id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316797-sf2lu45f	Hirano, N.	Replication of rat coronavirus in a rat cell line, LBC	1985		.txt	text/plain	895	61	65	Rat coronavirus readily propagated and induced marked cytopathic effect in a rat cell line, LBC cell culture, which provided a sensitive, practical assay system for viral infectivity and neutralizing antibody, and a satisfactory source of the virus. The growth of RCV has been reported on a primary rat kidney cell culture but not on any established cell lines. This brief communication recommends a rat cell line, LBC, providing propagation of RCV and useful tool for infectivity assay. The culture fluid sampled at ¢8 hours p.i. was assayed for infectivity by inoculating into LBC cells prepared in 13 × 100 mm test tubes, showing an infectivity titer of i07.5 50 per cent tissue culture infective doses (TCIDs0)/0.2 ml. The LBC cell culture might be a much more useful tool for RCV propagation and assay. The LBC cells can also support growth of siModaeryoadenitis virus of rat, strain 681 (1), but not of MHV strains (unpublished observation).	./cache/cord-316797-sf2lu45f.txt	./txt/cord-316797-sf2lu45f.txt