id	sid	tid	token	lemma	pos
cord-004738-vnz15x84	1	1	key	key	JJ
cord-004738-vnz15x84	1	2	:	:	:
cord-004738-vnz15x84	1	3	cord-004738-vnz15x84	cord-004738-vnz15x84	JJ
cord-004738-vnz15x84	1	4	authors	author	NNS
cord-004738-vnz15x84	1	5	:	:	:
cord-004738-vnz15x84	1	6	Chen	Chen	NNP
cord-004738-vnz15x84	1	7	,	,	,
cord-004738-vnz15x84	1	8	H.	H.	NNP
cord-004738-vnz15x84	1	9	H.	H.	NNP
cord-004738-vnz15x84	1	10	;	;	:
cord-004738-vnz15x84	1	11	Stark	Stark	NNP
cord-004738-vnz15x84	1	12	,	,	,
cord-004738-vnz15x84	1	13	C.	C.	NNP
cord-004738-vnz15x84	1	14	J.	J.	NNP
cord-004738-vnz15x84	1	15	;	;	:
cord-004738-vnz15x84	1	16	Atreya	Atreya	NNP
cord-004738-vnz15x84	1	17	,	,	,
cord-004738-vnz15x84	1	18	C.	C.	NNP
cord-004738-vnz15x84	1	19	D.	D.	NNP
cord-004738-vnz15x84	2	1	title	title	NN
cord-004738-vnz15x84	2	2	:	:	:
cord-004738-vnz15x84	3	1	The	the	DT
cord-004738-vnz15x84	3	2	rubella	rubella	NN
cord-004738-vnz15x84	3	3	virus	virus	NN
cord-004738-vnz15x84	3	4	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	3	5	protease	protease	NN
cord-004738-vnz15x84	3	6	recognizes	recognize	VBZ
cord-004738-vnz15x84	3	7	itself	-PRON-	PRP
cord-004738-vnz15x84	3	8	via	via	IN
cord-004738-vnz15x84	3	9	an	an	DT
cord-004738-vnz15x84	3	10	internal	internal	JJ
cord-004738-vnz15x84	3	11	sequence	sequence	NN
cord-004738-vnz15x84	3	12	present	present	JJ
cord-004738-vnz15x84	3	13	upstream	upstream	RB
cord-004738-vnz15x84	3	14	of	of	IN
cord-004738-vnz15x84	3	15	the	the	DT
cord-004738-vnz15x84	3	16	cleavage	cleavage	NN
cord-004738-vnz15x84	3	17	site	site	NN
cord-004738-vnz15x84	3	18	for	for	IN
cord-004738-vnz15x84	3	19	trans	trans	NN
cord-004738-vnz15x84	3	20	-	-	NN
cord-004738-vnz15x84	3	21	activity	activity	NN
cord-004738-vnz15x84	3	22	date	date	NN
cord-004738-vnz15x84	4	1	:	:	:
cord-004738-vnz15x84	4	2	2006	2006	CD
cord-004738-vnz15x84	4	3	-	-	HYPH
cord-004738-vnz15x84	4	4	03	03	CD
cord-004738-vnz15x84	4	5	-	-	HYPH
cord-004738-vnz15x84	4	6	27	27	CD
cord-004738-vnz15x84	4	7	journal	journal	NN
cord-004738-vnz15x84	4	8	:	:	:
cord-004738-vnz15x84	5	1	Arch	Arch	NNP
cord-004738-vnz15x84	5	2	Virol	Virol	NNP
cord-004738-vnz15x84	6	1	DOI	DOI	NNP
cord-004738-vnz15x84	6	2	:	:	:
cord-004738-vnz15x84	6	3	10.1007	10.1007	CD
cord-004738-vnz15x84	6	4	/	/	SYM
cord-004738-vnz15x84	6	5	s00705	s00705	CD
cord-004738-vnz15x84	6	6	-	-	HYPH
cord-004738-vnz15x84	6	7	006	006	CD
cord-004738-vnz15x84	6	8	-	-	HYPH
cord-004738-vnz15x84	6	9	0744	0744	CD
cord-004738-vnz15x84	6	10	-	-	SYM
cord-004738-vnz15x84	6	11	9	9	CD
cord-004738-vnz15x84	6	12	sha	sha	NN
cord-004738-vnz15x84	6	13	:	:	:
cord-004738-vnz15x84	6	14	7b242f0ac81dd1f79d0c471df13f90426b0383bd	7b242f0ac81dd1f79d0c471df13f90426b0383bd	CD
cord-004738-vnz15x84	7	1	doc_id	doc_id	CD
cord-004738-vnz15x84	7	2	:	:	:
cord-004738-vnz15x84	7	3	4738	4738	CD
cord-004738-vnz15x84	7	4	cord_uid	cord_uid	NNS
cord-004738-vnz15x84	7	5	:	:	:
cord-004738-vnz15x84	8	1	vnz15x84	vnz15x84	NNP
cord-004738-vnz15x84	9	1	The	the	DT
cord-004738-vnz15x84	9	2	substrate	substrate	JJ
cord-004738-vnz15x84	9	3	requirement	requirement	NN
cord-004738-vnz15x84	9	4	for	for	IN
cord-004738-vnz15x84	9	5	rubella	rubella	NN
cord-004738-vnz15x84	9	6	virus	virus	NN
cord-004738-vnz15x84	9	7	protease	protease	NN
cord-004738-vnz15x84	9	8	trans	trans	NN
cord-004738-vnz15x84	9	9	-	-	NN
cord-004738-vnz15x84	9	10	activity	activity	NN
cord-004738-vnz15x84	9	11	is	be	VBZ
cord-004738-vnz15x84	9	12	unknown	unknown	JJ
cord-004738-vnz15x84	9	13	.	.	.
cord-004738-vnz15x84	10	1	Here	here	RB
cord-004738-vnz15x84	10	2	,	,	,
cord-004738-vnz15x84	10	3	we	-PRON-	PRP
cord-004738-vnz15x84	10	4	analyzed	analyze	VBD
cord-004738-vnz15x84	10	5	the	the	DT
cord-004738-vnz15x84	10	6	cleavability	cleavability	NN
cord-004738-vnz15x84	10	7	of	of	IN
cord-004738-vnz15x84	10	8	RV	rv	NN
cord-004738-vnz15x84	10	9	P200-derived	p200-derived	JJ
cord-004738-vnz15x84	10	10	substrates	substrate	NNS
cord-004738-vnz15x84	10	11	varying	vary	VBG
cord-004738-vnz15x84	10	12	in	in	IN
cord-004738-vnz15x84	10	13	their	-PRON-	PRP$
cord-004738-vnz15x84	10	14	N	n	CD
cord-004738-vnz15x84	10	15	-	-	HYPH
cord-004738-vnz15x84	10	16	terminal	terminal	NN
cord-004738-vnz15x84	10	17	lengths	length	NNS
cord-004738-vnz15x84	10	18	(	(	-LRB-
cord-004738-vnz15x84	10	19	72–475	72–475	CD
cord-004738-vnz15x84	10	20	amino	amino	JJ
cord-004738-vnz15x84	10	21	acids	acid	NNS
cord-004738-vnz15x84	10	22	)	)	-RRB-
cord-004738-vnz15x84	10	23	from	from	IN
cord-004738-vnz15x84	10	24	the	the	DT
cord-004738-vnz15x84	10	25	cleavage	cleavage	NN
cord-004738-vnz15x84	10	26	site	site	NN
cord-004738-vnz15x84	10	27	by	by	IN
cord-004738-vnz15x84	10	28	the	the	DT
cord-004738-vnz15x84	10	29	RV	RV	NNP
cord-004738-vnz15x84	10	30	protease	protease	NN
cord-004738-vnz15x84	10	31	trans	trans	NN
cord-004738-vnz15x84	10	32	-	-	NN
cord-004738-vnz15x84	10	33	activity	activity	NN
cord-004738-vnz15x84	10	34	.	.	.
cord-004738-vnz15x84	11	1	Only	only	RB
cord-004738-vnz15x84	11	2	substrates	substrate	NNS
cord-004738-vnz15x84	11	3	with	with	IN
cord-004738-vnz15x84	11	4	at	at	RB
cord-004738-vnz15x84	11	5	least	least	RBS
cord-004738-vnz15x84	11	6	309	309	CD
cord-004738-vnz15x84	11	7	amino	amino	NN
cord-004738-vnz15x84	11	8	acid	acid	NN
cord-004738-vnz15x84	11	9	residues	residue	NNS
cord-004738-vnz15x84	11	10	N	n	NN
cord-004738-vnz15x84	11	11	-	-	HYPH
cord-004738-vnz15x84	11	12	terminal	terminal	NN
cord-004738-vnz15x84	11	13	to	to	IN
cord-004738-vnz15x84	11	14	the	the	DT
cord-004738-vnz15x84	11	15	cleavage	cleavage	NN
cord-004738-vnz15x84	11	16	site	site	NN
cord-004738-vnz15x84	11	17	were	be	VBD
cord-004738-vnz15x84	11	18	able	able	JJ
cord-004738-vnz15x84	11	19	to	to	TO
cord-004738-vnz15x84	11	20	undergo	undergo	VB
cord-004738-vnz15x84	11	21	cleavage	cleavage	NN
cord-004738-vnz15x84	11	22	.	.	.
cord-004738-vnz15x84	12	1	Further	further	RB
cord-004738-vnz15x84	12	2	,	,	,
cord-004738-vnz15x84	12	3	rubella	rubella	NN
cord-004738-vnz15x84	12	4	sequence	sequence	NN
cord-004738-vnz15x84	12	5	was	be	VBD
cord-004738-vnz15x84	12	6	found	find	VBN
cord-004738-vnz15x84	12	7	to	to	TO
cord-004738-vnz15x84	12	8	be	be	VB
cord-004738-vnz15x84	12	9	necessary	necessary	JJ
cord-004738-vnz15x84	12	10	in	in	IN
cord-004738-vnz15x84	12	11	the	the	DT
cord-004738-vnz15x84	12	12	N	n	CD
cord-004738-vnz15x84	12	13	-	-	HYPH
cord-004738-vnz15x84	12	14	terminal	terminal	NN
cord-004738-vnz15x84	12	15	region	region	NN
cord-004738-vnz15x84	12	16	of	of	IN
cord-004738-vnz15x84	12	17	the	the	DT
cord-004738-vnz15x84	12	18	substrate	substrate	NN
cord-004738-vnz15x84	12	19	,	,	,
cord-004738-vnz15x84	12	20	whereas	whereas	IN
cord-004738-vnz15x84	12	21	a	a	DT
cord-004738-vnz15x84	12	22	heterologous	heterologous	JJ
cord-004738-vnz15x84	12	23	sequence	sequence	NN
cord-004738-vnz15x84	12	24	C	c	NN
cord-004738-vnz15x84	12	25	-	-	HYPH
cord-004738-vnz15x84	12	26	terminal	terminal	NN
cord-004738-vnz15x84	12	27	to	to	IN
cord-004738-vnz15x84	12	28	the	the	DT
cord-004738-vnz15x84	12	29	cleavage	cleavage	NN
cord-004738-vnz15x84	12	30	site	site	NN
cord-004738-vnz15x84	12	31	was	be	VBD
cord-004738-vnz15x84	12	32	tolerated	tolerate	VBN
cord-004738-vnz15x84	12	33	.	.	.
cord-004738-vnz15x84	13	1	These	these	DT
cord-004738-vnz15x84	13	2	results	result	NNS
cord-004738-vnz15x84	13	3	demonstrated	demonstrate	VBD
cord-004738-vnz15x84	13	4	a	a	DT
cord-004738-vnz15x84	13	5	requirement	requirement	NN
cord-004738-vnz15x84	13	6	for	for	IN
cord-004738-vnz15x84	13	7	residues	residue	NNS
cord-004738-vnz15x84	13	8	located	locate	VBN
cord-004738-vnz15x84	13	9	between	between	IN
cord-004738-vnz15x84	13	10	amino	amino	NN
cord-004738-vnz15x84	13	11	acids	acid	NNS
cord-004738-vnz15x84	13	12	994–1102	994–1102	CD
cord-004738-vnz15x84	13	13	of	of	IN
cord-004738-vnz15x84	13	14	the	the	DT
cord-004738-vnz15x84	13	15	RV	RV	NNP
cord-004738-vnz15x84	13	16	P200	p200	NN
cord-004738-vnz15x84	13	17	polyprotein	polyprotein	NN
cord-004738-vnz15x84	13	18	,	,	,
cord-004738-vnz15x84	13	19	besides	besides	IN
cord-004738-vnz15x84	13	20	its	-PRON-	PRP$
cord-004738-vnz15x84	13	21	cleavage	cleavage	NN
cord-004738-vnz15x84	13	22	site	site	NN
cord-004738-vnz15x84	13	23	for	for	IN
cord-004738-vnz15x84	13	24	RV	rv	NN
cord-004738-vnz15x84	13	25	protease	protease	NN
cord-004738-vnz15x84	13	26	trans	trans	NN
cord-004738-vnz15x84	13	27	-	-	NN
cord-004738-vnz15x84	13	28	activity	activity	NN
cord-004738-vnz15x84	13	29	.	.	.
cord-004738-vnz15x84	14	1	This	this	DT
cord-004738-vnz15x84	14	2	region	region	NN
cord-004738-vnz15x84	14	3	overlaps	overlap	VBZ
cord-004738-vnz15x84	14	4	with	with	IN
cord-004738-vnz15x84	14	5	the	the	DT
cord-004738-vnz15x84	14	6	starting	starting	NN
cord-004738-vnz15x84	14	7	site	site	NN
cord-004738-vnz15x84	14	8	of	of	IN
cord-004738-vnz15x84	14	9	the	the	DT
cord-004738-vnz15x84	14	10	essential	essential	JJ
cord-004738-vnz15x84	14	11	cis	cis	JJ
cord-004738-vnz15x84	14	12	-	-	HYPH
cord-004738-vnz15x84	14	13	protease	protease	JJ
cord-004738-vnz15x84	14	14	activity	activity	NN
cord-004738-vnz15x84	14	15	of	of	IN
cord-004738-vnz15x84	14	16	RV	RV	NNP
cord-004738-vnz15x84	14	17	P200	P200	NNP
cord-004738-vnz15x84	14	18	polyprotein	polyprotein	NN
cord-004738-vnz15x84	14	19	.	.	.
cord-004738-vnz15x84	15	1	This	this	DT
cord-004738-vnz15x84	15	2	is	be	VBZ
cord-004738-vnz15x84	15	3	a	a	DT
cord-004738-vnz15x84	15	4	novel	novel	JJ
cord-004738-vnz15x84	15	5	observation	observation	NN
cord-004738-vnz15x84	15	6	for	for	IN
cord-004738-vnz15x84	15	7	a	a	DT
cord-004738-vnz15x84	15	8	viral	viral	JJ
cord-004738-vnz15x84	15	9	protease	protease	NN
cord-004738-vnz15x84	15	10	of	of	IN
cord-004738-vnz15x84	15	11	the	the	DT
cord-004738-vnz15x84	15	12	family	family	NN
cord-004738-vnz15x84	15	13	Togaviridae	Togaviridae	NNP
cord-004738-vnz15x84	15	14	.	.	.
cord-004738-vnz15x84	16	1	A	a	DT
cord-004738-vnz15x84	16	2	number	number	NN
cord-004738-vnz15x84	16	3	of	of	IN
cord-004738-vnz15x84	16	4	positive	positive	JJ
cord-004738-vnz15x84	16	5	-	-	HYPH
cord-004738-vnz15x84	16	6	stranded	strand	VBN
cord-004738-vnz15x84	16	7	RNA	RNA	NNP
cord-004738-vnz15x84	16	8	viruses	virus	NNS
cord-004738-vnz15x84	16	9	employ	employ	VBP
cord-004738-vnz15x84	16	10	viral	viral	JJ
cord-004738-vnz15x84	16	11	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	16	12	polyprotein	polyprotein	NN
cord-004738-vnz15x84	16	13	processing	processing	NN
cord-004738-vnz15x84	16	14	as	as	IN
cord-004738-vnz15x84	16	15	a	a	DT
cord-004738-vnz15x84	16	16	strategy	strategy	NN
cord-004738-vnz15x84	16	17	for	for	IN
cord-004738-vnz15x84	16	18	genome	genome	NN
cord-004738-vnz15x84	16	19	expression	expression	NN
cord-004738-vnz15x84	16	20	by	by	IN
cord-004738-vnz15x84	16	21	encoding	encode	VBG
cord-004738-vnz15x84	16	22	their	-PRON-	PRP$
cord-004738-vnz15x84	16	23	own	own	JJ
cord-004738-vnz15x84	16	24	(	(	-LRB-
cord-004738-vnz15x84	16	25	viral	viral	JJ
cord-004738-vnz15x84	16	26	)	)	-RRB-
cord-004738-vnz15x84	16	27	proteases	protease	NNS
cord-004738-vnz15x84	16	28	[	[	-LRB-
cord-004738-vnz15x84	16	29	5	5	CD
cord-004738-vnz15x84	16	30	,	,	,
cord-004738-vnz15x84	16	31	7	7	CD
cord-004738-vnz15x84	16	32	,	,	,
cord-004738-vnz15x84	16	33	14	14	CD
cord-004738-vnz15x84	16	34	]	]	-RRB-
cord-004738-vnz15x84	16	35	.	.	.
cord-004738-vnz15x84	17	1	Thus	thus	RB
cord-004738-vnz15x84	17	2	,	,	,
cord-004738-vnz15x84	17	3	in	in	IN
cord-004738-vnz15x84	17	4	order	order	NN
cord-004738-vnz15x84	17	5	to	to	TO
cord-004738-vnz15x84	17	6	achieve	achieve	VB
cord-004738-vnz15x84	17	7	the	the	DT
cord-004738-vnz15x84	17	8	expression	expression	NN
cord-004738-vnz15x84	17	9	of	of	IN
cord-004738-vnz15x84	17	10	multiple	multiple	JJ
cord-004738-vnz15x84	17	11	proteins	protein	NNS
cord-004738-vnz15x84	17	12	from	from	IN
cord-004738-vnz15x84	17	13	a	a	DT
cord-004738-vnz15x84	17	14	single	single	JJ
cord-004738-vnz15x84	17	15	message	message	NN
cord-004738-vnz15x84	17	16	that	that	WDT
cord-004738-vnz15x84	17	17	are	be	VBP
cord-004738-vnz15x84	17	18	essential	essential	JJ
cord-004738-vnz15x84	17	19	for	for	IN
cord-004738-vnz15x84	17	20	regulation	regulation	NN
cord-004738-vnz15x84	17	21	of	of	IN
cord-004738-vnz15x84	17	22	viral	viral	JJ
cord-004738-vnz15x84	17	23	replication	replication	NN
cord-004738-vnz15x84	17	24	and	and	CC
cord-004738-vnz15x84	17	25	biogenesis	biogenesis	NN
cord-004738-vnz15x84	17	26	,	,	,
cord-004738-vnz15x84	17	27	proteolysis	proteolysis	NN
cord-004738-vnz15x84	17	28	of	of	IN
cord-004738-vnz15x84	17	29	the	the	DT
cord-004738-vnz15x84	17	30	viral	viral	JJ
cord-004738-vnz15x84	17	31	polyprotein	polyprotein	JJ
cord-004738-vnz15x84	17	32	precursor	precursor	NN
cord-004738-vnz15x84	17	33	is	be	VBZ
cord-004738-vnz15x84	17	34	an	an	DT
cord-004738-vnz15x84	17	35	essential	essential	JJ
cord-004738-vnz15x84	17	36	event	event	NN
cord-004738-vnz15x84	17	37	in	in	IN
cord-004738-vnz15x84	17	38	most	most	JJS
cord-004738-vnz15x84	17	39	of	of	IN
cord-004738-vnz15x84	17	40	the	the	DT
cord-004738-vnz15x84	17	41	positive	positive	JJ
cord-004738-vnz15x84	17	42	-	-	HYPH
cord-004738-vnz15x84	17	43	strand	strand	NN
cord-004738-vnz15x84	17	44	RNA	RNA	NNP
cord-004738-vnz15x84	17	45	viruses	virus	NNS
cord-004738-vnz15x84	17	46	[	[	-LRB-
cord-004738-vnz15x84	17	47	14	14	CD
cord-004738-vnz15x84	17	48	,	,	,
cord-004738-vnz15x84	17	49	16	16	CD
cord-004738-vnz15x84	17	50	]	]	-RRB-
cord-004738-vnz15x84	17	51	.	.	.
cord-004738-vnz15x84	18	1	This	this	DT
cord-004738-vnz15x84	18	2	is	be	VBZ
cord-004738-vnz15x84	18	3	in	in	IN
cord-004738-vnz15x84	18	4	contrast	contrast	NN
cord-004738-vnz15x84	18	5	to	to	IN
cord-004738-vnz15x84	18	6	the	the	DT
cord-004738-vnz15x84	18	7	mRNAs	mrnas	CD
cord-004738-vnz15x84	18	8	of	of	IN
cord-004738-vnz15x84	18	9	their	-PRON-	PRP$
cord-004738-vnz15x84	18	10	eukaryotic	eukaryotic	JJ
cord-004738-vnz15x84	18	11	host	host	NN
cord-004738-vnz15x84	18	12	cells	cell	NNS
cord-004738-vnz15x84	18	13	,	,	,
cord-004738-vnz15x84	18	14	which	which	WDT
cord-004738-vnz15x84	18	15	mostly	mostly	RB
cord-004738-vnz15x84	18	16	code	code	NN
cord-004738-vnz15x84	18	17	for	for	IN
cord-004738-vnz15x84	18	18	single	single	JJ
cord-004738-vnz15x84	18	19	proteins	protein	NNS
cord-004738-vnz15x84	18	20	[	[	-LRB-
cord-004738-vnz15x84	18	21	16	16	CD
cord-004738-vnz15x84	18	22	]	]	-RRB-
cord-004738-vnz15x84	18	23	.	.	.
cord-004738-vnz15x84	19	1	In	in	IN
cord-004738-vnz15x84	19	2	alphaviruses	alphavirus	NNS
cord-004738-vnz15x84	19	3	and	and	CC
cord-004738-vnz15x84	19	4	rubella	rubella	NN
cord-004738-vnz15x84	19	5	virus	virus	NN
cord-004738-vnz15x84	19	6	,	,	,
cord-004738-vnz15x84	19	7	members	member	NNS
cord-004738-vnz15x84	19	8	of	of	IN
cord-004738-vnz15x84	19	9	the	the	DT
cord-004738-vnz15x84	19	10	family	family	NN
cord-004738-vnz15x84	19	11	Togaviridae	Togaviridae	NNP
cord-004738-vnz15x84	19	12	,	,	,
cord-004738-vnz15x84	19	13	two	two	CD
cord-004738-vnz15x84	19	14	polyproteins	polyprotein	NNS
cord-004738-vnz15x84	19	15	are	be	VBP
cord-004738-vnz15x84	19	16	expressed	express	VBN
cord-004738-vnz15x84	19	17	:	:	:
cord-004738-vnz15x84	19	18	the	the	DT
cord-004738-vnz15x84	19	19	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	19	20	polyprotein	polyprotein	NN
cord-004738-vnz15x84	19	21	,	,	,
cord-004738-vnz15x84	19	22	directly	directly	RB
cord-004738-vnz15x84	19	23	expressed	express	VBN
cord-004738-vnz15x84	19	24	from	from	IN
cord-004738-vnz15x84	19	25	the	the	DT
cord-004738-vnz15x84	19	26	genomic	genomic	JJ
cord-004738-vnz15x84	19	27	RNA	RNA	NNP
cord-004738-vnz15x84	19	28	,	,	,
cord-004738-vnz15x84	19	29	and	and	CC
cord-004738-vnz15x84	19	30	another	another	DT
cord-004738-vnz15x84	19	31	polyprotein	polyprotein	NN
cord-004738-vnz15x84	19	32	,	,	,
cord-004738-vnz15x84	19	33	expressed	express	VBD
cord-004738-vnz15x84	19	34	from	from	IN
cord-004738-vnz15x84	19	35	a	a	DT
cord-004738-vnz15x84	19	36	sub	sub	JJ
cord-004738-vnz15x84	19	37	-	-	JJ
cord-004738-vnz15x84	19	38	genomic	genomic	JJ
cord-004738-vnz15x84	19	39	mRNA	mrna	NN
cord-004738-vnz15x84	19	40	synthesized	synthesize	VBN
cord-004738-vnz15x84	19	41	during	during	IN
cord-004738-vnz15x84	19	42	the	the	DT
cord-004738-vnz15x84	19	43	viral	viral	JJ
cord-004738-vnz15x84	19	44	infection	infection	NN
cord-004738-vnz15x84	19	45	[	[	-LRB-
cord-004738-vnz15x84	19	46	16	16	CD
cord-004738-vnz15x84	19	47	]	]	-RRB-
cord-004738-vnz15x84	19	48	.	.	.
cord-004738-vnz15x84	20	1	Rubella	rubella	NN
cord-004738-vnz15x84	20	2	virus	virus	NN
cord-004738-vnz15x84	20	3	is	be	VBZ
cord-004738-vnz15x84	20	4	an	an	DT
cord-004738-vnz15x84	20	5	enveloped	envelop	VBN
cord-004738-vnz15x84	20	6	virus	virus	NN
cord-004738-vnz15x84	20	7	of	of	IN
cord-004738-vnz15x84	20	8	the	the	DT
cord-004738-vnz15x84	20	9	genus	genus	NN
cord-004738-vnz15x84	20	10	Rubivirus	Rubivirus	NNP
cord-004738-vnz15x84	20	11	in	in	IN
cord-004738-vnz15x84	20	12	the	the	DT
cord-004738-vnz15x84	20	13	family	family	NN
cord-004738-vnz15x84	20	14	Togaviridae	Togaviridae	NNP
cord-004738-vnz15x84	20	15	[	[	-LRB-
cord-004738-vnz15x84	20	16	2	2	CD
cord-004738-vnz15x84	20	17	]	]	-RRB-
cord-004738-vnz15x84	20	18	.	.	.
cord-004738-vnz15x84	21	1	The	the	DT
cord-004738-vnz15x84	21	2	virion	virion	NN
cord-004738-vnz15x84	21	3	has	have	VBZ
cord-004738-vnz15x84	21	4	a	a	DT
cord-004738-vnz15x84	21	5	40S	40s	CD
cord-004738-vnz15x84	21	6	(	(	-LRB-
cord-004738-vnz15x84	21	7	9762	9762	CD
cord-004738-vnz15x84	21	8	nucleotide	nucleotide	JJ
cord-004738-vnz15x84	21	9	long	long	JJ
cord-004738-vnz15x84	21	10	)	)	-RRB-
cord-004738-vnz15x84	21	11	single	single	RB
cord-004738-vnz15x84	21	12	-	-	HYPH
cord-004738-vnz15x84	21	13	stranded	stranded	JJ
cord-004738-vnz15x84	21	14	,	,	,
cord-004738-vnz15x84	21	15	positive	positive	JJ
cord-004738-vnz15x84	21	16	-	-	HYPH
cord-004738-vnz15x84	21	17	sense	sense	NN
cord-004738-vnz15x84	21	18	,	,	,
cord-004738-vnz15x84	21	19	polyadenylated	polyadenylate	VBN
cord-004738-vnz15x84	21	20	RNA	RNA	NNP
cord-004738-vnz15x84	21	21	genome	genome	NN
cord-004738-vnz15x84	21	22	,	,	,
cord-004738-vnz15x84	21	23	which	which	WDT
cord-004738-vnz15x84	21	24	serves	serve	VBZ
cord-004738-vnz15x84	21	25	as	as	IN
cord-004738-vnz15x84	21	26	the	the	DT
cord-004738-vnz15x84	21	27	mRNA	mrna	NN
cord-004738-vnz15x84	21	28	following	follow	VBG
cord-004738-vnz15x84	21	29	a	a	DT
cord-004738-vnz15x84	21	30	series	series	NN
cord-004738-vnz15x84	21	31	of	of	IN
cord-004738-vnz15x84	21	32	events	event	NNS
cord-004738-vnz15x84	21	33	that	that	WDT
cord-004738-vnz15x84	21	34	lead	lead	VBP
cord-004738-vnz15x84	21	35	to	to	IN
cord-004738-vnz15x84	21	36	the	the	DT
cord-004738-vnz15x84	21	37	uncoating	uncoating	NN
cord-004738-vnz15x84	21	38	of	of	IN
cord-004738-vnz15x84	21	39	the	the	DT
cord-004738-vnz15x84	21	40	viral	viral	JJ
cord-004738-vnz15x84	21	41	particle	particle	NN
cord-004738-vnz15x84	21	42	upon	upon	IN
cord-004738-vnz15x84	21	43	entry	entry	NN
cord-004738-vnz15x84	21	44	into	into	IN
cord-004738-vnz15x84	21	45	cells	cell	NNS
cord-004738-vnz15x84	21	46	[	[	-LRB-
cord-004738-vnz15x84	21	47	1	1	CD
cord-004738-vnz15x84	21	48	,	,	,
cord-004738-vnz15x84	21	49	2	2	CD
cord-004738-vnz15x84	21	50	,	,	,
cord-004738-vnz15x84	21	51	4	4	CD
cord-004738-vnz15x84	21	52	]	]	-RRB-
cord-004738-vnz15x84	21	53	.	.	.
cord-004738-vnz15x84	22	1	In	in	IN
cord-004738-vnz15x84	22	2	an	an	DT
cord-004738-vnz15x84	22	3	infected	infected	JJ
cord-004738-vnz15x84	22	4	cell	cell	NN
cord-004738-vnz15x84	22	5	,	,	,
cord-004738-vnz15x84	22	6	RV	RV	NNP
cord-004738-vnz15x84	22	7	mRNA	mrna	NN
cord-004738-vnz15x84	22	8	first	first	RB
cord-004738-vnz15x84	22	9	translates	translate	VBZ
cord-004738-vnz15x84	22	10	into	into	IN
cord-004738-vnz15x84	22	11	a	a	DT
cord-004738-vnz15x84	22	12	P200	p200	NN
cord-004738-vnz15x84	22	13	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	22	14	polypeptide	polypeptide	NN
cord-004738-vnz15x84	22	15	[	[	-LRB-
cord-004738-vnz15x84	22	16	2	2	CD
cord-004738-vnz15x84	22	17	]	]	-RRB-
cord-004738-vnz15x84	22	18	.	.	.
cord-004738-vnz15x84	23	1	Newly	newly	RB
cord-004738-vnz15x84	23	2	synthesized	synthesize	VBN
cord-004738-vnz15x84	23	3	P200	p200	NN
cord-004738-vnz15x84	23	4	often	often	RB
cord-004738-vnz15x84	23	5	undergoes	undergo	VBZ
cord-004738-vnz15x84	23	6	ciscleavage	ciscleavage	NN
cord-004738-vnz15x84	23	7	at	at	IN
cord-004738-vnz15x84	23	8	NH	NH	NNP
cord-004738-vnz15x84	23	9	2	2	CD
cord-004738-vnz15x84	23	10	-SRGG	-srgg	SYM
cord-004738-vnz15x84	23	11	1301/1302	1301/1302	CD
cord-004738-vnz15x84	23	12	G	g	NN
cord-004738-vnz15x84	23	13	-	-	HYPH
cord-004738-vnz15x84	23	14	COOH	COOH	NNP
cord-004738-vnz15x84	23	15	into	into	IN
cord-004738-vnz15x84	23	16	two	two	CD
cord-004738-vnz15x84	23	17	mature	mature	JJ
cord-004738-vnz15x84	23	18	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	23	19	proteins	protein	NNS
cord-004738-vnz15x84	23	20	,	,	,
cord-004738-vnz15x84	23	21	P150	P150	NNP
cord-004738-vnz15x84	23	22	and	and	CC
cord-004738-vnz15x84	23	23	P90	P90	NNP
cord-004738-vnz15x84	23	24	,	,	,
cord-004738-vnz15x84	23	25	by	by	IN
cord-004738-vnz15x84	23	26	a	a	DT
cord-004738-vnz15x84	23	27	protease	protease	NN
cord-004738-vnz15x84	23	28	domain	domain	NN
cord-004738-vnz15x84	23	29	contained	contain	VBN
cord-004738-vnz15x84	23	30	within	within	IN
cord-004738-vnz15x84	23	31	the	the	DT
cord-004738-vnz15x84	23	32	C	C	NNP
cord-004738-vnz15x84	23	33	-	-	HYPH
cord-004738-vnz15x84	23	34	terminal	terminal	NN
cord-004738-vnz15x84	23	35	region	region	NN
cord-004738-vnz15x84	23	36	of	of	IN
cord-004738-vnz15x84	23	37	P150	P150	NNP
cord-004738-vnz15x84	23	38	[	[	-LRB-
cord-004738-vnz15x84	23	39	3	3	CD
cord-004738-vnz15x84	23	40	,	,	,
cord-004738-vnz15x84	23	41	9	9	CD
cord-004738-vnz15x84	23	42	,	,	,
cord-004738-vnz15x84	23	43	11	11	CD
cord-004738-vnz15x84	23	44	,	,	,
cord-004738-vnz15x84	23	45	18	18	CD
cord-004738-vnz15x84	23	46	]	]	-RRB-
cord-004738-vnz15x84	23	47	.	.	.
cord-004738-vnz15x84	24	1	The	the	DT
cord-004738-vnz15x84	24	2	viral	viral	JJ
cord-004738-vnz15x84	24	3	P200	p200	NN
cord-004738-vnz15x84	24	4	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	24	5	polyprotein	polyprotein	NNP
cord-004738-vnz15x84	24	6	contains	contain	VBZ
cord-004738-vnz15x84	24	7	four	four	CD
cord-004738-vnz15x84	24	8	conserved	conserved	JJ
cord-004738-vnz15x84	24	9	functional	functional	JJ
cord-004738-vnz15x84	24	10	domains	domain	NNS
cord-004738-vnz15x84	24	11	that	that	WDT
cord-004738-vnz15x84	24	12	are	be	VBP
cord-004738-vnz15x84	24	13	involved	involve	VBN
cord-004738-vnz15x84	24	14	in	in	IN
cord-004738-vnz15x84	24	15	viral	viral	JJ
cord-004738-vnz15x84	24	16	RNA	RNA	NNP
cord-004738-vnz15x84	24	17	synthesis	synthesis	NN
cord-004738-vnz15x84	24	18	and	and	CC
cord-004738-vnz15x84	24	19	replication	replication	NN
cord-004738-vnz15x84	24	20	.	.	.
cord-004738-vnz15x84	25	1	They	-PRON-	PRP
cord-004738-vnz15x84	25	2	are	be	VBP
cord-004738-vnz15x84	25	3	sequentially	sequentially	RB
cord-004738-vnz15x84	25	4	located	locate	VBN
cord-004738-vnz15x84	25	5	from	from	IN
cord-004738-vnz15x84	25	6	the	the	DT
cord-004738-vnz15x84	25	7	N	n	CD
cord-004738-vnz15x84	25	8	terminus	terminus	NN
cord-004738-vnz15x84	25	9	to	to	IN
cord-004738-vnz15x84	25	10	the	the	DT
cord-004738-vnz15x84	25	11	C	C	NNP
cord-004738-vnz15x84	25	12	terminus	terminus	NN
cord-004738-vnz15x84	25	13	,	,	,
cord-004738-vnz15x84	25	14	as	as	IN
cord-004738-vnz15x84	25	15	methyltransferase	methyltransferase	NN
cord-004738-vnz15x84	25	16	,	,	,
cord-004738-vnz15x84	25	17	protease	protease	NN
cord-004738-vnz15x84	25	18	,	,	,
cord-004738-vnz15x84	25	19	helicase	helicase	NN
cord-004738-vnz15x84	25	20	,	,	,
cord-004738-vnz15x84	25	21	and	and	CC
cord-004738-vnz15x84	25	22	RNA	RNA	NNP
cord-004738-vnz15x84	25	23	-	-	HYPH
cord-004738-vnz15x84	25	24	dependent	dependent	JJ
cord-004738-vnz15x84	25	25	RNA	RNA	NNP
cord-004738-vnz15x84	25	26	polymerase	polymerase	NN
cord-004738-vnz15x84	25	27	domains	domain	NNS
cord-004738-vnz15x84	25	28	based	base	VBN
cord-004738-vnz15x84	25	29	on	on	IN
cord-004738-vnz15x84	25	30	bioinformatic	bioinformatic	JJ
cord-004738-vnz15x84	25	31	analysis	analysis	NN
cord-004738-vnz15x84	25	32	[	[	-LRB-
cord-004738-vnz15x84	25	33	4	4	CD
cord-004738-vnz15x84	25	34	,	,	,
cord-004738-vnz15x84	25	35	6	6	CD
cord-004738-vnz15x84	25	36	]	]	-RRB-
cord-004738-vnz15x84	25	37	.	.	.
cord-004738-vnz15x84	26	1	The	the	DT
cord-004738-vnz15x84	26	2	first	first	JJ
cord-004738-vnz15x84	26	3	two	two	CD
cord-004738-vnz15x84	26	4	motifs	motif	NNS
cord-004738-vnz15x84	26	5	are	be	VBP
cord-004738-vnz15x84	26	6	located	locate	VBN
cord-004738-vnz15x84	26	7	on	on	IN
cord-004738-vnz15x84	26	8	the	the	DT
cord-004738-vnz15x84	26	9	N	N	NNP
cord-004738-vnz15x84	26	10	and	and	CC
cord-004738-vnz15x84	26	11	C	C	NNP
cord-004738-vnz15x84	26	12	termini	termini	NNPS
cord-004738-vnz15x84	26	13	of	of	IN
cord-004738-vnz15x84	26	14	P150	P150	NNP
cord-004738-vnz15x84	26	15	,	,	,
cord-004738-vnz15x84	26	16	and	and	CC
cord-004738-vnz15x84	26	17	the	the	DT
cord-004738-vnz15x84	26	18	latter	latter	JJ
cord-004738-vnz15x84	26	19	two	two	CD
cord-004738-vnz15x84	26	20	are	be	VBP
cord-004738-vnz15x84	26	21	on	on	IN
cord-004738-vnz15x84	26	22	the	the	DT
cord-004738-vnz15x84	26	23	N	N	NNP
cord-004738-vnz15x84	26	24	and	and	CC
cord-004738-vnz15x84	26	25	C	C	NNP
cord-004738-vnz15x84	26	26	termini	terminus	NNS
cord-004738-vnz15x84	26	27	of	of	IN
cord-004738-vnz15x84	26	28	P90	P90	NNP
cord-004738-vnz15x84	26	29	[	[	-LRB-
cord-004738-vnz15x84	26	30	5	5	CD
cord-004738-vnz15x84	26	31	,	,	,
cord-004738-vnz15x84	26	32	13	13	CD
cord-004738-vnz15x84	26	33	]	]	-RRB-
cord-004738-vnz15x84	26	34	.	.	.
cord-004738-vnz15x84	27	1	Rubella	rubella	NN
cord-004738-vnz15x84	27	2	virus	virus	NN
cord-004738-vnz15x84	27	3	P150-protease	p150-protease	NN
cord-004738-vnz15x84	27	4	was	be	VBD
cord-004738-vnz15x84	27	5	identified	identify	VBN
cord-004738-vnz15x84	27	6	to	to	TO
cord-004738-vnz15x84	27	7	be	be	VB
cord-004738-vnz15x84	27	8	a	a	DT
cord-004738-vnz15x84	27	9	papain	papain	NN
cord-004738-vnz15x84	27	10	-	-	HYPH
cord-004738-vnz15x84	27	11	like	like	IN
cord-004738-vnz15x84	27	12	-	-	HYPH
cord-004738-vnz15x84	27	13	cysteine	cysteine	NN
cord-004738-vnz15x84	27	14	protease	protease	NN
cord-004738-vnz15x84	27	15	[	[	-LRB-
cord-004738-vnz15x84	27	16	5	5	CD
cord-004738-vnz15x84	27	17	]	]	-RRB-
cord-004738-vnz15x84	27	18	.	.	.
cord-004738-vnz15x84	28	1	It	-PRON-	PRP
cord-004738-vnz15x84	28	2	utilizes	utilize	VBZ
cord-004738-vnz15x84	28	3	divalent	divalent	JJ
cord-004738-vnz15x84	28	4	cations	cation	NNS
cord-004738-vnz15x84	28	5	and	and	CC
cord-004738-vnz15x84	28	6	also	also	RB
cord-004738-vnz15x84	28	7	demonstrates	demonstrate	VBZ
cord-004738-vnz15x84	28	8	trans	trans	JJ
cord-004738-vnz15x84	28	9	-	-	JJ
cord-004738-vnz15x84	28	10	cleavage	cleavage	JJ
cord-004738-vnz15x84	28	11	activity	activity	NN
cord-004738-vnz15x84	28	12	on	on	IN
cord-004738-vnz15x84	28	13	homologous	homologous	JJ
cord-004738-vnz15x84	28	14	substrates	substrate	NNS
cord-004738-vnz15x84	28	15	in	in	IN
cord-004738-vnz15x84	28	16	vitro	vitro	FW
cord-004738-vnz15x84	28	17	[	[	-LRB-
cord-004738-vnz15x84	28	18	9	9	CD
cord-004738-vnz15x84	28	19	]	]	-RRB-
cord-004738-vnz15x84	28	20	as	as	RB
cord-004738-vnz15x84	28	21	well	well	RB
cord-004738-vnz15x84	28	22	as	as	IN
cord-004738-vnz15x84	28	23	in	in	IN
cord-004738-vnz15x84	28	24	vivo	vivo	NNP
cord-004738-vnz15x84	28	25	[	[	-LRB-
cord-004738-vnz15x84	28	26	17	17	CD
cord-004738-vnz15x84	28	27	]	]	-RRB-
cord-004738-vnz15x84	28	28	.	.	.
cord-004738-vnz15x84	29	1	Further	further	JJ
cord-004738-vnz15x84	29	2	characterization	characterization	NN
cord-004738-vnz15x84	29	3	of	of	IN
cord-004738-vnz15x84	29	4	RV	RV	NNP
cord-004738-vnz15x84	29	5	protease	protease	NN
cord-004738-vnz15x84	29	6	revealed	reveal	VBD
cord-004738-vnz15x84	29	7	that	that	IN
cord-004738-vnz15x84	29	8	it	-PRON-	PRP
cord-004738-vnz15x84	29	9	exhibits	exhibit	VBZ
cord-004738-vnz15x84	29	10	zinc	zinc	NN
cord-004738-vnz15x84	29	11	-	-	HYPH
cord-004738-vnz15x84	29	12	binding	bind	VBG
cord-004738-vnz15x84	29	13	activity	activity	NN
cord-004738-vnz15x84	29	14	as	as	IN
cord-004738-vnz15x84	29	15	integral	integral	JJ
cord-004738-vnz15x84	29	16	to	to	IN
cord-004738-vnz15x84	29	17	its	-PRON-	PRP$
cord-004738-vnz15x84	29	18	protease	protease	NN
cord-004738-vnz15x84	29	19	activity	activity	NN
cord-004738-vnz15x84	29	20	,	,	,
cord-004738-vnz15x84	29	21	thus	thus	RB
cord-004738-vnz15x84	29	22	suggesting	suggest	VBG
cord-004738-vnz15x84	29	23	that	that	IN
cord-004738-vnz15x84	29	24	it	-PRON-	PRP
cord-004738-vnz15x84	29	25	is	be	VBZ
cord-004738-vnz15x84	29	26	a	a	DT
cord-004738-vnz15x84	29	27	novel	novel	JJ
cord-004738-vnz15x84	29	28	viral	viral	JJ
cord-004738-vnz15x84	29	29	metalloprotease	metalloprotease	NN
cord-004738-vnz15x84	29	30	[	[	-LRB-
cord-004738-vnz15x84	29	31	9	9	CD
cord-004738-vnz15x84	29	32	,	,	,
cord-004738-vnz15x84	29	33	10	10	CD
cord-004738-vnz15x84	29	34	]	]	-RRB-
cord-004738-vnz15x84	29	35	.	.	.
cord-004738-vnz15x84	30	1	Within	within	IN
cord-004738-vnz15x84	30	2	the	the	DT
cord-004738-vnz15x84	30	3	C	C	NNP
cord-004738-vnz15x84	30	4	-	-	HYPH
cord-004738-vnz15x84	30	5	terminal	terminal	NN
cord-004738-vnz15x84	30	6	half	half	NN
cord-004738-vnz15x84	30	7	of	of	IN
cord-004738-vnz15x84	30	8	P150	P150	NNP
cord-004738-vnz15x84	30	9	,	,	,
cord-004738-vnz15x84	30	10	Cys-1152	cys-1152	NN
cord-004738-vnz15x84	30	11	and	and	CC
cord-004738-vnz15x84	30	12	His-1273	his-1273	NN
cord-004738-vnz15x84	30	13	are	be	VBP
cord-004738-vnz15x84	30	14	the	the	DT
cord-004738-vnz15x84	30	15	catalytic	catalytic	JJ
cord-004738-vnz15x84	30	16	sites	site	NNS
cord-004738-vnz15x84	30	17	for	for	IN
cord-004738-vnz15x84	30	18	this	this	DT
cord-004738-vnz15x84	30	19	protease	protease	NN
cord-004738-vnz15x84	30	20	[	[	-LRB-
cord-004738-vnz15x84	30	21	3	3	CD
cord-004738-vnz15x84	30	22	,	,	,
cord-004738-vnz15x84	30	23	11	11	CD
cord-004738-vnz15x84	30	24	]	]	-RRB-
cord-004738-vnz15x84	30	25	.	.	.
cord-004738-vnz15x84	31	1	Domains	domain	NNS
cord-004738-vnz15x84	31	2	of	of	IN
cord-004738-vnz15x84	31	3	RV	RV	NNP
cord-004738-vnz15x84	31	4	protease	protease	NN
cord-004738-vnz15x84	31	5	required	require	VBN
cord-004738-vnz15x84	31	6	for	for	IN
cord-004738-vnz15x84	31	7	cis	cis	NNP
cord-004738-vnz15x84	31	8	-	-	HYPH
cord-004738-vnz15x84	31	9	and	and	CC
cord-004738-vnz15x84	31	10	trans	trans	NN
cord-004738-vnz15x84	31	11	-	-	NN
cord-004738-vnz15x84	31	12	activity	activity	NN
cord-004738-vnz15x84	31	13	are	be	VBP
cord-004738-vnz15x84	31	14	mapped	map	VBN
cord-004738-vnz15x84	31	15	[	[	-LRB-
cord-004738-vnz15x84	31	16	8	8	CD
cord-004738-vnz15x84	31	17	,	,	,
cord-004738-vnz15x84	31	18	11	11	CD
cord-004738-vnz15x84	31	19	]	]	-RRB-
cord-004738-vnz15x84	31	20	.	.	.
cord-004738-vnz15x84	32	1	These	these	DT
cord-004738-vnz15x84	32	2	studies	study	NNS
cord-004738-vnz15x84	32	3	have	have	VBP
cord-004738-vnz15x84	32	4	thus	thus	RB
cord-004738-vnz15x84	32	5	far	far	RB
cord-004738-vnz15x84	32	6	focused	focused	JJ
cord-004738-vnz15x84	32	7	on	on	IN
cord-004738-vnz15x84	32	8	defining	define	VBG
cord-004738-vnz15x84	32	9	the	the	DT
cord-004738-vnz15x84	32	10	catalytic	catalytic	JJ
cord-004738-vnz15x84	32	11	sites	site	NNS
cord-004738-vnz15x84	32	12	,	,	,
cord-004738-vnz15x84	32	13	active	active	JJ
cord-004738-vnz15x84	32	14	domains	domain	NNS
cord-004738-vnz15x84	32	15	,	,	,
cord-004738-vnz15x84	32	16	and	and	CC
cord-004738-vnz15x84	32	17	cis	cis	NNP
cord-004738-vnz15x84	32	18	-	-	HYPH
cord-004738-vnz15x84	32	19	and	and	CC
cord-004738-vnz15x84	32	20	trans	trans	JJ
cord-004738-vnz15x84	32	21	-	-	NN
cord-004738-vnz15x84	32	22	activity	activity	NN
cord-004738-vnz15x84	32	23	requirements	requirement	NNS
cord-004738-vnz15x84	32	24	of	of	IN
cord-004738-vnz15x84	32	25	RV	rv	NN
cord-004738-vnz15x84	32	26	protease	protease	NN
cord-004738-vnz15x84	32	27	[	[	-LRB-
cord-004738-vnz15x84	32	28	8	8	CD
cord-004738-vnz15x84	32	29	]	]	-RRB-
cord-004738-vnz15x84	33	1	[	[	-LRB-
cord-004738-vnz15x84	33	2	9	9	CD
cord-004738-vnz15x84	33	3	]	]	-RRB-
cord-004738-vnz15x84	33	4	[	[	-LRB-
cord-004738-vnz15x84	33	5	10	10	CD
cord-004738-vnz15x84	33	6	]	]	-RRB-
cord-004738-vnz15x84	34	1	[	[	-LRB-
cord-004738-vnz15x84	34	2	11	11	CD
cord-004738-vnz15x84	34	3	]	]	-RRB-
cord-004738-vnz15x84	34	4	17	17	CD
cord-004738-vnz15x84	34	5	]	]	-RRB-
cord-004738-vnz15x84	34	6	.	.	.
cord-004738-vnz15x84	35	1	Experimental	experimental	JJ
cord-004738-vnz15x84	35	2	evidence	evidence	NN
cord-004738-vnz15x84	35	3	suggests	suggest	VBZ
cord-004738-vnz15x84	35	4	that	that	IN
cord-004738-vnz15x84	35	5	P200	p200	NN
cord-004738-vnz15x84	35	6	is	be	VBZ
cord-004738-vnz15x84	35	7	actively	actively	RB
cord-004738-vnz15x84	35	8	involved	involve	VBN
cord-004738-vnz15x84	35	9	in	in	IN
cord-004738-vnz15x84	35	10	the	the	DT
cord-004738-vnz15x84	35	11	synthesis	synthesis	NN
cord-004738-vnz15x84	35	12	of	of	IN
cord-004738-vnz15x84	35	13	viral	viral	JJ
cord-004738-vnz15x84	35	14	negative	negative	JJ
cord-004738-vnz15x84	35	15	-	-	HYPH
cord-004738-vnz15x84	35	16	strand	strand	NN
cord-004738-vnz15x84	35	17	RNA	RNA	NNP
cord-004738-vnz15x84	35	18	,	,	,
cord-004738-vnz15x84	35	19	and	and	CC
cord-004738-vnz15x84	35	20	its	-PRON-	PRP$
cord-004738-vnz15x84	35	21	cleavage	cleavage	NN
cord-004738-vnz15x84	35	22	into	into	IN
cord-004738-vnz15x84	35	23	P150	P150	NNP
cord-004738-vnz15x84	35	24	and	and	CC
cord-004738-vnz15x84	35	25	P90	P90	NNP
cord-004738-vnz15x84	35	26	has	have	VBZ
cord-004738-vnz15x84	35	27	been	be	VBN
cord-004738-vnz15x84	35	28	suggested	suggest	VBN
cord-004738-vnz15x84	35	29	to	to	TO
cord-004738-vnz15x84	35	30	switch	switch	VB
cord-004738-vnz15x84	35	31	the	the	DT
cord-004738-vnz15x84	35	32	complex	complex	NN
cord-004738-vnz15x84	35	33	to	to	TO
cord-004738-vnz15x84	35	34	initiate	initiate	VB
cord-004738-vnz15x84	35	35	positive	positive	JJ
cord-004738-vnz15x84	35	36	RNA	RNA	NNP
cord-004738-vnz15x84	35	37	synthesis	synthesis	NN
cord-004738-vnz15x84	35	38	[	[	-LRB-
cord-004738-vnz15x84	35	39	8	8	CD
cord-004738-vnz15x84	35	40	]	]	-RRB-
cord-004738-vnz15x84	35	41	.	.	.
cord-004738-vnz15x84	36	1	This	this	DT
cord-004738-vnz15x84	36	2	leads	lead	VBZ
cord-004738-vnz15x84	36	3	to	to	IN
cord-004738-vnz15x84	36	4	the	the	DT
cord-004738-vnz15x84	36	5	logical	logical	JJ
cord-004738-vnz15x84	36	6	hypothesis	hypothesis	NN
cord-004738-vnz15x84	36	7	that	that	IN
cord-004738-vnz15x84	36	8	,	,	,
cord-004738-vnz15x84	36	9	in	in	IN
cord-004738-vnz15x84	36	10	the	the	DT
cord-004738-vnz15x84	36	11	replication	replication	NN
cord-004738-vnz15x84	36	12	complex	complex	NN
cord-004738-vnz15x84	36	13	,	,	,
cord-004738-vnz15x84	36	14	those	those	DT
cord-004738-vnz15x84	36	15	P200	p200	NN
cord-004738-vnz15x84	36	16	molecules	molecule	NNS
cord-004738-vnz15x84	36	17	engaged	engage	VBN
cord-004738-vnz15x84	36	18	in	in	IN
cord-004738-vnz15x84	36	19	negative	negative	JJ
cord-004738-vnz15x84	36	20	-	-	HYPH
cord-004738-vnz15x84	36	21	strand	strand	NN
cord-004738-vnz15x84	36	22	RNA	RNA	NNP
cord-004738-vnz15x84	36	23	synthesis	synthesis	NN
cord-004738-vnz15x84	36	24	must	must	MD
cord-004738-vnz15x84	36	25	remain	remain	VB
cord-004738-vnz15x84	36	26	as	as	IN
cord-004738-vnz15x84	36	27	P200	P200	NNP
cord-004738-vnz15x84	36	28	(	(	-LRB-
cord-004738-vnz15x84	36	29	i.e.	i.e.	FW
cord-004738-vnz15x84	36	30	,	,	,
cord-004738-vnz15x84	36	31	lose	lose	VB
cord-004738-vnz15x84	36	32	their	-PRON-	PRP$
cord-004738-vnz15x84	36	33	cis	cis	JJ
cord-004738-vnz15x84	36	34	-	-	HYPH
cord-004738-vnz15x84	36	35	protease	protease	JJ
cord-004738-vnz15x84	36	36	activity	activity	NN
cord-004738-vnz15x84	36	37	)	)	-RRB-
cord-004738-vnz15x84	36	38	as	as	RB
cord-004738-vnz15x84	36	39	long	long	RB
cord-004738-vnz15x84	36	40	as	as	IN
cord-004738-vnz15x84	36	41	they	-PRON-	PRP
cord-004738-vnz15x84	36	42	are	be	VBP
cord-004738-vnz15x84	36	43	required	require	VBN
cord-004738-vnz15x84	36	44	to	to	TO
cord-004738-vnz15x84	36	45	participate	participate	VB
cord-004738-vnz15x84	36	46	in	in	IN
cord-004738-vnz15x84	36	47	negativestrand	negativestrand	NNP
cord-004738-vnz15x84	36	48	RNA	RNA	NNP
cord-004738-vnz15x84	36	49	synthesis	synthesis	NN
cord-004738-vnz15x84	36	50	.	.	.
cord-004738-vnz15x84	37	1	Subsequently	subsequently	RB
cord-004738-vnz15x84	37	2	,	,	,
cord-004738-vnz15x84	37	3	the	the	DT
cord-004738-vnz15x84	37	4	P150-protease	p150-protease	JJ
cord-004738-vnz15x84	37	5	trans	trans	NN
cord-004738-vnz15x84	37	6	-	-	NN
cord-004738-vnz15x84	37	7	activity	activity	NN
cord-004738-vnz15x84	37	8	cleaves	cleave	VBZ
cord-004738-vnz15x84	37	9	these	these	DT
cord-004738-vnz15x84	37	10	P200	p200	NN
cord-004738-vnz15x84	37	11	molecules	molecule	NNS
cord-004738-vnz15x84	37	12	to	to	TO
cord-004738-vnz15x84	37	13	signal	signal	VB
cord-004738-vnz15x84	37	14	the	the	DT
cord-004738-vnz15x84	37	15	replication	replication	NN
cord-004738-vnz15x84	37	16	complex	complex	NN
cord-004738-vnz15x84	37	17	to	to	TO
cord-004738-vnz15x84	37	18	switch	switch	VB
cord-004738-vnz15x84	37	19	to	to	IN
cord-004738-vnz15x84	37	20	positivestrand	positivestrand	NNP
cord-004738-vnz15x84	37	21	RNA	RNA	NNP
cord-004738-vnz15x84	37	22	synthesis	synthesis	NN
cord-004738-vnz15x84	37	23	mode	mode	NN
cord-004738-vnz15x84	37	24	.	.	.
cord-004738-vnz15x84	38	1	However	however	RB
cord-004738-vnz15x84	38	2	,	,	,
cord-004738-vnz15x84	38	3	besides	besides	IN
cord-004738-vnz15x84	38	4	the	the	DT
cord-004738-vnz15x84	38	5	presence	presence	NN
cord-004738-vnz15x84	38	6	of	of	IN
cord-004738-vnz15x84	38	7	the	the	DT
cord-004738-vnz15x84	38	8	cleavage	cleavage	NN
cord-004738-vnz15x84	38	9	site	site	NN
cord-004738-vnz15x84	38	10	,	,	,
cord-004738-vnz15x84	38	11	what	what	WP
cord-004738-vnz15x84	38	12	the	the	DT
cord-004738-vnz15x84	38	13	internal	internal	JJ
cord-004738-vnz15x84	38	14	sequence	sequence	NN
cord-004738-vnz15x84	38	15	requirements	requirement	NNS
cord-004738-vnz15x84	38	16	of	of	IN
cord-004738-vnz15x84	38	17	the	the	DT
cord-004738-vnz15x84	38	18	P200	p200	NN
cord-004738-vnz15x84	38	19	and	and	CC
cord-004738-vnz15x84	38	20	its	-PRON-	PRP$
cord-004738-vnz15x84	38	21	intermediaries	intermediary	NNS
cord-004738-vnz15x84	38	22	for	for	IN
cord-004738-vnz15x84	38	23	RV	rv	NN
cord-004738-vnz15x84	38	24	protease	protease	NN
cord-004738-vnz15x84	38	25	trans	trans	JJ
cord-004738-vnz15x84	38	26	-	-	JJ
cord-004738-vnz15x84	38	27	cleavage	cleavage	JJ
cord-004738-vnz15x84	38	28	activity	activity	NN
cord-004738-vnz15x84	38	29	are	be	VBP
cord-004738-vnz15x84	38	30	,	,	,
cord-004738-vnz15x84	38	31	and	and	CC
cord-004738-vnz15x84	38	32	how	how	WRB
cord-004738-vnz15x84	38	33	the	the	DT
cord-004738-vnz15x84	38	34	cis	cis	NN
cord-004738-vnz15x84	38	35	-	-	HYPH
cord-004738-vnz15x84	38	36	activity	activity	NN
cord-004738-vnz15x84	38	37	of	of	IN
cord-004738-vnz15x84	38	38	P200	P200	NNP
cord-004738-vnz15x84	38	39	is	be	VBZ
cord-004738-vnz15x84	38	40	regulated	regulate	VBN
cord-004738-vnz15x84	38	41	to	to	TO
cord-004738-vnz15x84	38	42	maintain	maintain	VB
cord-004738-vnz15x84	38	43	optimal	optimal	JJ
cord-004738-vnz15x84	38	44	negative	negative	JJ
cord-004738-vnz15x84	38	45	RNA	RNA	NNP
cord-004738-vnz15x84	38	46	synthesis	synthesis	NN
cord-004738-vnz15x84	38	47	have	have	VBP
cord-004738-vnz15x84	38	48	not	not	RB
cord-004738-vnz15x84	38	49	been	be	VBN
cord-004738-vnz15x84	38	50	identified	identify	VBN
cord-004738-vnz15x84	38	51	.	.	.
cord-004738-vnz15x84	39	1	In	in	IN
cord-004738-vnz15x84	39	2	this	this	DT
cord-004738-vnz15x84	39	3	report	report	NN
cord-004738-vnz15x84	39	4	,	,	,
cord-004738-vnz15x84	39	5	we	-PRON-	PRP
cord-004738-vnz15x84	39	6	analyzed	analyze	VBD
cord-004738-vnz15x84	39	7	the	the	DT
cord-004738-vnz15x84	39	8	substrate	substrate	NN
cord-004738-vnz15x84	39	9	features	feature	NNS
cord-004738-vnz15x84	39	10	that	that	WDT
cord-004738-vnz15x84	39	11	are	be	VBP
cord-004738-vnz15x84	39	12	required	require	VBN
cord-004738-vnz15x84	39	13	of	of	IN
cord-004738-vnz15x84	39	14	the	the	DT
cord-004738-vnz15x84	39	15	RV	RV	NNP
cord-004738-vnz15x84	39	16	protease	protease	NN
cord-004738-vnz15x84	39	17	trans	trans	NN
cord-004738-vnz15x84	39	18	-	-	NN
cord-004738-vnz15x84	39	19	activity	activity	NN
cord-004738-vnz15x84	39	20	by	by	IN
cord-004738-vnz15x84	39	21	using	use	VBG
cord-004738-vnz15x84	39	22	a	a	DT
cord-004738-vnz15x84	39	23	truncated	truncated	JJ
cord-004738-vnz15x84	39	24	version	version	NN
cord-004738-vnz15x84	39	25	of	of	IN
cord-004738-vnz15x84	39	26	RV	RV	NNP
cord-004738-vnz15x84	39	27	P200	P200	NNP
cord-004738-vnz15x84	39	28	(	(	-LRB-
cord-004738-vnz15x84	39	29	amino	amino	NN
cord-004738-vnz15x84	39	30	acid	acid	NN
cord-004738-vnz15x84	39	31	residues	residue	NNS
cord-004738-vnz15x84	39	32	827	827	CD
cord-004738-vnz15x84	39	33	-	-	HYPH
cord-004738-vnz15x84	39	34	1548	1548	CD
cord-004738-vnz15x84	39	35	)	)	-RRB-
cord-004738-vnz15x84	39	36	that	that	WDT
cord-004738-vnz15x84	39	37	was	be	VBD
cord-004738-vnz15x84	39	38	previously	previously	RB
cord-004738-vnz15x84	39	39	shown	show	VBN
cord-004738-vnz15x84	39	40	to	to	TO
cord-004738-vnz15x84	39	41	function	function	VB
cord-004738-vnz15x84	39	42	as	as	IN
cord-004738-vnz15x84	39	43	protease	protease	NN
cord-004738-vnz15x84	39	44	but	but	CC
cord-004738-vnz15x84	39	45	had	have	VBD
cord-004738-vnz15x84	39	46	lost	lose	VBN
cord-004738-vnz15x84	39	47	its	-PRON-	PRP$
cord-004738-vnz15x84	39	48	cis	cis	JJ
cord-004738-vnz15x84	39	49	-	-	HYPH
cord-004738-vnz15x84	39	50	activity	activity	NN
cord-004738-vnz15x84	39	51	due	due	IN
cord-004738-vnz15x84	39	52	to	to	IN
cord-004738-vnz15x84	39	53	a	a	DT
cord-004738-vnz15x84	39	54	substitution	substitution	NN
cord-004738-vnz15x84	39	55	,	,	,
cord-004738-vnz15x84	39	56	G1301S	G1301S	NNP
cord-004738-vnz15x84	39	57	,	,	,
cord-004738-vnz15x84	39	58	in	in	IN
cord-004738-vnz15x84	39	59	the	the	DT
cord-004738-vnz15x84	39	60	cleavage	cleavage	NN
cord-004738-vnz15x84	39	61	site	site	NN
cord-004738-vnz15x84	39	62	,	,	,
cord-004738-vnz15x84	39	63	and	and	CC
cord-004738-vnz15x84	39	64	a	a	DT
cord-004738-vnz15x84	39	65	series	series	NN
cord-004738-vnz15x84	39	66	of	of	IN
cord-004738-vnz15x84	39	67	P200-derived	p200-derived	JJ
cord-004738-vnz15x84	39	68	substrates	substrate	NNS
cord-004738-vnz15x84	39	69	that	that	WDT
cord-004738-vnz15x84	39	70	lack	lack	VBP
cord-004738-vnz15x84	39	71	protease	protease	NN
cord-004738-vnz15x84	39	72	activity	activity	NN
cord-004738-vnz15x84	39	73	due	due	IN
cord-004738-vnz15x84	39	74	to	to	IN
cord-004738-vnz15x84	39	75	an	an	DT
cord-004738-vnz15x84	39	76	amino	amino	NN
cord-004738-vnz15x84	39	77	acid	acid	NN
cord-004738-vnz15x84	39	78	substitution	substitution	NN
cord-004738-vnz15x84	39	79	,	,	,
cord-004738-vnz15x84	39	80	C1152S	C1152S	NNP
cord-004738-vnz15x84	39	81	,	,	,
cord-004738-vnz15x84	39	82	in	in	IN
cord-004738-vnz15x84	39	83	the	the	DT
cord-004738-vnz15x84	39	84	catalytic	catalytic	JJ
cord-004738-vnz15x84	39	85	dyad	dyad	NN
cord-004738-vnz15x84	39	86	[	[	-LRB-
cord-004738-vnz15x84	39	87	8	8	CD
cord-004738-vnz15x84	39	88	]	]	-RRB-
cord-004738-vnz15x84	39	89	.	.	.
cord-004738-vnz15x84	40	1	Our	-PRON-	PRP$
cord-004738-vnz15x84	40	2	analysis	analysis	NN
cord-004738-vnz15x84	40	3	identified	identify	VBD
cord-004738-vnz15x84	40	4	a	a	DT
cord-004738-vnz15x84	40	5	region	region	NN
cord-004738-vnz15x84	40	6	in	in	IN
cord-004738-vnz15x84	40	7	the	the	DT
cord-004738-vnz15x84	40	8	substrate	substrate	NN
cord-004738-vnz15x84	40	9	corresponding	correspond	VBG
cord-004738-vnz15x84	40	10	to	to	IN
cord-004738-vnz15x84	40	11	residues	residue	NNS
cord-004738-vnz15x84	40	12	994	994	CD
cord-004738-vnz15x84	40	13	-	-	HYPH
cord-004738-vnz15x84	40	14	1102	1102	CD
cord-004738-vnz15x84	40	15	of	of	IN
cord-004738-vnz15x84	40	16	the	the	DT
cord-004738-vnz15x84	40	17	P200	P200	NNP
cord-004738-vnz15x84	40	18	polyprotein	polyprotein	NN
cord-004738-vnz15x84	40	19	that	that	WDT
cord-004738-vnz15x84	40	20	is	be	VBZ
cord-004738-vnz15x84	40	21	required	require	VBN
cord-004738-vnz15x84	40	22	for	for	IN
cord-004738-vnz15x84	40	23	the	the	DT
cord-004738-vnz15x84	40	24	protease	protease	NN
cord-004738-vnz15x84	40	25	trans	trans	NN
cord-004738-vnz15x84	40	26	-	-	NN
cord-004738-vnz15x84	40	27	activity	activity	NN
cord-004738-vnz15x84	40	28	,	,	,
cord-004738-vnz15x84	40	29	and	and	CC
cord-004738-vnz15x84	40	30	this	this	DT
cord-004738-vnz15x84	40	31	region	region	NN
cord-004738-vnz15x84	40	32	overlaps	overlap	VBZ
cord-004738-vnz15x84	40	33	with	with	IN
cord-004738-vnz15x84	40	34	the	the	DT
cord-004738-vnz15x84	40	35	starting	starting	NN
cord-004738-vnz15x84	40	36	site	site	NN
cord-004738-vnz15x84	40	37	of	of	IN
cord-004738-vnz15x84	40	38	the	the	DT
cord-004738-vnz15x84	40	39	essential	essential	JJ
cord-004738-vnz15x84	40	40	cis	cis	JJ
cord-004738-vnz15x84	40	41	-	-	HYPH
cord-004738-vnz15x84	40	42	protease	protease	JJ
cord-004738-vnz15x84	40	43	activity	activity	NN
cord-004738-vnz15x84	40	44	of	of	IN
cord-004738-vnz15x84	40	45	RV	RV	NNP
cord-004738-vnz15x84	40	46	P200	P200	NNP
cord-004738-vnz15x84	40	47	polyprotein	polyprotein	NN
cord-004738-vnz15x84	40	48	.	.	.
cord-004738-vnz15x84	41	1	Potential	potential	JJ
cord-004738-vnz15x84	41	2	implications	implication	NNS
cord-004738-vnz15x84	41	3	of	of	IN
cord-004738-vnz15x84	41	4	the	the	DT
cord-004738-vnz15x84	41	5	requirement	requirement	NN
cord-004738-vnz15x84	41	6	of	of	IN
cord-004738-vnz15x84	41	7	the	the	DT
cord-004738-vnz15x84	41	8	cis	cis	JJ
cord-004738-vnz15x84	41	9	-	-	HYPH
cord-004738-vnz15x84	41	10	activity	activity	NN
cord-004738-vnz15x84	41	11	region	region	NN
cord-004738-vnz15x84	41	12	of	of	IN
cord-004738-vnz15x84	41	13	P200	p200	NN
cord-004738-vnz15x84	41	14	substrates	substrate	NNS
cord-004738-vnz15x84	41	15	for	for	IN
cord-004738-vnz15x84	41	16	the	the	DT
cord-004738-vnz15x84	41	17	RV	RV	NNP
cord-004738-vnz15x84	41	18	protease	protease	NN
cord-004738-vnz15x84	41	19	trans	trans	NN
cord-004738-vnz15x84	41	20	-	-	NN
cord-004738-vnz15x84	41	21	activity	activity	NN
cord-004738-vnz15x84	41	22	are	be	VBP
cord-004738-vnz15x84	41	23	discussed	discuss	VBN
cord-004738-vnz15x84	41	24	.	.	.
cord-004738-vnz15x84	42	1	Plasmids	plasmid	NNS
cord-004738-vnz15x84	42	2	capable	capable	JJ
cord-004738-vnz15x84	42	3	of	of	IN
cord-004738-vnz15x84	42	4	expressing	express	VBG
cord-004738-vnz15x84	42	5	functional	functional	JJ
cord-004738-vnz15x84	42	6	RV	RV	NNP
cord-004738-vnz15x84	42	7	protease	protease	NN
cord-004738-vnz15x84	42	8	and	and	CC
cord-004738-vnz15x84	42	9	P200-derived	p200-derived	JJ
cord-004738-vnz15x84	42	10	substrates	substrate	NNS
cord-004738-vnz15x84	42	11	in	in	IN
cord-004738-vnz15x84	42	12	mammalian	mammalian	JJ
cord-004738-vnz15x84	42	13	cells	cell	NNS
cord-004738-vnz15x84	42	14	were	be	VBD
cord-004738-vnz15x84	42	15	constructed	construct	VBN
cord-004738-vnz15x84	42	16	as	as	IN
cord-004738-vnz15x84	42	17	follows	follow	VBZ
cord-004738-vnz15x84	42	18	:	:	:
cord-004738-vnz15x84	43	1	Appropriate	appropriate	JJ
cord-004738-vnz15x84	43	2	cDNA	cdna	NN
cord-004738-vnz15x84	43	3	segments	segment	NNS
cord-004738-vnz15x84	43	4	representing	represent	VBG
cord-004738-vnz15x84	43	5	various	various	JJ
cord-004738-vnz15x84	43	6	RV	RV	NNP
cord-004738-vnz15x84	43	7	genomic	genomic	JJ
cord-004738-vnz15x84	43	8	regions	region	NNS
cord-004738-vnz15x84	43	9	were	be	VBD
cord-004738-vnz15x84	43	10	PCR	PCR	NNP
cord-004738-vnz15x84	43	11	-	-	HYPH
cord-004738-vnz15x84	43	12	amplified	amplify	VBN
cord-004738-vnz15x84	43	13	and	and	CC
cord-004738-vnz15x84	43	14	inserted	insert	VBN
cord-004738-vnz15x84	43	15	into	into	IN
cord-004738-vnz15x84	43	16	a	a	DT
cord-004738-vnz15x84	43	17	mammalian	mammalian	JJ
cord-004738-vnz15x84	43	18	expression	expression	NN
cord-004738-vnz15x84	43	19	plasmid	plasmid	NN
cord-004738-vnz15x84	43	20	,	,	,
cord-004738-vnz15x84	43	21	pcDNA4-His	pcDNA4-His	NNP
cord-004738-vnz15x84	43	22	/	/	SYM
cord-004738-vnz15x84	43	23	Max	Max	NNP
cord-004738-vnz15x84	43	24	-	-	HYPH
cord-004738-vnz15x84	43	25	C	C	NNP
cord-004738-vnz15x84	43	26	,	,	,
cord-004738-vnz15x84	43	27	driven	drive	VBN
cord-004738-vnz15x84	43	28	by	by	IN
cord-004738-vnz15x84	43	29	a	a	DT
cord-004738-vnz15x84	43	30	CMV	CMV	NNP
cord-004738-vnz15x84	43	31	promoter	promoter	NN
cord-004738-vnz15x84	43	32	.	.	.
cord-004738-vnz15x84	44	1	This	this	DT
cord-004738-vnz15x84	44	2	plasmid	plasmid	NN
cord-004738-vnz15x84	44	3	also	also	RB
cord-004738-vnz15x84	44	4	contains	contain	VBZ
cord-004738-vnz15x84	44	5	an	an	DT
cord-004738-vnz15x84	44	6	in	in	IN
cord-004738-vnz15x84	44	7	-	-	HYPH
cord-004738-vnz15x84	44	8	frame	frame	NN
cord-004738-vnz15x84	44	9	Xpress	Xpress	NNP
cord-004738-vnz15x84	44	10	epitope	epitope	NN
cord-004738-vnz15x84	44	11	tag	tag	NN
cord-004738-vnz15x84	44	12	for	for	IN
cord-004738-vnz15x84	44	13	easy	easy	JJ
cord-004738-vnz15x84	44	14	identification	identification	NN
cord-004738-vnz15x84	44	15	of	of	IN
cord-004738-vnz15x84	44	16	the	the	DT
cord-004738-vnz15x84	44	17	expressed	express	VBN
cord-004738-vnz15x84	44	18	protein	protein	NN
cord-004738-vnz15x84	44	19	by	by	IN
cord-004738-vnz15x84	44	20	anti	anti	JJ
cord-004738-vnz15x84	44	21	-	-	JJ
cord-004738-vnz15x84	44	22	Xpress	xpress	JJ
cord-004738-vnz15x84	44	23	monoclonal	monoclonal	JJ
cord-004738-vnz15x84	44	24	antibody	antibody	NN
cord-004738-vnz15x84	44	25	in	in	IN
cord-004738-vnz15x84	44	26	immunobloting	immunoblote	VBG
cord-004738-vnz15x84	44	27	analysis	analysis	NN
cord-004738-vnz15x84	44	28	(	(	-LRB-
cord-004738-vnz15x84	44	29	Invitrogen	Invitrogen	NNP
cord-004738-vnz15x84	44	30	Inc	Inc	NNP
cord-004738-vnz15x84	44	31	,	,	,
cord-004738-vnz15x84	44	32	CA	CA	NNP
cord-004738-vnz15x84	44	33	)	)	-RRB-
cord-004738-vnz15x84	44	34	.	.	.
cord-004738-vnz15x84	45	1	Two	two	CD
cord-004738-vnz15x84	45	2	independent	independent	JJ
cord-004738-vnz15x84	45	3	DNA	dna	NN
cord-004738-vnz15x84	45	4	preparations	preparation	NNS
cord-004738-vnz15x84	45	5	for	for	IN
cord-004738-vnz15x84	45	6	each	each	DT
cord-004738-vnz15x84	45	7	plasmid	plasmid	NN
cord-004738-vnz15x84	45	8	were	be	VBD
cord-004738-vnz15x84	45	9	sequenced	sequence	VBN
cord-004738-vnz15x84	45	10	to	to	TO
cord-004738-vnz15x84	45	11	ensure	ensure	VB
cord-004738-vnz15x84	45	12	authenticity	authenticity	NN
cord-004738-vnz15x84	45	13	of	of	IN
cord-004738-vnz15x84	45	14	the	the	DT
cord-004738-vnz15x84	45	15	plasmids	plasmid	NNS
cord-004738-vnz15x84	45	16	used	use	VBN
cord-004738-vnz15x84	45	17	in	in	IN
cord-004738-vnz15x84	45	18	the	the	DT
cord-004738-vnz15x84	45	19	study	study	NN
cord-004738-vnz15x84	45	20	.	.	.
cord-004738-vnz15x84	46	1	RV	RV	NNP
cord-004738-vnz15x84	46	2	trans	trans	JJ
cord-004738-vnz15x84	46	3	-	-	NN
cord-004738-vnz15x84	46	4	cleavage	cleavage	JJ
cord-004738-vnz15x84	46	5	active	active	JJ
cord-004738-vnz15x84	46	6	protease	protease	NN
cord-004738-vnz15x84	46	7	-	-	HYPH
cord-004738-vnz15x84	46	8	encoding	encode	VBG
cord-004738-vnz15x84	46	9	cDNA	cdna	NN
cord-004738-vnz15x84	46	10	was	be	VBD
cord-004738-vnz15x84	46	11	amplified	amplify	VBN
cord-004738-vnz15x84	46	12	by	by	IN
cord-004738-vnz15x84	46	13	PCR	PCR	NNP
cord-004738-vnz15x84	46	14	with	with	IN
cord-004738-vnz15x84	46	15	a	a	DT
cord-004738-vnz15x84	46	16	pair	pair	NN
cord-004738-vnz15x84	46	17	of	of	IN
cord-004738-vnz15x84	46	18	primers	primer	NNS
cord-004738-vnz15x84	46	19	(	(	-LRB-
cord-004738-vnz15x84	46	20	Table	Table	NNP
cord-004738-vnz15x84	46	21	1	1	CD
cord-004738-vnz15x84	46	22	)	)	-RRB-
cord-004738-vnz15x84	46	23	,	,	,
cord-004738-vnz15x84	46	24	using	use	VBG
cord-004738-vnz15x84	46	25	a	a	DT
cord-004738-vnz15x84	46	26	previously	previously	RB
cord-004738-vnz15x84	46	27	well	well	RB
cord-004738-vnz15x84	46	28	-	-	HYPH
cord-004738-vnz15x84	46	29	established	establish	VBN
cord-004738-vnz15x84	46	30	infectious	infectious	JJ
cord-004738-vnz15x84	46	31	RV	RV	NNP
cord-004738-vnz15x84	46	32	cDNA	cdna	NN
cord-004738-vnz15x84	46	33	template	template	NN
cord-004738-vnz15x84	46	34	,	,	,
cord-004738-vnz15x84	46	35	pBRM33-G1301S	pbrm33-g1301s	NN
cord-004738-vnz15x84	46	36	,	,	,
cord-004738-vnz15x84	46	37	which	which	WDT
cord-004738-vnz15x84	46	38	contains	contain	VBZ
cord-004738-vnz15x84	46	39	an	an	DT
cord-004738-vnz15x84	46	40	active	active	JJ
cord-004738-vnz15x84	46	41	protease	protease	NN
cord-004738-vnz15x84	46	42	domain	domain	NN
cord-004738-vnz15x84	46	43	(	(	-LRB-
cord-004738-vnz15x84	46	44	catalytic	catalytic	JJ
cord-004738-vnz15x84	46	45	site	site	NN
cord-004738-vnz15x84	46	46	,	,	,
cord-004738-vnz15x84	46	47	C1152	C1152	NNP
cord-004738-vnz15x84	46	48	)	)	-RRB-
cord-004738-vnz15x84	46	49	but	but	CC
cord-004738-vnz15x84	46	50	lacks	lack	VBZ
cord-004738-vnz15x84	46	51	the	the	DT
cord-004738-vnz15x84	46	52	cis	cis	JJ
cord-004738-vnz15x84	46	53	-	-	HYPH
cord-004738-vnz15x84	46	54	cleavage	cleavage	NN
cord-004738-vnz15x84	46	55	capability	capability	NN
cord-004738-vnz15x84	46	56	due	due	IN
cord-004738-vnz15x84	46	57	to	to	IN
cord-004738-vnz15x84	46	58	a	a	DT
cord-004738-vnz15x84	46	59	substitution	substitution	NN
cord-004738-vnz15x84	46	60	at	at	IN
cord-004738-vnz15x84	46	61	residue	residue	NN
cord-004738-vnz15x84	46	62	G1301	G1301	NNP
cord-004738-vnz15x84	46	63	within	within	IN
cord-004738-vnz15x84	46	64	the	the	DT
cord-004738-vnz15x84	46	65	cleavage	cleavage	NN
cord-004738-vnz15x84	46	66	site	site	NN
cord-004738-vnz15x84	46	67	(	(	-LRB-
cord-004738-vnz15x84	46	68	NH	NH	NNP
cord-004738-vnz15x84	46	69	2	2	CD
cord-004738-vnz15x84	46	70	-SRGG	-SRGG	NNP
cord-004738-vnz15x84	46	71	/	/	SYM
cord-004738-vnz15x84	46	72	G	g	NN
cord-004738-vnz15x84	46	73	-	-	HYPH
cord-004738-vnz15x84	46	74	COOH	COOH	NNP
cord-004738-vnz15x84	46	75	)	)	-RRB-
cord-004738-vnz15x84	46	76	to	to	IN
cord-004738-vnz15x84	46	77	S1301	s1301	NN
cord-004738-vnz15x84	47	1	(	(	-LRB-
cord-004738-vnz15x84	47	2	NH	NH	NNP
cord-004738-vnz15x84	47	3	2	2	CD
cord-004738-vnz15x84	47	4	-SRGS	-SRGS	,
cord-004738-vnz15x84	47	5	/	/	SYM
cord-004738-vnz15x84	47	6	G	g	NN
cord-004738-vnz15x84	47	7	-	-	HYPH
cord-004738-vnz15x84	47	8	COOH	COOH	NNP
cord-004738-vnz15x84	47	9	)	)	-RRB-
cord-004738-vnz15x84	48	1	[	[	-LRB-
cord-004738-vnz15x84	48	2	8	8	CD
cord-004738-vnz15x84	48	3	]	]	-RRB-
cord-004738-vnz15x84	48	4	.	.	.
cord-004738-vnz15x84	49	1	The	the	DT
cord-004738-vnz15x84	49	2	amplified	amplify	VBN
cord-004738-vnz15x84	49	3	DNA	dna	NN
cord-004738-vnz15x84	49	4	product	product	NN
cord-004738-vnz15x84	49	5	was	be	VBD
cord-004738-vnz15x84	49	6	cloned	clone	VBN
cord-004738-vnz15x84	49	7	at	at	IN
cord-004738-vnz15x84	49	8	the	the	DT
cord-004738-vnz15x84	49	9	BamHI	bamhi	NN
cord-004738-vnz15x84	49	10	and	and	CC
cord-004738-vnz15x84	49	11	EcoRI	ecori	NN
cord-004738-vnz15x84	49	12	sites	site	NNS
cord-004738-vnz15x84	49	13	in	in	IN
cord-004738-vnz15x84	49	14	the	the	DT
cord-004738-vnz15x84	49	15	expression	expression	NN
cord-004738-vnz15x84	49	16	plasmid	plasmid	NN
cord-004738-vnz15x84	49	17	described	describe	VBN
cord-004738-vnz15x84	49	18	above	above	RB
cord-004738-vnz15x84	49	19	.	.	.
cord-004738-vnz15x84	50	1	In	in	IN
cord-004738-vnz15x84	50	2	this	this	DT
cord-004738-vnz15x84	50	3	construct	construct	NN
cord-004738-vnz15x84	50	4	,	,	,
cord-004738-vnz15x84	50	5	in	in	IN
cord-004738-vnz15x84	50	6	-	-	HYPH
cord-004738-vnz15x84	50	7	frame	frame	NN
cord-004738-vnz15x84	50	8	with	with	IN
cord-004738-vnz15x84	50	9	the	the	DT
cord-004738-vnz15x84	50	10	protease	protease	NN
cord-004738-vnz15x84	50	11	sequence	sequence	NN
cord-004738-vnz15x84	50	12	C	c	NN
cord-004738-vnz15x84	50	13	-	-	HYPH
cord-004738-vnz15x84	50	14	terminus	terminus	NN
cord-004738-vnz15x84	50	15	,	,	.
cord-004738-vnz15x84	51	1	a	a	DT
cord-004738-vnz15x84	51	2	green	green	JJ
cord-004738-vnz15x84	51	3	fluorescent	fluorescent	NN
cord-004738-vnz15x84	51	4	protein	protein	NN
cord-004738-vnz15x84	51	5	(	(	-LRB-
cord-004738-vnz15x84	51	6	GFP	GFP	NNP
cord-004738-vnz15x84	51	7	)	)	-RRB-
cord-004738-vnz15x84	51	8	ORF	ORF	NNP
cord-004738-vnz15x84	51	9	was	be	VBD
cord-004738-vnz15x84	51	10	also	also	RB
cord-004738-vnz15x84	51	11	inserted	insert	VBN
cord-004738-vnz15x84	51	12	,	,	,
cord-004738-vnz15x84	51	13	so	so	IN
cord-004738-vnz15x84	51	14	that	that	IN
cord-004738-vnz15x84	51	15	the	the	DT
cord-004738-vnz15x84	51	16	expressed	express	VBN
cord-004738-vnz15x84	51	17	protease	protease	NN
cord-004738-vnz15x84	51	18	would	would	MD
cord-004738-vnz15x84	51	19	have	have	VB
cord-004738-vnz15x84	51	20	a	a	DT
cord-004738-vnz15x84	51	21	higher	high	JJR
cord-004738-vnz15x84	51	22	molecular	molecular	JJ
cord-004738-vnz15x84	51	23	weight	weight	NN
cord-004738-vnz15x84	51	24	to	to	TO
cord-004738-vnz15x84	51	25	distinguish	distinguish	VB
cord-004738-vnz15x84	51	26	it	-PRON-	PRP
cord-004738-vnz15x84	51	27	from	from	IN
cord-004738-vnz15x84	51	28	its	-PRON-	PRP$
cord-004738-vnz15x84	51	29	substrate	substrate	NN
cord-004738-vnz15x84	51	30	in	in	IN
cord-004738-vnz15x84	51	31	immunoblot	immunoblot	NNP
cord-004738-vnz15x84	51	32	analysis	analysis	NN
cord-004738-vnz15x84	51	33	.	.	.
cord-004738-vnz15x84	52	1	This	this	DT
cord-004738-vnz15x84	52	2	expression	expression	NN
cord-004738-vnz15x84	52	3	plasmid	plasmid	NN
cord-004738-vnz15x84	52	4	was	be	VBD
cord-004738-vnz15x84	52	5	designated	designate	VBN
cord-004738-vnz15x84	52	6	as	as	IN
cord-004738-vnz15x84	52	7	pRVP	prvp	JJ
cord-004738-vnz15x84	52	8	(	(	-LRB-
cord-004738-vnz15x84	52	9	Fig	Fig	NNP
cord-004738-vnz15x84	52	10	.	.	.
cord-004738-vnz15x84	52	11	1A	1a	NN
cord-004738-vnz15x84	52	12	)	)	-RRB-
cord-004738-vnz15x84	52	13	.	.	.
cord-004738-vnz15x84	53	1	To	to	TO
cord-004738-vnz15x84	53	2	evaluate	evaluate	VB
cord-004738-vnz15x84	53	3	the	the	DT
cord-004738-vnz15x84	53	4	trans	trans	JJ
cord-004738-vnz15x84	53	5	-	-	JJ
cord-004738-vnz15x84	53	6	cleavage	cleavage	JJ
cord-004738-vnz15x84	53	7	activity	activity	NN
cord-004738-vnz15x84	53	8	of	of	IN
cord-004738-vnz15x84	53	9	RV	RV	NNP
cord-004738-vnz15x84	53	10	protease	protease	NN
cord-004738-vnz15x84	53	11	on	on	IN
cord-004738-vnz15x84	53	12	P200-derived	p200-derived	JJ
cord-004738-vnz15x84	53	13	substrates	substrate	NNS
cord-004738-vnz15x84	53	14	,	,	,
cord-004738-vnz15x84	53	15	a	a	DT
cord-004738-vnz15x84	53	16	series	series	NN
cord-004738-vnz15x84	53	17	of	of	IN
cord-004738-vnz15x84	53	18	RV	RV	NNP
cord-004738-vnz15x84	53	19	P200-related	p200-relate	VBD
cord-004738-vnz15x84	53	20	polypeptide	polypeptide	JJ
cord-004738-vnz15x84	53	21	expression	expression	NN
cord-004738-vnz15x84	53	22	plasmids	plasmid	NNS
cord-004738-vnz15x84	53	23	that	that	WDT
cord-004738-vnz15x84	53	24	express	express	VBP
cord-004738-vnz15x84	53	25	protein	protein	NN
cord-004738-vnz15x84	53	26	with	with	IN
cord-004738-vnz15x84	53	27	different	different	JJ
cord-004738-vnz15x84	53	28	N	n	CD
cord-004738-vnz15x84	53	29	-	-	HYPH
cord-004738-vnz15x84	53	30	terminal	terminal	NN
cord-004738-vnz15x84	53	31	lengths	length	NNS
cord-004738-vnz15x84	53	32	from	from	IN
cord-004738-vnz15x84	53	33	the	the	DT
cord-004738-vnz15x84	53	34	cleavage	cleavage	NN
cord-004738-vnz15x84	53	35	site	site	NN
cord-004738-vnz15x84	53	36	(	(	-LRB-
cord-004738-vnz15x84	53	37	72	72	CD
cord-004738-vnz15x84	53	38	,	,	,
cord-004738-vnz15x84	53	39	199	199	CD
cord-004738-vnz15x84	53	40	,	,	,
cord-004738-vnz15x84	53	41	309	309	CD
cord-004738-vnz15x84	53	42	,	,	,
cord-004738-vnz15x84	53	43	and	and	CC
cord-004738-vnz15x84	53	44	475	475	CD
cord-004738-vnz15x84	53	45	residues	residue	NNS
cord-004738-vnz15x84	53	46	)	)	-RRB-
cord-004738-vnz15x84	53	47	were	be	VBD
cord-004738-vnz15x84	53	48	constructed	construct	VBN
cord-004738-vnz15x84	53	49	.	.	.
cord-004738-vnz15x84	54	1	This	this	DT
cord-004738-vnz15x84	54	2	was	be	VBD
cord-004738-vnz15x84	54	3	achieved	achieve	VBN
cord-004738-vnz15x84	54	4	by	by	IN
cord-004738-vnz15x84	54	5	using	use	VBG
cord-004738-vnz15x84	54	6	a	a	DT
cord-004738-vnz15x84	54	7	previously	previously	RB
cord-004738-vnz15x84	54	8	well	well	RB
cord-004738-vnz15x84	54	9	-	-	HYPH
cord-004738-vnz15x84	54	10	established	establish	VBN
cord-004738-vnz15x84	54	11	RV	RV	NNP
cord-004738-vnz15x84	54	12	cDNA	cdna	NN
cord-004738-vnz15x84	54	13	template	template	NN
cord-004738-vnz15x84	54	14	,	,	,
cord-004738-vnz15x84	55	1	pBRM33-C1152S.	pbrm33-c1152s.	XX
cord-004738-vnz15x84	55	2	The	the	DT
cord-004738-vnz15x84	55	3	template	template	NN
cord-004738-vnz15x84	55	4	contains	contain	VBZ
cord-004738-vnz15x84	55	5	RV	RV	NNP
cord-004738-vnz15x84	55	6	cDNA	cdna	NN
cord-004738-vnz15x84	55	7	with	with	IN
cord-004738-vnz15x84	55	8	the	the	DT
cord-004738-vnz15x84	55	9	cleavage	cleavage	NN
cord-004738-vnz15x84	55	10	site	site	NN
cord-004738-vnz15x84	55	11	(	(	-LRB-
cord-004738-vnz15x84	55	12	N	N	NNP
cord-004738-vnz15x84	55	13	-SRGG/	-SRGG/	HYPH
cord-004738-vnz15x84	55	14	G	G	NNP
cord-004738-vnz15x84	55	15	-	-	HYPH
cord-004738-vnz15x84	55	16	C	C	NNP
cord-004738-vnz15x84	55	17	)	)	-RRB-
cord-004738-vnz15x84	55	18	unmodified	unmodified	JJ
cord-004738-vnz15x84	55	19	but	but	CC
cord-004738-vnz15x84	55	20	lacks	lack	VBZ
cord-004738-vnz15x84	55	21	the	the	DT
cord-004738-vnz15x84	55	22	protease	protease	NN
cord-004738-vnz15x84	55	23	activity	activity	NN
cord-004738-vnz15x84	55	24	due	due	IN
cord-004738-vnz15x84	55	25	to	to	IN
cord-004738-vnz15x84	55	26	a	a	DT
cord-004738-vnz15x84	55	27	substitution	substitution	NN
cord-004738-vnz15x84	55	28	at	at	IN
cord-004738-vnz15x84	55	29	the	the	DT
cord-004738-vnz15x84	55	30	catalytic	catalytic	JJ
cord-004738-vnz15x84	55	31	activity	activity	NN
cord-004738-vnz15x84	55	32	residue	residue	NN
cord-004738-vnz15x84	55	33	(	(	-LRB-
cord-004738-vnz15x84	55	34	C1152S	C1152S	NNP
cord-004738-vnz15x84	55	35	)	)	-RRB-
cord-004738-vnz15x84	55	36	of	of	IN
cord-004738-vnz15x84	55	37	the	the	DT
cord-004738-vnz15x84	55	38	enzyme	enzyme	NN
cord-004738-vnz15x84	55	39	[	[	-LRB-
cord-004738-vnz15x84	55	40	8	8	CD
cord-004738-vnz15x84	55	41	]	]	-RRB-
cord-004738-vnz15x84	55	42	.	.	.
cord-004738-vnz15x84	56	1	Desired	desire	VBN
cord-004738-vnz15x84	56	2	DNA	dna	NN
cord-004738-vnz15x84	56	3	segments	segment	NNS
cord-004738-vnz15x84	56	4	of	of	IN
cord-004738-vnz15x84	56	5	pBRM33-C1152S	pbrm33-c1152s	NN
cord-004738-vnz15x84	56	6	template	template	NN
cord-004738-vnz15x84	56	7	with	with	IN
cord-004738-vnz15x84	56	8	the	the	DT
cord-004738-vnz15x84	56	9	same	same	JJ
cord-004738-vnz15x84	56	10	C	c	NN
cord-004738-vnz15x84	56	11	-	-	HYPH
cord-004738-vnz15x84	56	12	terminus	terminus	NN
cord-004738-vnz15x84	56	13	(	(	-LRB-
cord-004738-vnz15x84	56	14	amino	amino	NN
cord-004738-vnz15x84	56	15	acid	acid	NN
cord-004738-vnz15x84	56	16	position	position	NN
cord-004738-vnz15x84	56	17	1548	1548	CD
cord-004738-vnz15x84	56	18	)	)	-RRB-
cord-004738-vnz15x84	56	19	but	but	CC
cord-004738-vnz15x84	56	20	varying	vary	VBG
cord-004738-vnz15x84	56	21	in	in	IN
cord-004738-vnz15x84	56	22	length	length	NN
cord-004738-vnz15x84	56	23	at	at	IN
cord-004738-vnz15x84	56	24	the	the	DT
cord-004738-vnz15x84	56	25	N	n	CD
cord-004738-vnz15x84	56	26	-	-	HYPH
cord-004738-vnz15x84	56	27	terminal	terminal	JJ
cord-004738-vnz15x84	56	28	end	end	NN
cord-004738-vnz15x84	56	29	were	be	VBD
cord-004738-vnz15x84	56	30	amplified	amplify	VBN
cord-004738-vnz15x84	56	31	using	use	VBG
cord-004738-vnz15x84	56	32	appropriate	appropriate	JJ
cord-004738-vnz15x84	56	33	sets	set	NNS
cord-004738-vnz15x84	56	34	of	of	IN
cord-004738-vnz15x84	56	35	primers	primer	NNS
cord-004738-vnz15x84	56	36	(	(	-LRB-
cord-004738-vnz15x84	56	37	Table	table	NN
cord-004738-vnz15x84	56	38	1	1	CD
cord-004738-vnz15x84	56	39	)	)	-RRB-
cord-004738-vnz15x84	56	40	and	and	CC
cord-004738-vnz15x84	56	41	cloned	clone	VBN
cord-004738-vnz15x84	56	42	at	at	IN
cord-004738-vnz15x84	56	43	the	the	DT
cord-004738-vnz15x84	56	44	BamHI	bamhi	NN
cord-004738-vnz15x84	56	45	and	and	CC
cord-004738-vnz15x84	56	46	EcoRI	ecori	NN
cord-004738-vnz15x84	56	47	sites	site	NNS
cord-004738-vnz15x84	56	48	of	of	IN
cord-004738-vnz15x84	56	49	the	the	DT
cord-004738-vnz15x84	56	50	plasmid	plasmid	NN
cord-004738-vnz15x84	56	51	vector	vector	NN
cord-004738-vnz15x84	56	52	described	describe	VBN
cord-004738-vnz15x84	56	53	above	above	RB
cord-004738-vnz15x84	56	54	.	.	.
cord-004738-vnz15x84	57	1	The	the	DT
cord-004738-vnz15x84	57	2	plasmids	plasmid	NNS
cord-004738-vnz15x84	57	3	were	be	VBD
cord-004738-vnz15x84	57	4	designated	designate	VBN
cord-004738-vnz15x84	57	5	,	,	,
cord-004738-vnz15x84	57	6	pRVS-827	pRVS-827	NNS
cord-004738-vnz15x84	57	7	-	-	HYPH
cord-004738-vnz15x84	57	8	1548	1548	CD
cord-004738-vnz15x84	57	9	,	,	,
cord-004738-vnz15x84	57	10	pRVS-994	prvs-994	CD
cord-004738-vnz15x84	57	11	-	-	SYM
cord-004738-vnz15x84	57	12	1548	1548	CD
cord-004738-vnz15x84	57	13	,	,	,
cord-004738-vnz15x84	57	14	pRVS-1102	pRVS-1102	NNS
cord-004738-vnz15x84	57	15	-	-	HYPH
cord-004738-vnz15x84	57	16	1548	1548	CD
cord-004738-vnz15x84	57	17	,	,	,
cord-004738-vnz15x84	57	18	and	and	CC
cord-004738-vnz15x84	57	19	pRVS-1228	prvs-1228	NN
cord-004738-vnz15x84	57	20	-	-	HYPH
cord-004738-vnz15x84	57	21	1548	1548	CD
cord-004738-vnz15x84	57	22	,	,	,
cord-004738-vnz15x84	57	23	producing	produce	VBG
cord-004738-vnz15x84	57	24	proteins	protein	NNS
cord-004738-vnz15x84	57	25	with	with	IN
cord-004738-vnz15x84	57	26	475-	475-	CD
cord-004738-vnz15x84	57	27	,	,	,
cord-004738-vnz15x84	57	28	309-	309-	CD
cord-004738-vnz15x84	57	29	,	,	,
cord-004738-vnz15x84	57	30	199-	199-	CD
cord-004738-vnz15x84	57	31	,	,	,
cord-004738-vnz15x84	57	32	and	and	CC
cord-004738-vnz15x84	57	33	72-residue	72-residue	CD
cord-004738-vnz15x84	57	34	N	n	CD
cord-004738-vnz15x84	57	35	-	-	HYPH
cord-004738-vnz15x84	57	36	terminal	terminal	NN
cord-004738-vnz15x84	57	37	lengths	length	NNS
cord-004738-vnz15x84	57	38	from	from	IN
cord-004738-vnz15x84	57	39	the	the	DT
cord-004738-vnz15x84	57	40	cleavage	cleavage	NN
cord-004738-vnz15x84	57	41	site	site	NN
cord-004738-vnz15x84	57	42	,	,	,
cord-004738-vnz15x84	57	43	respectively	respectively	RB
cord-004738-vnz15x84	57	44	(	(	-LRB-
cord-004738-vnz15x84	57	45	Fig	fig	NN
cord-004738-vnz15x84	57	46	.	.	.
cord-004738-vnz15x84	57	47	1B	1b	CD
cord-004738-vnz15x84	57	48	)	)	-RRB-
cord-004738-vnz15x84	57	49	.	.	.
cord-004738-vnz15x84	58	1	To	to	TO
cord-004738-vnz15x84	58	2	test	test	VB
cord-004738-vnz15x84	58	3	whether	whether	IN
cord-004738-vnz15x84	58	4	replacement	replacement	NN
cord-004738-vnz15x84	58	5	of	of	IN
cord-004738-vnz15x84	58	6	P200-related	p200-related	JJ
cord-004738-vnz15x84	58	7	sequences	sequence	NNS
cord-004738-vnz15x84	58	8	C	c	NN
cord-004738-vnz15x84	58	9	-	-	HYPH
cord-004738-vnz15x84	58	10	terminal	terminal	NN
cord-004738-vnz15x84	58	11	to	to	IN
cord-004738-vnz15x84	58	12	the	the	DT
cord-004738-vnz15x84	58	13	cleavage	cleavage	NN
cord-004738-vnz15x84	58	14	site	site	NN
cord-004738-vnz15x84	58	15	by	by	IN
cord-004738-vnz15x84	58	16	non	non	JJ
cord-004738-vnz15x84	58	17	-	-	JJ
cord-004738-vnz15x84	58	18	rubella	rubella	JJ
cord-004738-vnz15x84	58	19	sequences	sequence	NNS
cord-004738-vnz15x84	58	20	affect	affect	VBP
cord-004738-vnz15x84	58	21	RV	RV	NNP
cord-004738-vnz15x84	58	22	protease	protease	NN
cord-004738-vnz15x84	58	23	trans	trans	NN
cord-004738-vnz15x84	58	24	-	-	NN
cord-004738-vnz15x84	58	25	activity	activity	NN
cord-004738-vnz15x84	58	26	,	,	,
cord-004738-vnz15x84	58	27	the	the	DT
cord-004738-vnz15x84	58	28	plasmid	plasmid	NN
cord-004738-vnz15x84	58	29	pRVS	prvs	JJ
cord-004738-vnz15x84	58	30	-	-	HYPH
cord-004738-vnz15x84	58	31	GFP	GFP	NNP
cord-004738-vnz15x84	58	32	,	,	,
cord-004738-vnz15x84	58	33	which	which	WDT
cord-004738-vnz15x84	58	34	contains	contain	VBZ
cord-004738-vnz15x84	58	35	PCR	PCR	NNP
cord-004738-vnz15x84	58	36	-	-	HYPH
cord-004738-vnz15x84	58	37	amplified	amplify	VBN
cord-004738-vnz15x84	58	38	RV	RV	NNP
cord-004738-vnz15x84	58	39	protease	protease	NN
cord-004738-vnz15x84	58	40	substrate	substrate	NN
cord-004738-vnz15x84	58	41	sequences	sequence	NNS
cord-004738-vnz15x84	58	42	representing	represent	VBG
cord-004738-vnz15x84	58	43	polypeptide	polypeptide	NN
cord-004738-vnz15x84	58	44	residues	residue	NNS
cord-004738-vnz15x84	59	1	827	827	CD
cord-004738-vnz15x84	59	2	-	-	SYM
cord-004738-vnz15x84	59	3	1306	1306	CD
cord-004738-vnz15x84	60	1	(	(	-LRB-
cord-004738-vnz15x84	60	2	residues	residue	NNS
cord-004738-vnz15x84	60	3	1302	1302	CD
cord-004738-vnz15x84	60	4	-	-	SYM
cord-004738-vnz15x84	60	5	1306	1306	CD
cord-004738-vnz15x84	60	6	represent	represent	VBP
cord-004738-vnz15x84	60	7	the	the	DT
cord-004738-vnz15x84	60	8	C	C	NNP
cord-004738-vnz15x84	60	9	-	-	HYPH
cord-004738-vnz15x84	60	10	terminal	terminal	NN
cord-004738-vnz15x84	60	11	side	side	NN
cord-004738-vnz15x84	60	12	of	of	IN
cord-004738-vnz15x84	60	13	the	the	DT
cord-004738-vnz15x84	60	14	cleavage	cleavage	NN
cord-004738-vnz15x84	60	15	site	site	NN
cord-004738-vnz15x84	60	16	)	)	-RRB-
cord-004738-vnz15x84	60	17	,	,	,
cord-004738-vnz15x84	60	18	fused	fuse	VBN
cord-004738-vnz15x84	60	19	at	at	IN
cord-004738-vnz15x84	60	20	its	-PRON-	PRP$
cord-004738-vnz15x84	60	21	C	c	NN
cord-004738-vnz15x84	60	22	-	-	HYPH
cord-004738-vnz15x84	60	23	terminus	terminus	NN
cord-004738-vnz15x84	60	24	to	to	IN
cord-004738-vnz15x84	60	25	GFP	GFP	NNP
cord-004738-vnz15x84	60	26	ORF	ORF	NNP
cord-004738-vnz15x84	60	27	in	in	IN
cord-004738-vnz15x84	60	28	-	-	HYPH
cord-004738-vnz15x84	60	29	frame	frame	NN
cord-004738-vnz15x84	60	30	,	,	,
cord-004738-vnz15x84	60	31	was	be	VBD
cord-004738-vnz15x84	60	32	created	create	VBN
cord-004738-vnz15x84	60	33	(	(	-LRB-
cord-004738-vnz15x84	60	34	Fig	Fig	NNP
cord-004738-vnz15x84	60	35	.	.	.
cord-004738-vnz15x84	60	36	1B	1b	CD
cord-004738-vnz15x84	60	37	)	)	-RRB-
cord-004738-vnz15x84	60	38	.	.	.
cord-004738-vnz15x84	61	1	Similarly	similarly	RB
cord-004738-vnz15x84	61	2	,	,	,
cord-004738-vnz15x84	61	3	to	to	TO
cord-004738-vnz15x84	61	4	test	test	VB
cord-004738-vnz15x84	61	5	whether	whether	IN
cord-004738-vnz15x84	61	6	a	a	DT
cord-004738-vnz15x84	61	7	heterologous	heterologous	JJ
cord-004738-vnz15x84	61	8	sequence	sequence	NN
cord-004738-vnz15x84	61	9	on	on	IN
cord-004738-vnz15x84	61	10	the	the	DT
cord-004738-vnz15x84	61	11	N	n	CD
cord-004738-vnz15x84	61	12	-	-	HYPH
cord-004738-vnz15x84	61	13	terminal	terminal	JJ
cord-004738-vnz15x84	61	14	side	side	NN
cord-004738-vnz15x84	61	15	of	of	IN
cord-004738-vnz15x84	61	16	the	the	DT
cord-004738-vnz15x84	61	17	cleavage	cleavage	NN
cord-004738-vnz15x84	61	18	site	site	NN
cord-004738-vnz15x84	61	19	affects	affect	VBZ
cord-004738-vnz15x84	61	20	substrate	substrate	NN
cord-004738-vnz15x84	61	21	recognition	recognition	NN
cord-004738-vnz15x84	61	22	by	by	IN
cord-004738-vnz15x84	61	23	the	the	DT
cord-004738-vnz15x84	61	24	protease	protease	NN
cord-004738-vnz15x84	61	25	trans	trans	NN
cord-004738-vnz15x84	61	26	-	-	NN
cord-004738-vnz15x84	61	27	activity	activity	NN
cord-004738-vnz15x84	61	28	,	,	,
cord-004738-vnz15x84	61	29	GFP	GFP	NNP
cord-004738-vnz15x84	61	30	ORF	ORF	NNP
cord-004738-vnz15x84	61	31	was	be	VBD
cord-004738-vnz15x84	61	32	fused	fuse	VBN
cord-004738-vnz15x84	61	33	in	in	IN
cord-004738-vnz15x84	61	34	-	-	HYPH
cord-004738-vnz15x84	61	35	frame	frame	NN
cord-004738-vnz15x84	61	36	at	at	IN
cord-004738-vnz15x84	61	37	the	the	DT
cord-004738-vnz15x84	61	38	N	n	CD
cord-004738-vnz15x84	61	39	-	-	HYPH
cord-004738-vnz15x84	61	40	terminus	terminus	NN
cord-004738-vnz15x84	61	41	of	of	IN
cord-004738-vnz15x84	61	42	the	the	DT
cord-004738-vnz15x84	61	43	rubella	rubella	NN
cord-004738-vnz15x84	61	44	sequence	sequence	NN
cord-004738-vnz15x84	61	45	in	in	IN
cord-004738-vnz15x84	61	46	the	the	DT
cord-004738-vnz15x84	61	47	plasmid	plasmid	NN
cord-004738-vnz15x84	61	48	pRVS-1102	pRVS-1102	NNP
cord-004738-vnz15x84	61	49	-	-	:
cord-004738-vnz15x84	61	50	1548	1548	CD
cord-004738-vnz15x84	61	51	to	to	TO
cord-004738-vnz15x84	61	52	create	create	VB
cord-004738-vnz15x84	61	53	pRVS	prvs	JJ
cord-004738-vnz15x84	61	54	-	-	HYPH
cord-004738-vnz15x84	61	55	GFP-1102	gfp-1102	NN
cord-004738-vnz15x84	61	56	-	-	HYPH
cord-004738-vnz15x84	61	57	1548	1548	CD
cord-004738-vnz15x84	62	1	(	(	-LRB-
cord-004738-vnz15x84	62	2	Fig	Fig	NNP
cord-004738-vnz15x84	62	3	.	.	.
cord-004738-vnz15x84	62	4	1B	1b	CD
cord-004738-vnz15x84	62	5	)	)	-RRB-
cord-004738-vnz15x84	62	6	.	.	.
cord-004738-vnz15x84	63	1	The	the	DT
cord-004738-vnz15x84	63	2	template	template	NN
cord-004738-vnz15x84	63	3	for	for	IN
cord-004738-vnz15x84	63	4	the	the	DT
cord-004738-vnz15x84	63	5	PCR	PCR	NNP
cord-004738-vnz15x84	63	6	in	in	IN
cord-004738-vnz15x84	63	7	both	both	DT
cord-004738-vnz15x84	63	8	cases	case	NNS
cord-004738-vnz15x84	63	9	was	be	VBD
cord-004738-vnz15x84	63	10	pBRM33-C1152S.	pBRM33-C1152S.	NNS
cord-004738-vnz15x84	63	11	(	(	-LRB-
cord-004738-vnz15x84	63	12	A	a	NN
cord-004738-vnz15x84	63	13	)	)	-RRB-
cord-004738-vnz15x84	64	1	Shows	show	VBZ
cord-004738-vnz15x84	64	2	pBRM33-G1301S	pbrm33-g1301s	NN
cord-004738-vnz15x84	64	3	-	-	HYPH
cord-004738-vnz15x84	64	4	derived	derive	VBN
cord-004738-vnz15x84	64	5	P200	p200	NN
cord-004738-vnz15x84	64	6	that	that	WDT
cord-004738-vnz15x84	64	7	was	be	VBD
cord-004738-vnz15x84	64	8	used	use	VBN
cord-004738-vnz15x84	64	9	as	as	IN
cord-004738-vnz15x84	64	10	a	a	DT
cord-004738-vnz15x84	64	11	PCR	pcr	NN
cord-004738-vnz15x84	64	12	-	-	HYPH
cord-004738-vnz15x84	64	13	template	template	NN
cord-004738-vnz15x84	64	14	to	to	TO
cord-004738-vnz15x84	64	15	clone	clone	VB
cord-004738-vnz15x84	64	16	the	the	DT
cord-004738-vnz15x84	64	17	trans	trans	JJ
cord-004738-vnz15x84	64	18	-	-	JJ
cord-004738-vnz15x84	64	19	active	active	JJ
cord-004738-vnz15x84	64	20	protease	protease	NN
cord-004738-vnz15x84	64	21	region	region	NN
cord-004738-vnz15x84	64	22	.	.	.
cord-004738-vnz15x84	65	1	The	the	DT
cord-004738-vnz15x84	65	2	P150	P150	NNPS
cord-004738-vnz15x84	65	3	and	and	CC
cord-004738-vnz15x84	65	4	P90	P90	NNP
cord-004738-vnz15x84	65	5	domains	domain	NNS
cord-004738-vnz15x84	65	6	of	of	IN
cord-004738-vnz15x84	65	7	P200	P200	NNS
cord-004738-vnz15x84	65	8	are	be	VBP
cord-004738-vnz15x84	65	9	indicated	indicate	VBN
cord-004738-vnz15x84	65	10	.	.	.
cord-004738-vnz15x84	66	1	The	the	DT
cord-004738-vnz15x84	66	2	protease	protease	NN
cord-004738-vnz15x84	66	3	catalytic	catalytic	JJ
cord-004738-vnz15x84	66	4	residue	residue	NN
cord-004738-vnz15x84	66	5	,	,	,
cord-004738-vnz15x84	66	6	Cys	Cys	NNP
cord-004738-vnz15x84	66	7	at	at	IN
cord-004738-vnz15x84	66	8	amino	amino	NN
cord-004738-vnz15x84	66	9	acid	acid	NN
cord-004738-vnz15x84	66	10	1152	1152	CD
cord-004738-vnz15x84	66	11	(	(	-LRB-
cord-004738-vnz15x84	66	12	C1152	C1152	NNP
cord-004738-vnz15x84	66	13	)	)	-RRB-
cord-004738-vnz15x84	66	14	and	and	CC
cord-004738-vnz15x84	66	15	a	a	DT
cord-004738-vnz15x84	66	16	cleavage	cleavage	NN
cord-004738-vnz15x84	66	17	site	site	NN
cord-004738-vnz15x84	66	18	substitution	substitution	NN
cord-004738-vnz15x84	66	19	mutation	mutation	NN
cord-004738-vnz15x84	66	20	(	(	-LRB-
cord-004738-vnz15x84	66	21	Gly	Gly	NNP
cord-004738-vnz15x84	66	22	to	to	IN
cord-004738-vnz15x84	66	23	Ser	Ser	NNP
cord-004738-vnz15x84	66	24	)	)	-RRB-
cord-004738-vnz15x84	66	25	at	at	IN
cord-004738-vnz15x84	66	26	P150	P150	NNP
cord-004738-vnz15x84	66	27	C	C	NNP
cord-004738-vnz15x84	66	28	-	-	HYPH
cord-004738-vnz15x84	66	29	terminal	terminal	NN
cord-004738-vnz15x84	66	30	residue	residue	NN
cord-004738-vnz15x84	66	31	,	,	,
cord-004738-vnz15x84	66	32	1301	1301	CD
cord-004738-vnz15x84	66	33	(	(	-LRB-
cord-004738-vnz15x84	66	34	G1301S	G1301S	NNP
cord-004738-vnz15x84	66	35	,	,	,
cord-004738-vnz15x84	66	36	represented	represent	VBN
cord-004738-vnz15x84	66	37	by	by	IN
cord-004738-vnz15x84	66	38	'	'	``
cord-004738-vnz15x84	66	39	X	x	NN
cord-004738-vnz15x84	66	40	'	'	''
cord-004738-vnz15x84	66	41	in	in	IN
cord-004738-vnz15x84	66	42	the	the	DT
cord-004738-vnz15x84	66	43	P150	p150	CD
cord-004738-vnz15x84	66	44	box	box	NN
cord-004738-vnz15x84	66	45	and	and	CC
cord-004738-vnz15x84	66	46	by	by	IN
cord-004738-vnz15x84	66	47	a	a	DT
cord-004738-vnz15x84	66	48	black	black	JJ
cord-004738-vnz15x84	66	49	circle	circle	NN
cord-004738-vnz15x84	66	50	on	on	IN
cord-004738-vnz15x84	66	51	the	the	DT
cord-004738-vnz15x84	66	52	top	top	NN
cord-004738-vnz15x84	66	53	)	)	-RRB-
cord-004738-vnz15x84	66	54	are	be	VBP
cord-004738-vnz15x84	66	55	as	as	IN
cord-004738-vnz15x84	66	56	shown	show	VBN
cord-004738-vnz15x84	66	57	.	.	.
cord-004738-vnz15x84	67	1	The	the	DT
cord-004738-vnz15x84	67	2	mutation	mutation	NN
cord-004738-vnz15x84	67	3	eliminates	eliminate	VBZ
cord-004738-vnz15x84	67	4	the	the	DT
cord-004738-vnz15x84	67	5	cis	cis	NN
cord-004738-vnz15x84	67	6	-	-	HYPH
cord-004738-vnz15x84	67	7	activity	activity	NN
cord-004738-vnz15x84	67	8	of	of	IN
cord-004738-vnz15x84	67	9	the	the	DT
cord-004738-vnz15x84	67	10	protease	protease	NN
cord-004738-vnz15x84	67	11	,	,	,
cord-004738-vnz15x84	67	12	leaving	leave	VBG
cord-004738-vnz15x84	67	13	its	-PRON-	PRP$
cord-004738-vnz15x84	67	14	trans	trans	JJ
cord-004738-vnz15x84	67	15	-	-	NN
cord-004738-vnz15x84	67	16	activity	activity	NN
cord-004738-vnz15x84	67	17	intact	intact	JJ
cord-004738-vnz15x84	67	18	[	[	-LRB-
cord-004738-vnz15x84	67	19	8	8	CD
cord-004738-vnz15x84	67	20	]	]	-RRB-
cord-004738-vnz15x84	67	21	.	.	.
cord-004738-vnz15x84	68	1	Numbers	number	NNS
cord-004738-vnz15x84	68	2	(	(	-LRB-
cord-004738-vnz15x84	68	3	1	1	CD
cord-004738-vnz15x84	68	4	-	-	CD
cord-004738-vnz15x84	68	5	2115	2115	CD
cord-004738-vnz15x84	68	6	)	)	-RRB-
cord-004738-vnz15x84	68	7	on	on	IN
cord-004738-vnz15x84	68	8	the	the	DT
cord-004738-vnz15x84	68	9	side	side	NN
cord-004738-vnz15x84	68	10	represent	represent	VBP
cord-004738-vnz15x84	68	11	amino	amino	NN
cord-004738-vnz15x84	68	12	acid	acid	NN
cord-004738-vnz15x84	68	13	residues	residue	NNS
cord-004738-vnz15x84	68	14	of	of	IN
cord-004738-vnz15x84	68	15	P200	p200	NN
cord-004738-vnz15x84	68	16	.	.	.
cord-004738-vnz15x84	69	1	The	the	DT
cord-004738-vnz15x84	69	2	trans	trans	JJ
cord-004738-vnz15x84	69	3	-	-	JJ
cord-004738-vnz15x84	69	4	active	active	JJ
cord-004738-vnz15x84	69	5	protease	protease	NN
cord-004738-vnz15x84	69	6	domain	domain	NN
cord-004738-vnz15x84	69	7	spans	span	VBZ
cord-004738-vnz15x84	69	8	from	from	IN
cord-004738-vnz15x84	69	9	amino	amino	NN
cord-004738-vnz15x84	69	10	acid	acid	NN
cord-004738-vnz15x84	69	11	position	position	NN
cord-004738-vnz15x84	69	12	827	827	CD
cord-004738-vnz15x84	69	13	to	to	IN
cord-004738-vnz15x84	69	14	1301	1301	CD
cord-004738-vnz15x84	69	15	[	[	-LRB-
cord-004738-vnz15x84	69	16	8	8	CD
cord-004738-vnz15x84	69	17	]	]	-RRB-
cord-004738-vnz15x84	69	18	.	.	.
cord-004738-vnz15x84	70	1	Expression	expression	NN
cord-004738-vnz15x84	70	2	plasmid	plasmid	NN
cord-004738-vnz15x84	70	3	,	,	,
cord-004738-vnz15x84	70	4	pRVP	pRVP	NNP
cord-004738-vnz15x84	70	5	contains	contain	VBZ
cord-004738-vnz15x84	70	6	this	this	DT
cord-004738-vnz15x84	70	7	protease	protease	NN
cord-004738-vnz15x84	70	8	domain	domain	NN
cord-004738-vnz15x84	70	9	in	in	IN
cord-004738-vnz15x84	70	10	-	-	HYPH
cord-004738-vnz15x84	70	11	frame	frame	NN
cord-004738-vnz15x84	70	12	flanked	flank	VBN
cord-004738-vnz15x84	70	13	by	by	IN
cord-004738-vnz15x84	70	14	an	an	DT
cord-004738-vnz15x84	70	15	N	n	CD
cord-004738-vnz15x84	70	16	-	-	HYPH
cord-004738-vnz15x84	70	17	terminal	terminal	NN
cord-004738-vnz15x84	70	18	Xpress	Xpress	NNP
cord-004738-vnz15x84	70	19	epitope	epitope	NN
cord-004738-vnz15x84	70	20	tag	tag	NN
cord-004738-vnz15x84	70	21	(	(	-LRB-
cord-004738-vnz15x84	70	22	hatched	hatch	VBN
cord-004738-vnz15x84	70	23	box	box	NN
cord-004738-vnz15x84	70	24	)	)	-RRB-
cord-004738-vnz15x84	70	25	and	and	CC
cord-004738-vnz15x84	70	26	a	a	DT
cord-004738-vnz15x84	70	27	C	c	NN
cord-004738-vnz15x84	70	28	-	-	HYPH
cord-004738-vnz15x84	70	29	terminal	terminal	NN
cord-004738-vnz15x84	70	30	GFP	GFP	NNP
cord-004738-vnz15x84	70	31	ORF	ORF	NNP
cord-004738-vnz15x84	70	32	.	.	.
cord-004738-vnz15x84	71	1	(	(	-LRB-
cord-004738-vnz15x84	71	2	B	b	NN
cord-004738-vnz15x84	71	3	)	)	-RRB-
cord-004738-vnz15x84	71	4	Shows	show	VBZ
cord-004738-vnz15x84	71	5	pBRM33-C1152S	pbrm33-c1152s	NN
cord-004738-vnz15x84	71	6	-	-	HYPH
cord-004738-vnz15x84	71	7	derived	derive	VBN
cord-004738-vnz15x84	71	8	P200	p200	NN
cord-004738-vnz15x84	71	9	used	use	VBN
cord-004738-vnz15x84	71	10	as	as	IN
cord-004738-vnz15x84	71	11	a	a	DT
cord-004738-vnz15x84	71	12	PCR	pcr	NN
cord-004738-vnz15x84	71	13	-	-	HYPH
cord-004738-vnz15x84	71	14	template	template	NN
cord-004738-vnz15x84	71	15	to	to	TO
cord-004738-vnz15x84	71	16	produce	produce	VB
cord-004738-vnz15x84	71	17	a	a	DT
cord-004738-vnz15x84	71	18	series	series	NN
cord-004738-vnz15x84	71	19	of	of	IN
cord-004738-vnz15x84	71	20	deletion	deletion	NN
cord-004738-vnz15x84	71	21	substrates	substrate	NNS
cord-004738-vnz15x84	71	22	.	.	.
cord-004738-vnz15x84	72	1	The	the	DT
cord-004738-vnz15x84	72	2	P150	P150	NNPS
cord-004738-vnz15x84	72	3	and	and	CC
cord-004738-vnz15x84	72	4	P90	P90	NNP
cord-004738-vnz15x84	72	5	domains	domain	NNS
cord-004738-vnz15x84	72	6	on	on	IN
cord-004738-vnz15x84	72	7	P200	p200	NN
cord-004738-vnz15x84	72	8	were	be	VBD
cord-004738-vnz15x84	72	9	as	as	IN
cord-004738-vnz15x84	72	10	shown	show	VBN
cord-004738-vnz15x84	72	11	.	.	.
cord-004738-vnz15x84	73	1	To	to	TO
cord-004738-vnz15x84	73	2	serve	serve	VB
cord-004738-vnz15x84	73	3	as	as	IN
cord-004738-vnz15x84	73	4	homologous	homologous	JJ
cord-004738-vnz15x84	73	5	substrates	substrate	NNS
cord-004738-vnz15x84	73	6	,	,	,
cord-004738-vnz15x84	73	7	the	the	DT
cord-004738-vnz15x84	73	8	protease	protease	NN
cord-004738-vnz15x84	73	9	activity	activity	NN
cord-004738-vnz15x84	73	10	was	be	VBD
cord-004738-vnz15x84	73	11	eliminated	eliminate	VBN
cord-004738-vnz15x84	73	12	due	due	IN
cord-004738-vnz15x84	73	13	to	to	IN
cord-004738-vnz15x84	73	14	a	a	DT
cord-004738-vnz15x84	73	15	substitution	substitution	NN
cord-004738-vnz15x84	73	16	at	at	IN
cord-004738-vnz15x84	73	17	the	the	DT
cord-004738-vnz15x84	73	18	catalytic	catalytic	JJ
cord-004738-vnz15x84	73	19	residue	residue	NN
cord-004738-vnz15x84	73	20	,	,	,
cord-004738-vnz15x84	73	21	Cys-1152	cys-1152	NN
cord-004738-vnz15x84	73	22	to	to	IN
cord-004738-vnz15x84	73	23	a	a	DT
cord-004738-vnz15x84	73	24	Ser	Ser	NNP
cord-004738-vnz15x84	73	25	[	[	-LRB-
cord-004738-vnz15x84	73	26	8	8	CD
cord-004738-vnz15x84	73	27	]	]	-RRB-
cord-004738-vnz15x84	73	28	(	(	-LRB-
cord-004738-vnz15x84	73	29	C1152S	c1152s	XX
cord-004738-vnz15x84	73	30	,	,	,
cord-004738-vnz15x84	73	31	represented	represent	VBN
cord-004738-vnz15x84	73	32	by	by	IN
cord-004738-vnz15x84	73	33	'	'	``
cord-004738-vnz15x84	73	34	X	x	NN
cord-004738-vnz15x84	73	35	'	'	''
cord-004738-vnz15x84	73	36	and	and	CC
cord-004738-vnz15x84	73	37	by	by	IN
cord-004738-vnz15x84	73	38	an	an	DT
cord-004738-vnz15x84	73	39	open	open	JJ
cord-004738-vnz15x84	73	40	circle	circle	NN
cord-004738-vnz15x84	73	41	on	on	IN
cord-004738-vnz15x84	73	42	the	the	DT
cord-004738-vnz15x84	73	43	top	top	NN
cord-004738-vnz15x84	73	44	)	)	-RRB-
cord-004738-vnz15x84	73	45	but	but	CC
cord-004738-vnz15x84	73	46	with	with	IN
cord-004738-vnz15x84	73	47	an	an	DT
cord-004738-vnz15x84	73	48	intact	intact	JJ
cord-004738-vnz15x84	73	49	cleavage	cleavage	NN
cord-004738-vnz15x84	73	50	site	site	NN
cord-004738-vnz15x84	73	51	at	at	IN
cord-004738-vnz15x84	73	52	the	the	DT
cord-004738-vnz15x84	73	53	P150	p150	CD
cord-004738-vnz15x84	73	54	C	c	NN
cord-004738-vnz15x84	73	55	-	-	HYPH
cord-004738-vnz15x84	73	56	terminal	terminal	NN
cord-004738-vnz15x84	73	57	residue	residue	NN
cord-004738-vnz15x84	73	58	,	,	,
cord-004738-vnz15x84	73	59	1301	1301	CD
cord-004738-vnz15x84	73	60	(	(	-LRB-
cord-004738-vnz15x84	73	61	G1301	G1301	NNP
cord-004738-vnz15x84	73	62	)	)	-RRB-
cord-004738-vnz15x84	73	63	as	as	IN
cord-004738-vnz15x84	73	64	shown	show	VBN
cord-004738-vnz15x84	73	65	.	.	.
cord-004738-vnz15x84	74	1	Expression	expression	NN
cord-004738-vnz15x84	74	2	plasmids	plasmid	NNS
cord-004738-vnz15x84	74	3	containing	contain	VBG
cord-004738-vnz15x84	74	4	RV	RV	NNP
cord-004738-vnz15x84	74	5	P200-related	p200-related	JJ
cord-004738-vnz15x84	74	6	cDNA	cdna	NN
cord-004738-vnz15x84	74	7	pRVS-827	pRVS-827	NNP
cord-004738-vnz15x84	74	8	-	-	HYPH
cord-004738-vnz15x84	74	9	1548	1548	CD
cord-004738-vnz15x84	74	10	,	,	,
cord-004738-vnz15x84	74	11	pRVS-994	prvs-994	CD
cord-004738-vnz15x84	74	12	-	-	SYM
cord-004738-vnz15x84	74	13	1548	1548	CD
cord-004738-vnz15x84	74	14	,	,	,
cord-004738-vnz15x84	74	15	pRVS-1102	pRVS-1102	NNS
cord-004738-vnz15x84	74	16	-	-	HYPH
cord-004738-vnz15x84	74	17	1548	1548	CD
cord-004738-vnz15x84	74	18	,	,	,
cord-004738-vnz15x84	74	19	and	and	CC
cord-004738-vnz15x84	74	20	pRVS-1228	prvs-1228	NN
cord-004738-vnz15x84	74	21	-	-	HYPH
cord-004738-vnz15x84	74	22	1548	1548	CD
cord-004738-vnz15x84	75	1	(	(	-LRB-
cord-004738-vnz15x84	75	2	numbers	number	NNS
cord-004738-vnz15x84	75	3	denote	denote	VBP
cord-004738-vnz15x84	75	4	position	position	NN
cord-004738-vnz15x84	75	5	of	of	IN
cord-004738-vnz15x84	75	6	amino	amino	JJ
cord-004738-vnz15x84	75	7	acid	acid	NN
cord-004738-vnz15x84	75	8	residues	residue	NNS
cord-004738-vnz15x84	75	9	within	within	IN
cord-004738-vnz15x84	75	10	the	the	DT
cord-004738-vnz15x84	75	11	P200	P200	NNP
cord-004738-vnz15x84	75	12	polyprotein	polyprotein	NN
cord-004738-vnz15x84	75	13	representing	represent	VBG
cord-004738-vnz15x84	75	14	the	the	DT
cord-004738-vnz15x84	75	15	N	N	NNP
cord-004738-vnz15x84	75	16	and	and	CC
cord-004738-vnz15x84	75	17	C	C	NNP
cord-004738-vnz15x84	75	18	termini	terminus	NNS
cord-004738-vnz15x84	75	19	of	of	IN
cord-004738-vnz15x84	75	20	the	the	DT
cord-004738-vnz15x84	75	21	substrate	substrate	NN
cord-004738-vnz15x84	75	22	)	)	-RRB-
cord-004738-vnz15x84	75	23	used	use	VBN
cord-004738-vnz15x84	75	24	in	in	IN
cord-004738-vnz15x84	75	25	the	the	DT
cord-004738-vnz15x84	75	26	study	study	NN
cord-004738-vnz15x84	75	27	were	be	VBD
cord-004738-vnz15x84	75	28	as	as	RB
cord-004738-vnz15x84	75	29	illustrated	illustrate	VBN
cord-004738-vnz15x84	75	30	.	.	.
cord-004738-vnz15x84	76	1	These	these	DT
cord-004738-vnz15x84	76	2	plasmids	plasmid	NNS
cord-004738-vnz15x84	76	3	express	express	VBP
cord-004738-vnz15x84	76	4	proteins	protein	NNS
cord-004738-vnz15x84	76	5	with	with	IN
cord-004738-vnz15x84	76	6	varying	vary	VBG
cord-004738-vnz15x84	76	7	N	n	CD
cord-004738-vnz15x84	76	8	-	-	HYPH
cord-004738-vnz15x84	76	9	terminal	terminal	NN
cord-004738-vnz15x84	76	10	lengths	length	NNS
cord-004738-vnz15x84	76	11	from	from	IN
cord-004738-vnz15x84	76	12	the	the	DT
cord-004738-vnz15x84	76	13	cleavage	cleavage	NN
cord-004738-vnz15x84	76	14	site	site	NN
cord-004738-vnz15x84	76	15	(	(	-LRB-
cord-004738-vnz15x84	76	16	475	475	CD
cord-004738-vnz15x84	76	17	,	,	,
cord-004738-vnz15x84	76	18	309	309	CD
cord-004738-vnz15x84	76	19	,	,	,
cord-004738-vnz15x84	76	20	199	199	CD
cord-004738-vnz15x84	76	21	and	and	CC
cord-004738-vnz15x84	76	22	72	72	CD
cord-004738-vnz15x84	76	23	aa	aa	NN
cord-004738-vnz15x84	76	24	respectively	respectively	RB
cord-004738-vnz15x84	76	25	as	as	IN
cord-004738-vnz15x84	76	26	shown	show	VBN
cord-004738-vnz15x84	76	27	in	in	IN
cord-004738-vnz15x84	76	28	parentheses	parenthesis	NNS
cord-004738-vnz15x84	76	29	under	under	IN
cord-004738-vnz15x84	76	30	each	each	DT
cord-004738-vnz15x84	76	31	construct	construct	NN
cord-004738-vnz15x84	76	32	box	box	NN
cord-004738-vnz15x84	76	33	)	)	-RRB-
cord-004738-vnz15x84	76	34	.	.	.
cord-004738-vnz15x84	77	1	Cleavage	cleavage	NN
cord-004738-vnz15x84	77	2	in	in	IN
cord-004738-vnz15x84	77	3	the	the	DT
cord-004738-vnz15x84	77	4	substrate	substrate	NN
cord-004738-vnz15x84	77	5	occurs	occur	VBZ
cord-004738-vnz15x84	77	6	at	at	IN
cord-004738-vnz15x84	77	7	amino	amino	NN
cord-004738-vnz15x84	77	8	acid	acid	NN
cord-004738-vnz15x84	77	9	residue	residue	NN
cord-004738-vnz15x84	77	10	G1301	G1301	NNP
cord-004738-vnz15x84	77	11	.	.	.
cord-004738-vnz15x84	78	1	In	in	IN
cord-004738-vnz15x84	78	2	pRVS	prvs	JJ
cord-004738-vnz15x84	78	3	-	-	HYPH
cord-004738-vnz15x84	78	4	GFP	gfp	NN
cord-004738-vnz15x84	78	5	,	,	,
cord-004738-vnz15x84	78	6	the	the	DT
cord-004738-vnz15x84	78	7	RV	RV	NNP
cord-004738-vnz15x84	78	8	cDNA	cdna	NN
cord-004738-vnz15x84	78	9	insert	insert	NN
cord-004738-vnz15x84	78	10	was	be	VBD
cord-004738-vnz15x84	78	11	amplified	amplify	VBN
cord-004738-vnz15x84	78	12	from	from	IN
cord-004738-vnz15x84	78	13	position	position	NN
cord-004738-vnz15x84	78	14	827	827	CD
cord-004738-vnz15x84	78	15	-	-	HYPH
cord-004738-vnz15x84	78	16	1306	1306	CD
cord-004738-vnz15x84	79	1	(	(	-LRB-
cord-004738-vnz15x84	79	2	5	5	CD
cord-004738-vnz15x84	79	3	residues	residue	NNS
cord-004738-vnz15x84	79	4	upstream	upstream	RB
cord-004738-vnz15x84	79	5	of	of	IN
cord-004738-vnz15x84	79	6	the	the	DT
cord-004738-vnz15x84	79	7	cleavage	cleavage	NN
cord-004738-vnz15x84	79	8	site	site	NN
cord-004738-vnz15x84	79	9	)	)	-RRB-
cord-004738-vnz15x84	79	10	and	and	CC
cord-004738-vnz15x84	79	11	a	a	DT
cord-004738-vnz15x84	79	12	GFP	GFP	NNP
cord-004738-vnz15x84	79	13	-	-	HYPH
cord-004738-vnz15x84	79	14	ORF	ORF	NNP
cord-004738-vnz15x84	79	15	was	be	VBD
cord-004738-vnz15x84	79	16	fused	fuse	VBN
cord-004738-vnz15x84	79	17	at	at	IN
cord-004738-vnz15x84	79	18	its	-PRON-	PRP$
cord-004738-vnz15x84	79	19	C	c	NN
cord-004738-vnz15x84	79	20	-	-	HYPH
cord-004738-vnz15x84	79	21	terminus	terminus	NN
cord-004738-vnz15x84	79	22	,	,	,
cord-004738-vnz15x84	79	23	whereas	whereas	IN
cord-004738-vnz15x84	79	24	in	in	IN
cord-004738-vnz15x84	79	25	pRVS	prvs	JJ
cord-004738-vnz15x84	79	26	-	-	HYPH
cord-004738-vnz15x84	79	27	GFP-1102	gfp-1102	NN
cord-004738-vnz15x84	79	28	-	-	HYPH
cord-004738-vnz15x84	79	29	1548	1548	CD
cord-004738-vnz15x84	79	30	,	,	,
cord-004738-vnz15x84	79	31	the	the	DT
cord-004738-vnz15x84	79	32	GFP	GFP	NNP
cord-004738-vnz15x84	79	33	-	-	HYPH
cord-004738-vnz15x84	79	34	ORF	ORF	NNP
cord-004738-vnz15x84	79	35	was	be	VBD
cord-004738-vnz15x84	79	36	fused	fuse	VBN
cord-004738-vnz15x84	79	37	at	at	IN
cord-004738-vnz15x84	79	38	the	the	DT
cord-004738-vnz15x84	79	39	N	n	CD
cord-004738-vnz15x84	79	40	-	-	HYPH
cord-004738-vnz15x84	79	41	terminus	terminus	NN
cord-004738-vnz15x84	79	42	of	of	IN
cord-004738-vnz15x84	79	43	the	the	DT
cord-004738-vnz15x84	79	44	199	199	CD
cord-004738-vnz15x84	79	45	amino	amino	NN
cord-004738-vnz15x84	79	46	acid	acid	NN
cord-004738-vnz15x84	79	47	residue	residue	NN
cord-004738-vnz15x84	79	48	-	-	HYPH
cord-004738-vnz15x84	79	49	substrate	substrate	NN
cord-004738-vnz15x84	79	50	.	.	.
cord-004738-vnz15x84	80	1	All	all	DT
cord-004738-vnz15x84	80	2	plasmids	plasmid	NNS
cord-004738-vnz15x84	80	3	shown	show	VBN
cord-004738-vnz15x84	80	4	here	here	RB
cord-004738-vnz15x84	80	5	contain	contain	VBP
cord-004738-vnz15x84	80	6	an	an	DT
cord-004738-vnz15x84	80	7	in	in	IN
cord-004738-vnz15x84	80	8	-	-	HYPH
cord-004738-vnz15x84	80	9	frame	frame	NN
cord-004738-vnz15x84	80	10	N	n	CD
cord-004738-vnz15x84	80	11	-	-	HYPH
cord-004738-vnz15x84	80	12	terminal	terminal	JJ
cord-004738-vnz15x84	80	13	Xpress	Xpress	NNP
cord-004738-vnz15x84	80	14	epitope	epitope	NN
cord-004738-vnz15x84	80	15	tag	tag	NN
cord-004738-vnz15x84	80	16	(	(	-LRB-
cord-004738-vnz15x84	80	17	hatched	hatch	VBN
cord-004738-vnz15x84	80	18	box	box	NN
cord-004738-vnz15x84	80	19	)	)	-RRB-
cord-004738-vnz15x84	80	20	for	for	IN
cord-004738-vnz15x84	80	21	immunoblot	immunoblot	NNP
cord-004738-vnz15x84	80	22	identification	identification	NN
cord-004738-vnz15x84	80	23	of	of	IN
cord-004738-vnz15x84	80	24	the	the	DT
cord-004738-vnz15x84	80	25	proteins	protein	NNS
cord-004738-vnz15x84	80	26	 	 	_SP
cord-004738-vnz15x84	80	27	RV	rv	NN
cord-004738-vnz15x84	80	28	-	-	HYPH
cord-004738-vnz15x84	80	29	protease	protease	NN
cord-004738-vnz15x84	80	30	,	,	,
cord-004738-vnz15x84	80	31	and	and	CC
cord-004738-vnz15x84	80	32	substrate	substrate	NN
cord-004738-vnz15x84	80	33	plasmids	plasmid	NNS
cord-004738-vnz15x84	80	34	were	be	VBD
cord-004738-vnz15x84	80	35	expressed	express	VBN
cord-004738-vnz15x84	80	36	in	in	IN
cord-004738-vnz15x84	80	37	human	human	JJ
cord-004738-vnz15x84	80	38	embryonic	embryonic	JJ
cord-004738-vnz15x84	80	39	kidney	kidney	NN
cord-004738-vnz15x84	80	40	(	(	-LRB-
cord-004738-vnz15x84	80	41	HEK	HEK	NNP
cord-004738-vnz15x84	80	42	)	)	-RRB-
cord-004738-vnz15x84	80	43	293	293	CD
cord-004738-vnz15x84	80	44	T	t	NN
cord-004738-vnz15x84	80	45	cells	cell	NNS
cord-004738-vnz15x84	80	46	(	(	-LRB-
cord-004738-vnz15x84	80	47	ATCC	ATCC	NNP
cord-004738-vnz15x84	80	48	,	,	,
cord-004738-vnz15x84	80	49	VA	VA	NNP
cord-004738-vnz15x84	80	50	)	)	-RRB-
cord-004738-vnz15x84	80	51	by	by	IN
cord-004738-vnz15x84	80	52	transient	transient	JJ
cord-004738-vnz15x84	80	53	transfection	transfection	NN
cord-004738-vnz15x84	80	54	using	use	VBG
cord-004738-vnz15x84	80	55	Lipofectamine-2000	Lipofectamine-2000	NNP
cord-004738-vnz15x84	80	56	reagent	reagent	NN
cord-004738-vnz15x84	80	57	as	as	IN
cord-004738-vnz15x84	80	58	per	per	IN
cord-004738-vnz15x84	80	59	supplier	supplier	NN
cord-004738-vnz15x84	80	60	's	's	POS
cord-004738-vnz15x84	80	61	protocol	protocol	NN
cord-004738-vnz15x84	80	62	(	(	-LRB-
cord-004738-vnz15x84	80	63	Invitrogen	Invitrogen	NNP
cord-004738-vnz15x84	80	64	,	,	,
cord-004738-vnz15x84	80	65	CA	CA	NNP
cord-004738-vnz15x84	80	66	)	)	-RRB-
cord-004738-vnz15x84	80	67	.	.	.
cord-004738-vnz15x84	81	1	Following	follow	VBG
cord-004738-vnz15x84	81	2	a	a	DT
cord-004738-vnz15x84	81	3	24	24	CD
cord-004738-vnz15x84	81	4	h	h	NN
cord-004738-vnz15x84	81	5	incubation	incubation	NN
cord-004738-vnz15x84	81	6	of	of	IN
cord-004738-vnz15x84	81	7	cells	cell	NNS
cord-004738-vnz15x84	81	8	at	at	IN
cord-004738-vnz15x84	81	9	37	37	CD
cord-004738-vnz15x84	81	10	•	•	NN
cord-004738-vnz15x84	81	11	C	c	NN
cord-004738-vnz15x84	81	12	in	in	IN
cord-004738-vnz15x84	81	13	a	a	DT
cord-004738-vnz15x84	81	14	CO	co	NN
cord-004738-vnz15x84	81	15	2	2	CD
cord-004738-vnz15x84	81	16	(	(	-LRB-
cord-004738-vnz15x84	81	17	5	5	CD
cord-004738-vnz15x84	81	18	%	%	NN
cord-004738-vnz15x84	81	19	)	)	-RRB-
cord-004738-vnz15x84	81	20	incubator	incubator	NN
cord-004738-vnz15x84	81	21	,	,	,
cord-004738-vnz15x84	81	22	cell	cell	NN
cord-004738-vnz15x84	81	23	lysates	lysate	NNS
cord-004738-vnz15x84	81	24	were	be	VBD
cord-004738-vnz15x84	81	25	prepared	prepare	VBN
cord-004738-vnz15x84	81	26	and	and	CC
cord-004738-vnz15x84	81	27	subjected	subject	VBN
cord-004738-vnz15x84	81	28	to	to	IN
cord-004738-vnz15x84	81	29	polyacrylamide	polyacrylamide	NN
cord-004738-vnz15x84	81	30	gel	gel	NN
cord-004738-vnz15x84	81	31	electrophoresis	electrophoresis	NN
cord-004738-vnz15x84	81	32	(	(	-LRB-
cord-004738-vnz15x84	81	33	either	either	CC
cord-004738-vnz15x84	81	34	10	10	CD
cord-004738-vnz15x84	81	35	%	%	NN
cord-004738-vnz15x84	81	36	or	or	CC
cord-004738-vnz15x84	81	37	,	,	,
cord-004738-vnz15x84	81	38	4	4	CD
cord-004738-vnz15x84	81	39	-	-	SYM
cord-004738-vnz15x84	81	40	20	20	CD
cord-004738-vnz15x84	81	41	%	%	NN
cord-004738-vnz15x84	81	42	gradient	gradient	NN
cord-004738-vnz15x84	81	43	)	)	-RRB-
cord-004738-vnz15x84	81	44	with	with	IN
cord-004738-vnz15x84	81	45	0.1	0.1	CD
cord-004738-vnz15x84	81	46	%	%	NN
cord-004738-vnz15x84	81	47	sodium	sodium	NN
cord-004738-vnz15x84	81	48	dodecyl	dodecyl	NNP
cord-004738-vnz15x84	81	49	sulphate	sulphate	NN
cord-004738-vnz15x84	81	50	,	,	,
cord-004738-vnz15x84	81	51	followed	follow	VBN
cord-004738-vnz15x84	81	52	by	by	IN
cord-004738-vnz15x84	81	53	immunoblot	immunoblot	NNP
cord-004738-vnz15x84	81	54	analysis	analysis	NN
cord-004738-vnz15x84	81	55	as	as	IN
cord-004738-vnz15x84	81	56	described	describe	VBN
cord-004738-vnz15x84	81	57	[	[	-LRB-
cord-004738-vnz15x84	81	58	12	12	CD
cord-004738-vnz15x84	81	59	]	]	-RRB-
cord-004738-vnz15x84	81	60	.	.	.
cord-004738-vnz15x84	82	1	Substrates	substrate	NNS
cord-004738-vnz15x84	82	2	as	as	RB
cord-004738-vnz15x84	82	3	well	well	RB
cord-004738-vnz15x84	82	4	as	as	IN
cord-004738-vnz15x84	82	5	the	the	DT
cord-004738-vnz15x84	82	6	protease	protease	NN
cord-004738-vnz15x84	82	7	all	all	DT
cord-004738-vnz15x84	82	8	had	have	VBD
cord-004738-vnz15x84	82	9	an	an	DT
cord-004738-vnz15x84	82	10	Xpress	Xpress	NNP
cord-004738-vnz15x84	82	11	epitope	epitope	NN
cord-004738-vnz15x84	82	12	tag	tag	NN
cord-004738-vnz15x84	82	13	at	at	IN
cord-004738-vnz15x84	82	14	their	-PRON-	PRP$
cord-004738-vnz15x84	82	15	N	n	CD
cord-004738-vnz15x84	82	16	-	-	HYPH
cord-004738-vnz15x84	82	17	terminus	terminus	NN
cord-004738-vnz15x84	82	18	to	to	TO
cord-004738-vnz15x84	82	19	detect	detect	VB
cord-004738-vnz15x84	82	20	the	the	DT
cord-004738-vnz15x84	82	21	N	n	CD
cord-004738-vnz15x84	82	22	-	-	HYPH
cord-004738-vnz15x84	82	23	terminal	terminal	JJ
cord-004738-vnz15x84	82	24	side	side	NN
cord-004738-vnz15x84	82	25	of	of	IN
cord-004738-vnz15x84	82	26	the	the	DT
cord-004738-vnz15x84	82	27	cleavage	cleavage	NN
cord-004738-vnz15x84	82	28	products	product	NNS
cord-004738-vnz15x84	82	29	.	.	.
cord-004738-vnz15x84	83	1	We	-PRON-	PRP
cord-004738-vnz15x84	83	2	evaluated	evaluate	VBD
cord-004738-vnz15x84	83	3	the	the	DT
cord-004738-vnz15x84	83	4	protease	protease	NN
cord-004738-vnz15x84	83	5	trans	trans	JJ
cord-004738-vnz15x84	83	6	-	-	JJ
cord-004738-vnz15x84	83	7	cleavage	cleavage	JJ
cord-004738-vnz15x84	83	8	activity	activity	NN
cord-004738-vnz15x84	83	9	on	on	IN
cord-004738-vnz15x84	83	10	a	a	DT
cord-004738-vnz15x84	83	11	series	series	NN
cord-004738-vnz15x84	83	12	of	of	IN
cord-004738-vnz15x84	83	13	RV	RV	NNP
cord-004738-vnz15x84	83	14	P200-derived	p200-derived	JJ
cord-004738-vnz15x84	83	15	polypeptides	polypeptide	NNS
cord-004738-vnz15x84	83	16	in	in	IN
cord-004738-vnz15x84	83	17	which	which	WDT
cord-004738-vnz15x84	83	18	the	the	DT
cord-004738-vnz15x84	83	19	protease	protease	NN
cord-004738-vnz15x84	83	20	catalytic	catalytic	JJ
cord-004738-vnz15x84	83	21	site	site	NN
cord-004738-vnz15x84	83	22	at	at	IN
cord-004738-vnz15x84	83	23	amino	amino	NN
cord-004738-vnz15x84	83	24	acid	acid	NN
cord-004738-vnz15x84	83	25	position	position	NN
cord-004738-vnz15x84	83	26	1152	1152	CD
cord-004738-vnz15x84	83	27	was	be	VBD
cord-004738-vnz15x84	83	28	mutated	mutate	VBN
cord-004738-vnz15x84	83	29	(	(	-LRB-
cord-004738-vnz15x84	83	30	C1152S	C1152S	NNP
cord-004738-vnz15x84	83	31	)	)	-RRB-
cord-004738-vnz15x84	83	32	to	to	TO
cord-004738-vnz15x84	83	33	serve	serve	VB
cord-004738-vnz15x84	83	34	as	as	IN
cord-004738-vnz15x84	83	35	substrate	substrate	NN
cord-004738-vnz15x84	83	36	(	(	-LRB-
cord-004738-vnz15x84	83	37	i.e.	i.e.	FW
cord-004738-vnz15x84	83	38	to	to	IN
cord-004738-vnz15x84	83	39	lack	lack	JJ
cord-004738-vnz15x84	83	40	protease	protease	NN
cord-004738-vnz15x84	83	41	activity	activity	NN
cord-004738-vnz15x84	83	42	,	,	,
cord-004738-vnz15x84	83	43	but	but	CC
cord-004738-vnz15x84	83	44	retain	retain	VB
cord-004738-vnz15x84	83	45	the	the	DT
cord-004738-vnz15x84	83	46	cleavage	cleavage	NN
cord-004738-vnz15x84	83	47	site	site	NN
cord-004738-vnz15x84	83	48	)	)	-RRB-
cord-004738-vnz15x84	83	49	.	.	.
cord-004738-vnz15x84	84	1	HEK	HEK	NNP
cord-004738-vnz15x84	84	2	293	293	CD
cord-004738-vnz15x84	84	3	T	T	NNP
cord-004738-vnz15x84	84	4	cells	cell	NNS
cord-004738-vnz15x84	84	5	were	be	VBD
cord-004738-vnz15x84	84	6	co	co	JJ
cord-004738-vnz15x84	84	7	-	-	VBN
cord-004738-vnz15x84	84	8	transfected	transfecte	VBN
cord-004738-vnz15x84	84	9	with	with	IN
cord-004738-vnz15x84	84	10	pRVP	pRVP	NNS
cord-004738-vnz15x84	84	11	(	(	-LRB-
cord-004738-vnz15x84	84	12	Protease	Protease	NNP
cord-004738-vnz15x84	84	13	,	,	,
cord-004738-vnz15x84	84	14	Fig	Fig	NNP
cord-004738-vnz15x84	84	15	.	.	.
cord-004738-vnz15x84	84	16	1A	1a	NN
cord-004738-vnz15x84	84	17	)	)	-RRB-
cord-004738-vnz15x84	84	18	,	,	,
cord-004738-vnz15x84	84	19	and	and	CC
cord-004738-vnz15x84	84	20	each	each	DT
cord-004738-vnz15x84	84	21	of	of	IN
cord-004738-vnz15x84	84	22	the	the	DT
cord-004738-vnz15x84	84	23	substrate	substrate	JJ
cord-004738-vnz15x84	84	24	expression	expression	NN
cord-004738-vnz15x84	84	25	plasmids	plasmid	NNS
cord-004738-vnz15x84	84	26	,	,	,
cord-004738-vnz15x84	84	27	pRVS-827	pRVS-827	NNS
cord-004738-vnz15x84	84	28	-	-	HYPH
cord-004738-vnz15x84	84	29	1548	1548	CD
cord-004738-vnz15x84	84	30	,	,	,
cord-004738-vnz15x84	84	31	pRVS-994	prvs-994	CD
cord-004738-vnz15x84	84	32	-	-	SYM
cord-004738-vnz15x84	84	33	1548	1548	CD
cord-004738-vnz15x84	84	34	,	,	,
cord-004738-vnz15x84	84	35	pRVS-1102	pRVS-1102	NNS
cord-004738-vnz15x84	84	36	-	-	HYPH
cord-004738-vnz15x84	84	37	1548	1548	CD
cord-004738-vnz15x84	84	38	,	,	,
cord-004738-vnz15x84	84	39	and	and	CC
cord-004738-vnz15x84	84	40	pRVS-1228	prvs-1228	NN
cord-004738-vnz15x84	84	41	-	-	HYPH
cord-004738-vnz15x84	84	42	1548	1548	CD
cord-004738-vnz15x84	84	43	.	.	.
cord-004738-vnz15x84	85	1	Each	each	DT
cord-004738-vnz15x84	85	2	substrate	substrate	NN
cord-004738-vnz15x84	85	3	plasmid	plasmid	NN
cord-004738-vnz15x84	85	4	expressed	express	VBD
cord-004738-vnz15x84	85	5	RV	RV	NNP
cord-004738-vnz15x84	85	6	P200-derived	p200-derived	JJ
cord-004738-vnz15x84	85	7	polypeptides	polypeptide	NNS
cord-004738-vnz15x84	85	8	of	of	IN
cord-004738-vnz15x84	85	9	different	different	JJ
cord-004738-vnz15x84	85	10	N	n	CD
cord-004738-vnz15x84	85	11	-	-	HYPH
cord-004738-vnz15x84	85	12	terminal	terminal	NN
cord-004738-vnz15x84	85	13	lengths	length	NNS
cord-004738-vnz15x84	85	14	(	(	-LRB-
cord-004738-vnz15x84	85	15	72	72	CD
cord-004738-vnz15x84	85	16	-	-	SYM
cord-004738-vnz15x84	85	17	475	475	CD
cord-004738-vnz15x84	85	18	residues	residue	NNS
cord-004738-vnz15x84	85	19	)	)	-RRB-
cord-004738-vnz15x84	85	20	from	from	IN
cord-004738-vnz15x84	85	21	the	the	DT
cord-004738-vnz15x84	85	22	cleavage	cleavage	NN
cord-004738-vnz15x84	85	23	site	site	NN
cord-004738-vnz15x84	85	24	(	(	-LRB-
cord-004738-vnz15x84	85	25	Fig	Fig	NNP
cord-004738-vnz15x84	85	26	.	.	.
cord-004738-vnz15x84	85	27	1B	1b	CD
cord-004738-vnz15x84	85	28	)	)	-RRB-
cord-004738-vnz15x84	85	29	.	.	.
cord-004738-vnz15x84	86	1	Following	follow	VBG
cord-004738-vnz15x84	86	2	transfection	transfection	NN
cord-004738-vnz15x84	86	3	,	,	,
cord-004738-vnz15x84	86	4	cell	cell	NN
cord-004738-vnz15x84	86	5	lysates	lysate	NNS
cord-004738-vnz15x84	86	6	were	be	VBD
cord-004738-vnz15x84	86	7	prepared	prepare	VBN
cord-004738-vnz15x84	86	8	,	,	,
cord-004738-vnz15x84	86	9	and	and	CC
cord-004738-vnz15x84	86	10	the	the	DT
cord-004738-vnz15x84	86	11	proteins	protein	NNS
cord-004738-vnz15x84	86	12	were	be	VBD
cord-004738-vnz15x84	86	13	subjected	subject	VBN
cord-004738-vnz15x84	86	14	to	to	IN
cord-004738-vnz15x84	86	15	immunoblot	immunoblot	NNP
cord-004738-vnz15x84	86	16	analysis	analysis	NN
cord-004738-vnz15x84	86	17	.	.	.
cord-004738-vnz15x84	87	1	The	the	DT
cord-004738-vnz15x84	87	2	substrates	substrate	NNS
cord-004738-vnz15x84	87	3	as	as	RB
cord-004738-vnz15x84	87	4	well	well	RB
cord-004738-vnz15x84	87	5	as	as	IN
cord-004738-vnz15x84	87	6	the	the	DT
cord-004738-vnz15x84	87	7	protease	protease	NN
cord-004738-vnz15x84	87	8	all	all	DT
cord-004738-vnz15x84	87	9	had	have	VBD
cord-004738-vnz15x84	87	10	an	an	DT
cord-004738-vnz15x84	87	11	Xpress	Xpress	NNP
cord-004738-vnz15x84	87	12	epitope	epitope	NN
cord-004738-vnz15x84	87	13	tag	tag	NN
cord-004738-vnz15x84	87	14	at	at	IN
cord-004738-vnz15x84	87	15	their	-PRON-	PRP$
cord-004738-vnz15x84	87	16	N	n	CD
cord-004738-vnz15x84	87	17	-	-	HYPH
cord-004738-vnz15x84	87	18	terminus	terminus	NN
cord-004738-vnz15x84	87	19	.	.	.
cord-004738-vnz15x84	88	1	Our	-PRON-	PRP$
cord-004738-vnz15x84	88	2	analysis	analysis	NN
cord-004738-vnz15x84	88	3	of	of	IN
cord-004738-vnz15x84	88	4	RV	rv	NN
cord-004738-vnz15x84	88	5	protease	protease	NN
cord-004738-vnz15x84	88	6	substrates	substrate	NNS
cord-004738-vnz15x84	88	7	revealed	reveal	VBD
cord-004738-vnz15x84	88	8	that	that	IN
cord-004738-vnz15x84	88	9	homologous	homologous	JJ
cord-004738-vnz15x84	88	10	polypeptides	polypeptide	NNS
cord-004738-vnz15x84	88	11	of	of	IN
cord-004738-vnz15x84	88	12	N	n	CD
cord-004738-vnz15x84	88	13	-	-	HYPH
cord-004738-vnz15x84	88	14	terminal	terminal	NN
cord-004738-vnz15x84	88	15	475-and	475-and	CD
cord-004738-vnz15x84	88	16	309-residue	309-residue	CD
cord-004738-vnz15x84	88	17	length	length	NN
cord-004738-vnz15x84	88	18	from	from	IN
cord-004738-vnz15x84	88	19	the	the	DT
cord-004738-vnz15x84	88	20	cleavage	cleavage	NN
cord-004738-vnz15x84	88	21	site	site	NN
cord-004738-vnz15x84	88	22	,	,	,
cord-004738-vnz15x84	88	23	expressed	express	VBN
cord-004738-vnz15x84	88	24	from	from	IN
cord-004738-vnz15x84	88	25	pRVS-827	pRVS-827	NNS
cord-004738-vnz15x84	88	26	-	-	HYPH
cord-004738-vnz15x84	88	27	1548	1548	CD
cord-004738-vnz15x84	88	28	and	and	CC
cord-004738-vnz15x84	88	29	pRVS-994	prvs-994	CD
cord-004738-vnz15x84	88	30	-	-	HYPH
cord-004738-vnz15x84	88	31	1548	1548	CD
cord-004738-vnz15x84	88	32	(	(	-LRB-
cord-004738-vnz15x84	88	33	Fig	Fig	NNP
cord-004738-vnz15x84	88	34	.	.	NNP
cord-004738-vnz15x84	88	35	2	2	CD
cord-004738-vnz15x84	88	36	,	,	,
cord-004738-vnz15x84	88	37	lanes	lane	NNS
cord-004738-vnz15x84	88	38	2	2	CD
cord-004738-vnz15x84	88	39	and	and	CC
cord-004738-vnz15x84	88	40	4	4	CD
cord-004738-vnz15x84	88	41	,	,	,
cord-004738-vnz15x84	88	42	80	80	CD
cord-004738-vnz15x84	88	43	and	and	CC
cord-004738-vnz15x84	88	44	67	67	CD
cord-004738-vnz15x84	88	45	kDa	kDa	NNS
cord-004738-vnz15x84	88	46	,	,	,
cord-004738-vnz15x84	88	47	respectively	respectively	RB
cord-004738-vnz15x84	88	48	)	)	-RRB-
cord-004738-vnz15x84	88	49	,	,	,
cord-004738-vnz15x84	88	50	were	be	VBD
cord-004738-vnz15x84	88	51	able	able	JJ
cord-004738-vnz15x84	88	52	to	to	TO
cord-004738-vnz15x84	88	53	undergo	undergo	VB
cord-004738-vnz15x84	88	54	cleavage	cleavage	NN
cord-004738-vnz15x84	88	55	when	when	WRB
cord-004738-vnz15x84	88	56	co	co	NN
cord-004738-vnz15x84	88	57	-	-	VBN
cord-004738-vnz15x84	88	58	expressed	express	VBN
cord-004738-vnz15x84	88	59	along	along	IN
cord-004738-vnz15x84	88	60	with	with	IN
cord-004738-vnz15x84	88	61	RV	rv	NN
cord-004738-vnz15x84	88	62	protease	protease	NN
cord-004738-vnz15x84	88	63	(	(	-LRB-
cord-004738-vnz15x84	88	64	Fig	Fig	NNP
cord-004738-vnz15x84	88	65	.	.	NNP
cord-004738-vnz15x84	88	66	2	2	CD
cord-004738-vnz15x84	88	67	,	,	,
cord-004738-vnz15x84	88	68	lanes	lane	NNS
cord-004738-vnz15x84	88	69	1	1	CD
cord-004738-vnz15x84	88	70	,	,	,
cord-004738-vnz15x84	88	71	3	3	CD
cord-004738-vnz15x84	88	72	,	,	,
cord-004738-vnz15x84	88	73	and	and	CC
cord-004738-vnz15x84	88	74	5	5	CD
cord-004738-vnz15x84	88	75	,	,	,
cord-004738-vnz15x84	88	76	110	110	CD
cord-004738-vnz15x84	88	77	kDa	kDa	NNS
cord-004738-vnz15x84	88	78	)	)	-RRB-
cord-004738-vnz15x84	88	79	with	with	IN
cord-004738-vnz15x84	88	80	an	an	DT
cord-004738-vnz15x84	88	81	expected	expect	VBN
cord-004738-vnz15x84	88	82	size	size	NN
cord-004738-vnz15x84	88	83	of	of	IN
cord-004738-vnz15x84	88	84	the	the	DT
cord-004738-vnz15x84	88	85	N	n	CD
cord-004738-vnz15x84	88	86	-	-	HYPH
cord-004738-vnz15x84	88	87	terminal	terminal	NN
cord-004738-vnz15x84	88	88	cleaved	cleave	VBN
cord-004738-vnz15x84	88	89	products	product	NNS
cord-004738-vnz15x84	88	90	of	of	IN
cord-004738-vnz15x84	88	91	55-and	55-and	CD
cord-004738-vnz15x84	88	92	37-kDa	37-kda	CD
cord-004738-vnz15x84	88	93	size	size	NN
cord-004738-vnz15x84	88	94	(	(	-LRB-
cord-004738-vnz15x84	88	95	with	with	IN
cord-004738-vnz15x84	88	96	additional	additional	JJ
cord-004738-vnz15x84	88	97	Xpress	Xpress	NNP
cord-004738-vnz15x84	88	98	epitope	epitope	NN
cord-004738-vnz15x84	88	99	residues	residue	NNS
cord-004738-vnz15x84	88	100	)	)	-RRB-
cord-004738-vnz15x84	88	101	,	,	,
cord-004738-vnz15x84	88	102	respectively	respectively	RB
cord-004738-vnz15x84	88	103	(	(	-LRB-
cord-004738-vnz15x84	88	104	Fig	Fig	NNP
cord-004738-vnz15x84	88	105	.	.	NNP
cord-004738-vnz15x84	88	106	2	2	CD
cord-004738-vnz15x84	88	107	,	,	,
cord-004738-vnz15x84	88	108	lanes	lane	NNS
cord-004738-vnz15x84	88	109	3	3	CD
cord-004738-vnz15x84	88	110	and	and	CC
cord-004738-vnz15x84	88	111	5	5	CD
cord-004738-vnz15x84	88	112	)	)	-RRB-
cord-004738-vnz15x84	88	113	.	.	.
cord-004738-vnz15x84	89	1	However	however	RB
cord-004738-vnz15x84	89	2	,	,	,
cord-004738-vnz15x84	89	3	sub-	sub-	DT
cord-004738-vnz15x84	89	4	shown	show	VBN
cord-004738-vnz15x84	89	5	are	be	VBP
cord-004738-vnz15x84	89	6	longer	long	JJR
cord-004738-vnz15x84	89	7	exposures	exposure	NNS
cord-004738-vnz15x84	89	8	of	of	IN
cord-004738-vnz15x84	89	9	the	the	DT
cord-004738-vnz15x84	89	10	same	same	JJ
cord-004738-vnz15x84	89	11	blot	blot	NN
cord-004738-vnz15x84	89	12	to	to	TO
cord-004738-vnz15x84	89	13	film	film	VB
cord-004738-vnz15x84	89	14	.	.	.
cord-004738-vnz15x84	90	1	Note	note	VB
cord-004738-vnz15x84	90	2	that	that	IN
cord-004738-vnz15x84	90	3	substrates	substrate	NNS
cord-004738-vnz15x84	90	4	generated	generate	VBN
cord-004738-vnz15x84	90	5	from	from	IN
cord-004738-vnz15x84	90	6	pRVS-827	pRVS-827	NNS
cord-004738-vnz15x84	90	7	-	-	HYPH
cord-004738-vnz15x84	90	8	1548	1548	CD
cord-004738-vnz15x84	90	9	and	and	CC
cord-004738-vnz15x84	90	10	pRVS-994	prvs-994	CD
cord-004738-vnz15x84	90	11	-	-	HYPH
cord-004738-vnz15x84	90	12	1548	1548	CD
cord-004738-vnz15x84	90	13	were	be	VBD
cord-004738-vnz15x84	90	14	cleaved	cleave	VBN
cord-004738-vnz15x84	90	15	by	by	IN
cord-004738-vnz15x84	90	16	the	the	DT
cord-004738-vnz15x84	90	17	protease	protease	NN
cord-004738-vnz15x84	90	18	(	(	-LRB-
cord-004738-vnz15x84	90	19	"	"	``
cord-004738-vnz15x84	90	20	>	>	XX
cord-004738-vnz15x84	90	21	"	"	''
cord-004738-vnz15x84	90	22	identifies	identify	VBZ
cord-004738-vnz15x84	90	23	the	the	DT
cord-004738-vnz15x84	90	24	cleaved	cleaved	JJ
cord-004738-vnz15x84	90	25	products	product	NNS
cord-004738-vnz15x84	90	26	of	of	IN
cord-004738-vnz15x84	90	27	475	475	CD
cord-004738-vnz15x84	90	28	and	and	CC
cord-004738-vnz15x84	90	29	309	309	CD
cord-004738-vnz15x84	90	30	residues	residue	NNS
cord-004738-vnz15x84	90	31	respectively	respectively	RB
cord-004738-vnz15x84	90	32	in	in	IN
cord-004738-vnz15x84	90	33	3	3	CD
cord-004738-vnz15x84	90	34	and	and	CC
cord-004738-vnz15x84	90	35	5	5	CD
cord-004738-vnz15x84	90	36	)	)	-RRB-
cord-004738-vnz15x84	90	37	,	,	,
cord-004738-vnz15x84	90	38	whereas	whereas	RB
cord-004738-vnz15x84	90	39	,	,	,
cord-004738-vnz15x84	90	40	199-and	199-and	CD
cord-004738-vnz15x84	90	41	72-residue	72-residue	CD
cord-004738-vnz15x84	90	42	(	(	-LRB-
cord-004738-vnz15x84	90	43	from	from	IN
cord-004738-vnz15x84	90	44	the	the	DT
cord-004738-vnz15x84	90	45	cleavage	cleavage	NN
cord-004738-vnz15x84	90	46	site	site	NN
cord-004738-vnz15x84	90	47	)	)	-RRB-
cord-004738-vnz15x84	90	48	representing	represent	VBG
cord-004738-vnz15x84	90	49	substrates	substrate	NNS
cord-004738-vnz15x84	90	50	expressed	express	VBN
cord-004738-vnz15x84	90	51	from	from	IN
cord-004738-vnz15x84	90	52	pRVS-1102	pRVS-1102	NNS
cord-004738-vnz15x84	90	53	-	-	:
cord-004738-vnz15x84	90	54	1548	1548	CD
cord-004738-vnz15x84	90	55	and	and	CC
cord-004738-vnz15x84	90	56	pRVS-1228	pRVS-1228	NNS
cord-004738-vnz15x84	90	57	-	-	HYPH
cord-004738-vnz15x84	90	58	1548	1548	CD
cord-004738-vnz15x84	90	59	were	be	VBD
cord-004738-vnz15x84	90	60	not	not	RB
cord-004738-vnz15x84	90	61	cleaved	cleave	VBN
cord-004738-vnz15x84	90	62	by	by	IN
cord-004738-vnz15x84	90	63	the	the	DT
cord-004738-vnz15x84	90	64	protease	protease	NN
cord-004738-vnz15x84	90	65	(	(	-LRB-
cord-004738-vnz15x84	90	66	7	7	CD
cord-004738-vnz15x84	90	67	and	and	CC
cord-004738-vnz15x84	90	68	9	9	CD
cord-004738-vnz15x84	90	69	)	)	-RRB-
cord-004738-vnz15x84	90	70	.	.	.
cord-004738-vnz15x84	91	1	Also	also	RB
cord-004738-vnz15x84	91	2	note	note	VB
cord-004738-vnz15x84	91	3	that	that	IN
cord-004738-vnz15x84	91	4	the	the	DT
cord-004738-vnz15x84	91	5	protease	protease	NN
cord-004738-vnz15x84	91	6	was	be	VBD
cord-004738-vnz15x84	91	7	able	able	JJ
cord-004738-vnz15x84	91	8	to	to	TO
cord-004738-vnz15x84	91	9	cleave	cleave	VB
cord-004738-vnz15x84	91	10	the	the	DT
cord-004738-vnz15x84	91	11	RVS	RVS	NNP
cord-004738-vnz15x84	91	12	-	-	HYPH
cord-004738-vnz15x84	91	13	GFP	GFP	NNP
cord-004738-vnz15x84	91	14	substrate	substrate	NN
cord-004738-vnz15x84	91	15	with	with	IN
cord-004738-vnz15x84	91	16	a	a	DT
cord-004738-vnz15x84	91	17	GFP	GFP	NNP
cord-004738-vnz15x84	91	18	on	on	IN
cord-004738-vnz15x84	91	19	the	the	DT
cord-004738-vnz15x84	91	20	C	C	NNP
cord-004738-vnz15x84	91	21	-	-	HYPH
cord-004738-vnz15x84	91	22	terminal	terminal	NN
cord-004738-vnz15x84	91	23	side	side	NN
cord-004738-vnz15x84	91	24	of	of	IN
cord-004738-vnz15x84	91	25	the	the	DT
cord-004738-vnz15x84	91	26	cleavage	cleavage	NN
cord-004738-vnz15x84	91	27	site	site	NN
cord-004738-vnz15x84	91	28	to	to	IN
cord-004738-vnz15x84	91	29	a	a	DT
cord-004738-vnz15x84	91	30	lesser	less	JJR
cord-004738-vnz15x84	91	31	extent	extent	NN
cord-004738-vnz15x84	91	32	(	(	-LRB-
cord-004738-vnz15x84	91	33	<	<	XX
cord-004738-vnz15x84	91	34	50	50	CD
cord-004738-vnz15x84	91	35	%	%	NN
cord-004738-vnz15x84	91	36	)	)	-RRB-
cord-004738-vnz15x84	91	37	,	,	,
cord-004738-vnz15x84	91	38	as	as	IN
cord-004738-vnz15x84	91	39	a	a	DT
cord-004738-vnz15x84	91	40	significant	significant	JJ
cord-004738-vnz15x84	91	41	portion	portion	NN
cord-004738-vnz15x84	91	42	of	of	IN
cord-004738-vnz15x84	91	43	the	the	DT
cord-004738-vnz15x84	91	44	substrate	substrate	NN
cord-004738-vnz15x84	91	45	remained	remain	VBD
cord-004738-vnz15x84	91	46	uncleaved	uncleaved	JJ
cord-004738-vnz15x84	91	47	strates	strate	NNS
cord-004738-vnz15x84	91	48	of	of	IN
cord-004738-vnz15x84	91	49	N	n	CD
cord-004738-vnz15x84	91	50	-	-	HYPH
cord-004738-vnz15x84	91	51	terminal	terminal	NN
cord-004738-vnz15x84	91	52	199-and	199-and	CD
cord-004738-vnz15x84	91	53	72-residue	72-residue	CD
cord-004738-vnz15x84	91	54	length	length	NN
cord-004738-vnz15x84	91	55	from	from	IN
cord-004738-vnz15x84	91	56	the	the	DT
cord-004738-vnz15x84	91	57	cleavage	cleavage	NN
cord-004738-vnz15x84	91	58	site	site	NN
cord-004738-vnz15x84	91	59	(	(	-LRB-
cord-004738-vnz15x84	91	60	plus	plus	CC
cord-004738-vnz15x84	91	61	the	the	DT
cord-004738-vnz15x84	91	62	Xpress	Xpress	NNP
cord-004738-vnz15x84	91	63	epitope	epitope	NN
cord-004738-vnz15x84	91	64	residues	residue	NNS
cord-004738-vnz15x84	91	65	)	)	-RRB-
cord-004738-vnz15x84	91	66	,	,	,
cord-004738-vnz15x84	91	67	expressed	express	VBN
cord-004738-vnz15x84	91	68	from	from	IN
cord-004738-vnz15x84	91	69	pRVS-1102	pRVS-1102	NNS
cord-004738-vnz15x84	91	70	-	-	:
cord-004738-vnz15x84	91	71	1548	1548	CD
cord-004738-vnz15x84	91	72	and	and	CC
cord-004738-vnz15x84	91	73	pRVS-1228	pRVS-1228	NNS
cord-004738-vnz15x84	91	74	-	-	HYPH
cord-004738-vnz15x84	91	75	1548	1548	CD
cord-004738-vnz15x84	91	76	,	,	,
cord-004738-vnz15x84	91	77	respectively	respectively	RB
cord-004738-vnz15x84	91	78	(	(	-LRB-
cord-004738-vnz15x84	91	79	Fig	Fig	NNP
cord-004738-vnz15x84	91	80	.	.	NNP
cord-004738-vnz15x84	91	81	2	2	CD
cord-004738-vnz15x84	91	82	,	,	,
cord-004738-vnz15x84	91	83	lanes	lane	NNS
cord-004738-vnz15x84	91	84	,	,	,
cord-004738-vnz15x84	91	85	6	6	CD
cord-004738-vnz15x84	91	86	and	and	CC
cord-004738-vnz15x84	91	87	8	8	CD
cord-004738-vnz15x84	91	88	,	,	,
cord-004738-vnz15x84	91	89	55-and	55-and	CD
cord-004738-vnz15x84	91	90	37-kDa	37-kda	CD
cord-004738-vnz15x84	91	91	)	)	-RRB-
cord-004738-vnz15x84	91	92	,	,	,
cord-004738-vnz15x84	91	93	did	do	VBD
cord-004738-vnz15x84	91	94	not	not	RB
cord-004738-vnz15x84	91	95	undergo	undergo	VB
cord-004738-vnz15x84	91	96	cleavage	cleavage	NN
cord-004738-vnz15x84	91	97	when	when	WRB
cord-004738-vnz15x84	91	98	co	co	NN
cord-004738-vnz15x84	91	99	-	-	VBN
cord-004738-vnz15x84	91	100	expressed	express	VBN
cord-004738-vnz15x84	91	101	along	along	IN
cord-004738-vnz15x84	91	102	with	with	IN
cord-004738-vnz15x84	91	103	the	the	DT
cord-004738-vnz15x84	91	104	protease	protease	NN
cord-004738-vnz15x84	91	105	(	(	-LRB-
cord-004738-vnz15x84	91	106	Fig	Fig	NNP
cord-004738-vnz15x84	91	107	.	.	NNP
cord-004738-vnz15x84	91	108	2	2	CD
cord-004738-vnz15x84	91	109	,	,	,
cord-004738-vnz15x84	91	110	lanes	lane	NNS
cord-004738-vnz15x84	91	111	7	7	CD
cord-004738-vnz15x84	91	112	and	and	CC
cord-004738-vnz15x84	91	113	9	9	CD
cord-004738-vnz15x84	91	114	)	)	-RRB-
cord-004738-vnz15x84	91	115	as	as	IN
cord-004738-vnz15x84	91	116	evidenced	evidence	VBN
cord-004738-vnz15x84	91	117	by	by	IN
cord-004738-vnz15x84	91	118	the	the	DT
cord-004738-vnz15x84	91	119	lack	lack	NN
cord-004738-vnz15x84	91	120	of	of	IN
cord-004738-vnz15x84	91	121	Xpress	Xpress	NNP
cord-004738-vnz15x84	91	122	-	-	HYPH
cord-004738-vnz15x84	91	123	tagged	tagged	NNP
cord-004738-vnz15x84	91	124	N	N	NNP
cord-004738-vnz15x84	91	125	-	-	HYPH
cord-004738-vnz15x84	91	126	terminal	terminal	NN
cord-004738-vnz15x84	91	127	cleavage	cleavage	NN
cord-004738-vnz15x84	91	128	products	product	NNS
cord-004738-vnz15x84	91	129	detected	detect	VBN
cord-004738-vnz15x84	91	130	in	in	IN
cord-004738-vnz15x84	91	131	the	the	DT
cord-004738-vnz15x84	91	132	immunoblot	immunoblot	NNP
cord-004738-vnz15x84	91	133	analysis	analysis	NN
cord-004738-vnz15x84	91	134	.	.	.
cord-004738-vnz15x84	92	1	(	(	-LRB-
cord-004738-vnz15x84	92	2	If	if	IN
cord-004738-vnz15x84	92	3	the	the	DT
cord-004738-vnz15x84	92	4	substrates	substrate	NNS
cord-004738-vnz15x84	92	5	were	be	VBD
cord-004738-vnz15x84	92	6	cleaved	cleave	VBN
cord-004738-vnz15x84	92	7	,	,	,
cord-004738-vnz15x84	92	8	the	the	DT
cord-004738-vnz15x84	92	9	expected	expect	VBN
cord-004738-vnz15x84	92	10	sizes	size	NNS
cord-004738-vnz15x84	92	11	of	of	IN
cord-004738-vnz15x84	92	12	the	the	DT
cord-004738-vnz15x84	92	13	N	n	CD
cord-004738-vnz15x84	92	14	-	-	HYPH
cord-004738-vnz15x84	92	15	terminal	terminal	NN
cord-004738-vnz15x84	92	16	products	product	NNS
cord-004738-vnz15x84	92	17	would	would	MD
cord-004738-vnz15x84	92	18	be	be	VB
cord-004738-vnz15x84	92	19	25	25	CD
cord-004738-vnz15x84	92	20	kDa	kDa	NNS
cord-004738-vnz15x84	92	21	and	and	CC
cord-004738-vnz15x84	92	22	14	14	CD
cord-004738-vnz15x84	92	23	kDa	kDa	NNS
cord-004738-vnz15x84	92	24	,	,	,
cord-004738-vnz15x84	92	25	respectively	respectively	RB
cord-004738-vnz15x84	92	26	.	.	.
cord-004738-vnz15x84	92	27	)	)	-RRB-
cord-004738-vnz15x84	93	1	This	this	DT
cord-004738-vnz15x84	93	2	clearly	clearly	RB
cord-004738-vnz15x84	93	3	suggests	suggest	VBZ
cord-004738-vnz15x84	93	4	that	that	IN
cord-004738-vnz15x84	93	5	amino	amino	JJ
cord-004738-vnz15x84	93	6	acid	acid	NN
cord-004738-vnz15x84	93	7	residues	residue	NNS
cord-004738-vnz15x84	93	8	residing	reside	VBG
cord-004738-vnz15x84	93	9	between	between	IN
cord-004738-vnz15x84	93	10	residues	residue	NNS
cord-004738-vnz15x84	93	11	200	200	CD
cord-004738-vnz15x84	93	12	-	-	SYM
cord-004738-vnz15x84	93	13	308	308	CD
cord-004738-vnz15x84	93	14	in	in	IN
cord-004738-vnz15x84	93	15	the	the	DT
cord-004738-vnz15x84	93	16	substrate	substrate	NN
cord-004738-vnz15x84	93	17	are	be	VBP
cord-004738-vnz15x84	93	18	required	require	VBN
cord-004738-vnz15x84	93	19	in	in	IN
cord-004738-vnz15x84	93	20	order	order	NN
cord-004738-vnz15x84	93	21	for	for	IN
cord-004738-vnz15x84	93	22	the	the	DT
cord-004738-vnz15x84	93	23	substrate	substrate	NN
cord-004738-vnz15x84	93	24	to	to	TO
cord-004738-vnz15x84	93	25	be	be	VB
cord-004738-vnz15x84	93	26	targeted	target	VBN
cord-004738-vnz15x84	93	27	by	by	IN
cord-004738-vnz15x84	93	28	the	the	DT
cord-004738-vnz15x84	93	29	trans	trans	NN
cord-004738-vnz15x84	93	30	-	-	NN
cord-004738-vnz15x84	93	31	activity	activity	NN
cord-004738-vnz15x84	93	32	of	of	IN
cord-004738-vnz15x84	93	33	the	the	DT
cord-004738-vnz15x84	93	34	protease	protease	NN
cord-004738-vnz15x84	93	35	.	.	.
cord-004738-vnz15x84	94	1	We	-PRON-	PRP
cord-004738-vnz15x84	94	2	extended	extend	VBD
cord-004738-vnz15x84	94	3	our	-PRON-	PRP$
cord-004738-vnz15x84	94	4	analysis	analysis	NN
cord-004738-vnz15x84	94	5	to	to	TO
cord-004738-vnz15x84	94	6	identify	identify	VB
cord-004738-vnz15x84	94	7	any	any	DT
cord-004738-vnz15x84	94	8	homologous	homologous	JJ
cord-004738-vnz15x84	94	9	sequence	sequence	NN
cord-004738-vnz15x84	94	10	requirement	requirement	NN
cord-004738-vnz15x84	94	11	of	of	IN
cord-004738-vnz15x84	94	12	the	the	DT
cord-004738-vnz15x84	94	13	substrate	substrate	NN
cord-004738-vnz15x84	94	14	on	on	IN
cord-004738-vnz15x84	94	15	the	the	DT
cord-004738-vnz15x84	94	16	C	C	NNP
cord-004738-vnz15x84	94	17	-	-	HYPH
cord-004738-vnz15x84	94	18	terminal	terminal	NN
cord-004738-vnz15x84	94	19	side	side	NN
cord-004738-vnz15x84	94	20	of	of	IN
cord-004738-vnz15x84	94	21	the	the	DT
cord-004738-vnz15x84	94	22	cleavage	cleavage	NN
cord-004738-vnz15x84	94	23	site	site	NN
cord-004738-vnz15x84	94	24	.	.	.
cord-004738-vnz15x84	95	1	To	to	TO
cord-004738-vnz15x84	95	2	address	address	VB
cord-004738-vnz15x84	95	3	this	this	DT
cord-004738-vnz15x84	95	4	,	,	,
cord-004738-vnz15x84	95	5	we	-PRON-	PRP
cord-004738-vnz15x84	95	6	utilized	utilize	VBD
cord-004738-vnz15x84	95	7	the	the	DT
cord-004738-vnz15x84	95	8	plasmid	plasmid	NN
cord-004738-vnz15x84	95	9	pRVS	prvs	JJ
cord-004738-vnz15x84	95	10	-	-	HYPH
cord-004738-vnz15x84	95	11	GFP	GFP	NNP
cord-004738-vnz15x84	95	12	,	,	,
cord-004738-vnz15x84	95	13	which	which	WDT
cord-004738-vnz15x84	95	14	expresses	express	VBZ
cord-004738-vnz15x84	95	15	an	an	DT
cord-004738-vnz15x84	95	16	RV	RV	NNP
cord-004738-vnz15x84	95	17	protease	protease	NN
cord-004738-vnz15x84	95	18	homologous	homologous	JJ
cord-004738-vnz15x84	95	19	substrate	substrate	NN
cord-004738-vnz15x84	95	20	representing	represent	VBG
cord-004738-vnz15x84	95	21	amino	amino	JJ
cord-004738-vnz15x84	95	22	acids	acid	NNS
cord-004738-vnz15x84	95	23	827	827	CD
cord-004738-vnz15x84	95	24	-	-	HYPH
cord-004738-vnz15x84	95	25	1306	1306	CD
cord-004738-vnz15x84	96	1	(	(	-LRB-
cord-004738-vnz15x84	96	2	residues	residue	NNS
cord-004738-vnz15x84	96	3	1302	1302	CD
cord-004738-vnz15x84	96	4	-	-	SYM
cord-004738-vnz15x84	96	5	1306	1306	CD
cord-004738-vnz15x84	96	6	represent	represent	VBP
cord-004738-vnz15x84	96	7	the	the	DT
cord-004738-vnz15x84	96	8	C	C	NNP
cord-004738-vnz15x84	96	9	-	-	HYPH
cord-004738-vnz15x84	96	10	terminal	terminal	NN
cord-004738-vnz15x84	96	11	side	side	NN
cord-004738-vnz15x84	96	12	of	of	IN
cord-004738-vnz15x84	96	13	the	the	DT
cord-004738-vnz15x84	96	14	cleavage	cleavage	NN
cord-004738-vnz15x84	96	15	site	site	NN
cord-004738-vnz15x84	96	16	)	)	-RRB-
cord-004738-vnz15x84	96	17	,	,	,
cord-004738-vnz15x84	96	18	fused	fuse	VBN
cord-004738-vnz15x84	96	19	at	at	IN
cord-004738-vnz15x84	96	20	its	-PRON-	PRP$
cord-004738-vnz15x84	96	21	C	c	NN
cord-004738-vnz15x84	96	22	-	-	HYPH
cord-004738-vnz15x84	96	23	terminus	terminus	NN
cord-004738-vnz15x84	96	24	to	to	IN
cord-004738-vnz15x84	96	25	a	a	DT
cord-004738-vnz15x84	96	26	GFP	GFP	NNP
cord-004738-vnz15x84	96	27	ORF	ORF	NNP
cord-004738-vnz15x84	96	28	in	in	IN
cord-004738-vnz15x84	96	29	-	-	HYPH
cord-004738-vnz15x84	96	30	frame	frame	NN
cord-004738-vnz15x84	96	31	(	(	-LRB-
cord-004738-vnz15x84	96	32	Fig	fig	NN
cord-004738-vnz15x84	96	33	.	.	.
cord-004738-vnz15x84	96	34	1B	1b	CD
cord-004738-vnz15x84	96	35	)	)	-RRB-
cord-004738-vnz15x84	96	36	.	.	.
cord-004738-vnz15x84	97	1	When	when	WRB
cord-004738-vnz15x84	97	2	this	this	DT
cord-004738-vnz15x84	97	3	plasmid	plasmid	NN
cord-004738-vnz15x84	97	4	was	be	VBD
cord-004738-vnz15x84	97	5	co	co	VBN
cord-004738-vnz15x84	97	6	-	-	VBN
cord-004738-vnz15x84	97	7	transfected	transfecte	VBN
cord-004738-vnz15x84	98	1	along	along	IN
cord-004738-vnz15x84	98	2	with	with	IN
cord-004738-vnz15x84	98	3	RV	rv	NN
cord-004738-vnz15x84	98	4	protease	protease	NN
cord-004738-vnz15x84	98	5	plasmid	plasmid	NN
cord-004738-vnz15x84	98	6	(	(	-LRB-
cord-004738-vnz15x84	98	7	pRVP	pRVP	NNP
cord-004738-vnz15x84	98	8	)	)	-RRB-
cord-004738-vnz15x84	98	9	into	into	IN
cord-004738-vnz15x84	98	10	HEK	HEK	NNP
cord-004738-vnz15x84	98	11	293	293	CD
cord-004738-vnz15x84	98	12	T	T	NNP
cord-004738-vnz15x84	98	13	cells	cell	NNS
cord-004738-vnz15x84	98	14	,	,	,
cord-004738-vnz15x84	98	15	we	-PRON-	PRP
cord-004738-vnz15x84	98	16	observed	observe	VBD
cord-004738-vnz15x84	98	17	that	that	IN
cord-004738-vnz15x84	98	18	the	the	DT
cord-004738-vnz15x84	98	19	substrate	substrate	NN
cord-004738-vnz15x84	98	20	(	(	-LRB-
cord-004738-vnz15x84	98	21	Fig	Fig	NNP
cord-004738-vnz15x84	98	22	.	.	NNP
cord-004738-vnz15x84	98	23	2	2	CD
cord-004738-vnz15x84	98	24	,	,	,
cord-004738-vnz15x84	98	25	lane	lane	NN
cord-004738-vnz15x84	98	26	10	10	CD
cord-004738-vnz15x84	98	27	,	,	,
cord-004738-vnz15x84	98	28	80-kDa	80-kda	CD
cord-004738-vnz15x84	98	29	protein	protein	NN
cord-004738-vnz15x84	98	30	)	)	-RRB-
cord-004738-vnz15x84	98	31	was	be	VBD
cord-004738-vnz15x84	98	32	cleaved	cleave	VBN
cord-004738-vnz15x84	98	33	by	by	IN
cord-004738-vnz15x84	98	34	the	the	DT
cord-004738-vnz15x84	98	35	protease	protease	NN
cord-004738-vnz15x84	98	36	at	at	IN
cord-004738-vnz15x84	98	37	the	the	DT
cord-004738-vnz15x84	98	38	cleavage	cleavage	NN
cord-004738-vnz15x84	98	39	site	site	NN
cord-004738-vnz15x84	98	40	,	,	,
cord-004738-vnz15x84	98	41	based	base	VBN
cord-004738-vnz15x84	98	42	on	on	IN
cord-004738-vnz15x84	98	43	the	the	DT
cord-004738-vnz15x84	98	44	expected	expect	VBN
cord-004738-vnz15x84	98	45	molecular	molecular	JJ
cord-004738-vnz15x84	98	46	weight	weight	NN
cord-004738-vnz15x84	98	47	of	of	IN
cord-004738-vnz15x84	98	48	the	the	DT
cord-004738-vnz15x84	98	49	cleaved	cleaved	JJ
cord-004738-vnz15x84	98	50	N	n	CD
cord-004738-vnz15x84	98	51	-	-	HYPH
cord-004738-vnz15x84	98	52	terminal	terminal	NN
cord-004738-vnz15x84	98	53	product	product	NN
cord-004738-vnz15x84	98	54	of	of	IN
cord-004738-vnz15x84	98	55	55	55	CD
cord-004738-vnz15x84	98	56	kDa	kDa	NNS
cord-004738-vnz15x84	98	57	(	(	-LRB-
cord-004738-vnz15x84	98	58	Fig	Fig	NNP
cord-004738-vnz15x84	98	59	.	.	NNP
cord-004738-vnz15x84	98	60	2	2	CD
cord-004738-vnz15x84	98	61	,	,	,
cord-004738-vnz15x84	98	62	lane	lane	NN
cord-004738-vnz15x84	98	63	11	11	CD
cord-004738-vnz15x84	98	64	)	)	-RRB-
cord-004738-vnz15x84	98	65	.	.	.
cord-004738-vnz15x84	99	1	This	this	DT
cord-004738-vnz15x84	99	2	clearly	clearly	RB
cord-004738-vnz15x84	99	3	suggests	suggest	VBZ
cord-004738-vnz15x84	99	4	that	that	IN
cord-004738-vnz15x84	99	5	the	the	DT
cord-004738-vnz15x84	99	6	protease	protease	NN
cord-004738-vnz15x84	99	7	was	be	VBD
cord-004738-vnz15x84	99	8	able	able	JJ
cord-004738-vnz15x84	99	9	to	to	TO
cord-004738-vnz15x84	99	10	cleave	cleave	VB
cord-004738-vnz15x84	99	11	the	the	DT
cord-004738-vnz15x84	99	12	RVS	RVS	NNP
cord-004738-vnz15x84	99	13	-	-	HYPH
cord-004738-vnz15x84	99	14	GFP	GFP	NNP
cord-004738-vnz15x84	99	15	substrate	substrate	NN
cord-004738-vnz15x84	99	16	with	with	IN
cord-004738-vnz15x84	99	17	a	a	DT
cord-004738-vnz15x84	99	18	GFP	GFP	NNP
cord-004738-vnz15x84	99	19	on	on	IN
cord-004738-vnz15x84	99	20	the	the	DT
cord-004738-vnz15x84	99	21	C	C	NNP
cord-004738-vnz15x84	99	22	-	-	HYPH
cord-004738-vnz15x84	99	23	terminal	terminal	NN
cord-004738-vnz15x84	99	24	side	side	NN
cord-004738-vnz15x84	99	25	of	of	IN
cord-004738-vnz15x84	99	26	the	the	DT
cord-004738-vnz15x84	99	27	cleavage	cleavage	NN
cord-004738-vnz15x84	99	28	site	site	NN
cord-004738-vnz15x84	99	29	,	,	,
cord-004738-vnz15x84	99	30	but	but	CC
cord-004738-vnz15x84	99	31	to	to	IN
cord-004738-vnz15x84	99	32	a	a	DT
cord-004738-vnz15x84	99	33	lesser	less	JJR
cord-004738-vnz15x84	99	34	extent	extent	NN
cord-004738-vnz15x84	99	35	(	(	-LRB-
cord-004738-vnz15x84	99	36	<	<	XX
cord-004738-vnz15x84	99	37	50	50	CD
cord-004738-vnz15x84	99	38	%	%	NN
cord-004738-vnz15x84	99	39	)	)	-RRB-
cord-004738-vnz15x84	99	40	,	,	,
cord-004738-vnz15x84	99	41	as	as	IN
cord-004738-vnz15x84	99	42	a	a	DT
cord-004738-vnz15x84	99	43	significant	significant	JJ
cord-004738-vnz15x84	99	44	portion	portion	NN
cord-004738-vnz15x84	99	45	of	of	IN
cord-004738-vnz15x84	99	46	the	the	DT
cord-004738-vnz15x84	99	47	substrate	substrate	NN
cord-004738-vnz15x84	99	48	was	be	VBD
cord-004738-vnz15x84	99	49	reproducibly	reproducibly	RB
cord-004738-vnz15x84	99	50	observed	observe	VBN
cord-004738-vnz15x84	99	51	to	to	TO
cord-004738-vnz15x84	99	52	be	be	VB
cord-004738-vnz15x84	99	53	 	 	_SP
cord-004738-vnz15x84	99	54	The	the	DT
cord-004738-vnz15x84	99	55	fact	fact	NN
cord-004738-vnz15x84	99	56	that	that	IN
cord-004738-vnz15x84	99	57	309-substrate	309-substrate	CD
cord-004738-vnz15x84	99	58	was	be	VBD
cord-004738-vnz15x84	99	59	cleaved	cleave	VBN
cord-004738-vnz15x84	99	60	and	and	CC
cord-004738-vnz15x84	99	61	199-substrate	199-substrate	CD
cord-004738-vnz15x84	99	62	was	be	VBD
cord-004738-vnz15x84	99	63	not	not	RB
cord-004738-vnz15x84	99	64	suggested	suggest	VBN
cord-004738-vnz15x84	99	65	that	that	IN
cord-004738-vnz15x84	99	66	trans	trans	JJ
cord-004738-vnz15x84	99	67	-	-	JJ
cord-004738-vnz15x84	99	68	protease	protease	JJ
cord-004738-vnz15x84	99	69	processing	processing	NN
cord-004738-vnz15x84	99	70	of	of	IN
cord-004738-vnz15x84	99	71	the	the	DT
cord-004738-vnz15x84	99	72	substrate	substrate	NN
cord-004738-vnz15x84	99	73	either	either	CC
cord-004738-vnz15x84	99	74	requires	require	VBZ
cord-004738-vnz15x84	99	75	a	a	DT
cord-004738-vnz15x84	99	76	minimum	minimum	JJ
cord-004738-vnz15x84	99	77	N	n	CD
cord-004738-vnz15x84	99	78	-	-	HYPH
cord-004738-vnz15x84	99	79	terminal	terminal	NN
cord-004738-vnz15x84	99	80	length	length	NN
cord-004738-vnz15x84	99	81	from	from	IN
cord-004738-vnz15x84	99	82	the	the	DT
cord-004738-vnz15x84	99	83	cleavage	cleavage	NN
cord-004738-vnz15x84	99	84	site	site	NN
cord-004738-vnz15x84	99	85	in	in	IN
cord-004738-vnz15x84	99	86	the	the	DT
cord-004738-vnz15x84	99	87	substrate	substrate	NN
cord-004738-vnz15x84	99	88	,	,	,
cord-004738-vnz15x84	99	89	or	or	CC
cord-004738-vnz15x84	99	90	it	-PRON-	PRP
cord-004738-vnz15x84	99	91	recognizes	recognize	VBZ
cord-004738-vnz15x84	99	92	an	an	DT
cord-004738-vnz15x84	99	93	internal	internal	JJ
cord-004738-vnz15x84	99	94	amino	amino	NN
cord-004738-vnz15x84	99	95	acid	acid	NN
cord-004738-vnz15x84	99	96	sequence	sequence	NN
cord-004738-vnz15x84	99	97	within	within	IN
cord-004738-vnz15x84	99	98	the	the	DT
cord-004738-vnz15x84	99	99	309-substrate	309-substrate	CD
cord-004738-vnz15x84	99	100	that	that	WDT
cord-004738-vnz15x84	99	101	is	be	VBZ
cord-004738-vnz15x84	99	102	lacking	lack	VBG
cord-004738-vnz15x84	99	103	in	in	IN
cord-004738-vnz15x84	99	104	the	the	DT
cord-004738-vnz15x84	99	105	199-substrate	199-substrate	CD
cord-004738-vnz15x84	99	106	.	.	.
cord-004738-vnz15x84	100	1	If	if	IN
cord-004738-vnz15x84	100	2	indeed	indeed	RB
cord-004738-vnz15x84	100	3	an	an	DT
cord-004738-vnz15x84	100	4	internal	internal	JJ
cord-004738-vnz15x84	100	5	domain	domain	NN
cord-004738-vnz15x84	100	6	within	within	IN
cord-004738-vnz15x84	100	7	the	the	DT
cord-004738-vnz15x84	100	8	309-substrate	309-substrate	CD
cord-004738-vnz15x84	100	9	is	be	VBZ
cord-004738-vnz15x84	100	10	being	be	VBG
cord-004738-vnz15x84	100	11	recognized	recognize	VBN
cord-004738-vnz15x84	100	12	by	by	IN
cord-004738-vnz15x84	100	13	the	the	DT
cord-004738-vnz15x84	100	14	protease	protease	NN
cord-004738-vnz15x84	100	15	,	,	,
cord-004738-vnz15x84	100	16	then	then	RB
cord-004738-vnz15x84	100	17	this	this	DT
cord-004738-vnz15x84	100	18	sequence	sequence	NN
cord-004738-vnz15x84	100	19	should	should	MD
cord-004738-vnz15x84	100	20	be	be	VB
cord-004738-vnz15x84	100	21	present	present	JJ
cord-004738-vnz15x84	100	22	in	in	IN
cord-004738-vnz15x84	100	23	the	the	DT
cord-004738-vnz15x84	100	24	N	n	CD
cord-004738-vnz15x84	100	25	-	-	HYPH
cord-004738-vnz15x84	100	26	terminal	terminal	NN
cord-004738-vnz15x84	100	27	region	region	NN
cord-004738-vnz15x84	100	28	of	of	IN
cord-004738-vnz15x84	100	29	309-substrate	309-substrate	CD
cord-004738-vnz15x84	100	30	.	.	.
cord-004738-vnz15x84	101	1	To	to	TO
cord-004738-vnz15x84	101	2	verify	verify	VB
cord-004738-vnz15x84	101	3	the	the	DT
cord-004738-vnz15x84	101	4	above	above	JJ
cord-004738-vnz15x84	101	5	hypothesis	hypothesis	NN
cord-004738-vnz15x84	101	6	,	,	,
cord-004738-vnz15x84	101	7	we	-PRON-	PRP
cord-004738-vnz15x84	101	8	utilized	utilize	VBD
cord-004738-vnz15x84	101	9	plasmid	plasmid	NN
cord-004738-vnz15x84	101	10	pRVS	prvs	JJ
cord-004738-vnz15x84	101	11	-	-	HYPH
cord-004738-vnz15x84	101	12	GFP-1102	gfp-1102	NN
cord-004738-vnz15x84	101	13	-	-	HYPH
cord-004738-vnz15x84	101	14	1548	1548	CD
cord-004738-vnz15x84	102	1	(	(	-LRB-
cord-004738-vnz15x84	102	2	Fig	Fig	NNP
cord-004738-vnz15x84	102	3	.	.	NNP
cord-004738-vnz15x84	102	4	1	1	CD
cord-004738-vnz15x84	102	5	)	)	-RRB-
cord-004738-vnz15x84	102	6	,	,	,
cord-004738-vnz15x84	102	7	which	which	WDT
cord-004738-vnz15x84	102	8	expresses	express	VBZ
cord-004738-vnz15x84	102	9	a	a	DT
cord-004738-vnz15x84	102	10	chimeric	chimeric	JJ
cord-004738-vnz15x84	102	11	substrate	substrate	NN
cord-004738-vnz15x84	102	12	in	in	IN
cord-004738-vnz15x84	102	13	which	which	WDT
cord-004738-vnz15x84	102	14	the	the	DT
cord-004738-vnz15x84	102	15	199-substrate	199-substrate	CD
cord-004738-vnz15x84	102	16	was	be	VBD
cord-004738-vnz15x84	102	17	extended	extend	VBN
cord-004738-vnz15x84	102	18	at	at	IN
cord-004738-vnz15x84	102	19	its	-PRON-	PRP$
cord-004738-vnz15x84	102	20	N	n	CD
cord-004738-vnz15x84	102	21	-	-	HYPH
cord-004738-vnz15x84	102	22	terminus	terminus	NN
cord-004738-vnz15x84	102	23	by	by	IN
cord-004738-vnz15x84	102	24	GFP	GFP	NNP
cord-004738-vnz15x84	102	25	to	to	TO
cord-004738-vnz15x84	102	26	compensate	compensate	VB
cord-004738-vnz15x84	102	27	for	for	IN
cord-004738-vnz15x84	102	28	the	the	DT
cord-004738-vnz15x84	102	29	length	length	NN
cord-004738-vnz15x84	102	30	(	(	-LRB-
cord-004738-vnz15x84	102	31	increased	increase	VBD
cord-004738-vnz15x84	102	32	from	from	IN
cord-004738-vnz15x84	102	33	199	199	CD
cord-004738-vnz15x84	102	34	to	to	TO
cord-004738-vnz15x84	102	35	479	479	CD
cord-004738-vnz15x84	102	36	amino	amino	JJ
cord-004738-vnz15x84	102	37	acids	acid	NNS
cord-004738-vnz15x84	102	38	)	)	-RRB-
cord-004738-vnz15x84	102	39	along	along	IN
cord-004738-vnz15x84	102	40	with	with	IN
cord-004738-vnz15x84	102	41	the	the	DT
cord-004738-vnz15x84	102	42	protease	protease	NN
cord-004738-vnz15x84	102	43	plasmid	plasmid	NN
cord-004738-vnz15x84	102	44	,	,	,
cord-004738-vnz15x84	102	45	pRVP	prvp	JJ
cord-004738-vnz15x84	102	46	.	.	.
cord-004738-vnz15x84	103	1	Immunoblot	Immunoblot	NNP
cord-004738-vnz15x84	103	2	analysis	analysis	NN
cord-004738-vnz15x84	104	1	(	(	-LRB-
cord-004738-vnz15x84	104	2	Fig	Fig	NNP
cord-004738-vnz15x84	104	3	.	.	.
cord-004738-vnz15x84	104	4	3	3	LS
cord-004738-vnz15x84	104	5	)	)	-RRB-
cord-004738-vnz15x84	104	6	demonstrated	demonstrate	VBD
cord-004738-vnz15x84	104	7	that	that	IN
cord-004738-vnz15x84	104	8	when	when	WRB
cord-004738-vnz15x84	104	9	the	the	DT
cord-004738-vnz15x84	104	10	protease	protease	NN
cord-004738-vnz15x84	104	11	(	(	-LRB-
cord-004738-vnz15x84	104	12	Lane	lane	NN
cord-004738-vnz15x84	104	13	1	1	CD
cord-004738-vnz15x84	104	14	)	)	-RRB-
cord-004738-vnz15x84	104	15	and	and	CC
cord-004738-vnz15x84	104	16	a	a	DT
cord-004738-vnz15x84	104	17	positive	positive	JJ
cord-004738-vnz15x84	104	18	control	control	NN
cord-004738-vnz15x84	104	19	475substrate	475substrate	NN
cord-004738-vnz15x84	105	1	(	(	-LRB-
cord-004738-vnz15x84	105	2	Lane	lane	NN
cord-004738-vnz15x84	105	3	2	2	CD
cord-004738-vnz15x84	105	4	)	)	-RRB-
cord-004738-vnz15x84	105	5	were	be	VBD
cord-004738-vnz15x84	105	6	coexpressed	coexpresse	VBN
cord-004738-vnz15x84	105	7	,	,	,
cord-004738-vnz15x84	105	8	the	the	DT
cord-004738-vnz15x84	105	9	substrate	substrate	NN
cord-004738-vnz15x84	105	10	did	do	VBD
cord-004738-vnz15x84	105	11	undergo	undergo	VB
cord-004738-vnz15x84	105	12	cleavage	cleavage	NN
cord-004738-vnz15x84	105	13	(	(	-LRB-
cord-004738-vnz15x84	105	14	Lane	lane	NN
cord-004738-vnz15x84	105	15	3	3	CD
cord-004738-vnz15x84	105	16	)	)	-RRB-
cord-004738-vnz15x84	105	17	.	.	.
cord-004738-vnz15x84	106	1	Although	although	IN
cord-004738-vnz15x84	106	2	the	the	DT
cord-004738-vnz15x84	106	3	N	n	CD
cord-004738-vnz15x84	106	4	-	-	HYPH
cord-004738-vnz15x84	106	5	terminal	terminal	JJ
cord-004738-vnz15x84	106	6	length	length	NN
cord-004738-vnz15x84	106	7	was	be	VBD
cord-004738-vnz15x84	106	8	increased	increase	VBN
cord-004738-vnz15x84	106	9	in	in	IN
cord-004738-vnz15x84	106	10	the	the	DT
cord-004738-vnz15x84	106	11	chimeric	chimeric	JJ
cord-004738-vnz15x84	106	12	GFP-199-substrate	GFP-199-substrate	NNP
cord-004738-vnz15x84	106	13	(	(	-LRB-
cord-004738-vnz15x84	106	14	Lane	Lane	NNP
cord-004738-vnz15x84	106	15	4	4	CD
cord-004738-vnz15x84	106	16	)	)	-RRB-
cord-004738-vnz15x84	106	17	,	,	,
cord-004738-vnz15x84	106	18	it	-PRON-	PRP
cord-004738-vnz15x84	106	19	still	still	RB
cord-004738-vnz15x84	106	20	failed	fail	VBD
cord-004738-vnz15x84	106	21	to	to	TO
cord-004738-vnz15x84	106	22	undergo	undergo	VB
cord-004738-vnz15x84	106	23	cleavage	cleavage	NN
cord-004738-vnz15x84	106	24	when	when	WRB
cord-004738-vnz15x84	106	25	coexpressed	coexpresse	VBN
cord-004738-vnz15x84	106	26	with	with	IN
cord-004738-vnz15x84	106	27	the	the	DT
cord-004738-vnz15x84	106	28	protease	protease	NN
cord-004738-vnz15x84	106	29	(	(	-LRB-
cord-004738-vnz15x84	106	30	Lane	lane	NN
cord-004738-vnz15x84	106	31	5	5	CD
cord-004738-vnz15x84	106	32	)	)	-RRB-
cord-004738-vnz15x84	106	33	,	,	,
cord-004738-vnz15x84	106	34	suggesting	suggest	VBG
cord-004738-vnz15x84	106	35	that	that	IN
cord-004738-vnz15x84	106	36	it	-PRON-	PRP
cord-004738-vnz15x84	106	37	is	be	VBZ
cord-004738-vnz15x84	106	38	not	not	RB
cord-004738-vnz15x84	106	39	the	the	DT
cord-004738-vnz15x84	106	40	N	n	CD
cord-004738-vnz15x84	106	41	-	-	HYPH
cord-004738-vnz15x84	106	42	terminal	terminal	NN
cord-004738-vnz15x84	106	43	length	length	NN
cord-004738-vnz15x84	106	44	from	from	IN
cord-004738-vnz15x84	106	45	the	the	DT
cord-004738-vnz15x84	106	46	cleavage	cleavage	NN
cord-004738-vnz15x84	106	47	site	site	NN
cord-004738-vnz15x84	106	48	that	that	WDT
cord-004738-vnz15x84	106	49	is	be	VBZ
cord-004738-vnz15x84	106	50	required	require	VBN
cord-004738-vnz15x84	106	51	of	of	IN
cord-004738-vnz15x84	106	52	the	the	DT
cord-004738-vnz15x84	106	53	substrate	substrate	NN
cord-004738-vnz15x84	106	54	,	,	,
cord-004738-vnz15x84	106	55	but	but	CC
cord-004738-vnz15x84	106	56	in	in	IN
cord-004738-vnz15x84	106	57	fact	fact	NN
cord-004738-vnz15x84	106	58	it	-PRON-	PRP
cord-004738-vnz15x84	106	59	is	be	VBZ
cord-004738-vnz15x84	106	60	the	the	DT
cord-004738-vnz15x84	106	61	internal	internal	JJ
cord-004738-vnz15x84	106	62	sequence	sequence	NN
cord-004738-vnz15x84	106	63	present	present	JJ
cord-004738-vnz15x84	106	64	within	within	IN
cord-004738-vnz15x84	106	65	the	the	DT
cord-004738-vnz15x84	106	66	substrate	substrate	NN
cord-004738-vnz15x84	106	67	that	that	WDT
cord-004738-vnz15x84	106	68	is	be	VBZ
cord-004738-vnz15x84	106	69	recognized	recognize	VBN
cord-004738-vnz15x84	106	70	by	by	IN
cord-004738-vnz15x84	106	71	the	the	DT
cord-004738-vnz15x84	106	72	protease	protease	NN
cord-004738-vnz15x84	106	73	for	for	IN
cord-004738-vnz15x84	106	74	trans	trans	NN
cord-004738-vnz15x84	106	75	-	-	NN
cord-004738-vnz15x84	106	76	processing	processing	NN
cord-004738-vnz15x84	106	77	.	.	.
cord-004738-vnz15x84	107	1	The	the	DT
cord-004738-vnz15x84	107	2	internal	internal	JJ
cord-004738-vnz15x84	107	3	recognition	recognition	NN
cord-004738-vnz15x84	107	4	domain	domain	NN
cord-004738-vnz15x84	107	5	identified	identify	VBN
cord-004738-vnz15x84	107	6	in	in	IN
cord-004738-vnz15x84	107	7	this	this	DT
cord-004738-vnz15x84	107	8	report	report	NN
cord-004738-vnz15x84	107	9	corresponds	correspond	VBZ
cord-004738-vnz15x84	107	10	to	to	IN
cord-004738-vnz15x84	107	11	amino	amino	NN
cord-004738-vnz15x84	107	12	acid	acid	NN
cord-004738-vnz15x84	107	13	[	[	-LRB-
cord-004738-vnz15x84	107	14	8	8	CD
cord-004738-vnz15x84	107	15	]	]	-RRB-
cord-004738-vnz15x84	107	16	.	.	.
cord-004738-vnz15x84	108	1	In	in	IN
cord-004738-vnz15x84	108	2	the	the	DT
cord-004738-vnz15x84	108	3	bottom	bottom	JJ
cord-004738-vnz15x84	108	4	panel	panel	NN
cord-004738-vnz15x84	108	5	,	,	,
cord-004738-vnz15x84	108	6	the	the	DT
cord-004738-vnz15x84	108	7	protease	protease	NN
cord-004738-vnz15x84	108	8	substrate	substrate	NN
cord-004738-vnz15x84	108	9	P200	P200	NNP
cord-004738-vnz15x84	108	10	is	be	VBZ
cord-004738-vnz15x84	108	11	depicted	depict	VBN
cord-004738-vnz15x84	108	12	with	with	IN
cord-004738-vnz15x84	108	13	the	the	DT
cord-004738-vnz15x84	108	14	location	location	NN
cord-004738-vnz15x84	108	15	of	of	IN
cord-004738-vnz15x84	108	16	the	the	DT
cord-004738-vnz15x84	108	17	newly	newly	RB
cord-004738-vnz15x84	108	18	identified	identify	VBN
cord-004738-vnz15x84	108	19	domain	domain	NN
cord-004738-vnz15x84	108	20	required	require	VBN
cord-004738-vnz15x84	108	21	for	for	IN
cord-004738-vnz15x84	108	22	protease	protease	JJ
cord-004738-vnz15x84	108	23	trans	trans	NN
cord-004738-vnz15x84	108	24	-	-	NN
cord-004738-vnz15x84	108	25	activity	activity	NN
cord-004738-vnz15x84	108	26	(	(	-LRB-
cord-004738-vnz15x84	108	27	this	this	DT
cord-004738-vnz15x84	108	28	report	report	NN
cord-004738-vnz15x84	108	29	)	)	-RRB-
cord-004738-vnz15x84	108	30	,	,	,
cord-004738-vnz15x84	108	31	which	which	WDT
cord-004738-vnz15x84	108	32	overlaps	overlap	VBZ
cord-004738-vnz15x84	108	33	with	with	IN
cord-004738-vnz15x84	108	34	the	the	DT
cord-004738-vnz15x84	108	35	protease	protease	NN
cord-004738-vnz15x84	108	36	cis	cis	NN
cord-004738-vnz15x84	108	37	-	-	HYPH
cord-004738-vnz15x84	108	38	activity	activity	NN
cord-004738-vnz15x84	108	39	starting	starting	NN
cord-004738-vnz15x84	108	40	site	site	NN
cord-004738-vnz15x84	108	41	of	of	IN
cord-004738-vnz15x84	108	42	P200	p200	NN
cord-004738-vnz15x84	108	43	position	position	NN
cord-004738-vnz15x84	108	44	994	994	CD
cord-004738-vnz15x84	108	45	-	-	HYPH
cord-004738-vnz15x84	108	46	1102	1102	CD
cord-004738-vnz15x84	108	47	of	of	IN
cord-004738-vnz15x84	108	48	RV	RV	NNP
cord-004738-vnz15x84	108	49	P200	P200	NNP
cord-004738-vnz15x84	108	50	.	.	.
cord-004738-vnz15x84	109	1	This	this	DT
cord-004738-vnz15x84	109	2	region	region	NN
cord-004738-vnz15x84	109	3	overlaps	overlap	VBZ
cord-004738-vnz15x84	109	4	with	with	IN
cord-004738-vnz15x84	109	5	the	the	DT
cord-004738-vnz15x84	109	6	N	n	CD
cord-004738-vnz15x84	109	7	-	-	HYPH
cord-004738-vnz15x84	109	8	terminal	terminal	NN
cord-004738-vnz15x84	109	9	site	site	NN
cord-004738-vnz15x84	109	10	of	of	IN
cord-004738-vnz15x84	109	11	the	the	DT
cord-004738-vnz15x84	109	12	essential	essential	JJ
cord-004738-vnz15x84	109	13	cis	cis	JJ
cord-004738-vnz15x84	109	14	-	-	HYPH
cord-004738-vnz15x84	109	15	protease	protease	JJ
cord-004738-vnz15x84	109	16	activity	activity	NN
cord-004738-vnz15x84	109	17	region	region	NN
cord-004738-vnz15x84	109	18	on	on	IN
cord-004738-vnz15x84	109	19	the	the	DT
cord-004738-vnz15x84	109	20	P200	p200	NN
cord-004738-vnz15x84	109	21	described	describe	VBN
cord-004738-vnz15x84	109	22	by	by	IN
cord-004738-vnz15x84	109	23	Liang	Liang	NNP
cord-004738-vnz15x84	109	24	et	et	NNP
cord-004738-vnz15x84	109	25	al	al	NNP
cord-004738-vnz15x84	109	26	.	.	.
cord-004738-vnz15x84	110	1	[	[	-LRB-
cord-004738-vnz15x84	110	2	8	8	CD
cord-004738-vnz15x84	110	3	]	]	-RRB-
cord-004738-vnz15x84	110	4	.	.	.
cord-004738-vnz15x84	111	1	The	the	DT
cord-004738-vnz15x84	111	2	location	location	NN
cord-004738-vnz15x84	111	3	of	of	IN
cord-004738-vnz15x84	111	4	the	the	DT
cord-004738-vnz15x84	111	5	substrate	substrate	NN
cord-004738-vnz15x84	111	6	recognition	recognition	NN
cord-004738-vnz15x84	111	7	region	region	NN
cord-004738-vnz15x84	111	8	(	(	-LRB-
cord-004738-vnz15x84	111	9	this	this	DT
cord-004738-vnz15x84	111	10	report	report	NN
cord-004738-vnz15x84	111	11	)	)	-RRB-
cord-004738-vnz15x84	111	12	relative	relative	JJ
cord-004738-vnz15x84	111	13	to	to	IN
cord-004738-vnz15x84	111	14	the	the	DT
cord-004738-vnz15x84	111	15	essential	essential	JJ
cord-004738-vnz15x84	111	16	cis	cis	JJ
cord-004738-vnz15x84	111	17	-	-	HYPH
cord-004738-vnz15x84	111	18	activity	activity	NN
cord-004738-vnz15x84	111	19	domain	domain	NN
cord-004738-vnz15x84	111	20	is	be	VBZ
cord-004738-vnz15x84	111	21	illustrated	illustrate	VBN
cord-004738-vnz15x84	111	22	in	in	IN
cord-004738-vnz15x84	111	23	Fig	Fig	NNP
cord-004738-vnz15x84	111	24	.	.	.
cord-004738-vnz15x84	112	1	4	4	LS
cord-004738-vnz15x84	112	2	.	.	.
cord-004738-vnz15x84	113	1	At	at	IN
cord-004738-vnz15x84	113	2	this	this	DT
cord-004738-vnz15x84	113	3	time	time	NN
cord-004738-vnz15x84	113	4	we	-PRON-	PRP
cord-004738-vnz15x84	113	5	could	could	MD
cord-004738-vnz15x84	113	6	not	not	RB
cord-004738-vnz15x84	113	7	refine	refine	VB
cord-004738-vnz15x84	113	8	the	the	DT
cord-004738-vnz15x84	113	9	recognition	recognition	NN
cord-004738-vnz15x84	113	10	region	region	NN
cord-004738-vnz15x84	113	11	further	further	RB
cord-004738-vnz15x84	113	12	,	,	,
cord-004738-vnz15x84	113	13	as	as	IN
cord-004738-vnz15x84	113	14	plasmids	plasmid	NNS
cord-004738-vnz15x84	113	15	with	with	IN
cord-004738-vnz15x84	113	16	deletions	deletion	NNS
cord-004738-vnz15x84	113	17	in	in	IN
cord-004738-vnz15x84	113	18	this	this	DT
cord-004738-vnz15x84	113	19	region	region	NN
cord-004738-vnz15x84	113	20	of	of	IN
cord-004738-vnz15x84	113	21	RV	RV	NNP
cord-004738-vnz15x84	113	22	cDNA	cdna	NN
cord-004738-vnz15x84	113	23	could	could	MD
cord-004738-vnz15x84	113	24	not	not	RB
cord-004738-vnz15x84	113	25	be	be	VB
cord-004738-vnz15x84	113	26	rescued	rescue	VBN
cord-004738-vnz15x84	113	27	in	in	IN
cord-004738-vnz15x84	113	28	bacteria	bacteria	NNS
cord-004738-vnz15x84	113	29	.	.	.
cord-004738-vnz15x84	114	1	In	in	IN
cord-004738-vnz15x84	114	2	this	this	DT
cord-004738-vnz15x84	114	3	report	report	NN
cord-004738-vnz15x84	114	4	,	,	,
cord-004738-vnz15x84	114	5	we	-PRON-	PRP
cord-004738-vnz15x84	114	6	defined	define	VBD
cord-004738-vnz15x84	114	7	the	the	DT
cord-004738-vnz15x84	114	8	minimal	minimal	JJ
cord-004738-vnz15x84	114	9	substrate	substrate	NN
cord-004738-vnz15x84	114	10	(	(	-LRB-
cord-004738-vnz15x84	114	11	template	template	NN
cord-004738-vnz15x84	114	12	)	)	-RRB-
cord-004738-vnz15x84	114	13	for	for	IN
cord-004738-vnz15x84	114	14	proteolytic	proteolytic	JJ
cord-004738-vnz15x84	114	15	processing	processing	NN
cord-004738-vnz15x84	114	16	in	in	IN
cord-004738-vnz15x84	114	17	trans	trans	NNP
cord-004738-vnz15x84	114	18	by	by	IN
cord-004738-vnz15x84	114	19	the	the	DT
cord-004738-vnz15x84	114	20	rubella	rubella	NN
cord-004738-vnz15x84	114	21	virus	virus	NN
cord-004738-vnz15x84	114	22	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	114	23	protease	protease	NN
cord-004738-vnz15x84	114	24	using	use	VBG
cord-004738-vnz15x84	114	25	an	an	DT
cord-004738-vnz15x84	114	26	epitopetagged	epitopetagge	VBN
cord-004738-vnz15x84	114	27	protein	protein	NN
cord-004738-vnz15x84	114	28	expression	expression	NN
cord-004738-vnz15x84	114	29	system	system	NN
cord-004738-vnz15x84	114	30	.	.	.
cord-004738-vnz15x84	115	1	Results	result	NNS
cord-004738-vnz15x84	115	2	presented	present	VBN
cord-004738-vnz15x84	115	3	with	with	IN
cord-004738-vnz15x84	115	4	RV	rv	NN
cord-004738-vnz15x84	115	5	protease	protease	NN
cord-004738-vnz15x84	115	6	(	(	-LRB-
cord-004738-vnz15x84	115	7	which	which	WDT
cord-004738-vnz15x84	115	8	has	have	VBZ
cord-004738-vnz15x84	115	9	been	be	VBN
cord-004738-vnz15x84	115	10	functionally	functionally	RB
cord-004738-vnz15x84	115	11	demonstrated	demonstrate	VBN
cord-004738-vnz15x84	115	12	to	to	TO
cord-004738-vnz15x84	115	13	be	be	VB
cord-004738-vnz15x84	115	14	similar	similar	JJ
cord-004738-vnz15x84	115	15	to	to	IN
cord-004738-vnz15x84	115	16	P150	p150	CD
cord-004738-vnz15x84	115	17	activity	activity	NN
cord-004738-vnz15x84	115	18	[	[	-LRB-
cord-004738-vnz15x84	115	19	8	8	CD
cord-004738-vnz15x84	115	20	]	]	-RRB-
cord-004738-vnz15x84	115	21	)	)	-RRB-
cord-004738-vnz15x84	115	22	demonstrated	demonstrate	VBD
cord-004738-vnz15x84	115	23	that	that	IN
cord-004738-vnz15x84	115	24	the	the	DT
cord-004738-vnz15x84	115	25	protease	protease	NN
cord-004738-vnz15x84	115	26	trans	trans	NN
cord-004738-vnz15x84	115	27	-	-	NN
cord-004738-vnz15x84	115	28	activity	activity	NN
cord-004738-vnz15x84	115	29	recognizes	recognize	VBZ
cord-004738-vnz15x84	115	30	a	a	DT
cord-004738-vnz15x84	115	31	region	region	NN
cord-004738-vnz15x84	115	32	N	n	CD
cord-004738-vnz15x84	115	33	-	-	HYPH
cord-004738-vnz15x84	115	34	terminal	terminal	NN
cord-004738-vnz15x84	115	35	to	to	IN
cord-004738-vnz15x84	115	36	the	the	DT
cord-004738-vnz15x84	115	37	cleavage	cleavage	NN
cord-004738-vnz15x84	115	38	site	site	NN
cord-004738-vnz15x84	115	39	in	in	IN
cord-004738-vnz15x84	115	40	the	the	DT
cord-004738-vnz15x84	115	41	P200-derived	p200-derived	JJ
cord-004738-vnz15x84	115	42	substrates	substrate	NNS
cord-004738-vnz15x84	115	43	.	.	.
cord-004738-vnz15x84	116	1	Thus	thus	RB
cord-004738-vnz15x84	116	2	,	,	,
cord-004738-vnz15x84	116	3	the	the	DT
cord-004738-vnz15x84	116	4	trans	trans	JJ
cord-004738-vnz15x84	116	5	-	-	JJ
cord-004738-vnz15x84	116	6	protease	protease	JJ
cord-004738-vnz15x84	116	7	activity	activity	NN
cord-004738-vnz15x84	116	8	requires	require	VBZ
cord-004738-vnz15x84	116	9	not	not	RB
cord-004738-vnz15x84	116	10	only	only	RB
cord-004738-vnz15x84	116	11	specific	specific	JJ
cord-004738-vnz15x84	116	12	amino	amino	NN
cord-004738-vnz15x84	116	13	acids	acid	NNS
cord-004738-vnz15x84	116	14	present	present	JJ
cord-004738-vnz15x84	116	15	at	at	IN
cord-004738-vnz15x84	116	16	or	or	CC
cord-004738-vnz15x84	116	17	proximal	proximal	JJ
cord-004738-vnz15x84	116	18	to	to	IN
cord-004738-vnz15x84	116	19	the	the	DT
cord-004738-vnz15x84	116	20	cleavage	cleavage	NN
cord-004738-vnz15x84	116	21	site	site	NN
cord-004738-vnz15x84	116	22	,	,	,
cord-004738-vnz15x84	116	23	but	but	CC
cord-004738-vnz15x84	116	24	also	also	RB
cord-004738-vnz15x84	116	25	sequences	sequence	NNS
cord-004738-vnz15x84	116	26	upstream	upstream	RB
cord-004738-vnz15x84	116	27	at	at	IN
cord-004738-vnz15x84	116	28	a	a	DT
cord-004738-vnz15x84	116	29	distance	distance	NN
cord-004738-vnz15x84	116	30	from	from	IN
cord-004738-vnz15x84	116	31	the	the	DT
cord-004738-vnz15x84	116	32	cleavage	cleavage	NN
cord-004738-vnz15x84	116	33	site	site	NN
cord-004738-vnz15x84	116	34	.	.	.
cord-004738-vnz15x84	117	1	We	-PRON-	PRP
cord-004738-vnz15x84	117	2	also	also	RB
cord-004738-vnz15x84	117	3	demonstrated	demonstrate	VBD
cord-004738-vnz15x84	117	4	that	that	IN
cord-004738-vnz15x84	117	5	,	,	,
cord-004738-vnz15x84	117	6	on	on	IN
cord-004738-vnz15x84	117	7	the	the	DT
cord-004738-vnz15x84	117	8	C	C	NNP
cord-004738-vnz15x84	117	9	-	-	HYPH
cord-004738-vnz15x84	117	10	terminal	terminal	NN
cord-004738-vnz15x84	117	11	side	side	NN
cord-004738-vnz15x84	117	12	of	of	IN
cord-004738-vnz15x84	117	13	the	the	DT
cord-004738-vnz15x84	117	14	cleavage	cleavage	NN
cord-004738-vnz15x84	117	15	site	site	NN
cord-004738-vnz15x84	117	16	,	,	,
cord-004738-vnz15x84	117	17	heterologous	heterologous	JJ
cord-004738-vnz15x84	117	18	residues	residue	NNS
cord-004738-vnz15x84	117	19	unrelated	unrelated	JJ
cord-004738-vnz15x84	117	20	to	to	IN
cord-004738-vnz15x84	117	21	RV	RV	NNP
cord-004738-vnz15x84	117	22	are	be	VBP
cord-004738-vnz15x84	117	23	somewhat	somewhat	RB
cord-004738-vnz15x84	117	24	tolerated	tolerate	VBN
cord-004738-vnz15x84	117	25	by	by	IN
cord-004738-vnz15x84	117	26	the	the	DT
cord-004738-vnz15x84	117	27	protease	protease	NN
cord-004738-vnz15x84	117	28	,	,	,
cord-004738-vnz15x84	117	29	but	but	CC
cord-004738-vnz15x84	117	30	we	-PRON-	PRP
cord-004738-vnz15x84	117	31	routinely	routinely	RB
cord-004738-vnz15x84	117	32	observed	observe	VBD
cord-004738-vnz15x84	117	33	(	(	-LRB-
cord-004738-vnz15x84	117	34	in	in	IN
cord-004738-vnz15x84	117	35	three	three	CD
cord-004738-vnz15x84	117	36	separate	separate	JJ
cord-004738-vnz15x84	117	37	experiments	experiment	NNS
cord-004738-vnz15x84	117	38	)	)	-RRB-
cord-004738-vnz15x84	117	39	that	that	IN
cord-004738-vnz15x84	117	40	the	the	DT
cord-004738-vnz15x84	117	41	efficiency	efficiency	NN
cord-004738-vnz15x84	117	42	of	of	IN
cord-004738-vnz15x84	117	43	the	the	DT
cord-004738-vnz15x84	117	44	protease	protease	NN
cord-004738-vnz15x84	117	45	trans	trans	NN
cord-004738-vnz15x84	117	46	-	-	NN
cord-004738-vnz15x84	117	47	activity	activity	NN
cord-004738-vnz15x84	117	48	on	on	IN
cord-004738-vnz15x84	117	49	this	this	DT
cord-004738-vnz15x84	117	50	type	type	NN
cord-004738-vnz15x84	117	51	of	of	IN
cord-004738-vnz15x84	117	52	substrate	substrate	NN
cord-004738-vnz15x84	117	53	was	be	VBD
cord-004738-vnz15x84	117	54	less	less	JJR
cord-004738-vnz15x84	117	55	than	than	IN
cord-004738-vnz15x84	117	56	50	50	CD
cord-004738-vnz15x84	117	57	%	%	NN
cord-004738-vnz15x84	117	58	(	(	-LRB-
cord-004738-vnz15x84	117	59	illustrated	illustrate	VBN
cord-004738-vnz15x84	117	60	in	in	IN
cord-004738-vnz15x84	117	61	Fig	Fig	NNP
cord-004738-vnz15x84	117	62	.	.	.
cord-004738-vnz15x84	118	1	2	2	LS
cord-004738-vnz15x84	118	2	,	,	,
cord-004738-vnz15x84	118	3	lane	lane	NN
cord-004738-vnz15x84	118	4	11	11	CD
cord-004738-vnz15x84	118	5	)	)	-RRB-
cord-004738-vnz15x84	118	6	.	.	.
cord-004738-vnz15x84	119	1	This	this	DT
cord-004738-vnz15x84	119	2	clearly	clearly	RB
cord-004738-vnz15x84	119	3	suggests	suggest	VBZ
cord-004738-vnz15x84	119	4	that	that	IN
cord-004738-vnz15x84	119	5	the	the	DT
cord-004738-vnz15x84	119	6	P90	P90	NNP
cord-004738-vnz15x84	119	7	domain	domain	NN
cord-004738-vnz15x84	119	8	residing	reside	VBG
cord-004738-vnz15x84	119	9	on	on	IN
cord-004738-vnz15x84	119	10	the	the	DT
cord-004738-vnz15x84	119	11	C	C	NNP
cord-004738-vnz15x84	119	12	-	-	HYPH
cord-004738-vnz15x84	119	13	terminal	terminal	NN
cord-004738-vnz15x84	119	14	side	side	NN
cord-004738-vnz15x84	119	15	of	of	IN
cord-004738-vnz15x84	119	16	the	the	DT
cord-004738-vnz15x84	119	17	cleavage	cleavage	NN
cord-004738-vnz15x84	119	18	site	site	NN
cord-004738-vnz15x84	119	19	in	in	IN
cord-004738-vnz15x84	119	20	P200	P200	NNP
cord-004738-vnz15x84	119	21	does	do	VBZ
cord-004738-vnz15x84	119	22	influence	influence	VB
cord-004738-vnz15x84	119	23	the	the	DT
cord-004738-vnz15x84	119	24	trans	trans	NN
cord-004738-vnz15x84	119	25	-	-	NN
cord-004738-vnz15x84	119	26	activity	activity	NN
cord-004738-vnz15x84	119	27	of	of	IN
cord-004738-vnz15x84	119	28	the	the	DT
cord-004738-vnz15x84	119	29	protease	protease	NN
cord-004738-vnz15x84	119	30	.	.	.
cord-004738-vnz15x84	120	1	In	in	IN
cord-004738-vnz15x84	120	2	another	another	DT
cord-004738-vnz15x84	120	3	positivestranded	positivestranded	JJ
cord-004738-vnz15x84	120	4	RNA	RNA	NNP
cord-004738-vnz15x84	120	5	virus	virus	NN
cord-004738-vnz15x84	120	6	,	,	,
cord-004738-vnz15x84	120	7	mouse	mouse	NNP
cord-004738-vnz15x84	120	8	hepatitis	hepatitis	NN
cord-004738-vnz15x84	120	9	virus	virus	NN
cord-004738-vnz15x84	120	10	(	(	-LRB-
cord-004738-vnz15x84	120	11	MHV	MHV	NNP
cord-004738-vnz15x84	120	12	)	)	-RRB-
cord-004738-vnz15x84	120	13	,	,	,
cord-004738-vnz15x84	120	14	one	one	CD
cord-004738-vnz15x84	120	15	of	of	IN
cord-004738-vnz15x84	120	16	the	the	DT
cord-004738-vnz15x84	120	17	two	two	CD
cord-004738-vnz15x84	120	18	virus	virus	NN
cord-004738-vnz15x84	120	19	-	-	HYPH
cord-004738-vnz15x84	120	20	encoded	encode	VBN
cord-004738-vnz15x84	120	21	proteases	protease	NNS
cord-004738-vnz15x84	120	22	,	,	,
cord-004738-vnz15x84	120	23	PLP-1	PLP-1	NNP
cord-004738-vnz15x84	120	24	(	(	-LRB-
cord-004738-vnz15x84	120	25	PCP	PCP	NNP
cord-004738-vnz15x84	120	26	)	)	-RRB-
cord-004738-vnz15x84	120	27	,	,	,
cord-004738-vnz15x84	120	28	demonstrates	demonstrate	VBZ
cord-004738-vnz15x84	120	29	a	a	DT
cord-004738-vnz15x84	120	30	homologous	homologous	JJ
cord-004738-vnz15x84	120	31	substrate	substrate	NN
cord-004738-vnz15x84	120	32	length	length	NN
cord-004738-vnz15x84	120	33	requirement	requirement	NN
cord-004738-vnz15x84	120	34	on	on	IN
cord-004738-vnz15x84	120	35	the	the	DT
cord-004738-vnz15x84	120	36	C	C	NNP
cord-004738-vnz15x84	120	37	-	-	HYPH
cord-004738-vnz15x84	120	38	terminal	terminal	NN
cord-004738-vnz15x84	120	39	side	side	NN
cord-004738-vnz15x84	120	40	of	of	IN
cord-004738-vnz15x84	120	41	the	the	DT
cord-004738-vnz15x84	120	42	cleavage	cleavage	NN
cord-004738-vnz15x84	120	43	site	site	NN
cord-004738-vnz15x84	120	44	and	and	CC
cord-004738-vnz15x84	120	45	the	the	DT
cord-004738-vnz15x84	120	46	cleavage	cleavage	NN
cord-004738-vnz15x84	120	47	efficiency	efficiency	NN
cord-004738-vnz15x84	120	48	increases	increase	VBZ
cord-004738-vnz15x84	120	49	with	with	IN
cord-004738-vnz15x84	120	50	increasing	increase	VBG
cord-004738-vnz15x84	120	51	substrate	substrate	NN
cord-004738-vnz15x84	120	52	and	and	CC
cord-004738-vnz15x84	120	53	enzyme	enzyme	NN
cord-004738-vnz15x84	120	54	polypeptide	polypeptide	NN
cord-004738-vnz15x84	120	55	length	length	NN
cord-004738-vnz15x84	120	56	,	,	,
cord-004738-vnz15x84	120	57	although	although	IN
cord-004738-vnz15x84	120	58	in	in	IN
cord-004738-vnz15x84	120	59	this	this	DT
cord-004738-vnz15x84	120	60	case	case	NN
cord-004738-vnz15x84	120	61	,	,	,
cord-004738-vnz15x84	120	62	the	the	DT
cord-004738-vnz15x84	120	63	protease	protease	NN
cord-004738-vnz15x84	120	64	recognition	recognition	NN
cord-004738-vnz15x84	120	65	sequence	sequence	NN
cord-004738-vnz15x84	120	66	on	on	IN
cord-004738-vnz15x84	120	67	the	the	DT
cord-004738-vnz15x84	120	68	substrate	substrate	NN
cord-004738-vnz15x84	120	69	was	be	VBD
cord-004738-vnz15x84	120	70	not	not	RB
cord-004738-vnz15x84	120	71	identified	identify	VBN
cord-004738-vnz15x84	120	72	[	[	-LRB-
cord-004738-vnz15x84	120	73	15	15	CD
cord-004738-vnz15x84	120	74	]	]	-RRB-
cord-004738-vnz15x84	120	75	.	.	.
cord-004738-vnz15x84	121	1	The	the	DT
cord-004738-vnz15x84	121	2	RV	RV	NNP
cord-004738-vnz15x84	121	3	protease	protease	NN
cord-004738-vnz15x84	121	4	trans	trans	JJ
cord-004738-vnz15x84	121	5	-	-	NN
cord-004738-vnz15x84	121	6	activity	activity	NN
cord-004738-vnz15x84	121	7	-	-	HYPH
cord-004738-vnz15x84	121	8	associated	associate	VBN
cord-004738-vnz15x84	121	9	P200	P200	NNP
cord-004738-vnz15x84	121	10	internal	internal	JJ
cord-004738-vnz15x84	121	11	sequence	sequence	NN
cord-004738-vnz15x84	121	12	requirement	requirement	NN
cord-004738-vnz15x84	121	13	,	,	,
cord-004738-vnz15x84	121	14	as	as	IN
cord-004738-vnz15x84	121	15	identified	identify	VBN
cord-004738-vnz15x84	121	16	here	here	RB
cord-004738-vnz15x84	121	17	,	,	,
cord-004738-vnz15x84	121	18	is	be	VBZ
cord-004738-vnz15x84	121	19	unique	unique	JJ
cord-004738-vnz15x84	121	20	,	,	,
cord-004738-vnz15x84	121	21	and	and	CC
cord-004738-vnz15x84	121	22	this	this	DT
cord-004738-vnz15x84	121	23	is	be	VBZ
cord-004738-vnz15x84	121	24	the	the	DT
cord-004738-vnz15x84	121	25	first	first	JJ
cord-004738-vnz15x84	121	26	such	such	JJ
cord-004738-vnz15x84	121	27	report	report	NN
cord-004738-vnz15x84	121	28	for	for	IN
cord-004738-vnz15x84	121	29	the	the	DT
cord-004738-vnz15x84	121	30	viruses	virus	NNS
cord-004738-vnz15x84	121	31	that	that	WDT
cord-004738-vnz15x84	121	32	belong	belong	VBP
cord-004738-vnz15x84	121	33	to	to	IN
cord-004738-vnz15x84	121	34	the	the	DT
cord-004738-vnz15x84	121	35	family	family	NN
cord-004738-vnz15x84	121	36	Togaviridae	Togaviridae	NNP
cord-004738-vnz15x84	121	37	.	.	.
cord-004738-vnz15x84	122	1	In	in	IN
cord-004738-vnz15x84	122	2	this	this	DT
cord-004738-vnz15x84	122	3	study	study	NN
cord-004738-vnz15x84	122	4	,	,	,
cord-004738-vnz15x84	122	5	we	-PRON-	PRP
cord-004738-vnz15x84	122	6	mapped	map	VBD
cord-004738-vnz15x84	122	7	the	the	DT
cord-004738-vnz15x84	122	8	regions	region	NNS
cord-004738-vnz15x84	122	9	of	of	IN
cord-004738-vnz15x84	122	10	the	the	DT
cord-004738-vnz15x84	122	11	protease	protease	NN
cord-004738-vnz15x84	122	12	substrate	substrate	NN
cord-004738-vnz15x84	122	13	required	require	VBN
cord-004738-vnz15x84	122	14	for	for	IN
cord-004738-vnz15x84	122	15	trans	trans	NN
cord-004738-vnz15x84	122	16	-	-	NN
cord-004738-vnz15x84	122	17	activity	activity	NN
cord-004738-vnz15x84	122	18	,	,	,
cord-004738-vnz15x84	122	19	which	which	WDT
cord-004738-vnz15x84	122	20	is	be	VBZ
cord-004738-vnz15x84	122	21	reciprocal	reciprocal	JJ
cord-004738-vnz15x84	122	22	to	to	IN
cord-004738-vnz15x84	122	23	the	the	DT
cord-004738-vnz15x84	122	24	Liang	Liang	NNP
cord-004738-vnz15x84	122	25	et	et	NNP
cord-004738-vnz15x84	122	26	al	al	NNP
cord-004738-vnz15x84	122	27	.	.	.
cord-004738-vnz15x84	123	1	study	study	NN
cord-004738-vnz15x84	123	2	wherein	wherein	WRB
cord-004738-vnz15x84	123	3	they	-PRON-	PRP
cord-004738-vnz15x84	123	4	mapped	map	VBD
cord-004738-vnz15x84	123	5	the	the	DT
cord-004738-vnz15x84	123	6	essential	essential	JJ
cord-004738-vnz15x84	123	7	cis	cis	NNP
cord-004738-vnz15x84	123	8	-	-	HYPH
cord-004738-vnz15x84	123	9	and	and	CC
cord-004738-vnz15x84	123	10	trans	trans	JJ
cord-004738-vnz15x84	123	11	-	-	NN
cord-004738-vnz15x84	123	12	activity	activity	JJ
cord-004738-vnz15x84	123	13	regions	region	NNS
cord-004738-vnz15x84	123	14	of	of	IN
cord-004738-vnz15x84	123	15	the	the	DT
cord-004738-vnz15x84	123	16	protease	protease	NN
cord-004738-vnz15x84	123	17	itself	-PRON-	PRP
cord-004738-vnz15x84	123	18	and	and	CC
cord-004738-vnz15x84	123	19	also	also	RB
cord-004738-vnz15x84	123	20	demonstrated	demonstrate	VBD
cord-004738-vnz15x84	123	21	that	that	IN
cord-004738-vnz15x84	123	22	the	the	DT
cord-004738-vnz15x84	123	23	X	x	NN
cord-004738-vnz15x84	123	24	domain	domain	NN
cord-004738-vnz15x84	123	25	(	(	-LRB-
cord-004738-vnz15x84	123	26	proline	proline	NN
cord-004738-vnz15x84	123	27	-	-	HYPH
cord-004738-vnz15x84	123	28	rich	rich	JJ
cord-004738-vnz15x84	123	29	,	,	,
cord-004738-vnz15x84	123	30	conserved	conserve	VBN
cord-004738-vnz15x84	123	31	in	in	IN
cord-004738-vnz15x84	123	32	all	all	DT
cord-004738-vnz15x84	123	33	M	M	NNP
cord-004738-vnz15x84	123	34	-	-	HYPH
cord-004738-vnz15x84	123	35	group	group	NN
cord-004738-vnz15x84	123	36	PCPs	pcp	NNS
cord-004738-vnz15x84	123	37	)	)	-RRB-
cord-004738-vnz15x84	123	38	present	present	JJ
cord-004738-vnz15x84	123	39	in	in	IN
cord-004738-vnz15x84	123	40	the	the	DT
cord-004738-vnz15x84	123	41	RV	RV	NNP
cord-004738-vnz15x84	123	42	protease	protease	NN
cord-004738-vnz15x84	123	43	is	be	VBZ
cord-004738-vnz15x84	123	44	important	important	JJ
cord-004738-vnz15x84	123	45	for	for	IN
cord-004738-vnz15x84	123	46	the	the	DT
cord-004738-vnz15x84	123	47	protease	protease	NN
cord-004738-vnz15x84	123	48	trans	trans	NN
cord-004738-vnz15x84	123	49	-	-	NN
cord-004738-vnz15x84	123	50	activity	activity	NN
cord-004738-vnz15x84	123	51	[	[	-LRB-
cord-004738-vnz15x84	123	52	8	8	CD
cord-004738-vnz15x84	123	53	]	]	-RRB-
cord-004738-vnz15x84	123	54	.	.	.
cord-004738-vnz15x84	124	1	They	-PRON-	PRP
cord-004738-vnz15x84	124	2	further	further	RB
cord-004738-vnz15x84	124	3	speculated	speculate	VBD
cord-004738-vnz15x84	124	4	that	that	IN
cord-004738-vnz15x84	124	5	this	this	DT
cord-004738-vnz15x84	124	6	proline	proline	JJ
cord-004738-vnz15x84	124	7	-	-	HYPH
cord-004738-vnz15x84	124	8	rich	rich	JJ
cord-004738-vnz15x84	124	9	X	x	NN
cord-004738-vnz15x84	124	10	domain	domain	NN
cord-004738-vnz15x84	124	11	could	could	MD
cord-004738-vnz15x84	124	12	serve	serve	VB
cord-004738-vnz15x84	124	13	as	as	IN
cord-004738-vnz15x84	124	14	a	a	DT
cord-004738-vnz15x84	124	15	protein	protein	NN
cord-004738-vnz15x84	124	16	-	-	HYPH
cord-004738-vnz15x84	124	17	protein	protein	NN
cord-004738-vnz15x84	124	18	interaction	interaction	NN
cord-004738-vnz15x84	124	19	domain	domain	NN
cord-004738-vnz15x84	124	20	that	that	WDT
cord-004738-vnz15x84	124	21	enhances	enhance	VBZ
cord-004738-vnz15x84	124	22	the	the	DT
cord-004738-vnz15x84	124	23	opportunity	opportunity	NN
cord-004738-vnz15x84	124	24	to	to	TO
cord-004738-vnz15x84	124	25	meet	meet	VB
cord-004738-vnz15x84	124	26	its	-PRON-	PRP$
cord-004738-vnz15x84	124	27	trans	trans	JJ
cord-004738-vnz15x84	124	28	-	-	NN
cord-004738-vnz15x84	124	29	cleavage	cleavage	JJ
cord-004738-vnz15x84	124	30	substrate	substrate	NN
cord-004738-vnz15x84	124	31	[	[	-LRB-
cord-004738-vnz15x84	124	32	8	8	CD
cord-004738-vnz15x84	124	33	]	]	-RRB-
cord-004738-vnz15x84	124	34	.	.	.
cord-004738-vnz15x84	125	1	However	however	RB
cord-004738-vnz15x84	125	2	,	,	,
cord-004738-vnz15x84	125	3	in	in	IN
cord-004738-vnz15x84	125	4	this	this	DT
cord-004738-vnz15x84	125	5	report	report	NN
cord-004738-vnz15x84	125	6	,	,	,
cord-004738-vnz15x84	125	7	we	-PRON-	PRP
cord-004738-vnz15x84	125	8	clearly	clearly	RB
cord-004738-vnz15x84	125	9	demonstrate	demonstrate	VBP
cord-004738-vnz15x84	125	10	by	by	IN
cord-004738-vnz15x84	125	11	deletion	deletion	NN
cord-004738-vnz15x84	125	12	analysis	analysis	NN
cord-004738-vnz15x84	125	13	that	that	WDT
cord-004738-vnz15x84	125	14	the	the	DT
cord-004738-vnz15x84	125	15	X	x	NN
cord-004738-vnz15x84	125	16	domain	domain	NN
cord-004738-vnz15x84	125	17	in	in	IN
cord-004738-vnz15x84	125	18	the	the	DT
cord-004738-vnz15x84	125	19	substrate	substrate	NN
cord-004738-vnz15x84	125	20	is	be	VBZ
cord-004738-vnz15x84	125	21	dispensable	dispensable	JJ
cord-004738-vnz15x84	125	22	for	for	IN
cord-004738-vnz15x84	125	23	recognition	recognition	NN
cord-004738-vnz15x84	125	24	by	by	IN
cord-004738-vnz15x84	125	25	RV	RV	NNP
cord-004738-vnz15x84	125	26	protease	protease	NN
cord-004738-vnz15x84	125	27	trans	trans	NN
cord-004738-vnz15x84	125	28	-	-	NN
cord-004738-vnz15x84	125	29	activity	activity	NN
cord-004738-vnz15x84	125	30	and	and	CC
cord-004738-vnz15x84	125	31	the	the	DT
cord-004738-vnz15x84	125	32	actual	actual	JJ
cord-004738-vnz15x84	125	33	recognition	recognition	NN
cord-004738-vnz15x84	125	34	region	region	NN
cord-004738-vnz15x84	125	35	is	be	VBZ
cord-004738-vnz15x84	125	36	located	locate	VBN
cord-004738-vnz15x84	125	37	downstream	downstream	RB
cord-004738-vnz15x84	125	38	of	of	IN
cord-004738-vnz15x84	125	39	the	the	DT
cord-004738-vnz15x84	125	40	X	x	NN
cord-004738-vnz15x84	125	41	domain	domain	NN
cord-004738-vnz15x84	125	42	,	,	,
cord-004738-vnz15x84	125	43	overlapping	overlap	VBG
cord-004738-vnz15x84	125	44	with	with	IN
cord-004738-vnz15x84	125	45	the	the	DT
cord-004738-vnz15x84	125	46	N	n	CD
cord-004738-vnz15x84	125	47	-	-	HYPH
cord-004738-vnz15x84	125	48	terminal	terminal	NN
cord-004738-vnz15x84	125	49	starting	starting	NN
cord-004738-vnz15x84	125	50	-	-	HYPH
cord-004738-vnz15x84	125	51	site	site	NN
cord-004738-vnz15x84	125	52	(	(	-LRB-
cord-004738-vnz15x84	125	53	a	a	DT
cord-004738-vnz15x84	125	54	term	term	NN
cord-004738-vnz15x84	125	55	coined	coin	VBN
cord-004738-vnz15x84	125	56	by	by	IN
cord-004738-vnz15x84	125	57	Liang	Liang	NNP
cord-004738-vnz15x84	125	58	et	et	NNP
cord-004738-vnz15x84	125	59	al	al	NNP
cord-004738-vnz15x84	125	60	.	.	.
cord-004738-vnz15x84	126	1	[	[	-LRB-
cord-004738-vnz15x84	126	2	8	8	CD
cord-004738-vnz15x84	126	3	]	]	-RRB-
cord-004738-vnz15x84	126	4	)	)	-RRB-
cord-004738-vnz15x84	126	5	of	of	IN
cord-004738-vnz15x84	126	6	the	the	DT
cord-004738-vnz15x84	126	7	essential	essential	JJ
cord-004738-vnz15x84	126	8	cis	cis	JJ
cord-004738-vnz15x84	126	9	-	-	HYPH
cord-004738-vnz15x84	126	10	activity	activity	NN
cord-004738-vnz15x84	126	11	domain	domain	NN
cord-004738-vnz15x84	126	12	of	of	IN
cord-004738-vnz15x84	126	13	the	the	DT
cord-004738-vnz15x84	126	14	substrate	substrate	NN
cord-004738-vnz15x84	126	15	.	.	.
cord-004738-vnz15x84	127	1	Our	-PRON-	PRP$
cord-004738-vnz15x84	127	2	results	result	NNS
cord-004738-vnz15x84	127	3	show	show	VBP
cord-004738-vnz15x84	127	4	that	that	IN
cord-004738-vnz15x84	127	5	RV	RV	NNP
cord-004738-vnz15x84	127	6	P150-associated	p150-associate	VBN
cord-004738-vnz15x84	127	7	protease	protease	NN
cord-004738-vnz15x84	127	8	trans	trans	NN
cord-004738-vnz15x84	127	9	-	-	NN
cord-004738-vnz15x84	127	10	activity	activity	NN
cord-004738-vnz15x84	127	11	requires	require	VBZ
cord-004738-vnz15x84	127	12	a	a	DT
cord-004738-vnz15x84	127	13	specific	specific	JJ
cord-004738-vnz15x84	127	14	region	region	NN
cord-004738-vnz15x84	127	15	within	within	IN
cord-004738-vnz15x84	127	16	the	the	DT
cord-004738-vnz15x84	127	17	P200	p200	NN
cord-004738-vnz15x84	127	18	that	that	WDT
cord-004738-vnz15x84	127	19	represents	represent	VBZ
cord-004738-vnz15x84	127	20	P150	p150	CD
cord-004738-vnz15x84	127	21	itself	-PRON-	PRP
cord-004738-vnz15x84	128	1	(	(	-LRB-
cord-004738-vnz15x84	128	2	illustrated	illustrate	VBN
cord-004738-vnz15x84	128	3	in	in	IN
cord-004738-vnz15x84	128	4	Fig	Fig	NNP
cord-004738-vnz15x84	128	5	.	.	NNP
cord-004738-vnz15x84	128	6	4	4	CD
cord-004738-vnz15x84	128	7	)	)	-RRB-
cord-004738-vnz15x84	128	8	.	.	.
cord-004738-vnz15x84	129	1	Taken	take	VBN
cord-004738-vnz15x84	129	2	together	together	RB
cord-004738-vnz15x84	129	3	,	,	,
cord-004738-vnz15x84	129	4	our	-PRON-	PRP$
cord-004738-vnz15x84	129	5	studies	study	NNS
cord-004738-vnz15x84	129	6	and	and	CC
cord-004738-vnz15x84	129	7	those	those	DT
cord-004738-vnz15x84	129	8	of	of	IN
cord-004738-vnz15x84	129	9	Liang	Liang	NNP
cord-004738-vnz15x84	129	10	et	et	NNP
cord-004738-vnz15x84	129	11	al	al	NNP
cord-004738-vnz15x84	129	12	.	.	.
cord-004738-vnz15x84	130	1	[	[	-LRB-
cord-004738-vnz15x84	130	2	8	8	CD
cord-004738-vnz15x84	130	3	]	]	-RRB-
cord-004738-vnz15x84	130	4	advance	advance	VB
cord-004738-vnz15x84	130	5	the	the	DT
cord-004738-vnz15x84	130	6	field	field	NN
cord-004738-vnz15x84	130	7	in	in	IN
cord-004738-vnz15x84	130	8	enhancing	enhance	VBG
cord-004738-vnz15x84	130	9	our	-PRON-	PRP$
cord-004738-vnz15x84	130	10	understanding	understanding	NN
cord-004738-vnz15x84	130	11	of	of	IN
cord-004738-vnz15x84	130	12	the	the	DT
cord-004738-vnz15x84	130	13	molecular	molecular	JJ
cord-004738-vnz15x84	130	14	determinants	determinant	NNS
cord-004738-vnz15x84	130	15	that	that	WDT
cord-004738-vnz15x84	130	16	define	define	VBP
cord-004738-vnz15x84	130	17	the	the	DT
cord-004738-vnz15x84	130	18	rubivirus	rubivirus	NN
cord-004738-vnz15x84	130	19	protease	protease	NN
cord-004738-vnz15x84	130	20	trans	trans	NN
cord-004738-vnz15x84	130	21	-	-	NN
cord-004738-vnz15x84	130	22	activity	activity	NN
cord-004738-vnz15x84	130	23	requirements	requirement	NNS
cord-004738-vnz15x84	130	24	that	that	WDT
cord-004738-vnz15x84	130	25	are	be	VBP
cord-004738-vnz15x84	130	26	essential	essential	JJ
cord-004738-vnz15x84	130	27	for	for	IN
cord-004738-vnz15x84	130	28	RV	rv	NN
cord-004738-vnz15x84	130	29	replication	replication	NN
cord-004738-vnz15x84	130	30	.	.	.
cord-004738-vnz15x84	131	1	In	in	IN
cord-004738-vnz15x84	131	2	this	this	DT
cord-004738-vnz15x84	131	3	report	report	NN
cord-004738-vnz15x84	131	4	,	,	,
cord-004738-vnz15x84	131	5	we	-PRON-	PRP
cord-004738-vnz15x84	131	6	have	have	VBP
cord-004738-vnz15x84	131	7	shown	show	VBN
cord-004738-vnz15x84	131	8	that	that	IN
cord-004738-vnz15x84	131	9	RV	RV	NNP
cord-004738-vnz15x84	131	10	protease	protease	NN
cord-004738-vnz15x84	131	11	trans	trans	NN
cord-004738-vnz15x84	131	12	-	-	NN
cord-004738-vnz15x84	131	13	activity	activity	NN
cord-004738-vnz15x84	131	14	demonstrates	demonstrate	VBZ
cord-004738-vnz15x84	131	15	substrate	substrate	JJ
cord-004738-vnz15x84	131	16	specificity	specificity	NN
cord-004738-vnz15x84	131	17	by	by	IN
cord-004738-vnz15x84	131	18	requiring	require	VBG
cord-004738-vnz15x84	131	19	an	an	DT
cord-004738-vnz15x84	131	20	internal	internal	JJ
cord-004738-vnz15x84	131	21	sequence	sequence	NN
cord-004738-vnz15x84	131	22	within	within	IN
cord-004738-vnz15x84	131	23	the	the	DT
cord-004738-vnz15x84	131	24	region	region	NN
cord-004738-vnz15x84	131	25	that	that	WDT
cord-004738-vnz15x84	131	26	is	be	VBZ
cord-004738-vnz15x84	131	27	N	n	CD
cord-004738-vnz15x84	131	28	-	-	NN
cord-004738-vnz15x84	131	29	terminal	terminal	NN
cord-004738-vnz15x84	131	30	to	to	IN
cord-004738-vnz15x84	131	31	the	the	DT
cord-004738-vnz15x84	131	32	cleavage	cleavage	NN
cord-004738-vnz15x84	131	33	site	site	NN
cord-004738-vnz15x84	131	34	.	.	.
cord-004738-vnz15x84	132	1	Identification	identification	NN
cord-004738-vnz15x84	132	2	of	of	IN
cord-004738-vnz15x84	132	3	a	a	DT
cord-004738-vnz15x84	132	4	region	region	NN
cord-004738-vnz15x84	132	5	in	in	IN
cord-004738-vnz15x84	132	6	the	the	DT
cord-004738-vnz15x84	132	7	P200-related	p200-related	JJ
cord-004738-vnz15x84	132	8	sequence	sequence	NN
cord-004738-vnz15x84	132	9	-	-	HYPH
cord-004738-vnz15x84	132	10	containing	contain	VBG
cord-004738-vnz15x84	132	11	substrate	substrate	NN
cord-004738-vnz15x84	132	12	(	(	-LRB-
cord-004738-vnz15x84	132	13	we	-PRON-	PRP
cord-004738-vnz15x84	132	14	utilized	utilize	VBN
cord-004738-vnz15x84	132	15	polypeptides	polypeptide	NNS
cord-004738-vnz15x84	132	16	from	from	IN
cord-004738-vnz15x84	132	17	amino	amino	NN
cord-004738-vnz15x84	132	18	acid	acid	NN
cord-004738-vnz15x84	132	19	positions	position	NNS
cord-004738-vnz15x84	132	20	827	827	CD
cord-004738-vnz15x84	132	21	to1548	to1548	NN
cord-004738-vnz15x84	132	22	of	of	IN
cord-004738-vnz15x84	132	23	P200	p200	NN
cord-004738-vnz15x84	132	24	or	or	CC
cord-004738-vnz15x84	132	25	shorter	short	JJR
cord-004738-vnz15x84	132	26	)	)	-RRB-
cord-004738-vnz15x84	132	27	that	that	WDT
cord-004738-vnz15x84	132	28	is	be	VBZ
cord-004738-vnz15x84	132	29	important	important	JJ
cord-004738-vnz15x84	132	30	for	for	IN
cord-004738-vnz15x84	132	31	RV	rv	NN
cord-004738-vnz15x84	132	32	protease	protease	NN
cord-004738-vnz15x84	132	33	transactivity	transactivity	NN
cord-004738-vnz15x84	132	34	suggests	suggest	VBZ
cord-004738-vnz15x84	132	35	that	that	IN
cord-004738-vnz15x84	132	36	this	this	DT
cord-004738-vnz15x84	132	37	region	region	NN
cord-004738-vnz15x84	132	38	may	may	MD
cord-004738-vnz15x84	132	39	offer	offer	VB
cord-004738-vnz15x84	132	40	a	a	DT
cord-004738-vnz15x84	132	41	specific	specific	JJ
cord-004738-vnz15x84	132	42	fold	fold	NN
cord-004738-vnz15x84	132	43	or	or	CC
cord-004738-vnz15x84	132	44	a	a	DT
cord-004738-vnz15x84	132	45	conformation	conformation	NN
cord-004738-vnz15x84	132	46	to	to	IN
cord-004738-vnz15x84	132	47	the	the	DT
cord-004738-vnz15x84	132	48	P200	p200	NN
cord-004738-vnz15x84	132	49	substrate	substrate	NN
cord-004738-vnz15x84	132	50	to	to	TO
cord-004738-vnz15x84	132	51	facilitate	facilitate	VB
cord-004738-vnz15x84	132	52	cleavage	cleavage	NN
cord-004738-vnz15x84	132	53	by	by	IN
cord-004738-vnz15x84	132	54	the	the	DT
cord-004738-vnz15x84	132	55	protease	protease	NN
cord-004738-vnz15x84	132	56	.	.	.
cord-004738-vnz15x84	133	1	Since	since	IN
cord-004738-vnz15x84	133	2	most	most	JJS
cord-004738-vnz15x84	133	3	protease	protease	NN
cord-004738-vnz15x84	133	4	-	-	HYPH
cord-004738-vnz15x84	133	5	substrate	substrate	NN
cord-004738-vnz15x84	133	6	interactions	interaction	NNS
cord-004738-vnz15x84	133	7	involve	involve	VBP
cord-004738-vnz15x84	133	8	transient	transient	JJ
cord-004738-vnz15x84	133	9	binding	binding	NN
cord-004738-vnz15x84	133	10	of	of	IN
cord-004738-vnz15x84	133	11	the	the	DT
cord-004738-vnz15x84	133	12	protease	protease	NN
cord-004738-vnz15x84	133	13	to	to	IN
cord-004738-vnz15x84	133	14	its	-PRON-	PRP$
cord-004738-vnz15x84	133	15	cognate	cognate	JJ
cord-004738-vnz15x84	133	16	substrate	substrate	NN
cord-004738-vnz15x84	133	17	,	,	,
cord-004738-vnz15x84	133	18	it	-PRON-	PRP
cord-004738-vnz15x84	133	19	is	be	VBZ
cord-004738-vnz15x84	133	20	conceivable	conceivable	JJ
cord-004738-vnz15x84	133	21	that	that	IN
cord-004738-vnz15x84	133	22	RV	rv	NN
cord-004738-vnz15x84	133	23	protease	protease	NN
cord-004738-vnz15x84	133	24	trans	trans	NN
cord-004738-vnz15x84	133	25	-	-	NN
cord-004738-vnz15x84	133	26	activity	activity	NN
cord-004738-vnz15x84	133	27	on	on	IN
cord-004738-vnz15x84	133	28	homologous	homologous	JJ
cord-004738-vnz15x84	133	29	substrates	substrate	NNS
cord-004738-vnz15x84	133	30	also	also	RB
cord-004738-vnz15x84	133	31	involves	involve	VBZ
cord-004738-vnz15x84	133	32	transient	transient	JJ
cord-004738-vnz15x84	133	33	binding	binding	NN
cord-004738-vnz15x84	133	34	of	of	IN
cord-004738-vnz15x84	133	35	protease	protease	NN
cord-004738-vnz15x84	133	36	to	to	IN
cord-004738-vnz15x84	133	37	the	the	DT
cord-004738-vnz15x84	133	38	substrate	substrate	NN
cord-004738-vnz15x84	133	39	,	,	,
cord-004738-vnz15x84	133	40	and	and	CC
cord-004738-vnz15x84	133	41	such	such	JJ
cord-004738-vnz15x84	133	42	binding	binding	NN
cord-004738-vnz15x84	133	43	perhaps	perhaps	RB
cord-004738-vnz15x84	133	44	could	could	MD
cord-004738-vnz15x84	133	45	occur	occur	VB
cord-004738-vnz15x84	133	46	within	within	IN
cord-004738-vnz15x84	133	47	the	the	DT
cord-004738-vnz15x84	133	48	sequence	sequence	NN
cord-004738-vnz15x84	133	49	that	that	WDT
cord-004738-vnz15x84	133	50	is	be	VBZ
cord-004738-vnz15x84	133	51	required	require	VBN
cord-004738-vnz15x84	133	52	on	on	IN
cord-004738-vnz15x84	133	53	the	the	DT
cord-004738-vnz15x84	133	54	substrate	substrate	NN
cord-004738-vnz15x84	133	55	for	for	IN
cord-004738-vnz15x84	133	56	protease	protease	NN
cord-004738-vnz15x84	133	57	transactivity	transactivity	NN
cord-004738-vnz15x84	133	58	.	.	.
cord-004738-vnz15x84	134	1	We	-PRON-	PRP
cord-004738-vnz15x84	134	2	attempted	attempt	VBD
cord-004738-vnz15x84	134	3	to	to	TO
cord-004738-vnz15x84	134	4	perform	perform	VB
cord-004738-vnz15x84	134	5	coimmunoprecipitation	coimmunoprecipitation	NN
cord-004738-vnz15x84	134	6	experiments	experiment	NNS
cord-004738-vnz15x84	134	7	to	to	TO
cord-004738-vnz15x84	134	8	establish	establish	VB
cord-004738-vnz15x84	134	9	the	the	DT
cord-004738-vnz15x84	134	10	protease	protease	NN
cord-004738-vnz15x84	134	11	-	-	HYPH
cord-004738-vnz15x84	134	12	substrate	substrate	NN
cord-004738-vnz15x84	134	13	binding	binding	NN
cord-004738-vnz15x84	134	14	following	follow	VBG
cord-004738-vnz15x84	134	15	cotransfection	cotransfection	NN
cord-004738-vnz15x84	134	16	of	of	IN
cord-004738-vnz15x84	134	17	cells	cell	NNS
cord-004738-vnz15x84	134	18	with	with	IN
cord-004738-vnz15x84	134	19	both	both	DT
cord-004738-vnz15x84	134	20	plasmid	plasmid	NN
cord-004738-vnz15x84	134	21	constructs	construct	NNS
cord-004738-vnz15x84	134	22	,	,	,
cord-004738-vnz15x84	134	23	but	but	CC
cord-004738-vnz15x84	134	24	failed	fail	VBD
cord-004738-vnz15x84	134	25	to	to	TO
cord-004738-vnz15x84	134	26	obtain	obtain	VB
cord-004738-vnz15x84	134	27	reproducible	reproducible	JJ
cord-004738-vnz15x84	134	28	results	result	NNS
cord-004738-vnz15x84	134	29	,	,	,
cord-004738-vnz15x84	134	30	perhaps	perhaps	RB
cord-004738-vnz15x84	134	31	due	due	IN
cord-004738-vnz15x84	134	32	to	to	IN
cord-004738-vnz15x84	134	33	the	the	DT
cord-004738-vnz15x84	134	34	transient	transient	JJ
cord-004738-vnz15x84	134	35	nature	nature	NN
cord-004738-vnz15x84	134	36	of	of	IN
cord-004738-vnz15x84	134	37	the	the	DT
cord-004738-vnz15x84	134	38	interaction	interaction	NN
cord-004738-vnz15x84	134	39	.	.	.
cord-004738-vnz15x84	135	1	However	however	RB
cord-004738-vnz15x84	135	2	,	,	,
cord-004738-vnz15x84	135	3	if	if	IN
cord-004738-vnz15x84	135	4	this	this	DT
cord-004738-vnz15x84	135	5	binding	binding	NN
cord-004738-vnz15x84	135	6	truly	truly	RB
cord-004738-vnz15x84	135	7	occurs	occur	VBZ
cord-004738-vnz15x84	135	8	in	in	IN
cord-004738-vnz15x84	135	9	the	the	DT
cord-004738-vnz15x84	135	10	viral	viral	JJ
cord-004738-vnz15x84	135	11	infection	infection	NN
cord-004738-vnz15x84	135	12	cycle	cycle	NN
cord-004738-vnz15x84	135	13	,	,	,
cord-004738-vnz15x84	135	14	then	then	RB
cord-004738-vnz15x84	135	15	,	,	,
cord-004738-vnz15x84	135	16	as	as	IN
cord-004738-vnz15x84	135	17	RV	rv	NN
cord-004738-vnz15x84	135	18	protease	protease	NN
cord-004738-vnz15x84	135	19	is	be	VBZ
cord-004738-vnz15x84	135	20	recognizing	recognize	VBG
cord-004738-vnz15x84	135	21	itself	-PRON-	PRP
cord-004738-vnz15x84	135	22	as	as	IN
cord-004738-vnz15x84	135	23	substrate	substrate	NN
cord-004738-vnz15x84	135	24	in	in	IN
cord-004738-vnz15x84	135	25	the	the	DT
cord-004738-vnz15x84	135	26	essential	essential	JJ
cord-004738-vnz15x84	135	27	cis	cis	JJ
cord-004738-vnz15x84	135	28	-	-	HYPH
cord-004738-vnz15x84	135	29	activity	activity	NN
cord-004738-vnz15x84	135	30	region	region	NN
cord-004738-vnz15x84	135	31	for	for	IN
cord-004738-vnz15x84	135	32	the	the	DT
cord-004738-vnz15x84	135	33	trans	trans	JJ
cord-004738-vnz15x84	135	34	-	-	NN
cord-004738-vnz15x84	135	35	activity	activity	NN
cord-004738-vnz15x84	135	36	(	(	-LRB-
cord-004738-vnz15x84	135	37	illustrated	illustrate	VBN
cord-004738-vnz15x84	135	38	in	in	IN
cord-004738-vnz15x84	135	39	Fig	Fig	NNP
cord-004738-vnz15x84	135	40	.	.	NNP
cord-004738-vnz15x84	135	41	4	4	CD
cord-004738-vnz15x84	135	42	)	)	-RRB-
cord-004738-vnz15x84	135	43	,	,	,
cord-004738-vnz15x84	135	44	it	-PRON-	PRP
cord-004738-vnz15x84	135	45	is	be	VBZ
cord-004738-vnz15x84	135	46	tempting	tempt	VBG
cord-004738-vnz15x84	135	47	to	to	TO
cord-004738-vnz15x84	135	48	speculate	speculate	VB
cord-004738-vnz15x84	135	49	that	that	IN
cord-004738-vnz15x84	135	50	the	the	DT
cord-004738-vnz15x84	135	51	trans	trans	NN
cord-004738-vnz15x84	135	52	-	-	NN
cord-004738-vnz15x84	135	53	activity	activity	NN
cord-004738-vnz15x84	135	54	may	may	MD
cord-004738-vnz15x84	135	55	be	be	VB
cord-004738-vnz15x84	135	56	regulating	regulate	VBG
cord-004738-vnz15x84	135	57	the	the	DT
cord-004738-vnz15x84	135	58	cis	cis	NNP
cord-004738-vnz15x84	135	59	-	-	HYPH
cord-004738-vnz15x84	135	60	activity	activity	NN
cord-004738-vnz15x84	135	61	of	of	IN
cord-004738-vnz15x84	135	62	P200	p200	NN
cord-004738-vnz15x84	135	63	.	.	.
cord-004738-vnz15x84	136	1	This	this	DT
cord-004738-vnz15x84	136	2	process	process	NN
cord-004738-vnz15x84	136	3	could	could	MD
cord-004738-vnz15x84	136	4	explain	explain	VB
cord-004738-vnz15x84	136	5	how	how	WRB
cord-004738-vnz15x84	136	6	P200	P200	NNP
cord-004738-vnz15x84	136	7	remains	remain	VBZ
cord-004738-vnz15x84	136	8	as	as	IN
cord-004738-vnz15x84	136	9	P200	p200	NN
cord-004738-vnz15x84	136	10	in	in	IN
cord-004738-vnz15x84	136	11	the	the	DT
cord-004738-vnz15x84	136	12	replication	replication	NN
cord-004738-vnz15x84	136	13	complex	complex	NN
cord-004738-vnz15x84	136	14	to	to	TO
cord-004738-vnz15x84	136	15	initiate	initiate	VB
cord-004738-vnz15x84	136	16	viral	viral	JJ
cord-004738-vnz15x84	136	17	negative	negative	JJ
cord-004738-vnz15x84	136	18	-	-	HYPH
cord-004738-vnz15x84	136	19	strand	strand	NN
cord-004738-vnz15x84	136	20	RNA	RNA	NNP
cord-004738-vnz15x84	136	21	synthesis	synthesis	NN
cord-004738-vnz15x84	136	22	.	.	.
cord-004738-vnz15x84	137	1	As	as	IN
cord-004738-vnz15x84	137	2	discussed	discuss	VBN
cord-004738-vnz15x84	137	3	in	in	IN
cord-004738-vnz15x84	137	4	the	the	DT
cord-004738-vnz15x84	137	5	introduction	introduction	NN
cord-004738-vnz15x84	137	6	,	,	,
cord-004738-vnz15x84	137	7	previous	previous	JJ
cord-004738-vnz15x84	137	8	experimental	experimental	JJ
cord-004738-vnz15x84	137	9	evidence	evidence	NN
cord-004738-vnz15x84	137	10	suggested	suggest	VBD
cord-004738-vnz15x84	137	11	that	that	IN
cord-004738-vnz15x84	137	12	P200	P200	NNP
cord-004738-vnz15x84	137	13	initiates	initiate	VBZ
cord-004738-vnz15x84	137	14	the	the	DT
cord-004738-vnz15x84	137	15	synthesis	synthesis	NN
cord-004738-vnz15x84	137	16	of	of	IN
cord-004738-vnz15x84	137	17	viral	viral	JJ
cord-004738-vnz15x84	137	18	negative	negative	JJ
cord-004738-vnz15x84	137	19	-	-	HYPH
cord-004738-vnz15x84	137	20	strand	strand	NN
cord-004738-vnz15x84	137	21	RNA	RNA	NNP
cord-004738-vnz15x84	137	22	,	,	,
cord-004738-vnz15x84	137	23	and	and	CC
cord-004738-vnz15x84	137	24	its	-PRON-	PRP$
cord-004738-vnz15x84	137	25	cleavage	cleavage	NN
cord-004738-vnz15x84	137	26	into	into	IN
cord-004738-vnz15x84	137	27	P150	P150	NNP
cord-004738-vnz15x84	137	28	and	and	CC
cord-004738-vnz15x84	137	29	P90	P90	NNP
cord-004738-vnz15x84	137	30	plays	play	VBZ
cord-004738-vnz15x84	137	31	a	a	DT
cord-004738-vnz15x84	137	32	critical	critical	JJ
cord-004738-vnz15x84	137	33	role	role	NN
cord-004738-vnz15x84	137	34	in	in	IN
cord-004738-vnz15x84	137	35	switching	switch	VBG
cord-004738-vnz15x84	137	36	the	the	DT
cord-004738-vnz15x84	137	37	replication	replication	NN
cord-004738-vnz15x84	137	38	complex	complex	JJ
cord-004738-vnz15x84	137	39	to	to	IN
cord-004738-vnz15x84	137	40	the	the	DT
cord-004738-vnz15x84	137	41	positive	positive	JJ
cord-004738-vnz15x84	137	42	RNA	RNA	NNP
cord-004738-vnz15x84	137	43	synthesis	synthesis	NN
cord-004738-vnz15x84	137	44	mode	mode	NN
cord-004738-vnz15x84	137	45	[	[	-LRB-
cord-004738-vnz15x84	137	46	8	8	CD
cord-004738-vnz15x84	137	47	]	]	-RRB-
cord-004738-vnz15x84	137	48	.	.	.
cord-004738-vnz15x84	138	1	In	in	IN
cord-004738-vnz15x84	138	2	this	this	DT
cord-004738-vnz15x84	138	3	context	context	NN
cord-004738-vnz15x84	138	4	,	,	,
cord-004738-vnz15x84	138	5	our	-PRON-	PRP$
cord-004738-vnz15x84	138	6	results	result	NNS
cord-004738-vnz15x84	138	7	leads	lead	VBZ
cord-004738-vnz15x84	138	8	us	-PRON-	PRP
cord-004738-vnz15x84	138	9	to	to	TO
cord-004738-vnz15x84	138	10	speculate	speculate	VB
cord-004738-vnz15x84	138	11	that	that	IN
cord-004738-vnz15x84	138	12	perhaps	perhaps	RB
cord-004738-vnz15x84	138	13	the	the	DT
cord-004738-vnz15x84	138	14	P150-protease	p150-protease	JJ
cord-004738-vnz15x84	138	15	transactivity	transactivity	NN
cord-004738-vnz15x84	138	16	requirement	requirement	NN
cord-004738-vnz15x84	138	17	of	of	IN
cord-004738-vnz15x84	138	18	the	the	DT
cord-004738-vnz15x84	138	19	P200	p200	NN
cord-004738-vnz15x84	138	20	sequence	sequence	NN
cord-004738-vnz15x84	138	21	within	within	IN
cord-004738-vnz15x84	138	22	its	-PRON-	PRP$
cord-004738-vnz15x84	138	23	essential	essential	JJ
cord-004738-vnz15x84	138	24	cis	cis	JJ
cord-004738-vnz15x84	138	25	-	-	HYPH
cord-004738-vnz15x84	138	26	activity	activity	NN
cord-004738-vnz15x84	138	27	region	region	NN
cord-004738-vnz15x84	138	28	(	(	-LRB-
cord-004738-vnz15x84	138	29	illustrated	illustrate	VBN
cord-004738-vnz15x84	138	30	in	in	IN
cord-004738-vnz15x84	138	31	Fig	Fig	NNP
cord-004738-vnz15x84	138	32	.	.	.
cord-004738-vnz15x84	139	1	4	4	LS
cord-004738-vnz15x84	139	2	)	)	-RRB-
cord-004738-vnz15x84	139	3	could	could	MD
cord-004738-vnz15x84	139	4	transiently	transiently	RB
cord-004738-vnz15x84	139	5	stall	stall	VB
cord-004738-vnz15x84	139	6	the	the	DT
cord-004738-vnz15x84	139	7	P200	P200	NNP
cord-004738-vnz15x84	139	8	cis	cis	NN
cord-004738-vnz15x84	139	9	-	-	HYPH
cord-004738-vnz15x84	139	10	protease	protease	NN
cord-004738-vnz15x84	139	11	activity	activity	NN
cord-004738-vnz15x84	139	12	,	,	,
cord-004738-vnz15x84	139	13	provided	provide	VBN
cord-004738-vnz15x84	139	14	that	that	IN
cord-004738-vnz15x84	139	15	P150	P150	NNP
cord-004738-vnz15x84	139	16	binds	bind	VBZ
cord-004738-vnz15x84	139	17	to	to	IN
cord-004738-vnz15x84	139	18	P200	p200	NN
cord-004738-vnz15x84	139	19	in	in	IN
cord-004738-vnz15x84	139	20	this	this	DT
cord-004738-vnz15x84	139	21	region	region	NN
cord-004738-vnz15x84	139	22	,	,	,
cord-004738-vnz15x84	139	23	to	to	TO
cord-004738-vnz15x84	139	24	allow	allow	VB
cord-004738-vnz15x84	139	25	the	the	DT
cord-004738-vnz15x84	139	26	negative	negative	JJ
cord-004738-vnz15x84	139	27	-	-	HYPH
cord-004738-vnz15x84	139	28	strand	strand	NN
cord-004738-vnz15x84	139	29	RNA	RNA	NNP
cord-004738-vnz15x84	139	30	synthesis	synthesis	NN
cord-004738-vnz15x84	139	31	to	to	TO
cord-004738-vnz15x84	139	32	occur	occur	VB
cord-004738-vnz15x84	139	33	.	.	.
cord-004738-vnz15x84	140	1	This	this	DT
cord-004738-vnz15x84	140	2	certainly	certainly	RB
cord-004738-vnz15x84	140	3	is	be	VBZ
cord-004738-vnz15x84	140	4	a	a	DT
cord-004738-vnz15x84	140	5	verifiable	verifiable	JJ
cord-004738-vnz15x84	140	6	future	future	JJ
cord-004738-vnz15x84	140	7	experimental	experimental	JJ
cord-004738-vnz15x84	140	8	direction	direction	NN
cord-004738-vnz15x84	140	9	to	to	TO
cord-004738-vnz15x84	140	10	capture	capture	VB
cord-004738-vnz15x84	140	11	viral	viral	JJ
cord-004738-vnz15x84	140	12	events	event	NNS
cord-004738-vnz15x84	140	13	that	that	WDT
cord-004738-vnz15x84	140	14	further	further	RB
cord-004738-vnz15x84	140	15	enhance	enhance	VB
cord-004738-vnz15x84	140	16	our	-PRON-	PRP$
cord-004738-vnz15x84	140	17	understanding	understanding	NN
cord-004738-vnz15x84	140	18	of	of	IN
cord-004738-vnz15x84	140	19	the	the	DT
cord-004738-vnz15x84	140	20	rubivirus	rubivirus	NN
cord-004738-vnz15x84	140	21	proteases	protease	NNS
cord-004738-vnz15x84	140	22	.	.	.
cord-004738-vnz15x84	141	1	Role	role	NN
cord-004738-vnz15x84	141	2	of	of	IN
cord-004738-vnz15x84	141	3	calreticulin	calreticulin	NNP
cord-004738-vnz15x84	141	4	in	in	IN
cord-004738-vnz15x84	141	5	rubella	rubella	NN
cord-004738-vnz15x84	141	6	virus	virus	NN
cord-004738-vnz15x84	141	7	replication	replication	NN
cord-004738-vnz15x84	141	8	Rubella	Rubella	NNP
cord-004738-vnz15x84	141	9	virus	virus	NN
cord-004738-vnz15x84	141	10	Characterization	characterization	NN
cord-004738-vnz15x84	141	11	of	of	IN
cord-004738-vnz15x84	141	12	the	the	DT
cord-004738-vnz15x84	141	13	rubella	rubella	NN
cord-004738-vnz15x84	141	14	virus	virus	NN
cord-004738-vnz15x84	141	15	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	141	16	protease	protease	NN
cord-004738-vnz15x84	141	17	domain	domain	NN
cord-004738-vnz15x84	141	18	and	and	CC
cord-004738-vnz15x84	141	19	its	-PRON-	PRP$
cord-004738-vnz15x84	141	20	cleavage	cleavage	NN
cord-004738-vnz15x84	141	21	site	site	NN
cord-004738-vnz15x84	141	22	Molecular	molecular	JJ
cord-004738-vnz15x84	141	23	biology	biology	NN
cord-004738-vnz15x84	141	24	of	of	IN
cord-004738-vnz15x84	141	25	rubella	rubella	NN
cord-004738-vnz15x84	141	26	virus	virus	NN
cord-004738-vnz15x84	141	27	Putative	putative	JJ
cord-004738-vnz15x84	141	28	papain	papain	NN
cord-004738-vnz15x84	141	29	-	-	HYPH
cord-004738-vnz15x84	141	30	related	relate	VBN
cord-004738-vnz15x84	141	31	thiol	thiol	NN
cord-004738-vnz15x84	141	32	proteases	protease	NNS
cord-004738-vnz15x84	141	33	of	of	IN
cord-004738-vnz15x84	141	34	positive	positive	JJ
cord-004738-vnz15x84	141	35	-	-	HYPH
cord-004738-vnz15x84	141	36	strand	strand	NN
cord-004738-vnz15x84	141	37	RNA	RNA	NNP
cord-004738-vnz15x84	141	38	viruses	virus	NNS
cord-004738-vnz15x84	141	39	.	.	.
cord-004738-vnz15x84	142	1	Identification	identification	NN
cord-004738-vnz15x84	142	2	of	of	IN
cord-004738-vnz15x84	142	3	rubi	rubi	NNP
cord-004738-vnz15x84	142	4	-	-	HYPH
cord-004738-vnz15x84	142	5	and	and	CC
cord-004738-vnz15x84	142	6	aphthovirus	aphthovirus	NNP
cord-004738-vnz15x84	142	7	proteases	protease	NNS
cord-004738-vnz15x84	142	8	and	and	CC
cord-004738-vnz15x84	142	9	delineation	delineation	NN
cord-004738-vnz15x84	142	10	of	of	IN
cord-004738-vnz15x84	142	11	a	a	DT
cord-004738-vnz15x84	142	12	novel	novel	JJ
cord-004738-vnz15x84	142	13	conserved	conserve	VBN
cord-004738-vnz15x84	142	14	domain	domain	NN
cord-004738-vnz15x84	142	15	associated	associate	VBN
cord-004738-vnz15x84	142	16	with	with	IN
cord-004738-vnz15x84	142	17	proteases	protease	NNS
cord-004738-vnz15x84	142	18	of	of	IN
cord-004738-vnz15x84	142	19	rubi-	rubi-	NN
cord-004738-vnz15x84	142	20	,	,	,
cord-004738-vnz15x84	142	21	alpha	alpha	NN
cord-004738-vnz15x84	142	22	-	-	HYPH
cord-004738-vnz15x84	142	23	and	and	CC
cord-004738-vnz15x84	142	24	coronaviruses	coronaviruse	NNS
cord-004738-vnz15x84	142	25	Evolution	evolution	NN
cord-004738-vnz15x84	142	26	and	and	CC
cord-004738-vnz15x84	142	27	taxonomy	taxonomy	NN
cord-004738-vnz15x84	142	28	of	of	IN
cord-004738-vnz15x84	142	29	positive	positive	JJ
cord-004738-vnz15x84	142	30	-	-	HYPH
cord-004738-vnz15x84	142	31	strand	strand	NN
cord-004738-vnz15x84	142	32	RNA	RNA	NNP
cord-004738-vnz15x84	142	33	viruses	virus	NNS
cord-004738-vnz15x84	142	34	:	:	:
cord-004738-vnz15x84	142	35	implications	implication	NNS
cord-004738-vnz15x84	142	36	of	of	IN
cord-004738-vnz15x84	142	37	comparative	comparative	JJ
cord-004738-vnz15x84	142	38	analysis	analysis	NN
cord-004738-vnz15x84	142	39	of	of	IN
cord-004738-vnz15x84	142	40	amino	amino	NN
cord-004738-vnz15x84	142	41	acid	acid	NN
cord-004738-vnz15x84	142	42	sequences	sequence	NNS
cord-004738-vnz15x84	142	43	Viral	Viral	NNP
cord-004738-vnz15x84	142	44	proteinases	proteinases	NNP
cord-004738-vnz15x84	142	45	Rubella	Rubella	NNP
cord-004738-vnz15x84	142	46	virus	virus	NN
cord-004738-vnz15x84	142	47	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	142	48	protein	protein	NN
cord-004738-vnz15x84	142	49	protease	protease	NN
cord-004738-vnz15x84	142	50	domains	domain	NNS
cord-004738-vnz15x84	142	51	involved	involve	VBN
cord-004738-vnz15x84	142	52	in	in	IN
cord-004738-vnz15x84	142	53	trans	trans	NNP
cord-004738-vnz15x84	142	54	-	-	:
cord-004738-vnz15x84	142	55	and	and	CC
cord-004738-vnz15x84	142	56	cis	cis	NNP
cord-004738-vnz15x84	142	57	-	-	HYPH
cord-004738-vnz15x84	142	58	cleavage	cleavage	NN
cord-004738-vnz15x84	142	59	activities	activity	NNS
cord-004738-vnz15x84	143	1	The	the	DT
cord-004738-vnz15x84	143	2	rubella	rubella	NN
cord-004738-vnz15x84	143	3	virus	virus	NN
cord-004738-vnz15x84	143	4	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	143	5	protease	protease	NN
cord-004738-vnz15x84	143	6	requires	require	VBZ
cord-004738-vnz15x84	143	7	divalent	divalent	JJ
cord-004738-vnz15x84	143	8	cations	cation	NNS
cord-004738-vnz15x84	143	9	for	for	IN
cord-004738-vnz15x84	143	10	activity	activity	NN
cord-004738-vnz15x84	143	11	and	and	CC
cord-004738-vnz15x84	143	12	functions	function	NNS
cord-004738-vnz15x84	143	13	in	in	IN
cord-004738-vnz15x84	143	14	trans	trans	NNP
cord-004738-vnz15x84	143	15	Characterization	Characterization	NNP
cord-004738-vnz15x84	143	16	of	of	IN
cord-004738-vnz15x84	143	17	the	the	DT
cord-004738-vnz15x84	143	18	zinc	zinc	NN
cord-004738-vnz15x84	143	19	binding	bind	VBG
cord-004738-vnz15x84	143	20	activity	activity	NN
cord-004738-vnz15x84	143	21	of	of	IN
cord-004738-vnz15x84	143	22	the	the	DT
cord-004738-vnz15x84	143	23	rubella	rubella	NN
cord-004738-vnz15x84	143	24	virus	virus	NN
cord-004738-vnz15x84	143	25	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	143	26	protease	protease	NN
cord-004738-vnz15x84	143	27	Expression	expression	NN
cord-004738-vnz15x84	143	28	of	of	IN
cord-004738-vnz15x84	143	29	the	the	DT
cord-004738-vnz15x84	143	30	rubella	rubella	NN
cord-004738-vnz15x84	143	31	virus	virus	NN
cord-004738-vnz15x84	143	32	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	143	33	protein	protein	NN
cord-004738-vnz15x84	143	34	ORF	ORF	NNP
cord-004738-vnz15x84	143	35	and	and	CC
cord-004738-vnz15x84	143	36	demonstration	demonstration	NN
cord-004738-vnz15x84	143	37	of	of	IN
cord-004738-vnz15x84	143	38	proteolytic	proteolytic	JJ
cord-004738-vnz15x84	143	39	processing	processing	NN
cord-004738-vnz15x84	144	1	The	the	DT
cord-004738-vnz15x84	144	2	N	n	CD
cord-004738-vnz15x84	144	3	-	-	HYPH
cord-004738-vnz15x84	144	4	terminal	terminal	JJ
cord-004738-vnz15x84	144	5	conserved	conserved	JJ
cord-004738-vnz15x84	144	6	domain	domain	NN
cord-004738-vnz15x84	144	7	of	of	IN
cord-004738-vnz15x84	144	8	rubella	rubella	NN
cord-004738-vnz15x84	144	9	virus	virus	NN
cord-004738-vnz15x84	144	10	capsid	capsid	NN
cord-004738-vnz15x84	144	11	interacts	interact	VBZ
cord-004738-vnz15x84	144	12	with	with	IN
cord-004738-vnz15x84	144	13	the	the	DT
cord-004738-vnz15x84	144	14	C	C	NNP
cord-004738-vnz15x84	144	15	-	-	HYPH
cord-004738-vnz15x84	144	16	terminal	terminal	NN
cord-004738-vnz15x84	144	17	region	region	NN
cord-004738-vnz15x84	144	18	of	of	IN
cord-004738-vnz15x84	144	19	cellular	cellular	JJ
cord-004738-vnz15x84	144	20	p32	p32	NN
cord-004738-vnz15x84	144	21	and	and	CC
cord-004738-vnz15x84	144	22	overexpression	overexpression	NN
cord-004738-vnz15x84	144	23	of	of	IN
cord-004738-vnz15x84	144	24	p32	p32	NN
cord-004738-vnz15x84	144	25	enhances	enhance	VBZ
cord-004738-vnz15x84	144	26	the	the	DT
cord-004738-vnz15x84	144	27	viral	viral	JJ
cord-004738-vnz15x84	144	28	infectivity	infectivity	NN
cord-004738-vnz15x84	144	29	Conservation	Conservation	NNP
cord-004738-vnz15x84	144	30	of	of	IN
cord-004738-vnz15x84	144	31	the	the	DT
cord-004738-vnz15x84	144	32	putative	putative	JJ
cord-004738-vnz15x84	144	33	methyltransferase	methyltransferase	NN
cord-004738-vnz15x84	144	34	domain	domain	NN
cord-004738-vnz15x84	144	35	:	:	:
cord-004738-vnz15x84	144	36	a	a	DT
cord-004738-vnz15x84	144	37	hallmark	hallmark	NN
cord-004738-vnz15x84	144	38	of	of	IN
cord-004738-vnz15x84	144	39	the	the	DT
cord-004738-vnz15x84	144	40	'	'	``
cord-004738-vnz15x84	144	41	Sindbis	Sindbis	NNP
cord-004738-vnz15x84	144	42	-	-	HYPH
cord-004738-vnz15x84	144	43	like	like	JJ
cord-004738-vnz15x84	144	44	'	'	''
cord-004738-vnz15x84	144	45	supergroup	supergroup	NN
cord-004738-vnz15x84	144	46	of	of	IN
cord-004738-vnz15x84	144	47	positive	positive	JJ
cord-004738-vnz15x84	144	48	-	-	HYPH
cord-004738-vnz15x84	144	49	strand	strand	NN
cord-004738-vnz15x84	144	50	RNA	RNA	NNP
cord-004738-vnz15x84	144	51	viruses	virus	NNS
cord-004738-vnz15x84	144	52	Virus	virus	NN
cord-004738-vnz15x84	144	53	-	-	HYPH
cord-004738-vnz15x84	144	54	encoded	encode	VBN
cord-004738-vnz15x84	144	55	proteinases	proteinase	NNS
cord-004738-vnz15x84	144	56	of	of	IN
cord-004738-vnz15x84	144	57	the	the	DT
cord-004738-vnz15x84	144	58	Togaviridae	Togaviridae	NNP
cord-004738-vnz15x84	144	59	Expression	Expression	NNP
cord-004738-vnz15x84	144	60	of	of	IN
cord-004738-vnz15x84	144	61	murine	murine	JJ
cord-004738-vnz15x84	144	62	coronavirus	coronavirus	NN
cord-004738-vnz15x84	144	63	recombinant	recombinant	JJ
cord-004738-vnz15x84	144	64	papain	papain	NN
cord-004738-vnz15x84	144	65	-	-	HYPH
cord-004738-vnz15x84	144	66	like	like	JJ
cord-004738-vnz15x84	144	67	proteinase	proteinase	NN
cord-004738-vnz15x84	144	68	:	:	:
cord-004738-vnz15x84	144	69	efficient	efficient	JJ
cord-004738-vnz15x84	144	70	cleavage	cleavage	NN
cord-004738-vnz15x84	144	71	is	be	VBZ
cord-004738-vnz15x84	144	72	dependent	dependent	JJ
cord-004738-vnz15x84	144	73	on	on	IN
cord-004738-vnz15x84	144	74	the	the	DT
cord-004738-vnz15x84	144	75	lengths	length	NNS
cord-004738-vnz15x84	144	76	of	of	IN
cord-004738-vnz15x84	144	77	both	both	CC
cord-004738-vnz15x84	144	78	the	the	DT
cord-004738-vnz15x84	144	79	substrate	substrate	NN
cord-004738-vnz15x84	144	80	and	and	CC
cord-004738-vnz15x84	144	81	the	the	DT
cord-004738-vnz15x84	144	82	proteinase	proteinase	NN
cord-004738-vnz15x84	144	83	polypeptides	polypeptide	NNS
cord-004738-vnz15x84	144	84	Site	site	NN
cord-004738-vnz15x84	144	85	-	-	HYPH
cord-004738-vnz15x84	144	86	specific	specific	JJ
cord-004738-vnz15x84	144	87	protease	protease	NN
cord-004738-vnz15x84	144	88	activity	activity	NN
cord-004738-vnz15x84	144	89	of	of	IN
cord-004738-vnz15x84	144	90	the	the	DT
cord-004738-vnz15x84	144	91	carboxyl	carboxyl	NN
cord-004738-vnz15x84	144	92	-	-	HYPH
cord-004738-vnz15x84	144	93	terminal	terminal	NN
cord-004738-vnz15x84	144	94	domain	domain	NN
cord-004738-vnz15x84	144	95	of	of	IN
cord-004738-vnz15x84	144	96	Semliki	Semliki	NNP
cord-004738-vnz15x84	144	97	Forest	Forest	NNP
cord-004738-vnz15x84	144	98	virus	virus	NN
cord-004738-vnz15x84	144	99	replicase	replicase	NN
cord-004738-vnz15x84	144	100	protein	protein	NN
cord-004738-vnz15x84	145	1	nsP2	nsP2	NNP
cord-004738-vnz15x84	145	2	Rescue	Rescue	NNP
cord-004738-vnz15x84	145	3	of	of	IN
cord-004738-vnz15x84	145	4	rubella	rubella	NN
cord-004738-vnz15x84	145	5	virus	virus	NN
cord-004738-vnz15x84	145	6	replication	replication	NN
cord-004738-vnz15x84	145	7	-	-	HYPH
cord-004738-vnz15x84	145	8	defective	defective	JJ
cord-004738-vnz15x84	145	9	mutants	mutant	NNS
cord-004738-vnz15x84	145	10	using	use	VBG
cord-004738-vnz15x84	145	11	vaccinia	vaccinia	NN
cord-004738-vnz15x84	145	12	virus	virus	NN
cord-004738-vnz15x84	145	13	recombinant	recombinant	JJ
cord-004738-vnz15x84	145	14	expressing	express	VBG
cord-004738-vnz15x84	145	15	rubella	rubella	NN
cord-004738-vnz15x84	145	16	virus	virus	NN
cord-004738-vnz15x84	145	17	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	145	18	proteins	protein	NNS
cord-004738-vnz15x84	145	19	Proteolytic	Proteolytic	NNP
cord-004738-vnz15x84	145	20	processing	processing	NN
cord-004738-vnz15x84	145	21	of	of	IN
cord-004738-vnz15x84	145	22	rubella	rubella	NN
cord-004738-vnz15x84	145	23	virus	virus	NN
cord-004738-vnz15x84	145	24	nonstructural	nonstructural	JJ
cord-004738-vnz15x84	145	25	proteins	protein	NNS
cord-004738-vnz15x84	146	1	We	-PRON-	PRP
cord-004738-vnz15x84	146	2	thank	thank	VBP
cord-004738-vnz15x84	146	3	Dr.	Dr.	NNP
cord-004738-vnz15x84	146	4	Shirley	Shirley	NNP
cord-004738-vnz15x84	146	5	Gillam	Gillam	NNP
cord-004738-vnz15x84	146	6	for	for	IN
cord-004738-vnz15x84	146	7	providing	provide	VBG
cord-004738-vnz15x84	146	8	M33	M33	NNP
cord-004738-vnz15x84	146	9	plasmid	plasmid	NN
cord-004738-vnz15x84	146	10	constructs	construct	NNS
cord-004738-vnz15x84	146	11	pBRM33-C1152S	pBRM33-C1152S	NNP
cord-004738-vnz15x84	146	12	and	and	CC
cord-004738-vnz15x84	146	13	pBRM33-G1301S	pbrm33-g1301s	NN
cord-004738-vnz15x84	146	14	that	that	WDT
cord-004738-vnz15x84	146	15	were	be	VBD
cord-004738-vnz15x84	146	16	used	use	VBN
cord-004738-vnz15x84	146	17	in	in	IN
cord-004738-vnz15x84	146	18	the	the	DT
cord-004738-vnz15x84	146	19	study	study	NN
cord-004738-vnz15x84	146	20	as	as	IN
cord-004738-vnz15x84	146	21	PCR	PCR	NNP
cord-004738-vnz15x84	146	22	templates	template	NNS
cord-004738-vnz15x84	146	23	to	to	TO
cord-004738-vnz15x84	146	24	generate	generate	VB
cord-004738-vnz15x84	146	25	the	the	DT
cord-004738-vnz15x84	146	26	protease	protease	NN
cord-004738-vnz15x84	146	27	and	and	CC
cord-004738-vnz15x84	146	28	substrate	substrate	NN
cord-004738-vnz15x84	146	29	clones	clone	NNS
cord-004738-vnz15x84	146	30	.	.	.
cord-004738-vnz15x84	147	1	We	-PRON-	PRP
cord-004738-vnz15x84	147	2	thank	thank	VBP
cord-004738-vnz15x84	147	3	Drs	Drs	NNP
cord-004738-vnz15x84	147	4	.	.	.
cord-004738-vnz15x84	148	1	KVK	KVK	NNP
cord-004738-vnz15x84	148	2	Mohan	Mohan	NNP
cord-004738-vnz15x84	148	3	and	and	CC
cord-004738-vnz15x84	148	4	Ye	Ye	NNP
cord-004738-vnz15x84	148	5	,	,	,
cord-004738-vnz15x84	148	6	CBER	CBER	NNP
cord-004738-vnz15x84	148	7	for	for	IN
cord-004738-vnz15x84	148	8	helpful	helpful	JJ
cord-004738-vnz15x84	148	9	critiques	critique	NNS
cord-004738-vnz15x84	148	10	.	.	.
cord-004738-vnz15x84	149	1	HHC	HHC	NNP
cord-004738-vnz15x84	149	2	and	and	CC
cord-004738-vnz15x84	149	3	CJS	CJS	NNP
cord-004738-vnz15x84	149	4	are	be	VBP
cord-004738-vnz15x84	149	5	supported	support	VBN
cord-004738-vnz15x84	149	6	by	by	IN
cord-004738-vnz15x84	149	7	ORISE	ORISE	NNP
cord-004738-vnz15x84	149	8	postdoctoral	postdoctoral	JJ
cord-004738-vnz15x84	149	9	fellowships	fellowship	NNS
cord-004738-vnz15x84	149	10	.	.	.