id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279676-sk9xyd1r	Carossino, Mariano	Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques	2020-08-05		.txt	text/plain	1643	94	45	In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. In situ hybridization (ISH) and immunohistochemistry (IHC) techniques allow visualization of viral nucleic acid and protein antigens, respectively, within tissues and cells. For this purpose, the development of SARS-CoV-2-specific ISH and IHC are both critical for the assessment of viral distribution, cell tropism, and cytopathology within tissues, complementing classical histopathology, various molecular tools, and serological assays. Thus, the objective of this study was to develop RNAscope ® ISH and IHC methods for the detection of SARS-CoV-2-specific antigen and RNA in infected cells that can be utilized for both research (e.g., studies involving experimentally and naturally infected animals) and diagnostic purposes. Furthermore, the development of IHC and ISH tools is of utmost significance for understanding the pathogenesis of SARS-CoV-2 by characterizing the viral tissue distribution/cellular tropism in animal models and humans.	./cache/cord-279676-sk9xyd1r.txt	./txt/cord-279676-sk9xyd1r.txt