id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-012086-sqv56mmq	Meijnen, Jean-Paul	Improved p-hydroxybenzoate production by engineered Pseudomonas putida S12 by using a mixed-substrate feeding strategy	2011-02-02		.txt	text/plain	4439	252	49	The key precursors for p-hydroxybenzoate production by engineered Pseudomonas putida S12 are phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P), for which the pentose phosphate (PP) pathway is an important source. Even without xylose-co-feeding, p-hydroxybenzoate production was improved in the evolved xylose-utilizing strain, which may indicate an intrinsically elevated PP pathway activity. Pseudomonas putida S12 is a solvent-tolerant bacterium that has been developed as a platform host for the production of a range of substituted aromatic compounds such as phenol, tcinnamate, p-coumarate, p-hydroxybenzoate, and p-hydroxystyrene (Nijkamp et al. putida S12pal_xylB7 on glucose as single carbon source showed a product-tosubstrate yield of 4.9 Cmol%, with a specific production (Fig. 3b) . Because of the improved biomass yield, the effect of the primary substrate on the specific phydroxybenzoate production rate q p was limited: the q p on glycerol (8.57 μmol C (g CDW) −1 h −1 ) was only slightly higher than on glucose (7.96 μmol C (g CDW) −1 h −1 ).	./cache/cord-012086-sqv56mmq.txt	./txt/cord-012086-sqv56mmq.txt