id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004769-hhge62sl	Remond, M.	Partial DNA cloning and sequencing of a canine parvovirus vaccine strain: application of nucleic acid hybridization to the diagnosis of canine parvovirus disease	1992		.txt	text/plain	3270	221	62	title: Partial DNA cloning and sequencing of a canine parvovirus vaccine strain: application of nucleic acid hybridization to the diagnosis of canine parvovirus disease The cloning and sequencing of anEco RI-Pst I fragment derived from the replicative form of a canine parvovirus (CPV) vaccine strain are reported. In order to improve CPV diagnosis, radioactively labelled RNA or DNA and biotin labelled DNA obtained by random priming of the recombinant plasmid were used as probes mainly on gut or stool samples from naturally infected dogs. Results of filter hybridization correlated well with histopathological diagnosis of parvovirus infection and with hemagglutination tests performed on dog faeces. To elucidate whether the deletion observed was originally in the vaccine or was generated by cell culture passages in our laboratory, DNA sequence was determined on CPV-b 108 after PCR amplification of a 1000 bp fragment including the deleted region and the two H i n d I I I restriction sites (Fig. 1) .	./cache/cord-004769-hhge62sl.txt	./txt/cord-004769-hhge62sl.txt