancer NN cord-349042-u9svz7pf 155 14 involved involve VBD cord-349042-u9svz7pf 155 15 the the DT cord-349042-u9svz7pf 155 16 identification identification NN cord-349042-u9svz7pf 155 17 of of IN cord-349042-u9svz7pf 155 18 pancreatic pancreatic JJ cord-349042-u9svz7pf 155 19 tumor tumor NN cord-349042-u9svz7pf 155 20 markers marker NNS cord-349042-u9svz7pf 155 21 in in IN cord-349042-u9svz7pf 155 22 saliva saliva NN cord-349042-u9svz7pf 155 23 , , , cord-349042-u9svz7pf 155 24 suggesting suggest VBG cord-349042-u9svz7pf 155 25 that that IN cord-349042-u9svz7pf 155 26 tumor tumor NN cord-349042-u9svz7pf 155 27 - - HYPH cord-349042-u9svz7pf 155 28 derived derive VBN cord-349042-u9svz7pf 155 29 exosomes exosome NNS cord-349042-u9svz7pf 155 30 can can MD cord-349042-u9svz7pf 155 31 provide provide VB cord-349042-u9svz7pf 155 32 salivary salivary JJ cord-349042-u9svz7pf 155 33 biomarkers biomarker NNS cord-349042-u9svz7pf 155 34 of of IN cord-349042-u9svz7pf 155 35 disease disease NN cord-349042-u9svz7pf 155 36 38 38 CD cord-349042-u9svz7pf 155 37 . . . cord-349042-u9svz7pf 156 1 In in IN cord-349042-u9svz7pf 156 2 addition addition NN cord-349042-u9svz7pf 156 3 , , , cord-349042-u9svz7pf 156 4 infection infection NN cord-349042-u9svz7pf 156 5 by by IN cord-349042-u9svz7pf 156 6 pathogenic pathogenic JJ cord-349042-u9svz7pf 156 7 microorganisms microorganism NNS cord-349042-u9svz7pf 156 8 has have VBZ cord-349042-u9svz7pf 156 9 been be VBN cord-349042-u9svz7pf 156 10 identified identify VBN cord-349042-u9svz7pf 156 11 in in IN cord-349042-u9svz7pf 156 12 human human JJ cord-349042-u9svz7pf 156 13 samples sample NNS cord-349042-u9svz7pf 156 14 by by IN cord-349042-u9svz7pf 156 15 linear linear JJ cord-349042-u9svz7pf 156 16 aRNA arna NN cord-349042-u9svz7pf 156 17 amplification amplification NN cord-349042-u9svz7pf 156 18 , , , cord-349042-u9svz7pf 156 19 either either CC cord-349042-u9svz7pf 156 20 directly directly RB cord-349042-u9svz7pf 156 21 through through IN cord-349042-u9svz7pf 156 22 detection detection NN cord-349042-u9svz7pf 156 23 of of IN cord-349042-u9svz7pf 156 24 microorganismderived microorganismderived JJ cord-349042-u9svz7pf 156 25 RNAs rna NNS cord-349042-u9svz7pf 156 26 , , , cord-349042-u9svz7pf 156 27 or or CC cord-349042-u9svz7pf 156 28 through through IN cord-349042-u9svz7pf 156 29 biomarker biomarker NN cord-349042-u9svz7pf 156 30 surrogates surrogate NNS cord-349042-u9svz7pf 156 31 in in IN cord-349042-u9svz7pf 156 32 cases case NNS cord-349042-u9svz7pf 156 33 where where WRB cord-349042-u9svz7pf 156 34 changes change NNS cord-349042-u9svz7pf 156 35 in in IN cord-349042-u9svz7pf 156 36 RNA RNA NNP cord-349042-u9svz7pf 156 37 abundance abundance NN cord-349042-u9svz7pf 156 38 for for IN cord-349042-u9svz7pf 156 39 host host NN cord-349042-u9svz7pf 156 40 genes gene NNS cord-349042-u9svz7pf 156 41 known know VBN cord-349042-u9svz7pf 156 42 to to TO cord-349042-u9svz7pf 156 43 be be VB cord-349042-u9svz7pf 156 44 modulated modulate VBN cord-349042-u9svz7pf 156 45 by by IN cord-349042-u9svz7pf 156 46 infection infection NN cord-349042-u9svz7pf 156 47 are be VBP cord-349042-u9svz7pf 156 48 detected detect VBN cord-349042-u9svz7pf 156 49 . . . cord-349042-u9svz7pf 157 1 This this DT cord-349042-u9svz7pf 157 2 is be VBZ cord-349042-u9svz7pf 157 3 exemplified exemplify VBN cord-349042-u9svz7pf 157 4 by by IN cord-349042-u9svz7pf 157 5 the the DT cord-349042-u9svz7pf 157 6 detection detection NN cord-349042-u9svz7pf 157 7 of of IN cord-349042-u9svz7pf 157 8 HIV HIV NNP cord-349042-u9svz7pf 157 9 39 39 CD cord-349042-u9svz7pf 157 10 , , , cord-349042-u9svz7pf 157 11 influenza influenza NN cord-349042-u9svz7pf 157 12 40 40 CD cord-349042-u9svz7pf 157 13 , , , cord-349042-u9svz7pf 157 14 and and CC cord-349042-u9svz7pf 157 15 severe severe JJ cord-349042-u9svz7pf 157 16 acute acute JJ cord-349042-u9svz7pf 157 17 respiratory respiratory JJ cord-349042-u9svz7pf 157 18 syndrome syndrome NN cord-349042-u9svz7pf 157 19 ( ( -LRB- cord-349042-u9svz7pf 157 20 SARS SARS NNP cord-349042-u9svz7pf 157 21 ) ) -RRB- cord-349042-u9svz7pf 158 1 coronavirus coronavirus NNP cord-349042-u9svz7pf 159 1 41 41 CD cord-349042-u9svz7pf 159 2 infections infection NNS cord-349042-u9svz7pf 159 3 . . . cord-349042-u9svz7pf 160 1 Most most RBS cord-349042-u9svz7pf 160 2 recently recently RB cord-349042-u9svz7pf 160 3 , , , cord-349042-u9svz7pf 160 4 linear linear JJ cord-349042-u9svz7pf 160 5 aRNA arna NN cord-349042-u9svz7pf 160 6 amplification amplification NN cord-349042-u9svz7pf 160 7 was be VBD cord-349042-u9svz7pf 160 8 used use VBN cord-349042-u9svz7pf 160 9 by by IN cord-349042-u9svz7pf 160 10 Chen Chen NNP cord-349042-u9svz7pf 160 11 et et NNP cord-349042-u9svz7pf 160 12 al al NNP cord-349042-u9svz7pf 160 13 . . . cord-349042-u9svz7pf 161 1 11 11 CD cord-349042-u9svz7pf 161 2 for for IN cord-349042-u9svz7pf 161 3 whole whole JJ cord-349042-u9svz7pf 161 4 - - HYPH cord-349042-u9svz7pf 161 5 genome genome NN cord-349042-u9svz7pf 161 6 sequencing sequencing NN cord-349042-u9svz7pf 161 7 in in IN cord-349042-u9svz7pf 161 8 a a DT cord-349042-u9svz7pf 161 9 method method NN cord-349042-u9svz7pf 161 10 called call VBN cord-349042-u9svz7pf 161 11 linear linear JJ cord-349042-u9svz7pf 161 12 amplification amplification NN cord-349042-u9svz7pf 161 13 via via IN cord-349042-u9svz7pf 161 14 transposon transposon NN cord-349042-u9svz7pf 161 15 insertion insertion NN cord-349042-u9svz7pf 161 16 ( ( -LRB- cord-349042-u9svz7pf 161 17 LIANTI LIANTI NNP cord-349042-u9svz7pf 161 18 ) ) -RRB- cord-349042-u9svz7pf 162 1 ( ( -LRB- cord-349042-u9svz7pf 162 2 Fig Fig NNP cord-349042-u9svz7pf 162 3 . . . cord-349042-u9svz7pf 162 4 2d 2d NNP cord-349042-u9svz7pf 162 5 ) ) -RRB- cord-349042-u9svz7pf 162 6 . . . cord-349042-u9svz7pf 163 1 This this DT cord-349042-u9svz7pf 163 2 procedure procedure NN cord-349042-u9svz7pf 163 3 enables enable VBZ cord-349042-u9svz7pf 163 4 single single JJ cord-349042-u9svz7pf 163 5 - - HYPH cord-349042-u9svz7pf 163 6 cell cell NN cord-349042-u9svz7pf 163 7 whole whole JJ cord-349042-u9svz7pf 163 8 - - HYPH cord-349042-u9svz7pf 163 9 genome genome NN cord-349042-u9svz7pf 163 10 amplification amplification NN cord-349042-u9svz7pf 163 11 to to TO cord-349042-u9svz7pf 163 12 facilitate facilitate VB cord-349042-u9svz7pf 163 13 sequencing sequencing NN cord-349042-u9svz7pf 163 14 of of IN cord-349042-u9svz7pf 163 15 95 95 CD cord-349042-u9svz7pf 163 16 % % NN cord-349042-u9svz7pf 163 17 of of IN cord-349042-u9svz7pf 163 18 the the DT cord-349042-u9svz7pf 163 19 single single JJ cord-349042-u9svz7pf 163 20 - - HYPH cord-349042-u9svz7pf 163 21 cell cell NN cord-349042-u9svz7pf 163 22 genomic genomic JJ cord-349042-u9svz7pf 163 23 DNA dna NN cord-349042-u9svz7pf 163 24 , , , cord-349042-u9svz7pf 163 25 as as RB cord-349042-u9svz7pf 163 26 well well RB cord-349042-u9svz7pf 163 27 as as IN cord-349042-u9svz7pf 163 28 detection detection NN cord-349042-u9svz7pf 163 29 of of IN cord-349042-u9svz7pf 163 30 single single JJ cord-349042-u9svz7pf 163 31 - - HYPH cord-349042-u9svz7pf 163 32 nucleotide nucleotide JJ cord-349042-u9svz7pf 163 33 variation variation NN cord-349042-u9svz7pf 163 34 with with IN cord-349042-u9svz7pf 163 35 kilobase kilobase NNP cord-349042-u9svz7pf 163 36 resolution resolution NN cord-349042-u9svz7pf 163 37 . . . cord-349042-u9svz7pf 164 1 In in IN cord-349042-u9svz7pf 164 2 LIANTI LIANTI NNP cord-349042-u9svz7pf 164 3 , , , cord-349042-u9svz7pf 164 4 the the DT cord-349042-u9svz7pf 164 5 T7 T7 NNP cord-349042-u9svz7pf 164 6 RNA RNA NNP cord-349042-u9svz7pf 164 7 polymerase polymerase NN cord-349042-u9svz7pf 164 8 promoter promoter NN cord-349042-u9svz7pf 164 9 sequence sequence NN cord-349042-u9svz7pf 164 10 is be VBZ cord-349042-u9svz7pf 164 11 incorporated incorporate VBN cord-349042-u9svz7pf 164 12 into into IN cord-349042-u9svz7pf 164 13 DNA dna NN cord-349042-u9svz7pf 164 14 associated associate VBN cord-349042-u9svz7pf 164 15 with with IN cord-349042-u9svz7pf 164 16 the the DT cord-349042-u9svz7pf 164 17 T5 T5 NNP cord-349042-u9svz7pf 164 18 transposome transposome NN cord-349042-u9svz7pf 164 19 , , , cord-349042-u9svz7pf 164 20 which which WDT cord-349042-u9svz7pf 164 21 , , , cord-349042-u9svz7pf 164 22 after after IN cord-349042-u9svz7pf 164 23 insertion insertion NN cord-349042-u9svz7pf 164 24 into into IN cord-349042-u9svz7pf 164 25 the the DT cord-349042-u9svz7pf 164 26 genomic genomic JJ cord-349042-u9svz7pf 164 27 DNA dna NN cord-349042-u9svz7pf 164 28 , , , cord-349042-u9svz7pf 164 29 embeds embed VBZ cord-349042-u9svz7pf 164 30 the the DT cord-349042-u9svz7pf 164 31 T7 T7 NNP cord-349042-u9svz7pf 164 32 promoter promoter NN cord-349042-u9svz7pf 164 33 into into IN cord-349042-u9svz7pf 164 34 the the DT cord-349042-u9svz7pf 164 35 transposon transposon NN cord-349042-u9svz7pf 164 36 - - HYPH cord-349042-u9svz7pf 164 37 flanked flank VBN cord-349042-u9svz7pf 164 38 DNA dna NN cord-349042-u9svz7pf 164 39 . . . cord-349042-u9svz7pf 165 1 After after IN cord-349042-u9svz7pf 165 2 the the DT cord-349042-u9svz7pf 165 3 addition addition NN cord-349042-u9svz7pf 165 4 of of IN cord-349042-u9svz7pf 165 5 T7 T7 NNP cord-349042-u9svz7pf 165 6 RNA RNA NNP cord-349042-u9svz7pf 165 7 polymerase polymerase NN cord-349042-u9svz7pf 165 8 , , , cord-349042-u9svz7pf 165 9 multiple multiple JJ cord-349042-u9svz7pf 165 10 RNA RNA NNP cord-349042-u9svz7pf 165 11 copies copy NNS cord-349042-u9svz7pf 165 12 of of IN cord-349042-u9svz7pf 165 13 the the DT cord-349042-u9svz7pf 165 14 genomic genomic JJ cord-349042-u9svz7pf 165 15 DNA dna NN cord-349042-u9svz7pf 165 16 are be VBP cord-349042-u9svz7pf 165 17 synthesized synthesize VBN cord-349042-u9svz7pf 165 18 . . . cord-349042-u9svz7pf 166 1 The the DT cord-349042-u9svz7pf 166 2 LIANTI LIANTI NNP cord-349042-u9svz7pf 166 3 - - HYPH cord-349042-u9svz7pf 166 4 produced produce VBN cord-349042-u9svz7pf 166 5 aRNA aRNA , cord-349042-u9svz7pf 166 6 is be VBZ cord-349042-u9svz7pf 166 7 reverse reverse RB cord-349042-u9svz7pf 166 8 - - HYPH cord-349042-u9svz7pf 166 9 transcribed transcribe VBN cord-349042-u9svz7pf 166 10 and and CC cord-349042-u9svz7pf 166 11 made make VBN cord-349042-u9svz7pf 166 12 into into IN cord-349042-u9svz7pf 166 13 a a DT cord-349042-u9svz7pf 166 14 sequencing sequencing NN cord-349042-u9svz7pf 166 15 library library NN cord-349042-u9svz7pf 166 16 for for IN cord-349042-u9svz7pf 166 17 subsequent subsequent JJ cord-349042-u9svz7pf 166 18 analysis analysis NN cord-349042-u9svz7pf 166 19 . . . cord-349042-u9svz7pf 167 1 The the DT cord-349042-u9svz7pf 167 2 high high JJ cord-349042-u9svz7pf 167 3 fidelity fidelity NN cord-349042-u9svz7pf 167 4 and and CC cord-349042-u9svz7pf 167 5 yield yield NN cord-349042-u9svz7pf 167 6 of of IN cord-349042-u9svz7pf 167 7 aRNA arna NN cord-349042-u9svz7pf 167 8 permits permit VBZ cord-349042-u9svz7pf 167 9 Linear Linear NNP cord-349042-u9svz7pf 167 10 RNA RNA NNP cord-349042-u9svz7pf 167 11 coupled couple VBN cord-349042-u9svz7pf 167 12 with with IN cord-349042-u9svz7pf 167 13 microarray microarray NN cord-349042-u9svz7pf 167 14 analysis analysis NN cord-349042-u9svz7pf 167 15 ( ( -LRB- cord-349042-u9svz7pf 167 16 12 12 CD cord-349042-u9svz7pf 167 17 ) ) -RRB- cord-349042-u9svz7pf 168 1 Linear Linear NNP cord-349042-u9svz7pf 168 2 RNA RNA NNP cord-349042-u9svz7pf 168 3 used use VBD cord-349042-u9svz7pf 168 4 for for IN cord-349042-u9svz7pf 168 5 protein protein NN cord-349042-u9svz7pf 168 6 detection detection NN cord-349042-u9svz7pf 168 7 ( ( -LRB- cord-349042-u9svz7pf 168 8 IDAT IDAT NNP cord-349042-u9svz7pf 168 9 ; ; : cord-349042-u9svz7pf 168 10 45 45 CD cord-349042-u9svz7pf 168 11 ) ) -RRB- cord-349042-u9svz7pf 169 1 Linear Linear NNP cord-349042-u9svz7pf 169 2 RNA RNA NNP cord-349042-u9svz7pf 169 3 used use VBD cord-349042-u9svz7pf 169 4 for for IN cord-349042-u9svz7pf 169 5 genome genome NN cord-349042-u9svz7pf 169 6 sequencing sequencing NN cord-349042-u9svz7pf 170 1 ( ( -LRB- cord-349042-u9svz7pf 170 2 11 11 CD cord-349042-u9svz7pf 170 3 ) ) -RRB- cord-349042-u9svz7pf 170 4 Detection Detection NNP cord-349042-u9svz7pf 170 5 of of IN cord-349042-u9svz7pf 170 6 RNA RNA NNP cord-349042-u9svz7pf 170 7 - - HYPH cord-349042-u9svz7pf 170 8 binding bind VBG cord-349042-u9svz7pf 170 9 protein protein NN cord-349042-u9svz7pf 170 10 cargoes cargo NNS cord-349042-u9svz7pf 170 11 ( ( -LRB- cord-349042-u9svz7pf 170 12 43 43 CD cord-349042-u9svz7pf 170 13 ) ) -RRB- cord-349042-u9svz7pf 170 14 Detection detection NN cord-349042-u9svz7pf 170 15 of of IN cord-349042-u9svz7pf 170 16 ChIP chip NN cord-349042-u9svz7pf 170 17 sequences sequence NNS cord-349042-u9svz7pf 170 18 ( ( -LRB- cord-349042-u9svz7pf 170 19 47 47 CD cord-349042-u9svz7pf 170 20 ) ) -RRB- cord-349042-u9svz7pf 171 1 Linear Linear NNP cord-349042-u9svz7pf 171 2 RNA RNA NNP cord-349042-u9svz7pf 171 3 coupled couple VBN cord-349042-u9svz7pf 171 4 with with IN cord-349042-u9svz7pf 171 5 PCR PCR NNP cord-349042-u9svz7pf 171 6 ( ( -LRB- cord-349042-u9svz7pf 171 7 13 13 CD cord-349042-u9svz7pf 171 8 ) ) -RRB- cord-349042-u9svz7pf 171 9 High high JJ cord-349042-u9svz7pf 171 10 - - HYPH cord-349042-u9svz7pf 171 11 throughput throughput NN cord-349042-u9svz7pf 171 12 aRNA aRNA HYPH cord-349042-u9svz7pf 171 13 CEL cel NN cord-349042-u9svz7pf 171 14 - - HYPH cord-349042-u9svz7pf 171 15 seq seq NNP cord-349042-u9svz7pf 172 1 ( ( -LRB- cord-349042-u9svz7pf 172 2 9 9 LS cord-349042-u9svz7pf 172 3 ) ) -RRB- cord-349042-u9svz7pf 172 4 High high JJ cord-349042-u9svz7pf 172 5 - - HYPH cord-349042-u9svz7pf 172 6 throughput throughput NN cord-349042-u9svz7pf 172 7 aRNA arna NN cord-349042-u9svz7pf 172 8 InDrop indrop NN cord-349042-u9svz7pf 172 9 ( ( -LRB- cord-349042-u9svz7pf 172 10 26 26 CD cord-349042-u9svz7pf 172 11 ) ) -RRB- cord-349042-u9svz7pf 172 12 High high JJ cord-349042-u9svz7pf 172 13 - - HYPH cord-349042-u9svz7pf 172 14 throughput throughput NN cord-349042-u9svz7pf 172 15 aRNA aRNA HYPH cord-349042-u9svz7pf 172 16 MARS MARS NNP cord-349042-u9svz7pf 172 17 - - HYPH cord-349042-u9svz7pf 172 18 seq seq NNP cord-349042-u9svz7pf 172 19 ( ( -LRB- cord-349042-u9svz7pf 172 20 17 17 CD cord-349042-u9svz7pf 172 21 ) ) -RRB- cord-349042-u9svz7pf 172 22 High high JJ cord-349042-u9svz7pf 172 23 - - HYPH cord-349042-u9svz7pf 172 24 throughput throughput NN cord-349042-u9svz7pf 172 25 aRNA aRNA HYPH cord-349042-u9svz7pf 172 26 microwell microwell NNP cord-349042-u9svz7pf 172 27 ( ( -LRB- cord-349042-u9svz7pf 172 28 51 51 CD cord-349042-u9svz7pf 172 29 ) ) -RRB- cord-349042-u9svz7pf 173 1 Linear Linear NNP cord-349042-u9svz7pf 173 2 RNA RNA NNP cord-349042-u9svz7pf 173 3 used use VBD cord-349042-u9svz7pf 173 4 for for IN cord-349042-u9svz7pf 173 5 fixed fix VBN cord-349042-u9svz7pf 173 6 - - HYPH cord-349042-u9svz7pf 173 7 tissue tissue NN cord-349042-u9svz7pf 173 8 transcriptomics transcriptomic NNS cord-349042-u9svz7pf 173 9 ( ( -LRB- cord-349042-u9svz7pf 173 10 14 14 CD cord-349042-u9svz7pf 173 11 ) ) -RRB- cord-349042-u9svz7pf 174 1 aRNA arna RB cord-349042-u9svz7pf 174 2 on on IN cord-349042-u9svz7pf 174 3 immobilized immobilized JJ cord-349042-u9svz7pf 174 4 beads bead NNS cord-349042-u9svz7pf 174 5 ( ( -LRB- cord-349042-u9svz7pf 174 6 49 49 CD cord-349042-u9svz7pf 174 7 ) ) -RRB- cord-349042-u9svz7pf 174 8 Sensitive sensitive JJ cord-349042-u9svz7pf 174 9 fluorescent fluorescent NN cord-349042-u9svz7pf 174 10 aRNA aRNA , cord-349042-u9svz7pf 174 11 for for IN cord-349042-u9svz7pf 174 12 protein protein NN cord-349042-u9svz7pf 174 13 detection detection NN cord-349042-u9svz7pf 174 14 ( ( -LRB- cord-349042-u9svz7pf 174 15 FACTT FACTT NNP cord-349042-u9svz7pf 174 16 ; ; : cord-349042-u9svz7pf 174 17 46 46 CD cord-349042-u9svz7pf 174 18 ) ) -RRB- cord-349042-u9svz7pf 174 19 usually usually RB cord-349042-u9svz7pf 174 20 involves involve VBZ cord-349042-u9svz7pf 174 21 independent independent JJ cord-349042-u9svz7pf 174 22 isolation isolation NN cord-349042-u9svz7pf 174 23 of of IN cord-349042-u9svz7pf 174 24 the the DT cord-349042-u9svz7pf 174 25 additional additional JJ cord-349042-u9svz7pf 174 26 chemical chemical NN cord-349042-u9svz7pf 174 27 to to TO cord-349042-u9svz7pf 174 28 be be VB cord-349042-u9svz7pf 174 29 quantified quantify VBN cord-349042-u9svz7pf 174 30 , , , cord-349042-u9svz7pf 174 31 which which WDT cord-349042-u9svz7pf 174 32 risks risk VBZ cord-349042-u9svz7pf 174 33 differential differential JJ cord-349042-u9svz7pf 174 34 loss loss NN cord-349042-u9svz7pf 174 35 of of IN cord-349042-u9svz7pf 174 36 material material NN cord-349042-u9svz7pf 174 37 and and CC cord-349042-u9svz7pf 174 38 inaccurate inaccurate JJ cord-349042-u9svz7pf 174 39 interpretation interpretation NN cord-349042-u9svz7pf 174 40 of of IN cord-349042-u9svz7pf 174 41 data datum NNS cord-349042-u9svz7pf 174 42 . . . cord-349042-u9svz7pf 175 1 The the DT cord-349042-u9svz7pf 175 2 linear linear JJ cord-349042-u9svz7pf 175 3 aRNA arna NN cord-349042-u9svz7pf 175 4 reporting report VBG cord-349042-u9svz7pf 175 5 system system NN cord-349042-u9svz7pf 175 6 , , , cord-349042-u9svz7pf 175 7 however however RB cord-349042-u9svz7pf 175 8 , , , cord-349042-u9svz7pf 175 9 provides provide VBZ cord-349042-u9svz7pf 175 10 an an DT cord-349042-u9svz7pf 175 11 opportunity opportunity NN cord-349042-u9svz7pf 175 12 to to TO cord-349042-u9svz7pf 175 13 detect detect VB cord-349042-u9svz7pf 175 14 multiple multiple JJ cord-349042-u9svz7pf 175 15 biological biological JJ cord-349042-u9svz7pf 175 16 entities entity NNS cord-349042-u9svz7pf 175 17 in in IN cord-349042-u9svz7pf 175 18 a a DT cord-349042-u9svz7pf 175 19 single single JJ cord-349042-u9svz7pf 175 20 sample sample NN cord-349042-u9svz7pf 175 21 without without IN cord-349042-u9svz7pf 175 22 specific specific JJ cord-349042-u9svz7pf 175 23 isolation isolation NN cord-349042-u9svz7pf 175 24 . . . cord-349042-u9svz7pf 176 1 This this DT cord-349042-u9svz7pf 176 2 eliminates eliminate VBZ cord-349042-u9svz7pf 176 3 sample sample NN cord-349042-u9svz7pf 176 4 loss loss NN cord-349042-u9svz7pf 176 5 while while IN cord-349042-u9svz7pf 176 6 harmonizing harmonize VBG cord-349042-u9svz7pf 176 7 the the DT cord-349042-u9svz7pf 176 8 detection detection NN cord-349042-u9svz7pf 176 9 output output NN cord-349042-u9svz7pf 176 10 ; ; : cord-349042-u9svz7pf 176 11 because because IN cord-349042-u9svz7pf 176 12 the the DT cord-349042-u9svz7pf 176 13 RNA rna NN cord-349042-u9svz7pf 176 14 - - HYPH cord-349042-u9svz7pf 176 15 polymerase polymerase NN cord-349042-u9svz7pf 176 16 - - HYPH cord-349042-u9svz7pf 176 17 promoter promoter NN cord-349042-u9svz7pf 176 18 - - HYPH cord-349042-u9svz7pf 176 19 containing contain VBG cord-349042-u9svz7pf 176 20 DNAs dna NNS cord-349042-u9svz7pf 176 21 from from IN cord-349042-u9svz7pf 176 22 the the DT cord-349042-u9svz7pf 176 23 different different JJ cord-349042-u9svz7pf 176 24 detectors detector NNS cord-349042-u9svz7pf 176 25 are be VBP cord-349042-u9svz7pf 176 26 transcribed transcribe VBN cord-349042-u9svz7pf 176 27 , , , cord-349042-u9svz7pf 176 28 the the DT cord-349042-u9svz7pf 176 29 resultant resultant JJ cord-349042-u9svz7pf 176 30 aRNAs arna NNS cord-349042-u9svz7pf 176 31 can can MD cord-349042-u9svz7pf 176 32 be be VB cord-349042-u9svz7pf 176 33 used use VBN cord-349042-u9svz7pf 176 34 to to TO cord-349042-u9svz7pf 176 35 deduce deduce VB cord-349042-u9svz7pf 176 36 the the DT cord-349042-u9svz7pf 176 37 proteome proteome NN cord-349042-u9svz7pf 176 38 or or CC cord-349042-u9svz7pf 176 39 other other JJ cord-349042-u9svz7pf 176 40 chemicals chemical NNS cord-349042-u9svz7pf 176 41 on on IN cord-349042-u9svz7pf 176 42 the the DT cord-349042-u9svz7pf 176 43 basis basis NN cord-349042-u9svz7pf 176 44 of of IN cord-349042-u9svz7pf 176 45 the the DT cord-349042-u9svz7pf 176 46 specific specific JJ cord-349042-u9svz7pf 176 47 barcodes barcode NNS cord-349042-u9svz7pf 176 48 that that WDT cord-349042-u9svz7pf 176 49 are be VBP cord-349042-u9svz7pf 176 50 detected detect VBN cord-349042-u9svz7pf 176 51 . . . cord-349042-u9svz7pf 177 1 For for IN cord-349042-u9svz7pf 177 2 example example NN cord-349042-u9svz7pf 177 3 , , , cord-349042-u9svz7pf 177 4 it -PRON- PRP cord-349042-u9svz7pf 177 5 may may MD cord-349042-u9svz7pf 177 6 be be VB cord-349042-u9svz7pf 177 7 possible possible JJ cord-349042-u9svz7pf 177 8 to to TO cord-349042-u9svz7pf 177 9 use use VB cord-349042-u9svz7pf 177 10 APRA APRA NNP cord-349042-u9svz7pf 177 11 to to TO cord-349042-u9svz7pf 177 12 detect detect VB cord-349042-u9svz7pf 177 13 specific specific JJ cord-349042-u9svz7pf 177 14 RBP rbp NN cord-349042-u9svz7pf 177 15 - - HYPH cord-349042-u9svz7pf 177 16 associated associate VBN cord-349042-u9svz7pf 177 17 RNA RNA NNP cord-349042-u9svz7pf 177 18 cargoes cargo NNS cord-349042-u9svz7pf 177 19 with with IN cord-349042-u9svz7pf 177 20 unique unique JJ cord-349042-u9svz7pf 177 21 barcodes barcode NNS cord-349042-u9svz7pf 177 22 in in IN cord-349042-u9svz7pf 177 23 the the DT cord-349042-u9svz7pf 177 24 same same JJ cord-349042-u9svz7pf 177 25 cell cell NN cord-349042-u9svz7pf 177 26 where where WRB cord-349042-u9svz7pf 177 27 the the DT cord-349042-u9svz7pf 177 28 transcriptome transcriptome NN cord-349042-u9svz7pf 177 29 is be VBZ cord-349042-u9svz7pf 177 30 analyzed analyze VBN cord-349042-u9svz7pf 177 31 ( ( -LRB- cord-349042-u9svz7pf 177 32 both both DT cord-349042-u9svz7pf 177 33 will will MD cord-349042-u9svz7pf 177 34 be be VB cord-349042-u9svz7pf 177 35 linearly linearly RB cord-349042-u9svz7pf 177 36 amplified amplify VBN cord-349042-u9svz7pf 177 37 , , , cord-349042-u9svz7pf 177 38 but but CC cord-349042-u9svz7pf 177 39 they -PRON- PRP cord-349042-u9svz7pf 177 40 will will MD cord-349042-u9svz7pf 177 41 be be VB cord-349042-u9svz7pf 177 42 distinct distinct JJ cord-349042-u9svz7pf 177 43 and and CC cord-349042-u9svz7pf 177 44 distinguishable distinguishable JJ cord-349042-u9svz7pf 177 45 on on IN cord-349042-u9svz7pf 177 46 the the DT cord-349042-u9svz7pf 177 47 basis basis NN cord-349042-u9svz7pf 177 48 of of IN cord-349042-u9svz7pf 177 49 different different JJ cord-349042-u9svz7pf 177 50 sets set NNS cord-349042-u9svz7pf 177 51 of of IN cord-349042-u9svz7pf 177 52 barcodes barcode NNS cord-349042-u9svz7pf 177 53 ) ) -RRB- cord-349042-u9svz7pf 177 54 . . . cord-349042-u9svz7pf 178 1 This this DT cord-349042-u9svz7pf 178 2 would would MD cord-349042-u9svz7pf 178 3 facilitate facilitate VB cord-349042-u9svz7pf 178 4 analysis analysis NN cord-349042-u9svz7pf 178 5 of of IN cord-349042-u9svz7pf 178 6 the the DT cord-349042-u9svz7pf 178 7 dynamics dynamic NNS cord-349042-u9svz7pf 178 8 of of IN cord-349042-u9svz7pf 178 9 RNA RNA NNP cord-349042-u9svz7pf 178 10 movement movement NN cord-349042-u9svz7pf 178 11 between between IN cord-349042-u9svz7pf 178 12 different different JJ cord-349042-u9svz7pf 178 13 subcellular subcellular JJ cord-349042-u9svz7pf 178 14 compartments compartment NNS cord-349042-u9svz7pf 178 15 . . . cord-349042-u9svz7pf 179 1 As as IN cord-349042-u9svz7pf 179 2 another another DT cord-349042-u9svz7pf 179 3 example example NN cord-349042-u9svz7pf 179 4 , , , cord-349042-u9svz7pf 179 5 when when WRB cord-349042-u9svz7pf 179 6 coupled couple VBN cord-349042-u9svz7pf 179 7 with with IN cord-349042-u9svz7pf 179 8 the the DT cord-349042-u9svz7pf 179 9 use use NN cord-349042-u9svz7pf 179 10 of of IN cord-349042-u9svz7pf 179 11 antibodies antibody NNS cord-349042-u9svz7pf 179 12 to to IN cord-349042-u9svz7pf 179 13 specific specific JJ cord-349042-u9svz7pf 179 14 post post JJ cord-349042-u9svz7pf 179 15 - - JJ cord-349042-u9svz7pf 179 16 translational translational JJ cord-349042-u9svz7pf 179 17 modifications modification NNS cord-349042-u9svz7pf 179 18 such such JJ cord-349042-u9svz7pf 179 19 as as IN cord-349042-u9svz7pf 179 20 phosphorylation phosphorylation NN cord-349042-u9svz7pf 179 21 or or CC cord-349042-u9svz7pf 179 22 glycosylation glycosylation NN cord-349042-u9svz7pf 179 23 groups group NNS cord-349042-u9svz7pf 179 24 , , , cord-349042-u9svz7pf 179 25 IDAT IDAT NNP cord-349042-u9svz7pf 179 26 could could MD cord-349042-u9svz7pf 179 27 be be VB cord-349042-u9svz7pf 179 28 used use VBN cord-349042-u9svz7pf 179 29 to to TO cord-349042-u9svz7pf 179 30 assess assess VB cord-349042-u9svz7pf 179 31 the the DT cord-349042-u9svz7pf 179 32 biology biology NN cord-349042-u9svz7pf 179 33 and and CC cord-349042-u9svz7pf 179 34 dynamics dynamic NNS cord-349042-u9svz7pf 179 35 of of IN cord-349042-u9svz7pf 179 36 such such JJ cord-349042-u9svz7pf 179 37 protein protein NN cord-349042-u9svz7pf 179 38 modifications modification NNS cord-349042-u9svz7pf 179 39 at at IN cord-349042-u9svz7pf 179 40 the the DT cord-349042-u9svz7pf 179 41 same same JJ cord-349042-u9svz7pf 179 42 time time NN cord-349042-u9svz7pf 179 43 as as IN cord-349042-u9svz7pf 179 44 the the DT cord-349042-u9svz7pf 179 45 transcriptome transcriptome NN cord-349042-u9svz7pf 179 46 analysis analysis NN cord-349042-u9svz7pf 179 47 . . . cord-349042-u9svz7pf 180 1 Careful careful JJ cord-349042-u9svz7pf 180 2 use use NN cord-349042-u9svz7pf 180 3 of of IN cord-349042-u9svz7pf 180 4 barcodes barcode NNS cord-349042-u9svz7pf 180 5 in in IN cord-349042-u9svz7pf 180 6 LIANTIbased liantibased JJ cord-349042-u9svz7pf 180 7 DNA DNA NNP cord-349042-u9svz7pf 180 8 sequencing sequencing NN cord-349042-u9svz7pf 180 9 in in IN cord-349042-u9svz7pf 180 10 conjunction conjunction NN cord-349042-u9svz7pf 180 11 with with IN cord-349042-u9svz7pf 180 12 analysis analysis NN cord-349042-u9svz7pf 180 13 of of IN cord-349042-u9svz7pf 180 14 the the DT cord-349042-u9svz7pf 180 15 transcriptome transcriptome NN cord-349042-u9svz7pf 180 16 ( ( -LRB- cord-349042-u9svz7pf 180 17 using use VBG cord-349042-u9svz7pf 180 18 a a DT cord-349042-u9svz7pf 180 19 distinctly distinctly RB cord-349042-u9svz7pf 180 20 barcoded barcoded JJ cord-349042-u9svz7pf 180 21 oligo(dT)-T7 oligo(dt)-t7 JJ cord-349042-u9svz7pf 180 22 primer primer NN cord-349042-u9svz7pf 180 23 to to IN cord-349042-u9svz7pf 180 24 prime prime JJ cord-349042-u9svz7pf 180 25 amplification amplification NN cord-349042-u9svz7pf 180 26 of of IN cord-349042-u9svz7pf 180 27 the the DT cord-349042-u9svz7pf 180 28 poly(A poly(a JJ cord-349042-u9svz7pf 180 29 ) ) -RRB- cord-349042-u9svz7pf 180 30 RNA RNA NNP cord-349042-u9svz7pf 180 31 population population NN cord-349042-u9svz7pf 180 32 ) ) -RRB- cord-349042-u9svz7pf 180 33 would would MD cord-349042-u9svz7pf 180 34 allow allow VB cord-349042-u9svz7pf 180 35 analysis analysis NN cord-349042-u9svz7pf 180 36 of of IN cord-349042-u9svz7pf 180 37 both both DT cord-349042-u9svz7pf 180 38 genomic genomic JJ cord-349042-u9svz7pf 180 39 DNA dna NN cord-349042-u9svz7pf 180 40 and and CC cord-349042-u9svz7pf 180 41 the the DT cord-349042-u9svz7pf 180 42 transcriptome transcriptome NN cord-349042-u9svz7pf 180 43 in in IN cord-349042-u9svz7pf 180 44 the the DT cord-349042-u9svz7pf 180 45 same same JJ cord-349042-u9svz7pf 180 46 cell cell NN cord-349042-u9svz7pf 180 47 . . . cord-349042-u9svz7pf 181 1 One one PRP cord-349042-u9svz7pf 181 2 could could MD cord-349042-u9svz7pf 181 3 take take VB cord-349042-u9svz7pf 181 4 this this DT cord-349042-u9svz7pf 181 5 even even RB cord-349042-u9svz7pf 181 6 further far RBR cord-349042-u9svz7pf 181 7 by by IN cord-349042-u9svz7pf 181 8 multiplexing multiplexe VBG cord-349042-u9svz7pf 181 9 more more JJR cord-349042-u9svz7pf 181 10 than than IN cord-349042-u9svz7pf 181 11 two two CD cord-349042-u9svz7pf 181 12 of of IN cord-349042-u9svz7pf 181 13 these these DT cord-349042-u9svz7pf 181 14 analysis analysis NN cord-349042-u9svz7pf 181 15 procedures procedure NNS cord-349042-u9svz7pf 181 16 . . . cord-349042-u9svz7pf 182 1 For for IN cord-349042-u9svz7pf 182 2 example example NN cord-349042-u9svz7pf 182 3 , , , cord-349042-u9svz7pf 182 4 the the DT cord-349042-u9svz7pf 182 5 transcriptome transcriptome NN cord-349042-u9svz7pf 182 6 ( ( -LRB- cord-349042-u9svz7pf 182 7 assessed assess VBN cord-349042-u9svz7pf 182 8 via via IN cord-349042-u9svz7pf 182 9 the the DT cord-349042-u9svz7pf 182 10 aRNA arna NN cord-349042-u9svz7pf 182 11 procedure procedure NN cord-349042-u9svz7pf 182 12 ) ) -RRB- cord-349042-u9svz7pf 182 13 , , , cord-349042-u9svz7pf 182 14 genomic genomic JJ cord-349042-u9svz7pf 182 15 DNA dna NN cord-349042-u9svz7pf 182 16 ( ( -LRB- cord-349042-u9svz7pf 182 17 analyzed analyze VBN cord-349042-u9svz7pf 182 18 by by IN cord-349042-u9svz7pf 182 19 LIANTI LIANTI NNP cord-349042-u9svz7pf 182 20 ) ) -RRB- cord-349042-u9svz7pf 182 21 , , , cord-349042-u9svz7pf 182 22 and and CC cord-349042-u9svz7pf 182 23 protein protein NN cord-349042-u9svz7pf 182 24 ( ( -LRB- cord-349042-u9svz7pf 182 25 evaluated evaluate VBN cord-349042-u9svz7pf 182 26 by by IN cord-349042-u9svz7pf 182 27 IDAT IDAT NNP cord-349042-u9svz7pf 182 28 ) ) -RRB- cord-349042-u9svz7pf 182 29 could could MD cord-349042-u9svz7pf 182 30 all all DT cord-349042-u9svz7pf 182 31 be be VB cord-349042-u9svz7pf 182 32 analyzed analyze VBN cord-349042-u9svz7pf 182 33 in in IN cord-349042-u9svz7pf 182 34 the the DT cord-349042-u9svz7pf 182 35 same same JJ cord-349042-u9svz7pf 182 36 cell cell NN cord-349042-u9svz7pf 182 37 . . . cord-349042-u9svz7pf 183 1 Such such JJ cord-349042-u9svz7pf 183 2 combinatorial combinatorial JJ cord-349042-u9svz7pf 183 3 use use NN cord-349042-u9svz7pf 183 4 of of IN cord-349042-u9svz7pf 183 5 aRNA arna RB cord-349042-u9svz7pf 183 6 - - HYPH cord-349042-u9svz7pf 183 7 amplificationbased amplificationbase VBN cord-349042-u9svz7pf 183 8 methods method NNS cord-349042-u9svz7pf 183 9 should should MD cord-349042-u9svz7pf 183 10 enable enable VB cord-349042-u9svz7pf 183 11 the the DT cord-349042-u9svz7pf 183 12 quantitative quantitative JJ cord-349042-u9svz7pf 183 13 analysis analysis NN cord-349042-u9svz7pf 183 14 of of IN cord-349042-u9svz7pf 183 15 many many JJ cord-349042-u9svz7pf 183 16 cellular cellular JJ cord-349042-u9svz7pf 183 17 components component NNS cord-349042-u9svz7pf 183 18 and and CC cord-349042-u9svz7pf 183 19 their -PRON- PRP$ cord-349042-u9svz7pf 183 20 associated associate VBN cord-349042-u9svz7pf 183 21 biologies biology NNS cord-349042-u9svz7pf 183 22 from from IN cord-349042-u9svz7pf 183 23 a a DT cord-349042-u9svz7pf 183 24 single single JJ cord-349042-u9svz7pf 183 25 sample sample NN cord-349042-u9svz7pf 183 26 , , , cord-349042-u9svz7pf 183 27 down down IN cord-349042-u9svz7pf 183 28 to to IN cord-349042-u9svz7pf 183 29 and and CC cord-349042-u9svz7pf 183 30 including include VBG cord-349042-u9svz7pf 183 31 subregions subregion NNS cord-349042-u9svz7pf 183 32 of of IN cord-349042-u9svz7pf 183 33 a a DT cord-349042-u9svz7pf 183 34 single single JJ cord-349042-u9svz7pf 183 35 cell cell NN cord-349042-u9svz7pf 183 36 . . . cord-349042-u9svz7pf 184 1 Given give VBN cord-349042-u9svz7pf 184 2 the the DT cord-349042-u9svz7pf 184 3 flexibility flexibility NN cord-349042-u9svz7pf 184 4 of of IN cord-349042-u9svz7pf 184 5 the the DT cord-349042-u9svz7pf 184 6 technique technique NN cord-349042-u9svz7pf 184 7 and and CC cord-349042-u9svz7pf 184 8 the the DT cord-349042-u9svz7pf 184 9 ability ability NN cord-349042-u9svz7pf 184 10 of of IN cord-349042-u9svz7pf 184 11 different different JJ cord-349042-u9svz7pf 184 12 aRNA aRNA HYPH cord-349042-u9svz7pf 184 13 reporting report VBG cord-349042-u9svz7pf 184 14 methods method NNS cord-349042-u9svz7pf 184 15 to to TO cord-349042-u9svz7pf 184 16 barcode barcode VB cord-349042-u9svz7pf 184 17 different different JJ cord-349042-u9svz7pf 184 18 chemicals chemical NNS cord-349042-u9svz7pf 184 19 , , , cord-349042-u9svz7pf 184 20 it -PRON- PRP cord-349042-u9svz7pf 184 21 is be VBZ cord-349042-u9svz7pf 184 22 reasonable reasonable JJ cord-349042-u9svz7pf 184 23 to to TO cord-349042-u9svz7pf 184 24 presume presume VB cord-349042-u9svz7pf 184 25 that that IN cord-349042-u9svz7pf 184 26 linear linear NNP cord-349042-u9svz7pf 184 27 RNA RNA NNP cord-349042-u9svz7pf 184 28 amplification amplification NN cord-349042-u9svz7pf 184 29 will will MD cord-349042-u9svz7pf 184 30 continue continue VB cord-349042-u9svz7pf 184 31 to to TO cord-349042-u9svz7pf 184 32 be be VB cord-349042-u9svz7pf 184 33 used use VBN cord-349042-u9svz7pf 184 34 as as IN cord-349042-u9svz7pf 184 35 a a DT cord-349042-u9svz7pf 184 36 foundation foundation NN cord-349042-u9svz7pf 184 37 for for IN cord-349042-u9svz7pf 184 38 new new JJ cord-349042-u9svz7pf 184 39 technologies technology NNS cord-349042-u9svz7pf 184 40 , , , cord-349042-u9svz7pf 184 41 providing provide VBG cord-349042-u9svz7pf 184 42 deeper deep JJR cord-349042-u9svz7pf 184 43 understanding understanding NN cord-349042-u9svz7pf 184 44 of of IN cord-349042-u9svz7pf 184 45 the the DT cord-349042-u9svz7pf 184 46 multiple multiple JJ cord-349042-u9svz7pf 184 47 components component NNS cord-349042-u9svz7pf 184 48 and and CC cord-349042-u9svz7pf 184 49 processes process NNS cord-349042-u9svz7pf 184 50 of of IN cord-349042-u9svz7pf 184 51 cells cell NNS cord-349042-u9svz7pf 184 52 and and CC cord-349042-u9svz7pf 184 53 systems system NNS cord-349042-u9svz7pf 184 54 that that WDT cord-349042-u9svz7pf 184 55 contribute contribute VBP cord-349042-u9svz7pf 184 56 to to IN cord-349042-u9svz7pf 184 57 their -PRON- PRP$ cord-349042-u9svz7pf 184 58 function function NN cord-349042-u9svz7pf 184 59 . . . cord-349042-u9svz7pf 185 1 ACKNOWLEDGMENTS acknowledgment NNS cord-349042-u9svz7pf 186 1 This this DT cord-349042-u9svz7pf 186 2 work work NN cord-349042-u9svz7pf 186 3 was be VBD cord-349042-u9svz7pf 186 4 supported support VBN cord-349042-u9svz7pf 186 5 in in IN cord-349042-u9svz7pf 186 6 part part NN cord-349042-u9svz7pf 186 7 by by IN cord-349042-u9svz7pf 186 8 the the DT cord-349042-u9svz7pf 186 9 NIH NIH NNP cord-349042-u9svz7pf 186 10 ( ( -LRB- cord-349042-u9svz7pf 186 11 grants grant NNS cord-349042-u9svz7pf 186 12 U01MH098937 U01MH098937 NNP cord-349042-u9svz7pf 186 13 , , , cord-349042-u9svz7pf 186 14 GM10005 GM10005 NNP cord-349042-u9svz7pf 186 15 , , , cord-349042-u9svz7pf 186 16 MH110180 MH110180 NNP cord-349042-u9svz7pf 186 17 , , , cord-349042-u9svz7pf 186 18 and and CC cord-349042-u9svz7pf 186 19 MH098953 MH098953 NNS cord-349042-u9svz7pf 186 20 to to IN cord-349042-u9svz7pf 186 21 J.E. J.E. NNP cord-349042-u9svz7pf 186 22 ) ) -RRB- cord-349042-u9svz7pf 186 23 . . . cord-349042-u9svz7pf 187 1 We -PRON- PRP cord-349042-u9svz7pf 187 2 thank thank VBP cord-349042-u9svz7pf 187 3 J.-M. J.-M. NNP cord-349042-u9svz7pf 187 4 Eberwine Eberwine NNP cord-349042-u9svz7pf 187 5 and and CC cord-349042-u9svz7pf 187 6 L. L. NNP cord-349042-u9svz7pf 187 7 Hoban Hoban NNP cord-349042-u9svz7pf 187 8 for for IN cord-349042-u9svz7pf 187 9 editing editing NN cord-349042-u9svz7pf 187 10 . . . cord-349042-u9svz7pf 188 1 We -PRON- PRP cord-349042-u9svz7pf 188 2 also also RB cord-349042-u9svz7pf 188 3 thank thank VBP cord-349042-u9svz7pf 188 4 our -PRON- PRP$ cord-349042-u9svz7pf 188 5 many many JJ cord-349042-u9svz7pf 188 6 colleagues colleague NNS cord-349042-u9svz7pf 188 7 who who WP cord-349042-u9svz7pf 188 8 , , , cord-349042-u9svz7pf 188 9 over over IN cord-349042-u9svz7pf 188 10 the the DT cord-349042-u9svz7pf 188 11 years year NNS cord-349042-u9svz7pf 188 12 , , , cord-349042-u9svz7pf 188 13 have have VBP cord-349042-u9svz7pf 188 14 contributed contribute VBN cord-349042-u9svz7pf 188 15 their -PRON- PRP$ cord-349042-u9svz7pf 188 16 intellect intellect NN cord-349042-u9svz7pf 188 17 and and CC cord-349042-u9svz7pf 188 18 diligence diligence NN cord-349042-u9svz7pf 188 19 in in IN cord-349042-u9svz7pf 188 20 working work VBG cord-349042-u9svz7pf 188 21 with with IN cord-349042-u9svz7pf 188 22 us -PRON- PRP cord-349042-u9svz7pf 188 23 to to TO cord-349042-u9svz7pf 188 24 develop develop VB cord-349042-u9svz7pf 188 25 and and CC cord-349042-u9svz7pf 188 26 refine refine VB cord-349042-u9svz7pf 188 27 these these DT cord-349042-u9svz7pf 188 28 methods method NNS cord-349042-u9svz7pf 188 29 . . . cord-349042-u9svz7pf 189 1 AUTHOR author NN cord-349042-u9svz7pf 189 2 CONTRIBUTIONS CONTRIBUTIONS NNP cord-349042-u9svz7pf 189 3 J.L. J.L. NNP cord-349042-u9svz7pf 189 4 and and CC cord-349042-u9svz7pf 189 5 J.E. J.E. NNP cord-349042-u9svz7pf 189 6 wrote write VBD cord-349042-u9svz7pf 189 7 , , , cord-349042-u9svz7pf 189 8 reviewed review VBN cord-349042-u9svz7pf 189 9 , , , cord-349042-u9svz7pf 189 10 and and CC cord-349042-u9svz7pf 189 11 edited edit VBD cord-349042-u9svz7pf 189 12 the the DT cord-349042-u9svz7pf 189 13 manuscript manuscript NN cord-349042-u9svz7pf 189 14 . . . cord-349042-u9svz7pf 190 1 The the DT cord-349042-u9svz7pf 190 2 authors author NNS cord-349042-u9svz7pf 190 3 declare declare VBP cord-349042-u9svz7pf 190 4 no no DT cord-349042-u9svz7pf 190 5 competing compete VBG cord-349042-u9svz7pf 190 6 interests interest NNS cord-349042-u9svz7pf 190 7 . . . cord-349042-u9svz7pf 191 1 The the DT cord-349042-u9svz7pf 191 2 ability ability NN cord-349042-u9svz7pf 191 3 to to TO cord-349042-u9svz7pf 191 4 detect detect VB cord-349042-u9svz7pf 191 5 antigens antigen NNS cord-349042-u9svz7pf 191 6 immunologically immunologically RB cord-349042-u9svz7pf 191 7 is be VBZ cord-349042-u9svz7pf 191 8 often often RB cord-349042-u9svz7pf 191 9 limited limit VBN cord-349042-u9svz7pf 191 10 by by IN cord-349042-u9svz7pf 191 11 the the DT cord-349042-u9svz7pf 191 12 low low JJ cord-349042-u9svz7pf 191 13 affinity affinity NN cord-349042-u9svz7pf 191 14 of of IN cord-349042-u9svz7pf 191 15 many many JJ cord-349042-u9svz7pf 191 16 antibodies antibody NNS cord-349042-u9svz7pf 191 17 , , , cord-349042-u9svz7pf 191 18 the the DT cord-349042-u9svz7pf 191 19 limiting limit VBG cord-349042-u9svz7pf 191 20 amount amount NN cord-349042-u9svz7pf 191 21 of of IN cord-349042-u9svz7pf 191 22 many many JJ cord-349042-u9svz7pf 191 23 antigens antigen NNS cord-349042-u9svz7pf 191 24 , , , cord-349042-u9svz7pf 191 25 and and CC cord-349042-u9svz7pf 191 26 the the DT cord-349042-u9svz7pf 191 27 high high JJ cord-349042-u9svz7pf 191 28 background background NN cord-349042-u9svz7pf 191 29 often often RB cord-349042-u9svz7pf 191 30 associated associate VBN cord-349042-u9svz7pf 191 31 with with IN cord-349042-u9svz7pf 191 32 highsensitivity highsensitivity NN cord-349042-u9svz7pf 191 33 enzymatic enzymatic JJ cord-349042-u9svz7pf 191 34 or or CC cord-349042-u9svz7pf 191 35 fluorescence fluorescence NN cord-349042-u9svz7pf 191 36 detection detection NN cord-349042-u9svz7pf 191 37 methods method NNS cord-349042-u9svz7pf 191 38 . . . cord-349042-u9svz7pf 192 1 These these DT cord-349042-u9svz7pf 192 2 issues issue NNS cord-349042-u9svz7pf 192 3 can can MD cord-349042-u9svz7pf 192 4 be be VB cord-349042-u9svz7pf 192 5 overcome overcome VBN cord-349042-u9svz7pf 192 6 through through IN cord-349042-u9svz7pf 192 7 the the DT cord-349042-u9svz7pf 192 8 use use NN cord-349042-u9svz7pf 192 9 of of IN cord-349042-u9svz7pf 192 10 aRNA arna NN cord-349042-u9svz7pf 192 11 amplification amplification NN cord-349042-u9svz7pf 192 12 for for IN cord-349042-u9svz7pf 192 13 antigen antigen NN cord-349042-u9svz7pf 192 14 detection detection NN cord-349042-u9svz7pf 192 15 rather rather RB cord-349042-u9svz7pf 192 16 than than IN cord-349042-u9svz7pf 192 17 the the DT cord-349042-u9svz7pf 192 18 standard standard JJ cord-349042-u9svz7pf 192 19 fluorescence fluorescence NN cord-349042-u9svz7pf 192 20 detection detection NN cord-349042-u9svz7pf 192 21 , , , cord-349042-u9svz7pf 192 22 as as IN cord-349042-u9svz7pf 192 23 the the DT cord-349042-u9svz7pf 192 24 protein protein NN cord-349042-u9svz7pf 192 25 - - HYPH cord-349042-u9svz7pf 192 26 detection detection NN cord-349042-u9svz7pf 192 27 sensitivity sensitivity NN cord-349042-u9svz7pf 192 28 can can MD cord-349042-u9svz7pf 192 29 be be VB cord-349042-u9svz7pf 192 30 increased increase VBN cord-349042-u9svz7pf 192 31 ~10 ~10 . cord-349042-u9svz7pf 193 1 9 9 LS cord-349042-u9svz7pf 193 2 -fold -fold NN cord-349042-u9svz7pf 193 3 over over IN cord-349042-u9svz7pf 193 4 that that DT cord-349042-u9svz7pf 193 5 of of IN cord-349042-u9svz7pf 193 6 conventional conventional JJ cord-349042-u9svz7pf 193 7 ELISA ELISA NNP cord-349042-u9svz7pf 193 8 methods method NNS cord-349042-u9svz7pf 193 9 . . . cord-349042-u9svz7pf 194 1 This this DT cord-349042-u9svz7pf 194 2 aRNA arna NN cord-349042-u9svz7pf 194 3 proteindetection proteindetection NN cord-349042-u9svz7pf 194 4 technique technique NN cord-349042-u9svz7pf 194 5 is be VBZ cord-349042-u9svz7pf 194 6 termed term VBN cord-349042-u9svz7pf 194 7 immunodetection immunodetection NN cord-349042-u9svz7pf 194 8 amplified amplify VBN cord-349042-u9svz7pf 194 9 by by IN cord-349042-u9svz7pf 194 10 T7 T7 NNP cord-349042-u9svz7pf 194 11 RNA RNA NNP cord-349042-u9svz7pf 194 12 polymerase polymerase NN cord-349042-u9svz7pf 194 13 ( ( -LRB- cord-349042-u9svz7pf 194 14 IDAT IDAT NNP cord-349042-u9svz7pf 194 15 ) ) -RRB- cord-349042-u9svz7pf 194 16 ( ( -LRB- cord-349042-u9svz7pf 194 17 Fig Fig NNP cord-349042-u9svz7pf 194 18 . . . cord-349042-u9svz7pf 194 19 2c 2c NNP cord-349042-u9svz7pf 194 20 ) ) -RRB- cord-349042-u9svz7pf 194 21 . . . cord-349042-u9svz7pf 195 1 Zhang Zhang NNP cord-349042-u9svz7pf 195 2 et et NNP cord-349042-u9svz7pf 195 3 al al NNP cord-349042-u9svz7pf 195 4 . . . cord-349042-u9svz7pf 196 1 45 45 CD cord-349042-u9svz7pf 196 2 developed develop VBD cord-349042-u9svz7pf 196 3 a a DT cord-349042-u9svz7pf 196 4 method method NN cord-349042-u9svz7pf 196 5 to to TO cord-349042-u9svz7pf 196 6 facilitate facilitate VB cord-349042-u9svz7pf 196 7 aRNA arna NN cord-349042-u9svz7pf 196 8 detection detection NN cord-349042-u9svz7pf 196 9 of of IN cord-349042-u9svz7pf 196 10 antibody antibody NN cord-349042-u9svz7pf 196 11 - - HYPH cord-349042-u9svz7pf 196 12 antigen antigen NN cord-349042-u9svz7pf 196 13 interactions interaction NNS cord-349042-u9svz7pf 196 14 by by IN cord-349042-u9svz7pf 196 15 covalently covalently RB cord-349042-u9svz7pf 196 16 attaching attach VBG cord-349042-u9svz7pf 196 17 a a DT cord-349042-u9svz7pf 196 18 double double RB cord-349042-u9svz7pf 196 19 - - HYPH cord-349042-u9svz7pf 196 20 stranded strand VBN cord-349042-u9svz7pf 196 21 cDNA cdna NN cord-349042-u9svz7pf 196 22 that that WDT cord-349042-u9svz7pf 196 23 contains contain VBZ cord-349042-u9svz7pf 196 24 a a DT cord-349042-u9svz7pf 196 25 T7 T7 NNP cord-349042-u9svz7pf 196 26 RNA RNA NNP cord-349042-u9svz7pf 196 27 polymerase polymerase NN cord-349042-u9svz7pf 196 28 promoter promoter NN cord-349042-u9svz7pf 196 29 in in IN cord-349042-u9svz7pf 196 30 front front NN cord-349042-u9svz7pf 196 31 of of IN cord-349042-u9svz7pf 196 32 a a DT cord-349042-u9svz7pf 196 33 reporter reporter NN cord-349042-u9svz7pf 196 34 sequence sequence NN cord-349042-u9svz7pf 196 35 to to IN cord-349042-u9svz7pf 196 36 a a DT cord-349042-u9svz7pf 196 37 specific specific JJ cord-349042-u9svz7pf 196 38 antibody antibody NN cord-349042-u9svz7pf 196 39 . . . cord-349042-u9svz7pf 197 1 After after IN cord-349042-u9svz7pf 197 2 immunostaining immunostaine VBG cord-349042-u9svz7pf 197 3 with with IN cord-349042-u9svz7pf 197 4 the the DT cord-349042-u9svz7pf 197 5 IDAT IDAT NNP cord-349042-u9svz7pf 197 6 antibody antibody NN cord-349042-u9svz7pf 197 7 , , , cord-349042-u9svz7pf 197 8 RNA RNA NNP cord-349042-u9svz7pf 197 9 polymerase polymerase NN cord-349042-u9svz7pf 197 10 is be VBZ cord-349042-u9svz7pf 197 11 added add VBN cord-349042-u9svz7pf 197 12 to to IN cord-349042-u9svz7pf 197 13 the the DT cord-349042-u9svz7pf 197 14 antigenantibody antigenantibody NN cord-349042-u9svz7pf 197 15 complex complex JJ cord-349042-u9svz7pf 197 16 to to TO cord-349042-u9svz7pf 197 17 linearly linearly RB cord-349042-u9svz7pf 197 18 amplify amplify VB cord-349042-u9svz7pf 197 19 RNA RNA NNP cord-349042-u9svz7pf 197 20 from from IN cord-349042-u9svz7pf 197 21 the the DT cord-349042-u9svz7pf 197 22 cDNA cdna NN cord-349042-u9svz7pf 197 23 that that WDT cord-349042-u9svz7pf 197 24 is be VBZ cord-349042-u9svz7pf 197 25 conjugated conjugate VBN cord-349042-u9svz7pf 197 26 to to IN cord-349042-u9svz7pf 197 27 the the DT cord-349042-u9svz7pf 197 28 antibody antibody NN cord-349042-u9svz7pf 197 29 . . . cord-349042-u9svz7pf 198 1 Because because IN cord-349042-u9svz7pf 198 2 the the DT cord-349042-u9svz7pf 198 3 amplification amplification NN cord-349042-u9svz7pf 198 4 is be VBZ cord-349042-u9svz7pf 198 5 linear linear JJ cord-349042-u9svz7pf 198 6 , , , cord-349042-u9svz7pf 198 7 the the DT cord-349042-u9svz7pf 198 8 yield yield NN cord-349042-u9svz7pf 198 9 of of IN cord-349042-u9svz7pf 198 10 aRNA arna NN cord-349042-u9svz7pf 198 11 is be VBZ cord-349042-u9svz7pf 198 12 a a DT cord-349042-u9svz7pf 198 13 direct direct JJ cord-349042-u9svz7pf 198 14 measure measure NN cord-349042-u9svz7pf 198 15 of of IN cord-349042-u9svz7pf 198 16 the the DT cord-349042-u9svz7pf 198 17 amount amount NN cord-349042-u9svz7pf 198 18 of of IN cord-349042-u9svz7pf 198 19 antibodyantigen antibodyantigen NN cord-349042-u9svz7pf 198 20 interaction interaction NN cord-349042-u9svz7pf 198 21 . . . cord-349042-u9svz7pf 199 1 A a DT cord-349042-u9svz7pf 199 2 fluorescence fluorescence NN cord-349042-u9svz7pf 199 3 - - HYPH cord-349042-u9svz7pf 199 4 based base VBN cord-349042-u9svz7pf 199 5 modification modification NN cord-349042-u9svz7pf 199 6 of of IN cord-349042-u9svz7pf 199 7 IDAT IDAT NNP cord-349042-u9svz7pf 199 8 subsequently subsequently RB cord-349042-u9svz7pf 199 9 developed develop VBN cord-349042-u9svz7pf 199 10 by by IN cord-349042-u9svz7pf 199 11 the the DT cord-349042-u9svz7pf 199 12 same same JJ cord-349042-u9svz7pf 199 13 group group NN cord-349042-u9svz7pf 199 14 , , , cord-349042-u9svz7pf 199 15 termed term VBN cord-349042-u9svz7pf 199 16 fluorescent fluorescent JJ cord-349042-u9svz7pf 199 17 amplification amplification NN cord-349042-u9svz7pf 199 18 catalyzed catalyze VBN cord-349042-u9svz7pf 199 19 by by IN cord-349042-u9svz7pf 199 20 T7 t7 NN cord-349042-u9svz7pf 199 21 polymerase polymerase NN cord-349042-u9svz7pf 199 22 technique technique NN cord-349042-u9svz7pf 199 23 ( ( -LRB- cord-349042-u9svz7pf 199 24 FACTT FACTT NNP cord-349042-u9svz7pf 199 25 ) ) -RRB- cord-349042-u9svz7pf 199 26 , , , cord-349042-u9svz7pf 199 27 was be VBD cord-349042-u9svz7pf 199 28 successfully successfully RB cord-349042-u9svz7pf 199 29 used use VBN cord-349042-u9svz7pf 199 30 to to TO cord-349042-u9svz7pf 199 31 analyze analyze VB cord-349042-u9svz7pf 199 32 low low JJ cord-349042-u9svz7pf 199 33 - - HYPH cord-349042-u9svz7pf 199 34 abundance abundance NN cord-349042-u9svz7pf 199 35 oncogene oncogene NN cord-349042-u9svz7pf 199 36 proteins protein NNS cord-349042-u9svz7pf 199 37 in in IN cord-349042-u9svz7pf 199 38 human human JJ cord-349042-u9svz7pf 199 39 serum serum NN cord-349042-u9svz7pf 199 40 46 46 CD cord-349042-u9svz7pf 199 41 . . . cord-349042-u9svz7pf 200 1 Rather rather RB cord-349042-u9svz7pf 200 2 than than IN cord-349042-u9svz7pf 200 3 using use VBG cord-349042-u9svz7pf 200 4 chemical chemical NN cord-349042-u9svz7pf 200 5 crosslinking crosslinking NN cord-349042-u9svz7pf 200 6 of of IN cord-349042-u9svz7pf 200 7 the the DT cord-349042-u9svz7pf 200 8 amplification amplification NN cord-349042-u9svz7pf 200 9 primer primer NN cord-349042-u9svz7pf 200 10 to to IN cord-349042-u9svz7pf 200 11 the the DT cord-349042-u9svz7pf 200 12 antibody antibody NN cord-349042-u9svz7pf 200 13 , , , cord-349042-u9svz7pf 200 14 FACTT FACTT NNP cord-349042-u9svz7pf 200 15 uses use VBZ cord-349042-u9svz7pf 200 16 streptavidin streptavidin NNS cord-349042-u9svz7pf 200 17 - - HYPH cord-349042-u9svz7pf 200 18 labeled label VBN cord-349042-u9svz7pf 200 19 antibodies antibody NNS cord-349042-u9svz7pf 200 20 to to TO cord-349042-u9svz7pf 200 21 bind bind VB cord-349042-u9svz7pf 200 22 the the DT cord-349042-u9svz7pf 200 23 biotinylated biotinylated JJ cord-349042-u9svz7pf 200 24 amplification amplification NN cord-349042-u9svz7pf 200 25 target target NN cord-349042-u9svz7pf 200 26 sequence sequence NN cord-349042-u9svz7pf 200 27 . . . cord-349042-u9svz7pf 201 1 Because because IN cord-349042-u9svz7pf 201 2 streptavidin streptavidin NNP cord-349042-u9svz7pf 201 3 binds bind VBZ cord-349042-u9svz7pf 201 4 many many JJ cord-349042-u9svz7pf 201 5 biotinylated biotinylated JJ cord-349042-u9svz7pf 201 6 molecules molecule NNS cord-349042-u9svz7pf 201 7 with with IN cord-349042-u9svz7pf 201 8 higher high JJR cord-349042-u9svz7pf 201 9 affinity affinity NN cord-349042-u9svz7pf 201 10 than than IN cord-349042-u9svz7pf 201 11 antibody antibody NN cord-349042-u9svz7pf 201 12 - - HYPH cord-349042-u9svz7pf 201 13 antigen antigen NN cord-349042-u9svz7pf 201 14 binding binding NN cord-349042-u9svz7pf 201 15 , , , cord-349042-u9svz7pf 201 16 the the DT cord-349042-u9svz7pf 201 17 sensitivity sensitivity NN cord-349042-u9svz7pf 201 18 of of IN cord-349042-u9svz7pf 201 19 FACTT FACTT NNP cord-349042-u9svz7pf 201 20 is be VBZ cord-349042-u9svz7pf 201 21 greater great JJR cord-349042-u9svz7pf 201 22 than than IN cord-349042-u9svz7pf 201 23 that that DT cord-349042-u9svz7pf 201 24 of of IN cord-349042-u9svz7pf 201 25 IDAT IDAT NNP cord-349042-u9svz7pf 201 26 . . . cord-349042-u9svz7pf 202 1 RiboGreen RiboGreen NNP cord-349042-u9svz7pf 202 2 , , , cord-349042-u9svz7pf 202 3 a a DT cord-349042-u9svz7pf 202 4 fluorescent fluorescent JJ cord-349042-u9svz7pf 202 5 RNA RNA NNP cord-349042-u9svz7pf 202 6 intercalating intercalating NN cord-349042-u9svz7pf 202 7 dye dye NN cord-349042-u9svz7pf 202 8 , , , cord-349042-u9svz7pf 202 9 can can MD cord-349042-u9svz7pf 202 10 be be VB cord-349042-u9svz7pf 202 11 used use VBN cord-349042-u9svz7pf 202 12 to to TO cord-349042-u9svz7pf 202 13 quantify quantify VB cord-349042-u9svz7pf 202 14 the the DT cord-349042-u9svz7pf 202 15 yield yield NN cord-349042-u9svz7pf 202 16 of of IN cord-349042-u9svz7pf 202 17 amplified amplify VBN cord-349042-u9svz7pf 202 18 RNA RNA NNP cord-349042-u9svz7pf 202 19 . . . cord-349042-u9svz7pf 203 1 IDAT IDAT NNP cord-349042-u9svz7pf 203 2 and and CC cord-349042-u9svz7pf 203 3 FACTT FACTT NNP cord-349042-u9svz7pf 203 4 should should MD cord-349042-u9svz7pf 203 5 be be VB cord-349042-u9svz7pf 203 6 adaptable adaptable JJ cord-349042-u9svz7pf 203 7 to to IN cord-349042-u9svz7pf 203 8 automation automation NN cord-349042-u9svz7pf 203 9 and and CC cord-349042-u9svz7pf 203 10 high high JJ cord-349042-u9svz7pf 203 11 - - HYPH cord-349042-u9svz7pf 203 12 throughput throughput NN cord-349042-u9svz7pf 203 13 protein protein NN cord-349042-u9svz7pf 203 14 screening screening NN cord-349042-u9svz7pf 203 15 . . . cord-349042-u9svz7pf 204 1 The the DT cord-349042-u9svz7pf 204 2 coordinated coordinated JJ cord-349042-u9svz7pf 204 3 activity activity NN cord-349042-u9svz7pf 204 4 of of IN cord-349042-u9svz7pf 204 5 multiple multiple JJ cord-349042-u9svz7pf 204 6 cells cell NNS cord-349042-u9svz7pf 204 7 gives give VBZ cord-349042-u9svz7pf 204 8 rise rise NN cord-349042-u9svz7pf 204 9 to to IN cord-349042-u9svz7pf 204 10 tissuespecific tissuespecific JJ cord-349042-u9svz7pf 204 11 functioning functioning NN cord-349042-u9svz7pf 204 12 . . . cord-349042-u9svz7pf 205 1 In in IN cord-349042-u9svz7pf 205 2 this this DT cord-349042-u9svz7pf 205 3 context context NN cord-349042-u9svz7pf 205 4 the the DT cord-349042-u9svz7pf 205 5 functional functional JJ cord-349042-u9svz7pf 205 6 cell cell NN cord-349042-u9svz7pf 205 7 type type NN cord-349042-u9svz7pf 205 8 is be VBZ cord-349042-u9svz7pf 205 9 of of IN cord-349042-u9svz7pf 205 10 utmost utmost JJ cord-349042-u9svz7pf 205 11 biological biological JJ cord-349042-u9svz7pf 205 12 importance importance NN cord-349042-u9svz7pf 205 13 . . . cord-349042-u9svz7pf 206 1 Quantitation quantitation NN cord-349042-u9svz7pf 206 2 of of IN cord-349042-u9svz7pf 206 3 the the DT cord-349042-u9svz7pf 206 4 subcellular subcellular JJ cord-349042-u9svz7pf 206 5 components component NNS cord-349042-u9svz7pf 206 6 and and CC cord-349042-u9svz7pf 206 7 processes process NNS cord-349042-u9svz7pf 206 8 that that WDT cord-349042-u9svz7pf 206 9 give give VBP cord-349042-u9svz7pf 206 10 rise rise NN cord-349042-u9svz7pf 206 11 to to IN cord-349042-u9svz7pf 206 12 cellular cellular JJ cord-349042-u9svz7pf 206 13 functions function NNS cord-349042-u9svz7pf 206 14 such such JJ cord-349042-u9svz7pf 206 15 as as IN cord-349042-u9svz7pf 206 16 genomic genomic JJ cord-349042-u9svz7pf 206 17 DNA dna NN cord-349042-u9svz7pf 206 18 sequence sequence NN cord-349042-u9svz7pf 206 19 variation variation NN cord-349042-u9svz7pf 206 20 , , , cord-349042-u9svz7pf 206 21 gene gene NN cord-349042-u9svz7pf 206 22 expression expression NN cord-349042-u9svz7pf 206 23 , , , cord-349042-u9svz7pf 206 24 methylation methylation NN cord-349042-u9svz7pf 206 25 , , , cord-349042-u9svz7pf 206 26 and and CC cord-349042-u9svz7pf 206 27 the the DT cord-349042-u9svz7pf 206 28 creation creation NN cord-349042-u9svz7pf 206 29 of of IN cord-349042-u9svz7pf 206 30 metabolites metabolite NNS cord-349042-u9svz7pf 206 31 and and CC cord-349042-u9svz7pf 206 32 proteins protein NNS cord-349042-u9svz7pf 206 33 will will MD cord-349042-u9svz7pf 206 34 improve improve VB cord-349042-u9svz7pf 206 35 the the DT cord-349042-u9svz7pf 206 36 overall overall JJ cord-349042-u9svz7pf 206 37 understanding understanding NN cord-349042-u9svz7pf 206 38 of of IN cord-349042-u9svz7pf 206 39 hierarchical hierarchical JJ cord-349042-u9svz7pf 206 40 cellular cellular JJ cord-349042-u9svz7pf 206 41 regulation regulation NN cord-349042-u9svz7pf 206 42 and and CC cord-349042-u9svz7pf 206 43 allow allow VB cord-349042-u9svz7pf 206 44 the the DT cord-349042-u9svz7pf 206 45 construction construction NN cord-349042-u9svz7pf 206 46 of of IN cord-349042-u9svz7pf 206 47 an an DT cord-349042-u9svz7pf 206 48 ' ' `` cord-349042-u9svz7pf 206 49 architecture architecture NN cord-349042-u9svz7pf 206 50 of of IN cord-349042-u9svz7pf 206 51 cellular cellular JJ cord-349042-u9svz7pf 206 52 phenotype phenotype NN cord-349042-u9svz7pf 206 53 ' ' '' cord-349042-u9svz7pf 206 54 . . . cord-349042-u9svz7pf 207 1 Such such PDT cord-349042-u9svz7pf 207 2 an an DT cord-349042-u9svz7pf 207 3 architecture architecture NN cord-349042-u9svz7pf 207 4 would would MD cord-349042-u9svz7pf 207 5 enable enable VB cord-349042-u9svz7pf 207 6 the the DT cord-349042-u9svz7pf 207 7 codetermination codetermination NN cord-349042-u9svz7pf 207 8 of of IN cord-349042-u9svz7pf 207 9 genomic genomic JJ cord-349042-u9svz7pf 207 10 DNA dna NN cord-349042-u9svz7pf 207 11 sequence sequence NN cord-349042-u9svz7pf 207 12 and and CC cord-349042-u9svz7pf 207 13 expression expression NN cord-349042-u9svz7pf 207 14 state state NN cord-349042-u9svz7pf 207 15 , , , cord-349042-u9svz7pf 207 16 as as RB cord-349042-u9svz7pf 207 17 well well RB cord-349042-u9svz7pf 207 18 as as IN cord-349042-u9svz7pf 207 19 allow allow VB cord-349042-u9svz7pf 207 20 RNA RNA NNP cord-349042-u9svz7pf 207 21 and and CC cord-349042-u9svz7pf 207 22 protein protein NN cord-349042-u9svz7pf 207 23 abundances abundance NNS cord-349042-u9svz7pf 207 24 to to TO cord-349042-u9svz7pf 207 25 complement complement VB cord-349042-u9svz7pf 207 26 each each DT cord-349042-u9svz7pf 207 27 other other JJ cord-349042-u9svz7pf 207 28 , , , cord-349042-u9svz7pf 207 29 with with IN cord-349042-u9svz7pf 207 30 variations variation NNS cord-349042-u9svz7pf 207 31 in in IN cord-349042-u9svz7pf 207 32 dynamics dynamic NNS cord-349042-u9svz7pf 207 33 and and CC cord-349042-u9svz7pf 207 34 absolute absolute JJ cord-349042-u9svz7pf 207 35 and and CC cord-349042-u9svz7pf 207 36 relative relative JJ cord-349042-u9svz7pf 207 37 amounts amount NNS cord-349042-u9svz7pf 207 38 of of IN cord-349042-u9svz7pf 207 39 each each DT cord-349042-u9svz7pf 207 40 cellular cellular JJ cord-349042-u9svz7pf 207 41 chemical chemical NN cord-349042-u9svz7pf 207 42 resulting result VBG cord-349042-u9svz7pf 207 43 in in IN cord-349042-u9svz7pf 207 44 specific specific JJ cord-349042-u9svz7pf 207 45 celltype celltype NN cord-349042-u9svz7pf 207 46 designations designation NNS cord-349042-u9svz7pf 207 47 . . . cord-349042-u9svz7pf 208 1 This this DT cord-349042-u9svz7pf 208 2 will will MD cord-349042-u9svz7pf 208 3 be be VB cord-349042-u9svz7pf 208 4 possible possible JJ cord-349042-u9svz7pf 208 5 only only RB cord-349042-u9svz7pf 208 6 when when WRB cord-349042-u9svz7pf 208 7 all all DT cord-349042-u9svz7pf 208 8 of of IN cord-349042-u9svz7pf 208 9 these these DT cord-349042-u9svz7pf 208 10 cellular cellular JJ cord-349042-u9svz7pf 208 11 constituents constituent NNS cord-349042-u9svz7pf 208 12 can can MD cord-349042-u9svz7pf 208 13 be be VB cord-349042-u9svz7pf 208 14 quantified quantify VBN cord-349042-u9svz7pf 208 15 in in IN cord-349042-u9svz7pf 208 16 the the DT cord-349042-u9svz7pf 208 17 same same JJ cord-349042-u9svz7pf 208 18 sample sample NN cord-349042-u9svz7pf 208 19 . . . cord-349042-u9svz7pf 209 1 The the DT cord-349042-u9svz7pf 209 2 easy easy JJ cord-349042-u9svz7pf 209 3 adaptation adaptation NN cord-349042-u9svz7pf 209 4 of of IN cord-349042-u9svz7pf 209 5 the the DT cord-349042-u9svz7pf 209 6 aRNA arna NN cord-349042-u9svz7pf 209 7 procedure procedure NN cord-349042-u9svz7pf 209 8 to to IN cord-349042-u9svz7pf 209 9 the the DT cord-349042-u9svz7pf 209 10 analysis analysis NN cord-349042-u9svz7pf 209 11 of of IN cord-349042-u9svz7pf 209 12 different different JJ cord-349042-u9svz7pf 209 13 subcellular subcellular JJ cord-349042-u9svz7pf 209 14 chemicals chemical NNS cord-349042-u9svz7pf 209 15 and and CC cord-349042-u9svz7pf 209 16 biologies biology NNS cord-349042-u9svz7pf 209 17 as as IN cord-349042-u9svz7pf 209 18 illustrated illustrate VBN cord-349042-u9svz7pf 209 19 above above IN cord-349042-u9svz7pf 209 20 gives give VBZ cord-349042-u9svz7pf 209 21 rise rise NN cord-349042-u9svz7pf 209 22 to to IN cord-349042-u9svz7pf 209 23 the the DT cord-349042-u9svz7pf 209 24 possibility possibility NN cord-349042-u9svz7pf 209 25 of of IN cord-349042-u9svz7pf 209 26 using use VBG cord-349042-u9svz7pf 209 27 this this DT cord-349042-u9svz7pf 209 28 methodology methodology NN cord-349042-u9svz7pf 209 29 in in IN cord-349042-u9svz7pf 209 30 a a DT cord-349042-u9svz7pf 209 31 multiplexed multiplexed JJ cord-349042-u9svz7pf 209 32 manner manner NN cord-349042-u9svz7pf 209 33 to to TO cord-349042-u9svz7pf 209 34 simultaneously simultaneously RB cord-349042-u9svz7pf 209 35 analyze analyze VB cord-349042-u9svz7pf 209 36 multiple multiple JJ cord-349042-u9svz7pf 209 37 subcellular subcellular JJ cord-349042-u9svz7pf 209 38 chemicals chemical NNS cord-349042-u9svz7pf 209 39 within within IN cord-349042-u9svz7pf 209 40 a a DT cord-349042-u9svz7pf 209 41 cell cell NN cord-349042-u9svz7pf 209 42 . . . cord-349042-u9svz7pf 210 1 Such such JJ cord-349042-u9svz7pf 210 2 multiplexing multiplexing NN cord-349042-u9svz7pf 210 3 is be VBZ cord-349042-u9svz7pf 210 4 currently currently RB cord-349042-u9svz7pf 210 5 possible possible JJ cord-349042-u9svz7pf 210 6 for for IN cord-349042-u9svz7pf 210 7 two two CD cord-349042-u9svz7pf 210 8 chemicals chemical NNS cord-349042-u9svz7pf 210 9 at at IN cord-349042-u9svz7pf 210 10 a a DT cord-349042-u9svz7pf 210 11 time time NN cord-349042-u9svz7pf 210 12 ( ( -LRB- cord-349042-u9svz7pf 210 13 for for IN cord-349042-u9svz7pf 210 14 example example NN cord-349042-u9svz7pf 210 15 , , , cord-349042-u9svz7pf 210 16 transcriptome transcriptome NN cord-349042-u9svz7pf 210 17 and and CC cord-349042-u9svz7pf 210 18 protein protein NN cord-349042-u9svz7pf 210 19 analysis analysis NN cord-349042-u9svz7pf 210 20 ) ) -RRB- cord-349042-u9svz7pf 210 21 , , , cord-349042-u9svz7pf 210 22 but but CC cord-349042-u9svz7pf 210 23 adding add VBG cord-349042-u9svz7pf 210 24 further further JJ cord-349042-u9svz7pf 210 25 levels level NNS cord-349042-u9svz7pf 210 26 of of IN cord-349042-u9svz7pf 210 27 analysis analysis NN cord-349042-u9svz7pf 210 28 Deep deep JJ cord-349042-u9svz7pf 210 29 sequencing sequencing NN cord-349042-u9svz7pf 210 30 reveals reveal VBZ cord-349042-u9svz7pf 210 31 cell cell NN cord-349042-u9svz7pf 210 32 - - HYPH cord-349042-u9svz7pf 210 33 type type NN cord-349042-u9svz7pf 210 34 - - HYPH cord-349042-u9svz7pf 210 35 specific specific JJ cord-349042-u9svz7pf 210 36 patterns pattern NNS cord-349042-u9svz7pf 210 37 of of IN cord-349042-u9svz7pf 210 38 single single JJ cord-349042-u9svz7pf 210 39 - - HYPH cord-349042-u9svz7pf 210 40 cell cell NN cord-349042-u9svz7pf 210 41 transcriptome transcriptome NN cord-349042-u9svz7pf 210 42 variation variation NN cord-349042-u9svz7pf 211 1 Amplified Amplified NNP cord-349042-u9svz7pf 211 2 RNA RNA NNP cord-349042-u9svz7pf 211 3 synthesized synthesize VBN cord-349042-u9svz7pf 211 4 from from IN cord-349042-u9svz7pf 211 5 limited limited JJ cord-349042-u9svz7pf 211 6 quantities quantity NNS cord-349042-u9svz7pf 211 7 of of IN cord-349042-u9svz7pf 211 8 heterogeneous heterogeneous JJ cord-349042-u9svz7pf 211 9 cDNA cdna NN cord-349042-u9svz7pf 211 10 Analysis Analysis NNP cord-349042-u9svz7pf 211 11 of of IN cord-349042-u9svz7pf 211 12 gene gene NN cord-349042-u9svz7pf 211 13 expression expression NN cord-349042-u9svz7pf 211 14 in in IN cord-349042-u9svz7pf 211 15 single single JJ cord-349042-u9svz7pf 211 16 live live JJ cord-349042-u9svz7pf 211 17 neurons neuron NNS cord-349042-u9svz7pf 212 1 Molecular molecular JJ cord-349042-u9svz7pf 212 2 profiles profile NNS cord-349042-u9svz7pf 212 3 of of IN cord-349042-u9svz7pf 212 4 pyramidal pyramidal JJ cord-349042-u9svz7pf 212 5 neurons neuron NNS cord-349042-u9svz7pf 212 6 in in IN cord-349042-u9svz7pf 212 7 the the DT cord-349042-u9svz7pf 212 8 superior superior JJ cord-349042-u9svz7pf 212 9 temporal temporal JJ cord-349042-u9svz7pf 212 10 cortex cortex NN cord-349042-u9svz7pf 212 11 in in IN cord-349042-u9svz7pf 212 12 schizophrenia schizophrenia NN cord-349042-u9svz7pf 212 13 Altered alter VBD cord-349042-u9svz7pf 212 14 expression expression NN cord-349042-u9svz7pf 212 15 of of IN cord-349042-u9svz7pf 212 16 glutamate glutamate NN cord-349042-u9svz7pf 212 17 signaling signaling NN cord-349042-u9svz7pf 212 18 , , , cord-349042-u9svz7pf 212 19 growth growth NN cord-349042-u9svz7pf 212 20 factor factor NN cord-349042-u9svz7pf 212 21 , , , cord-349042-u9svz7pf 212 22 and and CC cord-349042-u9svz7pf 212 23 glia glia NN cord-349042-u9svz7pf 212 24 genes gene NNS cord-349042-u9svz7pf 212 25 in in IN cord-349042-u9svz7pf 212 26 the the DT cord-349042-u9svz7pf 212 27 locus locus NN cord-349042-u9svz7pf 212 28 coeruleus coeruleu VBZ cord-349042-u9svz7pf 212 29 of of IN cord-349042-u9svz7pf 212 30 patients patient NNS cord-349042-u9svz7pf 212 31 with with IN cord-349042-u9svz7pf 212 32 major major JJ cord-349042-u9svz7pf 212 33 depression depression NN cord-349042-u9svz7pf 212 34 Effects effect NNS cord-349042-u9svz7pf 212 35 of of IN cord-349042-u9svz7pf 212 36 gender gender NN cord-349042-u9svz7pf 212 37 on on IN cord-349042-u9svz7pf 212 38 nigral nigral JJ cord-349042-u9svz7pf 212 39 gene gene NN cord-349042-u9svz7pf 212 40 expression expression NN cord-349042-u9svz7pf 212 41 and and CC cord-349042-u9svz7pf 212 42 parkinson parkinson NNP cord-349042-u9svz7pf 212 43 disease disease NN cord-349042-u9svz7pf 212 44 Predominance Predominance NNP cord-349042-u9svz7pf 212 45 of of IN cord-349042-u9svz7pf 212 46 neuronal neuronal JJ cord-349042-u9svz7pf 212 47 mRNAs mrna NNS cord-349042-u9svz7pf 212 48 in in IN cord-349042-u9svz7pf 212 49 individual individual JJ cord-349042-u9svz7pf 212 50 Alzheimer Alzheimer NNP cord-349042-u9svz7pf 212 51 's 's POS cord-349042-u9svz7pf 212 52 disease disease NN cord-349042-u9svz7pf 212 53 senile senile JJ cord-349042-u9svz7pf 212 54 plaques plaque NNS cord-349042-u9svz7pf 213 1 Gene gene NN cord-349042-u9svz7pf 213 2 expression expression NN cord-349042-u9svz7pf 213 3 profiles profile NNS cord-349042-u9svz7pf 213 4 of of IN cord-349042-u9svz7pf 213 5 human human JJ cord-349042-u9svz7pf 213 6 breast breast NN cord-349042-u9svz7pf 213 7 cancer cancer NN cord-349042-u9svz7pf 213 8 progression progression NN cord-349042-u9svz7pf 213 9 Expression expression NN cord-349042-u9svz7pf 213 10 profiling profiling NN cord-349042-u9svz7pf 213 11 of of IN cord-349042-u9svz7pf 213 12 ductal ductal JJ cord-349042-u9svz7pf 213 13 carcinoma carcinoma NN cord-349042-u9svz7pf 213 14 in in IN cord-349042-u9svz7pf 213 15 situ situ NN cord-349042-u9svz7pf 213 16 by by IN cord-349042-u9svz7pf 213 17 laser laser NN cord-349042-u9svz7pf 213 18 capture capture NN cord-349042-u9svz7pf 213 19 microdissection microdissection NN cord-349042-u9svz7pf 213 20 and and CC cord-349042-u9svz7pf 213 21 high high JJ cord-349042-u9svz7pf 213 22 - - HYPH cord-349042-u9svz7pf 213 23 density density NN cord-349042-u9svz7pf 214 1 oligonucleotide oligonucleotide NNP cord-349042-u9svz7pf 214 2 arrays arrays NNP cord-349042-u9svz7pf 214 3 Genome Genome NNP cord-349042-u9svz7pf 214 4 - - HYPH cord-349042-u9svz7pf 214 5 wide wide JJ cord-349042-u9svz7pf 214 6 screening screening NN cord-349042-u9svz7pf 214 7 of of IN cord-349042-u9svz7pf 214 8 genes gene NNS cord-349042-u9svz7pf 214 9 showing show VBG cord-349042-u9svz7pf 214 10 altered altered JJ cord-349042-u9svz7pf 214 11 expression expression NN cord-349042-u9svz7pf 214 12 in in IN cord-349042-u9svz7pf 214 13 liver liver NN cord-349042-u9svz7pf 214 14 metastases metastasis NNS cord-349042-u9svz7pf 214 15 of of IN cord-349042-u9svz7pf 214 16 human human JJ cord-349042-u9svz7pf 214 17 colorectal colorectal JJ cord-349042-u9svz7pf 214 18 cancers cancer NNS cord-349042-u9svz7pf 214 19 by by IN cord-349042-u9svz7pf 214 20 cDNA cdna NN cord-349042-u9svz7pf 214 21 microarray microarray NN cord-349042-u9svz7pf 214 22 Hepsin Hepsin NNP cord-349042-u9svz7pf 214 23 and and CC cord-349042-u9svz7pf 214 24 maspin maspin NNP cord-349042-u9svz7pf 214 25 are be VBP cord-349042-u9svz7pf 214 26 inversely inversely RB cord-349042-u9svz7pf 214 27 expressed express VBN cord-349042-u9svz7pf 214 28 in in IN cord-349042-u9svz7pf 214 29 laser laser NN cord-349042-u9svz7pf 214 30 capture capture NN cord-349042-u9svz7pf 214 31 microdissectioned microdissectione VBN cord-349042-u9svz7pf 214 32 prostate prostate NN cord-349042-u9svz7pf 214 33 cancer cancer NN cord-349042-u9svz7pf 214 34 Molecular Molecular NNP cord-349042-u9svz7pf 214 35 profiling profiling NN cord-349042-u9svz7pf 214 36 identifies identify VBZ cord-349042-u9svz7pf 214 37 prognostic prognostic JJ cord-349042-u9svz7pf 214 38 subgroups subgroup NNS cord-349042-u9svz7pf 214 39 of of IN cord-349042-u9svz7pf 214 40 pediatric pediatric JJ cord-349042-u9svz7pf 214 41 glioblastoma glioblastoma NNS cord-349042-u9svz7pf 214 42 and and CC cord-349042-u9svz7pf 214 43 shows show NNS cord-349042-u9svz7pf 214 44 increased increase VBN cord-349042-u9svz7pf 214 45 YB-1 YB-1 NNP cord-349042-u9svz7pf 214 46 expression expression NN cord-349042-u9svz7pf 214 47 in in IN cord-349042-u9svz7pf 214 48 tumors tumor NNS cord-349042-u9svz7pf 214 49 Gene gene NN cord-349042-u9svz7pf 214 50 expression expression NN cord-349042-u9svz7pf 214 51 profile profile NN cord-349042-u9svz7pf 214 52 of of IN cord-349042-u9svz7pf 214 53 glioblastoma glioblastoma NN cord-349042-u9svz7pf 214 54 multiforme multiforme NN cord-349042-u9svz7pf 214 55 invasive invasive JJ cord-349042-u9svz7pf 214 56 phenotype phenotype NN cord-349042-u9svz7pf 214 57 points point NNS cord-349042-u9svz7pf 214 58 to to IN cord-349042-u9svz7pf 214 59 new new JJ cord-349042-u9svz7pf 214 60 therapeutic therapeutic JJ cord-349042-u9svz7pf 214 61 targets target NNS cord-349042-u9svz7pf 214 62 Role role NN cord-349042-u9svz7pf 214 63 of of IN cord-349042-u9svz7pf 214 64 pancreatic pancreatic JJ cord-349042-u9svz7pf 214 65 cancer cancer NN cord-349042-u9svz7pf 214 66 - - HYPH cord-349042-u9svz7pf 214 67 derived derive VBN cord-349042-u9svz7pf 214 68 exosomes exosome NNS cord-349042-u9svz7pf 214 69 in in IN cord-349042-u9svz7pf 214 70 salivary salivary NN cord-349042-u9svz7pf 214 71 biomarker biomarker NN cord-349042-u9svz7pf 214 72 development development NN cord-349042-u9svz7pf 214 73 Transcription transcription NN cord-349042-u9svz7pf 214 74 - - HYPH cord-349042-u9svz7pf 214 75 based base VBN cord-349042-u9svz7pf 214 76 amplification amplification NN cord-349042-u9svz7pf 214 77 system system NN cord-349042-u9svz7pf 214 78 and and CC cord-349042-u9svz7pf 214 79 detection detection NN cord-349042-u9svz7pf 214 80 of of IN cord-349042-u9svz7pf 214 81 amplified amplify VBN cord-349042-u9svz7pf 214 82 human human JJ cord-349042-u9svz7pf 214 83 immunodeficiency immunodeficiency NN cord-349042-u9svz7pf 214 84 virus virus NN cord-349042-u9svz7pf 214 85 type type NN cord-349042-u9svz7pf 214 86 1 1 CD cord-349042-u9svz7pf 214 87 with with IN cord-349042-u9svz7pf 214 88 a a DT cord-349042-u9svz7pf 214 89 bead bead NN cord-349042-u9svz7pf 214 90 - - HYPH cord-349042-u9svz7pf 214 91 based base VBN cord-349042-u9svz7pf 214 92 sandwich sandwich NN cord-349042-u9svz7pf 214 93 hybridization hybridization NN cord-349042-u9svz7pf 214 94 format format NN cord-349042-u9svz7pf 214 95 Nucleic nucleic JJ cord-349042-u9svz7pf 214 96 acid acid NN cord-349042-u9svz7pf 214 97 sequence sequence NN cord-349042-u9svz7pf 214 98 - - HYPH cord-349042-u9svz7pf 214 99 based base VBN cord-349042-u9svz7pf 214 100 amplification amplification NN cord-349042-u9svz7pf 214 101 methods method NNS cord-349042-u9svz7pf 214 102 to to TO cord-349042-u9svz7pf 214 103 detect detect VB cord-349042-u9svz7pf 214 104 avian avian JJ cord-349042-u9svz7pf 214 105 influenza influenza NN cord-349042-u9svz7pf 214 106 virus virus NN cord-349042-u9svz7pf 214 107 Molecular molecular JJ cord-349042-u9svz7pf 214 108 signature signature NN cord-349042-u9svz7pf 214 109 of of IN cord-349042-u9svz7pf 214 110 clinical clinical JJ cord-349042-u9svz7pf 214 111 severity severity NN cord-349042-u9svz7pf 214 112 in in IN cord-349042-u9svz7pf 214 113 recovering recover VBG cord-349042-u9svz7pf 214 114 patients patient NNS cord-349042-u9svz7pf 214 115 with with IN cord-349042-u9svz7pf 214 116 severe severe JJ cord-349042-u9svz7pf 214 117 acute acute JJ cord-349042-u9svz7pf 214 118 respiratory respiratory JJ cord-349042-u9svz7pf 214 119 syndrome syndrome NN cord-349042-u9svz7pf 214 120 coronavirus coronavirus NN cord-349042-u9svz7pf 215 1 ( ( -LRB- cord-349042-u9svz7pf 215 2 SARS SARS NNP cord-349042-u9svz7pf 215 3 - - HYPH cord-349042-u9svz7pf 215 4 CoV CoV NNP cord-349042-u9svz7pf 215 5 ) ) -RRB- cord-349042-u9svz7pf 215 6 Identification Identification NNP cord-349042-u9svz7pf 215 7 of of IN cord-349042-u9svz7pf 215 8 RNA RNA NNP cord-349042-u9svz7pf 215 9 cargoes cargo NNS cord-349042-u9svz7pf 215 10 by by IN cord-349042-u9svz7pf 215 11 antibodypositioned antibodypositioned NNP cord-349042-u9svz7pf 215 12 RNA RNA NNP cord-349042-u9svz7pf 215 13 amplification amplification NN cord-349042-u9svz7pf 215 14 RNA RNA NNP cord-349042-u9svz7pf 215 15 cargoes cargo NNS cord-349042-u9svz7pf 215 16 associating associate VBG cord-349042-u9svz7pf 215 17 with with IN cord-349042-u9svz7pf 215 18 FMRP FMRP NNP cord-349042-u9svz7pf 215 19 reveal reveal VBP cord-349042-u9svz7pf 215 20 deficits deficit NNS cord-349042-u9svz7pf 215 21 in in IN cord-349042-u9svz7pf 215 22 cellular cellular JJ cord-349042-u9svz7pf 215 23 functioning functioning NN cord-349042-u9svz7pf 215 24 in in IN cord-349042-u9svz7pf 215 25 Fmr1 Fmr1 NNP cord-349042-u9svz7pf 215 26 null null JJ cord-349042-u9svz7pf 215 27 mice mouse NNS cord-349042-u9svz7pf 216 1 In in IN cord-349042-u9svz7pf 216 2 situ situ NN cord-349042-u9svz7pf 216 3 transcription transcription NN cord-349042-u9svz7pf 216 4 : : : cord-349042-u9svz7pf 216 5 specific specific JJ cord-349042-u9svz7pf 216 6 synthesis synthesis NN cord-349042-u9svz7pf 216 7 of of IN cord-349042-u9svz7pf 216 8 complementary complementary JJ cord-349042-u9svz7pf 216 9 DNA dna NN cord-349042-u9svz7pf 216 10 in in IN cord-349042-u9svz7pf 216 11 fixed fix VBN cord-349042-u9svz7pf 216 12 tissue tissue NN cord-349042-u9svz7pf 216 13 sections section NNS cord-349042-u9svz7pf 217 1 Protein protein NN cord-349042-u9svz7pf 217 2 quantification quantification NN cord-349042-u9svz7pf 217 3 from from IN cord-349042-u9svz7pf 217 4 complex complex JJ cord-349042-u9svz7pf 217 5 protein protein NN cord-349042-u9svz7pf 217 6 mixtures mixture NNS cord-349042-u9svz7pf 217 7 using use VBG cord-349042-u9svz7pf 217 8 a a DT cord-349042-u9svz7pf 217 9 proteomics proteomic NNS cord-349042-u9svz7pf 217 10 methodology methodology NN cord-349042-u9svz7pf 217 11 with with IN cord-349042-u9svz7pf 217 12 single single JJ cord-349042-u9svz7pf 217 13 - - HYPH cord-349042-u9svz7pf 217 14 cell cell NN cord-349042-u9svz7pf 217 15 resolution resolution NN cord-349042-u9svz7pf 217 16 A A NNP cord-349042-u9svz7pf 217 17 sensitive sensitive JJ cord-349042-u9svz7pf 217 18 and and CC cord-349042-u9svz7pf 217 19 highthroughput highthroughput NN cord-349042-u9svz7pf 217 20 assay assay NN cord-349042-u9svz7pf 217 21 to to TO cord-349042-u9svz7pf 217 22 detect detect VB cord-349042-u9svz7pf 217 23 low low JJ cord-349042-u9svz7pf 217 24 - - HYPH cord-349042-u9svz7pf 217 25 abundance abundance NN cord-349042-u9svz7pf 217 26 proteins protein NNS cord-349042-u9svz7pf 217 27 in in IN cord-349042-u9svz7pf 217 28 serum serum NN cord-349042-u9svz7pf 217 29 Improved improve VBN cord-349042-u9svz7pf 217 30 genome genome NN cord-349042-u9svz7pf 217 31 - - HYPH cord-349042-u9svz7pf 217 32 wide wide JJ cord-349042-u9svz7pf 217 33 localization localization NN cord-349042-u9svz7pf 217 34 by by IN cord-349042-u9svz7pf 217 35 ChIP chip NN cord-349042-u9svz7pf 217 36 - - HYPH cord-349042-u9svz7pf 217 37 chip chip NN cord-349042-u9svz7pf 217 38 using use VBG cord-349042-u9svz7pf 217 39 double double JJ cord-349042-u9svz7pf 217 40 - - HYPH cord-349042-u9svz7pf 217 41 round round NN cord-349042-u9svz7pf 217 42 T7 T7 NNP cord-349042-u9svz7pf 217 43 RNA RNA NNP cord-349042-u9svz7pf 217 44 polymerase polymerase NN cord-349042-u9svz7pf 217 45 - - HYPH cord-349042-u9svz7pf 217 46 based base VBN cord-349042-u9svz7pf 217 47 amplification amplification NN cord-349042-u9svz7pf 218 1 aRNA arna LS cord-349042-u9svz7pf 218 2 - - HYPH cord-349042-u9svz7pf 218 3 longSAGE longSAGE NNP cord-349042-u9svz7pf 218 4 : : : cord-349042-u9svz7pf 218 5 a a DT cord-349042-u9svz7pf 218 6 new new JJ cord-349042-u9svz7pf 218 7 approach approach NN cord-349042-u9svz7pf 218 8 to to TO cord-349042-u9svz7pf 218 9 generate generate VB cord-349042-u9svz7pf 218 10 SAGE SAGE NNP cord-349042-u9svz7pf 218 11 libraries library NNS cord-349042-u9svz7pf 218 12 from from IN cord-349042-u9svz7pf 218 13 microdissected microdissected JJ cord-349042-u9svz7pf 218 14 cells cell NNS cord-349042-u9svz7pf 218 15 Amplification Amplification NNP cord-349042-u9svz7pf 218 16 of of IN cord-349042-u9svz7pf 218 17 mRNA mrna NN cord-349042-u9svz7pf 218 18 populations population NNS cord-349042-u9svz7pf 218 19 using use VBG cord-349042-u9svz7pf 218 20 aRNA arna RB cord-349042-u9svz7pf 218 21 generated generate VBN cord-349042-u9svz7pf 218 22 from from IN cord-349042-u9svz7pf 218 23 immobilized immobilized JJ cord-349042-u9svz7pf 218 24 oligo(dT)-T7 oligo(dt)-t7 JJ cord-349042-u9svz7pf 218 25 primed prime VBN cord-349042-u9svz7pf 218 26 cDNA cDNA NNP cord-349042-u9svz7pf 218 27 A a DT cord-349042-u9svz7pf 218 28 robust robust JJ cord-349042-u9svz7pf 218 29 method method NN cord-349042-u9svz7pf 218 30 for for IN cord-349042-u9svz7pf 218 31 the the DT cord-349042-u9svz7pf 218 32 amplification amplification NN cord-349042-u9svz7pf 218 33 of of IN cord-349042-u9svz7pf 218 34 RNA RNA NNP cord-349042-u9svz7pf 218 35 in in IN cord-349042-u9svz7pf 218 36 the the DT cord-349042-u9svz7pf 218 37 sense sense NN cord-349042-u9svz7pf 219 1 orientation orientation NN cord-349042-u9svz7pf 220 1 An an DT cord-349042-u9svz7pf 220 2 automated automated JJ cord-349042-u9svz7pf 220 3 microwell microwell JJ cord-349042-u9svz7pf 220 4 platform platform NN cord-349042-u9svz7pf 220 5 for for IN cord-349042-u9svz7pf 220 6 large large JJ cord-349042-u9svz7pf 220 7 - - HYPH cord-349042-u9svz7pf 220 8 scale scale NN cord-349042-u9svz7pf 220 9 single single JJ cord-349042-u9svz7pf 220 10 cell cell NN cord-349042-u9svz7pf 220 11 RNA rna NN cord-349042-u9svz7pf 220 12 - - HYPH cord-349042-u9svz7pf 220 13 seq seq NNP cord-349042-u9svz7pf 220 14 Single Single NNP cord-349042-u9svz7pf 220 15 cell cell NN cord-349042-u9svz7pf 220 16 transcriptomics transcriptomic NNS cord-349042-u9svz7pf 220 17 of of IN cord-349042-u9svz7pf 220 18 hypothalamic hypothalamic JJ cord-349042-u9svz7pf 220 19 warm warm JJ cord-349042-u9svz7pf 220 20 sensitive sensitive JJ cord-349042-u9svz7pf 220 21 neurons neuron NNS cord-349042-u9svz7pf 220 22 that that WDT cord-349042-u9svz7pf 220 23 control control VBP cord-349042-u9svz7pf 220 24 core core NN cord-349042-u9svz7pf 220 25 body body NN cord-349042-u9svz7pf 220 26 temperature temperature NN cord-349042-u9svz7pf 220 27 and and CC cord-349042-u9svz7pf 220 28 fever fever NN cord-349042-u9svz7pf 220 29 response response NN cord-349042-u9svz7pf 220 30 : : : cord-349042-u9svz7pf 220 31 signaling signal VBG cord-349042-u9svz7pf 220 32 asymmetry asymmetry NN cord-349042-u9svz7pf 220 33 and and CC cord-349042-u9svz7pf 220 34 an an DT cord-349042-u9svz7pf 220 35 extension extension NN cord-349042-u9svz7pf 220 36 of of IN cord-349042-u9svz7pf 220 37 chemical chemical JJ cord-349042-u9svz7pf 220 38 neuroanatomy neuroanatomy NN cord-349042-u9svz7pf 221 1 Intron Intron NNP cord-349042-u9svz7pf 221 2 retention retention NN cord-349042-u9svz7pf 221 3 facilitates facilitate VBZ cord-349042-u9svz7pf 221 4 splice splice NN cord-349042-u9svz7pf 221 5 variant variant NN cord-349042-u9svz7pf 221 6 diversity diversity NN cord-349042-u9svz7pf 221 7 in in IN cord-349042-u9svz7pf 221 8 calciumactivated calciumactivated JJ cord-349042-u9svz7pf 221 9 big big JJ cord-349042-u9svz7pf 221 10 potassium potassium NN cord-349042-u9svz7pf 221 11 channel channel NN cord-349042-u9svz7pf 221 12 populations population NNS cord-349042-u9svz7pf 221 13 Analysis Analysis NNP cord-349042-u9svz7pf 221 14 of of IN cord-349042-u9svz7pf 221 15 mRNA mRNA NNP cord-349042-u9svz7pf 221 16 populations population NNS cord-349042-u9svz7pf 221 17 from from IN cord-349042-u9svz7pf 221 18 single single JJ cord-349042-u9svz7pf 221 19 live live NN cord-349042-u9svz7pf 221 20 and and CC cord-349042-u9svz7pf 221 21 fixed fix VBN cord-349042-u9svz7pf 221 22 cells cell NNS cord-349042-u9svz7pf 221 23 of of IN cord-349042-u9svz7pf 221 24 the the DT cord-349042-u9svz7pf 221 25 central central JJ cord-349042-u9svz7pf 221 26 nervous nervous JJ cord-349042-u9svz7pf 221 27 system system NN cord-349042-u9svz7pf 221 28 Primary primary JJ cord-349042-u9svz7pf 221 29 cell cell NN cord-349042-u9svz7pf 221 30 culture culture NN cord-349042-u9svz7pf 221 31 of of IN cord-349042-u9svz7pf 221 32 live live JJ cord-349042-u9svz7pf 221 33 neurosurgically neurosurgically RB cord-349042-u9svz7pf 221 34 resected resect VBN cord-349042-u9svz7pf 221 35 aged aged JJ cord-349042-u9svz7pf 221 36 adult adult NN cord-349042-u9svz7pf 221 37 human human JJ cord-349042-u9svz7pf 221 38 brain brain NN cord-349042-u9svz7pf 221 39 cells cell NNS cord-349042-u9svz7pf 221 40 and and CC cord-349042-u9svz7pf 222 1 single single JJ cord-349042-u9svz7pf 222 2 cell cell NN cord-349042-u9svz7pf 222 3 transcriptomics transcriptomic NNS cord-349042-u9svz7pf 222 4 Serotonergic Serotonergic NNP cord-349042-u9svz7pf 222 5 neuron neuron NN cord-349042-u9svz7pf 222 6 regulation regulation NN cord-349042-u9svz7pf 222 7 informed inform VBN cord-349042-u9svz7pf 222 8 by by IN cord-349042-u9svz7pf 222 9 in in IN cord-349042-u9svz7pf 222 10 vivo vivo FW cord-349042-u9svz7pf 222 11 single single JJ cord-349042-u9svz7pf 222 12 - - HYPH cord-349042-u9svz7pf 222 13 cell cell NN cord-349042-u9svz7pf 222 14 transcriptomics transcriptomic NNS cord-349042-u9svz7pf 222 15 CEL CEL NNP cord-349042-u9svz7pf 222 16 - - HYPH cord-349042-u9svz7pf 222 17 seq seq NNP cord-349042-u9svz7pf 222 18 : : : cord-349042-u9svz7pf 222 19 single single JJ cord-349042-u9svz7pf 222 20 - - HYPH cord-349042-u9svz7pf 222 21 cell cell NN cord-349042-u9svz7pf 222 22 RNA rna NN cord-349042-u9svz7pf 222 23 - - HYPH cord-349042-u9svz7pf 222 24 seq seq NN cord-349042-u9svz7pf 222 25 by by IN cord-349042-u9svz7pf 222 26 multiplexed multiplexed JJ cord-349042-u9svz7pf 222 27 linear linear JJ cord-349042-u9svz7pf 222 28 amplification amplification NN cord-349042-u9svz7pf 223 1 Assessing assess VBG cord-349042-u9svz7pf 223 2 characteristics characteristic NNS cord-349042-u9svz7pf 223 3 of of IN cord-349042-u9svz7pf 223 4 RNA rna NN cord-349042-u9svz7pf 223 5 amplification amplification NN cord-349042-u9svz7pf 223 6 methods method NNS cord-349042-u9svz7pf 223 7 for for IN cord-349042-u9svz7pf 223 8 single single JJ cord-349042-u9svz7pf 223 9 cell cell NN cord-349042-u9svz7pf 223 10 RNA rna NN cord-349042-u9svz7pf 223 11 sequencing sequence VBG cord-349042-u9svz7pf 223 12 Single single JJ cord-349042-u9svz7pf 223 13 - - HYPH cord-349042-u9svz7pf 223 14 cell cell NN cord-349042-u9svz7pf 223 15 whole whole JJ cord-349042-u9svz7pf 223 16 - - HYPH cord-349042-u9svz7pf 223 17 genome genome NN cord-349042-u9svz7pf 223 18 analyses analysis NNS cord-349042-u9svz7pf 223 19 by by IN cord-349042-u9svz7pf 223 20 linear linear JJ cord-349042-u9svz7pf 223 21 amplification amplification NN cord-349042-u9svz7pf 223 22 via via IN cord-349042-u9svz7pf 223 23 transposon transposon NN cord-349042-u9svz7pf 223 24 insertion insertion NN cord-349042-u9svz7pf 224 1 ( ( -LRB- cord-349042-u9svz7pf 224 2 LIANTI LIANTI NNP cord-349042-u9svz7pf 224 3 ) ) -RRB- cord-349042-u9svz7pf 224 4 Expression expression NN cord-349042-u9svz7pf 224 5 monitoring monitoring NN cord-349042-u9svz7pf 224 6 by by IN cord-349042-u9svz7pf 224 7 hybridization hybridization NN cord-349042-u9svz7pf 224 8 to to IN cord-349042-u9svz7pf 224 9 high high JJ cord-349042-u9svz7pf 224 10 - - HYPH cord-349042-u9svz7pf 224 11 density density NN cord-349042-u9svz7pf 224 12 oligonucleotide oligonucleotide NN cord-349042-u9svz7pf 224 13 arrays array NNS cord-349042-u9svz7pf 225 1 Dopamine dopamine NN cord-349042-u9svz7pf 225 2 receptor receptor NN cord-349042-u9svz7pf 225 3 subtypes subtype VBZ cord-349042-u9svz7pf 225 4 colocalize colocalize NN cord-349042-u9svz7pf 225 5 in in IN cord-349042-u9svz7pf 225 6 rat rat NN cord-349042-u9svz7pf 225 7 striatonigral striatonigral JJ cord-349042-u9svz7pf 225 8 neurons neuron NNS cord-349042-u9svz7pf 225 9 Embryonic embryonic JJ cord-349042-u9svz7pf 225 10 neuronal neuronal JJ cord-349042-u9svz7pf 225 11 markers marker NNS cord-349042-u9svz7pf 225 12 in in IN cord-349042-u9svz7pf 225 13 tuberous tuberous JJ cord-349042-u9svz7pf 225 14 sclerosis sclerosis NN cord-349042-u9svz7pf 225 15 : : : cord-349042-u9svz7pf 225 16 single single JJ cord-349042-u9svz7pf 225 17 - - HYPH cord-349042-u9svz7pf 225 18 cell cell NN cord-349042-u9svz7pf 225 19 molecular molecular JJ cord-349042-u9svz7pf 225 20 pathology pathology NN cord-349042-u9svz7pf 225 21 Fidelity Fidelity NNP cord-349042-u9svz7pf 225 22 and and CC cord-349042-u9svz7pf 225 23 enhanced enhanced JJ cord-349042-u9svz7pf 225 24 sensitivity sensitivity NN cord-349042-u9svz7pf 225 25 of of IN cord-349042-u9svz7pf 225 26 differential differential JJ cord-349042-u9svz7pf 225 27 transcription transcription NN cord-349042-u9svz7pf 225 28 profiles profile NNS cord-349042-u9svz7pf 225 29 following follow VBG cord-349042-u9svz7pf 225 30 linear linear JJ cord-349042-u9svz7pf 225 31 amplification amplification NN cord-349042-u9svz7pf 225 32 of of IN cord-349042-u9svz7pf 225 33 nanogram nanogram NN cord-349042-u9svz7pf 225 34 amounts amount NNS cord-349042-u9svz7pf 225 35 of of IN cord-349042-u9svz7pf 225 36 endothelial endothelial JJ cord-349042-u9svz7pf 225 37 mRNA mRNA NNP cord-349042-u9svz7pf 225 38 Advantages Advantages NNPS cord-349042-u9svz7pf 225 39 of of IN cord-349042-u9svz7pf 225 40 mRNA mrna NN cord-349042-u9svz7pf 225 41 amplification amplification NN cord-349042-u9svz7pf 225 42 for for IN cord-349042-u9svz7pf 225 43 microarray microarray NN cord-349042-u9svz7pf 225 44 analysis analysis NN cord-349042-u9svz7pf 226 1 Massively massively RB cord-349042-u9svz7pf 226 2 parallel parallel JJ cord-349042-u9svz7pf 226 3 single single JJ cord-349042-u9svz7pf 226 4 - - HYPH cord-349042-u9svz7pf 226 5 cell cell NN cord-349042-u9svz7pf 226 6 RNA rna NN cord-349042-u9svz7pf 226 7 - - HYPH cord-349042-u9svz7pf 226 8 seq seq NN cord-349042-u9svz7pf 226 9 for for IN cord-349042-u9svz7pf 226 10 marker marker NN cord-349042-u9svz7pf 226 11 - - HYPH cord-349042-u9svz7pf 226 12 free free JJ cord-349042-u9svz7pf 226 13 decomposition decomposition NN cord-349042-u9svz7pf 226 14 of of IN cord-349042-u9svz7pf 226 15 tissues tissue NNS cord-349042-u9svz7pf 226 16 into into IN cord-349042-u9svz7pf 226 17 cell cell NN cord-349042-u9svz7pf 226 18 types type NNS cord-349042-u9svz7pf 226 19 On on IN cord-349042-u9svz7pf 226 20 the the DT cord-349042-u9svz7pf 226 21 nature nature NN cord-349042-u9svz7pf 226 22 and and CC cord-349042-u9svz7pf 226 23 differential differential JJ cord-349042-u9svz7pf 226 24 distribution distribution NN cord-349042-u9svz7pf 226 25 of of IN cord-349042-u9svz7pf 226 26 mRNAs mRNAs NNPS cord-349042-u9svz7pf 226 27 in in IN cord-349042-u9svz7pf 226 28 hippocampal hippocampal JJ cord-349042-u9svz7pf 226 29 neurites neurite NNS cord-349042-u9svz7pf 226 30 : : : cord-349042-u9svz7pf 226 31 implications implication NNS cord-349042-u9svz7pf 226 32 for for IN cord-349042-u9svz7pf 226 33 neuronal neuronal JJ cord-349042-u9svz7pf 226 34 functioning function VBG cord-349042-u9svz7pf 226 35 Molecular molecular JJ cord-349042-u9svz7pf 226 36 characterization characterization NN cord-349042-u9svz7pf 226 37 of of IN cord-349042-u9svz7pf 226 38 the the DT cord-349042-u9svz7pf 226 39 dendritic dendritic JJ cord-349042-u9svz7pf 226 40 growth growth NN cord-349042-u9svz7pf 226 41 cone cone NN cord-349042-u9svz7pf 226 42 : : : cord-349042-u9svz7pf 227 1 regulated regulate VBN cord-349042-u9svz7pf 227 2 mRNA mRNA NNP cord-349042-u9svz7pf 227 3 transport transport NN cord-349042-u9svz7pf 227 4 and and CC cord-349042-u9svz7pf 227 5 local local JJ cord-349042-u9svz7pf 227 6 protein protein NN cord-349042-u9svz7pf 227 7 synthesis synthesis NN cord-349042-u9svz7pf 227 8 Cytoplasmic Cytoplasmic NNP cord-349042-u9svz7pf 227 9 intron intron NN cord-349042-u9svz7pf 227 10 sequence sequence NN cord-349042-u9svz7pf 227 11 - - HYPH cord-349042-u9svz7pf 227 12 retaining retain VBG cord-349042-u9svz7pf 227 13 transcripts transcript NNS cord-349042-u9svz7pf 227 14 can can MD cord-349042-u9svz7pf 227 15 be be VB cord-349042-u9svz7pf 227 16 dendritically dendritically RB cord-349042-u9svz7pf 227 17 targeted target VBN cord-349042-u9svz7pf 227 18 via via IN cord-349042-u9svz7pf 227 19 ID ID NNP cord-349042-u9svz7pf 227 20 element element NN cord-349042-u9svz7pf 227 21 retrotransposons retrotransposon VBZ cord-349042-u9svz7pf 227 22 Highfidelity Highfidelity NNP cord-349042-u9svz7pf 227 23 mRNA mrna NN cord-349042-u9svz7pf 227 24 amplification amplification NN cord-349042-u9svz7pf 227 25 for for IN cord-349042-u9svz7pf 227 26 gene gene NN cord-349042-u9svz7pf 227 27 profiling profile VBG cord-349042-u9svz7pf 227 28 Prospective prospective JJ cord-349042-u9svz7pf 227 29 molecular molecular JJ cord-349042-u9svz7pf 227 30 profiling profiling NN cord-349042-u9svz7pf 227 31 of of IN cord-349042-u9svz7pf 227 32 melanoma melanoma NN cord-349042-u9svz7pf 227 33 metastases metastasis NNS cord-349042-u9svz7pf 227 34 suggests suggest VBZ cord-349042-u9svz7pf 227 35 classifiers classifier NNS cord-349042-u9svz7pf 227 36 of of IN cord-349042-u9svz7pf 227 37 immune immune JJ cord-349042-u9svz7pf 227 38 responsiveness responsiveness NN cord-349042-u9svz7pf 227 39 Human human JJ cord-349042-u9svz7pf 227 40 transcriptome transcriptome NN cord-349042-u9svz7pf 227 41 array array NN cord-349042-u9svz7pf 227 42 for for IN cord-349042-u9svz7pf 227 43 high high JJ cord-349042-u9svz7pf 227 44 - - HYPH cord-349042-u9svz7pf 227 45 throughput throughput NN cord-349042-u9svz7pf 227 46 clinical clinical JJ cord-349042-u9svz7pf 227 47 studies study NNS cord-349042-u9svz7pf 227 48 CEL cel NN cord-349042-u9svz7pf 227 49 - - HYPH cord-349042-u9svz7pf 227 50 Seq2 Seq2 NNP cord-349042-u9svz7pf 227 51 : : : cord-349042-u9svz7pf 228 1 sensitive sensitive JJ cord-349042-u9svz7pf 228 2 highly highly RB cord-349042-u9svz7pf 228 3 - - HYPH cord-349042-u9svz7pf 228 4 multiplexed multiplexe VBN cord-349042-u9svz7pf 228 5 single single JJ cord-349042-u9svz7pf 228 6 - - HYPH cord-349042-u9svz7pf 228 7 cell cell NN cord-349042-u9svz7pf 228 8 RNA RNA NNP cord-349042-u9svz7pf 228 9 - - HYPH cord-349042-u9svz7pf 228 10 seq seq NNP cord-349042-u9svz7pf 228 11 Single single JJ cord-349042-u9svz7pf 228 12 - - HYPH cord-349042-u9svz7pf 228 13 cell cell NN cord-349042-u9svz7pf 228 14 barcoding barcoding NN cord-349042-u9svz7pf 228 15 and and CC cord-349042-u9svz7pf 228 16 sequencing sequencing NN cord-349042-u9svz7pf 228 17 using use VBG cord-349042-u9svz7pf 228 18 droplet droplet NN cord-349042-u9svz7pf 228 19 microfluidics microfluidic NNS cord-349042-u9svz7pf 228 20 Droplet Droplet NNP cord-349042-u9svz7pf 228 21 barcoding barcoding NN cord-349042-u9svz7pf 228 22 for for IN cord-349042-u9svz7pf 228 23 single single JJ cord-349042-u9svz7pf 228 24 - - HYPH cord-349042-u9svz7pf 228 25 cell cell NN cord-349042-u9svz7pf 228 26 transcriptomics transcriptomic NNS cord-349042-u9svz7pf 228 27 applied apply VBD cord-349042-u9svz7pf 228 28 to to IN cord-349042-u9svz7pf 228 29 embryonic embryonic JJ cord-349042-u9svz7pf 228 30 stem stem NN cord-349042-u9svz7pf 228 31 cells cell NNS cord-349042-u9svz7pf 228 32 Amplified amplify VBD cord-349042-u9svz7pf 228 33 expression expression NN cord-349042-u9svz7pf 228 34 profiling profiling NN cord-349042-u9svz7pf 228 35 of of IN cord-349042-u9svz7pf 228 36 platelet platelet NN cord-349042-u9svz7pf 228 37 transcriptome transcriptome NN cord-349042-u9svz7pf 228 38 reveals reveal VBZ cord-349042-u9svz7pf 228 39 changes change NNS cord-349042-u9svz7pf 228 40 in in IN cord-349042-u9svz7pf 228 41 arginine arginine NN cord-349042-u9svz7pf 228 42 metabolic metabolic JJ cord-349042-u9svz7pf 228 43 pathways pathway NNS cord-349042-u9svz7pf 228 44 in in IN cord-349042-u9svz7pf 228 45 patients patient NNS cord-349042-u9svz7pf 228 46 with with IN cord-349042-u9svz7pf 228 47 sickle sickle NN cord-349042-u9svz7pf 228 48 cell cell NN cord-349042-u9svz7pf 228 49 disease disease NN