id author title date pages extension mime words sentences flesch summary cache txt cord-290546-zu5ns4yq Fennestad, K. L. Pleural effusion disease in rabbits. Properties of the aetiological agent 1983 .txt text/plain 3821 403 83 The size and heat sensitivity of Pleural effusion disease (PED) agent or virus (PEDV) propagated in rabbits were examined. PEDV and the Stockholm agent appeared identical concerning pathogenic and immunogenic properties by infection experiments and protection tests in rabbits. The third isolate, obtained from the laboratory, which several years previously had supplied material for demonstration of the Stockholm agent, differed from PEDV in pathogenic and immunogenic properties. Stock PEDV consisted of pooled rabbit sera obtained 48 hours after subcutaneous inoculation with pleural fluid or infectious serum. Stocks of the Stockholm agent and the three isolates were from the first or second rabbit passage, prepared in the same manner as for PEDV. HCV229E and CV Paris were used as antigens in E L I S A to detect antibody rises to coronaviruses in paired sere from 20 rabbits infected with the 5 isolates. ./cache/cord-290546-zu5ns4yq.txt ./txt/cord-290546-zu5ns4yq.txt