id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004720-r6b34tjm	Kaiser, C. J.	Inhibition by monensin of human cytomegalovirus DNA replication	1987		.txt	text/plain	4357	246	50	Phosphonoacetic acid (PAA) and monensin were purchased from Sigma, Deisenhofen, Federal Republic of Germany, tunieamycin from Calbiochem, La Jolla, U.S.A. In a typicM experiment for the determination of infected cell DNA synthesis in the presence of monensin, parallel cultures of HFF (5 × 106 cells) were infected by HCMV (MOI of 1) and pulse labelled with att-thymidine (5 ~Ci/ml) during the late phase of the consecutive infectious cycle (28) . To examine whether pretreated cells support, viral replication, confluent serum-starved HFF were exposed to various concentrations of monensin prior to virus infection and subsequently pulse labelled with 3H-thymidine during the interval of expected viral DNA synthesis (28; Fig. 2 A) . This protocol again did not prevent subsequent recovery of viral DNA replication within 48-72 hours after removal, an observation which was based on the comparative anMysis by isopycnie centrifugation in CsC1 of DNA samples from untreated and drug-treated infected cultures labelled during corresponding pulse intervals (Fig. 2 B, w/o and monint) .	./cache/cord-004720-r6b34tjm.txt	./txt/cord-004720-r6b34tjm.txt