id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-294218-v4gjabp3	Dea, S.	Intracellular synthesis and processing of the structural glycoproteins of turkey enteric coronavirus	1989		.txt	text/plain	5297	256	49	Pulse labeling of cells with [(35)S]methionine or [(3)H]glucosamine at different times after infection, followed by SDS-PAGE and Western immunoblotting analysis using rabbit anti-TCV hyperimmune serum, was used to resolve and identify TCV-induced intracellular proteins. In the present study, we have investigated the intracellular synthesis and post-translational modifications of virus-coded polypeptides in cultures of HRT-18 infected with TCV in the presence or the absence of glycosylation inhibitors. Radioim-munoprecipitation experiments using the anti-TCV hyperimmune serum, after [35S]methionine and [3H]glucosamine labeling, confirmed the absence of the peplomeric glycoproteins from TM-treated cells, whereas the relative amount of the unglycosylated high mol.wt, polypeptide species increased (Fig. 4A, lanes 5 and 7) . In this paper, we described the identification of virus-induced intracellular polypeptides in TCV-infected HRT-18 cells, the kinetic of their synthesis, their post-translational processing in the presence of glycosylation inhibitors and their significance with respect to the production of mature virions.	./cache/cord-294218-v4gjabp3.txt	./txt/cord-294218-v4gjabp3.txt