id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-281410-y558a5jf	Akashi, H.	Propagation of the Kakegawa strain of bovine coronavirus in suckling mice, rats and hamsters	1981		.txt	text/plain	1120	85	63	title: Propagation of the Kakegawa strain of bovine coronavirus in suckling mice, rats and hamsters Infected animals died with nervous symptoms, and serial passage was readily accomplished by intracerebral inoculation with brain emulsions. Infected animals died with nervous symptoms, and serial passage was readily accomplished by intraeerebral inoculation with brain emulsions. The present paper describes briefly our recent observation that the Kakegawa strain of BCV readily propagates in suckling mice, rats and hamsters. Four-day-old mouse 3 days after intraeerebral inoculation with the 3rd mouse brain passaged Kakegawa strain (left) and an uninoeulated 4-day-old mouse (right) Four litters (10 to 12/litter) of suckling mice were inoculated intracerebrally with infected tissue culture fluid containing 106-5 TCID50/ml. These findings showed that the viruses recovered from the brains of affected animals were antigenieally the same as the cell culture passaged virus and could be clearly differentiated from the MttV strain 2.	./cache/cord-281410-y558a5jf.txt	./txt/cord-281410-y558a5jf.txt