id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-011012-5mev3otu	Rathore, Abhishek Singh	Production and immunogenicity of Fubc subunit protein redesigned from DENV envelope protein	2020-03-30		.txt	text/plain	6729	316	52	It was observed by ELISA that Fubc fusion protein elicited higher serum IgG antibody response either in the presence or in absence of Freund's adjuvant in comparison to the immune response of Fu and bc peptides separately. Herein, we have expressed the recombinant Fubc antigenic protein, optimised its large-scale purification protocol and finally evaluated its protein-specific immune response in BALB/c mice. Therefore, these two conserved Fu and bc loops have been selected to design a recombinant Fubc antigenic protein for the development of dengue subunit vaccine. Immune response to the purified Fubc protein in BALB/c mice From indirect ELISA, it was observed that the serum IgG levels in both male and female groups treated with Fubc proteins were significantly higher than those treated with only adjuvant and PBS control (Fig. 4a, b) . Antibodies to envelope glycoprotein of dengue virus during the natural course of infection are predominantly cross-reactive and recognize epitopes containing highly conserved residues at the fusion loop of domain II	./cache/cord-011012-5mev3otu.txt	./txt/cord-011012-5mev3otu.txt