id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346928-g1dqiki6	Costantini, V.	Respiratory and fecal shedding of Porcine respiratory coronavirus (PRCV) in sentinel weaned pigs and sequence of the partial S-gene of the PRCV isolates	2003-11-26		.txt	text/plain	5893	255	55	Porcine respiratory coronavirus (PRCV), a spike (S) gene deletion mutant of Transmissible gastroenteritis virus (TGEV), causes mild or subclinical respiratory infections in pigs. In preliminary studies, inoculation of gnotobiotic pigs with each plaque-purified pair of the nasal and fecal PRCV isolates, revealed no clinical disease but different tropisms. Fecal and nasal swabs from the field cases (sentinel pigs), cell culture isolates and fecal and nasal swabs from gnotobiotic pigs were tested by nested-RT-PCR to detect and to differentiate TGEV and PRCV viral RNA as previously described by Kim et al. Fecal and nasal swab supernatant fluids from the sentinel field cases or the cell culture passaged PRCV isolates and nasal and fecal swabs from gnotobiotic pigs were diluted in minimum essential medium (MEM-E) and tested by CCIF to detect infectious virus using previously described procedures [23] .	./cache/cord-346928-g1dqiki6.txt	./txt/cord-346928-g1dqiki6.txt