id sid tid token lemma pos cord-308950-bl83r4v3 1 1 key key NN cord-308950-bl83r4v3 1 2 : : : cord-308950-bl83r4v3 1 3 cord-308950-bl83r4v3 cord-308950-bl83r4v3 NN cord-308950-bl83r4v3 1 4 authors author NNS cord-308950-bl83r4v3 1 5 : : : cord-308950-bl83r4v3 1 6 Miguel Miguel NNP cord-308950-bl83r4v3 1 7 , , , cord-308950-bl83r4v3 1 8 B. B. NNP cord-308950-bl83r4v3 1 9 ; ; : cord-308950-bl83r4v3 1 10 Pharr Pharr NNP cord-308950-bl83r4v3 1 11 , , , cord-308950-bl83r4v3 1 12 G. G. NNP cord-308950-bl83r4v3 1 13 T. T. NNP cord-308950-bl83r4v3 1 14 ; ; : cord-308950-bl83r4v3 1 15 Wang Wang NNP cord-308950-bl83r4v3 1 16 , , , cord-308950-bl83r4v3 1 17 C. C. NNP cord-308950-bl83r4v3 1 18 title title NN cord-308950-bl83r4v3 1 19 : : : cord-308950-bl83r4v3 2 1 The the DT cord-308950-bl83r4v3 2 2 role role NN cord-308950-bl83r4v3 2 3 of of IN cord-308950-bl83r4v3 2 4 feline feline JJ cord-308950-bl83r4v3 2 5 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 2 6 N N NNP cord-308950-bl83r4v3 2 7 as as IN cord-308950-bl83r4v3 2 8 a a DT cord-308950-bl83r4v3 2 9 receptor receptor NN cord-308950-bl83r4v3 2 10 for for IN cord-308950-bl83r4v3 2 11 infectious infectious JJ cord-308950-bl83r4v3 2 12 bronchitis bronchitis NN cord-308950-bl83r4v3 2 13 virus virus NN cord-308950-bl83r4v3 2 14 : : : cord-308950-bl83r4v3 2 15 Brief brief JJ cord-308950-bl83r4v3 2 16 Review Review NNP cord-308950-bl83r4v3 2 17 date date NN cord-308950-bl83r4v3 2 18 : : : cord-308950-bl83r4v3 2 19 2002 2002 CD cord-308950-bl83r4v3 2 20 journal journal NN cord-308950-bl83r4v3 2 21 : : : cord-308950-bl83r4v3 3 1 Arch arch JJ cord-308950-bl83r4v3 3 2 Virol Virol NNP cord-308950-bl83r4v3 3 3 DOI doi NN cord-308950-bl83r4v3 3 4 : : : cord-308950-bl83r4v3 4 1 10.1007 10.1007 CD cord-308950-bl83r4v3 4 2 / / SYM cord-308950-bl83r4v3 4 3 s00705 s00705 NNP cord-308950-bl83r4v3 4 4 - - HYPH cord-308950-bl83r4v3 4 5 002 002 CD cord-308950-bl83r4v3 4 6 - - HYPH cord-308950-bl83r4v3 4 7 0888 0888 CD cord-308950-bl83r4v3 4 8 - - SYM cord-308950-bl83r4v3 4 9 1 1 CD cord-308950-bl83r4v3 4 10 sha sha NN cord-308950-bl83r4v3 4 11 : : : cord-308950-bl83r4v3 4 12 374320be9c08609336a2ff4cb595adfe8bd4427d 374320be9c08609336a2ff4cb595adfe8bd4427d CD cord-308950-bl83r4v3 4 13 doc_id doc_id CD cord-308950-bl83r4v3 4 14 : : : cord-308950-bl83r4v3 4 15 308950 308950 CD cord-308950-bl83r4v3 4 16 cord_uid cord_uid NNS cord-308950-bl83r4v3 4 17 : : : cord-308950-bl83r4v3 4 18 bl83r4v3 bl83r4v3 NNP cord-308950-bl83r4v3 4 19 Feline feline JJ cord-308950-bl83r4v3 4 20 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 4 21 N N NNP cord-308950-bl83r4v3 4 22 ( ( -LRB- cord-308950-bl83r4v3 4 23 fAPN fapn NN cord-308950-bl83r4v3 4 24 ) ) -RRB- cord-308950-bl83r4v3 4 25 has have VBZ cord-308950-bl83r4v3 4 26 been be VBN cord-308950-bl83r4v3 4 27 shown show VBN cord-308950-bl83r4v3 4 28 to to TO cord-308950-bl83r4v3 4 29 serve serve VB cord-308950-bl83r4v3 4 30 as as IN cord-308950-bl83r4v3 4 31 a a DT cord-308950-bl83r4v3 4 32 receptor receptor NN cord-308950-bl83r4v3 4 33 for for IN cord-308950-bl83r4v3 4 34 feline feline JJ cord-308950-bl83r4v3 4 35 , , , cord-308950-bl83r4v3 4 36 canine canine JJ cord-308950-bl83r4v3 4 37 , , , cord-308950-bl83r4v3 4 38 porcine porcine JJ cord-308950-bl83r4v3 4 39 and and CC cord-308950-bl83r4v3 4 40 human human JJ cord-308950-bl83r4v3 4 41 coronaviruses coronaviruse NNS cord-308950-bl83r4v3 4 42 . . . cord-308950-bl83r4v3 5 1 Our -PRON- PRP$ cord-308950-bl83r4v3 5 2 objective objective NN cord-308950-bl83r4v3 5 3 was be VBD cord-308950-bl83r4v3 5 4 to to TO cord-308950-bl83r4v3 5 5 determine determine VB cord-308950-bl83r4v3 5 6 if if IN cord-308950-bl83r4v3 5 7 fAPN fapn PRP cord-308950-bl83r4v3 5 8 can can MD cord-308950-bl83r4v3 5 9 serve serve VB cord-308950-bl83r4v3 5 10 as as IN cord-308950-bl83r4v3 5 11 a a DT cord-308950-bl83r4v3 5 12 receptor receptor NN cord-308950-bl83r4v3 5 13 for for IN cord-308950-bl83r4v3 5 14 infectious infectious JJ cord-308950-bl83r4v3 5 15 bronchitis bronchitis NN cord-308950-bl83r4v3 5 16 virus virus NN cord-308950-bl83r4v3 5 17 ( ( -LRB- cord-308950-bl83r4v3 5 18 IBV IBV NNP cord-308950-bl83r4v3 5 19 ) ) -RRB- cord-308950-bl83r4v3 5 20 . . . cord-308950-bl83r4v3 6 1 Feline feline JJ cord-308950-bl83r4v3 6 2 kidney kidney NN cord-308950-bl83r4v3 6 3 cells cell NNS cord-308950-bl83r4v3 6 4 that that WDT cord-308950-bl83r4v3 6 5 express express VBP cord-308950-bl83r4v3 6 6 fAPN fapn NN cord-308950-bl83r4v3 6 7 and and CC cord-308950-bl83r4v3 6 8 hamster hamster NN cord-308950-bl83r4v3 6 9 kidney kidney NN cord-308950-bl83r4v3 6 10 fibroblasts fibroblast NNS cord-308950-bl83r4v3 6 11 that that WDT cord-308950-bl83r4v3 6 12 do do VBP cord-308950-bl83r4v3 6 13 not not RB cord-308950-bl83r4v3 6 14 express express VB cord-308950-bl83r4v3 6 15 fAPN fAPN NNP cord-308950-bl83r4v3 6 16 were be VBD cord-308950-bl83r4v3 6 17 inoculated inoculate VBN cord-308950-bl83r4v3 6 18 with with IN cord-308950-bl83r4v3 6 19 IBV IBV NNP cord-308950-bl83r4v3 6 20 and and CC cord-308950-bl83r4v3 6 21 monitored monitor VBD cord-308950-bl83r4v3 6 22 for for IN cord-308950-bl83r4v3 6 23 replication replication NN cord-308950-bl83r4v3 6 24 by by IN cord-308950-bl83r4v3 6 25 indirect indirect JJ cord-308950-bl83r4v3 6 26 fluorescent fluorescent NN cord-308950-bl83r4v3 6 27 assay assay NN cord-308950-bl83r4v3 6 28 and and CC cord-308950-bl83r4v3 6 29 confocal confocal JJ cord-308950-bl83r4v3 6 30 microscopy microscopy NNS cord-308950-bl83r4v3 6 31 and and CC cord-308950-bl83r4v3 6 32 in in IN cord-308950-bl83r4v3 6 33 chicken chicken NN cord-308950-bl83r4v3 6 34 embryonated embryonate VBN cord-308950-bl83r4v3 6 35 eggs egg NNS cord-308950-bl83r4v3 6 36 . . . cord-308950-bl83r4v3 7 1 The the DT cord-308950-bl83r4v3 7 2 results result NNS cord-308950-bl83r4v3 7 3 showed show VBD cord-308950-bl83r4v3 7 4 that that IN cord-308950-bl83r4v3 7 5 the the DT cord-308950-bl83r4v3 7 6 feline feline JJ cord-308950-bl83r4v3 7 7 cells cell NNS cord-308950-bl83r4v3 7 8 were be VBD cord-308950-bl83r4v3 7 9 permissive permissive JJ cord-308950-bl83r4v3 7 10 to to IN cord-308950-bl83r4v3 7 11 IBV IBV NNP cord-308950-bl83r4v3 7 12 but but CC cord-308950-bl83r4v3 7 13 the the DT cord-308950-bl83r4v3 7 14 hamster hamster NN cord-308950-bl83r4v3 7 15 cells cell NNS cord-308950-bl83r4v3 7 16 were be VBD cord-308950-bl83r4v3 7 17 not not RB cord-308950-bl83r4v3 7 18 . . . cord-308950-bl83r4v3 8 1 The the DT cord-308950-bl83r4v3 8 2 hamster hamster NN cord-308950-bl83r4v3 8 3 cells cell NNS cord-308950-bl83r4v3 8 4 became become VBD cord-308950-bl83r4v3 8 5 permissive permissive JJ cord-308950-bl83r4v3 8 6 to to IN cord-308950-bl83r4v3 8 7 IBV IBV NNP cord-308950-bl83r4v3 8 8 after after IN cord-308950-bl83r4v3 8 9 transfection transfection NN cord-308950-bl83r4v3 8 10 with with IN cord-308950-bl83r4v3 8 11 a a DT cord-308950-bl83r4v3 8 12 fAPN fapn FW cord-308950-bl83r4v3 8 13 cDNA cdna NN cord-308950-bl83r4v3 8 14 suggesting suggest VBG cord-308950-bl83r4v3 8 15 that that IN cord-308950-bl83r4v3 8 16 the the DT cord-308950-bl83r4v3 8 17 feline feline JJ cord-308950-bl83r4v3 8 18 APN APN NNP cord-308950-bl83r4v3 8 19 molecule molecule NN cord-308950-bl83r4v3 8 20 plays play VBZ cord-308950-bl83r4v3 8 21 a a DT cord-308950-bl83r4v3 8 22 role role NN cord-308950-bl83r4v3 8 23 in in IN cord-308950-bl83r4v3 8 24 IBV IBV NNP cord-308950-bl83r4v3 8 25 entry entry NN cord-308950-bl83r4v3 8 26 . . . cord-308950-bl83r4v3 9 1 Members member NNS cord-308950-bl83r4v3 9 2 of of IN cord-308950-bl83r4v3 9 3 the the DT cord-308950-bl83r4v3 9 4 family family NN cord-308950-bl83r4v3 9 5 Coronaviridae Coronaviridae NNP cord-308950-bl83r4v3 9 6 infect infect VBP cord-308950-bl83r4v3 9 7 a a DT cord-308950-bl83r4v3 9 8 wide wide JJ cord-308950-bl83r4v3 9 9 range range NN cord-308950-bl83r4v3 9 10 of of IN cord-308950-bl83r4v3 9 11 hosts host NNS cord-308950-bl83r4v3 9 12 and and CC cord-308950-bl83r4v3 9 13 have have VBP cord-308950-bl83r4v3 9 14 been be VBN cord-308950-bl83r4v3 9 15 classified classify VBN cord-308950-bl83r4v3 9 16 into into IN cord-308950-bl83r4v3 9 17 three three CD cord-308950-bl83r4v3 9 18 groups group NNS cord-308950-bl83r4v3 9 19 . . . cord-308950-bl83r4v3 10 1 One one CD cord-308950-bl83r4v3 10 2 major major JJ cord-308950-bl83r4v3 10 3 group group NN cord-308950-bl83r4v3 10 4 ( ( -LRB- cord-308950-bl83r4v3 10 5 group group NN cord-308950-bl83r4v3 10 6 I I NNP cord-308950-bl83r4v3 10 7 ) ) -RRB- cord-308950-bl83r4v3 10 8 was be VBD cord-308950-bl83r4v3 10 9 recently recently RB cord-308950-bl83r4v3 10 10 shown show VBN cord-308950-bl83r4v3 10 11 to to TO cord-308950-bl83r4v3 10 12 use use VB cord-308950-bl83r4v3 10 13 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 10 14 N N NNP cord-308950-bl83r4v3 10 15 ( ( -LRB- cord-308950-bl83r4v3 10 16 APN APN NNP cord-308950-bl83r4v3 10 17 ) ) -RRB- cord-308950-bl83r4v3 10 18 as as IN cord-308950-bl83r4v3 10 19 its -PRON- PRP$ cord-308950-bl83r4v3 10 20 cell cell NN cord-308950-bl83r4v3 10 21 surface surface NN cord-308950-bl83r4v3 10 22 receptor receptor NN cord-308950-bl83r4v3 10 23 [ [ -LRB- cord-308950-bl83r4v3 10 24 7 7 CD cord-308950-bl83r4v3 10 25 , , , cord-308950-bl83r4v3 10 26 9 9 CD cord-308950-bl83r4v3 10 27 , , , cord-308950-bl83r4v3 10 28 10 10 CD cord-308950-bl83r4v3 10 29 , , , cord-308950-bl83r4v3 10 30 11 11 CD cord-308950-bl83r4v3 10 31 , , , cord-308950-bl83r4v3 10 32 16 16 CD cord-308950-bl83r4v3 10 33 , , , cord-308950-bl83r4v3 10 34 21 21 CD cord-308950-bl83r4v3 10 35 - - SYM cord-308950-bl83r4v3 10 36 23 23 CD cord-308950-bl83r4v3 10 37 , , , cord-308950-bl83r4v3 10 38 28 28 CD cord-308950-bl83r4v3 10 39 , , , cord-308950-bl83r4v3 10 40 43 43 CD cord-308950-bl83r4v3 10 41 ] ] -RRB- cord-308950-bl83r4v3 10 42 . . . cord-308950-bl83r4v3 11 1 Aminopeptidase Aminopeptidase NNP cord-308950-bl83r4v3 11 2 N N NNP cord-308950-bl83r4v3 11 3 , , , cord-308950-bl83r4v3 11 4 also also RB cord-308950-bl83r4v3 11 5 called call VBD cord-308950-bl83r4v3 11 6 CD13 CD13 NNP cord-308950-bl83r4v3 11 7 in in IN cord-308950-bl83r4v3 11 8 humans human NNS cord-308950-bl83r4v3 11 9 [ [ -LRB- cord-308950-bl83r4v3 11 10 31 31 CD cord-308950-bl83r4v3 11 11 ] ] -RRB- cord-308950-bl83r4v3 11 12 , , , cord-308950-bl83r4v3 11 13 is be VBZ cord-308950-bl83r4v3 11 14 a a DT cord-308950-bl83r4v3 11 15 zinc zinc NN cord-308950-bl83r4v3 11 16 metalloprotease metalloprotease NN cord-308950-bl83r4v3 11 17 . . . cord-308950-bl83r4v3 12 1 It -PRON- PRP cord-308950-bl83r4v3 12 2 is be VBZ cord-308950-bl83r4v3 12 3 a a DT cord-308950-bl83r4v3 12 4 150-kDa 150-kda CD cord-308950-bl83r4v3 12 5 glycoprotein glycoprotein NN cord-308950-bl83r4v3 12 6 with with IN cord-308950-bl83r4v3 12 7 zinc zinc NN cord-308950-bl83r4v3 12 8 located locate VBN cord-308950-bl83r4v3 12 9 in in IN cord-308950-bl83r4v3 12 10 its -PRON- PRP$ cord-308950-bl83r4v3 12 11 globular globular JJ cord-308950-bl83r4v3 12 12 region region NN cord-308950-bl83r4v3 12 13 that that WDT cord-308950-bl83r4v3 12 14 is be VBZ cord-308950-bl83r4v3 12 15 believed believe VBN cord-308950-bl83r4v3 12 16 to to TO cord-308950-bl83r4v3 12 17 be be VB cord-308950-bl83r4v3 12 18 the the DT cord-308950-bl83r4v3 12 19 active active JJ cord-308950-bl83r4v3 12 20 enzymatic enzymatic JJ cord-308950-bl83r4v3 12 21 site site NN cord-308950-bl83r4v3 12 22 playing play VBG cord-308950-bl83r4v3 12 23 a a DT cord-308950-bl83r4v3 12 24 major major JJ cord-308950-bl83r4v3 12 25 role role NN cord-308950-bl83r4v3 12 26 in in IN cord-308950-bl83r4v3 12 27 the the DT cord-308950-bl83r4v3 12 28 cleaving cleaving NN cord-308950-bl83r4v3 12 29 of of IN cord-308950-bl83r4v3 12 30 peptides peptide NNS cord-308950-bl83r4v3 12 31 [ [ -LRB- cord-308950-bl83r4v3 12 32 1 1 CD cord-308950-bl83r4v3 12 33 , , , cord-308950-bl83r4v3 12 34 2 2 CD cord-308950-bl83r4v3 12 35 , , , cord-308950-bl83r4v3 12 36 12 12 CD cord-308950-bl83r4v3 12 37 , , , cord-308950-bl83r4v3 12 38 15 15 CD cord-308950-bl83r4v3 12 39 , , , cord-308950-bl83r4v3 12 40 33 33 CD cord-308950-bl83r4v3 12 41 ] ] -RRB- cord-308950-bl83r4v3 12 42 . . . cord-308950-bl83r4v3 13 1 APN APN NNP cord-308950-bl83r4v3 13 2 is be VBZ cord-308950-bl83r4v3 13 3 expressed express VBN cord-308950-bl83r4v3 13 4 on on IN cord-308950-bl83r4v3 13 5 the the DT cord-308950-bl83r4v3 13 6 plasma plasma NN cord-308950-bl83r4v3 13 7 membranes membrane NNS cord-308950-bl83r4v3 13 8 of of IN cord-308950-bl83r4v3 13 9 granulocytes granulocyte NNS cord-308950-bl83r4v3 13 10 [ [ -LRB- cord-308950-bl83r4v3 13 11 2 2 CD cord-308950-bl83r4v3 13 12 , , , cord-308950-bl83r4v3 13 13 36 36 CD cord-308950-bl83r4v3 13 14 ] ] -RRB- cord-308950-bl83r4v3 13 15 , , , cord-308950-bl83r4v3 13 16 lymphocytes lymphocyte NNS cord-308950-bl83r4v3 13 17 and and CC cord-308950-bl83r4v3 13 18 monocytes monocyte VBZ cord-308950-bl83r4v3 13 19 [ [ -LRB- cord-308950-bl83r4v3 13 20 26 26 CD cord-308950-bl83r4v3 13 21 , , , cord-308950-bl83r4v3 13 22 30 30 CD cord-308950-bl83r4v3 13 23 , , , cord-308950-bl83r4v3 13 24 31 31 CD cord-308950-bl83r4v3 13 25 , , , cord-308950-bl83r4v3 13 26 35 35 CD cord-308950-bl83r4v3 13 27 ] ] -RRB- cord-308950-bl83r4v3 13 28 . . . cord-308950-bl83r4v3 14 1 It -PRON- PRP cord-308950-bl83r4v3 14 2 is be VBZ cord-308950-bl83r4v3 14 3 also also RB cord-308950-bl83r4v3 14 4 expressed express VBN cord-308950-bl83r4v3 14 5 on on IN cord-308950-bl83r4v3 14 6 non non JJ cord-308950-bl83r4v3 14 7 - - JJ cord-308950-bl83r4v3 14 8 hematopoetic hematopoetic JJ cord-308950-bl83r4v3 14 9 tissue tissue NN cord-308950-bl83r4v3 14 10 including include VBG cord-308950-bl83r4v3 14 11 fibroblasts fibroblast NNS cord-308950-bl83r4v3 14 12 [ [ -LRB- cord-308950-bl83r4v3 14 13 35 35 CD cord-308950-bl83r4v3 14 14 ] ] -RRB- cord-308950-bl83r4v3 14 15 , , , cord-308950-bl83r4v3 14 16 synaptic synaptic JJ cord-308950-bl83r4v3 14 17 membranes membrane NNS cord-308950-bl83r4v3 14 18 in in IN cord-308950-bl83r4v3 14 19 the the DT cord-308950-bl83r4v3 14 20 central central JJ cord-308950-bl83r4v3 14 21 nervous nervous JJ cord-308950-bl83r4v3 14 22 system system NN cord-308950-bl83r4v3 14 23 [ [ -LRB- cord-308950-bl83r4v3 14 24 6 6 CD cord-308950-bl83r4v3 14 25 ] ] -RRB- cord-308950-bl83r4v3 14 26 , , , cord-308950-bl83r4v3 14 27 epithelial epithelial JJ cord-308950-bl83r4v3 14 28 cells cell NNS cord-308950-bl83r4v3 14 29 from from IN cord-308950-bl83r4v3 14 30 the the DT cord-308950-bl83r4v3 14 31 renal renal JJ cord-308950-bl83r4v3 14 32 proximal proximal JJ cord-308950-bl83r4v3 14 33 tubules tubule NNS cord-308950-bl83r4v3 14 34 and and CC cord-308950-bl83r4v3 14 35 the the DT cord-308950-bl83r4v3 14 36 intestinal intestinal JJ cord-308950-bl83r4v3 14 37 brush brush NN cord-308950-bl83r4v3 14 38 border border NN cord-308950-bl83r4v3 14 39 , , , cord-308950-bl83r4v3 14 40 and and CC cord-308950-bl83r4v3 14 41 endothelial endothelial JJ cord-308950-bl83r4v3 14 42 and and CC cord-308950-bl83r4v3 14 43 epithelial epithelial JJ cord-308950-bl83r4v3 14 44 cells cell NNS cord-308950-bl83r4v3 14 45 of of IN cord-308950-bl83r4v3 14 46 the the DT cord-308950-bl83r4v3 14 47 respiratory respiratory JJ cord-308950-bl83r4v3 14 48 tract tract NN cord-308950-bl83r4v3 14 49 [ [ -LRB- cord-308950-bl83r4v3 14 50 8 8 CD cord-308950-bl83r4v3 14 51 , , , cord-308950-bl83r4v3 14 52 12 12 CD cord-308950-bl83r4v3 14 53 , , , cord-308950-bl83r4v3 14 54 34 34 CD cord-308950-bl83r4v3 14 55 , , , cord-308950-bl83r4v3 14 56 35 35 CD cord-308950-bl83r4v3 14 57 ] ] -RRB- cord-308950-bl83r4v3 14 58 . . . cord-308950-bl83r4v3 15 1 Most most RBS cord-308950-bl83r4v3 15 2 recently recently RB cord-308950-bl83r4v3 15 3 APN APN NNP cord-308950-bl83r4v3 15 4 has have VBZ cord-308950-bl83r4v3 15 5 been be VBN cord-308950-bl83r4v3 15 6 isolated isolate VBN cord-308950-bl83r4v3 15 7 from from IN cord-308950-bl83r4v3 15 8 chicken chicken NN cord-308950-bl83r4v3 15 9 embryo embryo NN cord-308950-bl83r4v3 15 10 yolk yolk NN cord-308950-bl83r4v3 15 11 [ [ -LRB- cord-308950-bl83r4v3 15 12 32 32 CD cord-308950-bl83r4v3 15 13 ] ] -RRB- cord-308950-bl83r4v3 15 14 and and CC cord-308950-bl83r4v3 15 15 chicken chicken NN cord-308950-bl83r4v3 15 16 intestine intestine NN cord-308950-bl83r4v3 15 17 [ [ -LRB- cord-308950-bl83r4v3 15 18 14 14 CD cord-308950-bl83r4v3 15 19 ] ] -RRB- cord-308950-bl83r4v3 15 20 . . . cord-308950-bl83r4v3 16 1 The the DT cord-308950-bl83r4v3 16 2 feline feline JJ cord-308950-bl83r4v3 16 3 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 16 4 N N NNP cord-308950-bl83r4v3 16 5 has have VBZ cord-308950-bl83r4v3 16 6 been be VBN cord-308950-bl83r4v3 16 7 shown show VBN cord-308950-bl83r4v3 16 8 to to TO cord-308950-bl83r4v3 16 9 serve serve VB cord-308950-bl83r4v3 16 10 as as IN cord-308950-bl83r4v3 16 11 a a DT cord-308950-bl83r4v3 16 12 receptor receptor NN cord-308950-bl83r4v3 16 13 for for IN cord-308950-bl83r4v3 16 14 feline feline JJ cord-308950-bl83r4v3 16 15 , , , cord-308950-bl83r4v3 16 16 canine canine JJ cord-308950-bl83r4v3 16 17 , , , cord-308950-bl83r4v3 16 18 porcine porcine JJ cord-308950-bl83r4v3 16 19 and and CC cord-308950-bl83r4v3 16 20 human human JJ cord-308950-bl83r4v3 16 21 coronaviruses coronaviruse NNS cord-308950-bl83r4v3 16 22 in in IN cord-308950-bl83r4v3 16 23 group group NN cord-308950-bl83r4v3 17 1 I -PRON- PRP cord-308950-bl83r4v3 17 2 [ [ -LRB- cord-308950-bl83r4v3 17 3 38 38 CD cord-308950-bl83r4v3 17 4 , , , cord-308950-bl83r4v3 17 5 39 39 CD cord-308950-bl83r4v3 17 6 ] ] -RRB- cord-308950-bl83r4v3 17 7 . . . cord-308950-bl83r4v3 18 1 The the DT cord-308950-bl83r4v3 18 2 coronaviruses coronaviruse NNS cord-308950-bl83r4v3 18 3 from from IN cord-308950-bl83r4v3 18 4 group group NN cord-308950-bl83r4v3 18 5 I -PRON- PRP cord-308950-bl83r4v3 18 6 cause cause VBP cord-308950-bl83r4v3 18 7 disease disease NN cord-308950-bl83r4v3 18 8 only only RB cord-308950-bl83r4v3 18 9 in in IN cord-308950-bl83r4v3 18 10 their -PRON- PRP$ cord-308950-bl83r4v3 18 11 usual usual JJ cord-308950-bl83r4v3 18 12 target target NN cord-308950-bl83r4v3 18 13 host host NN cord-308950-bl83r4v3 18 14 species specie NNS cord-308950-bl83r4v3 18 15 . . . cord-308950-bl83r4v3 19 1 However however RB cord-308950-bl83r4v3 19 2 , , , cord-308950-bl83r4v3 19 3 it -PRON- PRP cord-308950-bl83r4v3 19 4 has have VBZ cord-308950-bl83r4v3 19 5 been be VBN cord-308950-bl83r4v3 19 6 reported report VBN cord-308950-bl83r4v3 19 7 that that IN cord-308950-bl83r4v3 19 8 some some DT cord-308950-bl83r4v3 19 9 strains strain NNS cord-308950-bl83r4v3 19 10 of of IN cord-308950-bl83r4v3 19 11 canine canine JJ cord-308950-bl83r4v3 19 12 coronavirus coronavirus NN cord-308950-bl83r4v3 19 13 ( ( -LRB- cord-308950-bl83r4v3 19 14 CCV CCV NNP cord-308950-bl83r4v3 19 15 ) ) -RRB- cord-308950-bl83r4v3 19 16 and and CC cord-308950-bl83r4v3 19 17 human human JJ cord-308950-bl83r4v3 19 18 coronavirus coronavirus NN cord-308950-bl83r4v3 19 19 229E 229e CD cord-308950-bl83r4v3 19 20 can can MD cord-308950-bl83r4v3 19 21 infect infect VB cord-308950-bl83r4v3 19 22 other other JJ cord-308950-bl83r4v3 19 23 non non JJ cord-308950-bl83r4v3 19 24 - - JJ cord-308950-bl83r4v3 19 25 target target JJ cord-308950-bl83r4v3 19 26 species specie NNS cord-308950-bl83r4v3 19 27 without without IN cord-308950-bl83r4v3 19 28 causing cause VBG cord-308950-bl83r4v3 19 29 disease disease NN cord-308950-bl83r4v3 19 30 [ [ -LRB- cord-308950-bl83r4v3 19 31 4 4 CD cord-308950-bl83r4v3 19 32 , , , cord-308950-bl83r4v3 19 33 5 5 CD cord-308950-bl83r4v3 19 34 ] ] -RRB- cord-308950-bl83r4v3 19 35 . . . cord-308950-bl83r4v3 20 1 Cats cat NNS cord-308950-bl83r4v3 20 2 inoculated inoculate VBD cord-308950-bl83r4v3 20 3 with with IN cord-308950-bl83r4v3 20 4 human human JJ cord-308950-bl83r4v3 20 5 coronavirus coronavirus NN cord-308950-bl83r4v3 20 6 229E 229e CD cord-308950-bl83r4v3 20 7 or or CC cord-308950-bl83r4v3 20 8 CCV CCV NNP cord-308950-bl83r4v3 20 9 seroconverted seroconverte VBD cord-308950-bl83r4v3 20 10 and and CC cord-308950-bl83r4v3 20 11 in in IN cord-308950-bl83r4v3 20 12 many many JJ cord-308950-bl83r4v3 20 13 cases case NNS cord-308950-bl83r4v3 20 14 shed shed VBP cord-308950-bl83r4v3 20 15 the the DT cord-308950-bl83r4v3 20 16 virus virus NN cord-308950-bl83r4v3 20 17 with with IN cord-308950-bl83r4v3 20 18 no no DT cord-308950-bl83r4v3 20 19 signs sign NNS cord-308950-bl83r4v3 20 20 of of IN cord-308950-bl83r4v3 20 21 clinical clinical JJ cord-308950-bl83r4v3 20 22 disease disease NN cord-308950-bl83r4v3 20 23 [ [ -LRB- cord-308950-bl83r4v3 20 24 4 4 CD cord-308950-bl83r4v3 20 25 , , , cord-308950-bl83r4v3 20 26 5 5 CD cord-308950-bl83r4v3 20 27 ] ] -RRB- cord-308950-bl83r4v3 20 28 . . . cord-308950-bl83r4v3 21 1 Infectious infectious JJ cord-308950-bl83r4v3 21 2 bronchitis bronchitis NN cord-308950-bl83r4v3 21 3 virus virus NN cord-308950-bl83r4v3 21 4 ( ( -LRB- cord-308950-bl83r4v3 21 5 IBV IBV NNP cord-308950-bl83r4v3 21 6 ) ) -RRB- cord-308950-bl83r4v3 21 7 is be VBZ cord-308950-bl83r4v3 21 8 a a DT cord-308950-bl83r4v3 21 9 member member NN cord-308950-bl83r4v3 21 10 of of IN cord-308950-bl83r4v3 21 11 the the DT cord-308950-bl83r4v3 21 12 group group NN cord-308950-bl83r4v3 21 13 III III NNP cord-308950-bl83r4v3 21 14 of of IN cord-308950-bl83r4v3 21 15 Coronaviridae Coronaviridae NNP cord-308950-bl83r4v3 21 16 . . . cord-308950-bl83r4v3 22 1 This this DT cord-308950-bl83r4v3 22 2 virus virus NN cord-308950-bl83r4v3 22 3 causes cause VBZ cord-308950-bl83r4v3 22 4 severe severe JJ cord-308950-bl83r4v3 22 5 respiratory respiratory JJ cord-308950-bl83r4v3 22 6 disease disease NN cord-308950-bl83r4v3 22 7 in in IN cord-308950-bl83r4v3 22 8 chickens chicken NNS cord-308950-bl83r4v3 22 9 , , , cord-308950-bl83r4v3 22 10 and and CC cord-308950-bl83r4v3 22 11 its -PRON- PRP$ cord-308950-bl83r4v3 22 12 receptor receptor NN cord-308950-bl83r4v3 22 13 has have VBZ cord-308950-bl83r4v3 22 14 not not RB cord-308950-bl83r4v3 22 15 been be VBN cord-308950-bl83r4v3 22 16 identified identify VBN cord-308950-bl83r4v3 22 17 . . . cord-308950-bl83r4v3 23 1 The the DT cord-308950-bl83r4v3 23 2 objectives objective NNS cord-308950-bl83r4v3 23 3 of of IN cord-308950-bl83r4v3 23 4 this this DT cord-308950-bl83r4v3 23 5 study study NN cord-308950-bl83r4v3 23 6 were be VBD cord-308950-bl83r4v3 23 7 to to IN cord-308950-bl83r4v3 23 8 : : : cord-308950-bl83r4v3 23 9 ( ( -LRB- cord-308950-bl83r4v3 23 10 1 1 LS cord-308950-bl83r4v3 23 11 ) ) -RRB- cord-308950-bl83r4v3 23 12 determine determine VB cord-308950-bl83r4v3 23 13 if if IN cord-308950-bl83r4v3 23 14 feline feline JJ cord-308950-bl83r4v3 23 15 cells cell NNS cord-308950-bl83r4v3 23 16 were be VBD cord-308950-bl83r4v3 23 17 permissive permissive JJ cord-308950-bl83r4v3 23 18 to to IN cord-308950-bl83r4v3 23 19 Arkansas Arkansas NNP cord-308950-bl83r4v3 23 20 99 99 CD cord-308950-bl83r4v3 24 1 ( ( -LRB- cord-308950-bl83r4v3 24 2 Ark Ark NNP cord-308950-bl83r4v3 24 3 99 99 CD cord-308950-bl83r4v3 24 4 ) ) -RRB- cord-308950-bl83r4v3 24 5 serotype serotype NN cord-308950-bl83r4v3 24 6 of of IN cord-308950-bl83r4v3 24 7 IBV IBV NNP cord-308950-bl83r4v3 24 8 , , , cord-308950-bl83r4v3 24 9 ( ( -LRB- cord-308950-bl83r4v3 24 10 2 2 LS cord-308950-bl83r4v3 24 11 ) ) -RRB- cord-308950-bl83r4v3 24 12 evaluate evaluate VB cord-308950-bl83r4v3 24 13 if if IN cord-308950-bl83r4v3 24 14 the the DT cord-308950-bl83r4v3 24 15 IBV IBV NNP cord-308950-bl83r4v3 24 16 can can MD cord-308950-bl83r4v3 24 17 replicate replicate VB cord-308950-bl83r4v3 24 18 in in IN cord-308950-bl83r4v3 24 19 feline feline JJ cord-308950-bl83r4v3 24 20 cells cell NNS cord-308950-bl83r4v3 24 21 , , , cord-308950-bl83r4v3 24 22 and and CC cord-308950-bl83r4v3 24 23 ( ( -LRB- cord-308950-bl83r4v3 24 24 3 3 LS cord-308950-bl83r4v3 24 25 ) ) -RRB- cord-308950-bl83r4v3 24 26 determine determine VB cord-308950-bl83r4v3 24 27 the the DT cord-308950-bl83r4v3 24 28 contribution contribution NN cord-308950-bl83r4v3 24 29 of of IN cord-308950-bl83r4v3 24 30 APN APN NNP cord-308950-bl83r4v3 24 31 to to IN cord-308950-bl83r4v3 24 32 permissiveness permissiveness NN cord-308950-bl83r4v3 24 33 of of IN cord-308950-bl83r4v3 24 34 feline feline JJ cord-308950-bl83r4v3 24 35 cells cell NNS cord-308950-bl83r4v3 24 36 . . . cord-308950-bl83r4v3 25 1 A a DT cord-308950-bl83r4v3 25 2 cell cell NN cord-308950-bl83r4v3 25 3 line line NN cord-308950-bl83r4v3 25 4 derived derive VBN cord-308950-bl83r4v3 25 5 from from IN cord-308950-bl83r4v3 25 6 a a DT cord-308950-bl83r4v3 25 7 normal normal JJ cord-308950-bl83r4v3 25 8 kidney kidney NN cord-308950-bl83r4v3 25 9 cortex cortex NN cord-308950-bl83r4v3 25 10 of of IN cord-308950-bl83r4v3 25 11 a a DT cord-308950-bl83r4v3 25 12 12-week 12-week JJ cord-308950-bl83r4v3 25 13 - - HYPH cord-308950-bl83r4v3 25 14 old old JJ cord-308950-bl83r4v3 25 15 female female JJ cord-308950-bl83r4v3 25 16 feline feline NN cord-308950-bl83r4v3 25 17 ( ( -LRB- cord-308950-bl83r4v3 25 18 Felis Felis NNP cord-308950-bl83r4v3 25 19 catus catus NN cord-308950-bl83r4v3 25 20 ) ) -RRB- cord-308950-bl83r4v3 25 21 ( ( -LRB- cord-308950-bl83r4v3 25 22 CRFK CRFK NNP cord-308950-bl83r4v3 25 23 - - HYPH cord-308950-bl83r4v3 25 24 CCL-94 CCL-94 NNP cord-308950-bl83r4v3 25 25 ) ) -RRB- cord-308950-bl83r4v3 25 26 was be VBD cord-308950-bl83r4v3 25 27 purchased purchase VBN cord-308950-bl83r4v3 25 28 from from IN cord-308950-bl83r4v3 25 29 American American NNP cord-308950-bl83r4v3 25 30 Type Type NNP cord-308950-bl83r4v3 25 31 Culture Culture NNP cord-308950-bl83r4v3 25 32 Collection Collection NNP cord-308950-bl83r4v3 25 33 ( ( -LRB- cord-308950-bl83r4v3 25 34 Manassas Manassas NNP cord-308950-bl83r4v3 25 35 , , , cord-308950-bl83r4v3 25 36 VA VA NNP cord-308950-bl83r4v3 25 37 ) ) -RRB- cord-308950-bl83r4v3 25 38 and and CC cord-308950-bl83r4v3 25 39 is be VBZ cord-308950-bl83r4v3 25 40 henceforth henceforth RB cord-308950-bl83r4v3 25 41 designated designate VBN cord-308950-bl83r4v3 25 42 as as IN cord-308950-bl83r4v3 25 43 FEK FEK NNP cord-308950-bl83r4v3 25 44 . . . cord-308950-bl83r4v3 26 1 The the DT cord-308950-bl83r4v3 26 2 cells cell NNS cord-308950-bl83r4v3 26 3 were be VBD cord-308950-bl83r4v3 26 4 cultured cultured JJ cord-308950-bl83r4v3 26 5 in in IN cord-308950-bl83r4v3 26 6 Dulbecco Dulbecco NNP cord-308950-bl83r4v3 26 7 's 's POS cord-308950-bl83r4v3 26 8 modified modify VBN cord-308950-bl83r4v3 26 9 eagle eagle NN cord-308950-bl83r4v3 26 10 medium medium NN cord-308950-bl83r4v3 26 11 ( ( -LRB- cord-308950-bl83r4v3 26 12 DMEM DMEM NNP cord-308950-bl83r4v3 26 13 ) ) -RRB- cord-308950-bl83r4v3 27 1 ( ( -LRB- cord-308950-bl83r4v3 27 2 Sigma Sigma NNP cord-308950-bl83r4v3 27 3 Chemical Chemical NNP cord-308950-bl83r4v3 27 4 Co. Co. NNP cord-308950-bl83r4v3 27 5 , , , cord-308950-bl83r4v3 27 6 St. St. NNP cord-308950-bl83r4v3 27 7 Louis Louis NNP cord-308950-bl83r4v3 27 8 , , , cord-308950-bl83r4v3 27 9 MO MO NNP cord-308950-bl83r4v3 27 10 ) ) -RRB- cord-308950-bl83r4v3 27 11 consisting consist VBG cord-308950-bl83r4v3 27 12 of of IN cord-308950-bl83r4v3 27 13 10 10 CD cord-308950-bl83r4v3 27 14 % % NN cord-308950-bl83r4v3 27 15 fetal fetal JJ cord-308950-bl83r4v3 27 16 bovine bovine JJ cord-308950-bl83r4v3 27 17 serum serum NN cord-308950-bl83r4v3 27 18 ( ( -LRB- cord-308950-bl83r4v3 27 19 FBS FBS NNP cord-308950-bl83r4v3 27 20 ) ) -RRB- cord-308950-bl83r4v3 27 21 , , , cord-308950-bl83r4v3 27 22 100 100 CD cord-308950-bl83r4v3 27 23 IU IU NNP cord-308950-bl83r4v3 27 24 / / , cord-308950-bl83r4v3 27 25 ml ml CC cord-308950-bl83r4v3 27 26 penicillin penicillin NN cord-308950-bl83r4v3 27 27 and and CC cord-308950-bl83r4v3 27 28 250 250 CD cord-308950-bl83r4v3 27 29 µg µg NN cord-308950-bl83r4v3 27 30 / / SYM cord-308950-bl83r4v3 27 31 ml ml NN cord-308950-bl83r4v3 28 1 streptomycin streptomycin NNP cord-308950-bl83r4v3 28 2 ( ( -LRB- cord-308950-bl83r4v3 28 3 Sigma Sigma NNP cord-308950-bl83r4v3 28 4 ) ) -RRB- cord-308950-bl83r4v3 28 5 . . . cord-308950-bl83r4v3 29 1 The the DT cord-308950-bl83r4v3 29 2 cells cell NNS cord-308950-bl83r4v3 29 3 were be VBD cord-308950-bl83r4v3 29 4 incubated incubate VBN cord-308950-bl83r4v3 29 5 in in IN cord-308950-bl83r4v3 29 6 5 5 CD cord-308950-bl83r4v3 29 7 % % NN cord-308950-bl83r4v3 29 8 CO co NN cord-308950-bl83r4v3 29 9 2 2 CD cord-308950-bl83r4v3 29 10 , , , cord-308950-bl83r4v3 29 11 90 90 CD cord-308950-bl83r4v3 29 12 % % NN cord-308950-bl83r4v3 29 13 humidity humidity NN cord-308950-bl83r4v3 29 14 at at IN cord-308950-bl83r4v3 29 15 37 37 CD cord-308950-bl83r4v3 29 16 • • NN cord-308950-bl83r4v3 29 17 C C NNP cord-308950-bl83r4v3 29 18 until until IN cord-308950-bl83r4v3 29 19 they -PRON- PRP cord-308950-bl83r4v3 29 20 were be VBD cord-308950-bl83r4v3 29 21 confluent confluent JJ cord-308950-bl83r4v3 29 22 . . . cord-308950-bl83r4v3 30 1 For for IN cord-308950-bl83r4v3 30 2 the the DT cord-308950-bl83r4v3 30 3 IFA IFA NNP cord-308950-bl83r4v3 30 4 assay assay NN cord-308950-bl83r4v3 30 5 , , , cord-308950-bl83r4v3 30 6 4 4 CD cord-308950-bl83r4v3 30 7 × × NN cord-308950-bl83r4v3 30 8 10 10 CD cord-308950-bl83r4v3 30 9 4 4 CD cord-308950-bl83r4v3 30 10 cells cell NNS cord-308950-bl83r4v3 30 11 / / SYM cord-308950-bl83r4v3 30 12 ml ml NNS cord-308950-bl83r4v3 30 13 were be VBD cord-308950-bl83r4v3 30 14 seeded seed VBN cord-308950-bl83r4v3 30 15 into into IN cord-308950-bl83r4v3 30 16 6-well 6-well CD cord-308950-bl83r4v3 30 17 tissue tissue NN cord-308950-bl83r4v3 30 18 culture culture NN cord-308950-bl83r4v3 30 19 plates plate NNS cord-308950-bl83r4v3 30 20 ( ( -LRB- cord-308950-bl83r4v3 30 21 Becton Becton NNP cord-308950-bl83r4v3 30 22 Dickinson Dickinson NNP cord-308950-bl83r4v3 30 23 Labware Labware NNP cord-308950-bl83r4v3 30 24 , , , cord-308950-bl83r4v3 30 25 Franklin Franklin NNP cord-308950-bl83r4v3 30 26 Lakes Lakes NNP cord-308950-bl83r4v3 30 27 , , , cord-308950-bl83r4v3 30 28 NJ NJ NNP cord-308950-bl83r4v3 30 29 ) ) -RRB- cord-308950-bl83r4v3 30 30 containing contain VBG cord-308950-bl83r4v3 30 31 18 18 CD cord-308950-bl83r4v3 30 32 × × NN cord-308950-bl83r4v3 30 33 18 18 CD cord-308950-bl83r4v3 30 34 mm mm NNP cord-308950-bl83r4v3 30 35 coverslips coverslip NNS cord-308950-bl83r4v3 30 36 . . . cord-308950-bl83r4v3 31 1 The the DT cord-308950-bl83r4v3 31 2 cells cell NNS cord-308950-bl83r4v3 31 3 were be VBD cord-308950-bl83r4v3 31 4 used use VBN cord-308950-bl83r4v3 31 5 once once IN cord-308950-bl83r4v3 31 6 attachment attachment NN cord-308950-bl83r4v3 31 7 had have VBD cord-308950-bl83r4v3 31 8 occurred occur VBN cord-308950-bl83r4v3 31 9 ( ( -LRB- cord-308950-bl83r4v3 31 10 24 24 CD cord-308950-bl83r4v3 31 11 h h NN cord-308950-bl83r4v3 31 12 ) ) -RRB- cord-308950-bl83r4v3 31 13 . . . cord-308950-bl83r4v3 32 1 A a DT cord-308950-bl83r4v3 32 2 cell cell NN cord-308950-bl83r4v3 32 3 line line NN cord-308950-bl83r4v3 32 4 of of IN cord-308950-bl83r4v3 32 5 normal normal JJ cord-308950-bl83r4v3 32 6 hamster hamster NN cord-308950-bl83r4v3 32 7 kidney kidney NN cord-308950-bl83r4v3 32 8 fibroblasts fibroblast NNS cord-308950-bl83r4v3 32 9 ( ( -LRB- cord-308950-bl83r4v3 32 10 BHK-21 BHK-21 NNP cord-308950-bl83r4v3 32 11 ) ) -RRB- cord-308950-bl83r4v3 32 12 was be VBD cord-308950-bl83r4v3 32 13 used use VBN cord-308950-bl83r4v3 32 14 as as IN cord-308950-bl83r4v3 32 15 control control NN cord-308950-bl83r4v3 32 16 cells cell NNS cord-308950-bl83r4v3 32 17 for for IN cord-308950-bl83r4v3 32 18 the the DT cord-308950-bl83r4v3 32 19 indirect indirect JJ cord-308950-bl83r4v3 32 20 immunofluorescence immunofluorescence NN cord-308950-bl83r4v3 32 21 assay assay NN cord-308950-bl83r4v3 32 22 ( ( -LRB- cord-308950-bl83r4v3 32 23 IFA IFA NNP cord-308950-bl83r4v3 32 24 ) ) -RRB- cord-308950-bl83r4v3 32 25 . . . cord-308950-bl83r4v3 33 1 The the DT cord-308950-bl83r4v3 33 2 medium medium NN cord-308950-bl83r4v3 33 3 and and CC cord-308950-bl83r4v3 33 4 culture culture NN cord-308950-bl83r4v3 33 5 conditions condition NNS cord-308950-bl83r4v3 33 6 for for IN cord-308950-bl83r4v3 33 7 BHK BHK NNP cord-308950-bl83r4v3 33 8 cells cell NNS cord-308950-bl83r4v3 33 9 were be VBD cord-308950-bl83r4v3 33 10 identical identical JJ cord-308950-bl83r4v3 33 11 to to IN cord-308950-bl83r4v3 33 12 those those DT cord-308950-bl83r4v3 33 13 used use VBN cord-308950-bl83r4v3 33 14 for for IN cord-308950-bl83r4v3 33 15 FEK FEK NNP cord-308950-bl83r4v3 33 16 cells cell NNS cord-308950-bl83r4v3 33 17 . . . cord-308950-bl83r4v3 34 1 A a DT cord-308950-bl83r4v3 34 2 field field NN cord-308950-bl83r4v3 34 3 strain strain NN cord-308950-bl83r4v3 34 4 of of IN cord-308950-bl83r4v3 34 5 Ark Ark NNP cord-308950-bl83r4v3 34 6 99 99 CD cord-308950-bl83r4v3 34 7 of of IN cord-308950-bl83r4v3 34 8 IBV IBV NNP cord-308950-bl83r4v3 34 9 provided provide VBN cord-308950-bl83r4v3 34 10 by by IN cord-308950-bl83r4v3 34 11 Dr. Dr. NNP cord-308950-bl83r4v3 34 12 J. J. NNP cord-308950-bl83r4v3 34 13 Gelb Gelb NNP cord-308950-bl83r4v3 34 14 Jr. Jr. NNP cord-308950-bl83r4v3 34 15 ( ( -LRB- cord-308950-bl83r4v3 34 16 Dept Dept NNP cord-308950-bl83r4v3 34 17 . . NNP cord-308950-bl83r4v3 34 18 of of IN cord-308950-bl83r4v3 34 19 Animal Animal NNP cord-308950-bl83r4v3 34 20 and and CC cord-308950-bl83r4v3 34 21 Food Food NNP cord-308950-bl83r4v3 34 22 Sciences Sciences NNPS cord-308950-bl83r4v3 34 23 , , , cord-308950-bl83r4v3 34 24 University University NNP cord-308950-bl83r4v3 34 25 of of IN cord-308950-bl83r4v3 34 26 Delaware Delaware NNP cord-308950-bl83r4v3 34 27 , , , cord-308950-bl83r4v3 34 28 Newark Newark NNP cord-308950-bl83r4v3 34 29 , , , cord-308950-bl83r4v3 34 30 DE DE NNP cord-308950-bl83r4v3 34 31 ) ) -RRB- cord-308950-bl83r4v3 34 32 was be VBD cord-308950-bl83r4v3 34 33 propagated propagate VBN cord-308950-bl83r4v3 34 34 by by IN cord-308950-bl83r4v3 34 35 passage passage NN cord-308950-bl83r4v3 34 36 in in IN cord-308950-bl83r4v3 34 37 specific specific JJ cord-308950-bl83r4v3 34 38 pathogen pathogen NN cord-308950-bl83r4v3 34 39 free free JJ cord-308950-bl83r4v3 34 40 ( ( -LRB- cord-308950-bl83r4v3 34 41 SPF SPF NNP cord-308950-bl83r4v3 34 42 ) ) -RRB- cord-308950-bl83r4v3 34 43 10-day 10-day CD cord-308950-bl83r4v3 34 44 - - HYPH cord-308950-bl83r4v3 34 45 old old JJ cord-308950-bl83r4v3 34 46 embryonated embryonate VBN cord-308950-bl83r4v3 34 47 chicken chicken NN cord-308950-bl83r4v3 34 48 eggs egg NNS cord-308950-bl83r4v3 34 49 ( ( -LRB- cord-308950-bl83r4v3 34 50 Hy Hy NNP cord-308950-bl83r4v3 34 51 - - HYPH cord-308950-bl83r4v3 34 52 Vac Vac NNP cord-308950-bl83r4v3 34 53 , , , cord-308950-bl83r4v3 34 54 Adel Adel NNP cord-308950-bl83r4v3 34 55 , , , cord-308950-bl83r4v3 34 56 Iowa Iowa NNP cord-308950-bl83r4v3 34 57 ) ) -RRB- cord-308950-bl83r4v3 34 58 . . . cord-308950-bl83r4v3 35 1 The the DT cord-308950-bl83r4v3 35 2 allantoic allantoic NNP cord-308950-bl83r4v3 35 3 fluid fluid NN cord-308950-bl83r4v3 35 4 was be VBD cord-308950-bl83r4v3 35 5 harvested harvest VBN cord-308950-bl83r4v3 35 6 24 24 CD cord-308950-bl83r4v3 35 7 h h NN cord-308950-bl83r4v3 35 8 after after IN cord-308950-bl83r4v3 35 9 infection infection NN cord-308950-bl83r4v3 35 10 , , , cord-308950-bl83r4v3 35 11 clarified clarify VBN cord-308950-bl83r4v3 35 12 by by IN cord-308950-bl83r4v3 35 13 centrifugation centrifugation NN cord-308950-bl83r4v3 35 14 at at IN cord-308950-bl83r4v3 35 15 low low JJ cord-308950-bl83r4v3 35 16 speed speed NN cord-308950-bl83r4v3 35 17 ( ( -LRB- cord-308950-bl83r4v3 35 18 1,075 1,075 CD cord-308950-bl83r4v3 35 19 × × NN cord-308950-bl83r4v3 35 20 g g NN cord-308950-bl83r4v3 35 21 for for IN cord-308950-bl83r4v3 35 22 15 15 CD cord-308950-bl83r4v3 35 23 min min NNP cord-308950-bl83r4v3 35 24 ) ) -RRB- cord-308950-bl83r4v3 35 25 , , , cord-308950-bl83r4v3 35 26 and and CC cord-308950-bl83r4v3 35 27 the the DT cord-308950-bl83r4v3 35 28 pellet pellet NN cord-308950-bl83r4v3 35 29 was be VBD cord-308950-bl83r4v3 35 30 discarded discard VBN cord-308950-bl83r4v3 35 31 . . . cord-308950-bl83r4v3 36 1 The the DT cord-308950-bl83r4v3 36 2 virus virus NN cord-308950-bl83r4v3 36 3 was be VBD cord-308950-bl83r4v3 36 4 collected collect VBN cord-308950-bl83r4v3 36 5 from from IN cord-308950-bl83r4v3 36 6 the the DT cord-308950-bl83r4v3 36 7 supernatant supernatant JJ cord-308950-bl83r4v3 36 8 and and CC cord-308950-bl83r4v3 36 9 concentrated concentrate VBN cord-308950-bl83r4v3 36 10 by by IN cord-308950-bl83r4v3 36 11 overnight overnight JJ cord-308950-bl83r4v3 36 12 high high JJ cord-308950-bl83r4v3 36 13 - - HYPH cord-308950-bl83r4v3 36 14 speed speed NN cord-308950-bl83r4v3 36 15 centrifugation centrifugation NN cord-308950-bl83r4v3 36 16 ( ( -LRB- cord-308950-bl83r4v3 36 17 10,000 10,000 CD cord-308950-bl83r4v3 36 18 × × NN cord-308950-bl83r4v3 36 19 g g NN cord-308950-bl83r4v3 36 20 ) ) -RRB- cord-308950-bl83r4v3 36 21 at at IN cord-308950-bl83r4v3 36 22 4 4 CD cord-308950-bl83r4v3 36 23 • • NN cord-308950-bl83r4v3 37 1 C. C. NNP cord-308950-bl83r4v3 37 2 The the DT cord-308950-bl83r4v3 37 3 supernatant supernatant NN cord-308950-bl83r4v3 37 4 was be VBD cord-308950-bl83r4v3 37 5 discarded discard VBN cord-308950-bl83r4v3 37 6 and and CC cord-308950-bl83r4v3 37 7 the the DT cord-308950-bl83r4v3 37 8 pellet pellet NN cord-308950-bl83r4v3 37 9 was be VBD cord-308950-bl83r4v3 37 10 resuspended resuspend VBN cord-308950-bl83r4v3 37 11 in in IN cord-308950-bl83r4v3 37 12 1 1 CD cord-308950-bl83r4v3 37 13 ml ml NNS cord-308950-bl83r4v3 37 14 of of IN cord-308950-bl83r4v3 37 15 TNE TNE NNP cord-308950-bl83r4v3 37 16 buffer buffer NN cord-308950-bl83r4v3 37 17 ( ( -LRB- cord-308950-bl83r4v3 37 18 10 10 CD cord-308950-bl83r4v3 37 19 mM mM NNP cord-308950-bl83r4v3 37 20 Tris Tris NNP cord-308950-bl83r4v3 37 21 - - HYPH cord-308950-bl83r4v3 37 22 HCl HCl NNP cord-308950-bl83r4v3 37 23 pH pH NNP cord-308950-bl83r4v3 37 24 7.4 7.4 CD cord-308950-bl83r4v3 37 25 , , , cord-308950-bl83r4v3 37 26 0.1 0.1 CD cord-308950-bl83r4v3 37 27 M m NN cord-308950-bl83r4v3 37 28 NaCl NaCl NNP cord-308950-bl83r4v3 37 29 , , , cord-308950-bl83r4v3 37 30 1 1 CD cord-308950-bl83r4v3 37 31 mM mm CD cord-308950-bl83r4v3 37 32 EDTA EDTA NNP cord-308950-bl83r4v3 37 33 ) ) -RRB- cord-308950-bl83r4v3 37 34 [ [ -LRB- cord-308950-bl83r4v3 37 35 16 16 CD cord-308950-bl83r4v3 37 36 ] ] -RRB- cord-308950-bl83r4v3 37 37 . . . cord-308950-bl83r4v3 38 1 The the DT cord-308950-bl83r4v3 38 2 virus virus NN cord-308950-bl83r4v3 38 3 suspension suspension NN cord-308950-bl83r4v3 38 4 was be VBD cord-308950-bl83r4v3 38 5 aliquoted aliquote VBN cord-308950-bl83r4v3 38 6 and and CC cord-308950-bl83r4v3 38 7 stored store VBN cord-308950-bl83r4v3 38 8 at at IN cord-308950-bl83r4v3 38 9 −70 −70 NNP cord-308950-bl83r4v3 38 10 • • NN cord-308950-bl83r4v3 38 11 C C NNP cord-308950-bl83r4v3 38 12 until until IN cord-308950-bl83r4v3 38 13 used use VBN cord-308950-bl83r4v3 38 14 . . . cord-308950-bl83r4v3 39 1 For for IN cord-308950-bl83r4v3 39 2 the the DT cord-308950-bl83r4v3 39 3 calculation calculation NN cord-308950-bl83r4v3 39 4 of of IN cord-308950-bl83r4v3 39 5 50 50 CD cord-308950-bl83r4v3 39 6 % % NN cord-308950-bl83r4v3 39 7 embryo embryo NN cord-308950-bl83r4v3 39 8 infectious infectious JJ cord-308950-bl83r4v3 39 9 dose dose NN cord-308950-bl83r4v3 39 10 ( ( -LRB- cord-308950-bl83r4v3 39 11 EID EID NNP cord-308950-bl83r4v3 39 12 50 50 CD cord-308950-bl83r4v3 39 13 ) ) -RRB- cord-308950-bl83r4v3 40 1 an an DT cord-308950-bl83r4v3 40 2 aliquot aliquot NN cord-308950-bl83r4v3 40 3 was be VBD cord-308950-bl83r4v3 40 4 titrated titrate VBN cord-308950-bl83r4v3 40 5 in in IN cord-308950-bl83r4v3 40 6 SPF SPF NNP cord-308950-bl83r4v3 40 7 embryonated embryonate VBN cord-308950-bl83r4v3 40 8 chicken chicken NN cord-308950-bl83r4v3 40 9 eggs egg NNS cord-308950-bl83r4v3 40 10 [ [ -LRB- cord-308950-bl83r4v3 40 11 40 40 CD cord-308950-bl83r4v3 40 12 ] ] -RRB- cord-308950-bl83r4v3 40 13 . . . cord-308950-bl83r4v3 41 1 The the DT cord-308950-bl83r4v3 41 2 feline feline JJ cord-308950-bl83r4v3 41 3 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 41 4 cDNA cdna NN cord-308950-bl83r4v3 41 5 ( ( -LRB- cord-308950-bl83r4v3 41 6 pBK pBK NNP cord-308950-bl83r4v3 41 7 - - HYPH cord-308950-bl83r4v3 41 8 fAPN fapn NN cord-308950-bl83r4v3 41 9 ) ) -RRB- cord-308950-bl83r4v3 41 10 subcloned subclone VBN cord-308950-bl83r4v3 41 11 into into IN cord-308950-bl83r4v3 41 12 the the DT cord-308950-bl83r4v3 41 13 plasmid plasmid NN cord-308950-bl83r4v3 41 14 pBK pbk NN cord-308950-bl83r4v3 41 15 - - HYPH cord-308950-bl83r4v3 41 16 CMV cmv NN cord-308950-bl83r4v3 41 17 vector vector NN cord-308950-bl83r4v3 41 18 ( ( -LRB- cord-308950-bl83r4v3 41 19 Stratagene Stratagene NNP cord-308950-bl83r4v3 41 20 ) ) -RRB- cord-308950-bl83r4v3 41 21 was be VBD cord-308950-bl83r4v3 41 22 kindly kindly RB cord-308950-bl83r4v3 41 23 provided provide VBN cord-308950-bl83r4v3 41 24 by by IN cord-308950-bl83r4v3 41 25 Dr. Dr. NNP cord-308950-bl83r4v3 41 26 Andreas Andreas NNP cord-308950-bl83r4v3 41 27 Kolb Kolb NNP cord-308950-bl83r4v3 41 28 , , , cord-308950-bl83r4v3 41 29 Hannah Hannah NNP cord-308950-bl83r4v3 41 30 Research Research NNP cord-308950-bl83r4v3 41 31 Institute Institute NNP cord-308950-bl83r4v3 41 32 , , , cord-308950-bl83r4v3 41 33 Ayr Ayr NNP cord-308950-bl83r4v3 41 34 , , , cord-308950-bl83r4v3 41 35 Scotland Scotland NNP cord-308950-bl83r4v3 41 36 , , , cord-308950-bl83r4v3 41 37 UK UK NNP cord-308950-bl83r4v3 41 38 . . . cord-308950-bl83r4v3 42 1 BHK-21 BHK-21 NNP cord-308950-bl83r4v3 43 1 cells cell NNS cord-308950-bl83r4v3 43 2 were be VBD cord-308950-bl83r4v3 43 3 transfected transfecte VBN cord-308950-bl83r4v3 43 4 with with IN cord-308950-bl83r4v3 43 5 pBK pBK NNP cord-308950-bl83r4v3 43 6 - - HYPH cord-308950-bl83r4v3 43 7 fAPN fapn NN cord-308950-bl83r4v3 43 8 or or CC cord-308950-bl83r4v3 43 9 pBK pbk NN cord-308950-bl83r4v3 43 10 - - HYPH cord-308950-bl83r4v3 43 11 CMV CMV NNP cord-308950-bl83r4v3 43 12 ( ( -LRB- cord-308950-bl83r4v3 43 13 control control NN cord-308950-bl83r4v3 43 14 plasmid plasmid NN cord-308950-bl83r4v3 43 15 ) ) -RRB- cord-308950-bl83r4v3 43 16 using use VBG cord-308950-bl83r4v3 43 17 LipofectAMINE LipofectAMINE NNP cord-308950-bl83r4v3 43 18 reagent reagent NN cord-308950-bl83r4v3 43 19 ( ( -LRB- cord-308950-bl83r4v3 43 20 Stratagene Stratagene NNP cord-308950-bl83r4v3 43 21 ) ) -RRB- cord-308950-bl83r4v3 43 22 . . . cord-308950-bl83r4v3 44 1 Transfected transfected JJ cord-308950-bl83r4v3 44 2 cells cell NNS cord-308950-bl83r4v3 44 3 were be VBD cord-308950-bl83r4v3 44 4 seeded seed VBN cord-308950-bl83r4v3 44 5 in in IN cord-308950-bl83r4v3 44 6 25-cm 25-cm CD cord-308950-bl83r4v3 44 7 2 2 CD cord-308950-bl83r4v3 44 8 flasks flask NNS cord-308950-bl83r4v3 44 9 for for IN cord-308950-bl83r4v3 44 10 24 24 CD cord-308950-bl83r4v3 44 11 h h NN cord-308950-bl83r4v3 44 12 , , , cord-308950-bl83r4v3 44 13 at at IN cord-308950-bl83r4v3 44 14 which which WDT cord-308950-bl83r4v3 44 15 time time NN cord-308950-bl83r4v3 44 16 the the DT cord-308950-bl83r4v3 44 17 medium medium NN cord-308950-bl83r4v3 44 18 was be VBD cord-308950-bl83r4v3 44 19 changed change VBN cord-308950-bl83r4v3 44 20 . . . cord-308950-bl83r4v3 45 1 The the DT cord-308950-bl83r4v3 45 2 cells cell NNS cord-308950-bl83r4v3 45 3 incubated incubate VBN cord-308950-bl83r4v3 45 4 for for IN cord-308950-bl83r4v3 45 5 an an DT cord-308950-bl83r4v3 45 6 additional additional JJ cord-308950-bl83r4v3 45 7 48 48 CD cord-308950-bl83r4v3 45 8 h h NN cord-308950-bl83r4v3 45 9 before before IN cord-308950-bl83r4v3 45 10 evaluation evaluation NN cord-308950-bl83r4v3 45 11 by by IN cord-308950-bl83r4v3 45 12 flow flow NN cord-308950-bl83r4v3 45 13 cytometry cytometry NN cord-308950-bl83r4v3 45 14 . . . cord-308950-bl83r4v3 46 1 To to TO cord-308950-bl83r4v3 46 2 evaluate evaluate VB cord-308950-bl83r4v3 46 3 the the DT cord-308950-bl83r4v3 46 4 permissiveness permissiveness NN cord-308950-bl83r4v3 46 5 of of IN cord-308950-bl83r4v3 46 6 cells cell NNS cord-308950-bl83r4v3 46 7 to to IN cord-308950-bl83r4v3 46 8 IBV IBV NNP cord-308950-bl83r4v3 46 9 , , , cord-308950-bl83r4v3 46 10 monolayers monolayer NNS cord-308950-bl83r4v3 46 11 of of IN cord-308950-bl83r4v3 46 12 transfected transfecte VBN cord-308950-bl83r4v3 46 13 ( ( -LRB- cord-308950-bl83r4v3 46 14 pBK pBK NNP cord-308950-bl83r4v3 46 15 - - HYPH cord-308950-bl83r4v3 46 16 fAPN fapn NN cord-308950-bl83r4v3 46 17 and and CC cord-308950-bl83r4v3 46 18 pBK pbk NN cord-308950-bl83r4v3 46 19 - - HYPH cord-308950-bl83r4v3 46 20 CMV CMV NNP cord-308950-bl83r4v3 46 21 ) ) -RRB- cord-308950-bl83r4v3 46 22 BHK-21 BHK-21 NNP cord-308950-bl83r4v3 46 23 , , , cord-308950-bl83r4v3 46 24 non non JJ cord-308950-bl83r4v3 46 25 - - JJ cord-308950-bl83r4v3 46 26 transfected transfected JJ cord-308950-bl83r4v3 46 27 BHK-21 BHK-21 NNP cord-308950-bl83r4v3 46 28 and and CC cord-308950-bl83r4v3 46 29 FEK FEK NNP cord-308950-bl83r4v3 46 30 on on IN cord-308950-bl83r4v3 46 31 coverslips coverslip NNS cord-308950-bl83r4v3 46 32 were be VBD cord-308950-bl83r4v3 46 33 infected infect VBN cord-308950-bl83r4v3 46 34 with with IN cord-308950-bl83r4v3 46 35 a a DT cord-308950-bl83r4v3 46 36 10 10 CD cord-308950-bl83r4v3 46 37 3.2 3.2 CD cord-308950-bl83r4v3 46 38 EID EID NNP cord-308950-bl83r4v3 46 39 50 50 CD cord-308950-bl83r4v3 46 40 of of IN cord-308950-bl83r4v3 46 41 Ark Ark NNP cord-308950-bl83r4v3 46 42 / / SYM cord-308950-bl83r4v3 46 43 IBV IBV NNP cord-308950-bl83r4v3 46 44 virus virus NN cord-308950-bl83r4v3 46 45 . . . cord-308950-bl83r4v3 47 1 The the DT cord-308950-bl83r4v3 47 2 cells cell NNS cord-308950-bl83r4v3 47 3 were be VBD cord-308950-bl83r4v3 47 4 washed wash VBN cord-308950-bl83r4v3 47 5 , , , cord-308950-bl83r4v3 47 6 fixed fix VBN cord-308950-bl83r4v3 47 7 in in IN cord-308950-bl83r4v3 47 8 ethanol ethanol NN cord-308950-bl83r4v3 47 9 , , , cord-308950-bl83r4v3 47 10 rinsed rinse VBN cord-308950-bl83r4v3 47 11 again again RB cord-308950-bl83r4v3 47 12 with with IN cord-308950-bl83r4v3 47 13 PBS PBS NNP cord-308950-bl83r4v3 47 14 ( ( -LRB- cord-308950-bl83r4v3 47 15 pH pH NNP cord-308950-bl83r4v3 47 16 7.4 7.4 CD cord-308950-bl83r4v3 47 17 ) ) -RRB- cord-308950-bl83r4v3 47 18 and and CC cord-308950-bl83r4v3 47 19 incubated incubate VBN cord-308950-bl83r4v3 47 20 with with IN cord-308950-bl83r4v3 47 21 a a DT cord-308950-bl83r4v3 47 22 monoclonal monoclonal JJ cord-308950-bl83r4v3 47 23 antibody antibody NN cord-308950-bl83r4v3 47 24 to to IN cord-308950-bl83r4v3 47 25 the the DT cord-308950-bl83r4v3 47 26 S1 S1 NNP cord-308950-bl83r4v3 47 27 glycoprotein glycoprotein NN cord-308950-bl83r4v3 47 28 of of IN cord-308950-bl83r4v3 47 29 Ark Ark NNP cord-308950-bl83r4v3 47 30 serotype serotype NN cord-308950-bl83r4v3 47 31 IBV IBV NNP cord-308950-bl83r4v3 47 32 monoclonal monoclonal JJ cord-308950-bl83r4v3 47 33 ( ( -LRB- cord-308950-bl83r4v3 47 34 Dr. Dr. NNP cord-308950-bl83r4v3 47 35 S. S. NNP cord-308950-bl83r4v3 47 36 A. A. NNP cord-308950-bl83r4v3 47 37 Naqi Naqi NNP cord-308950-bl83r4v3 47 38 , , , cord-308950-bl83r4v3 47 39 Dept Dept NNP cord-308950-bl83r4v3 47 40 . . NNP cord-308950-bl83r4v3 47 41 of of IN cord-308950-bl83r4v3 47 42 Microbiology Microbiology NNP cord-308950-bl83r4v3 47 43 and and CC cord-308950-bl83r4v3 47 44 Immunology Immunology NNP cord-308950-bl83r4v3 47 45 , , , cord-308950-bl83r4v3 47 46 Cornell Cornell NNP cord-308950-bl83r4v3 47 47 University University NNP cord-308950-bl83r4v3 47 48 , , , cord-308950-bl83r4v3 47 49 Ithaca Ithaca NNP cord-308950-bl83r4v3 47 50 , , , cord-308950-bl83r4v3 47 51 NY NY NNP cord-308950-bl83r4v3 47 52 ) ) -RRB- cord-308950-bl83r4v3 47 53 at at IN cord-308950-bl83r4v3 47 54 a a DT cord-308950-bl83r4v3 47 55 1:50 1:50 CD cord-308950-bl83r4v3 47 56 dilution dilution NN cord-308950-bl83r4v3 47 57 for for IN cord-308950-bl83r4v3 47 58 45 45 CD cord-308950-bl83r4v3 47 59 min min NN cord-308950-bl83r4v3 47 60 at at IN cord-308950-bl83r4v3 47 61 37 37 CD cord-308950-bl83r4v3 47 62 • • NN cord-308950-bl83r4v3 47 63 C. c. NN cord-308950-bl83r4v3 48 1 The the DT cord-308950-bl83r4v3 48 2 coverslips coverslip NNS cord-308950-bl83r4v3 48 3 were be VBD cord-308950-bl83r4v3 48 4 washed wash VBN cord-308950-bl83r4v3 48 5 in in IN cord-308950-bl83r4v3 48 6 PBS PBS NNP cord-308950-bl83r4v3 48 7 three three CD cord-308950-bl83r4v3 48 8 times time NNS cord-308950-bl83r4v3 48 9 for for IN cord-308950-bl83r4v3 48 10 5 5 CD cord-308950-bl83r4v3 48 11 min min NN cord-308950-bl83r4v3 48 12 each each DT cord-308950-bl83r4v3 48 13 and and CC cord-308950-bl83r4v3 48 14 then then RB cord-308950-bl83r4v3 48 15 incubated incubate VBD cord-308950-bl83r4v3 48 16 with with IN cord-308950-bl83r4v3 48 17 a a DT cord-308950-bl83r4v3 48 18 goat goat NN cord-308950-bl83r4v3 48 19 anti anti JJ cord-308950-bl83r4v3 48 20 - - JJ cord-308950-bl83r4v3 48 21 mouse mouse JJ cord-308950-bl83r4v3 48 22 IgG igg NN cord-308950-bl83r4v3 48 23 conjugated conjugate VBN cord-308950-bl83r4v3 48 24 to to IN cord-308950-bl83r4v3 48 25 fluorescein fluorescein NN cord-308950-bl83r4v3 48 26 isothiocyanate isothiocyanate NN cord-308950-bl83r4v3 48 27 ( ( -LRB- cord-308950-bl83r4v3 48 28 FITC FITC NNP cord-308950-bl83r4v3 48 29 ) ) -RRB- cord-308950-bl83r4v3 48 30 ( ( -LRB- cord-308950-bl83r4v3 48 31 Southern Southern NNP cord-308950-bl83r4v3 48 32 Biotechnologies Biotechnologies NNPS cord-308950-bl83r4v3 48 33 Associates Associates NNPS cord-308950-bl83r4v3 48 34 Inc. Inc. NNP cord-308950-bl83r4v3 48 35 , , , cord-308950-bl83r4v3 48 36 Birmingham Birmingham NNP cord-308950-bl83r4v3 48 37 , , , cord-308950-bl83r4v3 48 38 AL AL NNP cord-308950-bl83r4v3 48 39 ) ) -RRB- cord-308950-bl83r4v3 48 40 at at IN cord-308950-bl83r4v3 48 41 a a DT cord-308950-bl83r4v3 48 42 dilution dilution NN cord-308950-bl83r4v3 48 43 of of IN cord-308950-bl83r4v3 48 44 1:50 1:50 CD cord-308950-bl83r4v3 48 45 for for IN cord-308950-bl83r4v3 48 46 45 45 CD cord-308950-bl83r4v3 48 47 min min NN cord-308950-bl83r4v3 48 48 at at IN cord-308950-bl83r4v3 48 49 37 37 CD cord-308950-bl83r4v3 48 50 • • NN cord-308950-bl83r4v3 48 51 C. c. NN cord-308950-bl83r4v3 49 1 The the DT cord-308950-bl83r4v3 49 2 coverslips coverslip NNS cord-308950-bl83r4v3 49 3 were be VBD cord-308950-bl83r4v3 49 4 washed wash VBN cord-308950-bl83r4v3 49 5 three three CD cord-308950-bl83r4v3 49 6 times time NNS cord-308950-bl83r4v3 49 7 for for IN cord-308950-bl83r4v3 49 8 5 5 CD cord-308950-bl83r4v3 49 9 min min NN cord-308950-bl83r4v3 49 10 in in IN cord-308950-bl83r4v3 49 11 PBS PBS NNP cord-308950-bl83r4v3 49 12 , , , cord-308950-bl83r4v3 49 13 then then RB cord-308950-bl83r4v3 49 14 mounted mount VBD cord-308950-bl83r4v3 49 15 on on IN cord-308950-bl83r4v3 49 16 microscope microscope NN cord-308950-bl83r4v3 49 17 slides slide NNS cord-308950-bl83r4v3 49 18 with with IN cord-308950-bl83r4v3 49 19 mounting mount VBG cord-308950-bl83r4v3 49 20 medium medium NN cord-308950-bl83r4v3 49 21 ( ( -LRB- cord-308950-bl83r4v3 49 22 Dako Dako NNP cord-308950-bl83r4v3 49 23 Corporation Corporation NNP cord-308950-bl83r4v3 49 24 , , , cord-308950-bl83r4v3 49 25 Carpinteria Carpinteria NNP cord-308950-bl83r4v3 49 26 , , , cord-308950-bl83r4v3 49 27 CA CA NNP cord-308950-bl83r4v3 49 28 ) ) -RRB- cord-308950-bl83r4v3 49 29 . . . cord-308950-bl83r4v3 50 1 Slides slide NNS cord-308950-bl83r4v3 50 2 were be VBD cord-308950-bl83r4v3 50 3 viewed view VBN cord-308950-bl83r4v3 50 4 using use VBG cord-308950-bl83r4v3 50 5 epifluorescent epifluorescent NN cord-308950-bl83r4v3 50 6 microscopy microscopy NNS cord-308950-bl83r4v3 50 7 ( ( -LRB- cord-308950-bl83r4v3 50 8 Olympus Olympus NNP cord-308950-bl83r4v3 50 9 America America NNP cord-308950-bl83r4v3 50 10 , , , cord-308950-bl83r4v3 50 11 Inc. Inc. NNP cord-308950-bl83r4v3 50 12 , , , cord-308950-bl83r4v3 50 13 Lake Lake NNP cord-308950-bl83r4v3 50 14 Success Success NNP cord-308950-bl83r4v3 50 15 , , , cord-308950-bl83r4v3 50 16 N.Y. New York NNP cord-308950-bl83r4v3 50 17 ) ) -RRB- cord-308950-bl83r4v3 50 18 , , , cord-308950-bl83r4v3 50 19 and and CC cord-308950-bl83r4v3 50 20 confocal confocal JJ cord-308950-bl83r4v3 50 21 microscopy microscopy NNS cord-308950-bl83r4v3 50 22 ( ( -LRB- cord-308950-bl83r4v3 50 23 Leica Leica NNP cord-308950-bl83r4v3 50 24 , , , cord-308950-bl83r4v3 50 25 Heidelberg Heidelberg NNP cord-308950-bl83r4v3 50 26 , , , cord-308950-bl83r4v3 50 27 Germany Germany NNP cord-308950-bl83r4v3 50 28 ) ) -RRB- cord-308950-bl83r4v3 50 29 . . . cord-308950-bl83r4v3 51 1 FEK FEK NNP cord-308950-bl83r4v3 51 2 grown grow VBN cord-308950-bl83r4v3 51 3 on on IN cord-308950-bl83r4v3 51 4 6-well 6-well CD cord-308950-bl83r4v3 51 5 tissue tissue NN cord-308950-bl83r4v3 51 6 culture culture NN cord-308950-bl83r4v3 51 7 plates plate NNS cord-308950-bl83r4v3 51 8 were be VBD cord-308950-bl83r4v3 51 9 infected infect VBN cord-308950-bl83r4v3 51 10 with with IN cord-308950-bl83r4v3 51 11 a a DT cord-308950-bl83r4v3 51 12 10 10 CD cord-308950-bl83r4v3 51 13 3.2 3.2 CD cord-308950-bl83r4v3 51 14 EID EID NNP cord-308950-bl83r4v3 51 15 50 50 CD cord-308950-bl83r4v3 51 16 of of IN cord-308950-bl83r4v3 51 17 Ark Ark NNP cord-308950-bl83r4v3 51 18 / / SYM cord-308950-bl83r4v3 51 19 IBV IBV NNP cord-308950-bl83r4v3 51 20 virus virus NN cord-308950-bl83r4v3 51 21 . . . cord-308950-bl83r4v3 52 1 The the DT cord-308950-bl83r4v3 52 2 supernatant supernatant NN cord-308950-bl83r4v3 52 3 of of IN cord-308950-bl83r4v3 52 4 these these DT cord-308950-bl83r4v3 52 5 infected infect VBN cord-308950-bl83r4v3 52 6 cell cell NN cord-308950-bl83r4v3 52 7 cultures culture NNS cord-308950-bl83r4v3 52 8 was be VBD cord-308950-bl83r4v3 52 9 collected collect VBN cord-308950-bl83r4v3 52 10 at at IN cord-308950-bl83r4v3 52 11 1 1 CD cord-308950-bl83r4v3 52 12 , , , cord-308950-bl83r4v3 52 13 2 2 CD cord-308950-bl83r4v3 52 14 , , , cord-308950-bl83r4v3 52 15 3 3 CD cord-308950-bl83r4v3 52 16 and and CC cord-308950-bl83r4v3 52 17 10 10 CD cord-308950-bl83r4v3 52 18 d d NN cord-308950-bl83r4v3 52 19 post post NN cord-308950-bl83r4v3 52 20 infection infection NN cord-308950-bl83r4v3 52 21 . . . cord-308950-bl83r4v3 53 1 The the DT cord-308950-bl83r4v3 53 2 media medium NNS cord-308950-bl83r4v3 53 3 in in IN cord-308950-bl83r4v3 53 4 this this DT cord-308950-bl83r4v3 53 5 last last JJ cord-308950-bl83r4v3 53 6 culture culture NN cord-308950-bl83r4v3 53 7 was be VBD cord-308950-bl83r4v3 53 8 collected collect VBN cord-308950-bl83r4v3 53 9 twice twice RB cord-308950-bl83r4v3 53 10 , , , cord-308950-bl83r4v3 53 11 pooled pool VBN cord-308950-bl83r4v3 53 12 and and CC cord-308950-bl83r4v3 53 13 frozen freeze VBN cord-308950-bl83r4v3 53 14 at at IN cord-308950-bl83r4v3 53 15 −80 −80 NNP cord-308950-bl83r4v3 54 1 • • NNP cord-308950-bl83r4v3 54 2 C C NNP cord-308950-bl83r4v3 54 3 until until IN cord-308950-bl83r4v3 54 4 titrated titrate VBN cord-308950-bl83r4v3 54 5 . . . cord-308950-bl83r4v3 55 1 Virus virus NN cord-308950-bl83r4v3 55 2 titer titer NN cord-308950-bl83r4v3 55 3 was be VBD cord-308950-bl83r4v3 55 4 calculated calculate VBN cord-308950-bl83r4v3 55 5 using use VBG cord-308950-bl83r4v3 55 6 the the DT cord-308950-bl83r4v3 55 7 method method NN cord-308950-bl83r4v3 55 8 of of IN cord-308950-bl83r4v3 55 9 Reed Reed NNP cord-308950-bl83r4v3 55 10 and and CC cord-308950-bl83r4v3 55 11 Munch Munch NNP cord-308950-bl83r4v3 55 12 described describe VBD cord-308950-bl83r4v3 55 13 in in IN cord-308950-bl83r4v3 55 14 [ [ -LRB- cord-308950-bl83r4v3 55 15 40 40 CD cord-308950-bl83r4v3 55 16 ] ] -RRB- cord-308950-bl83r4v3 55 17 . . . cord-308950-bl83r4v3 56 1 Expression expression NN cord-308950-bl83r4v3 56 2 of of IN cord-308950-bl83r4v3 56 3 fAPN fapn NN cord-308950-bl83r4v3 56 4 on on IN cord-308950-bl83r4v3 56 5 the the DT cord-308950-bl83r4v3 56 6 surface surface NN cord-308950-bl83r4v3 56 7 of of IN cord-308950-bl83r4v3 56 8 transfected transfecte VBN cord-308950-bl83r4v3 56 9 BHK-21 BHK-21 NNP cord-308950-bl83r4v3 56 10 cells cell NNS cord-308950-bl83r4v3 56 11 was be VBD cord-308950-bl83r4v3 56 12 determined determine VBN cord-308950-bl83r4v3 56 13 using use VBG cord-308950-bl83r4v3 56 14 a a DT cord-308950-bl83r4v3 56 15 monoclonal monoclonal JJ cord-308950-bl83r4v3 56 16 antibody antibody NN cord-308950-bl83r4v3 56 17 to to IN cord-308950-bl83r4v3 56 18 human human JJ cord-308950-bl83r4v3 56 19 CD13 CD13 NNP cord-308950-bl83r4v3 56 20 clone clone NN cord-308950-bl83r4v3 57 1 WM-47 WM-47 NNP cord-308950-bl83r4v3 57 2 ( ( -LRB- cord-308950-bl83r4v3 57 3 Accurate Accurate NNP cord-308950-bl83r4v3 57 4 Antibodies Antibodies NNPS cord-308950-bl83r4v3 57 5 , , , cord-308950-bl83r4v3 57 6 Westbury Westbury NNP cord-308950-bl83r4v3 57 7 , , , cord-308950-bl83r4v3 57 8 NY NY NNP cord-308950-bl83r4v3 57 9 ) ) -RRB- cord-308950-bl83r4v3 57 10 that that IN cord-308950-bl83r4v3 57 11 cross cross NN cord-308950-bl83r4v3 57 12 - - VBZ cord-308950-bl83r4v3 57 13 reacts react VBZ cord-308950-bl83r4v3 57 14 with with IN cord-308950-bl83r4v3 57 15 the the DT cord-308950-bl83r4v3 57 16 feline feline JJ cord-308950-bl83r4v3 57 17 APN APN NNP cord-308950-bl83r4v3 57 18 ( ( -LRB- cord-308950-bl83r4v3 57 19 personal personal JJ cord-308950-bl83r4v3 57 20 communication communication NN cord-308950-bl83r4v3 57 21 with with IN cord-308950-bl83r4v3 57 22 Dr. Dr. NNP cord-308950-bl83r4v3 57 23 K. K. NNP cord-308950-bl83r4v3 57 24 V. V. NNP cord-308950-bl83r4v3 57 25 Holmes Holmes NNP cord-308950-bl83r4v3 57 26 ) ) -RRB- cord-308950-bl83r4v3 57 27 . . . cord-308950-bl83r4v3 58 1 BHK-21 BHK-21 NNP cord-308950-bl83r4v3 59 1 cells cell NNS cord-308950-bl83r4v3 59 2 were be VBD cord-308950-bl83r4v3 59 3 washed wash VBN cord-308950-bl83r4v3 59 4 two two CD cord-308950-bl83r4v3 59 5 times time NNS cord-308950-bl83r4v3 59 6 with with IN cord-308950-bl83r4v3 59 7 phosphate phosphate NN cord-308950-bl83r4v3 59 8 buffered buffer VBN cord-308950-bl83r4v3 59 9 saline saline NN cord-308950-bl83r4v3 59 10 ( ( -LRB- cord-308950-bl83r4v3 59 11 PBS PBS NNP cord-308950-bl83r4v3 59 12 ) ) -RRB- cord-308950-bl83r4v3 59 13 , , , cord-308950-bl83r4v3 59 14 incubated incubate VBN cord-308950-bl83r4v3 59 15 with with IN cord-308950-bl83r4v3 59 16 anti anti JJ cord-308950-bl83r4v3 59 17 - - JJ cord-308950-bl83r4v3 59 18 human human JJ cord-308950-bl83r4v3 59 19 CD13 CD13 NNP cord-308950-bl83r4v3 59 20 or or CC cord-308950-bl83r4v3 59 21 an an DT cord-308950-bl83r4v3 59 22 isotype isotype NN cord-308950-bl83r4v3 59 23 matched match VBN cord-308950-bl83r4v3 59 24 control control NN cord-308950-bl83r4v3 59 25 , , , cord-308950-bl83r4v3 59 26 washed wash VBN cord-308950-bl83r4v3 59 27 , , , cord-308950-bl83r4v3 59 28 and and CC cord-308950-bl83r4v3 59 29 then then RB cord-308950-bl83r4v3 59 30 incubated incubate VBD cord-308950-bl83r4v3 59 31 with with IN cord-308950-bl83r4v3 59 32 FITC FITC NNP cord-308950-bl83r4v3 59 33 conjugated conjugate VBN cord-308950-bl83r4v3 59 34 goat goat NN cord-308950-bl83r4v3 59 35 anti anti JJ cord-308950-bl83r4v3 59 36 - - NN cord-308950-bl83r4v3 59 37 mouse mouse NN cord-308950-bl83r4v3 60 1 IgG. IgG. NNP cord-308950-bl83r4v3 61 1 An an DT cord-308950-bl83r4v3 61 2 additional additional JJ cord-308950-bl83r4v3 61 3 control control NN cord-308950-bl83r4v3 61 4 included include VBD cord-308950-bl83r4v3 61 5 cells cell NNS cord-308950-bl83r4v3 61 6 with with IN cord-308950-bl83r4v3 61 7 the the DT cord-308950-bl83r4v3 61 8 secondary secondary JJ cord-308950-bl83r4v3 61 9 antibody antibody NN cord-308950-bl83r4v3 61 10 only only RB cord-308950-bl83r4v3 61 11 . . . cord-308950-bl83r4v3 62 1 All all DT cord-308950-bl83r4v3 62 2 incubations incubation NNS cord-308950-bl83r4v3 62 3 were be VBD cord-308950-bl83r4v3 62 4 done do VBN cord-308950-bl83r4v3 62 5 for for IN cord-308950-bl83r4v3 62 6 5 5 CD cord-308950-bl83r4v3 62 7 min min NN cord-308950-bl83r4v3 62 8 on on IN cord-308950-bl83r4v3 62 9 ice ice NN cord-308950-bl83r4v3 62 10 . . . cord-308950-bl83r4v3 63 1 After after IN cord-308950-bl83r4v3 63 2 the the DT cord-308950-bl83r4v3 63 3 final final JJ cord-308950-bl83r4v3 63 4 wash wash NN cord-308950-bl83r4v3 63 5 , , , cord-308950-bl83r4v3 63 6 all all DT cord-308950-bl83r4v3 63 7 cells cell NNS cord-308950-bl83r4v3 63 8 were be VBD cord-308950-bl83r4v3 63 9 suspended suspend VBN cord-308950-bl83r4v3 63 10 in in IN cord-308950-bl83r4v3 63 11 500 500 CD cord-308950-bl83r4v3 63 12 µl µl IN cord-308950-bl83r4v3 63 13 of of IN cord-308950-bl83r4v3 63 14 PBS PBS NNP cord-308950-bl83r4v3 63 15 and and CC cord-308950-bl83r4v3 63 16 analyzed analyze VBN cord-308950-bl83r4v3 63 17 by by IN cord-308950-bl83r4v3 63 18 flow flow NN cord-308950-bl83r4v3 63 19 cytometry cytometry NN cord-308950-bl83r4v3 63 20 ( ( -LRB- cord-308950-bl83r4v3 63 21 Calibur Calibur NNP cord-308950-bl83r4v3 63 22 Flow Flow NNP cord-308950-bl83r4v3 63 23 Cytometry Cytometry NNP cord-308950-bl83r4v3 63 24 System System NNP cord-308950-bl83r4v3 63 25 , , , cord-308950-bl83r4v3 63 26 San San NNP cord-308950-bl83r4v3 63 27 Jose Jose NNP cord-308950-bl83r4v3 63 28 , , , cord-308950-bl83r4v3 63 29 CA CA NNP cord-308950-bl83r4v3 63 30 ) ) -RRB- cord-308950-bl83r4v3 63 31 . . . cord-308950-bl83r4v3 64 1 IFA IFA NNP cord-308950-bl83r4v3 64 2 and and CC cord-308950-bl83r4v3 64 3 confocal confocal JJ cord-308950-bl83r4v3 64 4 analyses analysis NNS cord-308950-bl83r4v3 64 5 of of IN cord-308950-bl83r4v3 64 6 FEK FEK NNP cord-308950-bl83r4v3 64 7 cells cell NNS cord-308950-bl83r4v3 64 8 inoculated inoculate VBN cord-308950-bl83r4v3 64 9 with with IN cord-308950-bl83r4v3 64 10 Ark Ark NNP cord-308950-bl83r4v3 64 11 / / SYM cord-308950-bl83r4v3 64 12 IBV IBV NNP cord-308950-bl83r4v3 64 13 demonstrated demonstrate VBD cord-308950-bl83r4v3 64 14 that that IN cord-308950-bl83r4v3 64 15 the the DT cord-308950-bl83r4v3 64 16 FEK FEK NNP cord-308950-bl83r4v3 64 17 cells cell NNS cord-308950-bl83r4v3 64 18 were be VBD cord-308950-bl83r4v3 64 19 susceptible susceptible JJ cord-308950-bl83r4v3 64 20 to to IN cord-308950-bl83r4v3 64 21 the the DT cord-308950-bl83r4v3 64 22 virus virus NN cord-308950-bl83r4v3 64 23 . . . cord-308950-bl83r4v3 65 1 Fluorescence fluorescence NN cord-308950-bl83r4v3 65 2 was be VBD cord-308950-bl83r4v3 65 3 detected detect VBN cord-308950-bl83r4v3 65 4 on on IN cord-308950-bl83r4v3 65 5 the the DT cord-308950-bl83r4v3 65 6 surface surface NN cord-308950-bl83r4v3 65 7 of of IN cord-308950-bl83r4v3 65 8 inoculated inoculate VBN cord-308950-bl83r4v3 65 9 cells cell NNS cord-308950-bl83r4v3 65 10 and and CC cord-308950-bl83r4v3 65 11 the the DT cord-308950-bl83r4v3 65 12 accumulation accumulation NN cord-308950-bl83r4v3 65 13 of of IN cord-308950-bl83r4v3 65 14 viral viral JJ cord-308950-bl83r4v3 65 15 antigen antigen NN cord-308950-bl83r4v3 65 16 in in IN cord-308950-bl83r4v3 65 17 the the DT cord-308950-bl83r4v3 65 18 cytoplasm cytoplasm NN cord-308950-bl83r4v3 65 19 , , , cord-308950-bl83r4v3 65 20 the the DT cord-308950-bl83r4v3 65 21 site site NN cord-308950-bl83r4v3 65 22 of of IN cord-308950-bl83r4v3 65 23 replication replication NN cord-308950-bl83r4v3 65 24 for for IN cord-308950-bl83r4v3 65 25 IBV IBV NNP cord-308950-bl83r4v3 65 26 , , , cord-308950-bl83r4v3 65 27 was be VBD cord-308950-bl83r4v3 65 28 detected detect VBN cord-308950-bl83r4v3 65 29 ( ( -LRB- cord-308950-bl83r4v3 65 30 Figs fig NNS cord-308950-bl83r4v3 65 31 . . . cord-308950-bl83r4v3 66 1 1A 1A NNP cord-308950-bl83r4v3 66 2 and and CC cord-308950-bl83r4v3 66 3 1E 1E NNP cord-308950-bl83r4v3 66 4 ) ) -RRB- cord-308950-bl83r4v3 66 5 . . . cord-308950-bl83r4v3 67 1 Viral viral JJ cord-308950-bl83r4v3 67 2 antigen antigen NN cord-308950-bl83r4v3 67 3 was be VBD cord-308950-bl83r4v3 67 4 detected detect VBN cord-308950-bl83r4v3 67 5 as as RB cord-308950-bl83r4v3 67 6 soon soon RB cord-308950-bl83r4v3 67 7 as as IN cord-308950-bl83r4v3 67 8 1 1 CD cord-308950-bl83r4v3 67 9 h h NN cord-308950-bl83r4v3 67 10 post post NN cord-308950-bl83r4v3 67 11 infection infection NN cord-308950-bl83r4v3 67 12 ( ( -LRB- cord-308950-bl83r4v3 67 13 PI PI NNP cord-308950-bl83r4v3 67 14 ) ) -RRB- cord-308950-bl83r4v3 67 15 . . . cord-308950-bl83r4v3 68 1 The the DT cord-308950-bl83r4v3 68 2 BHK-21 BHK-21 NNP cord-308950-bl83r4v3 68 3 cells cell NNS cord-308950-bl83r4v3 68 4 , , , cord-308950-bl83r4v3 68 5 which which WDT cord-308950-bl83r4v3 68 6 were be VBD cord-308950-bl83r4v3 68 7 shown show VBN cord-308950-bl83r4v3 68 8 to to TO cord-308950-bl83r4v3 68 9 be be VB cord-308950-bl83r4v3 68 10 non non JJ cord-308950-bl83r4v3 68 11 - - JJ cord-308950-bl83r4v3 68 12 permissive permissive JJ cord-308950-bl83r4v3 68 13 to to IN cord-308950-bl83r4v3 68 14 IBV IBV NNP cord-308950-bl83r4v3 68 15 infection infection NN cord-308950-bl83r4v3 68 16 by by IN cord-308950-bl83r4v3 68 17 IFA IFA NNP cord-308950-bl83r4v3 68 18 , , , cord-308950-bl83r4v3 68 19 were be VBD cord-308950-bl83r4v3 68 20 used use VBN cord-308950-bl83r4v3 68 21 as as IN cord-308950-bl83r4v3 68 22 a a DT cord-308950-bl83r4v3 68 23 negative negative JJ cord-308950-bl83r4v3 68 24 control control NN cord-308950-bl83r4v3 68 25 cell cell NN cord-308950-bl83r4v3 68 26 line line NN cord-308950-bl83r4v3 68 27 ( ( -LRB- cord-308950-bl83r4v3 68 28 Figs fig NNS cord-308950-bl83r4v3 68 29 . . . cord-308950-bl83r4v3 69 1 1B 1B NNP cord-308950-bl83r4v3 69 2 and and CC cord-308950-bl83r4v3 69 3 IF IF NNP cord-308950-bl83r4v3 69 4 ) ) -RRB- cord-308950-bl83r4v3 69 5 . . . cord-308950-bl83r4v3 70 1 To to TO cord-308950-bl83r4v3 70 2 determine determine VB cord-308950-bl83r4v3 70 3 if if IN cord-308950-bl83r4v3 70 4 IBV IBV NNP cord-308950-bl83r4v3 70 5 could could MD cord-308950-bl83r4v3 70 6 replicate replicate VB cord-308950-bl83r4v3 70 7 in in IN cord-308950-bl83r4v3 70 8 the the DT cord-308950-bl83r4v3 70 9 FEK FEK NNP cord-308950-bl83r4v3 70 10 cells cell NNS cord-308950-bl83r4v3 70 11 , , , cord-308950-bl83r4v3 70 12 cell cell NN cord-308950-bl83r4v3 70 13 cultures culture NNS cord-308950-bl83r4v3 70 14 were be VBD cord-308950-bl83r4v3 70 15 infected infect VBN cord-308950-bl83r4v3 70 16 with with IN cord-308950-bl83r4v3 70 17 Ark Ark NNP cord-308950-bl83r4v3 70 18 / / SYM cord-308950-bl83r4v3 70 19 IBV IBV NNP cord-308950-bl83r4v3 70 20 and and CC cord-308950-bl83r4v3 70 21 the the DT cord-308950-bl83r4v3 70 22 supernatants supernatant NNS cord-308950-bl83r4v3 70 23 from from IN cord-308950-bl83r4v3 70 24 infected infected JJ cord-308950-bl83r4v3 70 25 cells cell NNS cord-308950-bl83r4v3 70 26 were be VBD cord-308950-bl83r4v3 70 27 collected collect VBN cord-308950-bl83r4v3 70 28 at at IN cord-308950-bl83r4v3 70 29 1 1 CD cord-308950-bl83r4v3 70 30 , , , cord-308950-bl83r4v3 70 31 2 2 CD cord-308950-bl83r4v3 70 32 , , , cord-308950-bl83r4v3 70 33 3 3 CD cord-308950-bl83r4v3 70 34 and and CC cord-308950-bl83r4v3 70 35 10 10 CD cord-308950-bl83r4v3 70 36 d d NN cord-308950-bl83r4v3 70 37 PI pi NN cord-308950-bl83r4v3 70 38 . . . cord-308950-bl83r4v3 71 1 The the DT cord-308950-bl83r4v3 71 2 cell cell NN cord-308950-bl83r4v3 71 3 supernatants supernatant NNS cord-308950-bl83r4v3 71 4 were be VBD cord-308950-bl83r4v3 71 5 titrated titrate VBN cord-308950-bl83r4v3 71 6 in in IN cord-308950-bl83r4v3 71 7 chicken chicken NN cord-308950-bl83r4v3 71 8 embryos embryo NNS cord-308950-bl83r4v3 71 9 and and CC cord-308950-bl83r4v3 71 10 the the DT cord-308950-bl83r4v3 71 11 EID EID NNP cord-308950-bl83r4v3 71 12 50 50 CD cord-308950-bl83r4v3 71 13 was be VBD cord-308950-bl83r4v3 71 14 calculated calculate VBN cord-308950-bl83r4v3 71 15 . . . cord-308950-bl83r4v3 72 1 The the DT cord-308950-bl83r4v3 72 2 results result NNS cord-308950-bl83r4v3 72 3 indicated indicate VBD cord-308950-bl83r4v3 72 4 that that IN cord-308950-bl83r4v3 72 5 Ark Ark NNP cord-308950-bl83r4v3 72 6 / / SYM cord-308950-bl83r4v3 72 7 IBV IBV NNP cord-308950-bl83r4v3 72 8 does do VBZ cord-308950-bl83r4v3 72 9 replicate replicate VB cord-308950-bl83r4v3 72 10 in in IN cord-308950-bl83r4v3 72 11 the the DT cord-308950-bl83r4v3 72 12 FEK FEK NNP cord-308950-bl83r4v3 72 13 cells cell NNS cord-308950-bl83r4v3 72 14 in in IN cord-308950-bl83r4v3 72 15 vitro vitro FW cord-308950-bl83r4v3 72 16 . . . cord-308950-bl83r4v3 73 1 The the DT cord-308950-bl83r4v3 73 2 EID EID NNP cord-308950-bl83r4v3 73 3 50 50 CD cord-308950-bl83r4v3 74 1 /ml /ml . cord-308950-bl83r4v3 75 1 values value NNS cord-308950-bl83r4v3 75 2 were be VBD cord-308950-bl83r4v3 75 3 10 10 CD cord-308950-bl83r4v3 75 4 3.8 3.8 CD cord-308950-bl83r4v3 75 5 , , , cord-308950-bl83r4v3 75 6 10 10 CD cord-308950-bl83r4v3 75 7 5.8 5.8 CD cord-308950-bl83r4v3 75 8 , , , cord-308950-bl83r4v3 75 9 10 10 CD cord-308950-bl83r4v3 75 10 7.2 7.2 CD cord-308950-bl83r4v3 75 11 and and CC cord-308950-bl83r4v3 75 12 10 10 CD cord-308950-bl83r4v3 75 13 9.2 9.2 CD cord-308950-bl83r4v3 75 14 for for IN cord-308950-bl83r4v3 75 15 the the DT cord-308950-bl83r4v3 75 16 1 1 CD cord-308950-bl83r4v3 75 17 , , , cord-308950-bl83r4v3 75 18 2 2 CD cord-308950-bl83r4v3 75 19 , , , cord-308950-bl83r4v3 75 20 3 3 CD cord-308950-bl83r4v3 75 21 , , , cord-308950-bl83r4v3 75 22 and and CC cord-308950-bl83r4v3 75 23 10 10 CD cord-308950-bl83r4v3 75 24 d d NN cord-308950-bl83r4v3 75 25 incubation incubation NN cord-308950-bl83r4v3 75 26 , , , cord-308950-bl83r4v3 75 27 respectively respectively RB cord-308950-bl83r4v3 75 28 . . . cord-308950-bl83r4v3 76 1 The the DT cord-308950-bl83r4v3 76 2 pathological pathological JJ cord-308950-bl83r4v3 76 3 lesions lesion NNS cord-308950-bl83r4v3 76 4 , , , cord-308950-bl83r4v3 76 5 including include VBG cord-308950-bl83r4v3 76 6 dwarfed dwarfed JJ cord-308950-bl83r4v3 76 7 and and CC cord-308950-bl83r4v3 76 8 hemorrhagic hemorrhagic JJ cord-308950-bl83r4v3 76 9 embryos embryo NNS cord-308950-bl83r4v3 76 10 with with IN cord-308950-bl83r4v3 76 11 curled curled JJ cord-308950-bl83r4v3 76 12 toes toe NNS cord-308950-bl83r4v3 76 13 typically typically RB cord-308950-bl83r4v3 76 14 present present JJ cord-308950-bl83r4v3 76 15 in in IN cord-308950-bl83r4v3 76 16 IBV IBV NNP cord-308950-bl83r4v3 76 17 infected infect VBN cord-308950-bl83r4v3 76 18 embryos embryo NNS cord-308950-bl83r4v3 76 19 [ [ -LRB- cord-308950-bl83r4v3 76 20 40 40 CD cord-308950-bl83r4v3 76 21 ] ] -RRB- cord-308950-bl83r4v3 76 22 were be VBD cord-308950-bl83r4v3 76 23 produced produce VBN cord-308950-bl83r4v3 76 24 in in IN cord-308950-bl83r4v3 76 25 SPF SPF NNP cord-308950-bl83r4v3 76 26 embryonated embryonate VBN cord-308950-bl83r4v3 76 27 chicken chicken NN cord-308950-bl83r4v3 76 28 eggs egg NNS cord-308950-bl83r4v3 76 29 infected infect VBN cord-308950-bl83r4v3 76 30 with with IN cord-308950-bl83r4v3 76 31 virus virus NN cord-308950-bl83r4v3 76 32 passaged passage VBN cord-308950-bl83r4v3 76 33 in in IN cord-308950-bl83r4v3 76 34 FEK FEK NNP cord-308950-bl83r4v3 76 35 cells cell NNS cord-308950-bl83r4v3 76 36 ( ( -LRB- cord-308950-bl83r4v3 76 37 Fig fig NN cord-308950-bl83r4v3 76 38 . . NNP cord-308950-bl83r4v3 76 39 2 2 CD cord-308950-bl83r4v3 76 40 ) ) -RRB- cord-308950-bl83r4v3 76 41 . . . cord-308950-bl83r4v3 76 42 _SP cord-308950-bl83r4v3 77 1 Previous previous JJ cord-308950-bl83r4v3 77 2 reports report NNS cord-308950-bl83r4v3 77 3 have have VBP cord-308950-bl83r4v3 77 4 shown show VBN cord-308950-bl83r4v3 77 5 fAPN fapn NN cord-308950-bl83r4v3 77 6 to to TO cord-308950-bl83r4v3 77 7 be be VB cord-308950-bl83r4v3 77 8 the the DT cord-308950-bl83r4v3 77 9 molecule molecule NN cord-308950-bl83r4v3 77 10 that that WDT cord-308950-bl83r4v3 77 11 allows allow VBZ cord-308950-bl83r4v3 77 12 entry entry NN cord-308950-bl83r4v3 77 13 of of IN cord-308950-bl83r4v3 77 14 the the DT cord-308950-bl83r4v3 77 15 coronavirus coronavirus NN cord-308950-bl83r4v3 77 16 from from IN cord-308950-bl83r4v3 77 17 other other JJ cord-308950-bl83r4v3 77 18 species specie NNS cord-308950-bl83r4v3 77 19 into into IN cord-308950-bl83r4v3 77 20 the the DT cord-308950-bl83r4v3 77 21 feline feline JJ cord-308950-bl83r4v3 77 22 cells cell NNS cord-308950-bl83r4v3 77 23 [ [ -LRB- cord-308950-bl83r4v3 77 24 38 38 CD cord-308950-bl83r4v3 77 25 , , , cord-308950-bl83r4v3 77 26 39 39 CD cord-308950-bl83r4v3 77 27 ] ] -RRB- cord-308950-bl83r4v3 77 28 . . . cord-308950-bl83r4v3 78 1 To to TO cord-308950-bl83r4v3 78 2 test test VB cord-308950-bl83r4v3 78 3 the the DT cord-308950-bl83r4v3 78 4 hypothesis hypothesis NN cord-308950-bl83r4v3 78 5 that that IN cord-308950-bl83r4v3 78 6 the the DT cord-308950-bl83r4v3 78 7 feline feline JJ cord-308950-bl83r4v3 78 8 APN APN NNP cord-308950-bl83r4v3 78 9 had have VBD cord-308950-bl83r4v3 78 10 a a DT cord-308950-bl83r4v3 78 11 role role NN cord-308950-bl83r4v3 78 12 in in IN cord-308950-bl83r4v3 78 13 permissiveness permissiveness NN cord-308950-bl83r4v3 78 14 of of IN cord-308950-bl83r4v3 78 15 FEK FEK NNP cord-308950-bl83r4v3 78 16 cells cell NNS cord-308950-bl83r4v3 78 17 to to IN cord-308950-bl83r4v3 78 18 Ark Ark NNP cord-308950-bl83r4v3 78 19 / / SYM cord-308950-bl83r4v3 78 20 IBV IBV NNP cord-308950-bl83r4v3 78 21 , , , cord-308950-bl83r4v3 78 22 BHK-21 BHK-21 NNP cord-308950-bl83r4v3 78 23 cells cell NNS cord-308950-bl83r4v3 78 24 were be VBD cord-308950-bl83r4v3 78 25 transfected transfecte VBN cord-308950-bl83r4v3 78 26 with with IN cord-308950-bl83r4v3 78 27 pBK pBK NNP cord-308950-bl83r4v3 78 28 - - HYPH cord-308950-bl83r4v3 78 29 CMV cmv NN cord-308950-bl83r4v3 78 30 plasmid plasmid NN cord-308950-bl83r4v3 78 31 or or CC cord-308950-bl83r4v3 78 32 pBK pbk NN cord-308950-bl83r4v3 78 33 - - HYPH cord-308950-bl83r4v3 78 34 fAPN fapn NN cord-308950-bl83r4v3 78 35 . . . cord-308950-bl83r4v3 78 36 Surface surface NN cord-308950-bl83r4v3 78 37 expression expression NN cord-308950-bl83r4v3 78 38 of of IN cord-308950-bl83r4v3 78 39 fAPN fapn NN cord-308950-bl83r4v3 78 40 was be VBD cord-308950-bl83r4v3 78 41 assayed assay VBN cord-308950-bl83r4v3 78 42 by by IN cord-308950-bl83r4v3 78 43 flow flow NN cord-308950-bl83r4v3 78 44 cytometry cytometry NN cord-308950-bl83r4v3 78 45 . . . cord-308950-bl83r4v3 79 1 The the DT cord-308950-bl83r4v3 79 2 BHK-21 BHK-21 NNP cord-308950-bl83r4v3 79 3 cells cell NNS cord-308950-bl83r4v3 79 4 transfected transfecte VBN cord-308950-bl83r4v3 79 5 with with IN cord-308950-bl83r4v3 79 6 pBK pBK NNP cord-308950-bl83r4v3 79 7 - - HYPH cord-308950-bl83r4v3 79 8 fAPN fapn NN cord-308950-bl83r4v3 79 9 were be VBD cord-308950-bl83r4v3 79 10 shown show VBN cord-308950-bl83r4v3 79 11 to to TO cord-308950-bl83r4v3 79 12 express express VB cord-308950-bl83r4v3 79 13 the the DT cord-308950-bl83r4v3 79 14 fAPN fAPN NNP cord-308950-bl83r4v3 79 15 receptor receptor NN cord-308950-bl83r4v3 79 16 on on IN cord-308950-bl83r4v3 79 17 the the DT cord-308950-bl83r4v3 79 18 cell cell NN cord-308950-bl83r4v3 79 19 surface surface NN cord-308950-bl83r4v3 79 20 ( ( -LRB- cord-308950-bl83r4v3 79 21 Fig Fig NNP cord-308950-bl83r4v3 79 22 . . . cord-308950-bl83r4v3 79 23 3 3 CD cord-308950-bl83r4v3 79 24 ) ) -RRB- cord-308950-bl83r4v3 79 25 , , , cord-308950-bl83r4v3 79 26 and and CC cord-308950-bl83r4v3 79 27 were be VBD cord-308950-bl83r4v3 79 28 permissive permissive JJ cord-308950-bl83r4v3 79 29 to to IN cord-308950-bl83r4v3 79 30 Ark Ark NNP cord-308950-bl83r4v3 79 31 / / SYM cord-308950-bl83r4v3 79 32 IBV IBV NNP cord-308950-bl83r4v3 79 33 ( ( -LRB- cord-308950-bl83r4v3 79 34 Figs Figs NNP cord-308950-bl83r4v3 79 35 . . . cord-308950-bl83r4v3 80 1 1C 1C NNP cord-308950-bl83r4v3 80 2 and and CC cord-308950-bl83r4v3 80 3 1 1 CD cord-308950-bl83r4v3 80 4 G G NNP cord-308950-bl83r4v3 80 5 ) ) -RRB- cord-308950-bl83r4v3 80 6 . . . cord-308950-bl83r4v3 81 1 Viral viral JJ cord-308950-bl83r4v3 81 2 antigen antigen NN cord-308950-bl83r4v3 81 3 labeled label VBN cord-308950-bl83r4v3 81 4 with with IN cord-308950-bl83r4v3 81 5 fluorescein fluorescein NNP cord-308950-bl83r4v3 81 6 was be VBD cord-308950-bl83r4v3 81 7 detected detect VBN cord-308950-bl83r4v3 81 8 in in IN cord-308950-bl83r4v3 81 9 the the DT cord-308950-bl83r4v3 81 10 cell cell NN cord-308950-bl83r4v3 81 11 cytoplasm cytoplasm NN cord-308950-bl83r4v3 81 12 , , , cord-308950-bl83r4v3 81 13 and and CC cord-308950-bl83r4v3 81 14 further further JJ cord-308950-bl83r4v3 81 15 examination examination NN cord-308950-bl83r4v3 81 16 of of IN cord-308950-bl83r4v3 81 17 the the DT cord-308950-bl83r4v3 81 18 cells cell NNS cord-308950-bl83r4v3 81 19 with with IN cord-308950-bl83r4v3 81 20 confocal confocal JJ cord-308950-bl83r4v3 81 21 microscopy microscopy NNS cord-308950-bl83r4v3 81 22 also also RB cord-308950-bl83r4v3 81 23 showed show VBD cord-308950-bl83r4v3 81 24 that that IN cord-308950-bl83r4v3 81 25 the the DT cord-308950-bl83r4v3 81 26 virus virus NN cord-308950-bl83r4v3 81 27 was be VBD cord-308950-bl83r4v3 81 28 intracytoplasmic intracytoplasmic JJ cord-308950-bl83r4v3 81 29 and and CC cord-308950-bl83r4v3 81 30 not not RB cord-308950-bl83r4v3 81 31 membrane membrane NN cord-308950-bl83r4v3 81 32 bound bind VBN cord-308950-bl83r4v3 81 33 ( ( -LRB- cord-308950-bl83r4v3 81 34 Fig fig NN cord-308950-bl83r4v3 81 35 . . . cord-308950-bl83r4v3 81 36 1 1 CD cord-308950-bl83r4v3 81 37 G g NN cord-308950-bl83r4v3 81 38 ) ) -RRB- cord-308950-bl83r4v3 81 39 . . . cord-308950-bl83r4v3 82 1 However however RB cord-308950-bl83r4v3 82 2 , , , cord-308950-bl83r4v3 82 3 fluorescence fluorescence NN cord-308950-bl83r4v3 82 4 was be VBD cord-308950-bl83r4v3 82 5 not not RB cord-308950-bl83r4v3 82 6 detected detect VBN cord-308950-bl83r4v3 82 7 on on IN cord-308950-bl83r4v3 82 8 the the DT cord-308950-bl83r4v3 82 9 surface surface NN cord-308950-bl83r4v3 82 10 or or CC cord-308950-bl83r4v3 82 11 in in IN cord-308950-bl83r4v3 82 12 cytoplasm cytoplasm NN cord-308950-bl83r4v3 82 13 of of IN cord-308950-bl83r4v3 82 14 BHK BHK NNP cord-308950-bl83r4v3 82 15 cells cell NNS cord-308950-bl83r4v3 82 16 transfected transfecte VBN cord-308950-bl83r4v3 82 17 with with IN cord-308950-bl83r4v3 82 18 pBK pBK NNP cord-308950-bl83r4v3 82 19 - - HYPH cord-308950-bl83r4v3 82 20 CMV CMV NNP cord-308950-bl83r4v3 82 21 ( ( -LRB- cord-308950-bl83r4v3 82 22 Figs fig NNS cord-308950-bl83r4v3 82 23 . . . cord-308950-bl83r4v3 83 1 1D 1D NNP cord-308950-bl83r4v3 83 2 and and CC cord-308950-bl83r4v3 83 3 1H 1h CD cord-308950-bl83r4v3 83 4 ) ) -RRB- cord-308950-bl83r4v3 83 5 . . . cord-308950-bl83r4v3 84 1 This this DT cord-308950-bl83r4v3 84 2 study study NN cord-308950-bl83r4v3 84 3 showed show VBD cord-308950-bl83r4v3 84 4 that that IN cord-308950-bl83r4v3 84 5 FEK FEK NNP cord-308950-bl83r4v3 84 6 cells cell NNS cord-308950-bl83r4v3 84 7 in in IN cord-308950-bl83r4v3 84 8 culture culture NN cord-308950-bl83r4v3 84 9 were be VBD cord-308950-bl83r4v3 84 10 permissive permissive JJ cord-308950-bl83r4v3 84 11 to to IN cord-308950-bl83r4v3 84 12 Ark Ark NNP cord-308950-bl83r4v3 84 13 / / SYM cord-308950-bl83r4v3 84 14 IBV IBV NNP cord-308950-bl83r4v3 84 15 and and CC cord-308950-bl83r4v3 84 16 the the DT cord-308950-bl83r4v3 84 17 virus virus NN cord-308950-bl83r4v3 84 18 was be VBD cord-308950-bl83r4v3 84 19 capable capable JJ cord-308950-bl83r4v3 84 20 of of IN cord-308950-bl83r4v3 84 21 replicating replicate VBG cord-308950-bl83r4v3 84 22 within within IN cord-308950-bl83r4v3 84 23 the the DT cord-308950-bl83r4v3 84 24 cells cell NNS cord-308950-bl83r4v3 84 25 . . . cord-308950-bl83r4v3 85 1 Previous previous JJ cord-308950-bl83r4v3 85 2 studies study NNS cord-308950-bl83r4v3 85 3 have have VBP cord-308950-bl83r4v3 85 4 shown show VBN cord-308950-bl83r4v3 85 5 that that IN cord-308950-bl83r4v3 85 6 cats cat NNS cord-308950-bl83r4v3 85 7 may may MD cord-308950-bl83r4v3 85 8 become become VB cord-308950-bl83r4v3 85 9 infected infect VBN cord-308950-bl83r4v3 85 10 with with IN cord-308950-bl83r4v3 85 11 coronavirus coronavirus NN cord-308950-bl83r4v3 85 12 from from IN cord-308950-bl83r4v3 85 13 other other JJ cord-308950-bl83r4v3 85 14 animal animal NN cord-308950-bl83r4v3 85 15 species specie NNS cord-308950-bl83r4v3 85 16 and and CC cord-308950-bl83r4v3 85 17 seroconvert seroconvert VB cord-308950-bl83r4v3 85 18 without without IN cord-308950-bl83r4v3 85 19 developing develop VBG cord-308950-bl83r4v3 85 20 clinical clinical JJ cord-308950-bl83r4v3 85 21 signs sign NNS cord-308950-bl83r4v3 85 22 of of IN cord-308950-bl83r4v3 85 23 disease disease NN cord-308950-bl83r4v3 85 24 [ [ -LRB- cord-308950-bl83r4v3 85 25 4 4 CD cord-308950-bl83r4v3 85 26 , , , cord-308950-bl83r4v3 85 27 5 5 CD cord-308950-bl83r4v3 85 28 ] ] -RRB- cord-308950-bl83r4v3 85 29 polymerases polymerase NNS cord-308950-bl83r4v3 85 30 , , , cord-308950-bl83r4v3 85 31 and and CC cord-308950-bl83r4v3 85 32 therefore therefore RB cord-308950-bl83r4v3 85 33 may may MD cord-308950-bl83r4v3 85 34 undergo undergo VB cord-308950-bl83r4v3 85 35 recombination recombination NN cord-308950-bl83r4v3 85 36 [ [ -LRB- cord-308950-bl83r4v3 85 37 3 3 CD cord-308950-bl83r4v3 85 38 , , , cord-308950-bl83r4v3 85 39 13 13 CD cord-308950-bl83r4v3 85 40 , , , cord-308950-bl83r4v3 85 41 17 17 CD cord-308950-bl83r4v3 85 42 ] ] -RRB- cord-308950-bl83r4v3 85 43 in in IN cord-308950-bl83r4v3 85 44 cats cat NNS cord-308950-bl83r4v3 85 45 coinfected coinfecte VBD cord-308950-bl83r4v3 85 46 with with IN cord-308950-bl83r4v3 85 47 different different JJ cord-308950-bl83r4v3 85 48 coronaviruses coronaviruse NNS cord-308950-bl83r4v3 85 49 . . . cord-308950-bl83r4v3 86 1 The the DT cord-308950-bl83r4v3 86 2 resulting result VBG cord-308950-bl83r4v3 86 3 recombinant recombinant JJ cord-308950-bl83r4v3 86 4 viruses virus NNS cord-308950-bl83r4v3 86 5 may may MD cord-308950-bl83r4v3 86 6 have have VB cord-308950-bl83r4v3 86 7 properties property NNS cord-308950-bl83r4v3 86 8 different different JJ cord-308950-bl83r4v3 86 9 from from IN cord-308950-bl83r4v3 86 10 either either DT cord-308950-bl83r4v3 86 11 parent parent NN cord-308950-bl83r4v3 86 12 , , , cord-308950-bl83r4v3 86 13 infect infect VBP cord-308950-bl83r4v3 86 14 different different JJ cord-308950-bl83r4v3 86 15 hosts host NNS cord-308950-bl83r4v3 86 16 and and CC cord-308950-bl83r4v3 86 17 have have VBP cord-308950-bl83r4v3 86 18 different different JJ cord-308950-bl83r4v3 86 19 tissue tissue NN cord-308950-bl83r4v3 86 20 tropism tropism NN cord-308950-bl83r4v3 86 21 , , , cord-308950-bl83r4v3 86 22 antigenicity antigenicity NN cord-308950-bl83r4v3 86 23 and and CC cord-308950-bl83r4v3 86 24 virulence virulence NN cord-308950-bl83r4v3 86 25 , , , cord-308950-bl83r4v3 86 26 possibly possibly RB cord-308950-bl83r4v3 86 27 resulting result VBG cord-308950-bl83r4v3 86 28 in in IN cord-308950-bl83r4v3 86 29 the the DT cord-308950-bl83r4v3 86 30 emergence emergence NN cord-308950-bl83r4v3 86 31 of of IN cord-308950-bl83r4v3 86 32 a a DT cord-308950-bl83r4v3 86 33 new new JJ cord-308950-bl83r4v3 86 34 disease disease NN cord-308950-bl83r4v3 86 35 [ [ -LRB- cord-308950-bl83r4v3 86 36 38 38 CD cord-308950-bl83r4v3 86 37 ] ] -RRB- cord-308950-bl83r4v3 86 38 . . . cord-308950-bl83r4v3 87 1 Because because IN cord-308950-bl83r4v3 87 2 feline feline JJ cord-308950-bl83r4v3 87 3 cells cell NNS cord-308950-bl83r4v3 87 4 can can MD cord-308950-bl83r4v3 87 5 harbor harbor VB cord-308950-bl83r4v3 87 6 coronaviruses coronaviruse NNS cord-308950-bl83r4v3 87 7 from from IN cord-308950-bl83r4v3 87 8 several several JJ cord-308950-bl83r4v3 87 9 species specie NNS cord-308950-bl83r4v3 87 10 , , , cord-308950-bl83r4v3 87 11 this this DT cord-308950-bl83r4v3 87 12 may may MD cord-308950-bl83r4v3 87 13 explain explain VB cord-308950-bl83r4v3 87 14 why why WRB cord-308950-bl83r4v3 87 15 recombination recombination NN cord-308950-bl83r4v3 87 16 of of IN cord-308950-bl83r4v3 87 17 coronaviruses coronaviruse NNS cord-308950-bl83r4v3 87 18 occurs occur VBZ cord-308950-bl83r4v3 87 19 somewhat somewhat RB cord-308950-bl83r4v3 87 20 frequently frequently RB cord-308950-bl83r4v3 87 21 . . . cord-308950-bl83r4v3 88 1 Cats cat NNS cord-308950-bl83r4v3 88 2 may may MD cord-308950-bl83r4v3 88 3 serve serve VB cord-308950-bl83r4v3 88 4 as as IN cord-308950-bl83r4v3 88 5 a a DT cord-308950-bl83r4v3 88 6 vehicle vehicle NN cord-308950-bl83r4v3 88 7 for for IN cord-308950-bl83r4v3 88 8 the the DT cord-308950-bl83r4v3 88 9 recombination recombination NN cord-308950-bl83r4v3 88 10 event event NN cord-308950-bl83r4v3 88 11 and and CC cord-308950-bl83r4v3 88 12 may may MD cord-308950-bl83r4v3 88 13 contribute contribute VB cord-308950-bl83r4v3 88 14 to to IN cord-308950-bl83r4v3 88 15 new new JJ cord-308950-bl83r4v3 88 16 IBV IBV NNP cord-308950-bl83r4v3 88 17 emerging emerge VBG cord-308950-bl83r4v3 88 18 serotypes serotype NNS cord-308950-bl83r4v3 88 19 . . . cord-308950-bl83r4v3 89 1 There there EX cord-308950-bl83r4v3 89 2 have have VBP cord-308950-bl83r4v3 89 3 been be VBN cord-308950-bl83r4v3 89 4 more more JJR cord-308950-bl83r4v3 89 5 than than IN cord-308950-bl83r4v3 89 6 20 20 CD cord-308950-bl83r4v3 89 7 serotypes serotype NNS cord-308950-bl83r4v3 89 8 of of IN cord-308950-bl83r4v3 89 9 IBV IBV NNP cord-308950-bl83r4v3 89 10 identified identify VBN cord-308950-bl83r4v3 89 11 and and CC cord-308950-bl83r4v3 89 12 new new JJ cord-308950-bl83r4v3 89 13 IBV IBV NNP cord-308950-bl83r4v3 89 14 variants variant NNS cord-308950-bl83r4v3 89 15 continue continue VBP cord-308950-bl83r4v3 89 16 to to TO cord-308950-bl83r4v3 89 17 emerge emerge VB cord-308950-bl83r4v3 89 18 . . . cord-308950-bl83r4v3 90 1 Recombination recombination NN cord-308950-bl83r4v3 90 2 has have VBZ cord-308950-bl83r4v3 90 3 been be VBN cord-308950-bl83r4v3 90 4 experimentally experimentally RB cord-308950-bl83r4v3 90 5 proven prove VBN cord-308950-bl83r4v3 90 6 to to TO cord-308950-bl83r4v3 90 7 occur occur VB cord-308950-bl83r4v3 90 8 in in IN cord-308950-bl83r4v3 90 9 IBV IBV NNP cord-308950-bl83r4v3 90 10 [ [ -LRB- cord-308950-bl83r4v3 90 11 19 19 CD cord-308950-bl83r4v3 90 12 , , , cord-308950-bl83r4v3 90 13 24 24 CD cord-308950-bl83r4v3 90 14 , , , cord-308950-bl83r4v3 90 15 25 25 CD cord-308950-bl83r4v3 90 16 , , , cord-308950-bl83r4v3 90 17 27 27 CD cord-308950-bl83r4v3 90 18 , , , cord-308950-bl83r4v3 90 19 29 29 CD cord-308950-bl83r4v3 90 20 , , , cord-308950-bl83r4v3 90 21 41 41 CD cord-308950-bl83r4v3 90 22 ] ] -RRB- cord-308950-bl83r4v3 90 23 particularly particularly RB cord-308950-bl83r4v3 90 24 in in IN cord-308950-bl83r4v3 90 25 the the DT cord-308950-bl83r4v3 90 26 hypervariable hypervariable JJ cord-308950-bl83r4v3 90 27 region region NN cord-308950-bl83r4v3 90 28 of of IN cord-308950-bl83r4v3 90 29 S1 S1 NNP cord-308950-bl83r4v3 90 30 [ [ -LRB- cord-308950-bl83r4v3 90 31 42 42 CD cord-308950-bl83r4v3 90 32 ] ] -RRB- cord-308950-bl83r4v3 90 33 . . . cord-308950-bl83r4v3 91 1 This this DT cord-308950-bl83r4v3 91 2 region region NN cord-308950-bl83r4v3 91 3 of of IN cord-308950-bl83r4v3 91 4 the the DT cord-308950-bl83r4v3 91 5 S1 S1 NNP cord-308950-bl83r4v3 91 6 glycoprotein glycoprotein NN cord-308950-bl83r4v3 91 7 of of IN cord-308950-bl83r4v3 91 8 IBV IBV NNP cord-308950-bl83r4v3 91 9 has have VBZ cord-308950-bl83r4v3 91 10 also also RB cord-308950-bl83r4v3 91 11 been be VBN cord-308950-bl83r4v3 91 12 postulated postulate VBN cord-308950-bl83r4v3 91 13 to to TO cord-308950-bl83r4v3 91 14 be be VB cord-308950-bl83r4v3 91 15 one one CD cord-308950-bl83r4v3 91 16 of of IN cord-308950-bl83r4v3 91 17 the the DT cord-308950-bl83r4v3 91 18 major major JJ cord-308950-bl83r4v3 91 19 elicitors elicitor NNS cord-308950-bl83r4v3 91 20 of of IN cord-308950-bl83r4v3 91 21 the the DT cord-308950-bl83r4v3 91 22 immune immune JJ cord-308950-bl83r4v3 91 23 response response NN cord-308950-bl83r4v3 91 24 [ [ -LRB- cord-308950-bl83r4v3 91 25 18 18 CD cord-308950-bl83r4v3 91 26 , , , cord-308950-bl83r4v3 91 27 20 20 CD cord-308950-bl83r4v3 91 28 , , , cord-308950-bl83r4v3 91 29 37 37 CD cord-308950-bl83r4v3 91 30 ] ] -RRB- cord-308950-bl83r4v3 91 31 . . . cord-308950-bl83r4v3 92 1 Consequently consequently RB cord-308950-bl83r4v3 92 2 , , , cord-308950-bl83r4v3 92 3 current current JJ cord-308950-bl83r4v3 92 4 vaccines vaccine NNS cord-308950-bl83r4v3 92 5 generally generally RB cord-308950-bl83r4v3 92 6 do do VBP cord-308950-bl83r4v3 92 7 not not RB cord-308950-bl83r4v3 92 8 cross cross VB cord-308950-bl83r4v3 92 9 protect protect VB cord-308950-bl83r4v3 92 10 against against IN cord-308950-bl83r4v3 92 11 the the DT cord-308950-bl83r4v3 92 12 different different JJ cord-308950-bl83r4v3 92 13 serotypes serotype NNS cord-308950-bl83r4v3 92 14 , , , cord-308950-bl83r4v3 92 15 resulting result VBG cord-308950-bl83r4v3 92 16 in in IN cord-308950-bl83r4v3 92 17 a a DT cord-308950-bl83r4v3 92 18 serious serious JJ cord-308950-bl83r4v3 92 19 problem problem NN cord-308950-bl83r4v3 92 20 in in IN cord-308950-bl83r4v3 92 21 the the DT cord-308950-bl83r4v3 92 22 control control NN cord-308950-bl83r4v3 92 23 and and CC cord-308950-bl83r4v3 92 24 prevention prevention NN cord-308950-bl83r4v3 92 25 of of IN cord-308950-bl83r4v3 92 26 this this DT cord-308950-bl83r4v3 92 27 disease disease NN cord-308950-bl83r4v3 92 28 . . . cord-308950-bl83r4v3 93 1 BHK-21 BHK-21 NNP cord-308950-bl83r4v3 94 1 cells cell NNS cord-308950-bl83r4v3 94 2 are be VBP cord-308950-bl83r4v3 94 3 not not RB cord-308950-bl83r4v3 94 4 naturally naturally RB cord-308950-bl83r4v3 94 5 permissive permissive JJ cord-308950-bl83r4v3 94 6 to to IN cord-308950-bl83r4v3 94 7 this this DT cord-308950-bl83r4v3 94 8 virus virus NN cord-308950-bl83r4v3 94 9 but but CC cord-308950-bl83r4v3 94 10 became become VBD cord-308950-bl83r4v3 94 11 permissive permissive JJ cord-308950-bl83r4v3 94 12 when when WRB cord-308950-bl83r4v3 94 13 transfected transfecte VBN cord-308950-bl83r4v3 94 14 with with IN cord-308950-bl83r4v3 94 15 the the DT cord-308950-bl83r4v3 94 16 fAPN fAPN NNP cord-308950-bl83r4v3 94 17 cDNA cdna NN cord-308950-bl83r4v3 94 18 . . . cord-308950-bl83r4v3 95 1 This this DT cord-308950-bl83r4v3 95 2 result result NN cord-308950-bl83r4v3 95 3 indicates indicate VBZ cord-308950-bl83r4v3 95 4 that that IN cord-308950-bl83r4v3 95 5 the the DT cord-308950-bl83r4v3 95 6 fAPN fapn NN cord-308950-bl83r4v3 95 7 plays play VBZ cord-308950-bl83r4v3 95 8 a a DT cord-308950-bl83r4v3 95 9 role role NN cord-308950-bl83r4v3 95 10 in in IN cord-308950-bl83r4v3 95 11 IBV IBV NNP cord-308950-bl83r4v3 95 12 entry entry NN cord-308950-bl83r4v3 95 13 . . . cord-308950-bl83r4v3 96 1 Previous previous JJ cord-308950-bl83r4v3 96 2 work work NN cord-308950-bl83r4v3 96 3 demonstrated demonstrate VBD cord-308950-bl83r4v3 96 4 that that IN cord-308950-bl83r4v3 96 5 fAPN fAPN NNP cord-308950-bl83r4v3 96 6 serves serve VBZ cord-308950-bl83r4v3 96 7 as as IN cord-308950-bl83r4v3 96 8 the the DT cord-308950-bl83r4v3 96 9 receptor receptor NN cord-308950-bl83r4v3 96 10 for for IN cord-308950-bl83r4v3 96 11 Group Group NNP cord-308950-bl83r4v3 96 12 I -PRON- PRP cord-308950-bl83r4v3 96 13 coronaviruses coronaviruse VBZ cord-308950-bl83r4v3 96 14 infecting infect VBG cord-308950-bl83r4v3 96 15 feline feline JJ cord-308950-bl83r4v3 96 16 cells cell NNS cord-308950-bl83r4v3 96 17 [ [ -LRB- cord-308950-bl83r4v3 96 18 38 38 CD cord-308950-bl83r4v3 96 19 , , , cord-308950-bl83r4v3 96 20 39 39 CD cord-308950-bl83r4v3 96 21 ] ] -RRB- cord-308950-bl83r4v3 96 22 . . . cord-308950-bl83r4v3 97 1 This this DT cord-308950-bl83r4v3 97 2 is be VBZ cord-308950-bl83r4v3 97 3 the the DT cord-308950-bl83r4v3 97 4 first first JJ cord-308950-bl83r4v3 97 5 report report NN cord-308950-bl83r4v3 97 6 indicating indicate VBG cord-308950-bl83r4v3 97 7 that that IN cord-308950-bl83r4v3 97 8 IBV IBV NNP cord-308950-bl83r4v3 97 9 of of IN cord-308950-bl83r4v3 97 10 the the DT cord-308950-bl83r4v3 97 11 Group Group NNP cord-308950-bl83r4v3 97 12 III III NNP cord-308950-bl83r4v3 97 13 coronaviruses coronaviruse NNS cord-308950-bl83r4v3 97 14 also also RB cord-308950-bl83r4v3 97 15 uses use VBZ cord-308950-bl83r4v3 97 16 this this DT cord-308950-bl83r4v3 97 17 receptor receptor NN cord-308950-bl83r4v3 97 18 . . . cord-308950-bl83r4v3 98 1 While while IN cord-308950-bl83r4v3 98 2 the the DT cord-308950-bl83r4v3 98 3 fAPN fapn NN cord-308950-bl83r4v3 98 4 appears appear VBZ cord-308950-bl83r4v3 98 5 to to TO cord-308950-bl83r4v3 98 6 play play VB cord-308950-bl83r4v3 98 7 a a DT cord-308950-bl83r4v3 98 8 role role NN cord-308950-bl83r4v3 98 9 as as IN cord-308950-bl83r4v3 98 10 a a DT cord-308950-bl83r4v3 98 11 receptor receptor NN cord-308950-bl83r4v3 98 12 molecule molecule NN cord-308950-bl83r4v3 98 13 for for IN cord-308950-bl83r4v3 98 14 the the DT cord-308950-bl83r4v3 98 15 Ark Ark NNP cord-308950-bl83r4v3 98 16 / / SYM cord-308950-bl83r4v3 98 17 IBV IBV NNP cord-308950-bl83r4v3 98 18 in in IN cord-308950-bl83r4v3 98 19 feline feline JJ cord-308950-bl83r4v3 98 20 cells cell NNS cord-308950-bl83r4v3 98 21 , , , cord-308950-bl83r4v3 98 22 further further JJ cord-308950-bl83r4v3 98 23 investigation investigation NN cord-308950-bl83r4v3 98 24 is be VBZ cord-308950-bl83r4v3 98 25 needed need VBN cord-308950-bl83r4v3 98 26 to to TO cord-308950-bl83r4v3 98 27 determine determine VB cord-308950-bl83r4v3 98 28 if if IN cord-308950-bl83r4v3 98 29 the the DT cord-308950-bl83r4v3 98 30 chicken chicken NN cord-308950-bl83r4v3 98 31 APN APN NNP cord-308950-bl83r4v3 98 32 plays play VBZ cord-308950-bl83r4v3 98 33 a a DT cord-308950-bl83r4v3 98 34 role role NN cord-308950-bl83r4v3 98 35 in in IN cord-308950-bl83r4v3 98 36 the the DT cord-308950-bl83r4v3 98 37 entry entry NN cord-308950-bl83r4v3 98 38 of of IN cord-308950-bl83r4v3 98 39 the the DT cord-308950-bl83r4v3 98 40 Ark Ark NNP cord-308950-bl83r4v3 98 41 / / SYM cord-308950-bl83r4v3 98 42 IBV IBV NNP cord-308950-bl83r4v3 98 43 in in IN cord-308950-bl83r4v3 98 44 its -PRON- PRP$ cord-308950-bl83r4v3 98 45 natural natural JJ cord-308950-bl83r4v3 98 46 host host NN cord-308950-bl83r4v3 98 47 . . . cord-308950-bl83r4v3 99 1 Role role NN cord-308950-bl83r4v3 99 2 of of IN cord-308950-bl83r4v3 99 3 intestinal intestinal JJ cord-308950-bl83r4v3 99 4 brush brush NN cord-308950-bl83r4v3 99 5 border border NN cord-308950-bl83r4v3 99 6 membrane membrane NN cord-308950-bl83r4v3 99 7 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 99 8 N N NNP cord-308950-bl83r4v3 99 9 in in IN cord-308950-bl83r4v3 99 10 dipeptide dipeptide NN cord-308950-bl83r4v3 99 11 transport transport NN cord-308950-bl83r4v3 99 12 Metalloprotease Metalloprotease NNP cord-308950-bl83r4v3 99 13 activity activity NN cord-308950-bl83r4v3 99 14 of of IN cord-308950-bl83r4v3 99 15 CD13 CD13 NNP cord-308950-bl83r4v3 99 16 / / SYM cord-308950-bl83r4v3 99 17 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 99 18 N N NNP cord-308950-bl83r4v3 99 19 on on IN cord-308950-bl83r4v3 99 20 the the DT cord-308950-bl83r4v3 99 21 surface surface NN cord-308950-bl83r4v3 99 22 of of IN cord-308950-bl83r4v3 99 23 human human JJ cord-308950-bl83r4v3 99 24 myeloid myeloid NN cord-308950-bl83r4v3 99 25 cells cell NNS cord-308950-bl83r4v3 100 1 A a DT cord-308950-bl83r4v3 100 2 clustering clustering NN cord-308950-bl83r4v3 100 3 of of IN cord-308950-bl83r4v3 100 4 RNA RNA NNP cord-308950-bl83r4v3 100 5 recombination recombination NN cord-308950-bl83r4v3 100 6 sites site NNS cord-308950-bl83r4v3 100 7 adjacent adjacent JJ cord-308950-bl83r4v3 100 8 to to IN cord-308950-bl83r4v3 100 9 a a DT cord-308950-bl83r4v3 100 10 hypervariable hypervariable JJ cord-308950-bl83r4v3 100 11 region region NN cord-308950-bl83r4v3 100 12 of of IN cord-308950-bl83r4v3 100 13 the the DT cord-308950-bl83r4v3 100 14 peplomer peplomer JJ cord-308950-bl83r4v3 100 15 gene gene NN cord-308950-bl83r4v3 100 16 of of IN cord-308950-bl83r4v3 100 17 murine murine JJ cord-308950-bl83r4v3 100 18 coronavirus coronavirus NN cord-308950-bl83r4v3 100 19 Experimental experimental JJ cord-308950-bl83r4v3 100 20 inoculation inoculation NN cord-308950-bl83r4v3 100 21 of of IN cord-308950-bl83r4v3 100 22 cats cat NNS cord-308950-bl83r4v3 100 23 with with IN cord-308950-bl83r4v3 100 24 canine canine JJ cord-308950-bl83r4v3 100 25 coronavirus coronavirus NN cord-308950-bl83r4v3 100 26 and and CC cord-308950-bl83r4v3 100 27 subsequent subsequent JJ cord-308950-bl83r4v3 100 28 challenge challenge NN cord-308950-bl83r4v3 100 29 with with IN cord-308950-bl83r4v3 100 30 feline feline JJ cord-308950-bl83r4v3 100 31 infectious infectious JJ cord-308950-bl83r4v3 100 32 peritonitis peritonitis NN cord-308950-bl83r4v3 100 33 virus virus NN cord-308950-bl83r4v3 100 34 Experimental experimental JJ cord-308950-bl83r4v3 100 35 inoculation inoculation NN cord-308950-bl83r4v3 100 36 of of IN cord-308950-bl83r4v3 100 37 cats cat NNS cord-308950-bl83r4v3 100 38 with with IN cord-308950-bl83r4v3 100 39 human human JJ cord-308950-bl83r4v3 100 40 coronavirus coronavirus NN cord-308950-bl83r4v3 100 41 229E 229e CD cord-308950-bl83r4v3 100 42 and and CC cord-308950-bl83r4v3 100 43 subsequent subsequent JJ cord-308950-bl83r4v3 100 44 challenge challenge NN cord-308950-bl83r4v3 100 45 with with IN cord-308950-bl83r4v3 100 46 feline feline JJ cord-308950-bl83r4v3 100 47 infectious infectious JJ cord-308950-bl83r4v3 100 48 peritonitis peritonitis NN cord-308950-bl83r4v3 100 49 virus virus NN cord-308950-bl83r4v3 100 50 Localization localization NN cord-308950-bl83r4v3 100 51 of of IN cord-308950-bl83r4v3 100 52 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 100 53 N n NN cord-308950-bl83r4v3 100 54 and and CC cord-308950-bl83r4v3 100 55 dipeptidyl dipeptidyl JJ cord-308950-bl83r4v3 100 56 peptidase peptidase NN cord-308950-bl83r4v3 100 57 IV IV NNP cord-308950-bl83r4v3 100 58 in in IN cord-308950-bl83r4v3 100 59 pig pig NN cord-308950-bl83r4v3 100 60 striatum striatum NN cord-308950-bl83r4v3 100 61 and and CC cord-308950-bl83r4v3 100 62 in in IN cord-308950-bl83r4v3 100 63 neuronal neuronal JJ cord-308950-bl83r4v3 100 64 and and CC cord-308950-bl83r4v3 100 65 glial glial JJ cord-308950-bl83r4v3 100 66 cell cell NN cord-308950-bl83r4v3 100 67 cultures culture NNS cord-308950-bl83r4v3 101 1 Interspecies interspecies JJ cord-308950-bl83r4v3 101 2 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 101 3 - - HYPH cord-308950-bl83r4v3 101 4 N n CD cord-308950-bl83r4v3 101 5 chimeras chimera NNS cord-308950-bl83r4v3 101 6 reveal reveal VBP cord-308950-bl83r4v3 101 7 species species NN cord-308950-bl83r4v3 101 8 - - HYPH cord-308950-bl83r4v3 101 9 specific specific JJ cord-308950-bl83r4v3 101 10 receptor receptor NN cord-308950-bl83r4v3 101 11 recognition recognition NN cord-308950-bl83r4v3 101 12 by by IN cord-308950-bl83r4v3 101 13 canine canine JJ cord-308950-bl83r4v3 101 14 coronavirus coronavirus NN cord-308950-bl83r4v3 101 15 , , , cord-308950-bl83r4v3 101 16 feline feline JJ cord-308950-bl83r4v3 101 17 infectious infectious JJ cord-308950-bl83r4v3 101 18 peritonitis peritonitis NN cord-308950-bl83r4v3 101 19 virus virus NN cord-308950-bl83r4v3 101 20 , , , cord-308950-bl83r4v3 101 21 and and CC cord-308950-bl83r4v3 101 22 transmissible transmissible JJ cord-308950-bl83r4v3 101 23 gastroenteritis gastroenteritis NN cord-308950-bl83r4v3 101 24 virus virus NN cord-308950-bl83r4v3 101 25 Biosynthesis Biosynthesis NNP cord-308950-bl83r4v3 101 26 of of IN cord-308950-bl83r4v3 101 27 intestinal intestinal JJ cord-308950-bl83r4v3 101 28 microvillar microvillar JJ cord-308950-bl83r4v3 101 29 proteins protein NNS cord-308950-bl83r4v3 101 30 . . . cord-308950-bl83r4v3 102 1 Forskolin forskolin NN cord-308950-bl83r4v3 102 2 reduces reduce VBZ cord-308950-bl83r4v3 102 3 surface surface NN cord-308950-bl83r4v3 102 4 expression expression NN cord-308950-bl83r4v3 102 5 of of IN cord-308950-bl83r4v3 102 6 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 103 1 Aminopeptidase Aminopeptidase NNP cord-308950-bl83r4v3 103 2 N N NNP cord-308950-bl83r4v3 103 3 is be VBZ cord-308950-bl83r4v3 103 4 a a DT cord-308950-bl83r4v3 103 5 major major JJ cord-308950-bl83r4v3 103 6 receptor receptor NN cord-308950-bl83r4v3 103 7 for for IN cord-308950-bl83r4v3 103 8 the the DT cord-308950-bl83r4v3 103 9 entero entero NNP cord-308950-bl83r4v3 103 10 - - HYPH cord-308950-bl83r4v3 103 11 pathogenic pathogenic JJ cord-308950-bl83r4v3 103 12 coronavirus coronavirus NN cord-308950-bl83r4v3 104 1 TGEV TGEV NNP cord-308950-bl83r4v3 104 2 Further further JJ cord-308950-bl83r4v3 104 3 characterization characterization NN cord-308950-bl83r4v3 104 4 of of IN cord-308950-bl83r4v3 104 5 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 104 6 - - HYPH cord-308950-bl83r4v3 104 7 N N NNP cord-308950-bl83r4v3 104 8 as as IN cord-308950-bl83r4v3 104 9 a a DT cord-308950-bl83r4v3 104 10 receptor receptor NN cord-308950-bl83r4v3 104 11 for for IN cord-308950-bl83r4v3 104 12 coronaviruses coronaviruse NNS cord-308950-bl83r4v3 105 1 Determinants determinant NNS cord-308950-bl83r4v3 105 2 essential essential JJ cord-308950-bl83r4v3 105 3 for for IN cord-308950-bl83r4v3 105 4 the the DT cord-308950-bl83r4v3 105 5 transmissible transmissible JJ cord-308950-bl83r4v3 105 6 gastroenteritis gastroenteritis JJ cord-308950-bl83r4v3 105 7 virus virus NN cord-308950-bl83r4v3 105 8 - - HYPH cord-308950-bl83r4v3 105 9 receptor receptor NN cord-308950-bl83r4v3 105 10 interaction interaction NN cord-308950-bl83r4v3 105 11 reside reside VBP cord-308950-bl83r4v3 105 12 within within IN cord-308950-bl83r4v3 105 13 a a DT cord-308950-bl83r4v3 105 14 domain domain NN cord-308950-bl83r4v3 105 15 of of IN cord-308950-bl83r4v3 105 16 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 105 17 - - HYPH cord-308950-bl83r4v3 105 18 N n NN cord-308950-bl83r4v3 105 19 that that WDT cord-308950-bl83r4v3 105 20 is be VBZ cord-308950-bl83r4v3 105 21 distinct distinct JJ cord-308950-bl83r4v3 105 22 from from IN cord-308950-bl83r4v3 105 23 the the DT cord-308950-bl83r4v3 105 24 enzymatic enzymatic JJ cord-308950-bl83r4v3 105 25 site site NN cord-308950-bl83r4v3 106 1 Aminopeptidase Aminopeptidase NNP cord-308950-bl83r4v3 106 2 N N NNP cord-308950-bl83r4v3 106 3 is be VBZ cord-308950-bl83r4v3 106 4 a a DT cord-308950-bl83r4v3 106 5 marker marker NN cord-308950-bl83r4v3 106 6 for for IN cord-308950-bl83r4v3 106 7 the the DT cord-308950-bl83r4v3 106 8 apical apical JJ cord-308950-bl83r4v3 106 9 pole pole NN cord-308950-bl83r4v3 106 10 of of IN cord-308950-bl83r4v3 106 11 porcine porcine JJ cord-308950-bl83r4v3 106 12 thyroid thyroid JJ cord-308950-bl83r4v3 106 13 epithelial epithelial JJ cord-308950-bl83r4v3 106 14 cells cell NNS cord-308950-bl83r4v3 106 15 in in IN cord-308950-bl83r4v3 106 16 vivo vivo NN cord-308950-bl83r4v3 106 17 and and CC cord-308950-bl83r4v3 106 18 in in IN cord-308950-bl83r4v3 106 19 culture culture NN cord-308950-bl83r4v3 106 20 Evidence evidence NN cord-308950-bl83r4v3 106 21 for for IN cord-308950-bl83r4v3 106 22 variable variable JJ cord-308950-bl83r4v3 106 23 rates rate NNS cord-308950-bl83r4v3 106 24 of of IN cord-308950-bl83r4v3 106 25 recombination recombination NN cord-308950-bl83r4v3 106 26 in in IN cord-308950-bl83r4v3 106 27 the the DT cord-308950-bl83r4v3 106 28 MHV MHV NNP cord-308950-bl83r4v3 106 29 genome genome NN cord-308950-bl83r4v3 106 30 Chicken Chicken NNP cord-308950-bl83r4v3 106 31 intestinal intestinal JJ cord-308950-bl83r4v3 106 32 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 106 33 : : : cord-308950-bl83r4v3 106 34 Partial partial JJ cord-308950-bl83r4v3 106 35 sequence sequence NN cord-308950-bl83r4v3 106 36 of of IN cord-308950-bl83r4v3 106 37 the the DT cord-308950-bl83r4v3 106 38 gene gene NN cord-308950-bl83r4v3 106 39 , , , cord-308950-bl83r4v3 106 40 expression expression NN cord-308950-bl83r4v3 106 41 and and CC cord-308950-bl83r4v3 106 42 activity activity NN cord-308950-bl83r4v3 106 43 Organelles organelle NNS cord-308950-bl83r4v3 106 44 involved involve VBN cord-308950-bl83r4v3 106 45 in in IN cord-308950-bl83r4v3 106 46 the the DT cord-308950-bl83r4v3 106 47 intracellular intracellular JJ cord-308950-bl83r4v3 106 48 transport transport NN cord-308950-bl83r4v3 106 49 of of IN cord-308950-bl83r4v3 106 50 newly newly RB cord-308950-bl83r4v3 106 51 synthesized synthesize VBN cord-308950-bl83r4v3 106 52 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 106 53 N n NN cord-308950-bl83r4v3 106 54 and and CC cord-308950-bl83r4v3 106 55 their -PRON- PRP$ cord-308950-bl83r4v3 106 56 acidity acidity NN cord-308950-bl83r4v3 106 57 Characterization characterization NN cord-308950-bl83r4v3 106 58 of of IN cord-308950-bl83r4v3 106 59 determinants determinant NNS cord-308950-bl83r4v3 106 60 involved involve VBN cord-308950-bl83r4v3 106 61 in in IN cord-308950-bl83r4v3 106 62 the the DT cord-308950-bl83r4v3 106 63 feline feline JJ cord-308950-bl83r4v3 106 64 infectious infectious JJ cord-308950-bl83r4v3 106 65 peritonitis peritonitis NN cord-308950-bl83r4v3 106 66 virus virus NN cord-308950-bl83r4v3 106 67 receptor receptor NN cord-308950-bl83r4v3 106 68 function function NN cord-308950-bl83r4v3 106 69 of of IN cord-308950-bl83r4v3 106 70 feline feline JJ cord-308950-bl83r4v3 106 71 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 106 72 N n CD cord-308950-bl83r4v3 106 73 Feline feline JJ cord-308950-bl83r4v3 106 74 coronavirus coronavirus NN cord-308950-bl83r4v3 106 75 type type NN cord-308950-bl83r4v3 106 76 II ii CD cord-308950-bl83r4v3 106 77 strains strain VBZ cord-308950-bl83r4v3 106 78 79 79 CD cord-308950-bl83r4v3 106 79 - - HYPH cord-308950-bl83r4v3 106 80 1683 1683 CD cord-308950-bl83r4v3 106 81 originate originate VBP cord-308950-bl83r4v3 106 82 from from IN cord-308950-bl83r4v3 106 83 a a DT cord-308950-bl83r4v3 106 84 double double JJ cord-308950-bl83r4v3 106 85 recombination recombination NN cord-308950-bl83r4v3 106 86 between between IN cord-308950-bl83r4v3 106 87 feline feline JJ cord-308950-bl83r4v3 106 88 coronavirus coronavirus NN cord-308950-bl83r4v3 106 89 type type NN cord-308950-bl83r4v3 106 90 I -PRON- PRP cord-308950-bl83r4v3 106 91 and and CC cord-308950-bl83r4v3 106 92 canine canine JJ cord-308950-bl83r4v3 106 93 coronavirus coronavirus NN cord-308950-bl83r4v3 106 94 Production production NN cord-308950-bl83r4v3 106 95 and and CC cord-308950-bl83r4v3 106 96 immunogenicity immunogenicity NN cord-308950-bl83r4v3 106 97 of of IN cord-308950-bl83r4v3 106 98 multiple multiple JJ cord-308950-bl83r4v3 106 99 antigenic antigenic JJ cord-308950-bl83r4v3 106 100 peptide peptide NN cord-308950-bl83r4v3 106 101 ( ( -LRB- cord-308950-bl83r4v3 106 102 MAP MAP NNP cord-308950-bl83r4v3 106 103 ) ) -RRB- cord-308950-bl83r4v3 106 104 constructs construct NNS cord-308950-bl83r4v3 106 105 derived derive VBN cord-308950-bl83r4v3 106 106 from from IN cord-308950-bl83r4v3 106 107 the the DT cord-308950-bl83r4v3 106 108 S1 S1 NNP cord-308950-bl83r4v3 106 109 glycoprotein glycoprotein NN cord-308950-bl83r4v3 106 110 of of IN cord-308950-bl83r4v3 106 111 infectious infectious JJ cord-308950-bl83r4v3 106 112 bronchitis bronchitis NN cord-308950-bl83r4v3 106 113 virus virus NN cord-308950-bl83r4v3 106 114 ( ( -LRB- cord-308950-bl83r4v3 106 115 IBV IBV NNP cord-308950-bl83r4v3 106 116 ) ) -RRB- cord-308950-bl83r4v3 107 1 A a DT cord-308950-bl83r4v3 107 2 novel novel JJ cord-308950-bl83r4v3 107 3 variant variant NN cord-308950-bl83r4v3 107 4 of of IN cord-308950-bl83r4v3 107 5 infectious infectious JJ cord-308950-bl83r4v3 107 6 bronchitis bronchitis NN cord-308950-bl83r4v3 107 7 virus virus NN cord-308950-bl83r4v3 107 8 resulting result VBG cord-308950-bl83r4v3 107 9 from from IN cord-308950-bl83r4v3 107 10 recombination recombination NN cord-308950-bl83r4v3 107 11 among among IN cord-308950-bl83r4v3 107 12 three three CD cord-308950-bl83r4v3 107 13 different different JJ cord-308950-bl83r4v3 107 14 strains strain NNS cord-308950-bl83r4v3 107 15 Antigenic antigenic JJ cord-308950-bl83r4v3 107 16 domains domain NNS cord-308950-bl83r4v3 107 17 on on IN cord-308950-bl83r4v3 107 18 the the DT cord-308950-bl83r4v3 107 19 peplomer peplomer JJ cord-308950-bl83r4v3 107 20 protein protein NN cord-308950-bl83r4v3 107 21 of of IN cord-308950-bl83r4v3 107 22 avian avian JJ cord-308950-bl83r4v3 107 23 infectious infectious JJ cord-308950-bl83r4v3 107 24 bronchitis bronchitis NN cord-308950-bl83r4v3 107 25 virus virus NN cord-308950-bl83r4v3 107 26 : : : cord-308950-bl83r4v3 107 27 correlation correlation NN cord-308950-bl83r4v3 107 28 with with IN cord-308950-bl83r4v3 107 29 biological biological JJ cord-308950-bl83r4v3 107 30 functions function NNS cord-308950-bl83r4v3 107 31 Characterization characterization NN cord-308950-bl83r4v3 107 32 of of IN cord-308950-bl83r4v3 107 33 functional functional JJ cord-308950-bl83r4v3 107 34 domains domain NNS cord-308950-bl83r4v3 107 35 in in IN cord-308950-bl83r4v3 107 36 the the DT cord-308950-bl83r4v3 107 37 human human JJ cord-308950-bl83r4v3 107 38 coronavirus coronavirus NN cord-308950-bl83r4v3 108 1 HCV HCV NNP cord-308950-bl83r4v3 108 2 229E 229E NNP cord-308950-bl83r4v3 108 3 receptor receptor NN cord-308950-bl83r4v3 108 4 Identification identification NN cord-308950-bl83r4v3 108 5 of of IN cord-308950-bl83r4v3 108 6 residues residue NNS cord-308950-bl83r4v3 108 7 critical critical JJ cord-308950-bl83r4v3 108 8 for for IN cord-308950-bl83r4v3 108 9 the the DT cord-308950-bl83r4v3 108 10 human human JJ cord-308950-bl83r4v3 108 11 coronavirus coronavirus NN cord-308950-bl83r4v3 108 12 229E 229e CD cord-308950-bl83r4v3 108 13 receptor receptor NN cord-308950-bl83r4v3 108 14 function function NN cord-308950-bl83r4v3 108 15 of of IN cord-308950-bl83r4v3 108 16 human human JJ cord-308950-bl83r4v3 108 17 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 108 18 N N NNP cord-308950-bl83r4v3 108 19 Molecular Molecular NNP cord-308950-bl83r4v3 108 20 analysis analysis NN cord-308950-bl83r4v3 108 21 of of IN cord-308950-bl83r4v3 108 22 the the DT cord-308950-bl83r4v3 108 23 coronavirus coronavirus NN cord-308950-bl83r4v3 108 24 - - HYPH cord-308950-bl83r4v3 108 25 receptor receptor NN cord-308950-bl83r4v3 108 26 function function NN cord-308950-bl83r4v3 108 27 of of IN cord-308950-bl83r4v3 108 28 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 108 29 N N NNP cord-308950-bl83r4v3 108 30 First First NNP cord-308950-bl83r4v3 108 31 experimental experimental JJ cord-308950-bl83r4v3 108 32 evidence evidence NN cord-308950-bl83r4v3 108 33 of of IN cord-308950-bl83r4v3 108 34 recombination recombination NN cord-308950-bl83r4v3 108 35 in in IN cord-308950-bl83r4v3 108 36 infectious infectious JJ cord-308950-bl83r4v3 108 37 bronchitis bronchitis NN cord-308950-bl83r4v3 108 38 virus virus NN cord-308950-bl83r4v3 108 39 . . . cord-308950-bl83r4v3 109 1 Recombination recombination NN cord-308950-bl83r4v3 109 2 in in IN cord-308950-bl83r4v3 109 3 IBV IBV NNP cord-308950-bl83r4v3 109 4 Experimental experimental JJ cord-308950-bl83r4v3 109 5 evidence evidence NN cord-308950-bl83r4v3 109 6 of of IN cord-308950-bl83r4v3 109 7 recombination recombination NN cord-308950-bl83r4v3 109 8 in in IN cord-308950-bl83r4v3 109 9 coronavirus coronavirus NN cord-308950-bl83r4v3 109 10 infectious infectious JJ cord-308950-bl83r4v3 109 11 bronchitis bronchitis NN cord-308950-bl83r4v3 109 12 virus virus NN cord-308950-bl83r4v3 109 13 Aminopeptidase Aminopeptidase NNP cord-308950-bl83r4v3 109 14 N N NNP cord-308950-bl83r4v3 109 15 ( ( -LRB- cord-308950-bl83r4v3 109 16 CD13 CD13 NNP cord-308950-bl83r4v3 109 17 , , , cord-308950-bl83r4v3 109 18 EC EC NNP cord-308950-bl83r4v3 109 19 3.3.4.11.2 3.3.4.11.2 CD cord-308950-bl83r4v3 109 20 ) ) -RRB- cord-308950-bl83r4v3 109 21 occurs occur VBZ cord-308950-bl83r4v3 109 22 on on IN cord-308950-bl83r4v3 109 23 the the DT cord-308950-bl83r4v3 109 24 surface surface NN cord-308950-bl83r4v3 109 25 of of IN cord-308950-bl83r4v3 109 26 resting rest VBG cord-308950-bl83r4v3 109 27 and and CC cord-308950-bl83r4v3 109 28 concanavalin concanavalin NNP cord-308950-bl83r4v3 109 29 A A NNP cord-308950-bl83r4v3 109 30 - - HYPH cord-308950-bl83r4v3 109 31 stimulated stimulate VBN cord-308950-bl83r4v3 109 32 lymphocytes lymphocyte NNS cord-308950-bl83r4v3 110 1 Sequence sequence NN cord-308950-bl83r4v3 110 2 evidence evidence NN cord-308950-bl83r4v3 110 3 for for IN cord-308950-bl83r4v3 110 4 RNA rna NN cord-308950-bl83r4v3 110 5 recombination recombination NN cord-308950-bl83r4v3 110 6 in in IN cord-308950-bl83r4v3 110 7 field field NN cord-308950-bl83r4v3 110 8 isolates isolate NNS cord-308950-bl83r4v3 110 9 of of IN cord-308950-bl83r4v3 110 10 avian avian JJ cord-308950-bl83r4v3 110 11 coronavirus coronavirus NN cord-308950-bl83r4v3 110 12 infectious infectious JJ cord-308950-bl83r4v3 110 13 bronchitis bronchitis NN cord-308950-bl83r4v3 110 14 virus virus NN cord-308950-bl83r4v3 110 15 Molecular molecular JJ cord-308950-bl83r4v3 110 16 cloning clone VBG cord-308950-bl83r4v3 110 17 and and CC cord-308950-bl83r4v3 110 18 sequence sequence NN cord-308950-bl83r4v3 110 19 comparison comparison NN cord-308950-bl83r4v3 110 20 of of IN cord-308950-bl83r4v3 110 21 the the DT cord-308950-bl83r4v3 110 22 S1 S1 NNP cord-308950-bl83r4v3 110 23 glycoprotein glycoprotein NN cord-308950-bl83r4v3 110 24 of of IN cord-308950-bl83r4v3 110 25 the the DT cord-308950-bl83r4v3 110 26 Gray Gray NNP cord-308950-bl83r4v3 110 27 and and CC cord-308950-bl83r4v3 110 28 JMK JMK NNP cord-308950-bl83r4v3 110 29 strains strain NNS cord-308950-bl83r4v3 110 30 of of IN cord-308950-bl83r4v3 110 31 avian avian JJ cord-308950-bl83r4v3 110 32 infectious infectious JJ cord-308950-bl83r4v3 110 33 bronchitis bronchitis NN cord-308950-bl83r4v3 110 34 virus virus NN cord-308950-bl83r4v3 110 35 Evidence evidence NN cord-308950-bl83r4v3 110 36 of of IN cord-308950-bl83r4v3 110 37 genetic genetic JJ cord-308950-bl83r4v3 110 38 diversity diversity NN cord-308950-bl83r4v3 110 39 generated generate VBN cord-308950-bl83r4v3 110 40 by by IN cord-308950-bl83r4v3 110 41 recombination recombination NN cord-308950-bl83r4v3 110 42 among among IN cord-308950-bl83r4v3 110 43 avian avian JJ cord-308950-bl83r4v3 110 44 coronavirus coronavirus NN cord-308950-bl83r4v3 110 45 IBV IBV NNP cord-308950-bl83r4v3 110 46 Expression Expression NNP cord-308950-bl83r4v3 110 47 of of IN cord-308950-bl83r4v3 110 48 the the DT cord-308950-bl83r4v3 110 49 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 110 50 N N NNP cord-308950-bl83r4v3 110 51 ( ( -LRB- cord-308950-bl83r4v3 110 52 CD13 CD13 NNP cord-308950-bl83r4v3 110 53 ) ) -RRB- cord-308950-bl83r4v3 110 54 gene gene NN cord-308950-bl83r4v3 110 55 in in IN cord-308950-bl83r4v3 110 56 the the DT cord-308950-bl83r4v3 110 57 human human JJ cord-308950-bl83r4v3 110 58 T T NNP cord-308950-bl83r4v3 110 59 cell cell NN cord-308950-bl83r4v3 110 60 lines line NNS cord-308950-bl83r4v3 110 61 HuT78 HuT78 NNP cord-308950-bl83r4v3 110 62 and and CC cord-308950-bl83r4v3 110 63 H9 H9 NNP cord-308950-bl83r4v3 110 64 Human Human NNP cord-308950-bl83r4v3 110 65 myeloid myeloid NN cord-308950-bl83r4v3 110 66 plasma plasma NN cord-308950-bl83r4v3 110 67 membrane membrane NN cord-308950-bl83r4v3 110 68 glycoprotein glycoprotein NN cord-308950-bl83r4v3 110 69 CD13 CD13 NNP cord-308950-bl83r4v3 110 70 ( ( -LRB- cord-308950-bl83r4v3 110 71 gp150 gp150 NNP cord-308950-bl83r4v3 110 72 ) ) -RRB- cord-308950-bl83r4v3 110 73 is be VBZ cord-308950-bl83r4v3 110 74 identical identical JJ cord-308950-bl83r4v3 110 75 to to IN cord-308950-bl83r4v3 110 76 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 110 77 N n CD cord-308950-bl83r4v3 110 78 Isolation isolation NN cord-308950-bl83r4v3 110 79 and and CC cord-308950-bl83r4v3 110 80 characterization characterization NN cord-308950-bl83r4v3 110 81 of of IN cord-308950-bl83r4v3 110 82 cDNA cdna NN cord-308950-bl83r4v3 110 83 encoding encode VBG cord-308950-bl83r4v3 110 84 chicken chicken NN cord-308950-bl83r4v3 110 85 egg egg NN cord-308950-bl83r4v3 110 86 yolk yolk NN cord-308950-bl83r4v3 110 87 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 110 88 Ey Ey NNP cord-308950-bl83r4v3 110 89 Structure Structure NNP cord-308950-bl83r4v3 110 90 and and CC cord-308950-bl83r4v3 110 91 expression expression NN cord-308950-bl83r4v3 110 92 of of IN cord-308950-bl83r4v3 110 93 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 111 1 N N NNP cord-308950-bl83r4v3 112 1 An an DT cord-308950-bl83r4v3 112 2 enhancer enhancer NN cord-308950-bl83r4v3 112 3 with with IN cord-308950-bl83r4v3 112 4 cell cell NN cord-308950-bl83r4v3 112 5 - - HYPH cord-308950-bl83r4v3 112 6 type type NN cord-308950-bl83r4v3 112 7 dependent dependent JJ cord-308950-bl83r4v3 112 8 activity activity NN cord-308950-bl83r4v3 112 9 is be VBZ cord-308950-bl83r4v3 112 10 located locate VBN cord-308950-bl83r4v3 112 11 between between IN cord-308950-bl83r4v3 112 12 the the DT cord-308950-bl83r4v3 112 13 myeloid myeloid JJ cord-308950-bl83r4v3 112 14 and and CC cord-308950-bl83r4v3 112 15 epithelial epithelial JJ cord-308950-bl83r4v3 112 16 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 112 17 N N NNP cord-308950-bl83r4v3 112 18 ( ( -LRB- cord-308950-bl83r4v3 112 19 CD CD NNP cord-308950-bl83r4v3 112 20 13 13 CD cord-308950-bl83r4v3 112 21 ) ) -RRB- cord-308950-bl83r4v3 112 22 promoters promoter NNS cord-308950-bl83r4v3 112 23 Induction Induction NNP cord-308950-bl83r4v3 112 24 of of IN cord-308950-bl83r4v3 112 25 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 113 1 N N NNP cord-308950-bl83r4v3 113 2 / / SYM cord-308950-bl83r4v3 113 3 CD13 CD13 NNP cord-308950-bl83r4v3 113 4 on on IN cord-308950-bl83r4v3 113 5 human human JJ cord-308950-bl83r4v3 113 6 lymphocytes lymphocyte NNS cord-308950-bl83r4v3 113 7 after after IN cord-308950-bl83r4v3 113 8 adhesion adhesion NN cord-308950-bl83r4v3 113 9 to to IN cord-308950-bl83r4v3 113 10 fibroblast fibroblast NN cord-308950-bl83r4v3 113 11 - - HYPH cord-308950-bl83r4v3 113 12 like like IN cord-308950-bl83r4v3 113 13 synoviocytes synoviocyte NNS cord-308950-bl83r4v3 113 14 , , , cord-308950-bl83r4v3 113 15 endothelial endothelial JJ cord-308950-bl83r4v3 113 16 cells cell NNS cord-308950-bl83r4v3 113 17 , , , cord-308950-bl83r4v3 113 18 epithelial epithelial JJ cord-308950-bl83r4v3 113 19 cells cell NNS cord-308950-bl83r4v3 113 20 , , , cord-308950-bl83r4v3 113 21 and and CC cord-308950-bl83r4v3 113 22 monocytes monocyte NNS cord-308950-bl83r4v3 113 23 / / SYM cord-308950-bl83r4v3 113 24 macrophages macrophage NNS cord-308950-bl83r4v3 114 1 Look look VB cord-308950-bl83r4v3 114 2 AT at IN cord-308950-bl83r4v3 114 3 ( ( -LRB- cord-308950-bl83r4v3 114 4 1991 1991 CD cord-308950-bl83r4v3 114 5 ) ) -RRB- cord-308950-bl83r4v3 115 1 Separate separate JJ cord-308950-bl83r4v3 115 2 promoters promoter NNS cord-308950-bl83r4v3 115 3 control control VBP cord-308950-bl83r4v3 115 4 transcription transcription NN cord-308950-bl83r4v3 115 5 of of IN cord-308950-bl83r4v3 115 6 the the DT cord-308950-bl83r4v3 115 7 human human JJ cord-308950-bl83r4v3 115 8 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 115 9 N n CD cord-308950-bl83r4v3 115 10 gene gene NN cord-308950-bl83r4v3 115 11 in in IN cord-308950-bl83r4v3 115 12 myeloid myeloid JJ cord-308950-bl83r4v3 115 13 and and CC cord-308950-bl83r4v3 115 14 intestinal intestinal JJ cord-308950-bl83r4v3 115 15 epithelial epithelial JJ cord-308950-bl83r4v3 115 16 cells cell NNS cord-308950-bl83r4v3 115 17 Induction induction NN cord-308950-bl83r4v3 115 18 of of IN cord-308950-bl83r4v3 115 19 protective protective JJ cord-308950-bl83r4v3 115 20 immunity immunity NN cord-308950-bl83r4v3 115 21 in in IN cord-308950-bl83r4v3 115 22 chickens chicken NNS cord-308950-bl83r4v3 115 23 vaccinated vaccinate VBN cord-308950-bl83r4v3 115 24 with with IN cord-308950-bl83r4v3 115 25 infectious infectious JJ cord-308950-bl83r4v3 115 26 bronchitis bronchitis NN cord-308950-bl83r4v3 115 27 virus virus NN cord-308950-bl83r4v3 115 28 S1 S1 NNP cord-308950-bl83r4v3 115 29 glycoprotein glycoprotein NN cord-308950-bl83r4v3 115 30 expressed express VBD cord-308950-bl83r4v3 115 31 in in IN cord-308950-bl83r4v3 115 32 a a DT cord-308950-bl83r4v3 115 33 recombinant recombinant JJ cord-308950-bl83r4v3 115 34 baculovirus baculovirus NNP cord-308950-bl83r4v3 115 35 Feline feline JJ cord-308950-bl83r4v3 115 36 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 115 37 N N NNP cord-308950-bl83r4v3 115 38 is be VBZ cord-308950-bl83r4v3 115 39 a a DT cord-308950-bl83r4v3 115 40 receptor receptor NN cord-308950-bl83r4v3 115 41 for for IN cord-308950-bl83r4v3 115 42 all all DT cord-308950-bl83r4v3 115 43 group group NN cord-308950-bl83r4v3 115 44 I -PRON- PRP cord-308950-bl83r4v3 115 45 coronaviruses coronaviruse VBZ cord-308950-bl83r4v3 115 46 Feline feline JJ cord-308950-bl83r4v3 115 47 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 115 48 N n CD cord-308950-bl83r4v3 115 49 serves serve VBZ cord-308950-bl83r4v3 115 50 as as IN cord-308950-bl83r4v3 115 51 a a DT cord-308950-bl83r4v3 115 52 receptor receptor NN cord-308950-bl83r4v3 115 53 for for IN cord-308950-bl83r4v3 115 54 feline feline JJ cord-308950-bl83r4v3 115 55 , , , cord-308950-bl83r4v3 115 56 canine canine JJ cord-308950-bl83r4v3 115 57 , , , cord-308950-bl83r4v3 115 58 porcine porcine JJ cord-308950-bl83r4v3 115 59 , , , cord-308950-bl83r4v3 115 60 and and CC cord-308950-bl83r4v3 115 61 human human JJ cord-308950-bl83r4v3 115 62 coronaviruses coronaviruse NNS cord-308950-bl83r4v3 115 63 in in IN cord-308950-bl83r4v3 115 64 serogroup serogroup NNP cord-308950-bl83r4v3 116 1 I -PRON- PRP cord-308950-bl83r4v3 116 2 Isolation isolation NN cord-308950-bl83r4v3 116 3 and and CC cord-308950-bl83r4v3 116 4 identification identification NN cord-308950-bl83r4v3 116 5 of of IN cord-308950-bl83r4v3 116 6 avian avian JJ cord-308950-bl83r4v3 116 7 pathogens pathogen NNS cord-308950-bl83r4v3 116 8 , , , cord-308950-bl83r4v3 116 9 4th 4th JJ cord-308950-bl83r4v3 116 10 edn edn NNP cord-308950-bl83r4v3 116 11 fAPN fapn NN cord-308950-bl83r4v3 116 12 as as IN cord-308950-bl83r4v3 116 13 a a DT cord-308950-bl83r4v3 116 14 receptor receptor NN cord-308950-bl83r4v3 116 15 for for IN cord-308950-bl83r4v3 116 16 infectious infectious JJ cord-308950-bl83r4v3 116 17 bronchitis bronchitis NN cord-308950-bl83r4v3 116 18 virus virus NN cord-308950-bl83r4v3 116 19 Evidence evidence NN cord-308950-bl83r4v3 116 20 of of IN cord-308950-bl83r4v3 116 21 natural natural JJ cord-308950-bl83r4v3 116 22 recombination recombination NN cord-308950-bl83r4v3 116 23 within within IN cord-308950-bl83r4v3 116 24 the the DT cord-308950-bl83r4v3 116 25 S1 S1 NNP cord-308950-bl83r4v3 116 26 gene gene NN cord-308950-bl83r4v3 116 27 of of IN cord-308950-bl83r4v3 116 28 infectious infectious JJ cord-308950-bl83r4v3 116 29 bronchitis bronchitis NN cord-308950-bl83r4v3 116 30 virus virus NN cord-308950-bl83r4v3 116 31 Experimental experimental JJ cord-308950-bl83r4v3 116 32 confirmation confirmation NN cord-308950-bl83r4v3 116 33 of of IN cord-308950-bl83r4v3 116 34 recombination recombination NN cord-308950-bl83r4v3 116 35 upstream upstream RB cord-308950-bl83r4v3 116 36 of of IN cord-308950-bl83r4v3 116 37 the the DT cord-308950-bl83r4v3 116 38 S1 S1 NNP cord-308950-bl83r4v3 116 39 hypervariable hypervariable NN cord-308950-bl83r4v3 116 40 region region NN cord-308950-bl83r4v3 116 41 of of IN cord-308950-bl83r4v3 116 42 infectious infectious JJ cord-308950-bl83r4v3 116 43 bronchitis bronchitis NN cord-308950-bl83r4v3 116 44 virus virus NN cord-308950-bl83r4v3 116 45 Human Human NNP cord-308950-bl83r4v3 116 46 aminopeptidase aminopeptidase NN cord-308950-bl83r4v3 117 1 N N NNP cord-308950-bl83r4v3 117 2 is be VBZ cord-308950-bl83r4v3 117 3 a a DT cord-308950-bl83r4v3 117 4 receptor receptor NN cord-308950-bl83r4v3 117 5 for for IN cord-308950-bl83r4v3 117 6 human human JJ cord-308950-bl83r4v3 117 7 coronavirus coronavirus NN cord-308950-bl83r4v3 117 8 229E 229e CD cord-308950-bl83r4v3 118 1 We -PRON- PRP cord-308950-bl83r4v3 118 2 thank thank VBP cord-308950-bl83r4v3 118 3 Dr. Dr. NNP cord-308950-bl83r4v3 118 4 Andreas Andreas NNP cord-308950-bl83r4v3 118 5 Kolb Kolb NNP cord-308950-bl83r4v3 118 6 _SP