id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-345940-adg264vb	Wanitchang, Asawin	Characterization of influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus	2018-08-22		.txt	text/plain	5030	262	54	In this study, we constructed scIAV-S, a single-cycle influenza A virus pseudotyped with the spike protein of porcine epidemic diarrhea virus (PEDV), and demonstrated that this virus could infect cultured cells and trigger massive syncytium formation. It should also be noted that control supernatants from HEK293T cells transfected with pCAGGS expressing S AVCT12 alone did not yield detectable syncytium formation in VeroE6-APN cells, suggesting that the detected syncytia were not due to PEDV-S expressed from residual plasmids (data not shown). To further test whether the mCherry expression in infected cells required active influenza A virus polymerase activity, we attempted to construct scIAV-S AVCT12 by omitting the plasmid encoding the PB2 polymerase and examined its infectivity in VeroE6-APN cells. Likewise, VeroE6-APN cells treated with NH 4 Cl (50 mM for 2 h) to neutralize the intracellular pH also displayed strong mCherry expression and extensive syncytium formation when infected with scIAV-S AVCT12 (Fig. 3A) .	./cache/cord-345940-adg264vb.txt	./txt/cord-345940-adg264vb.txt