id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-293945-gyb9mjb5	Chai, Weidong	Antiviral effects of a probiotic Enterococcus faecium strain against transmissible gastroenteritis coronavirus	2012-11-28		.txt	text/plain	4304	199	41	Virus yield reduction assay ST cell monolayers were infected with TGEV with or without probiotic bacteria treatment according to the experimental design. faecium (1.00E?07 CFU/ml) when the probiotic was added to the cells together with the virus during the infection period (competition assay). faecium inactivates TGEV particles by direct physical interaction with virus, a cell-free preincubation assay was performed. faecium together with the virus significantly increases mRNA expression levels of the pro-inflammatory cytokines interleukin 6 (IL-6) and IL-8 (an approximately 3-and 13-fold increase, respectively) when compared with TGEV-infected ST cells that had not been exposed to the probiotic (Fig. 6) . faecium (pretreatment), the viability of TGEV-infected cells was protected, and virus yields were reduced (Figs. In this study, the competition assay in which virus and probiotic bacteria are present in the culture medium side by side, exhibited the most pronounced antiviral activity in terms of cell survival (Figs.	./cache/cord-293945-gyb9mjb5.txt	./txt/cord-293945-gyb9mjb5.txt