id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-285892-tp6mlqtw	Li, Yingli	Isolation of two Chinese bovine enteroviruses and sequence analysis of their complete genomes	2012-08-01		.txt	text/plain	2956	123	49	In this study, RNA corresponding to bovine enterovirus (BEV) was detected in 24.6 % of faecal samples (17/69) from diarrheic and healthy cattle in six different areas in China by an RT-PCR screening method. These viruses were characterized molecularly through sequence analysis of complete genomes, and the BHM26 and BJ50 isolates were therefore classified into genotype 2 of the BEV-B cluster. A total of 69 bovine diarrheic and healthy faecal specimens from six different areas were examined by RT-PCR using the primer pair 6424U24 and 7450L24, which cover the highly conserved partial 3D gene and 3 0 UTR of BEVs. The results showed that virus-specific RNA could be detected directly in faecal material in 17 of 69 samples (24.6 %). The results showed that the 3D/3 0 UTR (1026 nt) of BHM26 and BJ50 shared 86 % and 88 % nucleotide sequence identity, respectively, to that of BEV strain Jena38/02 (GenBank accession number: DQ092789), indicating that the two isolates are bovine enterovirus.	./cache/cord-285892-tp6mlqtw.txt	./txt/cord-285892-tp6mlqtw.txt