id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-012064-egzl6zk9	Koopman, Frank W.	C(1) compounds as auxiliary substrate for engineered Pseudomonas putida S12	2009-06-01		.txt	text/plain	5333	303	45	The hps and phi genes of Bacillus brevis, encoding two key steps of the ribulose monophosphate (RuMP) pathway, were introduced to construct a pathway for the metabolism of the toxic methanol oxidation intermediate formaldehyde. Black arrows indicate the assimilatory RuMP pathway, dashed arrows indicate the dissimilatory RuMP pathway, gray arrows indicate the linear oxidation of formaldehyde to carbon dioxide formate dehydrogenase (Fmd) from the methylotrophic yeast Hansenula polymorpha in Saccharomyces cerevisiae resulted in an increased biomass yield with formaldehyde as auxiliary substrate (Baerends et al. The RuMP pathway strain showed significantly improved performance over the control strain, achieving a biomass yield-on-glucose of 91% when using formaldehyde as auxiliary substrate. putida S12pJNNhp(t) and an empty vector control strain were cultured in a C-limited chemostat with glucose as the primary carbon source and formaldehyde as auxiliary substrate. b Biomass yield increase (on glucose) as function of relative formaldehyde concentration in chemostat cultures of strains S12pJNNhp(t) (triangles) and S12pJNN (t) (squares).	./cache/cord-012064-egzl6zk9.txt	./txt/cord-012064-egzl6zk9.txt