id author title date pages extension mime words sentences flesch summary cache txt cord-004681-02wem2u3 Wada, R. Ultrastructure and immuno-cytochemistry of BHK-21 cells infected with a modified Bucyrus strain of equine arteritis virus 1995 .txt text/plain 2044 123 55 title: Ultrastructure and immuno-cytochemistry of BHK-21 cells infected with a modified Bucyrus strain of equine arteritis virus Morphogenesis of a modified Bucyrus strain of equine arteritis virus (EAV) in BHK-21 cells was studied. Bacillary tubules were first detected in the cytoplasm 8 h after infection, and mature virions 79 to 122 nm in diameter, 101 nm on average, were mostly observed in the cisternae of the rough endoplasmic reticulum (RER) at 12 h or later. Equine arteritis virus (EAV) has been classified into the Togaviridae family [22] on the basis of the virion size and morphology [23] . In the present study a mutant virus showing higher growth on BHK-21 cells than the parental Bucyrus strain of EAV [11] was examined for the fine structure, its morphogenesis and intracellular localization of viral antigen. Through cross-sections of the outer layer of the virion, a grape-like structure seemed to be subunits 13 to 20 nm in diameter around the core (Fig. 3c) . ./cache/cord-004681-02wem2u3.txt ./txt/cord-004681-02wem2u3.txt