id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004802-rhkrmftn	Hoshino, Y.	Isolation and characterization of a canine rotavirus	1982		.txt	text/plain	2902	172	47	Replication of the virus in cell culture was found to be less dependent upon trypsin than that of human, bovine and porcine rotaviruses. Reproducible, sharp-edged plaques of various sizes were produced by the canine rotavirus in an established cell line of fetal rhesus monkey kidney, MA104, under overlays of carboxymethyl cellulose or agarose. By plaque reduction neutralization assay, a two-way antigenic relationship was found between the canine (CU-1) and simian (rhesus MMU 18006 and SA-11) rotaviruses. Although canine rotavirus readily replicated and produced CPE in monolayer cultures of MA104 cells in the absence of trypsin, detectable plaques were not formed even 8 D P I under the overlays of CMC or agarose without trypsin. Since both IFT and CFT detect only group-specific antibodies, and rotaviruses from one species can infect another species (5, 8, 34, 36, 46, 50, 51, 52, 55) , it was not clear from these studies whether the antibodies in the dogs resulted from infection with distinctly canine rotavirus or with human or some other rotavirus.	./cache/cord-004802-rhkrmftn.txt	./txt/cord-004802-rhkrmftn.txt