id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004793-6yh36r0w	Choi, C. S.	Replication of two porcine parvovirus isolates at non-permissive temperatures	1990		.txt	text/plain	3234	178	57	Previous studies have shown that replication in vitro of the porcine parvovirus (PPV) isolate, KBSH, was restricted at 39°C but not at 37°C. In this study, Kresse and KBSH isolates were passaged up to ten times in swine testicle (ST) cells at non-permissive temperatures, and at subsequent passage viral protein synthesis, viral DNA synthesis, and progeny virus were evaluated. Kresse and KBSH isolates were serially passaged at non-permissive temperatures in an attempt to evaluate the effect of in vitro passages on the pattern of virus replication. KBSH appeared to gain the ability to replicate in swine fetuses following serial cell culture passages at 39 °C evidenced by fetal death and virus detection in fetal tissues. Furthermore, KBSH isolate passaged at 39 °C for 10 times now showed the ability to replicate in swine fetuses, indicating that pathogenicity can be recovered by serial adaptation at the mean body temperature of pigs, 39 °C.	./cache/cord-004793-6yh36r0w.txt	./txt/cord-004793-6yh36r0w.txt