id author title date pages extension mime words sentences flesch summary cache txt cord-004808-6w9n03fy Sekiguchi, K. Detection of equine arteritis virus (EAV) by polymerase chain reaction (PCR) and differentiation of EAV strains by restriction enzyme analysis of PCR products 1995 .txt text/plain 2056 116 59 title: Detection of equine arteritis virus (EAV) by polymerase chain reaction (PCR) and differentiation of EAV strains by restriction enzyme analysis of PCR products A polymerase chain reaction (PCR) based assay capable of detecting and differentiating seven strains of equine arteritis virus (EAV) from around the world was developed. A potymerase chain reaction (PCR) based assay capable of detecting and differentiating seven strains of equine arteritis virus (EAV) from around the world was developed. Viral RNA from cell culture fluids infected with each of the seven EAV strains and RNA from the live vaccine, Arvac, was detected by PCR using four sets of primers. The objective of this study was to detect each of the EAV strains using PCR, and to differentiate the strains through restriction enzyme analysis of their PCR products, based on the M protein nucleotide sequence data of the strains, together with those of the Bucyrus [7] and modified Bucyrus strain [251. ./cache/cord-004808-6w9n03fy.txt ./txt/cord-004808-6w9n03fy.txt