id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_2ntdu5t7rve6vkexrvobwuslhe	Tamara Maiuri	Dysregulation of poly ADP ribose signaling in HD and its potential as a therapeutic target	2019	1	.pdf	application/pdf	1163	149	63	HUNTINGTIN BINDS PARP ELEVATED PAR IN HD HUNTINGTIN BINDS POLY ADP RIBOSE Figure 1: Huntingtin interacts with PARylated proteins Huntingtin was immunoprecipitated with EPR5526 and associated proteins separated by SDS-PAGE and immunoblotted with the indicated • Huntingtin acts as a scaffold for DNA repair proteins (Maiuri et al, 2017) • Poly ADP ribose (PAR) is a post-translational modi�cation generated Figure 4: Huntingtin interacts with Poly ADP ribose polymerase was immunoprecipitated with EPR5526 (Abcam) and associated proteins separated by SDS-PAGE and immunoblotted with anti-PARP (BD Biosciences) and anti-huntingtin (MAB2166, Millipore). B. Puri�ed huntingtin (Harding et al, 2019) and PARP (Trevigen) were incubated in the presence of activated DNA and NAD+. • Levels of DNA damage are elevated in HD cells and tissues (Maiuri et al, 2017; Askeland et al, 2018; Castaldo et al, 2019) dysfunction in peripheral blood mononuclear cells from Huntington's disease patients. Poly (ADP-ribose) binds to specific domains in DNA damage 	./cache/work_2ntdu5t7rve6vkexrvobwuslhe.pdf	./txt/work_2ntdu5t7rve6vkexrvobwuslhe.txt