id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-338641-s006a7m0	Black, W. D.	Reverse transcriptase-polymerase chain reaction for the detection equine rhinitis B viruses and cell culture isolation of the virus	2006-08-24		.txt	text/plain	4042	214	56	title: Reverse transcriptase-polymerase chain reaction for the detection equine rhinitis B viruses and cell culture isolation of the virus The RT-PCR was used to detect viral RNA in six of 17 nasopharyngeal swab samples from horses that had clinical signs of acute febrile respiratory disease but from which ERBV was not initially isolated in cell culture. Virus isolation in cell culture had been attempted for all 17 nasopharyngeal swab samples, but no CPE was visible during three passages on RK13, Vero and EFK cells. The conclusive determination of ERBV as the cause of equine respiratory disease by detection of virus in nasopharyngeal samples by nested RT-PCR is, however, also made difficult by the long-term persistent infection of horses with these viruses without causing apparent disease, as reported after the repeated isolation of ERBV1 over 2 years after two yearling colts were experimentally infected [3] .	./cache/cord-338641-s006a7m0.txt	./txt/cord-338641-s006a7m0.txt