id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004798-5budstbg	Takayama, N.	An improved method for titration of mouse hepatitis virus type 3 in a mouse cell culture	1976		.txt	text/plain	924	56	73	Plaque assay in DBT cells with DEAE-dextran and trypsin presents a titration system for MHV3 as sensitive as the LD(50) assay in mice. In DBT cells overlaid with ME)/I containing 1 per cent Noble agar, 5 per cent CS, 10 per cent TPB, and 1 : 10,000 neutral red, MHV3-plaques of 0.6--1.4 mm (mean value = 0.9 ram) in diameter could be counted at 40 hours p.i. The plaques were clearly-defined and sometimes contained a deeply-stained area at their center. To ira.prove the sensitivity of the plaque assay we examined the effect of diethylaminoethyl-dextran (DEAE-D) on the plaque formation and plaque diameter of MHV3, as BRADBUllNE and TYRREL]5 had reported that DEAE-D added to overlay agar increased the plaque number of human coronavirus (2). 1%eplication and plaque formation of mouse hepatitis virus (MttV-2) in mouse cell line DBT culture	./cache/cord-004798-5budstbg.txt	./txt/cord-004798-5budstbg.txt