id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-321195-cndq6aqb	Xue, Chunyi	Chimeric influenza-virus-like particles containing the porcine reproductive and respiratory syndrome virus GP5 protein and the influenza virus HA and M1 proteins	2014-07-27		.txt	text/plain	4542	229	49	In this study, we have developed a chimeric virus-like particle (VLP) vaccine candidate for porcine reproductive and respiratory syndrome virus (PRRSV) and H3N2 influenza virus and investigated its immunogenicity in mice. The HA and M1 proteins from the H3N2 influenza virus and the PRRSV GP5 protein fused to the cytoplasmic and transmembrane domains of the NA protein were both incorporated into the chimeric VLPs. Analysis of the immune responses showed that the chimeric VLPs elicited serum antibodies specific for both PRRSV GP5 and the H3N2 HA protein, and they stimulated cellular immune responses compared to the responses to equivalent amounts of inactivated viruses. Serum samples were collected on the 14th, 28th, and 42nd day after primary immunization of mice (8 per group) vaccinated with chimeric VLPs, inactivated H3N2 virus, or PBS. A trivalent virus-like particle vaccine elicits protective immune responses against seasonal influenza strains in mice and ferrets	./cache/cord-321195-cndq6aqb.txt	./txt/cord-321195-cndq6aqb.txt