id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-295409-7l0pglef	Percy, D.	Replication of sialodacryoadenitis virus in mouse L-2 cells	1989		.txt	text/plain	3069	178	54	The ability to propagate SDAV to high titers in the widely available L-2 cell line should promote the study of this virus and facilitate its comparison with other murine coronaviruses. In LBC cells inoculated with strain #681 of SDAV, CPE was first detected in infected cultures 48 hours pi, and syncytia were observed by 72 hours pi [11] . Viral antigen and TCIDs0 titers of 107/0.2ml could be detected in inoculated culture fluid at 24 hours pi by the tenth passage of SDAV in LBC cells [11] . Submandibular and parotid salivary glands from animals infected with the eighth pass of SDAV were homogenized to make a 10% (w/v) suspension in PBS, then 0.5 ml was inoculated onto L-2 or L-929 cells, incubated at 37 °C, and observed for evidence of viral antigen and CPE. Following 5-6 serial passages of supernatant fluid from infected cell cultures, viral antigen, infectious virus, and CPE could be readily demonstrated.	./cache/cord-295409-7l0pglef.txt	./txt/cord-295409-7l0pglef.txt