id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-283525-kvcqayl4	Wei, Zhan-Yong	Nitric oxide inhibits the replication cycle of porcine parvovirus in vitro	2009-05-13		.txt	text/plain	2366	124	51	This study investigated the inhibitory effect and mechanism of nitric oxide (NO) on porcine parvovirus (PPV) replication in PK-15 cells. The results showed that two NO-generating compounds, S-nitroso-l-acetylpenicillamine (SNAP) and l-arginine (LA), at a noncytotoxic concentration could reduce PPV replication in a dose-dependent manner and that this anti-PPV effect could be reversed by the NO synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (l-NAME). As shown in Fig. 1a , SNAP and LA have the ability to inhibit PPV replication in PK-15 cells, and there is a dose-dependent relationship between the inhibitory effect and the SNAP (or LA) concentration. As shown in Fig. 2 , the PFU value increased with later addition of SNAP (or LA), and adding SNAP (or LA) 6 or 3 h prior to viral infection resulted in the highest level of inhibition. Our results demonstrate that NO specially inhibits the PPV replication cycle during the step of viral protein synthesis (Fig. 3b) .	./cache/cord-283525-kvcqayl4.txt	./txt/cord-283525-kvcqayl4.txt