id	sid	tid	token	lemma	pos
cord-333636-h2sg6shp	1	1	key	key	JJ
cord-333636-h2sg6shp	1	2	:	:	:
cord-333636-h2sg6shp	1	3	cord-333636-h2sg6shp	cord-333636-h2sg6shp	NN
cord-333636-h2sg6shp	1	4	authors	author	NNS
cord-333636-h2sg6shp	1	5	:	:	:
cord-333636-h2sg6shp	1	6	Kochan	Kochan	NNP
cord-333636-h2sg6shp	1	7	,	,	,
cord-333636-h2sg6shp	1	8	G.	G.	NNP
cord-333636-h2sg6shp	1	9	;	;	:
cord-333636-h2sg6shp	1	10	Gonzalez	Gonzalez	NNP
cord-333636-h2sg6shp	1	11	,	,	,
cord-333636-h2sg6shp	1	12	D.	D.	NNP
cord-333636-h2sg6shp	1	13	;	;	:
cord-333636-h2sg6shp	1	14	Rodriguez	Rodriguez	NNP
cord-333636-h2sg6shp	1	15	,	,	,
cord-333636-h2sg6shp	1	16	J.	J.	NNP
cord-333636-h2sg6shp	1	17	F.	F.	NNP
cord-333636-h2sg6shp	1	18	title	title	NN
cord-333636-h2sg6shp	1	19	:	:	:
cord-333636-h2sg6shp	2	1	Characterization	characterization	NN
cord-333636-h2sg6shp	2	2	of	of	IN
cord-333636-h2sg6shp	2	3	the	the	DT
cord-333636-h2sg6shp	2	4	RNA	RNA	NNP
cord-333636-h2sg6shp	2	5	-	-	HYPH
cord-333636-h2sg6shp	2	6	binding	bind	VBG
cord-333636-h2sg6shp	2	7	activity	activity	NN
cord-333636-h2sg6shp	2	8	of	of	IN
cord-333636-h2sg6shp	2	9	VP3	VP3	NNP
cord-333636-h2sg6shp	2	10	,	,	,
cord-333636-h2sg6shp	2	11	a	a	DT
cord-333636-h2sg6shp	2	12	major	major	JJ
cord-333636-h2sg6shp	2	13	structural	structural	JJ
cord-333636-h2sg6shp	2	14	protein	protein	NN
cord-333636-h2sg6shp	2	15	of	of	IN
cord-333636-h2sg6shp	2	16	Infectious	infectious	JJ
cord-333636-h2sg6shp	2	17	bursal	bursal	NN
cord-333636-h2sg6shp	2	18	disease	disease	NN
cord-333636-h2sg6shp	2	19	virus	virus	NN
cord-333636-h2sg6shp	2	20	date	date	NN
cord-333636-h2sg6shp	2	21	:	:	:
cord-333636-h2sg6shp	2	22	2003	2003	CD
cord-333636-h2sg6shp	2	23	journal	journal	NNP
cord-333636-h2sg6shp	2	24	:	:	:
cord-333636-h2sg6shp	3	1	Arch	Arch	NNP
cord-333636-h2sg6shp	3	2	Virol	Virol	NNP
cord-333636-h2sg6shp	4	1	DOI	DOI	NNP
cord-333636-h2sg6shp	4	2	:	:	:
cord-333636-h2sg6shp	5	1	10.1007	10.1007	CD
cord-333636-h2sg6shp	5	2	/	/	SYM
cord-333636-h2sg6shp	5	3	s00705	s00705	NNP
cord-333636-h2sg6shp	5	4	-	-	HYPH
cord-333636-h2sg6shp	5	5	002	002	CD
cord-333636-h2sg6shp	5	6	-	-	HYPH
cord-333636-h2sg6shp	5	7	0949	0949	CD
cord-333636-h2sg6shp	5	8	-	-	SYM
cord-333636-h2sg6shp	5	9	5	5	CD
cord-333636-h2sg6shp	5	10	sha	sha	NNP
cord-333636-h2sg6shp	5	11	:	:	:
cord-333636-h2sg6shp	5	12	43c608a638c99c521b349fc00e48c29acaabf4eb	43c608a638c99c521b349fc00e48c29acaabf4eb	CD
cord-333636-h2sg6shp	5	13	doc_id	doc_id	CD
cord-333636-h2sg6shp	5	14	:	:	:
cord-333636-h2sg6shp	5	15	333636	333636	CD
cord-333636-h2sg6shp	5	16	cord_uid	cord_uid	NNS
cord-333636-h2sg6shp	5	17	:	:	:
cord-333636-h2sg6shp	6	1	h2sg6shp	h2sg6shp	NFP
cord-333636-h2sg6shp	6	2	Infectious	infectious	JJ
cord-333636-h2sg6shp	6	3	bursal	bursal	NN
cord-333636-h2sg6shp	6	4	disease	disease	NN
cord-333636-h2sg6shp	6	5	virus	virus	NN
cord-333636-h2sg6shp	6	6	(	(	-LRB-
cord-333636-h2sg6shp	6	7	IBDV	IBDV	NNP
cord-333636-h2sg6shp	6	8	)	)	-RRB-
cord-333636-h2sg6shp	6	9	is	be	VBZ
cord-333636-h2sg6shp	6	10	the	the	DT
cord-333636-h2sg6shp	6	11	agent	agent	NN
cord-333636-h2sg6shp	6	12	of	of	IN
cord-333636-h2sg6shp	6	13	an	an	DT
cord-333636-h2sg6shp	6	14	immune	immune	JJ
cord-333636-h2sg6shp	6	15	-	-	HYPH
cord-333636-h2sg6shp	6	16	depressive	depressive	JJ
cord-333636-h2sg6shp	6	17	disease	disease	NN
cord-333636-h2sg6shp	6	18	affecting	affect	VBG
cord-333636-h2sg6shp	6	19	the	the	DT
cord-333636-h2sg6shp	6	20	poultry	poultry	NN
cord-333636-h2sg6shp	6	21	industry	industry	NN
cord-333636-h2sg6shp	6	22	worldwide	worldwide	RB
cord-333636-h2sg6shp	6	23	.	.	.
cord-333636-h2sg6shp	7	1	Infection	infection	NN
cord-333636-h2sg6shp	7	2	of	of	IN
cord-333636-h2sg6shp	7	3	IBDV	IBDV	NNP
cord-333636-h2sg6shp	7	4	leads	lead	VBZ
cord-333636-h2sg6shp	7	5	to	to	IN
cord-333636-h2sg6shp	7	6	expression	expression	NN
cord-333636-h2sg6shp	7	7	of	of	IN
cord-333636-h2sg6shp	7	8	five	five	CD
cord-333636-h2sg6shp	7	9	mature	mature	JJ
cord-333636-h2sg6shp	7	10	virus	virus	NN
cord-333636-h2sg6shp	7	11	-	-	HYPH
cord-333636-h2sg6shp	7	12	encoded	encode	VBN
cord-333636-h2sg6shp	7	13	proteins	protein	NNS
cord-333636-h2sg6shp	7	14	.	.	.
cord-333636-h2sg6shp	8	1	Proteolytic	proteolytic	JJ
cord-333636-h2sg6shp	8	2	processing	processing	NN
cord-333636-h2sg6shp	8	3	of	of	IN
cord-333636-h2sg6shp	8	4	the	the	DT
cord-333636-h2sg6shp	8	5	virus	virus	NN
cord-333636-h2sg6shp	8	6	-	-	HYPH
cord-333636-h2sg6shp	8	7	encoded	encode	VBN
cord-333636-h2sg6shp	8	8	polyprotein	polyprotein	NN
cord-333636-h2sg6shp	8	9	generates	generate	VBZ
cord-333636-h2sg6shp	8	10	VP3	VP3	NNP
cord-333636-h2sg6shp	8	11	which	which	WDT
cord-333636-h2sg6shp	8	12	coats	coat	VBZ
cord-333636-h2sg6shp	8	13	the	the	DT
cord-333636-h2sg6shp	8	14	inner	inner	JJ
cord-333636-h2sg6shp	8	15	surface	surface	NN
cord-333636-h2sg6shp	8	16	of	of	IN
cord-333636-h2sg6shp	8	17	the	the	DT
cord-333636-h2sg6shp	8	18	IBDV	IBDV	NNP
cord-333636-h2sg6shp	8	19	capsid	capsid	NN
cord-333636-h2sg6shp	8	20	.	.	.
cord-333636-h2sg6shp	9	1	In	in	IN
cord-333636-h2sg6shp	9	2	this	this	DT
cord-333636-h2sg6shp	9	3	report	report	NN
cord-333636-h2sg6shp	9	4	,	,	,
cord-333636-h2sg6shp	9	5	we	-PRON-	PRP
cord-333636-h2sg6shp	9	6	describe	describe	VBP
cord-333636-h2sg6shp	9	7	the	the	DT
cord-333636-h2sg6shp	9	8	characterization	characterization	NN
cord-333636-h2sg6shp	9	9	of	of	IN
cord-333636-h2sg6shp	9	10	the	the	DT
cord-333636-h2sg6shp	9	11	RNA	RNA	NNP
cord-333636-h2sg6shp	9	12	-	-	HYPH
cord-333636-h2sg6shp	9	13	binding	bind	VBG
cord-333636-h2sg6shp	9	14	activity	activity	NN
cord-333636-h2sg6shp	9	15	of	of	IN
cord-333636-h2sg6shp	9	16	VP3	VP3	NNP
cord-333636-h2sg6shp	9	17	.	.	.
cord-333636-h2sg6shp	10	1	For	for	IN
cord-333636-h2sg6shp	10	2	these	these	DT
cord-333636-h2sg6shp	10	3	studies	study	NNS
cord-333636-h2sg6shp	10	4	,	,	,
cord-333636-h2sg6shp	10	5	the	the	DT
cord-333636-h2sg6shp	10	6	VP3	VP3	NNP
cord-333636-h2sg6shp	10	7	coding	coding	NN
cord-333636-h2sg6shp	10	8	region	region	NN
cord-333636-h2sg6shp	10	9	was	be	VBD
cord-333636-h2sg6shp	10	10	fused	fuse	VBN
cord-333636-h2sg6shp	10	11	to	to	IN
cord-333636-h2sg6shp	10	12	a	a	DT
cord-333636-h2sg6shp	10	13	histidine	histidine	NN
cord-333636-h2sg6shp	10	14	tag	tag	NN
cord-333636-h2sg6shp	10	15	and	and	CC
cord-333636-h2sg6shp	10	16	expressed	express	VBN
cord-333636-h2sg6shp	10	17	in	in	IN
cord-333636-h2sg6shp	10	18	insect	insect	NN
cord-333636-h2sg6shp	10	19	cells	cell	NNS
cord-333636-h2sg6shp	10	20	using	use	VBG
cord-333636-h2sg6shp	10	21	a	a	DT
cord-333636-h2sg6shp	10	22	recombinant	recombinant	JJ
cord-333636-h2sg6shp	10	23	baculovirus	baculovirus	NN
cord-333636-h2sg6shp	10	24	.	.	.
cord-333636-h2sg6shp	11	1	The	the	DT
cord-333636-h2sg6shp	11	2	histidine	histidine	NN
cord-333636-h2sg6shp	11	3	-	-	HYPH
cord-333636-h2sg6shp	11	4	tagged	tag	VBN
cord-333636-h2sg6shp	11	5	VP3	VP3	NNP
cord-333636-h2sg6shp	11	6	was	be	VBD
cord-333636-h2sg6shp	11	7	affinity	affinity	NN
cord-333636-h2sg6shp	11	8	-	-	HYPH
cord-333636-h2sg6shp	11	9	purified	purify	VBN
cord-333636-h2sg6shp	11	10	and	and	CC
cord-333636-h2sg6shp	11	11	used	use	VBN
cord-333636-h2sg6shp	11	12	to	to	TO
cord-333636-h2sg6shp	11	13	study	study	VB
cord-333636-h2sg6shp	11	14	its	-PRON-	PRP$
cord-333636-h2sg6shp	11	15	ability	ability	NN
cord-333636-h2sg6shp	11	16	to	to	TO
cord-333636-h2sg6shp	11	17	bind	bind	VB
cord-333636-h2sg6shp	11	18	RNA	RNA	NNP
cord-333636-h2sg6shp	11	19	molecules	molecule	NNS
cord-333636-h2sg6shp	11	20	using	use	VBG
cord-333636-h2sg6shp	11	21	three	three	CD
cord-333636-h2sg6shp	11	22	complementary	complementary	JJ
cord-333636-h2sg6shp	11	23	methods	method	NNS
cord-333636-h2sg6shp	11	24	:	:	:
cord-333636-h2sg6shp	11	25	(	(	-LRB-
cord-333636-h2sg6shp	11	26	i	i	NN
cord-333636-h2sg6shp	11	27	)	)	-RRB-
cord-333636-h2sg6shp	12	1	Northwestern	northwestern	JJ
cord-333636-h2sg6shp	12	2	blotting	blotting	NN
cord-333636-h2sg6shp	13	1	;	;	:
cord-333636-h2sg6shp	13	2	(	(	-LRB-
cord-333636-h2sg6shp	13	3	ii	ii	NN
cord-333636-h2sg6shp	13	4	)	)	-RRB-
cord-333636-h2sg6shp	13	5	binding	binding	NN
cord-333636-h2sg6shp	13	6	of	of	IN
cord-333636-h2sg6shp	13	7	VP3	VP3	NNP
cord-333636-h2sg6shp	13	8	protein	protein	NN
cord-333636-h2sg6shp	13	9	-	-	HYPH
cord-333636-h2sg6shp	13	10	RNA	RNA	NNP
cord-333636-h2sg6shp	13	11	complexes	complex	NNS
cord-333636-h2sg6shp	13	12	to	to	IN
cord-333636-h2sg6shp	13	13	nitrocellulose	nitrocellulose	JJ
cord-333636-h2sg6shp	13	14	membranes	membrane	NNS
cord-333636-h2sg6shp	13	15	;	;	:
cord-333636-h2sg6shp	13	16	and	and	CC
cord-333636-h2sg6shp	13	17	(	(	-LRB-
cord-333636-h2sg6shp	13	18	iii	iii	NN
cord-333636-h2sg6shp	13	19	)	)	-RRB-
cord-333636-h2sg6shp	13	20	electrophoretic	electrophoretic	JJ
cord-333636-h2sg6shp	13	21	mobility	mobility	NN
cord-333636-h2sg6shp	13	22	shift	shift	NN
cord-333636-h2sg6shp	13	23	assays	assay	NNS
cord-333636-h2sg6shp	13	24	.	.	.
cord-333636-h2sg6shp	14	1	The	the	DT
cord-333636-h2sg6shp	14	2	results	result	NNS
cord-333636-h2sg6shp	14	3	demonstrated	demonstrate	VBD
cord-333636-h2sg6shp	14	4	that	that	IN
cord-333636-h2sg6shp	14	5	VP3	VP3	NNP
cord-333636-h2sg6shp	14	6	efficiently	efficiently	RB
cord-333636-h2sg6shp	14	7	bound	bind	VBN
cord-333636-h2sg6shp	14	8	ssRNA	ssRNA	''
cord-333636-h2sg6shp	15	1	and	and	CC
cord-333636-h2sg6shp	15	2	dsRNA	dsrna	NN
cord-333636-h2sg6shp	15	3	.	.	.
cord-333636-h2sg6shp	16	1	Under	under	IN
cord-333636-h2sg6shp	16	2	the	the	DT
cord-333636-h2sg6shp	16	3	experimental	experimental	JJ
cord-333636-h2sg6shp	16	4	conditions	condition	NNS
cord-333636-h2sg6shp	16	5	used	use	VBN
cord-333636-h2sg6shp	16	6	in	in	IN
cord-333636-h2sg6shp	16	7	this	this	DT
cord-333636-h2sg6shp	16	8	study	study	NN
cord-333636-h2sg6shp	16	9	,	,	,
cord-333636-h2sg6shp	16	10	the	the	DT
cord-333636-h2sg6shp	16	11	formation	formation	NN
cord-333636-h2sg6shp	16	12	of	of	IN
cord-333636-h2sg6shp	16	13	VP3-RNA	VP3-RNA	NNP
cord-333636-h2sg6shp	16	14	complexes	complex	NNS
cord-333636-h2sg6shp	16	15	did	do	VBD
cord-333636-h2sg6shp	16	16	not	not	RB
cord-333636-h2sg6shp	16	17	depend	depend	VB
cord-333636-h2sg6shp	16	18	upon	upon	IN
cord-333636-h2sg6shp	16	19	the	the	DT
cord-333636-h2sg6shp	16	20	presence	presence	NN
cord-333636-h2sg6shp	16	21	of	of	IN
cord-333636-h2sg6shp	16	22	specific	specific	JJ
cord-333636-h2sg6shp	16	23	RNA	RNA	NNP
cord-333636-h2sg6shp	16	24	sequences	sequence	NNS
cord-333636-h2sg6shp	16	25	.	.	.
cord-333636-h2sg6shp	17	1	A	a	DT
cord-333636-h2sg6shp	17	2	series	series	NN
cord-333636-h2sg6shp	17	3	of	of	IN
cord-333636-h2sg6shp	17	4	histidine	histidine	NN
cord-333636-h2sg6shp	17	5	-	-	HYPH
cord-333636-h2sg6shp	17	6	tagged	tag	VBN
cord-333636-h2sg6shp	17	7	VP3	VP3	NNP
cord-333636-h2sg6shp	17	8	deletion	deletion	NN
cord-333636-h2sg6shp	17	9	mutants	mutant	NNS
cord-333636-h2sg6shp	17	10	spanning	span	VBG
cord-333636-h2sg6shp	17	11	the	the	DT
cord-333636-h2sg6shp	17	12	whole	whole	JJ
cord-333636-h2sg6shp	17	13	VP3	vp3	JJ
cord-333636-h2sg6shp	17	14	coding	coding	NN
cord-333636-h2sg6shp	17	15	region	region	NN
cord-333636-h2sg6shp	17	16	were	be	VBD
cord-333636-h2sg6shp	17	17	generated	generate	VBN
cord-333636-h2sg6shp	17	18	.	.	.
cord-333636-h2sg6shp	18	1	The	the	DT
cord-333636-h2sg6shp	18	2	use	use	NN
cord-333636-h2sg6shp	18	3	of	of	IN
cord-333636-h2sg6shp	18	4	these	these	DT
cord-333636-h2sg6shp	18	5	mutants	mutant	NNS
cord-333636-h2sg6shp	18	6	revealed	reveal	VBD
cord-333636-h2sg6shp	18	7	that	that	IN
cord-333636-h2sg6shp	18	8	the	the	DT
cord-333636-h2sg6shp	18	9	VP3	VP3	NNP
cord-333636-h2sg6shp	18	10	RNA	RNA	NNP
cord-333636-h2sg6shp	18	11	-	-	HYPH
cord-333636-h2sg6shp	18	12	binding	bind	VBG
cord-333636-h2sg6shp	18	13	domain	domain	NN
cord-333636-h2sg6shp	18	14	layed	lay	VBN
cord-333636-h2sg6shp	18	15	in	in	IN
cord-333636-h2sg6shp	18	16	a	a	DT
cord-333636-h2sg6shp	18	17	highly	highly	RB
cord-333636-h2sg6shp	18	18	conserved	conserved	JJ
cord-333636-h2sg6shp	18	19	69	69	CD
cord-333636-h2sg6shp	18	20	aa	aa	NNP
cord-333636-h2sg6shp	18	21	stretch	stretch	NN
cord-333636-h2sg6shp	18	22	close	close	RB
cord-333636-h2sg6shp	18	23	to	to	IN
cord-333636-h2sg6shp	18	24	the	the	DT
cord-333636-h2sg6shp	18	25	N	n	NN
cord-333636-h2sg6shp	18	26	-	-	HYPH
cord-333636-h2sg6shp	18	27	terminus	terminus	NN
cord-333636-h2sg6shp	18	28	of	of	IN
cord-333636-h2sg6shp	18	29	the	the	DT
cord-333636-h2sg6shp	18	30	protein	protein	NN
cord-333636-h2sg6shp	18	31	.	.	.
cord-333636-h2sg6shp	19	1	Avibirnavirus	Avibirnavirus	NNP
cord-333636-h2sg6shp	19	2	of	of	IN
cord-333636-h2sg6shp	19	3	the	the	DT
cord-333636-h2sg6shp	19	4	family	family	NN
cord-333636-h2sg6shp	19	5	Birnaviridae	Birnaviridae	NNP
cord-333636-h2sg6shp	19	6	[	[	-LRB-
cord-333636-h2sg6shp	19	7	27	27	CD
cord-333636-h2sg6shp	19	8	]	]	-RRB-
cord-333636-h2sg6shp	19	9	.	.	.
cord-333636-h2sg6shp	20	1	Infection	infection	NN
cord-333636-h2sg6shp	20	2	of	of	IN
cord-333636-h2sg6shp	20	3	young	young	JJ
cord-333636-h2sg6shp	20	4	chickens	chicken	NNS
cord-333636-h2sg6shp	20	5	with	with	IN
cord-333636-h2sg6shp	20	6	IBDV	IBDV	NNP
cord-333636-h2sg6shp	20	7	leads	lead	VBZ
cord-333636-h2sg6shp	20	8	to	to	IN
cord-333636-h2sg6shp	20	9	infectious	infectious	JJ
cord-333636-h2sg6shp	20	10	bursal	bursal	NN
cord-333636-h2sg6shp	20	11	disease	disease	NN
cord-333636-h2sg6shp	20	12	(	(	-LRB-
cord-333636-h2sg6shp	20	13	IBD	IBD	NNP
cord-333636-h2sg6shp	20	14	)	)	-RRB-
cord-333636-h2sg6shp	20	15	,	,	,
cord-333636-h2sg6shp	20	16	characterized	characterize	VBN
cord-333636-h2sg6shp	20	17	by	by	IN
cord-333636-h2sg6shp	20	18	the	the	DT
cord-333636-h2sg6shp	20	19	destruction	destruction	NN
cord-333636-h2sg6shp	20	20	of	of	IN
cord-333636-h2sg6shp	20	21	the	the	DT
cord-333636-h2sg6shp	20	22	bursa	bursa	NN
cord-333636-h2sg6shp	20	23	of	of	IN
cord-333636-h2sg6shp	20	24	Fabricious	Fabricious	NNP
cord-333636-h2sg6shp	20	25	that	that	WDT
cord-333636-h2sg6shp	20	26	results	result	VBZ
cord-333636-h2sg6shp	20	27	in	in	IN
cord-333636-h2sg6shp	20	28	a	a	DT
cord-333636-h2sg6shp	20	29	severe	severe	JJ
cord-333636-h2sg6shp	20	30	immunosuppression	immunosuppression	NN
cord-333636-h2sg6shp	20	31	.	.	.
cord-333636-h2sg6shp	21	1	IBD	IBD	NNP
cord-333636-h2sg6shp	21	2	causes	cause	VBZ
cord-333636-h2sg6shp	21	3	major	major	JJ
cord-333636-h2sg6shp	21	4	economical	economical	JJ
cord-333636-h2sg6shp	21	5	losses	loss	NNS
cord-333636-h2sg6shp	21	6	to	to	IN
cord-333636-h2sg6shp	21	7	the	the	DT
cord-333636-h2sg6shp	21	8	poultry	poultry	NN
cord-333636-h2sg6shp	21	9	industry	industry	NN
cord-333636-h2sg6shp	21	10	[	[	-LRB-
cord-333636-h2sg6shp	21	11	34	34	CD
cord-333636-h2sg6shp	21	12	,	,	,
cord-333636-h2sg6shp	21	13	39	39	CD
cord-333636-h2sg6shp	21	14	]	]	-RRB-
cord-333636-h2sg6shp	21	15	and	and	CC
cord-333636-h2sg6shp	21	16	it	-PRON-	PRP
cord-333636-h2sg6shp	21	17	might	may	MD
cord-333636-h2sg6shp	21	18	also	also	RB
cord-333636-h2sg6shp	21	19	affect	affect	VB
cord-333636-h2sg6shp	21	20	several	several	JJ
cord-333636-h2sg6shp	21	21	species	specie	NNS
cord-333636-h2sg6shp	21	22	of	of	IN
cord-333636-h2sg6shp	21	23	wild	wild	JJ
cord-333636-h2sg6shp	21	24	birds	bird	NNS
cord-333636-h2sg6shp	21	25	[	[	-LRB-
cord-333636-h2sg6shp	21	26	18	18	CD
cord-333636-h2sg6shp	21	27	,	,	,
cord-333636-h2sg6shp	21	28	35	35	CD
cord-333636-h2sg6shp	21	29	,	,	,
cord-333636-h2sg6shp	21	30	45	45	CD
cord-333636-h2sg6shp	21	31	]	]	-RRB-
cord-333636-h2sg6shp	21	32	.	.	.
cord-333636-h2sg6shp	22	1	IBDV	IBDV	NNP
cord-333636-h2sg6shp	22	2	VP3	VP3	NNP
cord-333636-h2sg6shp	22	3	coding	code	VBG
cord-333636-h2sg6shp	22	4	sequence	sequence	NN
cord-333636-h2sg6shp	22	5	,	,	,
cord-333636-h2sg6shp	22	6	corresponding	correspond	VBG
cord-333636-h2sg6shp	22	7	to	to	IN
cord-333636-h2sg6shp	22	8	polyprotein	polyprotein	NNP
cord-333636-h2sg6shp	22	9	aa	aa	NNP
cord-333636-h2sg6shp	22	10	positions	position	VBZ
cord-333636-h2sg6shp	22	11	756	756	CD
cord-333636-h2sg6shp	22	12	to	to	IN
cord-333636-h2sg6shp	22	13	1012	1012	CD
cord-333636-h2sg6shp	22	14	,	,	,
cord-333636-h2sg6shp	22	15	was	be	VBD
cord-333636-h2sg6shp	22	16	subcloned	subclone	VBN
cord-333636-h2sg6shp	22	17	from	from	IN
cord-333636-h2sg6shp	22	18	plasmid	plasmid	NN
cord-333636-h2sg6shp	23	1	pVOTE1	pVOTE1	NNP
cord-333636-h2sg6shp	23	2	/	/	SYM
cord-333636-h2sg6shp	23	3	POLY	POLY	NNP
cord-333636-h2sg6shp	23	4	[	[	-LRB-
cord-333636-h2sg6shp	23	5	16	16	CD
cord-333636-h2sg6shp	23	6	]	]	-RRB-
cord-333636-h2sg6shp	23	7	into	into	IN
cord-333636-h2sg6shp	23	8	the	the	DT
cord-333636-h2sg6shp	23	9	pFastBac	pfastbac	JJ
cord-333636-h2sg6shp	23	10	HTc	HTc	NNS
cord-333636-h2sg6shp	23	11	vector	vector	NN
cord-333636-h2sg6shp	23	12	(	(	-LRB-
cord-333636-h2sg6shp	23	13	Gibco	Gibco	NNP
cord-333636-h2sg6shp	23	14	BRL	BRL	NNP
cord-333636-h2sg6shp	23	15	)	)	-RRB-
cord-333636-h2sg6shp	23	16	.	.	.
cord-333636-h2sg6shp	24	1	Briefly	briefly	RB
cord-333636-h2sg6shp	24	2	,	,	,
cord-333636-h2sg6shp	24	3	pVOTE1	pVOTE1	NNP
cord-333636-h2sg6shp	24	4	/	/	SYM
cord-333636-h2sg6shp	24	5	POLY	POLY	NNP
cord-333636-h2sg6shp	24	6	cDNA	cdna	NN
cord-333636-h2sg6shp	24	7	was	be	VBD
cord-333636-h2sg6shp	24	8	digested	digest	VBN
cord-333636-h2sg6shp	24	9	with	with	IN
cord-333636-h2sg6shp	24	10	BamHI	bamhi	NN
cord-333636-h2sg6shp	24	11	and	and	CC
cord-333636-h2sg6shp	24	12	partially	partially	RB
cord-333636-h2sg6shp	24	13	with	with	IN
cord-333636-h2sg6shp	24	14	NcoI	NcoI	NNP
cord-333636-h2sg6shp	24	15	,	,	,
cord-333636-h2sg6shp	24	16	and	and	CC
cord-333636-h2sg6shp	24	17	the	the	DT
cord-333636-h2sg6shp	24	18	resulting	result	VBG
cord-333636-h2sg6shp	24	19	DNA	dna	NN
cord-333636-h2sg6shp	24	20	fragment	fragment	NN
cord-333636-h2sg6shp	24	21	containing	contain	VBG
cord-333636-h2sg6shp	24	22	the	the	DT
cord-333636-h2sg6shp	24	23	VP3	VP3	NNP
cord-333636-h2sg6shp	24	24	gene	gene	NN
cord-333636-h2sg6shp	24	25	was	be	VBD
cord-333636-h2sg6shp	24	26	ligated	ligate	VBN
cord-333636-h2sg6shp	24	27	to	to	IN
cord-333636-h2sg6shp	24	28	plasmid	plasmid	NN
cord-333636-h2sg6shp	24	29	pFastBac	pfastbac	JJ
cord-333636-h2sg6shp	24	30	HTc	HTc	NNS
cord-333636-h2sg6shp	24	31	restricted	restrict	VBN
cord-333636-h2sg6shp	24	32	with	with	IN
cord-333636-h2sg6shp	24	33	the	the	DT
cord-333636-h2sg6shp	24	34	same	same	JJ
cord-333636-h2sg6shp	24	35	enzymes	enzyme	NNS
cord-333636-h2sg6shp	24	36	,	,	,
cord-333636-h2sg6shp	24	37	generating	generate	VBG
cord-333636-h2sg6shp	24	38	the	the	DT
cord-333636-h2sg6shp	24	39	plasmid	plasmid	NN
cord-333636-h2sg6shp	24	40	pFastBac	pfastbac	JJ
cord-333636-h2sg6shp	24	41	-	-	HYPH
cord-333636-h2sg6shp	24	42	VP3-His	vp3-his	NN
cord-333636-h2sg6shp	24	43	.	.	.
cord-333636-h2sg6shp	25	1	This	this	DT
cord-333636-h2sg6shp	25	2	plasmid	plasmid	NN
cord-333636-h2sg6shp	25	3	contained	contain	VBD
cord-333636-h2sg6shp	25	4	the	the	DT
cord-333636-h2sg6shp	25	5	VP3	VP3	NNP
cord-333636-h2sg6shp	25	6	gene	gene	NN
cord-333636-h2sg6shp	25	7	fused	fuse	VBN
cord-333636-h2sg6shp	25	8	to	to	IN
cord-333636-h2sg6shp	25	9	a	a	DT
cord-333636-h2sg6shp	25	10	histidine	histidine	JJ
cord-333636-h2sg6shp	25	11	tag	tag	NN
cord-333636-h2sg6shp	25	12	under	under	IN
cord-333636-h2sg6shp	25	13	the	the	DT
cord-333636-h2sg6shp	25	14	control	control	NN
cord-333636-h2sg6shp	25	15	of	of	IN
cord-333636-h2sg6shp	25	16	the	the	DT
cord-333636-h2sg6shp	25	17	polyhedrin	polyhedrin	NN
cord-333636-h2sg6shp	25	18	promoter	promoter	NN
cord-333636-h2sg6shp	25	19	.	.	.
cord-333636-h2sg6shp	26	1	VP3	VP3	NNP
cord-333636-h2sg6shp	26	2	deletion	deletion	NN
cord-333636-h2sg6shp	26	3	mutants	mutant	NNS
cord-333636-h2sg6shp	26	4	were	be	VBD
cord-333636-h2sg6shp	26	5	generated	generate	VBN
cord-333636-h2sg6shp	26	6	by	by	IN
cord-333636-h2sg6shp	26	7	overlap	overlap	NN
cord-333636-h2sg6shp	26	8	extension	extension	NN
cord-333636-h2sg6shp	26	9	PCR	PCR	NNP
cord-333636-h2sg6shp	26	10	[	[	-LRB-
cord-333636-h2sg6shp	26	11	36	36	CD
cord-333636-h2sg6shp	26	12	]	]	-RRB-
cord-333636-h2sg6shp	26	13	using	use	VBG
cord-333636-h2sg6shp	26	14	synthetic	synthetic	JJ
cord-333636-h2sg6shp	26	15	primers	primer	NNS
cord-333636-h2sg6shp	26	16	containing	contain	VBG
cord-333636-h2sg6shp	26	17	the	the	DT
cord-333636-h2sg6shp	26	18	desired	desire	VBN
cord-333636-h2sg6shp	26	19	deletions	deletion	NNS
cord-333636-h2sg6shp	26	20	and	and	CC
cord-333636-h2sg6shp	26	21	using	use	VBG
cord-333636-h2sg6shp	26	22	the	the	DT
cord-333636-h2sg6shp	26	23	pcDNA3-POLY	pcdna3-poly	CD
cord-333636-h2sg6shp	26	24	plasmid	plasmid	NN
cord-333636-h2sg6shp	26	25	[	[	-LRB-
cord-333636-h2sg6shp	26	26	40	40	CD
cord-333636-h2sg6shp	26	27	]	]	-RRB-
cord-333636-h2sg6shp	26	28	as	as	IN
cord-333636-h2sg6shp	26	29	a	a	DT
cord-333636-h2sg6shp	26	30	template	template	NN
cord-333636-h2sg6shp	26	31	.	.	.
cord-333636-h2sg6shp	27	1	PCR	PCR	NNP
cord-333636-h2sg6shp	27	2	products	product	NNS
cord-333636-h2sg6shp	27	3	were	be	VBD
cord-333636-h2sg6shp	27	4	obtained	obtain	VBN
cord-333636-h2sg6shp	27	5	either	either	CC
cord-333636-h2sg6shp	27	6	with	with	IN
cord-333636-h2sg6shp	27	7	primer	primer	NN
cord-333636-h2sg6shp	27	8	A	a	NN
cord-333636-h2sg6shp	27	9	(	(	-LRB-
cord-333636-h2sg6shp	27	10	5	5	CD
cord-333636-h2sg6shp	27	11	-GCGCGAGCTCCGTTTCCCTC	-GCGCGAGCTCCGTTTCCCTC	NNP
cord-333636-h2sg6shp	27	12	ACAATCCACGCGAC-3	ACAATCCACGCGAC-3	NNP
cord-333636-h2sg6shp	27	13	)	)	-RRB-
cord-333636-h2sg6shp	27	14	with	with	IN
cord-333636-h2sg6shp	27	15	the	the	DT
cord-333636-h2sg6shp	27	16	corresponding	corresponding	JJ
cord-333636-h2sg6shp	27	17	reverse	reverse	JJ
cord-333636-h2sg6shp	27	18	-	-	HYPH
cord-333636-h2sg6shp	27	19	sense	sense	NN
cord-333636-h2sg6shp	27	20	primer	primer	NN
cord-333636-h2sg6shp	27	21	(	(	-LRB-
cord-333636-h2sg6shp	27	22	Table	table	NN
cord-333636-h2sg6shp	27	23	1	1	CD
cord-333636-h2sg6shp	27	24	)	)	-RRB-
cord-333636-h2sg6shp	27	25	or	or	CC
cord-333636-h2sg6shp	27	26	with	with	IN
cord-333636-h2sg6shp	27	27	primer	primer	NN
cord-333636-h2sg6shp	27	28	B	b	NN
cord-333636-h2sg6shp	27	29	(	(	-LRB-
cord-333636-h2sg6shp	27	30	5	5	CD
cord-333636-h2sg6shp	27	31	-GCGCGGATCCTCATCCAAGGTCCTCATC	-GCGCGGATCCTCATCCAAGGTCCTCATC	NNP
cord-333636-h2sg6shp	27	32	AGAGACA-3	AGAGACA-3	NNP
cord-333636-h2sg6shp	27	33	)	)	-RRB-
cord-333636-h2sg6shp	27	34	with	with	IN
cord-333636-h2sg6shp	27	35	the	the	DT
cord-333636-h2sg6shp	27	36	corresponding	corresponding	JJ
cord-333636-h2sg6shp	27	37	reverse	reverse	JJ
cord-333636-h2sg6shp	27	38	-	-	HYPH
cord-333636-h2sg6shp	27	39	sense	sense	NN
cord-333636-h2sg6shp	27	40	primer	primer	NN
cord-333636-h2sg6shp	27	41	(	(	-LRB-
cord-333636-h2sg6shp	27	42	Table	table	NN
cord-333636-h2sg6shp	27	43	1	1	CD
cord-333636-h2sg6shp	27	44	)	)	-RRB-
cord-333636-h2sg6shp	27	45	.	.	.
cord-333636-h2sg6shp	28	1	Both	both	DT
cord-333636-h2sg6shp	28	2	products	product	NNS
cord-333636-h2sg6shp	28	3	were	be	VBD
cord-333636-h2sg6shp	28	4	recombined	recombine	VBN
cord-333636-h2sg6shp	28	5	by	by	IN
cord-333636-h2sg6shp	28	6	PCR	PCR	NNP
cord-333636-h2sg6shp	28	7	using	use	VBG
cord-333636-h2sg6shp	28	8	primers	primer	NNS
cord-333636-h2sg6shp	28	9	A	A	NNP
cord-333636-h2sg6shp	28	10	and	and	CC
cord-333636-h2sg6shp	28	11	B.	B.	NNP
cord-333636-h2sg6shp	28	12	PCR	PCR	NNP
cord-333636-h2sg6shp	28	13	products	product	NNS
cord-333636-h2sg6shp	28	14	were	be	VBD
cord-333636-h2sg6shp	28	15	digested	digest	VBN
cord-333636-h2sg6shp	28	16	with	with	IN
cord-333636-h2sg6shp	28	17	BamHI	BamHI	NNP
cord-333636-h2sg6shp	28	18	and	and	CC
cord-333636-h2sg6shp	28	19	XhoI	XhoI	NNP
cord-333636-h2sg6shp	28	20	and	and	CC
cord-333636-h2sg6shp	28	21	cloned	clone	VBN
cord-333636-h2sg6shp	28	22	into	into	IN
cord-333636-h2sg6shp	28	23	the	the	DT
cord-333636-h2sg6shp	28	24	pFastBac	pfastbac	JJ
cord-333636-h2sg6shp	28	25	-	-	HYPH
cord-333636-h2sg6shp	28	26	VP3-His	vp3-his	NN
cord-333636-h2sg6shp	28	27	plasmid	plasmid	NN
cord-333636-h2sg6shp	28	28	restricted	restrict	VBN
cord-333636-h2sg6shp	28	29	with	with	IN
cord-333636-h2sg6shp	28	30	the	the	DT
cord-333636-h2sg6shp	28	31	same	same	JJ
cord-333636-h2sg6shp	28	32	enzymes	enzyme	NNS
cord-333636-h2sg6shp	28	33	.	.	.
cord-333636-h2sg6shp	29	1	In	in	IN
cord-333636-h2sg6shp	29	2	this	this	DT
cord-333636-h2sg6shp	29	3	way	way	NN
cord-333636-h2sg6shp	29	4	,	,	,
cord-333636-h2sg6shp	29	5	a	a	DT
cord-333636-h2sg6shp	29	6	series	series	NN
cord-333636-h2sg6shp	29	7	of	of	IN
cord-333636-h2sg6shp	29	8	VP3	VP3	NNP
cord-333636-h2sg6shp	29	9	mutants	mutant	NNS
cord-333636-h2sg6shp	29	10	containing	contain	VBG
cord-333636-h2sg6shp	29	11	small	small	JJ
cord-333636-h2sg6shp	29	12	deletions	deletion	NNS
cord-333636-h2sg6shp	29	13	(	(	-LRB-
cord-333636-h2sg6shp	29	14	≈	≈	NNP
cord-333636-h2sg6shp	29	15	75	75	CD
cord-333636-h2sg6shp	29	16	bp	bp	NNP
cord-333636-h2sg6shp	29	17	)	)	-RRB-
cord-333636-h2sg6shp	29	18	spanning	span	VBG
cord-333636-h2sg6shp	29	19	the	the	DT
cord-333636-h2sg6shp	29	20	whole	whole	JJ
cord-333636-h2sg6shp	29	21	coding	code	VBG
cord-333636-h2sg6shp	29	22	region	region	NN
cord-333636-h2sg6shp	29	23	of	of	IN
cord-333636-h2sg6shp	29	24	the	the	DT
cord-333636-h2sg6shp	29	25	protein	protein	NN
cord-333636-h2sg6shp	29	26	was	be	VBD
cord-333636-h2sg6shp	29	27	generated	generate	VBN
cord-333636-h2sg6shp	29	28	(	(	-LRB-
cord-333636-h2sg6shp	29	29	Table	table	NN
cord-333636-h2sg6shp	29	30	1	1	CD
cord-333636-h2sg6shp	29	31	)	)	-RRB-
cord-333636-h2sg6shp	29	32	.	.	.
cord-333636-h2sg6shp	30	1	The	the	DT
cord-333636-h2sg6shp	30	2	carboxy	carboxy	JJ
cord-333636-h2sg6shp	30	3	-	-	HYPH
cord-333636-h2sg6shp	30	4	terminal	terminal	NN
cord-333636-h2sg6shp	30	5	deletion	deletion	NN
cord-333636-h2sg6shp	30	6	mutants	mutant	NNS
cord-333636-h2sg6shp	30	7	VP3	VP3	NNP
cord-333636-h2sg6shp	30	8	1008	1008	CD
cord-333636-h2sg6shp	30	9	-	-	SYM
cord-333636-h2sg6shp	30	10	1012	1012	CD
cord-333636-h2sg6shp	31	1	(	(	-LRB-
cord-333636-h2sg6shp	31	2	numbers	number	NNS
cord-333636-h2sg6shp	31	3	represent	represent	VBP
cord-333636-h2sg6shp	31	4	positions	position	NNS
cord-333636-h2sg6shp	31	5	of	of	IN
cord-333636-h2sg6shp	31	6	aminoacids	aminoacid	NNS
cord-333636-h2sg6shp	31	7	in	in	IN
cord-333636-h2sg6shp	31	8	the	the	DT
cord-333636-h2sg6shp	31	9	polyprotein	polyprotein	NN
cord-333636-h2sg6shp	31	10	,	,	,
cord-333636-h2sg6shp	31	11	flanking	flank	VBG
cord-333636-h2sg6shp	31	12	the	the	DT
cord-333636-h2sg6shp	31	13	deletions	deletion	NNS
cord-333636-h2sg6shp	31	14	)	)	-RRB-
cord-333636-h2sg6shp	31	15	and	and	CC
cord-333636-h2sg6shp	31	16	VP3	VP3	NNP
cord-333636-h2sg6shp	31	17	971	971	CD
cord-333636-h2sg6shp	31	18	-	-	HYPH
cord-333636-h2sg6shp	31	19	1012	1012	CD
cord-333636-h2sg6shp	31	20	presenting	present	VBG
cord-333636-h2sg6shp	31	21	deletions	deletion	NNS
cord-333636-h2sg6shp	31	22	of	of	IN
cord-333636-h2sg6shp	31	23	5	5	CD
cord-333636-h2sg6shp	31	24	and	and	CC
cord-333636-h2sg6shp	31	25	42	42	CD
cord-333636-h2sg6shp	31	26	residues	residue	NNS
cord-333636-h2sg6shp	31	27	,	,	,
cord-333636-h2sg6shp	31	28	respectively	respectively	RB
cord-333636-h2sg6shp	31	29	,	,	,
cord-333636-h2sg6shp	31	30	were	be	VBD
cord-333636-h2sg6shp	31	31	obtained	obtain	VBN
cord-333636-h2sg6shp	31	32	by	by	IN
cord-333636-h2sg6shp	31	33	cloning	clone	VBG
cord-333636-h2sg6shp	31	34	the	the	DT
cord-333636-h2sg6shp	31	35	VP3	VP3	NNP
cord-333636-h2sg6shp	31	36	fragment	fragment	NN
cord-333636-h2sg6shp	31	37	resulting	result	VBG
cord-333636-h2sg6shp	31	38	from	from	IN
cord-333636-h2sg6shp	31	39	digestion	digestion	NN
cord-333636-h2sg6shp	31	40	with	with	IN
cord-333636-h2sg6shp	31	41	XbaI	XbaI	NNP
cord-333636-h2sg6shp	31	42	and	and	CC
cord-333636-h2sg6shp	31	43	NotI	NotI	NNP
cord-333636-h2sg6shp	31	44	from	from	IN
cord-333636-h2sg6shp	31	45	plasmids	plasmid	NNS
cord-333636-h2sg6shp	31	46	pcDNA3-VP3	pcdna3-vp3	JJ
cord-333636-h2sg6shp	31	47	1008	1008	CD
cord-333636-h2sg6shp	31	48	-	-	SYM
cord-333636-h2sg6shp	31	49	1012	1012	CD
cord-333636-h2sg6shp	31	50	and	and	CC
cord-333636-h2sg6shp	31	51	pcDNA3-VP3	pcdna3-vp3	JJ
cord-333636-h2sg6shp	31	52	971	971	CD
cord-333636-h2sg6shp	31	53	-	-	CD
cord-333636-h2sg6shp	31	54	1012	1012	CD
cord-333636-h2sg6shp	32	1	(	(	-LRB-
cord-333636-h2sg6shp	32	2	Maraver	Maraver	NNP
cord-333636-h2sg6shp	32	3	et	et	NNP
cord-333636-h2sg6shp	32	4	al	al	NNP
cord-333636-h2sg6shp	32	5	.	.	.
cord-333636-h2sg6shp	33	1	,	,	,
cord-333636-h2sg6shp	33	2	submitted	submit	VBN
cord-333636-h2sg6shp	33	3	)	)	-RRB-
cord-333636-h2sg6shp	33	4	,	,	,
cord-333636-h2sg6shp	33	5	respectively	respectively	RB
cord-333636-h2sg6shp	33	6	,	,	,
cord-333636-h2sg6shp	33	7	into	into	IN
cord-333636-h2sg6shp	33	8	the	the	DT
cord-333636-h2sg6shp	33	9	pFastBact	pfastbact	NN
cord-333636-h2sg6shp	33	10	-	-	HYPH
cord-333636-h2sg6shp	33	11	HTc	htc	NN
cord-333636-h2sg6shp	33	12	vector	vector	NN
cord-333636-h2sg6shp	33	13	.	.	.
cord-333636-h2sg6shp	34	1	All	all	DT
cord-333636-h2sg6shp	34	2	constructs	construct	NNS
cord-333636-h2sg6shp	34	3	were	be	VBD
cord-333636-h2sg6shp	34	4	sequenced	sequence	VBN
cord-333636-h2sg6shp	34	5	to	to	TO
cord-333636-h2sg6shp	34	6	confirm	confirm	VB
cord-333636-h2sg6shp	34	7	the	the	DT
cord-333636-h2sg6shp	34	8	mutations	mutation	NNS
cord-333636-h2sg6shp	34	9	.	.	.
cord-333636-h2sg6shp	35	1	To	to	TO
cord-333636-h2sg6shp	35	2	study	study	VB
cord-333636-h2sg6shp	35	3	VP3	VP3	NNP
cord-333636-h2sg6shp	35	4	RNA	RNA	NNP
cord-333636-h2sg6shp	35	5	-	-	HYPH
cord-333636-h2sg6shp	35	6	binding	bind	VBG
cord-333636-h2sg6shp	35	7	activity	activity	NN
cord-333636-h2sg6shp	35	8	,	,	,
cord-333636-h2sg6shp	35	9	different	different	JJ
cord-333636-h2sg6shp	35	10	[	[	-LRB-
cord-333636-h2sg6shp	35	11	∝	∝	NNP
cord-333636-h2sg6shp	35	12	-32	-32	NNP
cord-333636-h2sg6shp	35	13	P]-labeled	p]-labele	VBN
cord-333636-h2sg6shp	35	14	single	single	RB
cord-333636-h2sg6shp	35	15	-	-	HYPH
cord-333636-h2sg6shp	35	16	stranded	strand	VBN
cord-333636-h2sg6shp	35	17	RNA	RNA	NNP
cord-333636-h2sg6shp	35	18	(	(	-LRB-
cord-333636-h2sg6shp	35	19	ssRNA	ssrna	NN
cord-333636-h2sg6shp	35	20	)	)	-RRB-
cord-333636-h2sg6shp	35	21	probes	probe	NNS
cord-333636-h2sg6shp	35	22	,	,	,
cord-333636-h2sg6shp	35	23	containing	contain	VBG
cord-333636-h2sg6shp	35	24	viral	viral	JJ
cord-333636-h2sg6shp	35	25	and	and	CC
cord-333636-h2sg6shp	35	26	non	non	JJ
cord-333636-h2sg6shp	35	27	-	-	JJ
cord-333636-h2sg6shp	35	28	viral	viral	JJ
cord-333636-h2sg6shp	35	29	sequences	sequence	NNS
cord-333636-h2sg6shp	35	30	,	,	,
cord-333636-h2sg6shp	35	31	were	be	VBD
cord-333636-h2sg6shp	35	32	generated	generate	VBN
cord-333636-h2sg6shp	35	33	using	use	VBG
cord-333636-h2sg6shp	35	34	the	the	DT
cord-333636-h2sg6shp	35	35	Ribo	Ribo	NNP
cord-333636-h2sg6shp	35	36	-	-	HYPH
cord-333636-h2sg6shp	35	37	Max	Max	NNP
cord-333636-h2sg6shp	35	38	kit	kit	NN
cord-333636-h2sg6shp	35	39	(	(	-LRB-
cord-333636-h2sg6shp	35	40	Promega	Promega	NNP
cord-333636-h2sg6shp	35	41	)	)	-RRB-
cord-333636-h2sg6shp	35	42	.	.	.
cord-333636-h2sg6shp	36	1	Briefly	briefly	RB
cord-333636-h2sg6shp	36	2	,	,	,
cord-333636-h2sg6shp	36	3	transcription	transcription	NN
cord-333636-h2sg6shp	36	4	reactions	reaction	NNS
cord-333636-h2sg6shp	36	5	were	be	VBD
cord-333636-h2sg6shp	36	6	carried	carry	VBN
cord-333636-h2sg6shp	36	7	out	out	RP
cord-333636-h2sg6shp	36	8	by	by	IN
cord-333636-h2sg6shp	36	9	the	the	DT
cord-333636-h2sg6shp	36	10	T7	T7	NNP
cord-333636-h2sg6shp	36	11	RNA	RNA	NNP
cord-333636-h2sg6shp	36	12	polymerase	polymerase	NN
cord-333636-h2sg6shp	36	13	for	for	IN
cord-333636-h2sg6shp	36	14	4	4	CD
cord-333636-h2sg6shp	36	15	h	h	NN
cord-333636-h2sg6shp	36	16	at	at	IN
cord-333636-h2sg6shp	36	17	37	37	CD
cord-333636-h2sg6shp	37	1	•	•	NNP
cord-333636-h2sg6shp	38	1	C.	C.	NNP
cord-333636-h2sg6shp	38	2	Radioactive	Radioactive	NNP
cord-333636-h2sg6shp	38	3	labeling	labeling	NN
cord-333636-h2sg6shp	38	4	was	be	VBD
cord-333636-h2sg6shp	38	5	introduced	introduce	VBN
cord-333636-h2sg6shp	38	6	including	include	VBG
cord-333636-h2sg6shp	38	7	10	10	CD
cord-333636-h2sg6shp	38	8	µCi[∝	µCi[∝	NNP
cord-333636-h2sg6shp	38	9	-32	-32	NNP
cord-333636-h2sg6shp	38	10	P]ATP	P]ATP	NNP
cord-333636-h2sg6shp	38	11	(	(	-LRB-
cord-333636-h2sg6shp	38	12	3000	3000	CD
cord-333636-h2sg6shp	38	13	Ci	ci	NN
cord-333636-h2sg6shp	38	14	/	/	SYM
cord-333636-h2sg6shp	38	15	mmol	mmol	NN
cord-333636-h2sg6shp	38	16	)	)	-RRB-
cord-333636-h2sg6shp	38	17	in	in	IN
cord-333636-h2sg6shp	38	18	transcription	transcription	NN
cord-333636-h2sg6shp	38	19	reactions	reaction	NNS
cord-333636-h2sg6shp	38	20	(	(	-LRB-
cord-333636-h2sg6shp	38	21	20	20	CD
cord-333636-h2sg6shp	38	22	µl	µl	IN
cord-333636-h2sg6shp	38	23	final	final	JJ
cord-333636-h2sg6shp	38	24	volume	volume	NN
cord-333636-h2sg6shp	38	25	)	)	-RRB-
cord-333636-h2sg6shp	38	26	.	.	.
cord-333636-h2sg6shp	39	1	Synthesized	synthesize	VBN
cord-333636-h2sg6shp	39	2	RNAs	rna	NNS
cord-333636-h2sg6shp	39	3	were	be	VBD
cord-333636-h2sg6shp	39	4	purified	purify	VBN
cord-333636-h2sg6shp	39	5	using	use	VBG
cord-333636-h2sg6shp	39	6	MicroSpin	MicroSpin	NNP
cord-333636-h2sg6shp	39	7	columns	column	NNS
cord-333636-h2sg6shp	39	8	S-200HR	S-200HR	NNP
cord-333636-h2sg6shp	39	9	(	(	-LRB-
cord-333636-h2sg6shp	39	10	Amersham	Amersham	NNP
cord-333636-h2sg6shp	39	11	Pharmacia	Pharmacia	NNP
cord-333636-h2sg6shp	39	12	Biotech	Biotech	NNP
cord-333636-h2sg6shp	39	13	)	)	-RRB-
cord-333636-h2sg6shp	39	14	.	.	.
cord-333636-h2sg6shp	40	1	Two	two	CD
cord-333636-h2sg6shp	40	2	IBDV	IBDV	NNP
cord-333636-h2sg6shp	40	3	virus	virus	NN
cord-333636-h2sg6shp	40	4	-	-	HYPH
cord-333636-h2sg6shp	40	5	derived	derive	VBN
cord-333636-h2sg6shp	40	6	ssRNA	ssrna	NN
cord-333636-h2sg6shp	40	7	probes	probe	NNS
cord-333636-h2sg6shp	40	8	were	be	VBD
cord-333636-h2sg6shp	40	9	produced	produce	VBN
cord-333636-h2sg6shp	40	10	,	,	,
cord-333636-h2sg6shp	40	11	probes	probe	NNS
cord-333636-h2sg6shp	40	12	A	A	NNP
cord-333636-h2sg6shp	40	13	and	and	CC
cord-333636-h2sg6shp	40	14	B.	B.	NNP
cord-333636-h2sg6shp	40	15	RNA	RNA	NNP
cord-333636-h2sg6shp	40	16	probe	probe	VB
cord-333636-h2sg6shp	40	17	A	a	NN
cord-333636-h2sg6shp	41	1	(	(	-LRB-
cord-333636-h2sg6shp	41	2	3040	3040	CD
cord-333636-h2sg6shp	41	3	nt	nt	NNP
cord-333636-h2sg6shp	41	4	)	)	-RRB-
cord-333636-h2sg6shp	41	5	was	be	VBD
cord-333636-h2sg6shp	41	6	synthesized	synthesize	VBN
cord-333636-h2sg6shp	41	7	from	from	IN
cord-333636-h2sg6shp	41	8	IBDV	IBDV	NNP
cord-333636-h2sg6shp	41	9	ORF	ORF	NNP
cord-333636-h2sg6shp	41	10	A2	A2	NNP
cord-333636-h2sg6shp	41	11	by	by	IN
cord-333636-h2sg6shp	41	12	transcription	transcription	NN
cord-333636-h2sg6shp	41	13	from	from	IN
cord-333636-h2sg6shp	41	14	the	the	DT
cord-333636-h2sg6shp	41	15	plasmid	plasmid	NN
cord-333636-h2sg6shp	41	16	pcDNA	pcdna	RB
cord-333636-h2sg6shp	41	17	-	-	:
cord-333636-h2sg6shp	41	18	POLY	POLY	NNP
cord-333636-h2sg6shp	41	19	[	[	-LRB-
cord-333636-h2sg6shp	41	20	40	40	CD
cord-333636-h2sg6shp	41	21	]	]	-RRB-
cord-333636-h2sg6shp	41	22	linearized	linearize	VBN
cord-333636-h2sg6shp	41	23	with	with	IN
cord-333636-h2sg6shp	41	24	SmaI.	smai.	NN
cord-333636-h2sg6shp	42	1	The	the	DT
cord-333636-h2sg6shp	42	2	sequence	sequence	NN
cord-333636-h2sg6shp	42	3	of	of	IN
cord-333636-h2sg6shp	42	4	ssRNA	ssRNA	``
cord-333636-h2sg6shp	42	5	probe	probe	NN
cord-333636-h2sg6shp	42	6	A	A	NNP
cord-333636-h2sg6shp	42	7	corresponded	correspond	VBD
cord-333636-h2sg6shp	42	8	to	to	IN
cord-333636-h2sg6shp	42	9	IBDV	IBDV	NNP
cord-333636-h2sg6shp	42	10	polyprotein	polyprotein	NNP
cord-333636-h2sg6shp	42	11	coding	code	VBG
cord-333636-h2sg6shp	42	12	region	region	NN
cord-333636-h2sg6shp	42	13	.	.	.
cord-333636-h2sg6shp	43	1	RNA	RNA	NNP
cord-333636-h2sg6shp	43	2	probe	probe	NN
cord-333636-h2sg6shp	43	3	B	b	NN
cord-333636-h2sg6shp	43	4	(	(	-LRB-
cord-333636-h2sg6shp	43	5	186	186	CD
cord-333636-h2sg6shp	43	6	nt	nt	NN
cord-333636-h2sg6shp	43	7	)	)	-RRB-
cord-333636-h2sg6shp	43	8	contained	contain	VBD
cord-333636-h2sg6shp	43	9	the	the	DT
cord-333636-h2sg6shp	43	10	5	5	CD
cord-333636-h2sg6shp	43	11	first	first	JJ
cord-333636-h2sg6shp	43	12	152	152	CD
cord-333636-h2sg6shp	43	13	nt	nt	RB
cord-333636-h2sg6shp	43	14	fused	fuse	VBN
cord-333636-h2sg6shp	43	15	to	to	IN
cord-333636-h2sg6shp	43	16	the	the	DT
cord-333636-h2sg6shp	43	17	3	3	CD
cord-333636-h2sg6shp	43	18	34	34	CD
cord-333636-h2sg6shp	43	19	nt	nt	RB
cord-333636-h2sg6shp	43	20	non	non	JJ
cord-333636-h2sg6shp	43	21	-	-	JJ
cord-333636-h2sg6shp	43	22	coding	coding	JJ
cord-333636-h2sg6shp	43	23	regions	region	NNS
cord-333636-h2sg6shp	43	24	(	(	-LRB-
cord-333636-h2sg6shp	43	25	UTRs	utr	NNS
cord-333636-h2sg6shp	43	26	)	)	-RRB-
cord-333636-h2sg6shp	43	27	from	from	IN
cord-333636-h2sg6shp	43	28	segment	segment	NN
cord-333636-h2sg6shp	44	1	B.	B.	NNP
cord-333636-h2sg6shp	45	1	This	this	DT
cord-333636-h2sg6shp	45	2	probe	probe	NN
cord-333636-h2sg6shp	45	3	was	be	VBD
cord-333636-h2sg6shp	45	4	produced	produce	VBN
cord-333636-h2sg6shp	45	5	using	use	VBG
cord-333636-h2sg6shp	45	6	the	the	DT
cord-333636-h2sg6shp	45	7	plasmid	plasmid	NN
cord-333636-h2sg6shp	45	8	TV2	TV2	NNP
cord-333636-h2sg6shp	45	9	-	-	HYPH
cord-333636-h2sg6shp	45	10	5	5	CD
cord-333636-h2sg6shp	45	11	3	3	CD
cord-333636-h2sg6shp	46	1	(	(	-LRB-
cord-333636-h2sg6shp	46	2	Maraver	Maraver	NNP
cord-333636-h2sg6shp	46	3	et	et	NNP
cord-333636-h2sg6shp	46	4	al	al	NNP
cord-333636-h2sg6shp	46	5	.	.	.
cord-333636-h2sg6shp	47	1	,	,	,
cord-333636-h2sg6shp	47	2	submitted	submit	VBN
cord-333636-h2sg6shp	47	3	for	for	IN
cord-333636-h2sg6shp	47	4	publication	publication	NN
cord-333636-h2sg6shp	47	5	)	)	-RRB-
cord-333636-h2sg6shp	47	6	linearized	linearize	VBN
cord-333636-h2sg6shp	47	7	with	with	IN
cord-333636-h2sg6shp	47	8	SmaI.	SmaI.	NNP
cord-333636-h2sg6shp	47	9	Two	two	CD
cord-333636-h2sg6shp	47	10	non	non	JJ
cord-333636-h2sg6shp	47	11	-	-	JJ
cord-333636-h2sg6shp	47	12	viral	viral	JJ
cord-333636-h2sg6shp	47	13	ssRNA	ssrna	NN
cord-333636-h2sg6shp	47	14	probes	probe	NNS
cord-333636-h2sg6shp	47	15	,	,	,
cord-333636-h2sg6shp	47	16	GFP	GFP	NNP
cord-333636-h2sg6shp	47	17	and	and	CC
cord-333636-h2sg6shp	47	18	PL	PL	NNP
cord-333636-h2sg6shp	47	19	,	,	,
cord-333636-h2sg6shp	47	20	were	be	VBD
cord-333636-h2sg6shp	47	21	also	also	RB
cord-333636-h2sg6shp	47	22	produced	produce	VBN
cord-333636-h2sg6shp	47	23	as	as	IN
cord-333636-h2sg6shp	47	24	described	describe	VBN
cord-333636-h2sg6shp	47	25	above	above	RB
cord-333636-h2sg6shp	47	26	.	.	.
cord-333636-h2sg6shp	48	1	RNA	RNA	NNP
cord-333636-h2sg6shp	48	2	probe	probe	NN
cord-333636-h2sg6shp	48	3	GFP	GFP	NNP
cord-333636-h2sg6shp	48	4	(	(	-LRB-
cord-333636-h2sg6shp	48	5	1300	1300	CD
cord-333636-h2sg6shp	48	6	nt	nt	RB
cord-333636-h2sg6shp	48	7	)	)	-RRB-
cord-333636-h2sg6shp	48	8	was	be	VBD
cord-333636-h2sg6shp	48	9	obtained	obtain	VBN
cord-333636-h2sg6shp	48	10	from	from	IN
cord-333636-h2sg6shp	48	11	green	green	JJ
cord-333636-h2sg6shp	48	12	fluorescent	fluorescent	NN
cord-333636-h2sg6shp	48	13	protein	protein	NN
cord-333636-h2sg6shp	48	14	(	(	-LRB-
cord-333636-h2sg6shp	49	1	GFP	GFP	NNP
cord-333636-h2sg6shp	49	2	)	)	-RRB-
cord-333636-h2sg6shp	49	3	mRNA	mrna	NN
cord-333636-h2sg6shp	49	4	fused	fuse	VBD
cord-333636-h2sg6shp	49	5	to	to	IN
cord-333636-h2sg6shp	49	6	the	the	DT
cord-333636-h2sg6shp	49	7	encephalomyocarditis	encephalomyocarditis	NNP
cord-333636-h2sg6shp	49	8	virus	virus	NN
cord-333636-h2sg6shp	49	9	(	(	-LRB-
cord-333636-h2sg6shp	49	10	EMCV	EMCV	NNP
cord-333636-h2sg6shp	49	11	)	)	-RRB-
cord-333636-h2sg6shp	49	12	leader	leader	NN
cord-333636-h2sg6shp	49	13	sequence	sequence	NN
cord-333636-h2sg6shp	49	14	.	.	.
cord-333636-h2sg6shp	50	1	This	this	DT
cord-333636-h2sg6shp	50	2	probe	probe	NN
cord-333636-h2sg6shp	50	3	was	be	VBD
cord-333636-h2sg6shp	50	4	produced	produce	VBN
cord-333636-h2sg6shp	50	5	by	by	IN
cord-333636-h2sg6shp	50	6	in	in	FW
cord-333636-h2sg6shp	50	7	vitro	vitro	FW
cord-333636-h2sg6shp	50	8	transcription	transcription	NN
cord-333636-h2sg6shp	50	9	using	use	VBG
cord-333636-h2sg6shp	50	10	as	as	IN
cord-333636-h2sg6shp	50	11	a	a	DT
cord-333636-h2sg6shp	50	12	template	template	NN
cord-333636-h2sg6shp	50	13	the	the	DT
cord-333636-h2sg6shp	50	14	pVOTE2-GFP	pvote2-gfp	CD
cord-333636-h2sg6shp	50	15	plasmid	plasmid	NN
cord-333636-h2sg6shp	50	16	restricted	restrict	VBN
cord-333636-h2sg6shp	50	17	with	with	IN
cord-333636-h2sg6shp	50	18	PstI	PstI	NNP
cord-333636-h2sg6shp	50	19	and	and	CC
cord-333636-h2sg6shp	50	20	Klenow	Klenow	NNP
cord-333636-h2sg6shp	50	21	-	-	HYPH
cord-333636-h2sg6shp	50	22	treated	treat	VBN
cord-333636-h2sg6shp	50	23	.	.	.
cord-333636-h2sg6shp	51	1	Plasmid	Plasmid	NNP
cord-333636-h2sg6shp	51	2	pVOTE2-GFP	pVOTE2-GFP	NNP
cord-333636-h2sg6shp	51	3	was	be	VBD
cord-333636-h2sg6shp	51	4	obtained	obtain	VBN
cord-333636-h2sg6shp	51	5	by	by	IN
cord-333636-h2sg6shp	51	6	blunt	blunt	JJ
cord-333636-h2sg6shp	51	7	-	-	HYPH
cord-333636-h2sg6shp	51	8	end	end	NN
cord-333636-h2sg6shp	51	9	ligation	ligation	NN
cord-333636-h2sg6shp	51	10	of	of	IN
cord-333636-h2sg6shp	51	11	plasmid	plasmid	NN
cord-333636-h2sg6shp	51	12	pVOTE-1	pVOTE-1	NNP
cord-333636-h2sg6shp	51	13	[	[	-LRB-
cord-333636-h2sg6shp	51	14	44	44	CD
cord-333636-h2sg6shp	51	15	]	]	-RRB-
cord-333636-h2sg6shp	51	16	restricted	restrict	VBN
cord-333636-h2sg6shp	51	17	with	with	IN
cord-333636-h2sg6shp	51	18	DraI	DraI	NNP
cord-333636-h2sg6shp	51	19	and	and	CC
cord-333636-h2sg6shp	51	20	SmaI	SmaI	NNP
cord-333636-h2sg6shp	51	21	,	,	,
cord-333636-h2sg6shp	51	22	and	and	CC
cord-333636-h2sg6shp	51	23	the	the	DT
cord-333636-h2sg6shp	51	24	GFP	GFP	NNP
cord-333636-h2sg6shp	51	25	gene	gene	NN
cord-333636-h2sg6shp	51	26	excised	excise	VBN
cord-333636-h2sg6shp	51	27	by	by	IN
cord-333636-h2sg6shp	51	28	NotI	NotI	NNP
cord-333636-h2sg6shp	51	29	digestion	digestion	NN
cord-333636-h2sg6shp	51	30	from	from	IN
cord-333636-h2sg6shp	51	31	the	the	DT
cord-333636-h2sg6shp	51	32	Green	Green	NNP
cord-333636-h2sg6shp	51	33	Lantern	Lantern	NNP
cord-333636-h2sg6shp	51	34	plasmid	plasmid	NN
cord-333636-h2sg6shp	51	35	(	(	-LRB-
cord-333636-h2sg6shp	51	36	Gibco	Gibco	NNP
cord-333636-h2sg6shp	51	37	BRL	BRL	NNP
cord-333636-h2sg6shp	51	38	)	)	-RRB-
cord-333636-h2sg6shp	51	39	followed	follow	VBN
cord-333636-h2sg6shp	51	40	by	by	IN
cord-333636-h2sg6shp	51	41	treatment	treatment	NN
cord-333636-h2sg6shp	51	42	with	with	IN
cord-333636-h2sg6shp	51	43	Klenow	Klenow	NNP
cord-333636-h2sg6shp	51	44	.	.	.
cord-333636-h2sg6shp	52	1	RNA	RNA	NNP
cord-333636-h2sg6shp	52	2	probe	probe	NN
cord-333636-h2sg6shp	52	3	PL	PL	NNP
cord-333636-h2sg6shp	52	4	(	(	-LRB-
cord-333636-h2sg6shp	52	5	100	100	CD
cord-333636-h2sg6shp	52	6	nt	nt	JJ
cord-333636-h2sg6shp	52	7	)	)	-RRB-
cord-333636-h2sg6shp	52	8	,	,	,
cord-333636-h2sg6shp	52	9	was	be	VBD
cord-333636-h2sg6shp	52	10	obtained	obtain	VBN
cord-333636-h2sg6shp	52	11	by	by	IN
cord-333636-h2sg6shp	52	12	run	run	VBN
cord-333636-h2sg6shp	52	13	off	off	RP
cord-333636-h2sg6shp	52	14	transcription	transcription	NN
cord-333636-h2sg6shp	52	15	of	of	IN
cord-333636-h2sg6shp	52	16	the	the	DT
cord-333636-h2sg6shp	52	17	polylinker	polylinker	NNP
cord-333636-h2sg6shp	52	18	region	region	NN
cord-333636-h2sg6shp	52	19	of	of	IN
cord-333636-h2sg6shp	52	20	the	the	DT
cord-333636-h2sg6shp	52	21	pcDNA3	pcDNA3	NNP
cord-333636-h2sg6shp	52	22	plasmid	plasmid	NN
cord-333636-h2sg6shp	52	23	(	(	-LRB-
cord-333636-h2sg6shp	52	24	Promega	Promega	NNP
cord-333636-h2sg6shp	52	25	)	)	-RRB-
cord-333636-h2sg6shp	52	26	linearized	linearize	VBN
cord-333636-h2sg6shp	52	27	with	with	IN
cord-333636-h2sg6shp	52	28	XbaI.	xbai.	NN
cord-333636-h2sg6shp	53	1	The	the	DT
cord-333636-h2sg6shp	53	2	purity	purity	NN
cord-333636-h2sg6shp	53	3	of	of	IN
cord-333636-h2sg6shp	53	4	all	all	DT
cord-333636-h2sg6shp	53	5	synthesized	synthesize	VBN
cord-333636-h2sg6shp	53	6	ssRNA	ssrna	NN
cord-333636-h2sg6shp	53	7	probes	probe	NNS
cord-333636-h2sg6shp	53	8	was	be	VBD
cord-333636-h2sg6shp	53	9	confirmed	confirm	VBN
cord-333636-h2sg6shp	53	10	by	by	IN
cord-333636-h2sg6shp	53	11	polyacrylamide	polyacrylamide	NN
cord-333636-h2sg6shp	53	12	gel	gel	NN
cord-333636-h2sg6shp	53	13	electrophoresis	electrophoresis	NN
cord-333636-h2sg6shp	53	14	under	under	IN
cord-333636-h2sg6shp	53	15	denaturing	denaturing	JJ
cord-333636-h2sg6shp	53	16	conditions	condition	NNS
cord-333636-h2sg6shp	53	17	and	and	CC
cord-333636-h2sg6shp	53	18	detection	detection	NN
cord-333636-h2sg6shp	53	19	by	by	IN
cord-333636-h2sg6shp	53	20	autoradiography	autoradiography	NNP
cord-333636-h2sg6shp	53	21	.	.	.
cord-333636-h2sg6shp	54	1	The	the	DT
cord-333636-h2sg6shp	54	2	concentration	concentration	NN
cord-333636-h2sg6shp	54	3	of	of	IN
cord-333636-h2sg6shp	54	4	RNA	RNA	NNP
cord-333636-h2sg6shp	54	5	preparations	preparation	NNS
cord-333636-h2sg6shp	54	6	was	be	VBD
cord-333636-h2sg6shp	54	7	estimated	estimate	VBN
cord-333636-h2sg6shp	54	8	by	by	IN
cord-333636-h2sg6shp	54	9	either	either	DT
cord-333636-h2sg6shp	54	10	electrophoresis	electrophoresis	NN
cord-333636-h2sg6shp	54	11	in	in	IN
cord-333636-h2sg6shp	54	12	polyacrylamide	polyacrylamide	NN
cord-333636-h2sg6shp	54	13	or	or	CC
cord-333636-h2sg6shp	54	14	agarose	agarose	JJ
cord-333636-h2sg6shp	54	15	gels	gel	NNS
cord-333636-h2sg6shp	54	16	and	and	CC
cord-333636-h2sg6shp	54	17	ethidium	ethidium	NN
cord-333636-h2sg6shp	54	18	bromide	bromide	NN
cord-333636-h2sg6shp	54	19	staining	staining	NN
cord-333636-h2sg6shp	54	20	or	or	CC
cord-333636-h2sg6shp	54	21	726	726	CD
cord-333636-h2sg6shp	54	22	G.	G.	NNP
cord-333636-h2sg6shp	54	23	Kochan	Kochan	NNP
cord-333636-h2sg6shp	54	24	et	et	NNP
cord-333636-h2sg6shp	54	25	al	al	NNP
cord-333636-h2sg6shp	54	26	.	.	.
cord-333636-h2sg6shp	55	1	For	for	IN
cord-333636-h2sg6shp	55	2	binding	bind	VBG
cord-333636-h2sg6shp	55	3	studies	study	NNS
cord-333636-h2sg6shp	55	4	to	to	TO
cord-333636-h2sg6shp	55	5	double	double	RB
cord-333636-h2sg6shp	55	6	-	-	HYPH
cord-333636-h2sg6shp	55	7	stranded	strand	VBN
cord-333636-h2sg6shp	55	8	IBDV	IBDV	NNP
cord-333636-h2sg6shp	55	9	RNAs	rna	NNS
cord-333636-h2sg6shp	55	10	(	(	-LRB-
cord-333636-h2sg6shp	55	11	dsRNAs	dsRNAs	NNP
cord-333636-h2sg6shp	55	12	)	)	-RRB-
cord-333636-h2sg6shp	55	13	,	,	,
cord-333636-h2sg6shp	55	14	virus	virus	NN
cord-333636-h2sg6shp	55	15	dsRNA	dsrna	NN
cord-333636-h2sg6shp	55	16	was	be	VBD
cord-333636-h2sg6shp	55	17	purified	purify	VBN
cord-333636-h2sg6shp	55	18	from	from	IN
cord-333636-h2sg6shp	55	19	BSC40	BSC40	NNP
cord-333636-h2sg6shp	55	20	cells	cell	NNS
cord-333636-h2sg6shp	55	21	infected	infect	VBN
cord-333636-h2sg6shp	55	22	with	with	IN
cord-333636-h2sg6shp	55	23	IBDV	IBDV	NNP
cord-333636-h2sg6shp	55	24	using	use	VBG
cord-333636-h2sg6shp	55	25	the	the	DT
cord-333636-h2sg6shp	55	26	Ultraspect	Ultraspect	NNP
cord-333636-h2sg6shp	55	27	RNA	RNA	NNP
cord-333636-h2sg6shp	55	28	extraction	extraction	NN
cord-333636-h2sg6shp	55	29	reagent	reagent	NN
cord-333636-h2sg6shp	55	30	kit	kit	NN
cord-333636-h2sg6shp	55	31	(	(	-LRB-
cord-333636-h2sg6shp	55	32	Biotecx	Biotecx	NNP
cord-333636-h2sg6shp	55	33	laboratories	laboratory	NNS
cord-333636-h2sg6shp	55	34	,	,	,
cord-333636-h2sg6shp	55	35	Inc.	Inc.	NNP
cord-333636-h2sg6shp	55	36	)	)	-RRB-
cord-333636-h2sg6shp	55	37	following	follow	VBG
cord-333636-h2sg6shp	55	38	the	the	DT
cord-333636-h2sg6shp	55	39	instructions	instruction	NNS
cord-333636-h2sg6shp	55	40	provided	provide	VBN
cord-333636-h2sg6shp	55	41	by	by	IN
cord-333636-h2sg6shp	55	42	the	the	DT
cord-333636-h2sg6shp	55	43	manufacturer	manufacturer	NN
cord-333636-h2sg6shp	55	44	.	.	.
cord-333636-h2sg6shp	56	1	Subsequently	subsequently	RB
cord-333636-h2sg6shp	56	2	,	,	,
cord-333636-h2sg6shp	56	3	IBDV	IBDV	NNP
cord-333636-h2sg6shp	56	4	dsRNA	dsrna	NN
cord-333636-h2sg6shp	57	1	segments	segment	NNS
cord-333636-h2sg6shp	57	2	A	A	NNP
cord-333636-h2sg6shp	57	3	and	and	CC
cord-333636-h2sg6shp	57	4	B	B	NNP
cord-333636-h2sg6shp	57	5	were	be	VBD
cord-333636-h2sg6shp	57	6	separated	separate	VBN
cord-333636-h2sg6shp	57	7	in	in	IN
cord-333636-h2sg6shp	57	8	1	1	CD
cord-333636-h2sg6shp	57	9	%	%	NN
cord-333636-h2sg6shp	57	10	agarose	agarose	JJ
cord-333636-h2sg6shp	57	11	gels	gel	NNS
cord-333636-h2sg6shp	57	12	,	,	,
cord-333636-h2sg6shp	57	13	and	and	CC
cord-333636-h2sg6shp	57	14	segment	segment	NN
cord-333636-h2sg6shp	57	15	A	A	NNP
cord-333636-h2sg6shp	57	16	was	be	VBD
cord-333636-h2sg6shp	57	17	purified	purify	VBN
cord-333636-h2sg6shp	57	18	using	use	VBG
cord-333636-h2sg6shp	57	19	RNaid	RNaid	NNP
cord-333636-h2sg6shp	57	20	kit	kit	NN
cord-333636-h2sg6shp	57	21	(	(	-LRB-
cord-333636-h2sg6shp	57	22	Q.	Q.	NNP
cord-333636-h2sg6shp	57	23	Biogene	Biogene	NNP
cord-333636-h2sg6shp	57	24	)	)	-RRB-
cord-333636-h2sg6shp	57	25	.	.	.
cord-333636-h2sg6shp	58	1	Plasmid	Plasmid	NNP
cord-333636-h2sg6shp	58	2	DNA	DNA	NNP
cord-333636-h2sg6shp	58	3	pcDNA3	pcdna3	NN
cord-333636-h2sg6shp	58	4	linearized	linearize	VBN
cord-333636-h2sg6shp	58	5	with	with	IN
cord-333636-h2sg6shp	58	6	SmaI	SmaI	NNP
cord-333636-h2sg6shp	58	7	was	be	VBD
cord-333636-h2sg6shp	58	8	used	use	VBN
cord-333636-h2sg6shp	58	9	as	as	IN
cord-333636-h2sg6shp	58	10	double	double	RB
cord-333636-h2sg6shp	58	11	-	-	HYPH
cord-333636-h2sg6shp	58	12	stranded	strand	VBN
cord-333636-h2sg6shp	58	13	DNA	dna	NN
cord-333636-h2sg6shp	58	14	(	(	-LRB-
cord-333636-h2sg6shp	58	15	dsDNA	dsdna	NN
cord-333636-h2sg6shp	58	16	)	)	-RRB-
cord-333636-h2sg6shp	58	17	competitor	competitor	NN
cord-333636-h2sg6shp	58	18	probe	probe	NN
cord-333636-h2sg6shp	58	19	.	.	.
cord-333636-h2sg6shp	59	1	Single	single	JJ
cord-333636-h2sg6shp	59	2	-	-	HYPH
cord-333636-h2sg6shp	59	3	stranded	strand	VBN
cord-333636-h2sg6shp	59	4	DNA	dna	NN
cord-333636-h2sg6shp	59	5	from	from	IN
cord-333636-h2sg6shp	59	6	PhiX	PhiX	NNP
cord-333636-h2sg6shp	59	7	phage	phage	NN
cord-333636-h2sg6shp	59	8	virus	virus	NN
cord-333636-h2sg6shp	59	9	(	(	-LRB-
cord-333636-h2sg6shp	59	10	Biolabs	Biolabs	NNP
cord-333636-h2sg6shp	59	11	)	)	-RRB-
cord-333636-h2sg6shp	59	12	was	be	VBD
cord-333636-h2sg6shp	59	13	used	use	VBN
cord-333636-h2sg6shp	59	14	as	as	IN
cord-333636-h2sg6shp	59	15	ssDNA	ssdna	JJ
cord-333636-h2sg6shp	59	16	competitor	competitor	NN
cord-333636-h2sg6shp	59	17	probe	probe	NN
cord-333636-h2sg6shp	59	18	.	.	.
cord-333636-h2sg6shp	60	1	WT	WT	NNP
cord-333636-h2sg6shp	60	2	and	and	CC
cord-333636-h2sg6shp	60	3	mutant	mutant	JJ
cord-333636-h2sg6shp	60	4	VP3	vp3	NN
cord-333636-h2sg6shp	60	5	proteins	protein	NNS
cord-333636-h2sg6shp	60	6	were	be	VBD
cord-333636-h2sg6shp	60	7	expressed	express	VBN
cord-333636-h2sg6shp	60	8	using	use	VBG
cord-333636-h2sg6shp	60	9	a	a	DT
cord-333636-h2sg6shp	60	10	baculovirus	baculovirus	NN
cord-333636-h2sg6shp	60	11	-	-	HYPH
cord-333636-h2sg6shp	60	12	based	base	VBN
cord-333636-h2sg6shp	60	13	expression	expression	NN
cord-333636-h2sg6shp	60	14	system	system	NN
cord-333636-h2sg6shp	60	15	.	.	.
cord-333636-h2sg6shp	61	1	Briefly	briefly	RB
cord-333636-h2sg6shp	61	2	,	,	,
cord-333636-h2sg6shp	61	3	a	a	DT
cord-333636-h2sg6shp	61	4	150	150	CD
cord-333636-h2sg6shp	61	5	mm	mm	NN
cord-333636-h2sg6shp	61	6	diameter	diameter	NN
cord-333636-h2sg6shp	61	7	culture	culture	NN
cord-333636-h2sg6shp	61	8	plate	plate	NN
cord-333636-h2sg6shp	61	9	of	of	IN
cord-333636-h2sg6shp	61	10	confluent	confluent	JJ
cord-333636-h2sg6shp	61	11	H5	H5	NNP
cord-333636-h2sg6shp	61	12	insect	insect	NN
cord-333636-h2sg6shp	61	13	cells	cell	NNS
cord-333636-h2sg6shp	61	14	were	be	VBD
cord-333636-h2sg6shp	61	15	infected	infect	VBN
cord-333636-h2sg6shp	61	16	with	with	IN
cord-333636-h2sg6shp	61	17	rBVs	rbv	NNS
cord-333636-h2sg6shp	61	18	at	at	IN
cord-333636-h2sg6shp	61	19	a	a	DT
cord-333636-h2sg6shp	61	20	multiplicity	multiplicity	NN
cord-333636-h2sg6shp	61	21	of	of	IN
cord-333636-h2sg6shp	61	22	infection	infection	NN
cord-333636-h2sg6shp	61	23	(	(	-LRB-
cord-333636-h2sg6shp	61	24	moi	moi	NNP
cord-333636-h2sg6shp	61	25	)	)	-RRB-
cord-333636-h2sg6shp	61	26	of	of	IN
cord-333636-h2sg6shp	61	27	1	1	CD
cord-333636-h2sg6shp	61	28	.	.	.
cord-333636-h2sg6shp	62	1	Infected	infected	JJ
cord-333636-h2sg6shp	62	2	cells	cell	NNS
cord-333636-h2sg6shp	62	3	were	be	VBD
cord-333636-h2sg6shp	62	4	harvested	harvest	VBN
cord-333636-h2sg6shp	62	5	at	at	IN
cord-333636-h2sg6shp	62	6	72	72	CD
cord-333636-h2sg6shp	62	7	hpi	hpi	NNS
cord-333636-h2sg6shp	62	8	and	and	CC
cord-333636-h2sg6shp	62	9	lysed	lyse	VBN
cord-333636-h2sg6shp	62	10	on	on	IN
cord-333636-h2sg6shp	62	11	ice	ice	NN
cord-333636-h2sg6shp	62	12	with	with	IN
cord-333636-h2sg6shp	62	13	a	a	DT
cord-333636-h2sg6shp	62	14	Dounce	Dounce	NNP
cord-333636-h2sg6shp	62	15	homogenizer	homogenizer	NN
cord-333636-h2sg6shp	62	16	in	in	IN
cord-333636-h2sg6shp	62	17	TNN	TNN	NNP
cord-333636-h2sg6shp	62	18	buffer	buffer	NN
cord-333636-h2sg6shp	62	19	(	(	-LRB-
cord-333636-h2sg6shp	62	20	50	50	CD
cord-333636-h2sg6shp	62	21	mM	mm	CD
cord-333636-h2sg6shp	62	22	Tris	Tris	NNP
cord-333636-h2sg6shp	62	23	,	,	,
cord-333636-h2sg6shp	62	24	500	500	CD
cord-333636-h2sg6shp	62	25	mM	mm	CD
cord-333636-h2sg6shp	62	26	NaCl	nacl	NN
cord-333636-h2sg6shp	62	27	,	,	,
cord-333636-h2sg6shp	62	28	and	and	CC
cord-333636-h2sg6shp	62	29	0.1	0.1	CD
cord-333636-h2sg6shp	62	30	%	%	NN
cord-333636-h2sg6shp	62	31	NP-40	NP-40	NNP
cord-333636-h2sg6shp	62	32	,	,	,
cord-333636-h2sg6shp	62	33	pH	pH	NNP
cord-333636-h2sg6shp	62	34	7.5	7.5	CD
cord-333636-h2sg6shp	62	35	)	)	-RRB-
cord-333636-h2sg6shp	62	36	in	in	IN
cord-333636-h2sg6shp	62	37	the	the	DT
cord-333636-h2sg6shp	62	38	presence	presence	NN
cord-333636-h2sg6shp	62	39	of	of	IN
cord-333636-h2sg6shp	62	40	protease	protease	NN
cord-333636-h2sg6shp	62	41	inhibitors	inhibitor	NNS
cord-333636-h2sg6shp	62	42	(	(	-LRB-
cord-333636-h2sg6shp	62	43	Complete	Complete	NNP
cord-333636-h2sg6shp	62	44	Mini	Mini	NNP
cord-333636-h2sg6shp	62	45	,	,	,
cord-333636-h2sg6shp	62	46	Roche	Roche	NNP
cord-333636-h2sg6shp	62	47	)	)	-RRB-
cord-333636-h2sg6shp	62	48	.	.	.
cord-333636-h2sg6shp	63	1	Cell	cell	NN
cord-333636-h2sg6shp	63	2	lysates	lysate	NNS
cord-333636-h2sg6shp	63	3	were	be	VBD
cord-333636-h2sg6shp	63	4	clarified	clarify	VBN
cord-333636-h2sg6shp	63	5	by	by	IN
cord-333636-h2sg6shp	63	6	centrifugation	centrifugation	NN
cord-333636-h2sg6shp	63	7	at	at	IN
cord-333636-h2sg6shp	63	8	1000	1000	CD
cord-333636-h2sg6shp	63	9	X	x	NN
cord-333636-h2sg6shp	63	10	g	g	NN
cord-333636-h2sg6shp	63	11	for	for	IN
cord-333636-h2sg6shp	63	12	10	10	CD
cord-333636-h2sg6shp	63	13	min	min	NN
cord-333636-h2sg6shp	63	14	at	at	IN
cord-333636-h2sg6shp	63	15	4	4	CD
cord-333636-h2sg6shp	63	16	•	•	NN
cord-333636-h2sg6shp	64	1	C.	C.	NNP
cord-333636-h2sg6shp	64	2	Recombinant	Recombinant	NNP
cord-333636-h2sg6shp	64	3	proteins	protein	NNS
cord-333636-h2sg6shp	64	4	were	be	VBD
cord-333636-h2sg6shp	64	5	purified	purify	VBN
cord-333636-h2sg6shp	64	6	from	from	IN
cord-333636-h2sg6shp	64	7	the	the	DT
cord-333636-h2sg6shp	64	8	resulting	result	VBG
cord-333636-h2sg6shp	64	9	supernatants	supernatant	NNS
cord-333636-h2sg6shp	64	10	using	use	VBG
cord-333636-h2sg6shp	64	11	a	a	DT
cord-333636-h2sg6shp	64	12	Ni	Ni	NNP
cord-333636-h2sg6shp	64	13	-	-	HYPH
cord-333636-h2sg6shp	64	14	His	-PRON-	PRP$
cord-333636-h2sg6shp	64	15	trap	trap	NN
cord-333636-h2sg6shp	64	16	affinity	affinity	NN
cord-333636-h2sg6shp	64	17	column	column	NN
cord-333636-h2sg6shp	64	18	(	(	-LRB-
cord-333636-h2sg6shp	64	19	Pharmacia	Pharmacia	NNP
cord-333636-h2sg6shp	64	20	LKB	LKB	NNP
cord-333636-h2sg6shp	64	21	)	)	-RRB-
cord-333636-h2sg6shp	64	22	following	follow	VBG
cord-333636-h2sg6shp	64	23	the	the	DT
cord-333636-h2sg6shp	64	24	instructions	instruction	NNS
cord-333636-h2sg6shp	64	25	provided	provide	VBN
cord-333636-h2sg6shp	64	26	by	by	IN
cord-333636-h2sg6shp	64	27	the	the	DT
cord-333636-h2sg6shp	64	28	manufacturers	manufacturer	NNS
cord-333636-h2sg6shp	64	29	.	.	.
cord-333636-h2sg6shp	65	1	The	the	DT
cord-333636-h2sg6shp	65	2	expressed	express	VBN
cord-333636-h2sg6shp	65	3	proteins	protein	NNS
cord-333636-h2sg6shp	65	4	were	be	VBD
cord-333636-h2sg6shp	65	5	eluted	elute	VBN
cord-333636-h2sg6shp	65	6	from	from	IN
cord-333636-h2sg6shp	65	7	the	the	DT
cord-333636-h2sg6shp	65	8	column	column	NN
cord-333636-h2sg6shp	65	9	with	with	IN
cord-333636-h2sg6shp	65	10	a	a	DT
cord-333636-h2sg6shp	65	11	continuous	continuous	JJ
cord-333636-h2sg6shp	65	12	imidazol	imidazol	JJ
cord-333636-h2sg6shp	65	13	gradient	gradient	NN
cord-333636-h2sg6shp	65	14	from	from	IN
cord-333636-h2sg6shp	65	15	4	4	CD
cord-333636-h2sg6shp	65	16	mM	mm	CD
cord-333636-h2sg6shp	65	17	to	to	IN
cord-333636-h2sg6shp	65	18	600	600	CD
cord-333636-h2sg6shp	65	19	mM.	mm.	NN
cord-333636-h2sg6shp	66	1	His	-PRON-	PRP$
cord-333636-h2sg6shp	66	2	-	-	HYPH
cord-333636-h2sg6shp	66	3	tagged	tagged	NNP
cord-333636-h2sg6shp	66	4	WT	WT	NNP
cord-333636-h2sg6shp	66	5	VP3	VP3	NNP
cord-333636-h2sg6shp	66	6	protein	protein	NN
cord-333636-h2sg6shp	66	7	was	be	VBD
cord-333636-h2sg6shp	66	8	eluted	elute	VBN
cord-333636-h2sg6shp	66	9	at	at	IN
cord-333636-h2sg6shp	66	10	an	an	DT
cord-333636-h2sg6shp	66	11	imidazol	imidazol	JJ
cord-333636-h2sg6shp	66	12	concentration	concentration	NN
cord-333636-h2sg6shp	66	13	of	of	IN
cord-333636-h2sg6shp	66	14	500	500	CD
cord-333636-h2sg6shp	66	15	mM.	mM.	NNS
cord-333636-h2sg6shp	67	1	For	for	IN
cord-333636-h2sg6shp	67	2	elution	elution	NN
cord-333636-h2sg6shp	67	3	of	of	IN
cord-333636-h2sg6shp	67	4	each	each	DT
cord-333636-h2sg6shp	67	5	VP3	VP3	NNP
cord-333636-h2sg6shp	67	6	deletion	deletion	NN
cord-333636-h2sg6shp	67	7	mutant	mutant	NN
cord-333636-h2sg6shp	67	8	,	,	,
cord-333636-h2sg6shp	67	9	imidazol	imidazol	JJ
cord-333636-h2sg6shp	67	10	concentrations	concentration	NNS
cord-333636-h2sg6shp	67	11	were	be	VBD
cord-333636-h2sg6shp	67	12	optimized	optimize	VBN
cord-333636-h2sg6shp	67	13	.	.	.
cord-333636-h2sg6shp	68	1	The	the	DT
cord-333636-h2sg6shp	68	2	purity	purity	NN
cord-333636-h2sg6shp	68	3	of	of	IN
cord-333636-h2sg6shp	68	4	eluted	elute	VBN
cord-333636-h2sg6shp	68	5	proteins	protein	NNS
cord-333636-h2sg6shp	68	6	was	be	VBD
cord-333636-h2sg6shp	68	7	assessed	assess	VBN
cord-333636-h2sg6shp	68	8	by	by	IN
cord-333636-h2sg6shp	68	9	SDS	SDS	NNP
cord-333636-h2sg6shp	68	10	-	-	HYPH
cord-333636-h2sg6shp	68	11	PAGE	PAGE	NNP
cord-333636-h2sg6shp	68	12	analysis	analysis	NN
cord-333636-h2sg6shp	68	13	and	and	CC
cord-333636-h2sg6shp	68	14	Coomassie	Coomassie	NNP
cord-333636-h2sg6shp	68	15	staining	staining	NN
cord-333636-h2sg6shp	68	16	.	.	.
cord-333636-h2sg6shp	69	1	The	the	DT
cord-333636-h2sg6shp	69	2	concentration	concentration	NN
cord-333636-h2sg6shp	69	3	of	of	IN
cord-333636-h2sg6shp	69	4	purified	purify	VBN
cord-333636-h2sg6shp	69	5	proteins	protein	NNS
cord-333636-h2sg6shp	69	6	was	be	VBD
cord-333636-h2sg6shp	69	7	estimated	estimate	VBN
cord-333636-h2sg6shp	69	8	using	use	VBG
cord-333636-h2sg6shp	69	9	the	the	DT
cord-333636-h2sg6shp	69	10	BCA	BCA	NNP
cord-333636-h2sg6shp	69	11	system	system	NN
cord-333636-h2sg6shp	69	12	(	(	-LRB-
cord-333636-h2sg6shp	69	13	Pierce	Pierce	NNP
cord-333636-h2sg6shp	69	14	)	)	-RRB-
cord-333636-h2sg6shp	69	15	.	.	.
cord-333636-h2sg6shp	70	1	To	to	TO
cord-333636-h2sg6shp	70	2	confirm	confirm	VB
cord-333636-h2sg6shp	70	3	the	the	DT
cord-333636-h2sg6shp	70	4	identity	identity	NN
cord-333636-h2sg6shp	70	5	of	of	IN
cord-333636-h2sg6shp	70	6	baculovirus	baculovirus	NNP
cord-333636-h2sg6shp	70	7	-	-	HYPH
cord-333636-h2sg6shp	70	8	expressed	express	VBN
cord-333636-h2sg6shp	70	9	VP3	vp3	NN
cord-333636-h2sg6shp	70	10	proteins	protein	NNS
cord-333636-h2sg6shp	70	11	,	,	,
cord-333636-h2sg6shp	70	12	Western	western	JJ
cord-333636-h2sg6shp	70	13	blot	blot	NN
cord-333636-h2sg6shp	70	14	analyses	analysis	NNS
cord-333636-h2sg6shp	70	15	were	be	VBD
cord-333636-h2sg6shp	70	16	carried	carry	VBN
cord-333636-h2sg6shp	70	17	out	out	RP
cord-333636-h2sg6shp	70	18	from	from	IN
cord-333636-h2sg6shp	70	19	infected	infected	JJ
cord-333636-h2sg6shp	70	20	cell	cell	NN
cord-333636-h2sg6shp	70	21	lysates	lysate	NNS
cord-333636-h2sg6shp	70	22	as	as	IN
cord-333636-h2sg6shp	70	23	described	describe	VBN
cord-333636-h2sg6shp	70	24	[	[	-LRB-
cord-333636-h2sg6shp	70	25	17	17	CD
cord-333636-h2sg6shp	70	26	]	]	-RRB-
cord-333636-h2sg6shp	70	27	.	.	.
cord-333636-h2sg6shp	71	1	Briefly	briefly	RB
cord-333636-h2sg6shp	71	2	,	,	,
cord-333636-h2sg6shp	71	3	after	after	IN
cord-333636-h2sg6shp	71	4	separation	separation	NN
cord-333636-h2sg6shp	71	5	of	of	IN
cord-333636-h2sg6shp	71	6	the	the	DT
cord-333636-h2sg6shp	71	7	cellular	cellular	JJ
cord-333636-h2sg6shp	71	8	proteins	protein	NNS
cord-333636-h2sg6shp	71	9	by	by	IN
cord-333636-h2sg6shp	71	10	SDS	SDS	NNP
cord-333636-h2sg6shp	71	11	-	-	HYPH
cord-333636-h2sg6shp	71	12	PAGE	PAGE	NNP
cord-333636-h2sg6shp	71	13	and	and	CC
cord-333636-h2sg6shp	71	14	transfer	transfer	VB
cord-333636-h2sg6shp	71	15	onto	onto	IN
cord-333636-h2sg6shp	71	16	nitrocellulose	nitrocellulose	JJ
cord-333636-h2sg6shp	71	17	membranes	membrane	NNS
cord-333636-h2sg6shp	71	18	,	,	,
cord-333636-h2sg6shp	71	19	filters	filter	NNS
cord-333636-h2sg6shp	71	20	were	be	VBD
cord-333636-h2sg6shp	71	21	saturated	saturate	VBN
cord-333636-h2sg6shp	71	22	with	with	IN
cord-333636-h2sg6shp	71	23	5	5	CD
cord-333636-h2sg6shp	71	24	%	%	NN
cord-333636-h2sg6shp	71	25	non	non	JJ
cord-333636-h2sg6shp	71	26	-	-	JJ
cord-333636-h2sg6shp	71	27	fat	fat	JJ
cord-333636-h2sg6shp	71	28	dried	dry	VBN
cord-333636-h2sg6shp	71	29	milk	milk	NN
cord-333636-h2sg6shp	71	30	in	in	IN
cord-333636-h2sg6shp	71	31	PBS	PBS	NNP
cord-333636-h2sg6shp	71	32	for	for	IN
cord-333636-h2sg6shp	71	33	1	1	CD
cord-333636-h2sg6shp	71	34	h	h	NN
cord-333636-h2sg6shp	71	35	at	at	IN
cord-333636-h2sg6shp	71	36	room	room	NN
cord-333636-h2sg6shp	71	37	temperature	temperature	NN
cord-333636-h2sg6shp	71	38	(	(	-LRB-
cord-333636-h2sg6shp	71	39	RT	RT	NNP
cord-333636-h2sg6shp	71	40	)	)	-RRB-
cord-333636-h2sg6shp	71	41	,	,	,
cord-333636-h2sg6shp	71	42	followed	follow	VBN
cord-333636-h2sg6shp	71	43	by	by	IN
cord-333636-h2sg6shp	71	44	incubation	incubation	NN
cord-333636-h2sg6shp	71	45	with	with	IN
cord-333636-h2sg6shp	71	46	specific	specific	JJ
cord-333636-h2sg6shp	71	47	anti	anti	JJ
cord-333636-h2sg6shp	71	48	-	-	JJ
cord-333636-h2sg6shp	71	49	VP3	vp3	JJ
cord-333636-h2sg6shp	71	50	monoclonal	monoclonal	NN
cord-333636-h2sg6shp	71	51	(	(	-LRB-
cord-333636-h2sg6shp	71	52	MAb	MAb	NNS
cord-333636-h2sg6shp	71	53	)	)	-RRB-
cord-333636-h2sg6shp	71	54	or	or	CC
cord-333636-h2sg6shp	71	55	polyclonal	polyclonal	JJ
cord-333636-h2sg6shp	71	56	antibodies	antibody	NNS
cord-333636-h2sg6shp	71	57	[	[	-LRB-
cord-333636-h2sg6shp	71	58	17	17	CD
cord-333636-h2sg6shp	71	59	]	]	-RRB-
cord-333636-h2sg6shp	71	60	diluted	dilute	VBN
cord-333636-h2sg6shp	71	61	1:1000	1:1000	NNS
cord-333636-h2sg6shp	71	62	in	in	IN
cord-333636-h2sg6shp	71	63	blocking	block	VBG
cord-333636-h2sg6shp	71	64	solution	solution	NN
cord-333636-h2sg6shp	71	65	.	.	.
cord-333636-h2sg6shp	72	1	Excess	excess	NN
cord-333636-h2sg6shp	72	2	of	of	IN
cord-333636-h2sg6shp	72	3	unbound	unbound	JJ
cord-333636-h2sg6shp	72	4	antibodies	antibody	NNS
cord-333636-h2sg6shp	72	5	was	be	VBD
cord-333636-h2sg6shp	72	6	removed	remove	VBN
cord-333636-h2sg6shp	72	7	by	by	IN
cord-333636-h2sg6shp	72	8	washing	wash	VBG
cord-333636-h2sg6shp	72	9	three	three	CD
cord-333636-h2sg6shp	72	10	times	time	NNS
cord-333636-h2sg6shp	72	11	in	in	IN
cord-333636-h2sg6shp	72	12	PBS	PBS	NNP
cord-333636-h2sg6shp	72	13	buffer	buffer	NN
cord-333636-h2sg6shp	72	14	.	.	.
cord-333636-h2sg6shp	73	1	After	after	IN
cord-333636-h2sg6shp	73	2	washing	washing	NN
cord-333636-h2sg6shp	73	3	,	,	,
cord-333636-h2sg6shp	73	4	horseradish	horseradish	NN
cord-333636-h2sg6shp	73	5	peroxidase	peroxidase	NN
cord-333636-h2sg6shp	73	6	-	-	HYPH
cord-333636-h2sg6shp	73	7	conjugated	conjugate	VBN
cord-333636-h2sg6shp	73	8	secondary	secondary	JJ
cord-333636-h2sg6shp	73	9	goat	goat	NN
cord-333636-h2sg6shp	73	10	-	-	HYPH
cord-333636-h2sg6shp	73	11	anti	anti	JJ
cord-333636-h2sg6shp	73	12	rabbit	rabbit	NN
cord-333636-h2sg6shp	73	13	MAb	MAb	NNS
cord-333636-h2sg6shp	73	14	or	or	CC
cord-333636-h2sg6shp	73	15	rabbit	rabbit	NN
cord-333636-h2sg6shp	73	16	anti	anti	JJ
cord-333636-h2sg6shp	73	17	-	-	JJ
cord-333636-h2sg6shp	73	18	mouse	mouse	JJ
cord-333636-h2sg6shp	73	19	MAb	MAb	NNS
cord-333636-h2sg6shp	73	20	(	(	-LRB-
cord-333636-h2sg6shp	73	21	Cappel	Cappel	NNP
cord-333636-h2sg6shp	73	22	)	)	-RRB-
cord-333636-h2sg6shp	73	23	were	be	VBD
cord-333636-h2sg6shp	73	24	added	add	VBN
cord-333636-h2sg6shp	73	25	in	in	IN
cord-333636-h2sg6shp	73	26	blocking	block	VBG
cord-333636-h2sg6shp	73	27	solution	solution	NN
cord-333636-h2sg6shp	73	28	(	(	-LRB-
cord-333636-h2sg6shp	73	29	1:1000	1:1000	CD
cord-333636-h2sg6shp	73	30	dilution	dilution	NN
cord-333636-h2sg6shp	73	31	)	)	-RRB-
cord-333636-h2sg6shp	73	32	and	and	CC
cord-333636-h2sg6shp	73	33	incubated	incubate	VBN
cord-333636-h2sg6shp	73	34	for	for	IN
cord-333636-h2sg6shp	73	35	1	1	CD
cord-333636-h2sg6shp	73	36	h	h	NN
cord-333636-h2sg6shp	73	37	at	at	IN
cord-333636-h2sg6shp	73	38	RT	RT	NNP
cord-333636-h2sg6shp	73	39	.	.	.
cord-333636-h2sg6shp	74	1	After	after	IN
cord-333636-h2sg6shp	74	2	removing	remove	VBG
cord-333636-h2sg6shp	74	3	the	the	DT
cord-333636-h2sg6shp	74	4	unbound	unbound	JJ
cord-333636-h2sg6shp	74	5	conjugates	conjugate	NNS
cord-333636-h2sg6shp	74	6	,	,	,
cord-333636-h2sg6shp	74	7	membranes	membrane	NNS
cord-333636-h2sg6shp	74	8	were	be	VBD
cord-333636-h2sg6shp	74	9	developed	develop	VBN
cord-333636-h2sg6shp	74	10	in	in	IN
cord-333636-h2sg6shp	74	11	PBS	PBS	NNP
cord-333636-h2sg6shp	74	12	containing	contain	VBG
cord-333636-h2sg6shp	74	13	0.02	0.02	CD
cord-333636-h2sg6shp	74	14	%	%	NN
cord-333636-h2sg6shp	74	15	1-chloro-4-naphtol	1-chloro-4-naphtol	CD
cord-333636-h2sg6shp	74	16	and	and	CC
cord-333636-h2sg6shp	74	17	0.006	0.006	CD
cord-333636-h2sg6shp	74	18	%	%	NN
cord-333636-h2sg6shp	74	19	hydrogen	hydrogen	NN
cord-333636-h2sg6shp	74	20	peroxide	peroxide	NN
cord-333636-h2sg6shp	74	21	.	.	.
cord-333636-h2sg6shp	75	1	After	after	IN
cord-333636-h2sg6shp	75	2	developing	develop	VBG
cord-333636-h2sg6shp	75	3	,	,	,
cord-333636-h2sg6shp	75	4	membranes	membrane	NNS
cord-333636-h2sg6shp	75	5	were	be	VBD
cord-333636-h2sg6shp	75	6	washed	wash	VBN
cord-333636-h2sg6shp	75	7	in	in	IN
cord-333636-h2sg6shp	75	8	distilled	distil	VBN
cord-333636-h2sg6shp	75	9	water	water	NN
cord-333636-h2sg6shp	75	10	and	and	CC
cord-333636-h2sg6shp	75	11	dried	dry	VBN
cord-333636-h2sg6shp	75	12	.	.	.
cord-333636-h2sg6shp	76	1	Northwestern	northwestern	JJ
cord-333636-h2sg6shp	76	2	blot	blot	NN
cord-333636-h2sg6shp	76	3	assays	assay	NNS
cord-333636-h2sg6shp	76	4	were	be	VBD
cord-333636-h2sg6shp	76	5	carried	carry	VBN
cord-333636-h2sg6shp	76	6	out	out	RP
cord-333636-h2sg6shp	76	7	with	with	IN
cord-333636-h2sg6shp	76	8	cellular	cellular	JJ
cord-333636-h2sg6shp	76	9	extracts	extract	NNS
cord-333636-h2sg6shp	76	10	from	from	IN
cord-333636-h2sg6shp	76	11	cells	cell	NNS
cord-333636-h2sg6shp	76	12	infected	infect	VBN
cord-333636-h2sg6shp	76	13	with	with	IN
cord-333636-h2sg6shp	76	14	WT	WT	NNP
cord-333636-h2sg6shp	76	15	or	or	CC
cord-333636-h2sg6shp	76	16	rBVs	rBVs	:
cord-333636-h2sg6shp	76	17	as	as	IN
cord-333636-h2sg6shp	76	18	described	describe	VBN
cord-333636-h2sg6shp	76	19	[	[	-LRB-
cord-333636-h2sg6shp	76	20	19	19	CD
cord-333636-h2sg6shp	76	21	]	]	-RRB-
cord-333636-h2sg6shp	76	22	.	.	.
cord-333636-h2sg6shp	77	1	Infected	infected	JJ
cord-333636-h2sg6shp	77	2	cells	cell	NNS
cord-333636-h2sg6shp	77	3	were	be	VBD
cord-333636-h2sg6shp	77	4	collected	collect	VBN
cord-333636-h2sg6shp	77	5	at	at	IN
cord-333636-h2sg6shp	77	6	72	72	CD
cord-333636-h2sg6shp	77	7	hpi	hpi	NNS
cord-333636-h2sg6shp	77	8	and	and	CC
cord-333636-h2sg6shp	77	9	pelleted	pellete	VBN
cord-333636-h2sg6shp	77	10	by	by	IN
cord-333636-h2sg6shp	77	11	centrifugation	centrifugation	NN
cord-333636-h2sg6shp	77	12	at	at	IN
cord-333636-h2sg6shp	77	13	400	400	CD
cord-333636-h2sg6shp	77	14	X	x	NN
cord-333636-h2sg6shp	77	15	g	g	NN
cord-333636-h2sg6shp	77	16	for	for	IN
cord-333636-h2sg6shp	77	17	5	5	CD
cord-333636-h2sg6shp	77	18	min	min	NN
cord-333636-h2sg6shp	77	19	at	at	IN
cord-333636-h2sg6shp	77	20	4	4	CD
cord-333636-h2sg6shp	77	21	•	•	NNP
cord-333636-h2sg6shp	77	22	C.	C.	NNP
cord-333636-h2sg6shp	77	23	Cell	Cell	NNP
cord-333636-h2sg6shp	77	24	pellets	pellet	NNS
cord-333636-h2sg6shp	77	25	were	be	VBD
cord-333636-h2sg6shp	77	26	washed	wash	VBN
cord-333636-h2sg6shp	77	27	with	with	IN
cord-333636-h2sg6shp	77	28	PBS	PBS	NNP
cord-333636-h2sg6shp	77	29	and	and	CC
cord-333636-h2sg6shp	77	30	lysed	lyse	VBN
cord-333636-h2sg6shp	77	31	in	in	IN
cord-333636-h2sg6shp	77	32	TNN	TNN	NNP
cord-333636-h2sg6shp	77	33	buffer	buffer	NN
cord-333636-h2sg6shp	77	34	by	by	IN
cord-333636-h2sg6shp	77	35	incubation	incubation	NN
cord-333636-h2sg6shp	77	36	on	on	IN
cord-333636-h2sg6shp	77	37	ice	ice	NN
cord-333636-h2sg6shp	77	38	for	for	IN
cord-333636-h2sg6shp	77	39	10	10	CD
cord-333636-h2sg6shp	77	40	min	min	NN
cord-333636-h2sg6shp	77	41	with	with	IN
cord-333636-h2sg6shp	77	42	frequent	frequent	JJ
cord-333636-h2sg6shp	77	43	and	and	CC
cord-333636-h2sg6shp	77	44	intensive	intensive	JJ
cord-333636-h2sg6shp	77	45	vortexing	vortexing	NN
cord-333636-h2sg6shp	77	46	.	.	.
cord-333636-h2sg6shp	78	1	After	after	IN
cord-333636-h2sg6shp	78	2	cell	cell	NN
cord-333636-h2sg6shp	78	3	lysis	lysis	NN
cord-333636-h2sg6shp	78	4	,	,	,
cord-333636-h2sg6shp	78	5	cellular	cellular	JJ
cord-333636-h2sg6shp	78	6	extracts	extract	NNS
cord-333636-h2sg6shp	78	7	were	be	VBD
cord-333636-h2sg6shp	78	8	mixed	mixed	JJ
cord-333636-h2sg6shp	78	9	(	(	-LRB-
cord-333636-h2sg6shp	78	10	1:1	1:1	LS
cord-333636-h2sg6shp	78	11	)	)	-RRB-
cord-333636-h2sg6shp	78	12	with	with	IN
cord-333636-h2sg6shp	78	13	loading	loading	NN
cord-333636-h2sg6shp	78	14	buffer	buffer	NN
cord-333636-h2sg6shp	78	15	(	(	-LRB-
cord-333636-h2sg6shp	78	16	16	16	CD
cord-333636-h2sg6shp	78	17	%	%	NN
cord-333636-h2sg6shp	78	18	glycerol	glycerol	NN
cord-333636-h2sg6shp	78	19	,	,	,
cord-333636-h2sg6shp	78	20	0.2	0.2	CD
cord-333636-h2sg6shp	78	21	%	%	NN
cord-333636-h2sg6shp	78	22	SDS	SDS	NNP
cord-333636-h2sg6shp	78	23	,	,	,
cord-333636-h2sg6shp	78	24	2	2	CD
cord-333636-h2sg6shp	78	25	mM	mm	NN
cord-333636-h2sg6shp	78	26	DTT	DTT	NNP
cord-333636-h2sg6shp	78	27	,	,	,
cord-333636-h2sg6shp	78	28	0.2	0.2	CD
cord-333636-h2sg6shp	78	29	%	%	NN
cord-333636-h2sg6shp	78	30	bromophenol	bromophenol	NN
cord-333636-h2sg6shp	78	31	blue	blue	JJ
cord-333636-h2sg6shp	78	32	,	,	,
cord-333636-h2sg6shp	78	33	24	24	CD
cord-333636-h2sg6shp	78	34	mM	mM	NNP
cord-333636-h2sg6shp	78	35	Tris	Tris	NNP
cord-333636-h2sg6shp	78	36	,	,	,
cord-333636-h2sg6shp	78	37	pH	pH	NNP
cord-333636-h2sg6shp	78	38	6.8	6.8	CD
cord-333636-h2sg6shp	78	39	in	in	IN
cord-333636-h2sg6shp	78	40	PBS	PBS	NNP
cord-333636-h2sg6shp	78	41	)	)	-RRB-
cord-333636-h2sg6shp	78	42	.	.	.
cord-333636-h2sg6shp	79	1	Samples	sample	NNS
cord-333636-h2sg6shp	79	2	were	be	VBD
cord-333636-h2sg6shp	79	3	incubated	incubate	VBN
cord-333636-h2sg6shp	79	4	at	at	IN
cord-333636-h2sg6shp	79	5	37	37	CD
cord-333636-h2sg6shp	79	6	•	•	NN
cord-333636-h2sg6shp	79	7	C	C	NNP
cord-333636-h2sg6shp	79	8	for	for	IN
cord-333636-h2sg6shp	79	9	10	10	CD
cord-333636-h2sg6shp	79	10	min	min	NN
cord-333636-h2sg6shp	79	11	with	with	IN
cord-333636-h2sg6shp	79	12	gentle	gentle	JJ
cord-333636-h2sg6shp	79	13	agitation	agitation	NN
cord-333636-h2sg6shp	79	14	and	and	CC
cord-333636-h2sg6shp	79	15	centrifuged	centrifuge	VBN
cord-333636-h2sg6shp	79	16	at	at	IN
cord-333636-h2sg6shp	79	17	1000	1000	CD
cord-333636-h2sg6shp	79	18	X	x	NN
cord-333636-h2sg6shp	79	19	g	g	NN
cord-333636-h2sg6shp	79	20	for	for	IN
cord-333636-h2sg6shp	79	21	5	5	CD
cord-333636-h2sg6shp	79	22	min	min	NN
cord-333636-h2sg6shp	79	23	at	at	IN
cord-333636-h2sg6shp	79	24	RT	RT	NNP
cord-333636-h2sg6shp	79	25	to	to	TO
cord-333636-h2sg6shp	79	26	remove	remove	VB
cord-333636-h2sg6shp	79	27	cell	cell	NN
cord-333636-h2sg6shp	79	28	debris	debris	NN
cord-333636-h2sg6shp	79	29	.	.	.
cord-333636-h2sg6shp	80	1	In	in	IN
cord-333636-h2sg6shp	80	2	all	all	DT
cord-333636-h2sg6shp	80	3	cases	case	NNS
cord-333636-h2sg6shp	80	4	100	100	CD
cord-333636-h2sg6shp	80	5	µg	µg	NNS
cord-333636-h2sg6shp	80	6	of	of	IN
cord-333636-h2sg6shp	80	7	cellular	cellular	JJ
cord-333636-h2sg6shp	80	8	extracts	extract	NNS
cord-333636-h2sg6shp	80	9	from	from	IN
cord-333636-h2sg6shp	80	10	cells	cell	NNS
cord-333636-h2sg6shp	80	11	infected	infect	VBN
cord-333636-h2sg6shp	80	12	either	either	CC
cord-333636-h2sg6shp	80	13	with	with	IN
cord-333636-h2sg6shp	80	14	WT	WT	NNP
cord-333636-h2sg6shp	80	15	BV	BV	NNP
cord-333636-h2sg6shp	80	16	or	or	CC
cord-333636-h2sg6shp	80	17	rBV	rBV	NNP
cord-333636-h2sg6shp	80	18	expressing	express	VBG
cord-333636-h2sg6shp	80	19	VP3	VP3	NNP
cord-333636-h2sg6shp	80	20	(	(	-LRB-
cord-333636-h2sg6shp	80	21	VP3-Bac	vp3-bac	NN
cord-333636-h2sg6shp	80	22	)	)	-RRB-
cord-333636-h2sg6shp	80	23	was	be	VBD
cord-333636-h2sg6shp	80	24	separated	separate	VBN
cord-333636-h2sg6shp	80	25	in	in	IN
cord-333636-h2sg6shp	80	26	12	12	CD
cord-333636-h2sg6shp	80	27	%	%	NN
cord-333636-h2sg6shp	80	28	SDS	sds	NN
cord-333636-h2sg6shp	80	29	-	-	HYPH
cord-333636-h2sg6shp	80	30	PAGE	page	NN
cord-333636-h2sg6shp	80	31	gels	gel	NNS
cord-333636-h2sg6shp	80	32	and	and	CC
cord-333636-h2sg6shp	80	33	transferred	transfer	VBN
cord-333636-h2sg6shp	80	34	onto	onto	IN
cord-333636-h2sg6shp	80	35	nitrocellulose	nitrocellulose	JJ
cord-333636-h2sg6shp	80	36	filters	filter	NNS
cord-333636-h2sg6shp	80	37	in	in	IN
cord-333636-h2sg6shp	80	38	Tris	Tris	NNP
cord-333636-h2sg6shp	80	39	-	-	HYPH
cord-333636-h2sg6shp	80	40	glycine	glycine	NN
cord-333636-h2sg6shp	80	41	buffer	buffer	NN
cord-333636-h2sg6shp	80	42	(	(	-LRB-
cord-333636-h2sg6shp	80	43	25	25	CD
cord-333636-h2sg6shp	80	44	mM	mm	CD
cord-333636-h2sg6shp	80	45	Tris	Tris	NNP
cord-333636-h2sg6shp	80	46	,	,	,
cord-333636-h2sg6shp	80	47	192	192	CD
cord-333636-h2sg6shp	80	48	mM	mm	CD
cord-333636-h2sg6shp	80	49	glycine	glycine	NN
cord-333636-h2sg6shp	80	50	,	,	,
cord-333636-h2sg6shp	80	51	pH	pH	NNP
cord-333636-h2sg6shp	80	52	8.3	8.3	CD
cord-333636-h2sg6shp	80	53	)	)	-RRB-
cord-333636-h2sg6shp	80	54	.	.	.
cord-333636-h2sg6shp	81	1	Filters	filter	NNS
cord-333636-h2sg6shp	81	2	were	be	VBD
cord-333636-h2sg6shp	81	3	incubated	incubate	VBN
cord-333636-h2sg6shp	81	4	16	16	CD
cord-333636-h2sg6shp	81	5	h	h	NN
cord-333636-h2sg6shp	81	6	at	at	IN
cord-333636-h2sg6shp	81	7	4	4	CD
cord-333636-h2sg6shp	82	1	•	•	NNP
cord-333636-h2sg6shp	83	1	C	C	NNP
cord-333636-h2sg6shp	83	2	in	in	IN
cord-333636-h2sg6shp	83	3	renaturing	renature	VBG
cord-333636-h2sg6shp	83	4	buffer	buffer	NN
cord-333636-h2sg6shp	83	5	(	(	-LRB-
cord-333636-h2sg6shp	83	6	50	50	CD
cord-333636-h2sg6shp	83	7	mM	mm	CD
cord-333636-h2sg6shp	83	8	NaCl	nacl	NN
cord-333636-h2sg6shp	83	9	,	,	,
cord-333636-h2sg6shp	83	10	1	1	CD
cord-333636-h2sg6shp	83	11	mM	mm	CD
cord-333636-h2sg6shp	83	12	EDTA	EDTA	NNP
cord-333636-h2sg6shp	83	13	,	,	,
cord-333636-h2sg6shp	83	14	0.02	0.02	CD
cord-333636-h2sg6shp	83	15	%	%	NN
cord-333636-h2sg6shp	83	16	Ficoll	Ficoll	NNP
cord-333636-h2sg6shp	83	17	,	,	,
cord-333636-h2sg6shp	83	18	0.02	0.02	CD
cord-333636-h2sg6shp	83	19	%	%	NN
cord-333636-h2sg6shp	83	20	bovine	bovine	JJ
cord-333636-h2sg6shp	83	21	serum	serum	NN
cord-333636-h2sg6shp	83	22	albumin	albumin	NN
cord-333636-h2sg6shp	83	23	(	(	-LRB-
cord-333636-h2sg6shp	83	24	BSA	BSA	NNP
cord-333636-h2sg6shp	83	25	)	)	-RRB-
cord-333636-h2sg6shp	83	26	,	,	,
cord-333636-h2sg6shp	83	27	0.02	0.02	CD
cord-333636-h2sg6shp	83	28	%	%	NN
cord-333636-h2sg6shp	83	29	polyvinylopirolidone	polyvinylopirolidone	NN
cord-333636-h2sg6shp	83	30	,	,	,
cord-333636-h2sg6shp	83	31	0.1	0.1	CD
cord-333636-h2sg6shp	83	32	%	%	NN
cord-333636-h2sg6shp	84	1	Triton	Triton	NNP
cord-333636-h2sg6shp	84	2	X-100	x-100	NN
cord-333636-h2sg6shp	84	3	,	,	,
cord-333636-h2sg6shp	84	4	10	10	CD
cord-333636-h2sg6shp	84	5	mM	mm	CD
cord-333636-h2sg6shp	84	6	Tris	Tris	NNP
cord-333636-h2sg6shp	84	7	,	,	,
cord-333636-h2sg6shp	84	8	pH	pH	NNP
cord-333636-h2sg6shp	84	9	7.5	7.5	CD
cord-333636-h2sg6shp	84	10	)	)	-RRB-
cord-333636-h2sg6shp	84	11	.	.	.
cord-333636-h2sg6shp	85	1	Afterwards	afterwards	RB
cord-333636-h2sg6shp	85	2	,	,	,
cord-333636-h2sg6shp	85	3	membranes	membrane	NNS
cord-333636-h2sg6shp	85	4	were	be	VBD
cord-333636-h2sg6shp	85	5	incubated	incubate	VBN
cord-333636-h2sg6shp	85	6	in	in	IN
cord-333636-h2sg6shp	85	7	10	10	CD
cord-333636-h2sg6shp	85	8	ml	ml	NNS
cord-333636-h2sg6shp	85	9	of	of	IN
cord-333636-h2sg6shp	85	10	renaturing	renature	VBG
cord-333636-h2sg6shp	85	11	buffer	buffer	NN
cord-333636-h2sg6shp	85	12	containing	contain	VBG
cord-333636-h2sg6shp	85	13	10	10	CD
cord-333636-h2sg6shp	85	14	6	6	CD
cord-333636-h2sg6shp	85	15	cpm	cpm	NNS
cord-333636-h2sg6shp	85	16	of	of	IN
cord-333636-h2sg6shp	85	17	32	32	CD
cord-333636-h2sg6shp	85	18	P	p	NN
cord-333636-h2sg6shp	85	19	-	-	HYPH
cord-333636-h2sg6shp	85	20	labeled	label	VBN
cord-333636-h2sg6shp	85	21	probe	probe	NN
cord-333636-h2sg6shp	85	22	B	b	NN
cord-333636-h2sg6shp	85	23	(	(	-LRB-
cord-333636-h2sg6shp	85	24	0.5	0.5	CD
cord-333636-h2sg6shp	85	25	ng	ng	NN
cord-333636-h2sg6shp	85	26	/	/	SYM
cord-333636-h2sg6shp	85	27	ml	ml	NNP
cord-333636-h2sg6shp	85	28	)	)	-RRB-
cord-333636-h2sg6shp	85	29	in	in	IN
cord-333636-h2sg6shp	85	30	the	the	DT
cord-333636-h2sg6shp	85	31	presence	presence	NN
cord-333636-h2sg6shp	85	32	of	of	IN
cord-333636-h2sg6shp	85	33	an	an	DT
cord-333636-h2sg6shp	85	34	excess	excess	JJ
cord-333636-h2sg6shp	85	35	(	(	-LRB-
cord-333636-h2sg6shp	85	36	10	10	CD
cord-333636-h2sg6shp	85	37	4	4	CD
cord-333636-h2sg6shp	85	38	ng	ng	NN
cord-333636-h2sg6shp	85	39	/	/	SYM
cord-333636-h2sg6shp	85	40	ml	ml	NN
cord-333636-h2sg6shp	85	41	)	)	-RRB-
cord-333636-h2sg6shp	85	42	of	of	IN
cord-333636-h2sg6shp	85	43	yeast	yeast	NN
cord-333636-h2sg6shp	85	44	whole	whole	JJ
cord-333636-h2sg6shp	85	45	RNA	RNA	NNP
cord-333636-h2sg6shp	85	46	over	over	IN
cord-333636-h2sg6shp	85	47	labeled	label	VBN
cord-333636-h2sg6shp	85	48	probe	probe	NN
cord-333636-h2sg6shp	85	49	.	.	.
cord-333636-h2sg6shp	86	1	After	after	IN
cord-333636-h2sg6shp	86	2	incubation	incubation	NN
cord-333636-h2sg6shp	86	3	for	for	IN
cord-333636-h2sg6shp	86	4	2	2	CD
cord-333636-h2sg6shp	86	5	h	h	NN
cord-333636-h2sg6shp	86	6	,	,	,
cord-333636-h2sg6shp	86	7	membranes	membrane	NNS
cord-333636-h2sg6shp	86	8	were	be	VBD
cord-333636-h2sg6shp	86	9	washed	wash	VBN
cord-333636-h2sg6shp	86	10	four	four	CD
cord-333636-h2sg6shp	86	11	times	time	NNS
cord-333636-h2sg6shp	86	12	with	with	IN
cord-333636-h2sg6shp	86	13	renaturing	renature	VBG
cord-333636-h2sg6shp	86	14	buffer	buffer	NN
cord-333636-h2sg6shp	86	15	for	for	IN
cord-333636-h2sg6shp	86	16	an	an	DT
cord-333636-h2sg6shp	86	17	additional	additional	JJ
cord-333636-h2sg6shp	86	18	hour	hour	NN
cord-333636-h2sg6shp	86	19	to	to	TO
cord-333636-h2sg6shp	86	20	remove	remove	VB
cord-333636-h2sg6shp	86	21	unbound	unbound	JJ
cord-333636-h2sg6shp	86	22	probe	probe	NN
cord-333636-h2sg6shp	86	23	.	.	.
cord-333636-h2sg6shp	87	1	After	after	IN
cord-333636-h2sg6shp	87	2	drying	dry	VBG
cord-333636-h2sg6shp	87	3	,	,	,
cord-333636-h2sg6shp	87	4	membranes	membrane	NNS
cord-333636-h2sg6shp	87	5	were	be	VBD
cord-333636-h2sg6shp	87	6	analyzed	analyze	VBN
cord-333636-h2sg6shp	87	7	with	with	IN
cord-333636-h2sg6shp	87	8	a	a	DT
cord-333636-h2sg6shp	87	9	phosphorimager	phosphorimager	NN
cord-333636-h2sg6shp	87	10	(	(	-LRB-
cord-333636-h2sg6shp	87	11	STORM	storm	NN
cord-333636-h2sg6shp	87	12	860	860	CD
cord-333636-h2sg6shp	87	13	,	,	,
cord-333636-h2sg6shp	87	14	Molecular	Molecular	NNP
cord-333636-h2sg6shp	87	15	Dynamics	Dynamics	NNPS
cord-333636-h2sg6shp	87	16	)	)	-RRB-
cord-333636-h2sg6shp	87	17	.	.	.
cord-333636-h2sg6shp	88	1	The	the	DT
cord-333636-h2sg6shp	88	2	same	same	JJ
cord-333636-h2sg6shp	88	3	membranes	membrane	NNS
cord-333636-h2sg6shp	88	4	were	be	VBD
cord-333636-h2sg6shp	88	5	processed	process	VBN
cord-333636-h2sg6shp	88	6	for	for	IN
cord-333636-h2sg6shp	88	7	Western	western	JJ
cord-333636-h2sg6shp	88	8	blot	blot	NN
cord-333636-h2sg6shp	88	9	using	use	VBG
cord-333636-h2sg6shp	88	10	specific	specific	JJ
cord-333636-h2sg6shp	88	11	anti	anti	JJ
cord-333636-h2sg6shp	88	12	-	-	JJ
cord-333636-h2sg6shp	88	13	VP3	vp3	JJ
cord-333636-h2sg6shp	88	14	antibodies	antibody	NNS
cord-333636-h2sg6shp	88	15	.	.	.
cord-333636-h2sg6shp	89	1	Experiments	experiment	NNS
cord-333636-h2sg6shp	89	2	were	be	VBD
cord-333636-h2sg6shp	89	3	repeated	repeat	VBN
cord-333636-h2sg6shp	89	4	three	three	CD
cord-333636-h2sg6shp	89	5	times	time	NNS
cord-333636-h2sg6shp	89	6	with	with	IN
cord-333636-h2sg6shp	89	7	independent	independent	JJ
cord-333636-h2sg6shp	89	8	preparations	preparation	NNS
cord-333636-h2sg6shp	89	9	of	of	IN
cord-333636-h2sg6shp	89	10	cell	cell	NN
cord-333636-h2sg6shp	89	11	extracts	extract	NNS
cord-333636-h2sg6shp	89	12	.	.	.
cord-333636-h2sg6shp	90	1	To	to	TO
cord-333636-h2sg6shp	90	2	characterize	characterize	VB
cord-333636-h2sg6shp	90	3	the	the	DT
cord-333636-h2sg6shp	90	4	VP3	VP3	NNP
cord-333636-h2sg6shp	90	5	ssRNA	ssrna	RB
cord-333636-h2sg6shp	90	6	-	-	HYPH
cord-333636-h2sg6shp	90	7	binding	bind	VBG
cord-333636-h2sg6shp	90	8	activity	activity	NN
cord-333636-h2sg6shp	90	9	,	,	,
cord-333636-h2sg6shp	90	10	a	a	DT
cord-333636-h2sg6shp	90	11	filter	filter	NN
cord-333636-h2sg6shp	90	12	-	-	HYPH
cord-333636-h2sg6shp	90	13	binding	bind	VBG
cord-333636-h2sg6shp	90	14	assay	assay	NN
cord-333636-h2sg6shp	90	15	was	be	VBD
cord-333636-h2sg6shp	90	16	performed	perform	VBN
cord-333636-h2sg6shp	90	17	as	as	IN
cord-333636-h2sg6shp	90	18	described	describe	VBN
cord-333636-h2sg6shp	90	19	[	[	-LRB-
cord-333636-h2sg6shp	90	20	21	21	CD
cord-333636-h2sg6shp	90	21	]	]	-RRB-
cord-333636-h2sg6shp	90	22	.	.	.
cord-333636-h2sg6shp	91	1	The	the	DT
cord-333636-h2sg6shp	91	2	affinity	affinity	NN
cord-333636-h2sg6shp	91	3	of	of	IN
cord-333636-h2sg6shp	91	4	VP3	VP3	NNP
cord-333636-h2sg6shp	91	5	protein	protein	NN
cord-333636-h2sg6shp	91	6	to	to	IN
cord-333636-h2sg6shp	91	7	ssRNA	ssRNA	VBG
cord-333636-h2sg6shp	91	8	was	be	VBD
cord-333636-h2sg6shp	91	9	estimated	estimate	VBN
cord-333636-h2sg6shp	91	10	from	from	IN
cord-333636-h2sg6shp	91	11	three	three	CD
cord-333636-h2sg6shp	91	12	independent	independent	JJ
cord-333636-h2sg6shp	91	13	experiments	experiment	NNS
cord-333636-h2sg6shp	91	14	using	use	VBG
cord-333636-h2sg6shp	91	15	32	32	CD
cord-333636-h2sg6shp	91	16	P	p	NN
cord-333636-h2sg6shp	91	17	-	-	HYPH
cord-333636-h2sg6shp	91	18	labeled	label	VBN
cord-333636-h2sg6shp	91	19	virus	virus	NN
cord-333636-h2sg6shp	91	20	ssRNA	ssrna	NN
cord-333636-h2sg6shp	91	21	probes	probe	NNS
cord-333636-h2sg6shp	91	22	.	.	.
cord-333636-h2sg6shp	92	1	Briefly	briefly	RB
cord-333636-h2sg6shp	92	2	,	,	,
cord-333636-h2sg6shp	92	3	increasing	increase	VBG
cord-333636-h2sg6shp	92	4	amounts	amount	NNS
cord-333636-h2sg6shp	92	5	(	(	-LRB-
cord-333636-h2sg6shp	92	6	from	from	IN
cord-333636-h2sg6shp	92	7	5	5	CD
cord-333636-h2sg6shp	92	8	×	×	CD
cord-333636-h2sg6shp	92	9	10	10	CD
cord-333636-h2sg6shp	92	10	−2	−2	NNP
cord-333636-h2sg6shp	92	11	mM	mm	CD
cord-333636-h2sg6shp	92	12	to	to	IN
cord-333636-h2sg6shp	92	13	5	5	CD
cord-333636-h2sg6shp	92	14	×	×	CD
cord-333636-h2sg6shp	92	15	10	10	CD
cord-333636-h2sg6shp	92	16	−6	−6	NNP
cord-333636-h2sg6shp	92	17	mM	mm	CD
cord-333636-h2sg6shp	92	18	)	)	-RRB-
cord-333636-h2sg6shp	92	19	of	of	IN
cord-333636-h2sg6shp	92	20	purified	purify	VBN
cord-333636-h2sg6shp	92	21	VP3	VP3	NNP
cord-333636-h2sg6shp	92	22	or	or	CC
cord-333636-h2sg6shp	92	23	BSA	BSA	NNP
cord-333636-h2sg6shp	92	24	,	,	,
cord-333636-h2sg6shp	92	25	used	use	VBN
cord-333636-h2sg6shp	92	26	as	as	IN
cord-333636-h2sg6shp	92	27	a	a	DT
cord-333636-h2sg6shp	92	28	control	control	NN
cord-333636-h2sg6shp	92	29	for	for	IN
cord-333636-h2sg6shp	92	30	non	non	JJ
cord-333636-h2sg6shp	92	31	-	-	JJ
cord-333636-h2sg6shp	92	32	specific	specific	JJ
cord-333636-h2sg6shp	92	33	interaction	interaction	NN
cord-333636-h2sg6shp	92	34	,	,	,
cord-333636-h2sg6shp	92	35	were	be	VBD
cord-333636-h2sg6shp	92	36	mixed	mix	VBN
cord-333636-h2sg6shp	92	37	with	with	IN
cord-333636-h2sg6shp	92	38	10	10	CD
cord-333636-h2sg6shp	92	39	6	6	CD
cord-333636-h2sg6shp	92	40	cpm	cpm	NNS
cord-333636-h2sg6shp	92	41	of	of	IN
cord-333636-h2sg6shp	92	42	32	32	CD
cord-333636-h2sg6shp	92	43	P	p	NN
cord-333636-h2sg6shp	92	44	-	-	HYPH
cord-333636-h2sg6shp	92	45	labeled	label	VBN
cord-333636-h2sg6shp	92	46	ssRNA	ssrna	NN
cord-333636-h2sg6shp	92	47	probe	probe	NN
cord-333636-h2sg6shp	92	48	B	B	NNP
cord-333636-h2sg6shp	92	49	(	(	-LRB-
cord-333636-h2sg6shp	92	50	5	5	CD
cord-333636-h2sg6shp	92	51	×	×	NNP
cord-333636-h2sg6shp	92	52	10	10	CD
cord-333636-h2sg6shp	92	53	−6	−6	NNP
cord-333636-h2sg6shp	92	54	mM	mm	CD
cord-333636-h2sg6shp	92	55	)	)	-RRB-
cord-333636-h2sg6shp	92	56	in	in	IN
cord-333636-h2sg6shp	92	57	binding	bind	VBG
cord-333636-h2sg6shp	92	58	reaction	reaction	NN
cord-333636-h2sg6shp	92	59	buffer	buffer	NN
cord-333636-h2sg6shp	92	60	(	(	-LRB-
cord-333636-h2sg6shp	92	61	50	50	CD
cord-333636-h2sg6shp	92	62	mM	mm	CD
cord-333636-h2sg6shp	92	63	Tris	Tris	NNP
cord-333636-h2sg6shp	92	64	,	,	,
cord-333636-h2sg6shp	92	65	150	150	CD
cord-333636-h2sg6shp	92	66	mM	mm	CD
cord-333636-h2sg6shp	92	67	KCl	KCl	NNS
cord-333636-h2sg6shp	92	68	,	,	,
cord-333636-h2sg6shp	92	69	150	150	CD
cord-333636-h2sg6shp	92	70	mM	mm	CD
cord-333636-h2sg6shp	92	71	NaCl	nacl	NN
cord-333636-h2sg6shp	92	72	10	10	CD
cord-333636-h2sg6shp	92	73	%	%	NN
cord-333636-h2sg6shp	92	74	Glycerol	glycerol	NN
cord-333636-h2sg6shp	92	75	,	,	,
cord-333636-h2sg6shp	92	76	0,01	0,01	CD
cord-333636-h2sg6shp	92	77	%	%	NN
cord-333636-h2sg6shp	92	78	Triton	Triton	NNP
cord-333636-h2sg6shp	92	79	X	X	NNP
cord-333636-h2sg6shp	92	80	100	100	CD
cord-333636-h2sg6shp	92	81	)	)	-RRB-
cord-333636-h2sg6shp	92	82	,	,	,
cord-333636-h2sg6shp	92	83	in	in	IN
cord-333636-h2sg6shp	92	84	16	16	CD
cord-333636-h2sg6shp	92	85	µl	µl	IN
cord-333636-h2sg6shp	92	86	final	final	JJ
cord-333636-h2sg6shp	92	87	volume	volume	NN
cord-333636-h2sg6shp	92	88	,	,	,
cord-333636-h2sg6shp	92	89	so	so	IN
cord-333636-h2sg6shp	92	90	the	the	DT
cord-333636-h2sg6shp	92	91	VP3	VP3	NNP
cord-333636-h2sg6shp	92	92	/	/	SYM
cord-333636-h2sg6shp	92	93	RNA	RNA	NNP
cord-333636-h2sg6shp	92	94	molar	molar	JJ
cord-333636-h2sg6shp	92	95	ratio	ratio	NN
cord-333636-h2sg6shp	92	96	in	in	IN
cord-333636-h2sg6shp	92	97	these	these	DT
cord-333636-h2sg6shp	92	98	experiments	experiment	NNS
cord-333636-h2sg6shp	92	99	varied	vary	VBN
cord-333636-h2sg6shp	92	100	from	from	IN
cord-333636-h2sg6shp	92	101	10	10	CD
cord-333636-h2sg6shp	92	102	4	4	CD
cord-333636-h2sg6shp	92	103	to	to	IN
cord-333636-h2sg6shp	92	104	1	1	CD
cord-333636-h2sg6shp	92	105	.	.	.
cord-333636-h2sg6shp	93	1	Binding	bind	VBG
cord-333636-h2sg6shp	93	2	reaction	reaction	NN
cord-333636-h2sg6shp	93	3	mixtures	mixture	NNS
cord-333636-h2sg6shp	93	4	were	be	VBD
cord-333636-h2sg6shp	93	5	incubated	incubate	VBN
cord-333636-h2sg6shp	93	6	at	at	IN
cord-333636-h2sg6shp	93	7	RT	RT	NNP
cord-333636-h2sg6shp	93	8	for	for	IN
cord-333636-h2sg6shp	93	9	1	1	CD
cord-333636-h2sg6shp	93	10	h	h	NN
cord-333636-h2sg6shp	93	11	and	and	CC
cord-333636-h2sg6shp	93	12	filtered	filter	VBN
cord-333636-h2sg6shp	93	13	through	through	IN
cord-333636-h2sg6shp	93	14	nitrocellulose	nitrocellulose	JJ
cord-333636-h2sg6shp	93	15	membranes	membrane	NNS
cord-333636-h2sg6shp	93	16	using	use	VBG
cord-333636-h2sg6shp	93	17	a	a	DT
cord-333636-h2sg6shp	93	18	dot	dot	NN
cord-333636-h2sg6shp	93	19	-	-	HYPH
cord-333636-h2sg6shp	93	20	blot	blot	NN
cord-333636-h2sg6shp	93	21	apparatus	apparatus	NN
cord-333636-h2sg6shp	93	22	.	.	.
cord-333636-h2sg6shp	94	1	In	in	IN
cord-333636-h2sg6shp	94	2	order	order	NN
cord-333636-h2sg6shp	94	3	to	to	TO
cord-333636-h2sg6shp	94	4	always	always	RB
cord-333636-h2sg6shp	94	5	use	use	VB
cord-333636-h2sg6shp	94	6	the	the	DT
cord-333636-h2sg6shp	94	7	same	same	JJ
cord-333636-h2sg6shp	94	8	protein	protein	NN
cord-333636-h2sg6shp	94	9	/	/	SYM
cord-333636-h2sg6shp	94	10	RNA	RNA	NNP
cord-333636-h2sg6shp	94	11	ratio	ratio	NN
cord-333636-h2sg6shp	94	12	,	,	,
cord-333636-h2sg6shp	94	13	when	when	WRB
cord-333636-h2sg6shp	94	14	other	other	JJ
cord-333636-h2sg6shp	94	15	probes	probe	NNS
cord-333636-h2sg6shp	94	16	were	be	VBD
cord-333636-h2sg6shp	94	17	used	use	VBN
cord-333636-h2sg6shp	94	18	the	the	DT
cord-333636-h2sg6shp	94	19	amount	amount	NN
cord-333636-h2sg6shp	94	20	of	of	IN
cord-333636-h2sg6shp	94	21	the	the	DT
cord-333636-h2sg6shp	94	22	probes	probe	NNS
cord-333636-h2sg6shp	94	23	were	be	VBD
cord-333636-h2sg6shp	94	24	corrected	correct	VBN
cord-333636-h2sg6shp	94	25	according	accord	VBG
cord-333636-h2sg6shp	94	26	to	to	IN
cord-333636-h2sg6shp	94	27	their	-PRON-	PRP$
cord-333636-h2sg6shp	94	28	size	size	NN
cord-333636-h2sg6shp	94	29	.	.	.
cord-333636-h2sg6shp	95	1	The	the	DT
cord-333636-h2sg6shp	95	2	proportion	proportion	NN
cord-333636-h2sg6shp	95	3	of	of	IN
cord-333636-h2sg6shp	95	4	the	the	DT
cord-333636-h2sg6shp	95	5	retained	retain	VBN
cord-333636-h2sg6shp	95	6	probe	probe	NN
cord-333636-h2sg6shp	95	7	in	in	IN
cord-333636-h2sg6shp	95	8	the	the	DT
cord-333636-h2sg6shp	95	9	filters	filter	NNS
cord-333636-h2sg6shp	95	10	was	be	VBD
cord-333636-h2sg6shp	95	11	quantified	quantify	VBN
cord-333636-h2sg6shp	95	12	by	by	IN
cord-333636-h2sg6shp	95	13	densitometry	densitometry	NN
cord-333636-h2sg6shp	95	14	using	use	VBG
cord-333636-h2sg6shp	95	15	a	a	DT
cord-333636-h2sg6shp	95	16	phosphorimager	phosphorimager	NN
cord-333636-h2sg6shp	95	17	apparatus	apparatus	NN
cord-333636-h2sg6shp	95	18	.	.	.
cord-333636-h2sg6shp	96	1	A	a	DT
cord-333636-h2sg6shp	96	2	VP3-RNA	VP3-RNA	NNP
cord-333636-h2sg6shp	96	3	binding	binding	NN
cord-333636-h2sg6shp	96	4	curve	curve	NN
cord-333636-h2sg6shp	96	5	was	be	VBD
cord-333636-h2sg6shp	96	6	drawn	draw	VBN
cord-333636-h2sg6shp	96	7	by	by	IN
cord-333636-h2sg6shp	96	8	plotting	plot	VBG
cord-333636-h2sg6shp	96	9	the	the	DT
cord-333636-h2sg6shp	96	10	percentage	percentage	NN
cord-333636-h2sg6shp	96	11	of	of	IN
cord-333636-h2sg6shp	96	12	retained	retain	VBN
cord-333636-h2sg6shp	96	13	radioactivity	radioactivity	NN
cord-333636-h2sg6shp	96	14	as	as	IN
cord-333636-h2sg6shp	96	15	a	a	DT
cord-333636-h2sg6shp	96	16	function	function	NN
cord-333636-h2sg6shp	96	17	of	of	IN
cord-333636-h2sg6shp	96	18	VP3	VP3	NNP
cord-333636-h2sg6shp	96	19	protein	protein	NN
cord-333636-h2sg6shp	96	20	concentration	concentration	NN
cord-333636-h2sg6shp	96	21	.	.	.
cord-333636-h2sg6shp	97	1	Assuming	assume	VBG
cord-333636-h2sg6shp	97	2	that	that	IN
cord-333636-h2sg6shp	97	3	the	the	DT
cord-333636-h2sg6shp	97	4	plateau	plateau	NN
cord-333636-h2sg6shp	97	5	reached	reach	VBD
cord-333636-h2sg6shp	97	6	represented	represent	VBD
cord-333636-h2sg6shp	97	7	the	the	DT
cord-333636-h2sg6shp	97	8	complete	complete	JJ
cord-333636-h2sg6shp	97	9	binding	binding	NN
cord-333636-h2sg6shp	97	10	of	of	IN
cord-333636-h2sg6shp	97	11	the	the	DT
cord-333636-h2sg6shp	97	12	RNA	RNA	NNP
cord-333636-h2sg6shp	97	13	,	,	,
cord-333636-h2sg6shp	97	14	the	the	DT
cord-333636-h2sg6shp	97	15	apparent	apparent	JJ
cord-333636-h2sg6shp	98	1	Kd	Kd	NNP
cord-333636-h2sg6shp	98	2	corresponded	correspond	VBD
cord-333636-h2sg6shp	98	3	to	to	IN
cord-333636-h2sg6shp	98	4	the	the	DT
cord-333636-h2sg6shp	98	5	concentration	concentration	NN
cord-333636-h2sg6shp	98	6	of	of	IN
cord-333636-h2sg6shp	98	7	VP3	VP3	NNP
cord-333636-h2sg6shp	98	8	protein	protein	NN
cord-333636-h2sg6shp	98	9	required	require	VBN
cord-333636-h2sg6shp	98	10	to	to	TO
cord-333636-h2sg6shp	98	11	reach	reach	VB
cord-333636-h2sg6shp	98	12	half	half	JJ
cord-333636-h2sg6shp	98	13	saturation	saturation	NN
cord-333636-h2sg6shp	98	14	[	[	-LRB-
cord-333636-h2sg6shp	98	15	21	21	CD
cord-333636-h2sg6shp	98	16	]	]	-RRB-
cord-333636-h2sg6shp	98	17	.	.	.
cord-333636-h2sg6shp	99	1	Kd	Kd	NNP
cord-333636-h2sg6shp	99	2	estimation	estimation	NN
cord-333636-h2sg6shp	99	3	was	be	VBD
cord-333636-h2sg6shp	99	4	performed	perform	VBN
cord-333636-h2sg6shp	99	5	assuming	assume	VBG
cord-333636-h2sg6shp	99	6	a	a	DT
cord-333636-h2sg6shp	99	7	simple	simple	JJ
cord-333636-h2sg6shp	99	8	molecule	molecule	NN
cord-333636-h2sg6shp	99	9	-	-	HYPH
cord-333636-h2sg6shp	99	10	to	to	IN
cord-333636-h2sg6shp	99	11	-	-	HYPH
cord-333636-h2sg6shp	99	12	molecule	molecule	NN
cord-333636-h2sg6shp	99	13	binding	bind	VBG
cord-333636-h2sg6shp	99	14	equilibrium	equilibrium	NN
cord-333636-h2sg6shp	99	15	reaction	reaction	NN
cord-333636-h2sg6shp	99	16	.	.	.
cord-333636-h2sg6shp	100	1	To	to	TO
cord-333636-h2sg6shp	100	2	transform	transform	VB
cord-333636-h2sg6shp	100	3	the	the	DT
cord-333636-h2sg6shp	100	4	non	non	JJ
cord-333636-h2sg6shp	100	5	-	-	JJ
cord-333636-h2sg6shp	100	6	linear	linear	JJ
cord-333636-h2sg6shp	100	7	curve	curve	NN
cord-333636-h2sg6shp	100	8	observed	observe	VBN
cord-333636-h2sg6shp	100	9	for	for	IN
cord-333636-h2sg6shp	100	10	VP3-RNA	VP3-RNA	NNP
cord-333636-h2sg6shp	100	11	binding	bind	VBG
cord-333636-h2sg6shp	100	12	to	to	IN
cord-333636-h2sg6shp	100	13	linear	linear	NNP
cord-333636-h2sg6shp	100	14	,	,	,
cord-333636-h2sg6shp	100	15	a	a	DT
cord-333636-h2sg6shp	100	16	Hill	Hill	NNP
cord-333636-h2sg6shp	100	17	plot	plot	NN
cord-333636-h2sg6shp	100	18	was	be	VBD
cord-333636-h2sg6shp	100	19	obtained	obtain	VBN
cord-333636-h2sg6shp	100	20	as	as	IN
cord-333636-h2sg6shp	100	21	described	describe	VBN
cord-333636-h2sg6shp	100	22	[	[	-LRB-
cord-333636-h2sg6shp	100	23	3	3	CD
cord-333636-h2sg6shp	100	24	,	,	,
cord-333636-h2sg6shp	100	25	5	5	CD
cord-333636-h2sg6shp	100	26	]	]	-RRB-
cord-333636-h2sg6shp	100	27	,	,	,
cord-333636-h2sg6shp	100	28	by	by	IN
cord-333636-h2sg6shp	100	29	plotting	plot	VBG
cord-333636-h2sg6shp	100	30	Log	Log	NNP
cord-333636-h2sg6shp	100	31	[	[	-LRB-
cord-333636-h2sg6shp	100	32	retained	retain	VBD
cord-333636-h2sg6shp	100	33	radioactivity/(100-retained	radioactivity/(100-retained	NNP
cord-333636-h2sg6shp	100	34	radioactivity	radioactivity	NN
cord-333636-h2sg6shp	100	35	)	)	-RRB-
cord-333636-h2sg6shp	100	36	]	]	-RRB-
cord-333636-h2sg6shp	100	37	as	as	IN
cord-333636-h2sg6shp	100	38	a	a	DT
cord-333636-h2sg6shp	100	39	function	function	NN
cord-333636-h2sg6shp	100	40	of	of	IN
cord-333636-h2sg6shp	100	41	Log	Log	NNP
cord-333636-h2sg6shp	100	42	(	(	-LRB-
cord-333636-h2sg6shp	100	43	VP3	VP3	NNP
cord-333636-h2sg6shp	100	44	concentration	concentration	NN
cord-333636-h2sg6shp	100	45	)	)	-RRB-
cord-333636-h2sg6shp	100	46	.	.	.
cord-333636-h2sg6shp	101	1	For	for	IN
cord-333636-h2sg6shp	101	2	the	the	DT
cord-333636-h2sg6shp	101	3	Hill	Hill	NNP
cord-333636-h2sg6shp	101	4	plot	plot	NN
cord-333636-h2sg6shp	101	5	analysis	analysis	NN
cord-333636-h2sg6shp	101	6	,	,	,
cord-333636-h2sg6shp	101	7	means	mean	NNS
cord-333636-h2sg6shp	101	8	of	of	IN
cord-333636-h2sg6shp	101	9	retained	retain	VBN
cord-333636-h2sg6shp	101	10	radioactivity	radioactivity	NN
cord-333636-h2sg6shp	101	11	from	from	IN
cord-333636-h2sg6shp	101	12	three	three	CD
cord-333636-h2sg6shp	101	13	independent	independent	JJ
cord-333636-h2sg6shp	101	14	experiments	experiment	NNS
cord-333636-h2sg6shp	101	15	were	be	VBD
cord-333636-h2sg6shp	101	16	used	use	VBN
cord-333636-h2sg6shp	101	17	.	.	.
cord-333636-h2sg6shp	102	1	A	a	DT
cord-333636-h2sg6shp	102	2	linear	linear	JJ
cord-333636-h2sg6shp	102	3	regression	regression	NN
cord-333636-h2sg6shp	102	4	was	be	VBD
cord-333636-h2sg6shp	102	5	performed	perform	VBN
cord-333636-h2sg6shp	102	6	by	by	IN
cord-333636-h2sg6shp	102	7	the	the	DT
cord-333636-h2sg6shp	102	8	least	least	JJS
cord-333636-h2sg6shp	102	9	squares	square	NNS
cord-333636-h2sg6shp	102	10	procedure	procedure	NN
cord-333636-h2sg6shp	102	11	(	(	-LRB-
cord-333636-h2sg6shp	102	12	9	9	CD
cord-333636-h2sg6shp	102	13	degrees	degree	NNS
cord-333636-h2sg6shp	102	14	of	of	IN
cord-333636-h2sg6shp	102	15	freedom	freedom	NN
cord-333636-h2sg6shp	102	16	)	)	-RRB-
cord-333636-h2sg6shp	102	17	and	and	CC
cord-333636-h2sg6shp	103	1	the	the	DT
cord-333636-h2sg6shp	103	2	associated	associated	JJ
cord-333636-h2sg6shp	103	3	probability	probability	NN
cord-333636-h2sg6shp	103	4	to	to	IN
cord-333636-h2sg6shp	103	5	the	the	DT
cord-333636-h2sg6shp	103	6	correlation	correlation	NN
cord-333636-h2sg6shp	103	7	coefficient	coefficient	NN
cord-333636-h2sg6shp	103	8	(	(	-LRB-
cord-333636-h2sg6shp	103	9	R	r	NN
cord-333636-h2sg6shp	103	10	)	)	-RRB-
cord-333636-h2sg6shp	103	11	was	be	VBD
cord-333636-h2sg6shp	103	12	calculated	calculate	VBN
cord-333636-h2sg6shp	103	13	obtaining	obtain	VBG
cord-333636-h2sg6shp	103	14	a	a	DT
cord-333636-h2sg6shp	103	15	t	t	NN
cord-333636-h2sg6shp	103	16	value	value	NN
cord-333636-h2sg6shp	103	17	for	for	IN
cord-333636-h2sg6shp	103	18	a	a	DT
cord-333636-h2sg6shp	103	19	t	t	NN
cord-333636-h2sg6shp	103	20	-	-	HYPH
cord-333636-h2sg6shp	103	21	Student	Student	NNP
cord-333636-h2sg6shp	103	22	test	test	NN
cord-333636-h2sg6shp	103	23	,	,	,
cord-333636-h2sg6shp	103	24	as	as	IN
cord-333636-h2sg6shp	103	25	described	describe	VBN
cord-333636-h2sg6shp	103	26	[	[	-LRB-
cord-333636-h2sg6shp	103	27	37	37	CD
cord-333636-h2sg6shp	103	28	]	]	-RRB-
cord-333636-h2sg6shp	103	29	.	.	.
cord-333636-h2sg6shp	104	1	The	the	DT
cord-333636-h2sg6shp	104	2	Hill	Hill	NNP
cord-333636-h2sg6shp	104	3	coefficient	coefficient	NN
cord-333636-h2sg6shp	104	4	(	(	-LRB-
cord-333636-h2sg6shp	104	5	n	n	NNP
cord-333636-h2sg6shp	104	6	H	H	NNP
cord-333636-h2sg6shp	104	7	)	)	-RRB-
cord-333636-h2sg6shp	104	8	was	be	VBD
cord-333636-h2sg6shp	104	9	obtained	obtain	VBN
cord-333636-h2sg6shp	104	10	as	as	IN
cord-333636-h2sg6shp	104	11	the	the	DT
cord-333636-h2sg6shp	104	12	slope	slope	NN
cord-333636-h2sg6shp	104	13	of	of	IN
cord-333636-h2sg6shp	104	14	the	the	DT
cord-333636-h2sg6shp	104	15	regression	regression	NN
cord-333636-h2sg6shp	104	16	line	line	NN
cord-333636-h2sg6shp	104	17	and	and	CC
cord-333636-h2sg6shp	104	18	the	the	DT
cord-333636-h2sg6shp	104	19	K	K	NNP
cord-333636-h2sg6shp	104	20	0.5	0.5	CD
cord-333636-h2sg6shp	104	21	value	value	NN
cord-333636-h2sg6shp	104	22	(	(	-LRB-
cord-333636-h2sg6shp	104	23	amount	amount	NN
cord-333636-h2sg6shp	104	24	of	of	IN
cord-333636-h2sg6shp	104	25	VP3	vp3	JJ
cord-333636-h2sg6shp	104	26	concentration	concentration	NN
cord-333636-h2sg6shp	104	27	to	to	TO
cord-333636-h2sg6shp	104	28	give	give	VB
cord-333636-h2sg6shp	104	29	half	half	JJ
cord-333636-h2sg6shp	104	30	saturation	saturation	NN
cord-333636-h2sg6shp	104	31	,	,	,
cord-333636-h2sg6shp	104	32	apparent	apparent	JJ
cord-333636-h2sg6shp	104	33	Kd	Kd	NNP
cord-333636-h2sg6shp	104	34	)	)	-RRB-
cord-333636-h2sg6shp	104	35	was	be	VBD
cord-333636-h2sg6shp	104	36	calculated	calculate	VBN
cord-333636-h2sg6shp	104	37	[	[	-LRB-
cord-333636-h2sg6shp	104	38	3	3	CD
cord-333636-h2sg6shp	104	39	]	]	-RRB-
cord-333636-h2sg6shp	104	40	.	.	.
cord-333636-h2sg6shp	105	1	Competition	competition	NN
cord-333636-h2sg6shp	105	2	assays	assay	NNS
cord-333636-h2sg6shp	105	3	based	base	VBN
cord-333636-h2sg6shp	105	4	on	on	IN
cord-333636-h2sg6shp	105	5	nitrocellulose	nitrocellulose	NN
cord-333636-h2sg6shp	105	6	binding	binding	NN
cord-333636-h2sg6shp	105	7	were	be	VBD
cord-333636-h2sg6shp	105	8	carried	carry	VBN
cord-333636-h2sg6shp	105	9	out	out	RP
cord-333636-h2sg6shp	105	10	using	use	VBG
cord-333636-h2sg6shp	105	11	virus	virus	NN
cord-333636-h2sg6shp	105	12	-	-	HYPH
cord-333636-h2sg6shp	105	13	derived	derive	VBN
cord-333636-h2sg6shp	105	14	radiolabeled	radiolabele	VBN
cord-333636-h2sg6shp	105	15	ssRNA	ssrna	NN
cord-333636-h2sg6shp	105	16	probes	probe	NNS
cord-333636-h2sg6shp	105	17	together	together	RB
cord-333636-h2sg6shp	105	18	with	with	IN
cord-333636-h2sg6shp	105	19	increasing	increase	VBG
cord-333636-h2sg6shp	105	20	concentrations	concentration	NNS
cord-333636-h2sg6shp	105	21	of	of	IN
cord-333636-h2sg6shp	105	22	non	non	JJ
cord-333636-h2sg6shp	105	23	-	-	JJ
cord-333636-h2sg6shp	105	24	radioactive	radioactive	JJ
cord-333636-h2sg6shp	105	25	competitor	competitor	NN
cord-333636-h2sg6shp	105	26	RNA	RNA	NNP
cord-333636-h2sg6shp	105	27	and	and	CC
cord-333636-h2sg6shp	105	28	DNA	dna	NN
cord-333636-h2sg6shp	105	29	probes	probe	NNS
cord-333636-h2sg6shp	105	30	.	.	.
cord-333636-h2sg6shp	106	1	Briefly	briefly	RB
cord-333636-h2sg6shp	106	2	,	,	,
cord-333636-h2sg6shp	106	3	VP3-RNA	VP3-RNA	NNP
cord-333636-h2sg6shp	106	4	binding	binding	NN
cord-333636-h2sg6shp	106	5	reactions	reaction	NNS
cord-333636-h2sg6shp	106	6	were	be	VBD
cord-333636-h2sg6shp	106	7	carried	carry	VBN
cord-333636-h2sg6shp	106	8	out	out	RP
cord-333636-h2sg6shp	106	9	as	as	IN
cord-333636-h2sg6shp	106	10	described	describe	VBN
cord-333636-h2sg6shp	106	11	above	above	IN
cord-333636-h2sg6shp	106	12	using	use	VBG
cord-333636-h2sg6shp	106	13	radiolabeled	radiolabele	VBN
cord-333636-h2sg6shp	106	14	ssRNA	ssRNA	''
cord-333636-h2sg6shp	107	1	probes	probe	NNS
cord-333636-h2sg6shp	107	2	B	B	NNP
cord-333636-h2sg6shp	107	3	and	and	CC
cord-333636-h2sg6shp	107	4	A.	a.	NN
cord-333636-h2sg6shp	107	5	Binding	Binding	NNP
cord-333636-h2sg6shp	107	6	reactions	reaction	NNS
cord-333636-h2sg6shp	107	7	were	be	VBD
cord-333636-h2sg6shp	107	8	performed	perform	VBN
cord-333636-h2sg6shp	107	9	in	in	IN
cord-333636-h2sg6shp	107	10	the	the	DT
cord-333636-h2sg6shp	107	11	presence	presence	NN
cord-333636-h2sg6shp	107	12	of	of	IN
cord-333636-h2sg6shp	107	13	increasing	increase	VBG
cord-333636-h2sg6shp	107	14	concentrations	concentration	NNS
cord-333636-h2sg6shp	107	15	,	,	,
cord-333636-h2sg6shp	107	16	molar	molar	JJ
cord-333636-h2sg6shp	107	17	ratios	ratio	NNS
cord-333636-h2sg6shp	107	18	of	of	IN
cord-333636-h2sg6shp	107	19	1:1	1:1	CD
cord-333636-h2sg6shp	107	20	,	,	,
cord-333636-h2sg6shp	107	21	1:10	1:10	CD
cord-333636-h2sg6shp	107	22	,	,	,
cord-333636-h2sg6shp	107	23	1:100	1:100	CD
cord-333636-h2sg6shp	107	24	and	and	CC
cord-333636-h2sg6shp	107	25	1:1000	1:1000	NNS
cord-333636-h2sg6shp	107	26	,	,	,
cord-333636-h2sg6shp	107	27	of	of	IN
cord-333636-h2sg6shp	107	28	competitor	competitor	NN
cord-333636-h2sg6shp	107	29	unlabeled	unlabeled	JJ
cord-333636-h2sg6shp	107	30	RNA	RNA	NNP
cord-333636-h2sg6shp	107	31	or	or	CC
cord-333636-h2sg6shp	107	32	DNA	dna	NN
cord-333636-h2sg6shp	107	33	probes	probe	NNS
cord-333636-h2sg6shp	107	34	.	.	.
cord-333636-h2sg6shp	108	1	Reaction	reaction	NN
cord-333636-h2sg6shp	108	2	mixtures	mixture	NNS
cord-333636-h2sg6shp	108	3	were	be	VBD
cord-333636-h2sg6shp	108	4	filtered	filter	VBN
cord-333636-h2sg6shp	108	5	through	through	IN
cord-333636-h2sg6shp	108	6	nitrocellulose	nitrocellulose	JJ
cord-333636-h2sg6shp	108	7	membranes	membrane	NNS
cord-333636-h2sg6shp	108	8	,	,	,
cord-333636-h2sg6shp	108	9	and	and	CC
cord-333636-h2sg6shp	108	10	the	the	DT
cord-333636-h2sg6shp	108	11	retained	retain	VBN
cord-333636-h2sg6shp	108	12	radioactivity	radioactivity	NN
cord-333636-h2sg6shp	108	13	was	be	VBD
cord-333636-h2sg6shp	108	14	quantified	quantify	VBN
cord-333636-h2sg6shp	108	15	as	as	IN
cord-333636-h2sg6shp	108	16	described	describe	VBN
cord-333636-h2sg6shp	108	17	above	above	RB
cord-333636-h2sg6shp	108	18	.	.	.
cord-333636-h2sg6shp	109	1	Competition	competition	NN
cord-333636-h2sg6shp	109	2	experiments	experiment	NNS
cord-333636-h2sg6shp	109	3	were	be	VBD
cord-333636-h2sg6shp	109	4	also	also	RB
cord-333636-h2sg6shp	109	5	repeated	repeat	VBN
cord-333636-h2sg6shp	109	6	three	three	CD
cord-333636-h2sg6shp	109	7	times	time	NNS
cord-333636-h2sg6shp	109	8	to	to	TO
cord-333636-h2sg6shp	109	9	obtain	obtain	VB
cord-333636-h2sg6shp	109	10	representative	representative	NN
cord-333636-h2sg6shp	109	11	means	mean	NNS
cord-333636-h2sg6shp	109	12	.	.	.
cord-333636-h2sg6shp	110	1	The	the	DT
cord-333636-h2sg6shp	110	2	interaction	interaction	NN
cord-333636-h2sg6shp	110	3	of	of	IN
cord-333636-h2sg6shp	110	4	VP3	VP3	NNP
cord-333636-h2sg6shp	110	5	with	with	IN
cord-333636-h2sg6shp	110	6	ssRNA	ssRNA	VBG
cord-333636-h2sg6shp	110	7	was	be	VBD
cord-333636-h2sg6shp	110	8	analyzed	analyze	VBN
cord-333636-h2sg6shp	110	9	by	by	IN
cord-333636-h2sg6shp	110	10	EMSA	EMSA	NNP
cord-333636-h2sg6shp	110	11	as	as	IN
cord-333636-h2sg6shp	110	12	described	describe	VBN
cord-333636-h2sg6shp	110	13	[	[	-LRB-
cord-333636-h2sg6shp	110	14	1	1	CD
cord-333636-h2sg6shp	110	15	,	,	,
cord-333636-h2sg6shp	110	16	5	5	CD
cord-333636-h2sg6shp	110	17	]	]	-RRB-
cord-333636-h2sg6shp	110	18	with	with	IN
cord-333636-h2sg6shp	110	19	minor	minor	JJ
cord-333636-h2sg6shp	110	20	modifications	modification	NNS
cord-333636-h2sg6shp	110	21	.	.	.
cord-333636-h2sg6shp	111	1	Briefly	briefly	RB
cord-333636-h2sg6shp	111	2	,	,	,
cord-333636-h2sg6shp	111	3	VP3-ssRNA	vp3-ssrna	NN
cord-333636-h2sg6shp	111	4	complexes	complex	NNS
cord-333636-h2sg6shp	111	5	were	be	VBD
cord-333636-h2sg6shp	111	6	allowed	allow	VBN
cord-333636-h2sg6shp	111	7	to	to	TO
cord-333636-h2sg6shp	111	8	form	form	VB
cord-333636-h2sg6shp	111	9	using	use	VBG
cord-333636-h2sg6shp	111	10	increasing	increase	VBG
cord-333636-h2sg6shp	111	11	amounts	amount	NNS
cord-333636-h2sg6shp	111	12	of	of	IN
cord-333636-h2sg6shp	111	13	purified	purify	VBN
cord-333636-h2sg6shp	111	14	VP3	VP3	NNP
cord-333636-h2sg6shp	111	15	as	as	IN
cord-333636-h2sg6shp	111	16	described	describe	VBN
cord-333636-h2sg6shp	111	17	above	above	RB
cord-333636-h2sg6shp	111	18	.	.	.
cord-333636-h2sg6shp	112	1	After	after	IN
cord-333636-h2sg6shp	112	2	incubation	incubation	NN
cord-333636-h2sg6shp	112	3	,	,	,
cord-333636-h2sg6shp	112	4	aliquots	aliquot	NNS
cord-333636-h2sg6shp	112	5	of	of	IN
cord-333636-h2sg6shp	112	6	the	the	DT
cord-333636-h2sg6shp	112	7	reaction	reaction	NN
cord-333636-h2sg6shp	112	8	mixtures	mixture	NNS
cord-333636-h2sg6shp	112	9	were	be	VBD
cord-333636-h2sg6shp	112	10	loaded	load	VBN
cord-333636-h2sg6shp	112	11	onto	onto	IN
cord-333636-h2sg6shp	112	12	7	7	CD
cord-333636-h2sg6shp	112	13	%	%	NN
cord-333636-h2sg6shp	112	14	polyacrylamide	polyacrylamide	NN
cord-333636-h2sg6shp	112	15	gel	gel	NN
cord-333636-h2sg6shp	112	16	in	in	IN
cord-333636-h2sg6shp	112	17	TBE	TBE	NNP
cord-333636-h2sg6shp	112	18	buffer	buffer	NN
cord-333636-h2sg6shp	112	19	(	(	-LRB-
cord-333636-h2sg6shp	112	20	45	45	CD
cord-333636-h2sg6shp	112	21	mM	mM	NNP
cord-333636-h2sg6shp	112	22	Tris	Tris	NNP
cord-333636-h2sg6shp	112	23	-	-	HYPH
cord-333636-h2sg6shp	112	24	borate	borate	NNP
cord-333636-h2sg6shp	112	25	,	,	,
cord-333636-h2sg6shp	112	26	1	1	CD
cord-333636-h2sg6shp	112	27	mM	mm	CD
cord-333636-h2sg6shp	112	28	EDTA	EDTA	NNP
cord-333636-h2sg6shp	112	29	,	,	,
cord-333636-h2sg6shp	112	30	pH	pH	NNP
cord-333636-h2sg6shp	112	31	8.0	8.0	CD
cord-333636-h2sg6shp	112	32	)	)	-RRB-
cord-333636-h2sg6shp	112	33	,	,	,
cord-333636-h2sg6shp	112	34	and	and	CC
cord-333636-h2sg6shp	112	35	gels	gel	NNS
cord-333636-h2sg6shp	112	36	were	be	VBD
cord-333636-h2sg6shp	112	37	run	run	VBN
cord-333636-h2sg6shp	112	38	in	in	IN
cord-333636-h2sg6shp	112	39	TBE	TBE	NNP
cord-333636-h2sg6shp	112	40	buffer	buffer	NN
cord-333636-h2sg6shp	112	41	at	at	IN
cord-333636-h2sg6shp	112	42	4	4	CD
cord-333636-h2sg6shp	112	43	•	•	NN
cord-333636-h2sg6shp	113	1	C.	C.	NNP
cord-333636-h2sg6shp	113	2	Gels	gel	NNS
cord-333636-h2sg6shp	113	3	were	be	VBD
cord-333636-h2sg6shp	113	4	dried	dry	VBN
cord-333636-h2sg6shp	113	5	and	and	CC
cord-333636-h2sg6shp	113	6	exposed	expose	VBN
cord-333636-h2sg6shp	113	7	using	use	VBG
cord-333636-h2sg6shp	113	8	a	a	DT
cord-333636-h2sg6shp	113	9	phophorimager	phophorimager	NN
cord-333636-h2sg6shp	113	10	apparatus	apparatus	NN
cord-333636-h2sg6shp	113	11	.	.	.
cord-333636-h2sg6shp	114	1	Excess	excess	NN
cord-333636-h2sg6shp	114	2	of	of	IN
cord-333636-h2sg6shp	114	3	BSA	BSA	NNP
cord-333636-h2sg6shp	114	4	(	(	-LRB-
cord-333636-h2sg6shp	114	5	from	from	IN
cord-333636-h2sg6shp	114	6	1	1	CD
cord-333636-h2sg6shp	114	7	to	to	IN
cord-333636-h2sg6shp	114	8	10	10	CD
cord-333636-h2sg6shp	114	9	mM	mm	CD
cord-333636-h2sg6shp	114	10	)	)	-RRB-
cord-333636-h2sg6shp	114	11	was	be	VBD
cord-333636-h2sg6shp	114	12	used	use	VBN
cord-333636-h2sg6shp	114	13	as	as	IN
cord-333636-h2sg6shp	114	14	a	a	DT
cord-333636-h2sg6shp	114	15	non	non	JJ
cord-333636-h2sg6shp	114	16	-	-	JJ
cord-333636-h2sg6shp	114	17	specific	specific	JJ
cord-333636-h2sg6shp	114	18	protein	protein	NN
cord-333636-h2sg6shp	114	19	in	in	IN
cord-333636-h2sg6shp	114	20	RNAbinding	rnabinding	NN
cord-333636-h2sg6shp	114	21	reactions	reaction	NNS
cord-333636-h2sg6shp	114	22	.	.	.
cord-333636-h2sg6shp	115	1	For	for	IN
cord-333636-h2sg6shp	115	2	supershift	supershift	NN
cord-333636-h2sg6shp	115	3	assays	assay	NNS
cord-333636-h2sg6shp	115	4	,	,	,
cord-333636-h2sg6shp	115	5	after	after	IN
cord-333636-h2sg6shp	115	6	a	a	DT
cord-333636-h2sg6shp	115	7	20	20	CD
cord-333636-h2sg6shp	115	8	min	min	NN
cord-333636-h2sg6shp	115	9	incubation	incubation	NN
cord-333636-h2sg6shp	115	10	at	at	IN
cord-333636-h2sg6shp	115	11	RT	RT	NNP
cord-333636-h2sg6shp	115	12	,	,	,
cord-333636-h2sg6shp	115	13	the	the	DT
cord-333636-h2sg6shp	115	14	reaction	reaction	NN
cord-333636-h2sg6shp	115	15	mixtures	mixture	NNS
cord-333636-h2sg6shp	115	16	were	be	VBD
cord-333636-h2sg6shp	115	17	supplemented	supplement	VBN
cord-333636-h2sg6shp	115	18	with	with	IN
cord-333636-h2sg6shp	115	19	1	1	CD
cord-333636-h2sg6shp	115	20	µg	µg	CD
cord-333636-h2sg6shp	115	21	of	of	IN
cord-333636-h2sg6shp	115	22	either	either	DT
cord-333636-h2sg6shp	115	23	VP3-specific	VP3-specific	NNP
cord-333636-h2sg6shp	115	24	monoclonal	monoclonal	JJ
cord-333636-h2sg6shp	115	25	antibodies	antibody	NNS
cord-333636-h2sg6shp	115	26	(	(	-LRB-
cord-333636-h2sg6shp	115	27	MAbs	MAbs	NNP
cord-333636-h2sg6shp	115	28	)	)	-RRB-
cord-333636-h2sg6shp	115	29	or	or	CC
cord-333636-h2sg6shp	115	30	nonspecific	nonspecific	JJ
cord-333636-h2sg6shp	115	31	antibodies	antibody	NNS
cord-333636-h2sg6shp	115	32	(	(	-LRB-
cord-333636-h2sg6shp	115	33	preimmune	preimmune	NNP
cord-333636-h2sg6shp	115	34	mouse	mouse	NNP
cord-333636-h2sg6shp	115	35	IgG	IgG	NNP
cord-333636-h2sg6shp	115	36	)	)	-RRB-
cord-333636-h2sg6shp	115	37	and	and	CC
cord-333636-h2sg6shp	115	38	maintained	maintain	VBD
cord-333636-h2sg6shp	115	39	for	for	IN
cord-333636-h2sg6shp	115	40	1	1	CD
cord-333636-h2sg6shp	115	41	h	h	NN
cord-333636-h2sg6shp	115	42	at	at	IN
cord-333636-h2sg6shp	115	43	RT	RT	NNP
cord-333636-h2sg6shp	115	44	.	.	.
cord-333636-h2sg6shp	116	1	Thereafter	thereafter	RB
cord-333636-h2sg6shp	116	2	,	,	,
cord-333636-h2sg6shp	116	3	reaction	reaction	NN
cord-333636-h2sg6shp	116	4	mixtures	mixture	NNS
cord-333636-h2sg6shp	116	5	were	be	VBD
cord-333636-h2sg6shp	116	6	resolved	resolve	VBN
cord-333636-h2sg6shp	116	7	in	in	IN
cord-333636-h2sg6shp	116	8	polyacrylamide	polyacrylamide	NN
cord-333636-h2sg6shp	116	9	gels	gel	NNS
cord-333636-h2sg6shp	116	10	and	and	CC
cord-333636-h2sg6shp	116	11	VP3-ssRNA	vp3-ssrna	NN
cord-333636-h2sg6shp	116	12	complexes	complex	NNS
cord-333636-h2sg6shp	116	13	were	be	VBD
cord-333636-h2sg6shp	116	14	revealed	reveal	VBN
cord-333636-h2sg6shp	116	15	as	as	IN
cord-333636-h2sg6shp	116	16	described	describe	VBN
cord-333636-h2sg6shp	116	17	above	above	RB
cord-333636-h2sg6shp	116	18	.	.	.
cord-333636-h2sg6shp	117	1	Experiments	experiment	NNS
cord-333636-h2sg6shp	117	2	were	be	VBD
cord-333636-h2sg6shp	117	3	repeated	repeat	VBN
cord-333636-h2sg6shp	117	4	three	three	CD
cord-333636-h2sg6shp	117	5	times	time	NNS
cord-333636-h2sg6shp	117	6	.	.	.
cord-333636-h2sg6shp	118	1	To	to	TO
cord-333636-h2sg6shp	118	2	find	find	VB
cord-333636-h2sg6shp	118	3	the	the	DT
cord-333636-h2sg6shp	118	4	level	level	NN
cord-333636-h2sg6shp	118	5	of	of	IN
cord-333636-h2sg6shp	118	6	sequence	sequence	NN
cord-333636-h2sg6shp	118	7	conservation	conservation	NN
cord-333636-h2sg6shp	118	8	of	of	IN
cord-333636-h2sg6shp	118	9	the	the	DT
cord-333636-h2sg6shp	118	10	VP3	VP3	NNP
cord-333636-h2sg6shp	118	11	ssRNA	ssrna	RB
cord-333636-h2sg6shp	118	12	-	-	HYPH
cord-333636-h2sg6shp	118	13	binding	bind	VBG
cord-333636-h2sg6shp	118	14	domain	domain	NN
cord-333636-h2sg6shp	118	15	among	among	IN
cord-333636-h2sg6shp	118	16	different	different	JJ
cord-333636-h2sg6shp	118	17	birnaviruses	birnaviruse	NNS
cord-333636-h2sg6shp	118	18	,	,	,
cord-333636-h2sg6shp	118	19	known	know	VBN
cord-333636-h2sg6shp	118	20	VP3	VP3	NNP
cord-333636-h2sg6shp	118	21	sequences	sequence	NNS
cord-333636-h2sg6shp	118	22	from	from	IN
cord-333636-h2sg6shp	118	23	representative	representative	JJ
cord-333636-h2sg6shp	118	24	members	member	NNS
cord-333636-h2sg6shp	118	25	of	of	IN
cord-333636-h2sg6shp	118	26	the	the	DT
cord-333636-h2sg6shp	118	27	family	family	NN
cord-333636-h2sg6shp	118	28	were	be	VBD
cord-333636-h2sg6shp	118	29	aligned	align	VBN
cord-333636-h2sg6shp	118	30	and	and	CC
cord-333636-h2sg6shp	118	31	compared	compare	VBN
cord-333636-h2sg6shp	118	32	using	use	VBG
cord-333636-h2sg6shp	118	33	the	the	DT
cord-333636-h2sg6shp	118	34	Clustal	Clustal	NNP
cord-333636-h2sg6shp	118	35	algorithm	algorithm	NN
cord-333636-h2sg6shp	118	36	[	[	-LRB-
cord-333636-h2sg6shp	118	37	41	41	CD
cord-333636-h2sg6shp	118	38	]	]	-RRB-
cord-333636-h2sg6shp	118	39	.	.	.
cord-333636-h2sg6shp	119	1	For	for	IN
cord-333636-h2sg6shp	119	2	alignment	alignment	NN
cord-333636-h2sg6shp	119	3	conditions	condition	NNS
cord-333636-h2sg6shp	119	4	,	,	,
cord-333636-h2sg6shp	119	5	the	the	DT
cord-333636-h2sg6shp	119	6	table	table	NN
cord-333636-h2sg6shp	119	7	of	of	IN
cord-333636-h2sg6shp	119	8	residue	residue	NN
cord-333636-h2sg6shp	119	9	weights	weight	NNS
cord-333636-h2sg6shp	119	10	used	use	VBN
cord-333636-h2sg6shp	119	11	was	be	VBD
cord-333636-h2sg6shp	119	12	PAM250	PAM250	NNP
cord-333636-h2sg6shp	119	13	with	with	IN
cord-333636-h2sg6shp	119	14	a	a	DT
cord-333636-h2sg6shp	119	15	gap	gap	NN
cord-333636-h2sg6shp	119	16	penalty	penalty	NN
cord-333636-h2sg6shp	119	17	of	of	IN
cord-333636-h2sg6shp	119	18	10	10	CD
cord-333636-h2sg6shp	119	19	[	[	-LRB-
cord-333636-h2sg6shp	119	20	12	12	CD
cord-333636-h2sg6shp	119	21	]	]	-RRB-
cord-333636-h2sg6shp	119	22	.	.	.
cord-333636-h2sg6shp	120	1	For	for	IN
cord-333636-h2sg6shp	120	2	this	this	DT
cord-333636-h2sg6shp	120	3	purpose	purpose	NN
cord-333636-h2sg6shp	120	4	,	,	,
cord-333636-h2sg6shp	120	5	the	the	DT
cord-333636-h2sg6shp	120	6	sequences	sequence	NNS
cord-333636-h2sg6shp	120	7	of	of	IN
cord-333636-h2sg6shp	120	8	VP3	VP3	NNP
cord-333636-h2sg6shp	120	9	proteins	protein	NNS
cord-333636-h2sg6shp	120	10	from	from	IN
cord-333636-h2sg6shp	120	11	different	different	JJ
cord-333636-h2sg6shp	120	12	species	specie	NNS
cord-333636-h2sg6shp	120	13	belonging	belong	VBG
cord-333636-h2sg6shp	120	14	to	to	IN
cord-333636-h2sg6shp	120	15	the	the	DT
cord-333636-h2sg6shp	120	16	avibirnavirus	avibirnavirus	NNP
cord-333636-h2sg6shp	120	17	,	,	,
cord-333636-h2sg6shp	120	18	aquabirnavirus	aquabirnavirus	NNP
cord-333636-h2sg6shp	120	19	,	,	,
cord-333636-h2sg6shp	120	20	and	and	CC
cord-333636-h2sg6shp	120	21	entomobirnavirus	entomobirnavirus	NNP
cord-333636-h2sg6shp	120	22	genera	genus	NNS
cord-333636-h2sg6shp	120	23	as	as	RB
cord-333636-h2sg6shp	120	24	well	well	RB
cord-333636-h2sg6shp	120	25	as	as	IN
cord-333636-h2sg6shp	120	26	to	to	IN
cord-333636-h2sg6shp	120	27	unclassified	unclassified	JJ
cord-333636-h2sg6shp	120	28	birnaviruses	birnaviruse	NNS
cord-333636-h2sg6shp	120	29	were	be	VBD
cord-333636-h2sg6shp	120	30	retrived	retrive	VBN
cord-333636-h2sg6shp	120	31	from	from	IN
cord-333636-h2sg6shp	120	32	GeneBank	GeneBank	NNP
cord-333636-h2sg6shp	120	33	.	.	.
cord-333636-h2sg6shp	121	1	For	for	IN
cord-333636-h2sg6shp	121	2	genus	genus	NN
cord-333636-h2sg6shp	121	3	avibirnavirus	avibirnavirus	NNP
cord-333636-h2sg6shp	121	4	,	,	,
cord-333636-h2sg6shp	121	5	IBDV	IBDV	NNP
cord-333636-h2sg6shp	121	6	virus	virus	NN
cord-333636-h2sg6shp	121	7	serotype	serotype	NN
cord-333636-h2sg6shp	121	8	1	1	CD
cord-333636-h2sg6shp	121	9	,	,	,
cord-333636-h2sg6shp	121	10	strain	strain	VB
cord-333636-h2sg6shp	121	11	Soroa	Soroa	NNP
cord-333636-h2sg6shp	121	12	(	(	-LRB-
cord-333636-h2sg6shp	121	13	AF140705.1	AF140705.1	NNP
cord-333636-h2sg6shp	121	14	)	)	-RRB-
cord-333636-h2sg6shp	121	15	,	,	,
cord-333636-h2sg6shp	121	16	strain	strain	VBP
cord-333636-h2sg6shp	121	17	OH	OH	NNP
cord-333636-h2sg6shp	121	18	(	(	-LRB-
cord-333636-h2sg6shp	121	19	U30818.1	U30818.1	NNP
cord-333636-h2sg6shp	121	20	)	)	-RRB-
cord-333636-h2sg6shp	121	21	,	,	,
cord-333636-h2sg6shp	121	22	strain	strain	VB
cord-333636-h2sg6shp	121	23	STC	STC	NNP
cord-333636-h2sg6shp	121	24	(	(	-LRB-
cord-333636-h2sg6shp	121	25	D00499	D00499	NNP
cord-333636-h2sg6shp	121	26	)	)	-RRB-
cord-333636-h2sg6shp	121	27	,	,	,
cord-333636-h2sg6shp	121	28	Cu-1wt	Cu-1wt	NNP
cord-333636-h2sg6shp	121	29	virus	virus	NN
cord-333636-h2sg6shp	121	30	(	(	-LRB-
cord-333636-h2sg6shp	121	31	AF362747.1	AF362747.1	NNP
cord-333636-h2sg6shp	121	32	)	)	-RRB-
cord-333636-h2sg6shp	121	33	,	,	,
cord-333636-h2sg6shp	121	34	and	and	CC
cord-333636-h2sg6shp	121	35	serotype	serotype	NN
cord-333636-h2sg6shp	121	36	2	2	CD
cord-333636-h2sg6shp	121	37	(	(	-LRB-
cord-333636-h2sg6shp	121	38	AF362773.1	AF362773.1	NNP
cord-333636-h2sg6shp	121	39	)	)	-RRB-
cord-333636-h2sg6shp	121	40	were	be	VBD
cord-333636-h2sg6shp	121	41	used	use	VBN
cord-333636-h2sg6shp	121	42	.	.	.
cord-333636-h2sg6shp	122	1	For	for	IN
cord-333636-h2sg6shp	122	2	aquabirnavirus	aquabirnavirus	NNP
cord-333636-h2sg6shp	122	3	genus	genus	NN
cord-333636-h2sg6shp	122	4	,	,	,
cord-333636-h2sg6shp	122	5	VP3	VP3	NNP
cord-333636-h2sg6shp	122	6	sequences	sequence	NNS
cord-333636-h2sg6shp	122	7	from	from	IN
cord-333636-h2sg6shp	122	8	IPNV	IPNV	NNP
cord-333636-h2sg6shp	122	9	strains	strain	VBZ
cord-333636-h2sg6shp	122	10	DRT	DRT	NNP
cord-333636-h2sg6shp	122	11	(	(	-LRB-
cord-333636-h2sg6shp	122	12	D26526.1	D26526.1	NNP
cord-333636-h2sg6shp	122	13	)	)	-RRB-
cord-333636-h2sg6shp	122	14	,	,	,
cord-333636-h2sg6shp	122	15	Jasper	Jasper	NNP
cord-333636-h2sg6shp	122	16	(	(	-LRB-
cord-333636-h2sg6shp	122	17	NC	NC	NNP
cord-333636-h2sg6shp	122	18	001915	001915	CD
cord-333636-h2sg6shp	122	19	)	)	-RRB-
cord-333636-h2sg6shp	122	20	,	,	,
cord-333636-h2sg6shp	122	21	SP	SP	NNP
cord-333636-h2sg6shp	122	22	(	(	-LRB-
cord-333636-h2sg6shp	122	23	AF342728.1	AF342728.1	NNP
cord-333636-h2sg6shp	122	24	)	)	-RRB-
cord-333636-h2sg6shp	122	25	,	,	,
cord-333636-h2sg6shp	122	26	and	and	CC
cord-333636-h2sg6shp	122	27	N1	N1	NNP
cord-333636-h2sg6shp	122	28	(	(	-LRB-
cord-333636-h2sg6shp	122	29	D00701	D00701	NNP
cord-333636-h2sg6shp	122	30	)	)	-RRB-
cord-333636-h2sg6shp	122	31	were	be	VBD
cord-333636-h2sg6shp	122	32	used	use	VBN
cord-333636-h2sg6shp	122	33	.	.	.
cord-333636-h2sg6shp	123	1	In	in	IN
cord-333636-h2sg6shp	123	2	the	the	DT
cord-333636-h2sg6shp	123	3	case	case	NN
cord-333636-h2sg6shp	123	4	of	of	IN
cord-333636-h2sg6shp	123	5	genus	genus	NN
cord-333636-h2sg6shp	123	6	entomobirnavirus	entomobirnavirus	NNP
cord-333636-h2sg6shp	123	7	,	,	,
cord-333636-h2sg6shp	123	8	the	the	DT
cord-333636-h2sg6shp	123	9	VP3	VP3	NNP
cord-333636-h2sg6shp	123	10	protein	protein	NN
cord-333636-h2sg6shp	123	11	sequence	sequence	NN
cord-333636-h2sg6shp	123	12	from	from	IN
cord-333636-h2sg6shp	123	13	Drosophila	Drosophila	NNP
cord-333636-h2sg6shp	123	14	X	X	NNP
cord-333636-h2sg6shp	123	15	virus	virus	NN
cord-333636-h2sg6shp	123	16	(	(	-LRB-
cord-333636-h2sg6shp	123	17	U60650	U60650	NNP
cord-333636-h2sg6shp	123	18	)	)	-RRB-
cord-333636-h2sg6shp	123	19	was	be	VBD
cord-333636-h2sg6shp	123	20	used	use	VBN
cord-333636-h2sg6shp	123	21	.	.	.
cord-333636-h2sg6shp	124	1	For	for	IN
cord-333636-h2sg6shp	124	2	unclassified	unclassified	JJ
cord-333636-h2sg6shp	124	3	birnaviruses	birnaviruse	NNS
cord-333636-h2sg6shp	124	4	,	,	,
cord-333636-h2sg6shp	124	5	the	the	DT
cord-333636-h2sg6shp	124	6	species	species	NNP
cord-333636-h2sg6shp	124	7	marine	marine	NNP
cord-333636-h2sg6shp	124	8	birnavirus	birnavirus	NNP
cord-333636-h2sg6shp	124	9	(	(	-LRB-
cord-333636-h2sg6shp	124	10	AB006783	AB006783	NNP
cord-333636-h2sg6shp	124	11	)	)	-RRB-
cord-333636-h2sg6shp	124	12	and	and	CC
cord-333636-h2sg6shp	124	13	yellowtale	yellowtale	NNP
cord-333636-h2sg6shp	124	14	ascites	ascites	NNP
cord-333636-h2sg6shp	124	15	virus	virus	NN
cord-333636-h2sg6shp	124	16	(	(	-LRB-
cord-333636-h2sg6shp	124	17	AB011440.1	AB011440.1	NNP
cord-333636-h2sg6shp	124	18	)	)	-RRB-
cord-333636-h2sg6shp	124	19	were	be	VBD
cord-333636-h2sg6shp	124	20	used	use	VBN
cord-333636-h2sg6shp	124	21	.	.	.
cord-333636-h2sg6shp	125	1	Previous	previous	JJ
cord-333636-h2sg6shp	125	2	studies	study	NNS
cord-333636-h2sg6shp	125	3	performed	perform	VBN
cord-333636-h2sg6shp	125	4	with	with	IN
cord-333636-h2sg6shp	125	5	the	the	DT
cord-333636-h2sg6shp	125	6	aquabirnavirus	aquabirnavirus	NNP
cord-333636-h2sg6shp	125	7	IPNV	IPNV	NNP
cord-333636-h2sg6shp	125	8	had	have	VBD
cord-333636-h2sg6shp	125	9	shown	show	VBN
cord-333636-h2sg6shp	125	10	the	the	DT
cord-333636-h2sg6shp	125	11	presence	presence	NN
cord-333636-h2sg6shp	125	12	of	of	IN
cord-333636-h2sg6shp	125	13	VP3	VP3	NNP
cord-333636-h2sg6shp	125	14	in	in	IN
cord-333636-h2sg6shp	125	15	ribonucleoprotein	ribonucleoprotein	NN
cord-333636-h2sg6shp	125	16	complexes	complex	NNS
cord-333636-h2sg6shp	125	17	isolated	isolate	VBN
cord-333636-h2sg6shp	125	18	from	from	IN
cord-333636-h2sg6shp	125	19	virus	virus	NN
cord-333636-h2sg6shp	125	20	particles	particle	NNS
cord-333636-h2sg6shp	125	21	[	[	-LRB-
cord-333636-h2sg6shp	125	22	22	22	CD
cord-333636-h2sg6shp	125	23	]	]	-RRB-
cord-333636-h2sg6shp	125	24	.	.	.
cord-333636-h2sg6shp	126	1	This	this	DT
cord-333636-h2sg6shp	126	2	observation	observation	NN
cord-333636-h2sg6shp	126	3	along	along	IN
cord-333636-h2sg6shp	126	4	with	with	IN
cord-333636-h2sg6shp	126	5	the	the	DT
cord-333636-h2sg6shp	126	6	fact	fact	NN
cord-333636-h2sg6shp	126	7	that	that	IN
cord-333636-h2sg6shp	126	8	VP3	VP3	NNP
cord-333636-h2sg6shp	126	9	coats	coat	VBZ
cord-333636-h2sg6shp	126	10	the	the	DT
cord-333636-h2sg6shp	126	11	inner	inner	JJ
cord-333636-h2sg6shp	126	12	surface	surface	NN
cord-333636-h2sg6shp	126	13	of	of	IN
cord-333636-h2sg6shp	126	14	the	the	DT
cord-333636-h2sg6shp	126	15	virus	virus	NN
cord-333636-h2sg6shp	126	16	particle	particle	NN
cord-333636-h2sg6shp	126	17	[	[	-LRB-
cord-333636-h2sg6shp	126	18	6	6	CD
cord-333636-h2sg6shp	126	19	,	,	,
cord-333636-h2sg6shp	126	20	9	9	CD
cord-333636-h2sg6shp	126	21	]	]	-RRB-
cord-333636-h2sg6shp	126	22	suggested	suggest	VBD
cord-333636-h2sg6shp	126	23	the	the	DT
cord-333636-h2sg6shp	126	24	possibility	possibility	NN
cord-333636-h2sg6shp	126	25	that	that	IN
cord-333636-h2sg6shp	126	26	VP3	VP3	NNP
cord-333636-h2sg6shp	126	27	might	may	MD
cord-333636-h2sg6shp	126	28	establish	establish	VB
cord-333636-h2sg6shp	126	29	direct	direct	JJ
cord-333636-h2sg6shp	126	30	interactions	interaction	NNS
cord-333636-h2sg6shp	126	31	with	with	IN
cord-333636-h2sg6shp	126	32	RNA	RNA	NNP
cord-333636-h2sg6shp	126	33	.	.	.
cord-333636-h2sg6shp	127	1	To	to	TO
cord-333636-h2sg6shp	127	2	study	study	VB
cord-333636-h2sg6shp	127	3	this	this	DT
cord-333636-h2sg6shp	127	4	possibility	possibility	NN
cord-333636-h2sg6shp	127	5	,	,	,
cord-333636-h2sg6shp	127	6	a	a	DT
cord-333636-h2sg6shp	127	7	recombinant	recombinant	JJ
cord-333636-h2sg6shp	127	8	histidine	histidine	NN
cord-333636-h2sg6shp	127	9	-	-	HYPH
cord-333636-h2sg6shp	127	10	tagged	tag	VBN
cord-333636-h2sg6shp	127	11	version	version	NN
cord-333636-h2sg6shp	127	12	of	of	IN
cord-333636-h2sg6shp	127	13	the	the	DT
cord-333636-h2sg6shp	127	14	IBDV	IBDV	NNP
cord-333636-h2sg6shp	127	15	VP3	VP3	NNP
cord-333636-h2sg6shp	127	16	was	be	VBD
cord-333636-h2sg6shp	127	17	produced	produce	VBN
cord-333636-h2sg6shp	127	18	and	and	CC
cord-333636-h2sg6shp	127	19	purified	purify	VBN
cord-333636-h2sg6shp	127	20	.	.	.
cord-333636-h2sg6shp	128	1	Purified	purified	JJ
cord-333636-h2sg6shp	128	2	VP3	vp3	NN
cord-333636-h2sg6shp	128	3	was	be	VBD
cord-333636-h2sg6shp	128	4	then	then	RB
cord-333636-h2sg6shp	128	5	used	use	VBN
cord-333636-h2sg6shp	128	6	to	to	TO
cord-333636-h2sg6shp	128	7	perform	perform	VB
cord-333636-h2sg6shp	128	8	a	a	DT
cord-333636-h2sg6shp	128	9	number	number	NN
cord-333636-h2sg6shp	128	10	of	of	IN
cord-333636-h2sg6shp	128	11	assays	assay	NNS
cord-333636-h2sg6shp	128	12	to	to	TO
cord-333636-h2sg6shp	128	13	unambiguously	unambiguously	RB
cord-333636-h2sg6shp	128	14	determine	determine	VB
cord-333636-h2sg6shp	128	15	its	-PRON-	PRP$
cord-333636-h2sg6shp	128	16	ability	ability	NN
cord-333636-h2sg6shp	128	17	to	to	TO
cord-333636-h2sg6shp	128	18	interact	interact	VB
cord-333636-h2sg6shp	128	19	with	with	IN
cord-333636-h2sg6shp	128	20	different	different	JJ
cord-333636-h2sg6shp	128	21	RNAs	rna	NNS
cord-333636-h2sg6shp	128	22	in	in	IN
cord-333636-h2sg6shp	128	23	the	the	DT
cord-333636-h2sg6shp	128	24	absence	absence	NN
cord-333636-h2sg6shp	128	25	of	of	IN
cord-333636-h2sg6shp	128	26	other	other	JJ
cord-333636-h2sg6shp	128	27	IBDV	IBDV	NNP
cord-333636-h2sg6shp	128	28	-	-	HYPH
cord-333636-h2sg6shp	128	29	encoded	encode	VBN
cord-333636-h2sg6shp	128	30	proteins	protein	NNS
cord-333636-h2sg6shp	128	31	.	.	.
cord-333636-h2sg6shp	129	1	Since	since	IN
cord-333636-h2sg6shp	129	2	preliminary	preliminary	JJ
cord-333636-h2sg6shp	129	3	results	result	NNS
cord-333636-h2sg6shp	129	4	indicated	indicate	VBD
cord-333636-h2sg6shp	129	5	that	that	IN
cord-333636-h2sg6shp	129	6	VP3	VP3	NNP
cord-333636-h2sg6shp	129	7	bound	bind	VBN
cord-333636-h2sg6shp	129	8	ssRNA	ssrna	RB
cord-333636-h2sg6shp	129	9	as	as	RB
cord-333636-h2sg6shp	129	10	well	well	RB
cord-333636-h2sg6shp	129	11	as	as	IN
cord-333636-h2sg6shp	129	12	dsRNA	dsrna	NN
cord-333636-h2sg6shp	129	13	,	,	,
cord-333636-h2sg6shp	129	14	ssRNA	ssrna	NN
cord-333636-h2sg6shp	129	15	probes	probe	NNS
cord-333636-h2sg6shp	129	16	were	be	VBD
cord-333636-h2sg6shp	129	17	used	use	VBN
cord-333636-h2sg6shp	129	18	for	for	IN
cord-333636-h2sg6shp	129	19	subsequent	subsequent	JJ
cord-333636-h2sg6shp	129	20	RNA	RNA	NNP
cord-333636-h2sg6shp	129	21	-	-	HYPH
cord-333636-h2sg6shp	129	22	binding	bind	VBG
cord-333636-h2sg6shp	129	23	analyses	analysis	NNS
cord-333636-h2sg6shp	129	24	.	.	.
cord-333636-h2sg6shp	130	1	In	in	IN
cord-333636-h2sg6shp	130	2	order	order	NN
cord-333636-h2sg6shp	130	3	to	to	TO
cord-333636-h2sg6shp	130	4	obtain	obtain	VB
cord-333636-h2sg6shp	130	5	sufficient	sufficient	JJ
cord-333636-h2sg6shp	130	6	purified	purify	VBN
cord-333636-h2sg6shp	130	7	VP3	vp3	NN
cord-333636-h2sg6shp	130	8	to	to	TO
cord-333636-h2sg6shp	130	9	perform	perform	VB
cord-333636-h2sg6shp	130	10	a	a	DT
cord-333636-h2sg6shp	130	11	series	series	NN
cord-333636-h2sg6shp	130	12	of	of	IN
cord-333636-h2sg6shp	130	13	biochemical	biochemical	JJ
cord-333636-h2sg6shp	130	14	experiments	experiment	NNS
cord-333636-h2sg6shp	130	15	described	describe	VBN
cord-333636-h2sg6shp	130	16	below	below	RB
cord-333636-h2sg6shp	130	17	,	,	,
cord-333636-h2sg6shp	130	18	VP3-Bac	vp3-bac	CD
cord-333636-h2sg6shp	130	19	,	,	,
cord-333636-h2sg6shp	130	20	a	a	DT
cord-333636-h2sg6shp	130	21	rBV	rbv	NN
cord-333636-h2sg6shp	130	22	,	,	,
cord-333636-h2sg6shp	130	23	was	be	VBD
cord-333636-h2sg6shp	130	24	generated	generate	VBN
cord-333636-h2sg6shp	130	25	.	.	.
cord-333636-h2sg6shp	131	1	This	this	DT
cord-333636-h2sg6shp	131	2	virus	virus	NN
cord-333636-h2sg6shp	131	3	expressed	express	VBD
cord-333636-h2sg6shp	131	4	a	a	DT
cord-333636-h2sg6shp	131	5	N	n	CD
cord-333636-h2sg6shp	131	6	-	-	HYPH
cord-333636-h2sg6shp	131	7	terminal	terminal	JJ
cord-333636-h2sg6shp	131	8	histidine	histidine	NN
cord-333636-h2sg6shp	131	9	-	-	HYPH
cord-333636-h2sg6shp	131	10	tagged	tag	VBN
cord-333636-h2sg6shp	131	11	version	version	NN
cord-333636-h2sg6shp	131	12	of	of	IN
cord-333636-h2sg6shp	131	13	VP3	VP3	NNP
cord-333636-h2sg6shp	131	14	under	under	IN
cord-333636-h2sg6shp	131	15	the	the	DT
cord-333636-h2sg6shp	131	16	control	control	NN
cord-333636-h2sg6shp	131	17	of	of	IN
cord-333636-h2sg6shp	131	18	the	the	DT
cord-333636-h2sg6shp	131	19	polyhedrin	polyhedrin	NN
cord-333636-h2sg6shp	131	20	promoter	promoter	NN
cord-333636-h2sg6shp	131	21	.	.	.
cord-333636-h2sg6shp	132	1	The	the	DT
cord-333636-h2sg6shp	132	2	ability	ability	NN
cord-333636-h2sg6shp	132	3	of	of	IN
cord-333636-h2sg6shp	132	4	VP3	VP3	NNP
cord-333636-h2sg6shp	132	5	to	to	TO
cord-333636-h2sg6shp	132	6	bind	bind	VB
cord-333636-h2sg6shp	132	7	ssRNA	ssRNA	VBG
cord-333636-h2sg6shp	132	8	was	be	VBD
cord-333636-h2sg6shp	132	9	initially	initially	RB
cord-333636-h2sg6shp	132	10	tested	test	VBN
cord-333636-h2sg6shp	132	11	by	by	IN
cord-333636-h2sg6shp	132	12	Northwestern	Northwestern	NNP
cord-333636-h2sg6shp	132	13	blotting	blot	VBG
cord-333636-h2sg6shp	132	14	using	use	VBG
cord-333636-h2sg6shp	132	15	total	total	JJ
cord-333636-h2sg6shp	132	16	cell	cell	NN
cord-333636-h2sg6shp	132	17	extracts	extract	NNS
cord-333636-h2sg6shp	132	18	from	from	IN
cord-333636-h2sg6shp	132	19	either	either	DT
cord-333636-h2sg6shp	132	20	WT	WT	NNP
cord-333636-h2sg6shp	132	21	baculovirus	baculovirus	NN
cord-333636-h2sg6shp	132	22	or	or	CC
cord-333636-h2sg6shp	132	23	VP3-Bac	vp3-bac	RB
cord-333636-h2sg6shp	132	24	-	-	HYPH
cord-333636-h2sg6shp	132	25	infected	infect	VBN
cord-333636-h2sg6shp	132	26	cells	cell	NNS
cord-333636-h2sg6shp	132	27	.	.	.
cord-333636-h2sg6shp	133	1	The	the	DT
cord-333636-h2sg6shp	133	2	assays	assay	NNS
cord-333636-h2sg6shp	133	3	were	be	VBD
cord-333636-h2sg6shp	133	4	carried	carry	VBN
cord-333636-h2sg6shp	133	5	out	out	RP
cord-333636-h2sg6shp	133	6	using	use	VBG
cord-333636-h2sg6shp	133	7	32	32	CD
cord-333636-h2sg6shp	133	8	P	p	NN
cord-333636-h2sg6shp	133	9	-	-	HYPH
cord-333636-h2sg6shp	133	10	labeled	label	VBN
cord-333636-h2sg6shp	133	11	ssRNA	ssrna	NN
cord-333636-h2sg6shp	133	12	probe	probe	NN
cord-333636-h2sg6shp	133	13	B.	B.	NNP
cord-333636-h2sg6shp	134	1	This	this	DT
cord-333636-h2sg6shp	134	2	probe	probe	NN
cord-333636-h2sg6shp	134	3	(	(	-LRB-
cord-333636-h2sg6shp	134	4	Fig	fig	NN
cord-333636-h2sg6shp	134	5	.	.	.
cord-333636-h2sg6shp	134	6	1	1	CD
cord-333636-h2sg6shp	134	7	)	)	-RRB-
cord-333636-h2sg6shp	134	8	,	,	,
cord-333636-h2sg6shp	134	9	VP3	VP3	NNP
cord-333636-h2sg6shp	134	10	was	be	VBD
cord-333636-h2sg6shp	134	11	expressed	express	VBN
cord-333636-h2sg6shp	134	12	at	at	IN
cord-333636-h2sg6shp	134	13	high	high	JJ
cord-333636-h2sg6shp	134	14	levels	level	NNS
cord-333636-h2sg6shp	134	15	in	in	IN
cord-333636-h2sg6shp	134	16	cells	cell	NNS
cord-333636-h2sg6shp	134	17	infected	infect	VBN
cord-333636-h2sg6shp	134	18	with	with	IN
cord-333636-h2sg6shp	134	19	VP3-Bac	vp3-bac	CD
cord-333636-h2sg6shp	134	20	.	.	.
cord-333636-h2sg6shp	135	1	A	a	DT
cord-333636-h2sg6shp	135	2	significant	significant	JJ
cord-333636-h2sg6shp	135	3	binding	binding	NN
cord-333636-h2sg6shp	135	4	with	with	IN
cord-333636-h2sg6shp	135	5	ssRNA	ssRNA	``
cord-333636-h2sg6shp	135	6	probe	probe	NN
cord-333636-h2sg6shp	135	7	B	B	NNP
cord-333636-h2sg6shp	135	8	was	be	VBD
cord-333636-h2sg6shp	135	9	observed	observe	VBN
cord-333636-h2sg6shp	135	10	by	by	IN
cord-333636-h2sg6shp	135	11	the	the	DT
cord-333636-h2sg6shp	135	12	expressed	express	VBN
cord-333636-h2sg6shp	135	13	VP3	VP3	NNP
cord-333636-h2sg6shp	135	14	in	in	IN
cord-333636-h2sg6shp	135	15	VP3-Bac	vp3-bac	CD
cord-333636-h2sg6shp	135	16	infected	infected	JJ
cord-333636-h2sg6shp	135	17	cell	cell	NN
cord-333636-h2sg6shp	135	18	extracts	extract	NNS
cord-333636-h2sg6shp	135	19	(	(	-LRB-
cord-333636-h2sg6shp	135	20	Fig	Fig	NNP
cord-333636-h2sg6shp	135	21	.	.	.
cord-333636-h2sg6shp	135	22	1	1	CD
cord-333636-h2sg6shp	135	23	)	)	-RRB-
cord-333636-h2sg6shp	135	24	.	.	.
cord-333636-h2sg6shp	136	1	A	a	DT
cord-333636-h2sg6shp	136	2	weak	weak	JJ
cord-333636-h2sg6shp	136	3	but	but	CC
cord-333636-h2sg6shp	136	4	significant	significant	JJ
cord-333636-h2sg6shp	136	5	band	band	NN
cord-333636-h2sg6shp	136	6	of	of	IN
cord-333636-h2sg6shp	136	7	bound	bind	VBN
cord-333636-h2sg6shp	136	8	probe	probe	NN
cord-333636-h2sg6shp	136	9	was	be	VBD
cord-333636-h2sg6shp	136	10	observed	observe	VBN
cord-333636-h2sg6shp	136	11	also	also	RB
cord-333636-h2sg6shp	136	12	in	in	IN
cord-333636-h2sg6shp	136	13	WT	WT	NNP
cord-333636-h2sg6shp	136	14	-	-	HYPH
cord-333636-h2sg6shp	136	15	infected	infect	VBN
cord-333636-h2sg6shp	136	16	cells	cell	NNS
cord-333636-h2sg6shp	136	17	,	,	,
cord-333636-h2sg6shp	136	18	at	at	IN
cord-333636-h2sg6shp	136	19	the	the	DT
cord-333636-h2sg6shp	136	20	same	same	JJ
cord-333636-h2sg6shp	136	21	position	position	NN
cord-333636-h2sg6shp	136	22	as	as	IN
cord-333636-h2sg6shp	136	23	IBDV	IBDV	NNP
cord-333636-h2sg6shp	136	24	VP3	VP3	NNP
cord-333636-h2sg6shp	136	25	.	.	.
cord-333636-h2sg6shp	137	1	This	this	DT
cord-333636-h2sg6shp	137	2	band	band	NN
cord-333636-h2sg6shp	137	3	accounted	account	VBD
cord-333636-h2sg6shp	137	4	for	for	IN
cord-333636-h2sg6shp	137	5	a	a	DT
cord-333636-h2sg6shp	137	6	5	5	CD
cord-333636-h2sg6shp	137	7	%	%	NN
cord-333636-h2sg6shp	137	8	of	of	IN
cord-333636-h2sg6shp	137	9	total	total	JJ
cord-333636-h2sg6shp	137	10	bound	bind	VBN
cord-333636-h2sg6shp	137	11	RNA	RNA	NNP
cord-333636-h2sg6shp	137	12	probe	probe	NN
cord-333636-h2sg6shp	137	13	when	when	WRB
cord-333636-h2sg6shp	137	14	compared	compare	VBN
cord-333636-h2sg6shp	137	15	to	to	IN
cord-333636-h2sg6shp	137	16	the	the	DT
cord-333636-h2sg6shp	137	17	cell	cell	NN
cord-333636-h2sg6shp	137	18	lysate	lysate	NN
cord-333636-h2sg6shp	137	19	containing	contain	VBG
cord-333636-h2sg6shp	137	20	VP3	VP3	NNP
cord-333636-h2sg6shp	137	21	,	,	,
cord-333636-h2sg6shp	137	22	as	as	IN
cord-333636-h2sg6shp	137	23	determined	determine	VBN
cord-333636-h2sg6shp	137	24	by	by	IN
cord-333636-h2sg6shp	137	25	densitometric	densitometric	JJ
cord-333636-h2sg6shp	137	26	analysis	analysis	NN
cord-333636-h2sg6shp	137	27	.	.	.
cord-333636-h2sg6shp	138	1	Western	western	JJ
cord-333636-h2sg6shp	138	2	blot	blot	NN
cord-333636-h2sg6shp	138	3	analysis	analysis	NN
cord-333636-h2sg6shp	138	4	with	with	IN
cord-333636-h2sg6shp	138	5	a	a	DT
cord-333636-h2sg6shp	138	6	VP3specific	VP3specific	NNP
cord-333636-h2sg6shp	138	7	MAb	MAb	NNS
cord-333636-h2sg6shp	138	8	confirmed	confirm	VBD
cord-333636-h2sg6shp	138	9	that	that	IN
cord-333636-h2sg6shp	138	10	the	the	DT
cord-333636-h2sg6shp	138	11	RNA	RNA	NNP
cord-333636-h2sg6shp	138	12	-	-	HYPH
cord-333636-h2sg6shp	138	13	binding	bind	VBG
cord-333636-h2sg6shp	138	14	protein	protein	NN
cord-333636-h2sg6shp	138	15	expressed	express	VBN
cord-333636-h2sg6shp	138	16	after	after	IN
cord-333636-h2sg6shp	138	17	infection	infection	NN
cord-333636-h2sg6shp	138	18	with	with	IN
cord-333636-h2sg6shp	138	19	VP3-Bac	vp3-bac	CD
cord-333636-h2sg6shp	138	20	was	be	VBD
cord-333636-h2sg6shp	138	21	in	in	IN
cord-333636-h2sg6shp	138	22	fact	fact	NN
cord-333636-h2sg6shp	139	1	VP3	VP3	NNP
cord-333636-h2sg6shp	139	2	protein	protein	NN
cord-333636-h2sg6shp	139	3	(	(	-LRB-
cord-333636-h2sg6shp	139	4	Fig	Fig	NNP
cord-333636-h2sg6shp	139	5	.	.	.
cord-333636-h2sg6shp	139	6	1	1	CD
cord-333636-h2sg6shp	139	7	)	)	-RRB-
cord-333636-h2sg6shp	139	8	.	.	.
cord-333636-h2sg6shp	140	1	The	the	DT
cord-333636-h2sg6shp	140	2	RNA	RNA	NNP
cord-333636-h2sg6shp	140	3	-	-	HYPH
cord-333636-h2sg6shp	140	4	binding	bind	VBG
cord-333636-h2sg6shp	140	5	protein	protein	NN
cord-333636-h2sg6shp	140	6	present	present	NN
cord-333636-h2sg6shp	140	7	in	in	IN
cord-333636-h2sg6shp	140	8	cells	cell	NNS
cord-333636-h2sg6shp	140	9	infected	infect	VBN
cord-333636-h2sg6shp	140	10	with	with	IN
cord-333636-h2sg6shp	140	11	WT	WT	NNP
cord-333636-h2sg6shp	140	12	baculovirus	baculovirus	NNP
cord-333636-h2sg6shp	140	13	did	do	VBD
cord-333636-h2sg6shp	140	14	not	not	RB
cord-333636-h2sg6shp	140	15	react	react	VB
cord-333636-h2sg6shp	140	16	with	with	IN
cord-333636-h2sg6shp	140	17	VP3-specific	VP3-specific	NNP
cord-333636-h2sg6shp	140	18	antibodies	antibody	NNS
cord-333636-h2sg6shp	140	19	,	,	,
cord-333636-h2sg6shp	140	20	suggesting	suggest	VBG
cord-333636-h2sg6shp	140	21	that	that	IN
cord-333636-h2sg6shp	140	22	this	this	DT
cord-333636-h2sg6shp	140	23	protein	protein	NN
cord-333636-h2sg6shp	140	24	was	be	VBD
cord-333636-h2sg6shp	140	25	either	either	CC
cord-333636-h2sg6shp	140	26	cellular	cellular	JJ
cord-333636-h2sg6shp	140	27	or	or	CC
cord-333636-h2sg6shp	140	28	viral	viral	JJ
cord-333636-h2sg6shp	140	29	,	,	,
cord-333636-h2sg6shp	140	30	but	but	CC
cord-333636-h2sg6shp	140	31	not	not	RB
cord-333636-h2sg6shp	140	32	VP3	VP3	NNP
cord-333636-h2sg6shp	140	33	.	.	.
cord-333636-h2sg6shp	141	1	These	these	DT
cord-333636-h2sg6shp	141	2	results	result	NNS
cord-333636-h2sg6shp	141	3	also	also	RB
cord-333636-h2sg6shp	141	4	showed	show	VBD
cord-333636-h2sg6shp	141	5	that	that	IN
cord-333636-h2sg6shp	141	6	monomers	monomer	NNS
cord-333636-h2sg6shp	141	7	of	of	IN
cord-333636-h2sg6shp	141	8	VP3	VP3	NNP
cord-333636-h2sg6shp	141	9	fixed	fix	VBN
cord-333636-h2sg6shp	141	10	to	to	IN
cord-333636-h2sg6shp	141	11	nitrocellulose	nitrocellulose	NN
cord-333636-h2sg6shp	141	12	filters	filter	NNS
cord-333636-h2sg6shp	141	13	bound	bind	VBN
cord-333636-h2sg6shp	141	14	the	the	DT
cord-333636-h2sg6shp	141	15	ssRNA	ssRNA	``
cord-333636-h2sg6shp	141	16	probe	probe	NN
cord-333636-h2sg6shp	141	17	B.	B.	NNP
cord-333636-h2sg6shp	142	1	To	to	TO
cord-333636-h2sg6shp	142	2	further	further	RB
cord-333636-h2sg6shp	142	3	characterize	characterize	VB
cord-333636-h2sg6shp	142	4	the	the	DT
cord-333636-h2sg6shp	142	5	binding	bind	VBG
cord-333636-h2sg6shp	142	6	properties	property	NNS
cord-333636-h2sg6shp	142	7	of	of	IN
cord-333636-h2sg6shp	142	8	purified	purify	VBN
cord-333636-h2sg6shp	142	9	VP3	VP3	NNP
cord-333636-h2sg6shp	142	10	to	to	IN
cord-333636-h2sg6shp	142	11	ssRNA	ssrna	NN
cord-333636-h2sg6shp	142	12	,	,	,
cord-333636-h2sg6shp	142	13	EMSA	EMSA	NNP
cord-333636-h2sg6shp	142	14	assays	assay	NNS
cord-333636-h2sg6shp	142	15	were	be	VBD
cord-333636-h2sg6shp	142	16	performed	perform	VBN
cord-333636-h2sg6shp	142	17	with	with	IN
cord-333636-h2sg6shp	142	18	32	32	CD
cord-333636-h2sg6shp	142	19	P	p	NN
cord-333636-h2sg6shp	142	20	-	-	HYPH
cord-333636-h2sg6shp	142	21	labeled	label	VBN
cord-333636-h2sg6shp	142	22	probe	probe	NN
cord-333636-h2sg6shp	142	23	B.	B.	NNP
cord-333636-h2sg6shp	142	24	Purified	Purified	NNP
cord-333636-h2sg6shp	142	25	VP3	VP3	NNP
cord-333636-h2sg6shp	142	26	was	be	VBD
cord-333636-h2sg6shp	142	27	used	use	VBN
cord-333636-h2sg6shp	142	28	for	for	IN
cord-333636-h2sg6shp	142	29	these	these	DT
cord-333636-h2sg6shp	142	30	studies	study	NNS
cord-333636-h2sg6shp	142	31	instead	instead	RB
cord-333636-h2sg6shp	142	32	of	of	IN
cord-333636-h2sg6shp	142	33	total	total	JJ
cord-333636-h2sg6shp	142	34	cellular	cellular	JJ
cord-333636-h2sg6shp	142	35	extracts	extract	NNS
cord-333636-h2sg6shp	142	36	due	due	IN
cord-333636-h2sg6shp	142	37	to	to	IN
cord-333636-h2sg6shp	142	38	the	the	DT
cord-333636-h2sg6shp	142	39	existence	existence	NN
cord-333636-h2sg6shp	142	40	of	of	IN
cord-333636-h2sg6shp	142	41	an	an	DT
cord-333636-h2sg6shp	142	42	RNA	RNA	NNP
cord-333636-h2sg6shp	142	43	-	-	HYPH
cord-333636-h2sg6shp	142	44	binding	bind	VBG
cord-333636-h2sg6shp	142	45	protein	protein	NN
cord-333636-h2sg6shp	142	46	in	in	IN
cord-333636-h2sg6shp	142	47	control	control	NN
cord-333636-h2sg6shp	142	48	cell	cell	NN
cord-333636-h2sg6shp	142	49	extracts	extract	NNS
cord-333636-h2sg6shp	142	50	that	that	WDT
cord-333636-h2sg6shp	142	51	might	may	MD
cord-333636-h2sg6shp	142	52	interfere	interfere	VB
cord-333636-h2sg6shp	142	53	with	with	IN
cord-333636-h2sg6shp	142	54	the	the	DT
cord-333636-h2sg6shp	142	55	results	result	NNS
cord-333636-h2sg6shp	142	56	(	(	-LRB-
cord-333636-h2sg6shp	142	57	Fig	fig	NN
cord-333636-h2sg6shp	142	58	.	.	NNP
cord-333636-h2sg6shp	142	59	1	1	CD
cord-333636-h2sg6shp	142	60	)	)	-RRB-
cord-333636-h2sg6shp	142	61	.	.	.
cord-333636-h2sg6shp	143	1	A	a	DT
cord-333636-h2sg6shp	143	2	series	series	NN
cord-333636-h2sg6shp	143	3	of	of	IN
cord-333636-h2sg6shp	143	4	shifted	shift	VBN
cord-333636-h2sg6shp	143	5	bands	band	NNS
cord-333636-h2sg6shp	143	6	of	of	IN
cord-333636-h2sg6shp	143	7	RNA	rna	NN
cord-333636-h2sg6shp	143	8	-	-	HYPH
cord-333636-h2sg6shp	143	9	protein	protein	NN
cord-333636-h2sg6shp	143	10	complexes	complex	NNS
cord-333636-h2sg6shp	143	11	were	be	VBD
cord-333636-h2sg6shp	143	12	visualized	visualize	VBN
cord-333636-h2sg6shp	143	13	after	after	IN
cord-333636-h2sg6shp	143	14	incubation	incubation	NN
cord-333636-h2sg6shp	143	15	of	of	IN
cord-333636-h2sg6shp	143	16	purified	purify	VBN
cord-333636-h2sg6shp	143	17	VP3	vp3	NN
cord-333636-h2sg6shp	143	18	with	with	IN
cord-333636-h2sg6shp	143	19	radioactive	radioactive	JJ
cord-333636-h2sg6shp	143	20	probe	probe	NN
cord-333636-h2sg6shp	143	21	B	b	NN
cord-333636-h2sg6shp	143	22	(	(	-LRB-
cord-333636-h2sg6shp	143	23	Fig	Fig	NNP
cord-333636-h2sg6shp	143	24	.	.	NNP
cord-333636-h2sg6shp	143	25	2	2	CD
cord-333636-h2sg6shp	143	26	)	)	-RRB-
cord-333636-h2sg6shp	143	27	.	.	.
cord-333636-h2sg6shp	144	1	The	the	DT
cord-333636-h2sg6shp	144	2	intensity	intensity	NN
cord-333636-h2sg6shp	144	3	of	of	IN
cord-333636-h2sg6shp	144	4	these	these	DT
cord-333636-h2sg6shp	144	5	bands	band	NNS
cord-333636-h2sg6shp	144	6	was	be	VBD
cord-333636-h2sg6shp	144	7	directly	directly	RB
cord-333636-h2sg6shp	144	8	dependent	dependent	JJ
cord-333636-h2sg6shp	144	9	upon	upon	IN
cord-333636-h2sg6shp	144	10	the	the	DT
cord-333636-h2sg6shp	144	11	amount	amount	NN
cord-333636-h2sg6shp	144	12	of	of	IN
cord-333636-h2sg6shp	144	13	VP3	VP3	NNP
cord-333636-h2sg6shp	144	14	used	use	VBN
cord-333636-h2sg6shp	144	15	in	in	IN
cord-333636-h2sg6shp	144	16	the	the	DT
cord-333636-h2sg6shp	144	17	binding	binding	JJ
cord-333636-h2sg6shp	144	18	reaction	reaction	NN
cord-333636-h2sg6shp	144	19	mixtures	mixture	NNS
cord-333636-h2sg6shp	144	20	(	(	-LRB-
cord-333636-h2sg6shp	144	21	Fig	Fig	NNP
cord-333636-h2sg6shp	144	22	.	.	.
cord-333636-h2sg6shp	144	23	2A	2a	CD
cord-333636-h2sg6shp	144	24	)	)	-RRB-
cord-333636-h2sg6shp	144	25	,	,	,
cord-333636-h2sg6shp	144	26	reaching	reach	VBG
cord-333636-h2sg6shp	144	27	a	a	DT
cord-333636-h2sg6shp	144	28	plateau	plateau	NN
cord-333636-h2sg6shp	144	29	above	above	IN
cord-333636-h2sg6shp	144	30	a	a	DT
cord-333636-h2sg6shp	144	31	VP3	vp3	JJ
cord-333636-h2sg6shp	144	32	concentration	concentration	NN
cord-333636-h2sg6shp	144	33	of	of	IN
cord-333636-h2sg6shp	144	34	6	6	CD
cord-333636-h2sg6shp	144	35	×	×	NN
cord-333636-h2sg6shp	144	36	10	10	CD
cord-333636-h2sg6shp	145	1	−4	−4	CD
cord-333636-h2sg6shp	146	1	mM.	mM.	NNP
cord-333636-h2sg6shp	146	2	Results	Results	NNP
cord-333636-h2sg6shp	146	3	were	be	VBD
cord-333636-h2sg6shp	146	4	reproducibly	reproducibly	RB
cord-333636-h2sg6shp	146	5	observed	observe	VBN
cord-333636-h2sg6shp	146	6	in	in	IN
cord-333636-h2sg6shp	146	7	all	all	DT
cord-333636-h2sg6shp	146	8	experiments	experiment	NNS
cord-333636-h2sg6shp	146	9	.	.	.
cord-333636-h2sg6shp	147	1	No	no	DT
cord-333636-h2sg6shp	147	2	shifted	shift	VBN
cord-333636-h2sg6shp	147	3	bands	band	NNS
cord-333636-h2sg6shp	147	4	were	be	VBD
cord-333636-h2sg6shp	147	5	observed	observe	VBN
cord-333636-h2sg6shp	147	6	when	when	WRB
cord-333636-h2sg6shp	147	7	relatively	relatively	RB
cord-333636-h2sg6shp	147	8	high	high	JJ
cord-333636-h2sg6shp	147	9	amounts	amount	NNS
cord-333636-h2sg6shp	147	10	of	of	IN
cord-333636-h2sg6shp	147	11	BSA	BSA	NNP
cord-333636-h2sg6shp	147	12	were	be	VBD
cord-333636-h2sg6shp	147	13	used	use	VBN
cord-333636-h2sg6shp	147	14	in	in	IN
cord-333636-h2sg6shp	147	15	the	the	DT
cord-333636-h2sg6shp	147	16	binding	bind	VBG
cord-333636-h2sg6shp	147	17	mixtures	mixture	NNS
cord-333636-h2sg6shp	147	18	,	,	,
cord-333636-h2sg6shp	147	19	as	as	IN
cord-333636-h2sg6shp	147	20	expected	expect	VBN
cord-333636-h2sg6shp	147	21	(	(	-LRB-
cord-333636-h2sg6shp	147	22	Fig	Fig	NNP
cord-333636-h2sg6shp	147	23	.	.	NNP
cord-333636-h2sg6shp	147	24	2	2	CD
cord-333636-h2sg6shp	147	25	)	)	-RRB-
cord-333636-h2sg6shp	147	26	.	.	.
cord-333636-h2sg6shp	148	1	These	these	DT
cord-333636-h2sg6shp	148	2	results	result	NNS
cord-333636-h2sg6shp	148	3	further	further	RB
cord-333636-h2sg6shp	148	4	showed	show	VBD
cord-333636-h2sg6shp	148	5	that	that	IN
cord-333636-h2sg6shp	148	6	baculovirus	baculovirus	NN
cord-333636-h2sg6shp	148	7	-	-	HYPH
cord-333636-h2sg6shp	148	8	expressed	express	VBN
cord-333636-h2sg6shp	148	9	VP3	VP3	NNP
cord-333636-h2sg6shp	148	10	bound	bind	VBN
cord-333636-h2sg6shp	148	11	ssRNA	ssrna	NN
cord-333636-h2sg6shp	148	12	probe	probe	NN
cord-333636-h2sg6shp	148	13	B.	B.	NNP
cord-333636-h2sg6shp	149	1	Since	since	IN
cord-333636-h2sg6shp	149	2	pure	pure	JJ
cord-333636-h2sg6shp	149	3	VP3	vp3	NN
cord-333636-h2sg6shp	149	4	was	be	VBD
cord-333636-h2sg6shp	149	5	used	use	VBN
cord-333636-h2sg6shp	149	6	for	for	IN
cord-333636-h2sg6shp	149	7	the	the	DT
cord-333636-h2sg6shp	149	8	studies	study	NNS
cord-333636-h2sg6shp	149	9	,	,	,
cord-333636-h2sg6shp	149	10	these	these	DT
cord-333636-h2sg6shp	149	11	results	result	NNS
cord-333636-h2sg6shp	149	12	also	also	RB
cord-333636-h2sg6shp	149	13	showed	show	VBD
cord-333636-h2sg6shp	149	14	that	that	IN
cord-333636-h2sg6shp	149	15	VP3	VP3	NNP
cord-333636-h2sg6shp	149	16	did	do	VBD
cord-333636-h2sg6shp	149	17	not	not	RB
cord-333636-h2sg6shp	149	18	require	require	VB
cord-333636-h2sg6shp	149	19	the	the	DT
cord-333636-h2sg6shp	149	20	presence	presence	NN
cord-333636-h2sg6shp	149	21	of	of	IN
cord-333636-h2sg6shp	149	22	other	other	JJ
cord-333636-h2sg6shp	149	23	virus	virus	NN
cord-333636-h2sg6shp	149	24	or	or	CC
cord-333636-h2sg6shp	149	25	cellular	cellular	JJ
cord-333636-h2sg6shp	149	26	proteins	protein	NNS
cord-333636-h2sg6shp	149	27	to	to	TO
cord-333636-h2sg6shp	149	28	bind	bind	VB
cord-333636-h2sg6shp	149	29	ssRNA	ssRNA	NNS
cord-333636-h2sg6shp	149	30	.	.	.
cord-333636-h2sg6shp	150	1	To	to	TO
cord-333636-h2sg6shp	150	2	confirm	confirm	VB
cord-333636-h2sg6shp	150	3	that	that	IN
cord-333636-h2sg6shp	150	4	the	the	DT
cord-333636-h2sg6shp	150	5	shifted	shift	VBN
cord-333636-h2sg6shp	150	6	bands	band	NNS
cord-333636-h2sg6shp	150	7	corresponded	correspond	VBD
cord-333636-h2sg6shp	150	8	to	to	IN
cord-333636-h2sg6shp	150	9	ribonucleoprotein	ribonucleoprotein	NN
cord-333636-h2sg6shp	150	10	complexes	complex	NNS
cord-333636-h2sg6shp	150	11	containing	contain	VBG
cord-333636-h2sg6shp	150	12	VP3	VP3	NNP
cord-333636-h2sg6shp	150	13	and	and	CC
cord-333636-h2sg6shp	150	14	not	not	RB
cord-333636-h2sg6shp	150	15	to	to	TO
cord-333636-h2sg6shp	150	16	aggregated	aggregate	VBN
cord-333636-h2sg6shp	150	17	RNA	RNA	NNP
cord-333636-h2sg6shp	150	18	,	,	,
cord-333636-h2sg6shp	150	19	supershift	supershift	NN
cord-333636-h2sg6shp	150	20	assays	assay	NNS
cord-333636-h2sg6shp	150	21	were	be	VBD
cord-333636-h2sg6shp	150	22	performed	perform	VBN
cord-333636-h2sg6shp	150	23	using	use	VBG
cord-333636-h2sg6shp	150	24	purified	purify	VBN
cord-333636-h2sg6shp	150	25	VP3	VP3	NNP
cord-333636-h2sg6shp	150	26	protein	protein	NN
cord-333636-h2sg6shp	150	27	-	-	HYPH
cord-333636-h2sg6shp	150	28	specific	specific	JJ
cord-333636-h2sg6shp	150	29	MAbs	MAbs	NNP
cord-333636-h2sg6shp	150	30	(	(	-LRB-
cord-333636-h2sg6shp	150	31	Fig	Fig	NNP
cord-333636-h2sg6shp	150	32	.	.	.
cord-333636-h2sg6shp	150	33	2B	2b	CD
cord-333636-h2sg6shp	150	34	)	)	-RRB-
cord-333636-h2sg6shp	150	35	.	.	.
cord-333636-h2sg6shp	151	1	When	when	WRB
cord-333636-h2sg6shp	151	2	BSA	BSA	NNP
cord-333636-h2sg6shp	151	3	was	be	VBD
cord-333636-h2sg6shp	151	4	used	use	VBN
cord-333636-h2sg6shp	151	5	as	as	IN
cord-333636-h2sg6shp	151	6	a	a	DT
cord-333636-h2sg6shp	151	7	control	control	NN
cord-333636-h2sg6shp	151	8	at	at	IN
cord-333636-h2sg6shp	151	9	high	high	JJ
cord-333636-h2sg6shp	151	10	amounts	amount	NNS
cord-333636-h2sg6shp	151	11	,	,	,
cord-333636-h2sg6shp	151	12	no	no	DT
cord-333636-h2sg6shp	151	13	shifted	shift	VBN
cord-333636-h2sg6shp	151	14	bands	band	NNS
cord-333636-h2sg6shp	151	15	were	be	VBD
cord-333636-h2sg6shp	151	16	observed	observe	VBN
cord-333636-h2sg6shp	151	17	(	(	-LRB-
cord-333636-h2sg6shp	151	18	Fig	Fig	NNP
cord-333636-h2sg6shp	151	19	.	.	.
cord-333636-h2sg6shp	151	20	2B	2b	CD
cord-333636-h2sg6shp	151	21	)	)	-RRB-
cord-333636-h2sg6shp	151	22	.	.	.
cord-333636-h2sg6shp	152	1	In	in	IN
cord-333636-h2sg6shp	152	2	constrast	constrast	NN
cord-333636-h2sg6shp	152	3	,	,	,
cord-333636-h2sg6shp	152	4	VP3	VP3	NNP
cord-333636-h2sg6shp	152	5	alone	alone	RB
cord-333636-h2sg6shp	152	6	or	or	CC
cord-333636-h2sg6shp	152	7	in	in	IN
cord-333636-h2sg6shp	152	8	the	the	DT
cord-333636-h2sg6shp	152	9	presence	presence	NN
cord-333636-h2sg6shp	152	10	of	of	IN
cord-333636-h2sg6shp	152	11	a	a	DT
cord-333636-h2sg6shp	152	12	non	non	JJ
cord-333636-h2sg6shp	152	13	-	-	JJ
cord-333636-h2sg6shp	152	14	specific	specific	JJ
cord-333636-h2sg6shp	152	15	IgG	igg	NN
cord-333636-h2sg6shp	152	16	formed	form	VBN
cord-333636-h2sg6shp	152	17	at	at	RB
cord-333636-h2sg6shp	152	18	least	least	RBS
cord-333636-h2sg6shp	152	19	three	three	CD
cord-333636-h2sg6shp	152	20	ribonucleoprotein	ribonucleoprotein	NN
cord-333636-h2sg6shp	152	21	complexes	complex	NNS
cord-333636-h2sg6shp	152	22	of	of	IN
cord-333636-h2sg6shp	152	23	different	different	JJ
cord-333636-h2sg6shp	152	24	electrophoretic	electrophoretic	JJ
cord-333636-h2sg6shp	152	25	mobilities	mobility	NNS
cord-333636-h2sg6shp	152	26	(	(	-LRB-
cord-333636-h2sg6shp	152	27	Fig	Fig	NNP
cord-333636-h2sg6shp	152	28	.	.	.
cord-333636-h2sg6shp	152	29	2B	2b	CD
cord-333636-h2sg6shp	152	30	)	)	-RRB-
cord-333636-h2sg6shp	152	31	.	.	.
cord-333636-h2sg6shp	153	1	The	the	DT
cord-333636-h2sg6shp	153	2	complex	complex	NN
cord-333636-h2sg6shp	153	3	of	of	IN
cord-333636-h2sg6shp	153	4	higher	high	JJR
cord-333636-h2sg6shp	153	5	electrophoretic	electrophoretic	JJ
cord-333636-h2sg6shp	153	6	mobility	mobility	NN
cord-333636-h2sg6shp	153	7	probably	probably	RB
cord-333636-h2sg6shp	153	8	corresponded	correspond	VBD
cord-333636-h2sg6shp	153	9	to	to	IN
cord-333636-h2sg6shp	153	10	monomer	monomer	NN
cord-333636-h2sg6shp	153	11	VP3	VP3	NNP
cord-333636-h2sg6shp	153	12	molecules	molecule	NNS
cord-333636-h2sg6shp	153	13	binding	bind	VBG
cord-333636-h2sg6shp	153	14	to	to	IN
cord-333636-h2sg6shp	153	15	the	the	DT
cord-333636-h2sg6shp	153	16	RNA	RNA	NNP
cord-333636-h2sg6shp	153	17	probe	probe	NN
cord-333636-h2sg6shp	153	18	,	,	,
cord-333636-h2sg6shp	153	19	since	since	IN
cord-333636-h2sg6shp	153	20	when	when	WRB
cord-333636-h2sg6shp	153	21	low	low	JJ
cord-333636-h2sg6shp	153	22	amounts	amount	NNS
cord-333636-h2sg6shp	153	23	of	of	IN
cord-333636-h2sg6shp	153	24	VP3	vp3	NN
cord-333636-h2sg6shp	153	25	protein	protein	NN
cord-333636-h2sg6shp	153	26	were	be	VBD
cord-333636-h2sg6shp	153	27	used	use	VBN
cord-333636-h2sg6shp	153	28	in	in	IN
cord-333636-h2sg6shp	153	29	binding	bind	VBG
cord-333636-h2sg6shp	153	30	reactions	reaction	NNS
cord-333636-h2sg6shp	153	31	this	this	DT
cord-333636-h2sg6shp	153	32	was	be	VBD
cord-333636-h2sg6shp	153	33	the	the	DT
cord-333636-h2sg6shp	153	34	only	only	RB
cord-333636-h2sg6shp	153	35	shifted	shift	VBN
cord-333636-h2sg6shp	153	36	band	band	NN
cord-333636-h2sg6shp	153	37	(	(	-LRB-
cord-333636-h2sg6shp	153	38	Fig	Fig	NNP
cord-333636-h2sg6shp	153	39	.	.	.
cord-333636-h2sg6shp	153	40	2A	2a	CD
cord-333636-h2sg6shp	153	41	)	)	-RRB-
cord-333636-h2sg6shp	153	42	.	.	.
cord-333636-h2sg6shp	154	1	Addition	addition	NN
cord-333636-h2sg6shp	154	2	of	of	IN
cord-333636-h2sg6shp	154	3	VP3	VP3	NNP
cord-333636-h2sg6shp	154	4	protein	protein	NN
cord-333636-h2sg6shp	154	5	-	-	HYPH
cord-333636-h2sg6shp	154	6	specific	specific	JJ
cord-333636-h2sg6shp	154	7	MAbs	MAbs	NNP
cord-333636-h2sg6shp	154	8	caused	cause	VBD
cord-333636-h2sg6shp	154	9	the	the	DT
cord-333636-h2sg6shp	154	10	supershift	supershift	NN
cord-333636-h2sg6shp	154	11	of	of	IN
cord-333636-h2sg6shp	154	12	all	all	PDT
cord-333636-h2sg6shp	154	13	the	the	DT
cord-333636-h2sg6shp	154	14	complexes	complex	NNS
cord-333636-h2sg6shp	154	15	(	(	-LRB-
cord-333636-h2sg6shp	154	16	Fig	Fig	NNP
cord-333636-h2sg6shp	154	17	.	.	.
cord-333636-h2sg6shp	154	18	2B	2b	CD
cord-333636-h2sg6shp	154	19	)	)	-RRB-
cord-333636-h2sg6shp	154	20	.	.	.
cord-333636-h2sg6shp	155	1	These	these	DT
cord-333636-h2sg6shp	155	2	results	result	NNS
cord-333636-h2sg6shp	155	3	unambiguously	unambiguously	RB
cord-333636-h2sg6shp	155	4	confirmed	confirm	VBD
cord-333636-h2sg6shp	155	5	that	that	IN
cord-333636-h2sg6shp	155	6	the	the	DT
cord-333636-h2sg6shp	155	7	shifted	shift	VBN
cord-333636-h2sg6shp	155	8	bands	band	NNS
cord-333636-h2sg6shp	155	9	corresponded	correspond	VBD
cord-333636-h2sg6shp	155	10	to	to	IN
cord-333636-h2sg6shp	155	11	ribonucleoprotein	ribonucleoprotein	NN
cord-333636-h2sg6shp	155	12	complexes	complex	NNS
cord-333636-h2sg6shp	155	13	containing	contain	VBG
cord-333636-h2sg6shp	155	14	VP3	VP3	NNP
cord-333636-h2sg6shp	155	15	.	.	.
cord-333636-h2sg6shp	156	1	After	after	IN
cord-333636-h2sg6shp	156	2	confirming	confirm	VBG
cord-333636-h2sg6shp	156	3	that	that	IN
cord-333636-h2sg6shp	156	4	VP3	VP3	NNP
cord-333636-h2sg6shp	156	5	bound	bind	VBN
cord-333636-h2sg6shp	156	6	ssRNA	ssrna	NN
cord-333636-h2sg6shp	156	7	probe	probe	NN
cord-333636-h2sg6shp	156	8	B	B	NNP
cord-333636-h2sg6shp	156	9	,	,	,
cord-333636-h2sg6shp	156	10	VP3-RNA	VP3-RNA	NNP
cord-333636-h2sg6shp	156	11	complex	complex	JJ
cord-333636-h2sg6shp	156	12	dissociation	dissociation	NN
cord-333636-h2sg6shp	156	13	constant	constant	NN
cord-333636-h2sg6shp	156	14	was	be	VBD
cord-333636-h2sg6shp	156	15	estimated	estimate	VBN
cord-333636-h2sg6shp	156	16	by	by	IN
cord-333636-h2sg6shp	156	17	binding	bind	VBG
cord-333636-h2sg6shp	156	18	ribonucleoprotein	ribonucleoprotein	NN
cord-333636-h2sg6shp	156	19	complexes	complex	NNS
cord-333636-h2sg6shp	156	20	to	to	IN
cord-333636-h2sg6shp	156	21	nitrocellulose	nitrocellulose	NN
cord-333636-h2sg6shp	156	22	filters	filter	NNS
cord-333636-h2sg6shp	156	23	,	,	,
cord-333636-h2sg6shp	156	24	as	as	IN
cord-333636-h2sg6shp	156	25	described	describe	VBN
cord-333636-h2sg6shp	156	26	in	in	IN
cord-333636-h2sg6shp	156	27	Materials	material	NNS
cord-333636-h2sg6shp	156	28	and	and	CC
cord-333636-h2sg6shp	156	29	Methods	method	NNS
cord-333636-h2sg6shp	156	30	.	.	.
cord-333636-h2sg6shp	157	1	The	the	DT
cord-333636-h2sg6shp	157	2	percentage	percentage	NN
cord-333636-h2sg6shp	157	3	of	of	IN
cord-333636-h2sg6shp	157	4	the	the	DT
cord-333636-h2sg6shp	157	5	retained	retain	VBN
cord-333636-h2sg6shp	157	6	radioactivity	radioactivity	NN
cord-333636-h2sg6shp	157	7	in	in	IN
cord-333636-h2sg6shp	157	8	nitrocellulose	nitrocellulose	NN
cord-333636-h2sg6shp	157	9	filters	filter	NNS
cord-333636-h2sg6shp	157	10	was	be	VBD
cord-333636-h2sg6shp	157	11	represented	represent	VBN
cord-333636-h2sg6shp	157	12	as	as	IN
cord-333636-h2sg6shp	157	13	a	a	DT
cord-333636-h2sg6shp	157	14	function	function	NN
cord-333636-h2sg6shp	157	15	of	of	IN
cord-333636-h2sg6shp	157	16	VP3	VP3	NNP
cord-333636-h2sg6shp	157	17	concentration	concentration	NN
cord-333636-h2sg6shp	157	18	(	(	-LRB-
cord-333636-h2sg6shp	157	19	Fig	Fig	NNP
cord-333636-h2sg6shp	157	20	.	.	.
cord-333636-h2sg6shp	157	21	3A	3A	NNP
cord-333636-h2sg6shp	157	22	)	)	-RRB-
cord-333636-h2sg6shp	157	23	.	.	.
cord-333636-h2sg6shp	158	1	A	a	DT
cord-333636-h2sg6shp	158	2	plateau	plateau	NN
cord-333636-h2sg6shp	158	3	of	of	IN
cord-333636-h2sg6shp	158	4	VP3-RNA	VP3-RNA	NNP
cord-333636-h2sg6shp	158	5	binding	bind	VBG
cord-333636-h2sg6shp	158	6	was	be	VBD
cord-333636-h2sg6shp	158	7	observed	observe	VBN
cord-333636-h2sg6shp	158	8	at	at	IN
cord-333636-h2sg6shp	158	9	VP3	VP3	NNP
cord-333636-h2sg6shp	158	10	concentrations	concentration	NNS
cord-333636-h2sg6shp	158	11	above	above	IN
cord-333636-h2sg6shp	158	12	10	10	CD
cord-333636-h2sg6shp	158	13	−4	−4	CD
cord-333636-h2sg6shp	159	1	mM.	mM.	NNP
cord-333636-h2sg6shp	160	1	The	the	DT
cord-333636-h2sg6shp	160	2	VP3-RNA	VP3-RNA	NNP
cord-333636-h2sg6shp	160	3	dissociation	dissociation	NN
cord-333636-h2sg6shp	160	4	constant	constant	NN
cord-333636-h2sg6shp	160	5	was	be	VBD
cord-333636-h2sg6shp	160	6	derived	derive	VBN
cord-333636-h2sg6shp	160	7	from	from	IN
cord-333636-h2sg6shp	160	8	binding	bind	VBG
cord-333636-h2sg6shp	160	9	curves	curve	NNS
cord-333636-h2sg6shp	160	10	,	,	,
cord-333636-h2sg6shp	160	11	and	and	CC
cord-333636-h2sg6shp	160	12	it	-PRON-	PRP
cord-333636-h2sg6shp	160	13	was	be	VBD
cord-333636-h2sg6shp	160	14	estimated	estimate	VBN
cord-333636-h2sg6shp	160	15	from	from	IN
cord-333636-h2sg6shp	160	16	3	3	CD
cord-333636-h2sg6shp	160	17	independent	independent	JJ
cord-333636-h2sg6shp	160	18	experiments	experiment	NNS
cord-333636-h2sg6shp	160	19	to	to	TO
cord-333636-h2sg6shp	160	20	be	be	VB
cord-333636-h2sg6shp	160	21	of	of	IN
cord-333636-h2sg6shp	160	22	2	2	CD
cord-333636-h2sg6shp	160	23	±	±	NNP
cord-333636-h2sg6shp	160	24	0.2	0.2	CD
cord-333636-h2sg6shp	160	25	×	×	CD
cord-333636-h2sg6shp	160	26	10	10	CD
cord-333636-h2sg6shp	161	1	−4	−4	NNP
cord-333636-h2sg6shp	161	2	mM	mm	NN
cord-333636-h2sg6shp	161	3	(	(	-LRB-
cord-333636-h2sg6shp	161	4	n	n	NN
cord-333636-h2sg6shp	161	5	=	=	SYM
cord-333636-h2sg6shp	161	6	3	3	CD
cord-333636-h2sg6shp	161	7	;	;	.
cord-333636-h2sg6shp	162	1	Spearman	Spearman	NNP
cord-333636-h2sg6shp	162	2	's	's	POS
cord-333636-h2sg6shp	162	3	coefficient	coefficient	NN
cord-333636-h2sg6shp	162	4	of	of	IN
cord-333636-h2sg6shp	162	5	variation	variation	NN
cord-333636-h2sg6shp	162	6	,	,	,
cord-333636-h2sg6shp	162	7	CV	cv	NN
cord-333636-h2sg6shp	162	8	=	=	SYM
cord-333636-h2sg6shp	162	9	10	10	CD
cord-333636-h2sg6shp	162	10	%	%	NN
cord-333636-h2sg6shp	162	11	)	)	-RRB-
cord-333636-h2sg6shp	162	12	.	.	.
cord-333636-h2sg6shp	163	1	Similar	similar	JJ
cord-333636-h2sg6shp	163	2	results	result	NNS
cord-333636-h2sg6shp	163	3	were	be	VBD
cord-333636-h2sg6shp	163	4	obtained	obtain	VBN
cord-333636-h2sg6shp	163	5	with	with	IN
cord-333636-h2sg6shp	163	6	different	different	JJ
cord-333636-h2sg6shp	163	7	ssRNA	ssrna	NN
cord-333636-h2sg6shp	163	8	probes	probe	NNS
cord-333636-h2sg6shp	163	9	(	(	-LRB-
cord-333636-h2sg6shp	163	10	data	datum	NNS
cord-333636-h2sg6shp	163	11	not	not	RB
cord-333636-h2sg6shp	163	12	shown	show	VBN
cord-333636-h2sg6shp	163	13	)	)	-RRB-
cord-333636-h2sg6shp	163	14	.	.	.
cord-333636-h2sg6shp	164	1	A	a	DT
cord-333636-h2sg6shp	164	2	Hill	Hill	NNP
cord-333636-h2sg6shp	164	3	plot	plot	NN
cord-333636-h2sg6shp	164	4	for	for	IN
cord-333636-h2sg6shp	164	5	the	the	DT
cord-333636-h2sg6shp	164	6	binding	binding	NN
cord-333636-h2sg6shp	164	7	of	of	IN
cord-333636-h2sg6shp	164	8	VP3	VP3	NNP
cord-333636-h2sg6shp	164	9	to	to	IN
cord-333636-h2sg6shp	164	10	ssRNA	ssRNA	VBG
cord-333636-h2sg6shp	164	11	was	be	VBD
cord-333636-h2sg6shp	164	12	derived	derive	VBN
cord-333636-h2sg6shp	164	13	(	(	-LRB-
cord-333636-h2sg6shp	164	14	Fig	Fig	NNP
cord-333636-h2sg6shp	164	15	.	.	.
cord-333636-h2sg6shp	164	16	3B	3B	NNP
cord-333636-h2sg6shp	164	17	)	)	-RRB-
cord-333636-h2sg6shp	164	18	,	,	,
cord-333636-h2sg6shp	164	19	calculating	calculate	VBG
cord-333636-h2sg6shp	164	20	a	a	DT
cord-333636-h2sg6shp	164	21	regression	regression	NN
cord-333636-h2sg6shp	164	22	line	line	NN
cord-333636-h2sg6shp	164	23	with	with	IN
cord-333636-h2sg6shp	164	24	a	a	DT
cord-333636-h2sg6shp	164	25	correlation	correlation	NN
cord-333636-h2sg6shp	164	26	coefficient	coefficient	NN
cord-333636-h2sg6shp	164	27	(	(	-LRB-
cord-333636-h2sg6shp	164	28	R	r	NN
cord-333636-h2sg6shp	164	29	=	=	SYM
cord-333636-h2sg6shp	164	30	0.9705	0.9705	CD
cord-333636-h2sg6shp	165	1	;	;	:
cord-333636-h2sg6shp	165	2	9	9	CD
cord-333636-h2sg6shp	165	3	degrees	degree	NNS
cord-333636-h2sg6shp	165	4	of	of	IN
cord-333636-h2sg6shp	165	5	freedom	freedom	NN
cord-333636-h2sg6shp	165	6	;	;	:
cord-333636-h2sg6shp	165	7	t	t	NN
cord-333636-h2sg6shp	165	8	=	=	SYM
cord-333636-h2sg6shp	165	9	12.07	12.07	CD
cord-333636-h2sg6shp	165	10	)	)	-RRB-
cord-333636-h2sg6shp	165	11	-associated	-associate	VBN
cord-333636-h2sg6shp	166	1	probability	probability	NN
cord-333636-h2sg6shp	166	2	(	(	-LRB-
cord-333636-h2sg6shp	166	3	P	p	NN
cord-333636-h2sg6shp	166	4	)	)	-RRB-
cord-333636-h2sg6shp	166	5	of	of	IN
cord-333636-h2sg6shp	166	6	9.97	9.97	CD
cord-333636-h2sg6shp	166	7	×	×	NN
cord-333636-h2sg6shp	166	8	10	10	CD
cord-333636-h2sg6shp	166	9	−5	−5	NNS
cord-333636-h2sg6shp	166	10	,	,	,
cord-333636-h2sg6shp	166	11	showing	show	VBG
cord-333636-h2sg6shp	166	12	a	a	DT
cord-333636-h2sg6shp	166	13	very	very	RB
cord-333636-h2sg6shp	166	14	significant	significant	JJ
cord-333636-h2sg6shp	166	15	correlation	correlation	NN
cord-333636-h2sg6shp	166	16	of	of	IN
cord-333636-h2sg6shp	166	17	data	datum	NNS
cord-333636-h2sg6shp	166	18	to	to	IN
cord-333636-h2sg6shp	166	19	a	a	DT
cord-333636-h2sg6shp	166	20	linear	linear	JJ
cord-333636-h2sg6shp	166	21	relationship	relationship	NN
cord-333636-h2sg6shp	166	22	.	.	.
cord-333636-h2sg6shp	167	1	The	the	DT
cord-333636-h2sg6shp	167	2	Hill	Hill	NNP
cord-333636-h2sg6shp	167	3	coefficient	coefficient	NN
cord-333636-h2sg6shp	167	4	(	(	-LRB-
cord-333636-h2sg6shp	167	5	n	n	NNP
cord-333636-h2sg6shp	167	6	H	H	NNP
cord-333636-h2sg6shp	167	7	)	)	-RRB-
cord-333636-h2sg6shp	167	8	derived	derive	VBN
cord-333636-h2sg6shp	167	9	from	from	IN
cord-333636-h2sg6shp	167	10	the	the	DT
cord-333636-h2sg6shp	167	11	regression	regression	NN
cord-333636-h2sg6shp	167	12	line	line	NN
cord-333636-h2sg6shp	167	13	was	be	VBD
cord-333636-h2sg6shp	167	14	0.97	0.97	CD
cord-333636-h2sg6shp	167	15	(	(	-LRB-
cord-333636-h2sg6shp	167	16	Fig	Fig	NNP
cord-333636-h2sg6shp	167	17	.	.	.
cord-333636-h2sg6shp	167	18	3B	3B	NNP
cord-333636-h2sg6shp	167	19	)	)	-RRB-
cord-333636-h2sg6shp	167	20	,	,	,
cord-333636-h2sg6shp	167	21	slightly	slightly	RB
cord-333636-h2sg6shp	167	22	lower	low	JJR
cord-333636-h2sg6shp	167	23	than	than	IN
cord-333636-h2sg6shp	167	24	1	1	CD
cord-333636-h2sg6shp	167	25	,	,	,
cord-333636-h2sg6shp	167	26	showing	show	VBG
cord-333636-h2sg6shp	167	27	that	that	IN
cord-333636-h2sg6shp	167	28	VP3	VP3	NNP
cord-333636-h2sg6shp	167	29	bound	bind	VBN
cord-333636-h2sg6shp	167	30	ssRNA	ssrna	RB
cord-333636-h2sg6shp	167	31	in	in	IN
cord-333636-h2sg6shp	167	32	a	a	DT
cord-333636-h2sg6shp	167	33	non	non	JJ
cord-333636-h2sg6shp	167	34	-	-	JJ
cord-333636-h2sg6shp	167	35	cooperative	cooperative	JJ
cord-333636-h2sg6shp	167	36	manner	manner	NN
cord-333636-h2sg6shp	167	37	.	.	.
cord-333636-h2sg6shp	168	1	The	the	DT
cord-333636-h2sg6shp	168	2	K	K	NNP
cord-333636-h2sg6shp	168	3	0.5	0.5	CD
cord-333636-h2sg6shp	168	4	was	be	VBD
cord-333636-h2sg6shp	168	5	calculated	calculate	VBN
cord-333636-h2sg6shp	168	6	from	from	IN
cord-333636-h2sg6shp	168	7	the	the	DT
cord-333636-h2sg6shp	168	8	regression	regression	NN
cord-333636-h2sg6shp	168	9	line	line	NN
cord-333636-h2sg6shp	168	10	,	,	,
cord-333636-h2sg6shp	168	11	giving	give	VBG
cord-333636-h2sg6shp	168	12	a	a	DT
cord-333636-h2sg6shp	168	13	result	result	NN
cord-333636-h2sg6shp	168	14	of	of	IN
cord-333636-h2sg6shp	168	15	1.02	1.02	CD
cord-333636-h2sg6shp	168	16	×	×	NN
cord-333636-h2sg6shp	168	17	10	10	CD
cord-333636-h2sg6shp	168	18	−4	−4	NNP
cord-333636-h2sg6shp	168	19	mM	mm	CD
cord-333636-h2sg6shp	168	20	,	,	,
cord-333636-h2sg6shp	168	21	close	close	JJ
cord-333636-h2sg6shp	168	22	to	to	IN
cord-333636-h2sg6shp	168	23	the	the	DT
cord-333636-h2sg6shp	168	24	estimated	estimate	VBN
cord-333636-h2sg6shp	168	25	apparent	apparent	JJ
cord-333636-h2sg6shp	168	26	Kd	Kd	NNP
cord-333636-h2sg6shp	168	27	(	(	-LRB-
cord-333636-h2sg6shp	168	28	Fig	Fig	NNP
cord-333636-h2sg6shp	168	29	.	.	.
cord-333636-h2sg6shp	168	30	3B	3B	NNP
cord-333636-h2sg6shp	168	31	)	)	-RRB-
cord-333636-h2sg6shp	168	32	.	.	.
cord-333636-h2sg6shp	168	33	 	 	_SP
cord-333636-h2sg6shp	169	1	The	the	DT
cord-333636-h2sg6shp	169	2	experiments	experiment	NNS
cord-333636-h2sg6shp	169	3	described	describe	VBN
cord-333636-h2sg6shp	169	4	above	above	RB
cord-333636-h2sg6shp	169	5	were	be	VBD
cord-333636-h2sg6shp	169	6	performed	perform	VBN
cord-333636-h2sg6shp	169	7	using	use	VBG
cord-333636-h2sg6shp	169	8	probe	probe	NN
cord-333636-h2sg6shp	169	9	B	B	NNP
cord-333636-h2sg6shp	169	10	that	that	WDT
cord-333636-h2sg6shp	169	11	contained	contain	VBD
cord-333636-h2sg6shp	169	12	the	the	DT
cord-333636-h2sg6shp	169	13	5	5	CD
cord-333636-h2sg6shp	169	14	and	and	CC
cord-333636-h2sg6shp	169	15	3	3	CD
cord-333636-h2sg6shp	169	16	UTRs	utr	NNS
cord-333636-h2sg6shp	169	17	from	from	IN
cord-333636-h2sg6shp	169	18	IBDV	IBDV	NNP
cord-333636-h2sg6shp	169	19	genome	genome	NN
cord-333636-h2sg6shp	169	20	segment	segment	NN
cord-333636-h2sg6shp	169	21	B.	B.	NNP
cord-333636-h2sg6shp	169	22	Competition	Competition	NNP
cord-333636-h2sg6shp	169	23	experiments	experiment	NNS
cord-333636-h2sg6shp	169	24	were	be	VBD
cord-333636-h2sg6shp	169	25	performed	perform	VBN
cord-333636-h2sg6shp	169	26	to	to	TO
cord-333636-h2sg6shp	169	27	find	find	VB
cord-333636-h2sg6shp	169	28	out	out	RP
cord-333636-h2sg6shp	169	29	whether	whether	IN
cord-333636-h2sg6shp	169	30	the	the	DT
cord-333636-h2sg6shp	169	31	VP3-RNA	VP3-RNA	NNP
cord-333636-h2sg6shp	169	32	interaction	interaction	NN
cord-333636-h2sg6shp	169	33	was	be	VBD
cord-333636-h2sg6shp	169	34	sequence	sequence	NN
cord-333636-h2sg6shp	169	35	-	-	HYPH
cord-333636-h2sg6shp	169	36	specific	specific	JJ
cord-333636-h2sg6shp	169	37	.	.	.
cord-333636-h2sg6shp	170	1	For	for	IN
cord-333636-h2sg6shp	170	2	this	this	DT
cord-333636-h2sg6shp	170	3	,	,	,
cord-333636-h2sg6shp	170	4	increasing	increase	VBG
cord-333636-h2sg6shp	170	5	concentrations	concentration	NNS
cord-333636-h2sg6shp	170	6	of	of	IN
cord-333636-h2sg6shp	170	7	different	different	JJ
cord-333636-h2sg6shp	170	8	unlabeled	unlabeled	JJ
cord-333636-h2sg6shp	170	9	RNA	RNA	NNP
cord-333636-h2sg6shp	170	10	or	or	CC
cord-333636-h2sg6shp	170	11	DNA	dna	NN
cord-333636-h2sg6shp	170	12	competitor	competitor	NN
cord-333636-h2sg6shp	170	13	probes	probe	NNS
cord-333636-h2sg6shp	170	14	were	be	VBD
cord-333636-h2sg6shp	170	15	included	include	VBN
cord-333636-h2sg6shp	170	16	in	in	IN
cord-333636-h2sg6shp	170	17	the	the	DT
cord-333636-h2sg6shp	170	18	VP3	VP3	NNP
cord-333636-h2sg6shp	170	19	/	/	SYM
cord-333636-h2sg6shp	170	20	RNA	RNA	NNP
cord-333636-h2sg6shp	170	21	reaction	reaction	NN
cord-333636-h2sg6shp	170	22	mixtures	mixture	NNS
cord-333636-h2sg6shp	170	23	.	.	.
cord-333636-h2sg6shp	171	1	After	after	IN
cord-333636-h2sg6shp	171	2	incubation	incubation	NN
cord-333636-h2sg6shp	171	3	,	,	,
cord-333636-h2sg6shp	171	4	samples	sample	NNS
cord-333636-h2sg6shp	171	5	were	be	VBD
cord-333636-h2sg6shp	171	6	filtered	filter	VBN
cord-333636-h2sg6shp	171	7	though	though	IN
cord-333636-h2sg6shp	171	8	nitrocellulose	nitrocellulose	JJ
cord-333636-h2sg6shp	171	9	membranes	membrane	NNS
cord-333636-h2sg6shp	171	10	,	,	,
cord-333636-h2sg6shp	171	11	and	and	CC
cord-333636-h2sg6shp	171	12	the	the	DT
cord-333636-h2sg6shp	171	13	amount	amount	NN
cord-333636-h2sg6shp	171	14	of	of	IN
cord-333636-h2sg6shp	171	15	retained	retain	VBN
cord-333636-h2sg6shp	171	16	radioactivity	radioactivity	NN
cord-333636-h2sg6shp	171	17	estimated	estimate	VBN
cord-333636-h2sg6shp	171	18	.	.	.
cord-333636-h2sg6shp	172	1	As	as	IN
cord-333636-h2sg6shp	172	2	expected	expect	VBN
cord-333636-h2sg6shp	172	3	,	,	,
cord-333636-h2sg6shp	172	4	binding	bind	VBG
cord-333636-h2sg6shp	172	5	of	of	IN
cord-333636-h2sg6shp	172	6	VP3	VP3	NNP
cord-333636-h2sg6shp	172	7	to	to	IN
cord-333636-h2sg6shp	172	8	labeled	label	VBN
cord-333636-h2sg6shp	172	9	ssRNA	ssRNA	.
cord-333636-h2sg6shp	173	1	probe	probe	NNP
cord-333636-h2sg6shp	173	2	B	B	NNP
cord-333636-h2sg6shp	173	3	was	be	VBD
cord-333636-h2sg6shp	173	4	efficiently	efficiently	RB
cord-333636-h2sg6shp	173	5	competed	compete	VBN
cord-333636-h2sg6shp	173	6	by	by	IN
cord-333636-h2sg6shp	173	7	the	the	DT
cord-333636-h2sg6shp	173	8	addition	addition	NN
cord-333636-h2sg6shp	173	9	of	of	IN
cord-333636-h2sg6shp	173	10	unlabeled	unlabeled	JJ
cord-333636-h2sg6shp	173	11	probe	probe	NN
cord-333636-h2sg6shp	173	12	B	b	NN
cord-333636-h2sg6shp	173	13	(	(	-LRB-
cord-333636-h2sg6shp	173	14	Fig	Fig	NNP
cord-333636-h2sg6shp	173	15	.	.	.
cord-333636-h2sg6shp	173	16	4A	4a	CD
cord-333636-h2sg6shp	173	17	)	)	-RRB-
cord-333636-h2sg6shp	173	18	.	.	.
cord-333636-h2sg6shp	174	1	A	a	DT
cord-333636-h2sg6shp	174	2	similar	similar	JJ
cord-333636-h2sg6shp	174	3	result	result	NN
cord-333636-h2sg6shp	174	4	was	be	VBD
cord-333636-h2sg6shp	174	5	obtained	obtain	VBN
cord-333636-h2sg6shp	174	6	when	when	WRB
cord-333636-h2sg6shp	174	7	ssRNA	ssRNA	``
cord-333636-h2sg6shp	174	8	probe	probe	NN
cord-333636-h2sg6shp	174	9	A	A	NNP
cord-333636-h2sg6shp	174	10	was	be	VBD
cord-333636-h2sg6shp	174	11	used	use	VBN
cord-333636-h2sg6shp	174	12	(	(	-LRB-
cord-333636-h2sg6shp	174	13	Fig	Fig	NNP
cord-333636-h2sg6shp	174	14	.	.	.
cord-333636-h2sg6shp	174	15	4B	4b	CD
cord-333636-h2sg6shp	174	16	)	)	-RRB-
cord-333636-h2sg6shp	174	17	.	.	.
cord-333636-h2sg6shp	175	1	Nonviral	Nonviral	NNP
cord-333636-h2sg6shp	175	2	ssRNA	ssRNA	NNP
cord-333636-h2sg6shp	176	1	Fig	Fig	NNP
cord-333636-h2sg6shp	176	2	.	.	.
cord-333636-h2sg6shp	177	1	4	4	LS
cord-333636-h2sg6shp	177	2	.	.	.
cord-333636-h2sg6shp	178	1	Inhibition	inhibition	NN
cord-333636-h2sg6shp	178	2	of	of	IN
cord-333636-h2sg6shp	178	3	VP3	VP3	NNP
cord-333636-h2sg6shp	178	4	binding	bind	VBG
cord-333636-h2sg6shp	178	5	to	to	IN
cord-333636-h2sg6shp	178	6	labeled	label	VBN
cord-333636-h2sg6shp	178	7	ssRNA	ssrna	NN
cord-333636-h2sg6shp	178	8	probes	probe	NNS
cord-333636-h2sg6shp	178	9	by	by	IN
cord-333636-h2sg6shp	178	10	unlabeled	unlabeled	JJ
cord-333636-h2sg6shp	178	11	competitor	competitor	NN
cord-333636-h2sg6shp	178	12	RNAs	rna	NNS
cord-333636-h2sg6shp	178	13	.	.	.
cord-333636-h2sg6shp	179	1	A	a	DT
cord-333636-h2sg6shp	179	2	Graphs	graph	NNS
cord-333636-h2sg6shp	179	3	represent	represent	VBP
cord-333636-h2sg6shp	179	4	the	the	DT
cord-333636-h2sg6shp	179	5	percentage	percentage	NN
cord-333636-h2sg6shp	179	6	of	of	IN
cord-333636-h2sg6shp	179	7	retained	retain	VBN
cord-333636-h2sg6shp	179	8	radiactivity	radiactivity	NN
cord-333636-h2sg6shp	179	9	in	in	IN
cord-333636-h2sg6shp	179	10	nitrocellulose	nitrocellulose	JJ
cord-333636-h2sg6shp	179	11	membranes	membrane	NNS
cord-333636-h2sg6shp	179	12	from	from	IN
cord-333636-h2sg6shp	179	13	complexes	complex	NNS
cord-333636-h2sg6shp	179	14	formed	form	VBN
cord-333636-h2sg6shp	179	15	by	by	IN
cord-333636-h2sg6shp	179	16	VP3	VP3	NNP
cord-333636-h2sg6shp	179	17	and	and	CC
cord-333636-h2sg6shp	179	18	labeled	label	VBD
cord-333636-h2sg6shp	179	19	ssRNA	ssrna	NN
cord-333636-h2sg6shp	179	20	probe	probe	NN
cord-333636-h2sg6shp	179	21	B	B	NNP
cord-333636-h2sg6shp	179	22	,	,	,
cord-333636-h2sg6shp	179	23	as	as	IN
cord-333636-h2sg6shp	179	24	a	a	DT
cord-333636-h2sg6shp	179	25	function	function	NN
cord-333636-h2sg6shp	179	26	of	of	IN
cord-333636-h2sg6shp	179	27	the	the	DT
cord-333636-h2sg6shp	179	28	ratios	ratio	NNS
cord-333636-h2sg6shp	179	29	of	of	IN
cord-333636-h2sg6shp	179	30	unlabeled	unlabeled	JJ
cord-333636-h2sg6shp	179	31	ssRNA	ssrna	NN
cord-333636-h2sg6shp	179	32	probes	probe	NNS
cord-333636-h2sg6shp	179	33	B	b	NN
cord-333636-h2sg6shp	179	34	(	(	-LRB-
cord-333636-h2sg6shp	179	35	left	left	JJ
cord-333636-h2sg6shp	179	36	graph	graph	NN
cord-333636-h2sg6shp	179	37	)	)	-RRB-
cord-333636-h2sg6shp	179	38	or	or	CC
cord-333636-h2sg6shp	179	39	PL	PL	NNP
cord-333636-h2sg6shp	179	40	(	(	-LRB-
cord-333636-h2sg6shp	179	41	right	right	JJ
cord-333636-h2sg6shp	179	42	graph	graph	NN
cord-333636-h2sg6shp	179	43	)	)	-RRB-
cord-333636-h2sg6shp	179	44	to	to	IN
cord-333636-h2sg6shp	179	45	labeled	label	VBN
cord-333636-h2sg6shp	179	46	probe	probe	NN
cord-333636-h2sg6shp	179	47	B.	B.	NNP
cord-333636-h2sg6shp	179	48	Data	Data	NNP
cord-333636-h2sg6shp	179	49	is	be	VBZ
cord-333636-h2sg6shp	179	50	represented	represent	VBN
cord-333636-h2sg6shp	179	51	as	as	IN
cord-333636-h2sg6shp	179	52	the	the	DT
cord-333636-h2sg6shp	179	53	mean	mean	NN
cord-333636-h2sg6shp	179	54	of	of	IN
cord-333636-h2sg6shp	179	55	three	three	CD
cord-333636-h2sg6shp	179	56	independent	independent	JJ
cord-333636-h2sg6shp	179	57	experiments	experiment	NNS
cord-333636-h2sg6shp	179	58	together	together	RB
cord-333636-h2sg6shp	179	59	with	with	IN
cord-333636-h2sg6shp	179	60	error	error	NN
cord-333636-h2sg6shp	179	61	bars	bar	NNS
cord-333636-h2sg6shp	179	62	.	.	.
cord-333636-h2sg6shp	180	1	B	B	NNP
cord-333636-h2sg6shp	180	2	Graphs	graph	NNS
cord-333636-h2sg6shp	180	3	represent	represent	VBP
cord-333636-h2sg6shp	180	4	the	the	DT
cord-333636-h2sg6shp	180	5	percentage	percentage	NN
cord-333636-h2sg6shp	180	6	of	of	IN
cord-333636-h2sg6shp	180	7	retained	retain	VBN
cord-333636-h2sg6shp	180	8	radiactivity	radiactivity	NN
cord-333636-h2sg6shp	180	9	in	in	IN
cord-333636-h2sg6shp	180	10	nitrocellulose	nitrocellulose	JJ
cord-333636-h2sg6shp	180	11	membranes	membrane	NNS
cord-333636-h2sg6shp	180	12	from	from	IN
cord-333636-h2sg6shp	180	13	complexes	complex	NNS
cord-333636-h2sg6shp	180	14	formed	form	VBN
cord-333636-h2sg6shp	180	15	by	by	IN
cord-333636-h2sg6shp	180	16	VP3	VP3	NNP
cord-333636-h2sg6shp	180	17	and	and	CC
cord-333636-h2sg6shp	180	18	labeled	label	VBD
cord-333636-h2sg6shp	180	19	ssRNA	ssRNA	``
cord-333636-h2sg6shp	180	20	probe	probe	NN
cord-333636-h2sg6shp	180	21	A	A	NNP
cord-333636-h2sg6shp	180	22	,	,	,
cord-333636-h2sg6shp	180	23	as	as	IN
cord-333636-h2sg6shp	180	24	a	a	DT
cord-333636-h2sg6shp	180	25	function	function	NN
cord-333636-h2sg6shp	180	26	of	of	IN
cord-333636-h2sg6shp	180	27	the	the	DT
cord-333636-h2sg6shp	180	28	ratio	ratio	NN
cord-333636-h2sg6shp	180	29	of	of	IN
cord-333636-h2sg6shp	180	30	unlabeled	unlabeled	JJ
cord-333636-h2sg6shp	180	31	ssRNA	ssrna	NN
cord-333636-h2sg6shp	180	32	probes	probe	NNS
cord-333636-h2sg6shp	180	33	A	a	DT
cord-333636-h2sg6shp	180	34	(	(	-LRB-
cord-333636-h2sg6shp	180	35	left	left	JJ
cord-333636-h2sg6shp	180	36	graph	graph	NN
cord-333636-h2sg6shp	180	37	)	)	-RRB-
cord-333636-h2sg6shp	180	38	or	or	CC
cord-333636-h2sg6shp	180	39	GFP	GFP	NNP
cord-333636-h2sg6shp	180	40	(	(	-LRB-
cord-333636-h2sg6shp	180	41	right	right	JJ
cord-333636-h2sg6shp	180	42	graph	graph	NN
cord-333636-h2sg6shp	180	43	)	)	-RRB-
cord-333636-h2sg6shp	180	44	to	to	IN
cord-333636-h2sg6shp	180	45	labeled	label	VBN
cord-333636-h2sg6shp	180	46	probe	probe	NN
cord-333636-h2sg6shp	180	47	A	a	DT
cord-333636-h2sg6shp	180	48	probes	probe	NNS
cord-333636-h2sg6shp	180	49	PL	pl	NN
cord-333636-h2sg6shp	180	50	and	and	CC
cord-333636-h2sg6shp	180	51	GFP	GFP	NNP
cord-333636-h2sg6shp	180	52	also	also	RB
cord-333636-h2sg6shp	180	53	competed	compete	VBD
cord-333636-h2sg6shp	180	54	with	with	IN
cord-333636-h2sg6shp	180	55	probes	probe	NNS
cord-333636-h2sg6shp	180	56	B	b	NN
cord-333636-h2sg6shp	180	57	and	and	CC
cord-333636-h2sg6shp	180	58	A	A	NNP
cord-333636-h2sg6shp	180	59	,	,	,
cord-333636-h2sg6shp	180	60	respectively	respectively	RB
cord-333636-h2sg6shp	180	61	(	(	-LRB-
cord-333636-h2sg6shp	180	62	Fig	Fig	NNP
cord-333636-h2sg6shp	180	63	.	.	.
cord-333636-h2sg6shp	180	64	4A	4a	CD
cord-333636-h2sg6shp	180	65	and	and	CC
cord-333636-h2sg6shp	180	66	B	B	NNP
cord-333636-h2sg6shp	180	67	)	)	-RRB-
cord-333636-h2sg6shp	180	68	,	,	,
cord-333636-h2sg6shp	180	69	indicating	indicate	VBG
cord-333636-h2sg6shp	180	70	that	that	IN
cord-333636-h2sg6shp	180	71	VP3	VP3	NNP
cord-333636-h2sg6shp	180	72	binding	bind	VBG
cord-333636-h2sg6shp	180	73	to	to	IN
cord-333636-h2sg6shp	180	74	ssRNA	ssRNA	VBG
cord-333636-h2sg6shp	180	75	was	be	VBD
cord-333636-h2sg6shp	180	76	sequence	sequence	NN
cord-333636-h2sg6shp	180	77	-	-	HYPH
cord-333636-h2sg6shp	180	78	independent	independent	JJ
cord-333636-h2sg6shp	180	79	.	.	.
cord-333636-h2sg6shp	181	1	IBDV	IBDV	NNP
cord-333636-h2sg6shp	181	2	contains	contain	VBZ
cord-333636-h2sg6shp	181	3	a	a	DT
cord-333636-h2sg6shp	181	4	dsRNA	dsrna	NN
cord-333636-h2sg6shp	181	5	genome	genome	NN
cord-333636-h2sg6shp	181	6	probably	probably	RB
cord-333636-h2sg6shp	181	7	in	in	IN
cord-333636-h2sg6shp	181	8	close	close	JJ
cord-333636-h2sg6shp	181	9	contact	contact	NN
cord-333636-h2sg6shp	181	10	with	with	IN
cord-333636-h2sg6shp	181	11	VP3	vp3	JJ
cord-333636-h2sg6shp	181	12	molecules	molecule	NNS
cord-333636-h2sg6shp	181	13	in	in	IN
cord-333636-h2sg6shp	181	14	IBDV	IBDV	NNP
cord-333636-h2sg6shp	181	15	virions	virion	NNS
cord-333636-h2sg6shp	181	16	[	[	-LRB-
cord-333636-h2sg6shp	181	17	6	6	CD
cord-333636-h2sg6shp	181	18	,	,	,
cord-333636-h2sg6shp	181	19	9	9	CD
cord-333636-h2sg6shp	181	20	]	]	-RRB-
cord-333636-h2sg6shp	181	21	.	.	.
cord-333636-h2sg6shp	182	1	To	to	TO
cord-333636-h2sg6shp	182	2	find	find	VB
cord-333636-h2sg6shp	182	3	out	out	RP
cord-333636-h2sg6shp	182	4	whether	whether	IN
cord-333636-h2sg6shp	182	5	VP3	VP3	NNP
cord-333636-h2sg6shp	182	6	presented	present	VBD
cord-333636-h2sg6shp	182	7	a	a	DT
cord-333636-h2sg6shp	182	8	binding	binding	JJ
cord-333636-h2sg6shp	182	9	activity	activity	NN
cord-333636-h2sg6shp	182	10	to	to	IN
cord-333636-h2sg6shp	182	11	IBDV	IBDV	NNP
cord-333636-h2sg6shp	182	12	dsRNA	dsrna	NN
cord-333636-h2sg6shp	182	13	,	,	,
cord-333636-h2sg6shp	182	14	competition	competition	NN
cord-333636-h2sg6shp	182	15	experiments	experiment	NNS
cord-333636-h2sg6shp	182	16	using	use	VBG
cord-333636-h2sg6shp	182	17	purified	purify	VBN
cord-333636-h2sg6shp	182	18	IBDV	IBDV	NNP
cord-333636-h2sg6shp	182	19	dsRNA	dsRNA	NNS
cord-333636-h2sg6shp	182	20	were	be	VBD
cord-333636-h2sg6shp	182	21	performed	perform	VBN
cord-333636-h2sg6shp	182	22	.	.	.
cord-333636-h2sg6shp	183	1	As	as	IN
cord-333636-h2sg6shp	183	2	shown	show	VBN
cord-333636-h2sg6shp	183	3	in	in	IN
cord-333636-h2sg6shp	183	4	Fig	Fig	NNP
cord-333636-h2sg6shp	183	5	.	.	.
cord-333636-h2sg6shp	184	1	5	5	LS
cord-333636-h2sg6shp	184	2	,	,	,
cord-333636-h2sg6shp	184	3	IBDV	IBDV	NNP
cord-333636-h2sg6shp	184	4	purified	purify	VBN
cord-333636-h2sg6shp	184	5	dsRNA	dsrna	NN
cord-333636-h2sg6shp	184	6	segment	segment	NN
cord-333636-h2sg6shp	184	7	A	a	DT
cord-333636-h2sg6shp	184	8	efficiently	efficiently	RB
cord-333636-h2sg6shp	184	9	competed	compete	VBD
cord-333636-h2sg6shp	184	10	the	the	DT
cord-333636-h2sg6shp	184	11	binding	binding	NN
cord-333636-h2sg6shp	184	12	of	of	IN
cord-333636-h2sg6shp	184	13	VP3	VP3	NNP
cord-333636-h2sg6shp	184	14	to	to	IN
cord-333636-h2sg6shp	184	15	labeled	label	VBN
cord-333636-h2sg6shp	184	16	ssRNA	ssRNA	``
cord-333636-h2sg6shp	184	17	probe	probe	NN
cord-333636-h2sg6shp	184	18	A.	a.	NN
cord-333636-h2sg6shp	185	1	This	this	DT
cord-333636-h2sg6shp	185	2	also	also	RB
cord-333636-h2sg6shp	185	3	suggested	suggest	VBD
cord-333636-h2sg6shp	185	4	that	that	IN
cord-333636-h2sg6shp	185	5	VP3	VP3	NNP
cord-333636-h2sg6shp	185	6	probably	probably	RB
cord-333636-h2sg6shp	185	7	bound	bind	VBD
cord-333636-h2sg6shp	185	8	ssRNA	ssrna	NN
cord-333636-h2sg6shp	185	9	and	and	CC
cord-333636-h2sg6shp	185	10	dsRNA	dsRNA	NNS
cord-333636-h2sg6shp	185	11	through	through	IN
cord-333636-h2sg6shp	185	12	the	the	DT
cord-333636-h2sg6shp	185	13	same	same	JJ
cord-333636-h2sg6shp	185	14	interaction	interaction	NN
cord-333636-h2sg6shp	185	15	domain	domain	NN
cord-333636-h2sg6shp	185	16	.	.	.
cord-333636-h2sg6shp	186	1	Interestingly	interestingly	RB
cord-333636-h2sg6shp	186	2	,	,	,
cord-333636-h2sg6shp	186	3	dsDNA	dsdna	RB
cord-333636-h2sg6shp	186	4	did	do	VBD
cord-333636-h2sg6shp	186	5	not	not	RB
cord-333636-h2sg6shp	186	6	significantly	significantly	RB
cord-333636-h2sg6shp	186	7	compete	compete	VB
cord-333636-h2sg6shp	186	8	the	the	DT
cord-333636-h2sg6shp	186	9	binding	binding	NN
cord-333636-h2sg6shp	186	10	(	(	-LRB-
cord-333636-h2sg6shp	186	11	Fig	Fig	NNP
cord-333636-h2sg6shp	186	12	.	.	.
cord-333636-h2sg6shp	186	13	5	5	CD
cord-333636-h2sg6shp	186	14	)	)	-RRB-
cord-333636-h2sg6shp	186	15	.	.	.
cord-333636-h2sg6shp	187	1	Single	single	JJ
cord-333636-h2sg6shp	187	2	-	-	HYPH
cord-333636-h2sg6shp	187	3	stranded	strand	VBN
cord-333636-h2sg6shp	187	4	DNA	dna	NN
cord-333636-h2sg6shp	187	5	efficiently	efficiently	RB
cord-333636-h2sg6shp	187	6	competed	compete	VBD
cord-333636-h2sg6shp	187	7	with	with	IN
cord-333636-h2sg6shp	187	8	the	the	DT
cord-333636-h2sg6shp	187	9	binding	binding	NN
cord-333636-h2sg6shp	187	10	of	of	IN
cord-333636-h2sg6shp	187	11	VP3	VP3	NNP
cord-333636-h2sg6shp	187	12	to	to	TO
cord-333636-h2sg6shp	187	13	probe	probe	VB
cord-333636-h2sg6shp	187	14	A	a	DT
cord-333636-h2sg6shp	187	15	,	,	,
cord-333636-h2sg6shp	187	16	showing	show	VBG
cord-333636-h2sg6shp	187	17	that	that	IN
cord-333636-h2sg6shp	187	18	VP3	VP3	NNP
cord-333636-h2sg6shp	187	19	also	also	RB
cord-333636-h2sg6shp	187	20	bound	bind	VBD
cord-333636-h2sg6shp	187	21	ssDNA	ssdna	RB
cord-333636-h2sg6shp	188	1	(	(	-LRB-
cord-333636-h2sg6shp	188	2	Fig	Fig	NNP
cord-333636-h2sg6shp	188	3	.	.	NNP
cord-333636-h2sg6shp	188	4	5	5	CD
cord-333636-h2sg6shp	188	5	)	)	-RRB-
cord-333636-h2sg6shp	188	6	.	.	.
cord-333636-h2sg6shp	189	1	In	in	IN
cord-333636-h2sg6shp	189	2	order	order	NN
cord-333636-h2sg6shp	189	3	to	to	TO
cord-333636-h2sg6shp	189	4	identify	identify	VB
cord-333636-h2sg6shp	189	5	the	the	DT
cord-333636-h2sg6shp	189	6	VP3	VP3	NNP
cord-333636-h2sg6shp	189	7	protein	protein	NN
cord-333636-h2sg6shp	189	8	region	region	NN
cord-333636-h2sg6shp	189	9	involved	involve	VBN
cord-333636-h2sg6shp	189	10	in	in	IN
cord-333636-h2sg6shp	189	11	ssRNA	ssrna	RB
cord-333636-h2sg6shp	189	12	-	-	HYPH
cord-333636-h2sg6shp	189	13	binding	bind	VBG
cord-333636-h2sg6shp	189	14	,	,	,
cord-333636-h2sg6shp	189	15	a	a	DT
cord-333636-h2sg6shp	189	16	set	set	NN
cord-333636-h2sg6shp	189	17	of	of	IN
cord-333636-h2sg6shp	189	18	mutants	mutant	NNS
cord-333636-h2sg6shp	189	19	with	with	IN
cord-333636-h2sg6shp	189	20	deletions	deletion	NNS
cord-333636-h2sg6shp	189	21	of	of	IN
cord-333636-h2sg6shp	189	22	about	about	RB
cord-333636-h2sg6shp	189	23	20	20	CD
cord-333636-h2sg6shp	189	24	aa	aa	NN
cord-333636-h2sg6shp	189	25	covering	cover	VBG
cord-333636-h2sg6shp	189	26	the	the	DT
cord-333636-h2sg6shp	189	27	whole	whole	JJ
cord-333636-h2sg6shp	189	28	protein	protein	NN
cord-333636-h2sg6shp	189	29	was	be	VBD
cord-333636-h2sg6shp	189	30	generated	generate	VBN
cord-333636-h2sg6shp	189	31	(	(	-LRB-
cord-333636-h2sg6shp	189	32	Fig	Fig	NNP
cord-333636-h2sg6shp	189	33	.	.	.
cord-333636-h2sg6shp	189	34	6A	6a	CD
cord-333636-h2sg6shp	189	35	)	)	-RRB-
cord-333636-h2sg6shp	189	36	.	.	.
cord-333636-h2sg6shp	190	1	VP3	VP3	NNP
cord-333636-h2sg6shp	190	2	deletion	deletion	NN
cord-333636-h2sg6shp	190	3	mutants	mutant	NNS
cord-333636-h2sg6shp	190	4	were	be	VBD
cord-333636-h2sg6shp	190	5	expressed	express	VBN
cord-333636-h2sg6shp	190	6	and	and	CC
cord-333636-h2sg6shp	190	7	purified	purify	VBN
cord-333636-h2sg6shp	190	8	as	as	IN
cord-333636-h2sg6shp	190	9	described	describe	VBN
cord-333636-h2sg6shp	190	10	in	in	IN
cord-333636-h2sg6shp	190	11	Materials	material	NNS
cord-333636-h2sg6shp	190	12	and	and	CC
cord-333636-h2sg6shp	190	13	Methods	method	NNS
cord-333636-h2sg6shp	190	14	.	.	.
cord-333636-h2sg6shp	191	1	After	after	IN
cord-333636-h2sg6shp	191	2	purification	purification	NN
cord-333636-h2sg6shp	191	3	,	,	,
cord-333636-h2sg6shp	191	4	proteins	protein	NNS
cord-333636-h2sg6shp	191	5	were	be	VBD
cord-333636-h2sg6shp	191	6	analyzed	analyze	VBN
cord-333636-h2sg6shp	191	7	and	and	CC
cord-333636-h2sg6shp	191	8	quantified	quantify	VBN
cord-333636-h2sg6shp	191	9	by	by	IN
cord-333636-h2sg6shp	191	10	SDS	SDS	NNP
cord-333636-h2sg6shp	191	11	-	-	HYPH
cord-333636-h2sg6shp	191	12	PAGE	PAGE	NNP
cord-333636-h2sg6shp	191	13	and	and	CC
cord-333636-h2sg6shp	191	14	Coomassie	Coomassie	NNP
cord-333636-h2sg6shp	191	15	staining	staining	NN
cord-333636-h2sg6shp	191	16	(	(	-LRB-
cord-333636-h2sg6shp	191	17	Fig	Fig	NNP
cord-333636-h2sg6shp	191	18	.	.	.
cord-333636-h2sg6shp	191	19	6B	6b	CD
cord-333636-h2sg6shp	191	20	)	)	-RRB-
cord-333636-h2sg6shp	191	21	.	.	.
cord-333636-h2sg6shp	192	1	Interestingly	interestingly	RB
cord-333636-h2sg6shp	192	2	,	,	,
cord-333636-h2sg6shp	192	3	except	except	IN
cord-333636-h2sg6shp	192	4	for	for	IN
cord-333636-h2sg6shp	192	5	the	the	DT
cord-333636-h2sg6shp	192	6	VP3	VP3	NNP
cord-333636-h2sg6shp	192	7	971	971	CD
cord-333636-h2sg6shp	192	8	-	-	HYPH
cord-333636-h2sg6shp	192	9	1012	1012	CD
cord-333636-h2sg6shp	192	10	deletion	deletion	NN
cord-333636-h2sg6shp	192	11	mutant	mutant	NN
cord-333636-h2sg6shp	192	12	,	,	,
cord-333636-h2sg6shp	192	13	the	the	DT
cord-333636-h2sg6shp	192	14	samples	sample	NNS
cord-333636-h2sg6shp	192	15	contained	contain	VBD
cord-333636-h2sg6shp	192	16	two	two	CD
cord-333636-h2sg6shp	192	17	VP3	VP3	NNP
cord-333636-h2sg6shp	192	18	electrophoretic	electrophoretic	JJ
cord-333636-h2sg6shp	192	19	forms	form	NNS
cord-333636-h2sg6shp	192	20	,	,	,
cord-333636-h2sg6shp	192	21	resulting	result	VBG
cord-333636-h2sg6shp	192	22	in	in	IN
cord-333636-h2sg6shp	192	23	doublets	doublet	NNS
cord-333636-h2sg6shp	192	24	in	in	IN
cord-333636-h2sg6shp	192	25	SDS	SDS	NNP
cord-333636-h2sg6shp	192	26	-	-	HYPH
cord-333636-h2sg6shp	192	27	PAGE	page	NN
cord-333636-h2sg6shp	192	28	gels	gel	NNS
cord-333636-h2sg6shp	192	29	(	(	-LRB-
cord-333636-h2sg6shp	192	30	Fig	Fig	NNP
cord-333636-h2sg6shp	192	31	.	.	.
cord-333636-h2sg6shp	192	32	6B	6b	CD
cord-333636-h2sg6shp	192	33	)	)	-RRB-
cord-333636-h2sg6shp	192	34	.	.	.
cord-333636-h2sg6shp	193	1	Bands	band	NNS
cord-333636-h2sg6shp	193	2	corresponding	correspond	VBG
cord-333636-h2sg6shp	193	3	to	to	IN
cord-333636-h2sg6shp	193	4	higher	high	JJR
cord-333636-h2sg6shp	193	5	molecular	molecular	JJ
cord-333636-h2sg6shp	193	6	mass	mass	NN
cord-333636-h2sg6shp	193	7	species	specie	NNS
cord-333636-h2sg6shp	193	8	migrated	migrate	VBD
cord-333636-h2sg6shp	193	9	according	accord	VBG
cord-333636-h2sg6shp	193	10	to	to	IN
cord-333636-h2sg6shp	193	11	their	-PRON-	PRP$
cord-333636-h2sg6shp	193	12	expected	expect	VBN
cord-333636-h2sg6shp	193	13	molecular	molecular	JJ
cord-333636-h2sg6shp	193	14	masses	masse	NNS
cord-333636-h2sg6shp	193	15	.	.	.
cord-333636-h2sg6shp	194	1	Estimation	estimation	NN
cord-333636-h2sg6shp	194	2	of	of	IN
cord-333636-h2sg6shp	194	3	the	the	DT
cord-333636-h2sg6shp	194	4	molecular	molecular	JJ
cord-333636-h2sg6shp	194	5	masses	masse	NNS
cord-333636-h2sg6shp	194	6	of	of	IN
cord-333636-h2sg6shp	194	7	the	the	DT
cord-333636-h2sg6shp	194	8	lower	low	JJR
cord-333636-h2sg6shp	194	9	protein	protein	NN
cord-333636-h2sg6shp	194	10	bands	band	NNS
cord-333636-h2sg6shp	194	11	indicated	indicate	VBD
cord-333636-h2sg6shp	194	12	a	a	DT
cord-333636-h2sg6shp	194	13	consistent	consistent	JJ
cord-333636-h2sg6shp	194	14	reduction	reduction	NN
cord-333636-h2sg6shp	194	15	of	of	IN
cord-333636-h2sg6shp	194	16	about	about	RB
cord-333636-h2sg6shp	194	17	1.5	1.5	CD
cord-333636-h2sg6shp	194	18	kDa	kDa	NNS
cord-333636-h2sg6shp	194	19	compared	compare	VBN
cord-333636-h2sg6shp	194	20	to	to	IN
cord-333636-h2sg6shp	194	21	the	the	DT
cord-333636-h2sg6shp	194	22	expected	expect	VBN
cord-333636-h2sg6shp	194	23	size	size	NN
cord-333636-h2sg6shp	194	24	(	(	-LRB-
cord-333636-h2sg6shp	194	25	Fig	Fig	NNP
cord-333636-h2sg6shp	194	26	.	.	.
cord-333636-h2sg6shp	194	27	6B	6b	CD
cord-333636-h2sg6shp	194	28	)	)	-RRB-
cord-333636-h2sg6shp	194	29	.	.	.
cord-333636-h2sg6shp	195	1	Northwestern	northwestern	JJ
cord-333636-h2sg6shp	195	2	analyses	analysis	NNS
cord-333636-h2sg6shp	195	3	showed	show	VBD
cord-333636-h2sg6shp	195	4	that	that	IN
cord-333636-h2sg6shp	195	5	both	both	DT
cord-333636-h2sg6shp	195	6	wild	wild	JJ
cord-333636-h2sg6shp	195	7	-	-	HYPH
cord-333636-h2sg6shp	195	8	type	type	NN
cord-333636-h2sg6shp	195	9	-	-	HYPH
cord-333636-h2sg6shp	195	10	derived	derive	VBN
cord-333636-h2sg6shp	195	11	VP3	VP3	NNP
cord-333636-h2sg6shp	195	12	forms	form	NNS
cord-333636-h2sg6shp	195	13	interacted	interact	VBN
cord-333636-h2sg6shp	195	14	with	with	IN
cord-333636-h2sg6shp	195	15	labeled	label	VBN
cord-333636-h2sg6shp	195	16	ssRNA	ssrna	NN
cord-333636-h2sg6shp	195	17	probes	probe	NNS
cord-333636-h2sg6shp	195	18	(	(	-LRB-
cord-333636-h2sg6shp	195	19	data	datum	NNS
cord-333636-h2sg6shp	195	20	not	not	RB
cord-333636-h2sg6shp	195	21	shown	show	VBN
cord-333636-h2sg6shp	195	22	)	)	-RRB-
cord-333636-h2sg6shp	195	23	.	.	.
cord-333636-h2sg6shp	196	1	The	the	DT
cord-333636-h2sg6shp	196	2	affinity	affinity	NN
cord-333636-h2sg6shp	196	3	of	of	IN
cord-333636-h2sg6shp	196	4	each	each	DT
cord-333636-h2sg6shp	196	5	mutant	mutant	JJ
cord-333636-h2sg6shp	196	6	protein	protein	NN
cord-333636-h2sg6shp	196	7	to	to	IN
cord-333636-h2sg6shp	196	8	ssRNA	ssRNA	VBG
cord-333636-h2sg6shp	196	9	was	be	VBD
cord-333636-h2sg6shp	196	10	determined	determine	VBN
cord-333636-h2sg6shp	196	11	by	by	IN
cord-333636-h2sg6shp	196	12	membrane	membrane	NN
cord-333636-h2sg6shp	196	13	filter	filter	NN
cord-333636-h2sg6shp	196	14	binding	bind	VBG
cord-333636-h2sg6shp	196	15	assays	assay	NNS
cord-333636-h2sg6shp	196	16	,	,	,
cord-333636-h2sg6shp	196	17	using	use	VBG
cord-333636-h2sg6shp	196	18	probe	probe	NN
cord-333636-h2sg6shp	196	19	B	B	NNP
cord-333636-h2sg6shp	196	20	,	,	,
cord-333636-h2sg6shp	196	21	as	as	IN
cord-333636-h2sg6shp	196	22	described	describe	VBN
cord-333636-h2sg6shp	196	23	above	above	RB
cord-333636-h2sg6shp	196	24	.	.	.
cord-333636-h2sg6shp	197	1	The	the	DT
cord-333636-h2sg6shp	197	2	amounts	amount	NNS
cord-333636-h2sg6shp	197	3	of	of	IN
cord-333636-h2sg6shp	197	4	the	the	DT
cord-333636-h2sg6shp	197	5	purified	purify	VBN
cord-333636-h2sg6shp	197	6	deletion	deletion	NN
cord-333636-h2sg6shp	197	7	mutant	mutant	NN
cord-333636-h2sg6shp	197	8	proteins	protein	NNS
cord-333636-h2sg6shp	197	9	used	use	VBN
cord-333636-h2sg6shp	197	10	in	in	IN
cord-333636-h2sg6shp	197	11	these	these	DT
cord-333636-h2sg6shp	197	12	assays	assay	NNS
cord-333636-h2sg6shp	197	13	were	be	VBD
cord-333636-h2sg6shp	197	14	normalized	normalize	VBN
cord-333636-h2sg6shp	197	15	according	accord	VBG
cord-333636-h2sg6shp	197	16	to	to	IN
cord-333636-h2sg6shp	197	17	their	-PRON-	PRP$
cord-333636-h2sg6shp	197	18	respective	respective	JJ
cord-333636-h2sg6shp	197	19	molecular	molecular	JJ
cord-333636-h2sg6shp	197	20	masses	masse	NNS
cord-333636-h2sg6shp	197	21	.	.	.
cord-333636-h2sg6shp	198	1	Identical	identical	JJ
cord-333636-h2sg6shp	198	2	molar	molar	JJ
cord-333636-h2sg6shp	198	3	amounts	amount	NNS
cord-333636-h2sg6shp	198	4	of	of	IN
cord-333636-h2sg6shp	198	5	each	each	DT
cord-333636-h2sg6shp	198	6	mutant	mutant	JJ
cord-333636-h2sg6shp	198	7	protein	protein	NN
cord-333636-h2sg6shp	198	8	were	be	VBD
cord-333636-h2sg6shp	198	9	used	use	VBN
cord-333636-h2sg6shp	198	10	in	in	IN
cord-333636-h2sg6shp	198	11	the	the	DT
cord-333636-h2sg6shp	198	12	assays	assay	NNS
cord-333636-h2sg6shp	198	13	.	.	.
cord-333636-h2sg6shp	199	1	The	the	DT
cord-333636-h2sg6shp	199	2	ssRNA	ssrna	RB
cord-333636-h2sg6shp	199	3	-	-	HYPH
cord-333636-h2sg6shp	199	4	binding	bind	VBG
cord-333636-h2sg6shp	199	5	activity	activity	NN
cord-333636-h2sg6shp	199	6	of	of	IN
cord-333636-h2sg6shp	199	7	WT	WT	NNP
cord-333636-h2sg6shp	199	8	VP3	VP3	NNP
cord-333636-h2sg6shp	199	9	was	be	VBD
cord-333636-h2sg6shp	199	10	estimated	estimate	VBN
cord-333636-h2sg6shp	199	11	to	to	TO
cord-333636-h2sg6shp	199	12	have	have	VB
cord-333636-h2sg6shp	199	13	an	an	DT
cord-333636-h2sg6shp	199	14	apparent	apparent	JJ
cord-333636-h2sg6shp	199	15	Kd	Kd	NNP
cord-333636-h2sg6shp	199	16	of	of	IN
cord-333636-h2sg6shp	199	17	(	(	-LRB-
cord-333636-h2sg6shp	199	18	2	2	CD
cord-333636-h2sg6shp	199	19	±	±	CD
cord-333636-h2sg6shp	199	20	0.2	0.2	CD
cord-333636-h2sg6shp	199	21	)	)	-RRB-
cord-333636-h2sg6shp	199	22	×	×	NN
cord-333636-h2sg6shp	199	23	10	10	CD
cord-333636-h2sg6shp	200	1	−4	−4	NNP
cord-333636-h2sg6shp	200	2	mM	mm	NN
cord-333636-h2sg6shp	200	3	(	(	-LRB-
cord-333636-h2sg6shp	200	4	n	n	NN
cord-333636-h2sg6shp	200	5	=	=	SYM
cord-333636-h2sg6shp	200	6	3	3	CD
cord-333636-h2sg6shp	200	7	,	,	,
cord-333636-h2sg6shp	200	8	CV	cv	NN
cord-333636-h2sg6shp	200	9	=	=	SYM
cord-333636-h2sg6shp	200	10	10	10	CD
cord-333636-h2sg6shp	200	11	%	%	NN
cord-333636-h2sg6shp	200	12	)	)	-RRB-
cord-333636-h2sg6shp	201	1	(	(	-LRB-
cord-333636-h2sg6shp	201	2	Figs	fig	NNS
cord-333636-h2sg6shp	201	3	.	.	.
cord-333636-h2sg6shp	202	1	3	3	CD
cord-333636-h2sg6shp	202	2	and	and	CC
cord-333636-h2sg6shp	202	3	7	7	CD
cord-333636-h2sg6shp	202	4	)	)	-RRB-
cord-333636-h2sg6shp	202	5	.	.	.
cord-333636-h2sg6shp	203	1	Deletion	deletion	NN
cord-333636-h2sg6shp	203	2	mutants	mutant	NNS
cord-333636-h2sg6shp	203	3	VP3	vp3	VBP
cord-333636-h2sg6shp	203	4	756	756	CD
cord-333636-h2sg6shp	203	5	-	-	HYPH
cord-333636-h2sg6shp	203	6	780	780	CD
cord-333636-h2sg6shp	203	7	and	and	CC
cord-333636-h2sg6shp	203	8	VP3	VP3	NNP
cord-333636-h2sg6shp	203	9	781	781	CD
cord-333636-h2sg6shp	203	10	-	-	HYPH
cord-333636-h2sg6shp	203	11	807	807	CD
cord-333636-h2sg6shp	203	12	presented	present	VBD
cord-333636-h2sg6shp	203	13	a	a	DT
cord-333636-h2sg6shp	203	14	slightly	slightly	RB
cord-333636-h2sg6shp	203	15	reduced	reduce	VBN
cord-333636-h2sg6shp	203	16	affinity	affinity	NN
cord-333636-h2sg6shp	203	17	compared	compare	VBN
cord-333636-h2sg6shp	203	18	to	to	IN
cord-333636-h2sg6shp	203	19	that	that	DT
cord-333636-h2sg6shp	203	20	of	of	IN
cord-333636-h2sg6shp	203	21	WT	WT	NNP
cord-333636-h2sg6shp	203	22	VP3	VP3	NNP
cord-333636-h2sg6shp	203	23	.	.	.
cord-333636-h2sg6shp	204	1	Carboxy	carboxy	JJ
cord-333636-h2sg6shp	204	2	-	-	HYPH
cord-333636-h2sg6shp	204	3	terminal	terminal	NN
cord-333636-h2sg6shp	204	4	deletion	deletion	NN
cord-333636-h2sg6shp	204	5	mutants	mutant	NNS
cord-333636-h2sg6shp	204	6	showed	show	VBD
cord-333636-h2sg6shp	204	7	an	an	DT
cord-333636-h2sg6shp	204	8	affinity	affinity	NN
cord-333636-h2sg6shp	204	9	similar	similar	JJ
cord-333636-h2sg6shp	204	10	to	to	IN
cord-333636-h2sg6shp	204	11	that	that	DT
cord-333636-h2sg6shp	204	12	of	of	IN
cord-333636-h2sg6shp	204	13	the	the	DT
cord-333636-h2sg6shp	204	14	complete	complete	JJ
cord-333636-h2sg6shp	204	15	VP3	VP3	NNP
cord-333636-h2sg6shp	204	16	protein	protein	NN
cord-333636-h2sg6shp	204	17	,	,	,
cord-333636-h2sg6shp	204	18	indicating	indicate	VBG
cord-333636-h2sg6shp	204	19	that	that	IN
cord-333636-h2sg6shp	204	20	the	the	DT
cord-333636-h2sg6shp	204	21	ssRNA	ssrna	RB
cord-333636-h2sg6shp	204	22	-	-	HYPH
cord-333636-h2sg6shp	204	23	binding	bind	VBG
cord-333636-h2sg6shp	204	24	domain	domain	NN
cord-333636-h2sg6shp	204	25	was	be	VBD
cord-333636-h2sg6shp	204	26	not	not	RB
cord-333636-h2sg6shp	204	27	affected	affect	VBN
cord-333636-h2sg6shp	204	28	by	by	IN
cord-333636-h2sg6shp	204	29	these	these	DT
cord-333636-h2sg6shp	204	30	deletions	deletion	NNS
cord-333636-h2sg6shp	204	31	(	(	-LRB-
cord-333636-h2sg6shp	204	32	Fig	Fig	NNP
cord-333636-h2sg6shp	204	33	.	.	NNP
cord-333636-h2sg6shp	204	34	7	7	CD
cord-333636-h2sg6shp	204	35	)	)	-RRB-
cord-333636-h2sg6shp	204	36	.	.	.
cord-333636-h2sg6shp	205	1	In	in	IN
cord-333636-h2sg6shp	205	2	contrast	contrast	NN
cord-333636-h2sg6shp	205	3	,	,	,
cord-333636-h2sg6shp	205	4	mutants	mutant	NNS
cord-333636-h2sg6shp	205	5	presenting	present	VBG
cord-333636-h2sg6shp	205	6	deletions	deletion	NNS
cord-333636-h2sg6shp	205	7	at	at	IN
cord-333636-h2sg6shp	205	8	the	the	DT
cord-333636-h2sg6shp	205	9	region	region	NN
cord-333636-h2sg6shp	205	10	between	between	IN
cord-333636-h2sg6shp	205	11	residues	residue	NNS
cord-333636-h2sg6shp	205	12	808	808	CD
cord-333636-h2sg6shp	205	13	and	and	CC
cord-333636-h2sg6shp	205	14	879	879	CD
cord-333636-h2sg6shp	205	15	showed	show	VBD
cord-333636-h2sg6shp	205	16	significantly	significantly	RB
cord-333636-h2sg6shp	205	17	lower	low	JJR
cord-333636-h2sg6shp	205	18	affinities	affinity	NNS
cord-333636-h2sg6shp	205	19	to	to	IN
cord-333636-h2sg6shp	205	20	ssRNA	ssrna	VB
cord-333636-h2sg6shp	205	21	,	,	,
cord-333636-h2sg6shp	205	22	up	up	IN
cord-333636-h2sg6shp	205	23	to	to	TO
cord-333636-h2sg6shp	205	24	100-fold	100-fold	CD
cord-333636-h2sg6shp	205	25	reduction	reduction	NN
cord-333636-h2sg6shp	205	26	in	in	IN
cord-333636-h2sg6shp	205	27	the	the	DT
cord-333636-h2sg6shp	205	28	case	case	NN
cord-333636-h2sg6shp	205	29	of	of	IN
cord-333636-h2sg6shp	205	30	VP3	VP3	NNP
cord-333636-h2sg6shp	205	31	856	856	CD
cord-333636-h2sg6shp	205	32	-	-	SYM
cord-333636-h2sg6shp	205	33	879	879	CD
cord-333636-h2sg6shp	205	34	deletion	deletion	NN
cord-333636-h2sg6shp	205	35	mutant	mutant	NN
cord-333636-h2sg6shp	205	36	.	.	.
cord-333636-h2sg6shp	206	1	These	these	DT
cord-333636-h2sg6shp	206	2	results	result	NNS
cord-333636-h2sg6shp	206	3	indicated	indicate	VBD
cord-333636-h2sg6shp	206	4	that	that	IN
cord-333636-h2sg6shp	206	5	the	the	DT
cord-333636-h2sg6shp	206	6	VP3	VP3	NNP
cord-333636-h2sg6shp	206	7	ssRNA	ssrna	RB
cord-333636-h2sg6shp	206	8	-	-	HYPH
cord-333636-h2sg6shp	206	9	binding	bind	VBG
cord-333636-h2sg6shp	206	10	domain	domain	NN
cord-333636-h2sg6shp	206	11	was	be	VBD
cord-333636-h2sg6shp	206	12	located	locate	VBN
cord-333636-h2sg6shp	206	13	within	within	IN
cord-333636-h2sg6shp	206	14	a	a	DT
cord-333636-h2sg6shp	206	15	region	region	NN
cord-333636-h2sg6shp	206	16	comprising	comprise	VBG
cord-333636-h2sg6shp	206	17	from	from	IN
cord-333636-h2sg6shp	206	18	residues	residue	NNS
cord-333636-h2sg6shp	206	19	808	808	CD
cord-333636-h2sg6shp	206	20	to	to	IN
cord-333636-h2sg6shp	206	21	879	879	CD
cord-333636-h2sg6shp	206	22	(	(	-LRB-
cord-333636-h2sg6shp	206	23	Fig	Fig	NNP
cord-333636-h2sg6shp	206	24	.	.	.
cord-333636-h2sg6shp	206	25	7B	7B	NNP
cord-333636-h2sg6shp	206	26	)	)	-RRB-
cord-333636-h2sg6shp	206	27	.	.	.
cord-333636-h2sg6shp	207	1	To	to	TO
cord-333636-h2sg6shp	207	2	investigate	investigate	VB
cord-333636-h2sg6shp	207	3	whether	whether	IN
cord-333636-h2sg6shp	207	4	the	the	DT
cord-333636-h2sg6shp	207	5	identified	identify	VBN
cord-333636-h2sg6shp	207	6	ssRNA	ssrna	RB
cord-333636-h2sg6shp	207	7	-	-	HYPH
cord-333636-h2sg6shp	207	8	binding	bind	VBG
cord-333636-h2sg6shp	207	9	domain	domain	NN
cord-333636-h2sg6shp	207	10	presented	present	VBD
cord-333636-h2sg6shp	207	11	highly	highly	RB
cord-333636-h2sg6shp	207	12	conserved	conserve	VBN
cord-333636-h2sg6shp	207	13	sequence	sequence	NN
cord-333636-h2sg6shp	207	14	features	feature	NNS
cord-333636-h2sg6shp	207	15	,	,	,
cord-333636-h2sg6shp	207	16	a	a	DT
cord-333636-h2sg6shp	207	17	comparison	comparison	NN
cord-333636-h2sg6shp	207	18	was	be	VBD
cord-333636-h2sg6shp	207	19	performed	perform	VBN
cord-333636-h2sg6shp	207	20	using	use	VBG
cord-333636-h2sg6shp	207	21	the	the	DT
cord-333636-h2sg6shp	207	22	polyprotein	polyprotein	NNP
cord-333636-h2sg6shp	207	23	sequences	sequence	NNS
cord-333636-h2sg6shp	207	24	of	of	IN
cord-333636-h2sg6shp	207	25	representative	representative	JJ
cord-333636-h2sg6shp	207	26	species	specie	NNS
cord-333636-h2sg6shp	207	27	from	from	IN
cord-333636-h2sg6shp	207	28	the	the	DT
cord-333636-h2sg6shp	207	29	family	family	NN
cord-333636-h2sg6shp	207	30	Birnaviridae	Birnaviridae	NNP
cord-333636-h2sg6shp	207	31	.	.	.
cord-333636-h2sg6shp	208	1	The	the	DT
cord-333636-h2sg6shp	208	2	alignment	alignment	NN
cord-333636-h2sg6shp	208	3	and	and	CC
cord-333636-h2sg6shp	208	4	comparison	comparison	NN
cord-333636-h2sg6shp	208	5	of	of	IN
cord-333636-h2sg6shp	208	6	VP3	VP3	NNP
cord-333636-h2sg6shp	208	7	protein	protein	NN
cord-333636-h2sg6shp	208	8	sequences	sequence	NNS
cord-333636-h2sg6shp	208	9	revealed	reveal	VBD
cord-333636-h2sg6shp	208	10	that	that	IN
cord-333636-h2sg6shp	208	11	in	in	IN
cord-333636-h2sg6shp	208	12	the	the	DT
cord-333636-h2sg6shp	208	13	region	region	NN
cord-333636-h2sg6shp	208	14	implicated	implicate	VBN
cord-333636-h2sg6shp	208	15	in	in	IN
cord-333636-h2sg6shp	208	16	RNA	RNA	NNP
cord-333636-h2sg6shp	208	17	-	-	HYPH
cord-333636-h2sg6shp	208	18	binding	binding	NN
cord-333636-h2sg6shp	208	19	,	,	,
cord-333636-h2sg6shp	208	20	strains	strain	NNS
cord-333636-h2sg6shp	208	21	from	from	IN
cord-333636-h2sg6shp	208	22	the	the	DT
cord-333636-h2sg6shp	208	23	genus	genus	NN
cord-333636-h2sg6shp	209	1	Avibirnavirus	Avibirnavirus	NNP
cord-333636-h2sg6shp	209	2	contained	contain	VBD
cord-333636-h2sg6shp	209	3	a	a	DT
cord-333636-h2sg6shp	209	4	14	14	CD
cord-333636-h2sg6shp	209	5	aa	aa	NNP
cord-333636-h2sg6shp	209	6	sequence	sequence	NN
cord-333636-h2sg6shp	209	7	absent	absent	JJ
cord-333636-h2sg6shp	209	8	in	in	IN
cord-333636-h2sg6shp	209	9	their	-PRON-	PRP$
cord-333636-h2sg6shp	209	10	counterparts	counterpart	NNS
cord-333636-h2sg6shp	209	11	from	from	IN
cord-333636-h2sg6shp	209	12	the	the	DT
cord-333636-h2sg6shp	209	13	aquabirnavirus	aquabirnavirus	NNP
cord-333636-h2sg6shp	209	14	genus	genus	NN
cord-333636-h2sg6shp	209	15	(	(	-LRB-
cord-333636-h2sg6shp	209	16	Fig	Fig	NNP
cord-333636-h2sg6shp	209	17	.	.	.
cord-333636-h2sg6shp	209	18	8	8	CD
cord-333636-h2sg6shp	209	19	)	)	-RRB-
cord-333636-h2sg6shp	209	20	.	.	.
cord-333636-h2sg6shp	210	1	In	in	IN
cord-333636-h2sg6shp	210	2	addition	addition	NN
cord-333636-h2sg6shp	210	3	,	,	,
cord-333636-h2sg6shp	210	4	sequence	sequence	NN
cord-333636-h2sg6shp	210	5	comparisons	comparison	NNS
cord-333636-h2sg6shp	210	6	showed	show	VBD
cord-333636-h2sg6shp	210	7	two	two	CD
cord-333636-h2sg6shp	210	8	absolutely	absolutely	RB
cord-333636-h2sg6shp	210	9	conserved	conserved	JJ
cord-333636-h2sg6shp	210	10	residues	residue	NNS
cord-333636-h2sg6shp	210	11	,	,	,
cord-333636-h2sg6shp	210	12	Arg	arg	NN
cord-333636-h2sg6shp	210	13	858	858	CD
cord-333636-h2sg6shp	210	14	and	and	CC
cord-333636-h2sg6shp	210	15	Gly	Gly	NNP
cord-333636-h2sg6shp	210	16	867	867	CD
cord-333636-h2sg6shp	210	17	,	,	,
cord-333636-h2sg6shp	210	18	within	within	IN
cord-333636-h2sg6shp	210	19	this	this	DT
cord-333636-h2sg6shp	210	20	region	region	NN
cord-333636-h2sg6shp	210	21	.	.	.
cord-333636-h2sg6shp	211	1	A	a	DT
cord-333636-h2sg6shp	211	2	highly	highly	RB
cord-333636-h2sg6shp	211	3	hydrophobic	hydrophobic	JJ
cord-333636-h2sg6shp	211	4	region	region	NN
cord-333636-h2sg6shp	211	5	,	,	,
cord-333636-h2sg6shp	211	6	including	include	VBG
cord-333636-h2sg6shp	211	7	the	the	DT
cord-333636-h2sg6shp	211	8	conserved	conserve	VBN
cord-333636-h2sg6shp	211	9	Gly	Gly	NNP
cord-333636-h2sg6shp	211	10	867	867	CD
cord-333636-h2sg6shp	211	11	(	(	-LRB-
cord-333636-h2sg6shp	211	12	G	G	NNP
cord-333636-h2sg6shp	211	13	-	-	HYPH
cord-333636-h2sg6shp	211	14	FA	fa	NN
cord-333636-h2sg6shp	211	15	-	-	HYPH
cord-333636-h2sg6shp	211	16	P	P	NNP
cord-333636-h2sg6shp	211	17	-	-	HYPH
cord-333636-h2sg6shp	211	18	W	w	NN
cord-333636-h2sg6shp	211	19	-	-	HYPH
cord-333636-h2sg6shp	211	20	A	A	NNP
cord-333636-h2sg6shp	211	21	-	-	HYPH
cord-333636-h2sg6shp	211	22	N	N	NNP
cord-333636-h2sg6shp	211	23	)	)	-RRB-
cord-333636-h2sg6shp	211	24	was	be	VBD
cord-333636-h2sg6shp	211	25	also	also	RB
cord-333636-h2sg6shp	211	26	found	find	VBN
cord-333636-h2sg6shp	211	27	between	between	IN
cord-333636-h2sg6shp	211	28	residues	residue	NNS
cord-333636-h2sg6shp	211	29	867	867	CD
cord-333636-h2sg6shp	211	30	and	and	CC
cord-333636-h2sg6shp	211	31	879	879	CD
cord-333636-h2sg6shp	212	1	(	(	-LRB-
cord-333636-h2sg6shp	212	2	Fig	Fig	NNP
cord-333636-h2sg6shp	212	3	.	.	.
cord-333636-h2sg6shp	212	4	8)	8)	NNP
cord-333636-h2sg6shp	212	5	.	.	.
cord-333636-h2sg6shp	213	1	Interestingly	interestingly	RB
cord-333636-h2sg6shp	213	2	,	,	,
cord-333636-h2sg6shp	213	3	when	when	WRB
cord-333636-h2sg6shp	213	4	this	this	DT
cord-333636-h2sg6shp	213	5	region	region	NN
cord-333636-h2sg6shp	213	6	was	be	VBD
cord-333636-h2sg6shp	213	7	deleted	delete	VBN
cord-333636-h2sg6shp	213	8	from	from	IN
cord-333636-h2sg6shp	213	9	VP3	VP3	NNP
cord-333636-h2sg6shp	213	10	(	(	-LRB-
cord-333636-h2sg6shp	213	11	VP3	VP3	NNP
cord-333636-h2sg6shp	213	12	856	856	CD
cord-333636-h2sg6shp	213	13	-	-	HYPH
cord-333636-h2sg6shp	213	14	879	879	CD
cord-333636-h2sg6shp	213	15	)	)	-RRB-
cord-333636-h2sg6shp	213	16	,	,	,
cord-333636-h2sg6shp	213	17	the	the	DT
cord-333636-h2sg6shp	213	18	ssRNA	ssrna	RB
cord-333636-h2sg6shp	213	19	-	-	HYPH
cord-333636-h2sg6shp	213	20	binding	bind	VBG
cord-333636-h2sg6shp	213	21	activity	activity	NN
cord-333636-h2sg6shp	213	22	was	be	VBD
cord-333636-h2sg6shp	213	23	most	most	RBS
cord-333636-h2sg6shp	213	24	severely	severely	RB
cord-333636-h2sg6shp	213	25	impaired	impaired	JJ
cord-333636-h2sg6shp	213	26	(	(	-LRB-
cord-333636-h2sg6shp	213	27	Fig	Fig	NNP
cord-333636-h2sg6shp	213	28	.	.	NNP
cord-333636-h2sg6shp	213	29	7	7	CD
cord-333636-h2sg6shp	213	30	)	)	-RRB-
cord-333636-h2sg6shp	213	31	,	,	,
cord-333636-h2sg6shp	213	32	suggesting	suggest	VBG
cord-333636-h2sg6shp	213	33	that	that	IN
cord-333636-h2sg6shp	213	34	this	this	DT
cord-333636-h2sg6shp	213	35	region	region	NN
cord-333636-h2sg6shp	213	36	might	may	MD
cord-333636-h2sg6shp	213	37	play	play	VB
cord-333636-h2sg6shp	213	38	a	a	DT
cord-333636-h2sg6shp	213	39	critical	critical	JJ
cord-333636-h2sg6shp	213	40	role	role	NN
cord-333636-h2sg6shp	213	41	for	for	IN
cord-333636-h2sg6shp	213	42	ssRNA	ssrna	RB
cord-333636-h2sg6shp	213	43	-	-	HYPH
cord-333636-h2sg6shp	213	44	binding	bind	VBG
cord-333636-h2sg6shp	213	45	.	.	.
cord-333636-h2sg6shp	214	1	Other	other	JJ
cord-333636-h2sg6shp	214	2	highly	highly	RB
cord-333636-h2sg6shp	214	3	conserved	conserved	JJ
cord-333636-h2sg6shp	214	4	short	short	JJ
cord-333636-h2sg6shp	214	5	sequences	sequence	NNS
cord-333636-h2sg6shp	214	6	were	be	VBD
cord-333636-h2sg6shp	214	7	also	also	RB
cord-333636-h2sg6shp	214	8	found	find	VBN
cord-333636-h2sg6shp	214	9	,	,	,
cord-333636-h2sg6shp	214	10	but	but	CC
cord-333636-h2sg6shp	214	11	RNA	RNA	NNP
cord-333636-h2sg6shp	214	12	or	or	CC
cord-333636-h2sg6shp	214	13	DNA	dna	NN
cord-333636-h2sg6shp	214	14	binding	binding	NN
cord-333636-h2sg6shp	214	15	motifs	motif	NNS
cord-333636-h2sg6shp	214	16	previously	previously	RB
cord-333636-h2sg6shp	214	17	described	describe	VBN
cord-333636-h2sg6shp	214	18	in	in	IN
cord-333636-h2sg6shp	214	19	the	the	DT
cord-333636-h2sg6shp	214	20	literature	literature	NN
cord-333636-h2sg6shp	214	21	such	such	JJ
cord-333636-h2sg6shp	214	22	as	as	IN
cord-333636-h2sg6shp	214	23	zinc	zinc	NN
cord-333636-h2sg6shp	214	24	fingers	finger	NNS
cord-333636-h2sg6shp	214	25	,	,	,
cord-333636-h2sg6shp	214	26	arginine	arginine	NN
cord-333636-h2sg6shp	214	27	-	-	HYPH
cord-333636-h2sg6shp	214	28	rich	rich	JJ
cord-333636-h2sg6shp	214	29	regions	region	NNS
cord-333636-h2sg6shp	214	30	or	or	CC
cord-333636-h2sg6shp	214	31	RG	RG	NNP
cord-333636-h2sg6shp	214	32	boxes	box	NNS
cord-333636-h2sg6shp	214	33	,	,	,
cord-333636-h2sg6shp	214	34	were	be	VBD
cord-333636-h2sg6shp	214	35	not	not	RB
cord-333636-h2sg6shp	214	36	found	find	VBN
cord-333636-h2sg6shp	214	37	within	within	IN
cord-333636-h2sg6shp	214	38	VP3	VP3	NNP
cord-333636-h2sg6shp	214	39	[	[	-LRB-
cord-333636-h2sg6shp	214	40	8	8	CD
cord-333636-h2sg6shp	214	41	]	]	-RRB-
cord-333636-h2sg6shp	214	42	.	.	.
cord-333636-h2sg6shp	215	1	Whether	whether	IN
cord-333636-h2sg6shp	215	2	this	this	DT
cord-333636-h2sg6shp	215	3	region	region	NN
cord-333636-h2sg6shp	215	4	contains	contain	VBZ
cord-333636-h2sg6shp	215	5	a	a	DT
cord-333636-h2sg6shp	215	6	previously	previously	RB
cord-333636-h2sg6shp	215	7	unpublished	unpublished	JJ
cord-333636-h2sg6shp	215	8	RNA	rna	NN
cord-333636-h2sg6shp	215	9	-	-	HYPH
cord-333636-h2sg6shp	215	10	binding	bind	VBG
cord-333636-h2sg6shp	215	11	motif	motif	NN
cord-333636-h2sg6shp	215	12	,	,	,
cord-333636-h2sg6shp	215	13	or	or	CC
cord-333636-h2sg6shp	215	14	if	if	IN
cord-333636-h2sg6shp	215	15	it	-PRON-	PRP
cord-333636-h2sg6shp	215	16	comprises	comprise	VBZ
cord-333636-h2sg6shp	215	17	a	a	DT
cord-333636-h2sg6shp	215	18	double	double	RB
cord-333636-h2sg6shp	215	19	-	-	HYPH
cord-333636-h2sg6shp	215	20	stranded	strand	VBN
cord-333636-h2sg6shp	215	21	RNA	rna	NN
cord-333636-h2sg6shp	215	22	-	-	HYPH
cord-333636-h2sg6shp	215	23	binding	bind	VBG
cord-333636-h2sg6shp	215	24	motif	motif	NN
cord-333636-h2sg6shp	215	25	[	[	-LRB-
cord-333636-h2sg6shp	215	26	8	8	CD
cord-333636-h2sg6shp	215	27	]	]	-RRB-
cord-333636-h2sg6shp	215	28	is	be	VBZ
cord-333636-h2sg6shp	215	29	currently	currently	RB
cord-333636-h2sg6shp	215	30	under	under	IN
cord-333636-h2sg6shp	215	31	investigation	investigation	NN
cord-333636-h2sg6shp	215	32	.	.	.
cord-333636-h2sg6shp	216	1	A	a	DT
cord-333636-h2sg6shp	216	2	detailed	detailed	JJ
cord-333636-h2sg6shp	216	3	mutagenesis	mutagenesis	NN
cord-333636-h2sg6shp	216	4	study	study	NN
cord-333636-h2sg6shp	216	5	is	be	VBZ
cord-333636-h2sg6shp	216	6	being	be	VBG
cord-333636-h2sg6shp	216	7	carried	carry	VBN
cord-333636-h2sg6shp	216	8	out	out	RP
cord-333636-h2sg6shp	216	9	to	to	TO
cord-333636-h2sg6shp	216	10	identify	identify	VB
cord-333636-h2sg6shp	216	11	which	which	WDT
cord-333636-h2sg6shp	216	12	of	of	IN
cord-333636-h2sg6shp	216	13	the	the	DT
cord-333636-h2sg6shp	216	14	conserved	conserve	VBN
cord-333636-h2sg6shp	216	15	regions	region	NNS
cord-333636-h2sg6shp	216	16	in	in	IN
cord-333636-h2sg6shp	216	17	the	the	DT
cord-333636-h2sg6shp	216	18	RNA	RNA	NNP
cord-333636-h2sg6shp	216	19	-	-	HYPH
cord-333636-h2sg6shp	216	20	binding	bind	VBG
cord-333636-h2sg6shp	216	21	domain	domain	NN
cord-333636-h2sg6shp	216	22	is	be	VBZ
cord-333636-h2sg6shp	216	23	directly	directly	RB
cord-333636-h2sg6shp	216	24	involved	involve	VBN
cord-333636-h2sg6shp	216	25	in	in	IN
cord-333636-h2sg6shp	216	26	RNA	rna	NN
cord-333636-h2sg6shp	216	27	-	-	HYPH
cord-333636-h2sg6shp	216	28	binding	binding	NN
cord-333636-h2sg6shp	216	29	.	.	.
cord-333636-h2sg6shp	217	1	Capsids	Capsids	NNP
cord-333636-h2sg6shp	217	2	of	of	IN
cord-333636-h2sg6shp	217	3	dsRNA	dsRNA	``
cord-333636-h2sg6shp	217	4	viruses	virus	NNS
cord-333636-h2sg6shp	217	5	having	have	VBG
cord-333636-h2sg6shp	217	6	a	a	DT
cord-333636-h2sg6shp	217	7	life	life	NN
cord-333636-h2sg6shp	217	8	cycle	cycle	NN
cord-333636-h2sg6shp	217	9	including	include	VBG
cord-333636-h2sg6shp	217	10	an	an	DT
cord-333636-h2sg6shp	217	11	extracellular	extracellular	JJ
cord-333636-h2sg6shp	217	12	phase	phase	NN
cord-333636-h2sg6shp	217	13	contain	contain	VBP
cord-333636-h2sg6shp	217	14	two	two	CD
cord-333636-h2sg6shp	217	15	or	or	CC
cord-333636-h2sg6shp	217	16	more	more	JJR
cord-333636-h2sg6shp	217	17	protein	protein	NN
cord-333636-h2sg6shp	217	18	layers	layer	NNS
cord-333636-h2sg6shp	217	19	[	[	-LRB-
cord-333636-h2sg6shp	217	20	2	2	CD
cord-333636-h2sg6shp	217	21	,	,	,
cord-333636-h2sg6shp	217	22	9	9	CD
cord-333636-h2sg6shp	217	23	]	]	-RRB-
cord-333636-h2sg6shp	217	24	.	.	.
cord-333636-h2sg6shp	218	1	Strikingly	strikingly	RB
cord-333636-h2sg6shp	218	2	,	,	,
cord-333636-h2sg6shp	218	3	capsids	capsid	NNS
cord-333636-h2sg6shp	218	4	of	of	IN
cord-333636-h2sg6shp	218	5	members	member	NNS
cord-333636-h2sg6shp	218	6	of	of	IN
cord-333636-h2sg6shp	218	7	the	the	DT
cord-333636-h2sg6shp	218	8	Birnaviridae	Birnaviridae	NNP
cord-333636-h2sg6shp	218	9	family	family	NN
cord-333636-h2sg6shp	218	10	are	be	VBP
cord-333636-h2sg6shp	218	11	much	much	RB
cord-333636-h2sg6shp	218	12	simpler	simple	JJR
cord-333636-h2sg6shp	218	13	.	.	.
cord-333636-h2sg6shp	219	1	They	-PRON-	PRP
cord-333636-h2sg6shp	219	2	contain	contain	VBP
cord-333636-h2sg6shp	219	3	a	a	DT
cord-333636-h2sg6shp	219	4	single	single	JJ
cord-333636-h2sg6shp	219	5	protein	protein	NN
cord-333636-h2sg6shp	219	6	shell	shell	NN
cord-333636-h2sg6shp	219	7	[	[	-LRB-
cord-333636-h2sg6shp	219	8	6	6	CD
cord-333636-h2sg6shp	219	9	,	,	,
cord-333636-h2sg6shp	219	10	9	9	CD
cord-333636-h2sg6shp	219	11	]	]	-RRB-
cord-333636-h2sg6shp	219	12	.	.	.
cord-333636-h2sg6shp	220	1	This	this	DT
cord-333636-h2sg6shp	220	2	unique	unique	JJ
cord-333636-h2sg6shp	220	3	feature	feature	NN
cord-333636-h2sg6shp	220	4	makes	make	VBZ
cord-333636-h2sg6shp	220	5	especially	especially	RB
cord-333636-h2sg6shp	220	6	interesting	interesting	JJ
cord-333636-h2sg6shp	220	7	the	the	DT
cord-333636-h2sg6shp	220	8	study	study	NN
cord-333636-h2sg6shp	220	9	of	of	IN
cord-333636-h2sg6shp	220	10	both	both	CC
cord-333636-h2sg6shp	220	11	the	the	DT
cord-333636-h2sg6shp	220	12	molecular	molecular	JJ
cord-333636-h2sg6shp	220	13	architecture	architecture	NN
cord-333636-h2sg6shp	220	14	and	and	CC
cord-333636-h2sg6shp	220	15	the	the	DT
cord-333636-h2sg6shp	220	16	morphogenesis	morphogenesis	NN
cord-333636-h2sg6shp	220	17	of	of	IN
cord-333636-h2sg6shp	220	18	birnaviruses	birnaviruse	NNS
cord-333636-h2sg6shp	220	19	.	.	.
cord-333636-h2sg6shp	221	1	The	the	DT
cord-333636-h2sg6shp	221	2	inner	inner	JJ
cord-333636-h2sg6shp	221	3	surface	surface	NN
cord-333636-h2sg6shp	221	4	of	of	IN
cord-333636-h2sg6shp	221	5	the	the	DT
cord-333636-h2sg6shp	221	6	IBDV	IBDV	NNP
cord-333636-h2sg6shp	221	7	particle	particle	NN
cord-333636-h2sg6shp	221	8	is	be	VBZ
cord-333636-h2sg6shp	221	9	coated	coat	VBN
cord-333636-h2sg6shp	221	10	by	by	IN
cord-333636-h2sg6shp	221	11	200Y	200y	CD
cord-333636-h2sg6shp	221	12	-	-	HYPH
cord-333636-h2sg6shp	221	13	shaped	shape	VBN
cord-333636-h2sg6shp	221	14	VP3	VP3	NNP
cord-333636-h2sg6shp	221	15	trimers	trimer	NNS
cord-333636-h2sg6shp	221	16	in	in	IN
cord-333636-h2sg6shp	221	17	close	close	JJ
cord-333636-h2sg6shp	221	18	contact	contact	NN
cord-333636-h2sg6shp	221	19	with	with	IN
cord-333636-h2sg6shp	221	20	the	the	DT
cord-333636-h2sg6shp	221	21	continuous	continuous	JJ
cord-333636-h2sg6shp	221	22	shell	shell	NN
cord-333636-h2sg6shp	221	23	formed	form	VBN
cord-333636-h2sg6shp	221	24	by	by	IN
cord-333636-h2sg6shp	221	25	VP2	VP2	NNP
cord-333636-h2sg6shp	221	26	[	[	-LRB-
cord-333636-h2sg6shp	221	27	6	6	CD
cord-333636-h2sg6shp	221	28	,	,	,
cord-333636-h2sg6shp	221	29	9	9	CD
cord-333636-h2sg6shp	221	30	]	]	-RRB-
cord-333636-h2sg6shp	221	31	.	.	.
cord-333636-h2sg6shp	222	1	Although	although	IN
cord-333636-h2sg6shp	222	2	the	the	DT
cord-333636-h2sg6shp	222	3	precise	precise	JJ
cord-333636-h2sg6shp	222	4	capsid	capsid	NN
cord-333636-h2sg6shp	222	5	location	location	NN
cord-333636-h2sg6shp	222	6	of	of	IN
cord-333636-h2sg6shp	222	7	the	the	DT
cord-333636-h2sg6shp	222	8	putative	putative	JJ
cord-333636-h2sg6shp	222	9	RdRp	RdRp	NNP
cord-333636-h2sg6shp	222	10	,	,	,
cord-333636-h2sg6shp	222	11	VP1	VP1	NNP
cord-333636-h2sg6shp	222	12	,	,	,
cord-333636-h2sg6shp	222	13	has	have	VBZ
cord-333636-h2sg6shp	222	14	not	not	RB
cord-333636-h2sg6shp	222	15	been	be	VBN
cord-333636-h2sg6shp	222	16	established	establish	VBN
cord-333636-h2sg6shp	222	17	yet	yet	RB
cord-333636-h2sg6shp	222	18	,	,	,
cord-333636-h2sg6shp	222	19	it	-PRON-	PRP
cord-333636-h2sg6shp	222	20	has	have	VBZ
cord-333636-h2sg6shp	222	21	been	be	VBN
cord-333636-h2sg6shp	222	22	shown	show	VBN
cord-333636-h2sg6shp	222	23	that	that	IN
cord-333636-h2sg6shp	222	24	it	-PRON-	PRP
cord-333636-h2sg6shp	222	25	directly	directly	RB
cord-333636-h2sg6shp	222	26	interacts	interact	VBZ
cord-333636-h2sg6shp	222	27	with	with	IN
cord-333636-h2sg6shp	222	28	VP3	VP3	NNP
cord-333636-h2sg6shp	222	29	[	[	-LRB-
cord-333636-h2sg6shp	222	30	28	28	CD
cord-333636-h2sg6shp	222	31	,	,	,
cord-333636-h2sg6shp	222	32	43	43	CD
cord-333636-h2sg6shp	222	33	]	]	-RRB-
cord-333636-h2sg6shp	222	34	.	.	.
cord-333636-h2sg6shp	223	1	Therefore	therefore	RB
cord-333636-h2sg6shp	223	2	,	,	,
cord-333636-h2sg6shp	223	3	VP3	VP3	NNP
cord-333636-h2sg6shp	223	4	appears	appear	VBZ
cord-333636-h2sg6shp	223	5	to	to	TO
cord-333636-h2sg6shp	223	6	play	play	VB
cord-333636-h2sg6shp	223	7	a	a	DT
cord-333636-h2sg6shp	223	8	crucial	crucial	JJ
cord-333636-h2sg6shp	223	9	structural	structural	JJ
cord-333636-h2sg6shp	223	10	role	role	NN
cord-333636-h2sg6shp	223	11	.	.	.
cord-333636-h2sg6shp	224	1	It	-PRON-	PRP
cord-333636-h2sg6shp	224	2	has	have	VBZ
cord-333636-h2sg6shp	224	3	been	be	VBN
cord-333636-h2sg6shp	224	4	suggested	suggest	VBN
cord-333636-h2sg6shp	224	5	that	that	IN
cord-333636-h2sg6shp	224	6	VP3	VP3	NNP
cord-333636-h2sg6shp	224	7	might	may	MD
cord-333636-h2sg6shp	224	8	interact	interact	VB
cord-333636-h2sg6shp	224	9	with	with	IN
cord-333636-h2sg6shp	224	10	the	the	DT
cord-333636-h2sg6shp	224	11	dsRNA	dsrna	NN
cord-333636-h2sg6shp	224	12	genome	genome	NN
cord-333636-h2sg6shp	224	13	segments	segment	NNS
cord-333636-h2sg6shp	224	14	[	[	-LRB-
cord-333636-h2sg6shp	224	15	9	9	CD
cord-333636-h2sg6shp	224	16	,	,	,
cord-333636-h2sg6shp	224	17	22	22	CD
cord-333636-h2sg6shp	224	18	,	,	,
cord-333636-h2sg6shp	224	19	23	23	CD
cord-333636-h2sg6shp	224	20	]	]	-RRB-
cord-333636-h2sg6shp	224	21	.	.	.
cord-333636-h2sg6shp	225	1	Indeed	indeed	RB
cord-333636-h2sg6shp	225	2	,	,	,
cord-333636-h2sg6shp	225	3	its	-PRON-	PRP$
cord-333636-h2sg6shp	225	4	presence	presence	NN
cord-333636-h2sg6shp	225	5	in	in	IN
cord-333636-h2sg6shp	225	6	ribonucleoprotein	ribonucleoprotein	NN
cord-333636-h2sg6shp	225	7	complexes	complex	NNS
cord-333636-h2sg6shp	225	8	isolated	isolate	VBN
cord-333636-h2sg6shp	225	9	from	from	IN
cord-333636-h2sg6shp	225	10	disrupted	disrupt	VBN
cord-333636-h2sg6shp	225	11	IPNV	IPNV	NNP
cord-333636-h2sg6shp	225	12	virions	virion	NNS
cord-333636-h2sg6shp	225	13	[	[	-LRB-
cord-333636-h2sg6shp	225	14	22	22	CD
cord-333636-h2sg6shp	225	15	]	]	-RRB-
cord-333636-h2sg6shp	225	16	,	,	,
cord-333636-h2sg6shp	225	17	makes	make	VBZ
cord-333636-h2sg6shp	225	18	it	-PRON-	PRP
cord-333636-h2sg6shp	225	19	a	a	DT
cord-333636-h2sg6shp	225	20	good	good	JJ
cord-333636-h2sg6shp	225	21	candidate	candidate	NN
cord-333636-h2sg6shp	225	22	for	for	IN
cord-333636-h2sg6shp	225	23	playing	play	VBG
cord-333636-h2sg6shp	225	24	a	a	DT
cord-333636-h2sg6shp	225	25	role	role	NN
cord-333636-h2sg6shp	225	26	in	in	IN
cord-333636-h2sg6shp	225	27	genome	genome	NN
cord-333636-h2sg6shp	225	28	packaging	packaging	NN
cord-333636-h2sg6shp	225	29	and/or	and/or	CC
cord-333636-h2sg6shp	225	30	stabilization	stabilization	NN
cord-333636-h2sg6shp	225	31	.	.	.
cord-333636-h2sg6shp	226	1	It	-PRON-	PRP
cord-333636-h2sg6shp	226	2	was	be	VBD
cord-333636-h2sg6shp	226	3	therefore	therefore	RB
cord-333636-h2sg6shp	226	4	interesting	interesting	JJ
cord-333636-h2sg6shp	226	5	to	to	TO
cord-333636-h2sg6shp	226	6	analyze	analyze	VB
cord-333636-h2sg6shp	226	7	the	the	DT
cord-333636-h2sg6shp	226	8	ability	ability	NN
cord-333636-h2sg6shp	226	9	of	of	IN
cord-333636-h2sg6shp	226	10	VP3	VP3	NNP
cord-333636-h2sg6shp	226	11	to	to	TO
cord-333636-h2sg6shp	226	12	interact	interact	VB
cord-333636-h2sg6shp	226	13	with	with	IN
cord-333636-h2sg6shp	226	14	RNA	RNA	NNP
cord-333636-h2sg6shp	226	15	.	.	.
cord-333636-h2sg6shp	227	1	We	-PRON-	PRP
cord-333636-h2sg6shp	227	2	decided	decide	VBD
cord-333636-h2sg6shp	227	3	to	to	TO
cord-333636-h2sg6shp	227	4	undertake	undertake	VB
cord-333636-h2sg6shp	227	5	this	this	DT
cord-333636-h2sg6shp	227	6	analysis	analysis	NN
cord-333636-h2sg6shp	227	7	using	use	VBG
cord-333636-h2sg6shp	227	8	a	a	DT
cord-333636-h2sg6shp	227	9	histidine	histidine	NN
cord-333636-h2sg6shp	227	10	-	-	HYPH
cord-333636-h2sg6shp	227	11	tagged	tag	VBN
cord-333636-h2sg6shp	227	12	version	version	NN
cord-333636-h2sg6shp	227	13	of	of	IN
cord-333636-h2sg6shp	227	14	the	the	DT
cord-333636-h2sg6shp	227	15	protein	protein	NN
cord-333636-h2sg6shp	227	16	produced	produce	VBN
cord-333636-h2sg6shp	227	17	in	in	IN
cord-333636-h2sg6shp	227	18	insect	insect	NN
cord-333636-h2sg6shp	227	19	cells	cell	NNS
cord-333636-h2sg6shp	227	20	infected	infect	VBN
cord-333636-h2sg6shp	227	21	with	with	IN
cord-333636-h2sg6shp	227	22	rBVs	rbv	NNS
cord-333636-h2sg6shp	227	23	.	.	.
cord-333636-h2sg6shp	228	1	The	the	DT
cord-333636-h2sg6shp	228	2	protein	protein	NN
cord-333636-h2sg6shp	228	3	obtained	obtain	VBD
cord-333636-h2sg6shp	228	4	under	under	IN
cord-333636-h2sg6shp	228	5	these	these	DT
cord-333636-h2sg6shp	228	6	conditions	condition	NNS
cord-333636-h2sg6shp	228	7	was	be	VBD
cord-333636-h2sg6shp	228	8	highly	highly	RB
cord-333636-h2sg6shp	228	9	pure	pure	JJ
cord-333636-h2sg6shp	228	10	and	and	CC
cord-333636-h2sg6shp	228	11	soluble	soluble	JJ
cord-333636-h2sg6shp	228	12	,	,	,
cord-333636-h2sg6shp	228	13	thus	thus	RB
cord-333636-h2sg6shp	228	14	enabling	enable	VBG
cord-333636-h2sg6shp	228	15	us	-PRON-	PRP
cord-333636-h2sg6shp	228	16	to	to	TO
cord-333636-h2sg6shp	228	17	carry	carry	VB
cord-333636-h2sg6shp	228	18	out	out	RP
cord-333636-h2sg6shp	228	19	a	a	DT
cord-333636-h2sg6shp	228	20	number	number	NN
cord-333636-h2sg6shp	228	21	of	of	IN
cord-333636-h2sg6shp	228	22	highly	highly	RB
cord-333636-h2sg6shp	228	23	specific	specific	JJ
cord-333636-h2sg6shp	228	24	assays	assay	NNS
cord-333636-h2sg6shp	228	25	.	.	.
cord-333636-h2sg6shp	229	1	rBV	rBV	NNP
cord-333636-h2sg6shp	229	2	-	-	HYPH
cord-333636-h2sg6shp	229	3	expressed	express	VBN
cord-333636-h2sg6shp	229	4	VP3	VP3	NNP
cord-333636-h2sg6shp	229	5	exhibited	exhibit	VBD
cord-333636-h2sg6shp	229	6	a	a	DT
cord-333636-h2sg6shp	229	7	ssRNA	ssrna	RB
cord-333636-h2sg6shp	229	8	-	-	HYPH
cord-333636-h2sg6shp	229	9	binding	bind	VBG
cord-333636-h2sg6shp	229	10	activity	activity	NN
cord-333636-h2sg6shp	229	11	as	as	IN
cord-333636-h2sg6shp	229	12	determined	determine	VBN
cord-333636-h2sg6shp	229	13	by	by	IN
cord-333636-h2sg6shp	229	14	Northwestern	Northwestern	NNP
cord-333636-h2sg6shp	229	15	and	and	CC
cord-333636-h2sg6shp	229	16	EMSA	EMSA	NNP
cord-333636-h2sg6shp	229	17	assays	assay	NNS
cord-333636-h2sg6shp	229	18	.	.	.
cord-333636-h2sg6shp	230	1	However	however	RB
cord-333636-h2sg6shp	230	2	,	,	,
cord-333636-h2sg6shp	230	3	extracts	extract	NNS
cord-333636-h2sg6shp	230	4	from	from	IN
cord-333636-h2sg6shp	230	5	cells	cell	NNS
cord-333636-h2sg6shp	230	6	infected	infect	VBN
cord-333636-h2sg6shp	230	7	with	with	IN
cord-333636-h2sg6shp	230	8	wildtype	wildtype	NN
cord-333636-h2sg6shp	230	9	baculovirus	baculovirus	NNP
cord-333636-h2sg6shp	230	10	also	also	RB
cord-333636-h2sg6shp	230	11	contained	contain	VBD
cord-333636-h2sg6shp	230	12	an	an	DT
cord-333636-h2sg6shp	230	13	RNA	RNA	NNP
cord-333636-h2sg6shp	230	14	-	-	HYPH
cord-333636-h2sg6shp	230	15	binding	bind	VBG
cord-333636-h2sg6shp	230	16	protein	protein	NN
cord-333636-h2sg6shp	230	17	.	.	.
cord-333636-h2sg6shp	231	1	The	the	DT
cord-333636-h2sg6shp	231	2	presence	presence	NN
cord-333636-h2sg6shp	231	3	of	of	IN
cord-333636-h2sg6shp	231	4	this	this	DT
cord-333636-h2sg6shp	231	5	either	either	DT
cord-333636-h2sg6shp	231	6	cellular	cellular	JJ
cord-333636-h2sg6shp	231	7	or	or	CC
cord-333636-h2sg6shp	231	8	viral	viral	JJ
cord-333636-h2sg6shp	231	9	protein	protein	NN
cord-333636-h2sg6shp	231	10	prevented	prevent	VBD
cord-333636-h2sg6shp	231	11	further	further	JJ
cord-333636-h2sg6shp	231	12	studies	study	NNS
cord-333636-h2sg6shp	231	13	on	on	IN
cord-333636-h2sg6shp	231	14	the	the	DT
cord-333636-h2sg6shp	231	15	VP3	VP3	NNP
cord-333636-h2sg6shp	231	16	RNA	RNA	NNP
cord-333636-h2sg6shp	231	17	-	-	HYPH
cord-333636-h2sg6shp	231	18	binding	bind	VBG
cord-333636-h2sg6shp	231	19	properties	property	NNS
cord-333636-h2sg6shp	231	20	,	,	,
cord-333636-h2sg6shp	231	21	especially	especially	RB
cord-333636-h2sg6shp	231	22	for	for	IN
cord-333636-h2sg6shp	231	23	Kd	Kd	NNP
cord-333636-h2sg6shp	231	24	estimations	estimation	NNS
cord-333636-h2sg6shp	231	25	.	.	.
cord-333636-h2sg6shp	232	1	For	for	IN
cord-333636-h2sg6shp	232	2	this	this	DT
cord-333636-h2sg6shp	232	3	reason	reason	NN
cord-333636-h2sg6shp	232	4	further	further	JJ
cord-333636-h2sg6shp	232	5	studies	study	NNS
cord-333636-h2sg6shp	232	6	were	be	VBD
cord-333636-h2sg6shp	232	7	carried	carry	VBN
cord-333636-h2sg6shp	232	8	out	out	RP
cord-333636-h2sg6shp	232	9	with	with	IN
cord-333636-h2sg6shp	232	10	affinity	affinity	NN
cord-333636-h2sg6shp	232	11	-	-	HYPH
cord-333636-h2sg6shp	232	12	purified	purify	VBN
cord-333636-h2sg6shp	232	13	VP3	vp3	NN
cord-333636-h2sg6shp	232	14	.	.	.
cord-333636-h2sg6shp	233	1	VP3	VP3	NNP
cord-333636-h2sg6shp	233	2	presented	present	VBD
cord-333636-h2sg6shp	233	3	a	a	DT
cord-333636-h2sg6shp	233	4	ssRNA	ssrna	RB
cord-333636-h2sg6shp	233	5	-	-	HYPH
cord-333636-h2sg6shp	233	6	binding	bind	VBG
cord-333636-h2sg6shp	233	7	activity	activity	NN
cord-333636-h2sg6shp	233	8	with	with	IN
cord-333636-h2sg6shp	233	9	an	an	DT
cord-333636-h2sg6shp	233	10	approximate	approximate	JJ
cord-333636-h2sg6shp	233	11	Kd	Kd	NNP
cord-333636-h2sg6shp	233	12	of	of	IN
cord-333636-h2sg6shp	233	13	(	(	-LRB-
cord-333636-h2sg6shp	233	14	2	2	CD
cord-333636-h2sg6shp	233	15	±	±	CD
cord-333636-h2sg6shp	233	16	0.2	0.2	CD
cord-333636-h2sg6shp	233	17	)	)	-RRB-
cord-333636-h2sg6shp	233	18	×	×	NN
cord-333636-h2sg6shp	233	19	10	10	CD
cord-333636-h2sg6shp	234	1	−4	−4	NNP
cord-333636-h2sg6shp	234	2	mM	mm	NN
cord-333636-h2sg6shp	234	3	as	as	RB
cord-333636-h2sg6shp	234	4	directly	directly	RB
cord-333636-h2sg6shp	234	5	determined	determine	VBN
cord-333636-h2sg6shp	234	6	from	from	IN
cord-333636-h2sg6shp	234	7	VP3-RNA	VP3-RNA	NNP
cord-333636-h2sg6shp	234	8	binding	binding	NN
cord-333636-h2sg6shp	234	9	curves	curve	NNS
cord-333636-h2sg6shp	234	10	.	.	.
cord-333636-h2sg6shp	235	1	VP3-RNA	VP3-RNA	NNP
cord-333636-h2sg6shp	235	2	binding	binding	NN
cord-333636-h2sg6shp	235	3	curves	curve	NNS
cord-333636-h2sg6shp	235	4	were	be	VBD
cord-333636-h2sg6shp	235	5	also	also	RB
cord-333636-h2sg6shp	235	6	represented	represent	VBN
cord-333636-h2sg6shp	235	7	as	as	IN
cord-333636-h2sg6shp	235	8	a	a	DT
cord-333636-h2sg6shp	235	9	Hill	Hill	NNP
cord-333636-h2sg6shp	235	10	plot	plot	NN
cord-333636-h2sg6shp	235	11	,	,	,
cord-333636-h2sg6shp	235	12	in	in	IN
cord-333636-h2sg6shp	235	13	order	order	NN
cord-333636-h2sg6shp	235	14	to	to	TO
cord-333636-h2sg6shp	235	15	calculate	calculate	VB
cord-333636-h2sg6shp	235	16	Kd	Kd	NNP
cord-333636-h2sg6shp	235	17	and	and	CC
cord-333636-h2sg6shp	235	18	the	the	DT
cord-333636-h2sg6shp	235	19	Hill	Hill	NNP
cord-333636-h2sg6shp	235	20	coefficient	coefficient	NN
cord-333636-h2sg6shp	235	21	.	.	.
cord-333636-h2sg6shp	236	1	The	the	DT
cord-333636-h2sg6shp	236	2	calculated	calculated	JJ
cord-333636-h2sg6shp	236	3	n	n	NN
cord-333636-h2sg6shp	236	4	H	h	NN
cord-333636-h2sg6shp	236	5	(	(	-LRB-
cord-333636-h2sg6shp	236	6	0.97	0.97	CD
cord-333636-h2sg6shp	236	7	)	)	-RRB-
cord-333636-h2sg6shp	236	8	for	for	IN
cord-333636-h2sg6shp	236	9	VP3-ssRNA	vp3-ssrna	NN
cord-333636-h2sg6shp	236	10	binding	binding	NN
cord-333636-h2sg6shp	236	11	was	be	VBD
cord-333636-h2sg6shp	236	12	slightly	slightly	RB
cord-333636-h2sg6shp	236	13	lower	low	JJR
cord-333636-h2sg6shp	236	14	than	than	IN
cord-333636-h2sg6shp	236	15	1	1	CD
cord-333636-h2sg6shp	236	16	.	.	.
cord-333636-h2sg6shp	237	1	However	however	RB
cord-333636-h2sg6shp	237	2	,	,	,
cord-333636-h2sg6shp	237	3	it	-PRON-	PRP
cord-333636-h2sg6shp	237	4	really	really	RB
cord-333636-h2sg6shp	237	5	can	can	MD
cord-333636-h2sg6shp	237	6	not	not	RB
cord-333636-h2sg6shp	237	7	be	be	VB
cord-333636-h2sg6shp	237	8	considered	consider	VBN
cord-333636-h2sg6shp	237	9	as	as	IN
cord-333636-h2sg6shp	237	10	negative	negative	JJ
cord-333636-h2sg6shp	237	11	cooperativity	cooperativity	NN
cord-333636-h2sg6shp	237	12	,	,	,
cord-333636-h2sg6shp	237	13	but	but	CC
cord-333636-h2sg6shp	237	14	rather	rather	RB
cord-333636-h2sg6shp	237	15	as	as	IN
cord-333636-h2sg6shp	237	16	non	non	JJ
cord-333636-h2sg6shp	237	17	-	-	JJ
cord-333636-h2sg6shp	237	18	cooperative	cooperative	JJ
cord-333636-h2sg6shp	237	19	binding	binding	NN
cord-333636-h2sg6shp	237	20	.	.	.
cord-333636-h2sg6shp	238	1	An	an	DT
cord-333636-h2sg6shp	238	2	alternative	alternative	JJ
cord-333636-h2sg6shp	238	3	derivation	derivation	NN
cord-333636-h2sg6shp	238	4	of	of	IN
cord-333636-h2sg6shp	238	5	Kd	Kd	NNP
cord-333636-h2sg6shp	238	6	by	by	IN
cord-333636-h2sg6shp	238	7	calculating	calculate	VBG
cord-333636-h2sg6shp	238	8	K	K	NNP
cord-333636-h2sg6shp	238	9	0.5	0.5	CD
cord-333636-h2sg6shp	238	10	in	in	IN
cord-333636-h2sg6shp	238	11	the	the	DT
cord-333636-h2sg6shp	238	12	Hill	Hill	NNP
cord-333636-h2sg6shp	238	13	plot	plot	NN
cord-333636-h2sg6shp	238	14	also	also	RB
cord-333636-h2sg6shp	238	15	agreed	agree	VBD
cord-333636-h2sg6shp	238	16	with	with	IN
cord-333636-h2sg6shp	238	17	the	the	DT
cord-333636-h2sg6shp	238	18	estimated	estimate	VBN
cord-333636-h2sg6shp	238	19	Kd	Kd	NNP
cord-333636-h2sg6shp	238	20	for	for	IN
cord-333636-h2sg6shp	238	21	VP3-ssRNA	VP3-ssRNA	NNP
cord-333636-h2sg6shp	238	22	binding	binding	NN
cord-333636-h2sg6shp	238	23	.	.	.
cord-333636-h2sg6shp	239	1	This	this	DT
cord-333636-h2sg6shp	239	2	Kd	Kd	NNP
cord-333636-h2sg6shp	239	3	value	value	NN
cord-333636-h2sg6shp	239	4	falls	fall	VBZ
cord-333636-h2sg6shp	239	5	within	within	IN
cord-333636-h2sg6shp	239	6	the	the	DT
cord-333636-h2sg6shp	239	7	range	range	NN
cord-333636-h2sg6shp	239	8	of	of	IN
cord-333636-h2sg6shp	239	9	other	other	JJ
cord-333636-h2sg6shp	239	10	viral	viral	JJ
cord-333636-h2sg6shp	239	11	and	and	CC
cord-333636-h2sg6shp	239	12	cellular	cellular	JJ
cord-333636-h2sg6shp	239	13	nucleic	nucleic	JJ
cord-333636-h2sg6shp	239	14	acid	acid	NN
cord-333636-h2sg6shp	239	15	-	-	HYPH
cord-333636-h2sg6shp	239	16	binding	bind	VBG
cord-333636-h2sg6shp	239	17	proteins	protein	NNS
cord-333636-h2sg6shp	239	18	[	[	-LRB-
cord-333636-h2sg6shp	239	19	31	31	CD
cord-333636-h2sg6shp	239	20	,	,	,
cord-333636-h2sg6shp	239	21	38	38	CD
cord-333636-h2sg6shp	239	22	,	,	,
cord-333636-h2sg6shp	239	23	42	42	CD
cord-333636-h2sg6shp	239	24	]	]	-RRB-
cord-333636-h2sg6shp	239	25	.	.	.
cord-333636-h2sg6shp	240	1	Interestingly	interestingly	RB
cord-333636-h2sg6shp	240	2	,	,	,
cord-333636-h2sg6shp	240	3	the	the	DT
cord-333636-h2sg6shp	240	4	EMSA	EMSA	NNP
cord-333636-h2sg6shp	240	5	assays	assay	NNS
cord-333636-h2sg6shp	240	6	revealed	reveal	VBD
cord-333636-h2sg6shp	240	7	at	at	IN
cord-333636-h2sg6shp	240	8	least	least	JJS
cord-333636-h2sg6shp	240	9	three	three	CD
cord-333636-h2sg6shp	240	10	VP3-ssRNA	vp3-ssrna	NN
cord-333636-h2sg6shp	240	11	complexes	complex	NNS
cord-333636-h2sg6shp	240	12	with	with	IN
cord-333636-h2sg6shp	240	13	different	different	JJ
cord-333636-h2sg6shp	240	14	electrophoretic	electrophoretic	JJ
cord-333636-h2sg6shp	240	15	mobilities	mobility	NNS
cord-333636-h2sg6shp	240	16	.	.	.
cord-333636-h2sg6shp	241	1	Two	two	CD
cord-333636-h2sg6shp	241	2	possibilities	possibility	NNS
cord-333636-h2sg6shp	241	3	could	could	MD
cord-333636-h2sg6shp	241	4	explain	explain	VB
cord-333636-h2sg6shp	241	5	the	the	DT
cord-333636-h2sg6shp	241	6	presence	presence	NN
cord-333636-h2sg6shp	241	7	of	of	IN
cord-333636-h2sg6shp	241	8	the	the	DT
cord-333636-h2sg6shp	241	9	multiple	multiple	JJ
cord-333636-h2sg6shp	241	10	shifted	shift	VBN
cord-333636-h2sg6shp	241	11	bands	band	NNS
cord-333636-h2sg6shp	241	12	observed	observe	VBD
cord-333636-h2sg6shp	241	13	:	:	:
cord-333636-h2sg6shp	241	14	i	i	LS
cord-333636-h2sg6shp	241	15	)	)	-RRB-
cord-333636-h2sg6shp	241	16	VP3	VP3	NNP
cord-333636-h2sg6shp	241	17	could	could	MD
cord-333636-h2sg6shp	241	18	contain	contain	VB
cord-333636-h2sg6shp	241	19	more	more	JJR
cord-333636-h2sg6shp	241	20	than	than	IN
cord-333636-h2sg6shp	241	21	one	one	CD
cord-333636-h2sg6shp	241	22	RNA	rna	NN
cord-333636-h2sg6shp	241	23	-	-	HYPH
cord-333636-h2sg6shp	241	24	binding	bind	VBG
cord-333636-h2sg6shp	241	25	domain	domain	NN
cord-333636-h2sg6shp	241	26	;	;	:
cord-333636-h2sg6shp	241	27	and	and	CC
cord-333636-h2sg6shp	241	28	ii	ii	LS
cord-333636-h2sg6shp	241	29	)	)	-RRB-
cord-333636-h2sg6shp	241	30	VP3	VP3	NNP
cord-333636-h2sg6shp	241	31	could	could	MD
cord-333636-h2sg6shp	241	32	form	form	VB
cord-333636-h2sg6shp	241	33	oligomers	oligomer	NNS
cord-333636-h2sg6shp	241	34	that	that	WDT
cord-333636-h2sg6shp	241	35	bind	bind	VBP
cord-333636-h2sg6shp	241	36	RNA	RNA	NNP
cord-333636-h2sg6shp	241	37	.	.	.
cord-333636-h2sg6shp	242	1	The	the	DT
cord-333636-h2sg6shp	242	2	most	most	RBS
cord-333636-h2sg6shp	242	3	abundant	abundant	JJ
cord-333636-h2sg6shp	242	4	complex	complex	NN
cord-333636-h2sg6shp	242	5	corresponded	correspond	VBD
cord-333636-h2sg6shp	242	6	to	to	IN
cord-333636-h2sg6shp	242	7	the	the	DT
cord-333636-h2sg6shp	242	8	faster	fast	RBR
cord-333636-h2sg6shp	242	9	migrating	migrating	NN
cord-333636-h2sg6shp	242	10	band	band	NN
cord-333636-h2sg6shp	242	11	,	,	,
cord-333636-h2sg6shp	242	12	even	even	RB
cord-333636-h2sg6shp	242	13	when	when	WRB
cord-333636-h2sg6shp	242	14	extremely	extremely	RB
cord-333636-h2sg6shp	242	15	low	low	JJ
cord-333636-h2sg6shp	242	16	concentrations	concentration	NNS
cord-333636-h2sg6shp	242	17	of	of	IN
cord-333636-h2sg6shp	242	18	VP3	VP3	NNP
cord-333636-h2sg6shp	242	19	were	be	VBD
cord-333636-h2sg6shp	242	20	used	use	VBN
cord-333636-h2sg6shp	242	21	in	in	IN
cord-333636-h2sg6shp	242	22	the	the	DT
cord-333636-h2sg6shp	242	23	assays	assay	NNS
cord-333636-h2sg6shp	242	24	.	.	.
cord-333636-h2sg6shp	243	1	This	this	DT
cord-333636-h2sg6shp	243	2	observation	observation	NN
cord-333636-h2sg6shp	243	3	suggested	suggest	VBD
cord-333636-h2sg6shp	243	4	that	that	IN
cord-333636-h2sg6shp	243	5	most	most	RBS
cord-333636-h2sg6shp	243	6	likely	likely	RB
cord-333636-h2sg6shp	243	7	the	the	DT
cord-333636-h2sg6shp	243	8	faster	fast	RBR
cord-333636-h2sg6shp	243	9	migrating	migrate	VBG
cord-333636-h2sg6shp	243	10	band	band	NN
cord-333636-h2sg6shp	243	11	was	be	VBD
cord-333636-h2sg6shp	243	12	formed	form	VBN
cord-333636-h2sg6shp	243	13	by	by	IN
cord-333636-h2sg6shp	243	14	VP3	VP3	NNP
cord-333636-h2sg6shp	243	15	monomers	monomer	NNS
cord-333636-h2sg6shp	243	16	.	.	.
cord-333636-h2sg6shp	244	1	Moreover	moreover	RB
cord-333636-h2sg6shp	244	2	,	,	,
cord-333636-h2sg6shp	244	3	the	the	DT
cord-333636-h2sg6shp	244	4	binding	binding	NN
cord-333636-h2sg6shp	244	5	of	of	IN
cord-333636-h2sg6shp	244	6	VP3	VP3	NNP
cord-333636-h2sg6shp	244	7	to	to	IN
cord-333636-h2sg6shp	244	8	ssRNA	ssRNA	''
cord-333636-h2sg6shp	244	9	in	in	IN
cord-333636-h2sg6shp	244	10	Northwestern	northwestern	JJ
cord-333636-h2sg6shp	244	11	assays	assay	NNS
cord-333636-h2sg6shp	244	12	showed	show	VBD
cord-333636-h2sg6shp	244	13	that	that	IN
cord-333636-h2sg6shp	244	14	VP3	VP3	NNP
cord-333636-h2sg6shp	244	15	monomers	monomer	NNS
cord-333636-h2sg6shp	244	16	generated	generate	VBN
cord-333636-h2sg6shp	244	17	under	under	IN
cord-333636-h2sg6shp	244	18	denaturing	denaturing	JJ
cord-333636-h2sg6shp	244	19	conditions	condition	NNS
cord-333636-h2sg6shp	244	20	bound	bind	VBN
cord-333636-h2sg6shp	244	21	ssRNA	ssRNA	''
cord-333636-h2sg6shp	244	22	.	.	.
cord-333636-h2sg6shp	245	1	The	the	DT
cord-333636-h2sg6shp	245	2	ability	ability	NN
cord-333636-h2sg6shp	245	3	of	of	IN
cord-333636-h2sg6shp	245	4	VP3	vp3	NN
cord-333636-h2sg6shp	245	5	to	to	TO
cord-333636-h2sg6shp	245	6	form	form	VB
cord-333636-h2sg6shp	245	7	oligomers	oligomer	NNS
cord-333636-h2sg6shp	245	8	has	have	VBZ
cord-333636-h2sg6shp	245	9	been	be	VBN
cord-333636-h2sg6shp	245	10	assessed	assess	VBN
cord-333636-h2sg6shp	245	11	using	use	VBG
cord-333636-h2sg6shp	245	12	a	a	DT
cord-333636-h2sg6shp	245	13	two	two	CD
cord-333636-h2sg6shp	245	14	-	-	HYPH
cord-333636-h2sg6shp	245	15	hybrid	hybrid	NN
cord-333636-h2sg6shp	245	16	system	system	NN
cord-333636-h2sg6shp	245	17	approach	approach	NN
cord-333636-h2sg6shp	245	18	[	[	-LRB-
cord-333636-h2sg6shp	245	19	43	43	CD
cord-333636-h2sg6shp	245	20	]	]	-RRB-
cord-333636-h2sg6shp	245	21	,	,	,
cord-333636-h2sg6shp	245	22	and	and	CC
cord-333636-h2sg6shp	245	23	the	the	DT
cord-333636-h2sg6shp	245	24	structural	structural	JJ
cord-333636-h2sg6shp	245	25	analyses	analysis	NNS
cord-333636-h2sg6shp	245	26	of	of	IN
cord-333636-h2sg6shp	245	27	IBDV	IBDV	NNP
cord-333636-h2sg6shp	245	28	particles	particle	NNS
cord-333636-h2sg6shp	245	29	and	and	CC
cord-333636-h2sg6shp	245	30	VLPs	vlp	NNS
cord-333636-h2sg6shp	245	31	have	have	VBP
cord-333636-h2sg6shp	245	32	revealed	reveal	VBN
cord-333636-h2sg6shp	245	33	that	that	IN
cord-333636-h2sg6shp	245	34	VP3	vp3	JJ
cord-333636-h2sg6shp	245	35	molecules	molecule	NNS
cord-333636-h2sg6shp	245	36	form	form	VBP
cord-333636-h2sg6shp	245	37	trimers	trimer	NNS
cord-333636-h2sg6shp	245	38	in	in	IN
cord-333636-h2sg6shp	245	39	the	the	DT
cord-333636-h2sg6shp	245	40	internal	internal	JJ
cord-333636-h2sg6shp	245	41	surface	surface	NN
cord-333636-h2sg6shp	245	42	of	of	IN
cord-333636-h2sg6shp	245	43	the	the	DT
cord-333636-h2sg6shp	245	44	capsid	capsid	NN
cord-333636-h2sg6shp	245	45	[	[	-LRB-
cord-333636-h2sg6shp	245	46	9	9	CD
cord-333636-h2sg6shp	245	47	]	]	-RRB-
cord-333636-h2sg6shp	245	48	.	.	.
cord-333636-h2sg6shp	246	1	Therefore	therefore	RB
cord-333636-h2sg6shp	246	2	,	,	,
cord-333636-h2sg6shp	246	3	it	-PRON-	PRP
cord-333636-h2sg6shp	246	4	is	be	VBZ
cord-333636-h2sg6shp	246	5	likely	likely	JJ
cord-333636-h2sg6shp	246	6	that	that	IN
cord-333636-h2sg6shp	246	7	VP3	VP3	NNP
cord-333636-h2sg6shp	246	8	might	may	MD
cord-333636-h2sg6shp	246	9	also	also	RB
cord-333636-h2sg6shp	246	10	form	form	VB
cord-333636-h2sg6shp	246	11	trimers	trimer	NNS
cord-333636-h2sg6shp	246	12	under	under	IN
cord-333636-h2sg6shp	246	13	the	the	DT
cord-333636-h2sg6shp	246	14	conditions	condition	NNS
cord-333636-h2sg6shp	246	15	used	use	VBN
cord-333636-h2sg6shp	246	16	for	for	IN
cord-333636-h2sg6shp	246	17	RNA	rna	NN
cord-333636-h2sg6shp	246	18	-	-	HYPH
cord-333636-h2sg6shp	246	19	binding	bind	VBG
cord-333636-h2sg6shp	246	20	studies	study	NNS
cord-333636-h2sg6shp	246	21	,	,	,
cord-333636-h2sg6shp	246	22	explaining	explain	VBG
cord-333636-h2sg6shp	246	23	the	the	DT
cord-333636-h2sg6shp	246	24	presence	presence	NN
cord-333636-h2sg6shp	246	25	of	of	IN
cord-333636-h2sg6shp	246	26	lower	low	JJR
cord-333636-h2sg6shp	246	27	mobility	mobility	NN
cord-333636-h2sg6shp	246	28	shift	shift	NN
cord-333636-h2sg6shp	246	29	bands	band	NNS
cord-333636-h2sg6shp	246	30	when	when	WRB
cord-333636-h2sg6shp	246	31	VP3	VP3	NNP
cord-333636-h2sg6shp	246	32	concentration	concentration	NN
cord-333636-h2sg6shp	246	33	was	be	VBD
cord-333636-h2sg6shp	246	34	increased	increase	VBN
cord-333636-h2sg6shp	246	35	.	.	.
cord-333636-h2sg6shp	247	1	However	however	RB
cord-333636-h2sg6shp	247	2	,	,	,
cord-333636-h2sg6shp	247	3	the	the	DT
cord-333636-h2sg6shp	247	4	ability	ability	NN
cord-333636-h2sg6shp	247	5	of	of	IN
cord-333636-h2sg6shp	247	6	recombinant	recombinant	JJ
cord-333636-h2sg6shp	247	7	VP3	vp3	NN
cord-333636-h2sg6shp	247	8	to	to	TO
cord-333636-h2sg6shp	247	9	form	form	VB
cord-333636-h2sg6shp	247	10	trimers	trimer	NNS
cord-333636-h2sg6shp	247	11	has	have	VBZ
cord-333636-h2sg6shp	247	12	not	not	RB
cord-333636-h2sg6shp	247	13	been	be	VBN
cord-333636-h2sg6shp	247	14	tested	test	VBN
cord-333636-h2sg6shp	247	15	in	in	IN
cord-333636-h2sg6shp	247	16	this	this	DT
cord-333636-h2sg6shp	247	17	work	work	NN
cord-333636-h2sg6shp	247	18	.	.	.
cord-333636-h2sg6shp	248	1	Competition	competition	NN
cord-333636-h2sg6shp	248	2	experiments	experiment	NNS
cord-333636-h2sg6shp	248	3	revealed	reveal	VBD
cord-333636-h2sg6shp	248	4	that	that	IN
cord-333636-h2sg6shp	248	5	VP3	VP3	NNP
cord-333636-h2sg6shp	248	6	bound	bind	VBN
cord-333636-h2sg6shp	248	7	ssRNA	ssrna	RB
cord-333636-h2sg6shp	248	8	in	in	IN
cord-333636-h2sg6shp	248	9	a	a	DT
cord-333636-h2sg6shp	248	10	sequenceindependent	sequenceindependent	NN
cord-333636-h2sg6shp	248	11	manner	manner	NN
cord-333636-h2sg6shp	248	12	.	.	.
cord-333636-h2sg6shp	249	1	The	the	DT
cord-333636-h2sg6shp	249	2	lack	lack	NN
cord-333636-h2sg6shp	249	3	of	of	IN
cord-333636-h2sg6shp	249	4	any	any	DT
cord-333636-h2sg6shp	249	5	binding	bind	VBG
cord-333636-h2sg6shp	249	6	specificity	specificity	NN
cord-333636-h2sg6shp	249	7	to	to	IN
cord-333636-h2sg6shp	249	8	ssRNA	ssRNA	VBG
cord-333636-h2sg6shp	249	9	probes	probe	NNS
cord-333636-h2sg6shp	249	10	used	use	VBN
cord-333636-h2sg6shp	249	11	in	in	IN
cord-333636-h2sg6shp	249	12	this	this	DT
cord-333636-h2sg6shp	249	13	study	study	NN
cord-333636-h2sg6shp	249	14	might	may	MD
cord-333636-h2sg6shp	249	15	indicate	indicate	VB
cord-333636-h2sg6shp	249	16	that	that	DT
cord-333636-h2sg6shp	249	17	,	,	,
cord-333636-h2sg6shp	249	18	as	as	IN
cord-333636-h2sg6shp	249	19	it	-PRON-	PRP
cord-333636-h2sg6shp	249	20	is	be	VBZ
cord-333636-h2sg6shp	249	21	the	the	DT
cord-333636-h2sg6shp	249	22	case	case	NN
cord-333636-h2sg6shp	249	23	with	with	IN
cord-333636-h2sg6shp	249	24	other	other	JJ
cord-333636-h2sg6shp	249	25	virus	virus	NN
cord-333636-h2sg6shp	249	26	-	-	HYPH
cord-333636-h2sg6shp	249	27	encoded	encode	VBN
cord-333636-h2sg6shp	249	28	nucleoproteins	nucleoprotein	NNS
cord-333636-h2sg6shp	249	29	[	[	-LRB-
cord-333636-h2sg6shp	249	30	20	20	CD
cord-333636-h2sg6shp	249	31	,	,	,
cord-333636-h2sg6shp	249	32	26	26	CD
cord-333636-h2sg6shp	249	33	,	,	,
cord-333636-h2sg6shp	249	34	33	33	CD
cord-333636-h2sg6shp	249	35	,	,	,
cord-333636-h2sg6shp	249	36	42	42	CD
cord-333636-h2sg6shp	249	37	]	]	-RRB-
cord-333636-h2sg6shp	249	38	,	,	,
cord-333636-h2sg6shp	249	39	other	other	JJ
cord-333636-h2sg6shp	249	40	factor(s	factor(s	NNP
cord-333636-h2sg6shp	249	41	)	)	-RRB-
cord-333636-h2sg6shp	249	42	could	could	MD
cord-333636-h2sg6shp	249	43	be	be	VB
cord-333636-h2sg6shp	249	44	necessary	necessary	JJ
cord-333636-h2sg6shp	249	45	to	to	TO
cord-333636-h2sg6shp	249	46	confer	confer	VB
cord-333636-h2sg6shp	249	47	specificity	specificity	NN
cord-333636-h2sg6shp	249	48	.	.	.
cord-333636-h2sg6shp	250	1	VP1	VP1	NNP
cord-333636-h2sg6shp	250	2	,	,	,
cord-333636-h2sg6shp	250	3	the	the	DT
cord-333636-h2sg6shp	250	4	putative	putative	JJ
cord-333636-h2sg6shp	250	5	IBDV	IBDV	NNP
cord-333636-h2sg6shp	250	6	RdRp	RdRp	NNP
cord-333636-h2sg6shp	250	7	,	,	,
cord-333636-h2sg6shp	250	8	is	be	VBZ
cord-333636-h2sg6shp	250	9	the	the	DT
cord-333636-h2sg6shp	250	10	most	most	RBS
cord-333636-h2sg6shp	250	11	likely	likely	JJ
cord-333636-h2sg6shp	250	12	candidate	candidate	NN
cord-333636-h2sg6shp	250	13	for	for	IN
cord-333636-h2sg6shp	250	14	this	this	DT
cord-333636-h2sg6shp	250	15	role	role	NN
cord-333636-h2sg6shp	250	16	.	.	.
cord-333636-h2sg6shp	251	1	Interestingly	interestingly	RB
cord-333636-h2sg6shp	251	2	,	,	,
cord-333636-h2sg6shp	251	3	VP3	VP3	NNP
cord-333636-h2sg6shp	251	4	bound	bind	VBN
cord-333636-h2sg6shp	251	5	dsRNA	dsrna	NN
cord-333636-h2sg6shp	251	6	and	and	CC
cord-333636-h2sg6shp	251	7	ssDNA	ssdna	RB
cord-333636-h2sg6shp	251	8	,	,	,
cord-333636-h2sg6shp	251	9	apparently	apparently	RB
cord-333636-h2sg6shp	251	10	through	through	IN
cord-333636-h2sg6shp	251	11	the	the	DT
cord-333636-h2sg6shp	251	12	same	same	JJ
cord-333636-h2sg6shp	251	13	ssRNA	ssrna	NN
cord-333636-h2sg6shp	251	14	-	-	HYPH
cord-333636-h2sg6shp	251	15	binding	bind	VBG
cord-333636-h2sg6shp	251	16	domain	domain	NN
cord-333636-h2sg6shp	251	17	,	,	,
cord-333636-h2sg6shp	251	18	but	but	CC
cord-333636-h2sg6shp	251	19	not	not	RB
cord-333636-h2sg6shp	251	20	dsDNA	dsdna	RB
cord-333636-h2sg6shp	251	21	.	.	.
cord-333636-h2sg6shp	252	1	These	these	DT
cord-333636-h2sg6shp	252	2	binding	bind	VBG
cord-333636-h2sg6shp	252	3	characteristics	characteristic	NNS
cord-333636-h2sg6shp	252	4	are	be	VBP
cord-333636-h2sg6shp	252	5	usually	usually	RB
cord-333636-h2sg6shp	252	6	found	find	VBN
cord-333636-h2sg6shp	252	7	in	in	IN
cord-333636-h2sg6shp	252	8	RNA	rna	NN
cord-333636-h2sg6shp	252	9	-	-	HYPH
cord-333636-h2sg6shp	252	10	binding	bind	VBG
cord-333636-h2sg6shp	252	11	proteins	protein	NNS
cord-333636-h2sg6shp	252	12	,	,	,
cord-333636-h2sg6shp	252	13	especially	especially	RB
cord-333636-h2sg6shp	252	14	in	in	IN
cord-333636-h2sg6shp	252	15	dsRNA	dsrna	JJ
cord-333636-h2sg6shp	252	16	-	-	HYPH
cord-333636-h2sg6shp	252	17	binding	bind	VBG
cord-333636-h2sg6shp	252	18	proteins	protein	NNS
cord-333636-h2sg6shp	252	19	[	[	-LRB-
cord-333636-h2sg6shp	252	20	14	14	CD
cord-333636-h2sg6shp	252	21	]	]	-RRB-
cord-333636-h2sg6shp	252	22	.	.	.
cord-333636-h2sg6shp	253	1	The	the	DT
cord-333636-h2sg6shp	253	2	ability	ability	NN
cord-333636-h2sg6shp	253	3	of	of	IN
cord-333636-h2sg6shp	253	4	VP3	VP3	NNP
cord-333636-h2sg6shp	253	5	to	to	TO
cord-333636-h2sg6shp	253	6	interact	interact	VB
cord-333636-h2sg6shp	253	7	with	with	IN
cord-333636-h2sg6shp	253	8	ssRNA	ssRNA	.
cord-333636-h2sg6shp	254	1	and	and	CC
cord-333636-h2sg6shp	254	2	dsRNA	dsrna	NN
cord-333636-h2sg6shp	254	3	could	could	MD
cord-333636-h2sg6shp	254	4	reflect	reflect	VB
cord-333636-h2sg6shp	254	5	the	the	DT
cord-333636-h2sg6shp	254	6	possibility	possibility	NN
cord-333636-h2sg6shp	254	7	that	that	IN
cord-333636-h2sg6shp	254	8	genome	genome	NN
cord-333636-h2sg6shp	254	9	replication	replication	NN
cord-333636-h2sg6shp	254	10	and	and	CC
cord-333636-h2sg6shp	254	11	transcription	transcription	NN
cord-333636-h2sg6shp	254	12	,	,	,
cord-333636-h2sg6shp	254	13	assumed	assume	VBD
cord-333636-h2sg6shp	254	14	to	to	TO
cord-333636-h2sg6shp	254	15	be	be	VB
cord-333636-h2sg6shp	254	16	carried	carry	VBN
cord-333636-h2sg6shp	254	17	out	out	RP
cord-333636-h2sg6shp	254	18	by	by	IN
cord-333636-h2sg6shp	254	19	VP1	VP1	NNP
cord-333636-h2sg6shp	254	20	,	,	,
cord-333636-h2sg6shp	254	21	could	could	MD
cord-333636-h2sg6shp	254	22	be	be	VB
cord-333636-h2sg6shp	254	23	assisted	assist	VBN
cord-333636-h2sg6shp	254	24	by	by	IN
cord-333636-h2sg6shp	254	25	VP3	VP3	NNP
cord-333636-h2sg6shp	254	26	binding	bind	VBG
cord-333636-h2sg6shp	254	27	both	both	DT
cord-333636-h2sg6shp	254	28	to	to	IN
cord-333636-h2sg6shp	254	29	IBDV	IBDV	NNP
cord-333636-h2sg6shp	254	30	ssRNA	ssRNA	''
cord-333636-h2sg6shp	255	1	and	and	CC
cord-333636-h2sg6shp	255	2	dsRNA	dsrna	NN
cord-333636-h2sg6shp	255	3	.	.	.
cord-333636-h2sg6shp	256	1	In	in	IN
cord-333636-h2sg6shp	256	2	order	order	NN
cord-333636-h2sg6shp	256	3	to	to	TO
cord-333636-h2sg6shp	256	4	identify	identify	VB
cord-333636-h2sg6shp	256	5	the	the	DT
cord-333636-h2sg6shp	256	6	VP3	VP3	NNP
cord-333636-h2sg6shp	256	7	protein	protein	NN
cord-333636-h2sg6shp	256	8	domain	domain	NN
cord-333636-h2sg6shp	256	9	involved	involve	VBN
cord-333636-h2sg6shp	256	10	in	in	IN
cord-333636-h2sg6shp	256	11	RNA	RNA	NNP
cord-333636-h2sg6shp	256	12	-	-	HYPH
cord-333636-h2sg6shp	256	13	binding	binding	NN
cord-333636-h2sg6shp	256	14	,	,	,
cord-333636-h2sg6shp	256	15	a	a	DT
cord-333636-h2sg6shp	256	16	series	series	NN
cord-333636-h2sg6shp	256	17	of	of	IN
cord-333636-h2sg6shp	256	18	deletion	deletion	NN
cord-333636-h2sg6shp	256	19	mutants	mutant	NNS
cord-333636-h2sg6shp	256	20	were	be	VBD
cord-333636-h2sg6shp	256	21	expressed	express	VBN
cord-333636-h2sg6shp	256	22	and	and	CC
cord-333636-h2sg6shp	256	23	purified	purify	VBN
cord-333636-h2sg6shp	256	24	.	.	.
cord-333636-h2sg6shp	257	1	Interestingly	interestingly	RB
cord-333636-h2sg6shp	257	2	,	,	,
cord-333636-h2sg6shp	257	3	two	two	CD
cord-333636-h2sg6shp	257	4	protein	protein	NN
cord-333636-h2sg6shp	257	5	forms	form	NNS
cord-333636-h2sg6shp	257	6	were	be	VBD
cord-333636-h2sg6shp	257	7	observed	observe	VBN
cord-333636-h2sg6shp	257	8	for	for	IN
cord-333636-h2sg6shp	257	9	each	each	DT
cord-333636-h2sg6shp	257	10	mutant	mutant	JJ
cord-333636-h2sg6shp	257	11	,	,	,
cord-333636-h2sg6shp	257	12	except	except	IN
cord-333636-h2sg6shp	257	13	for	for	IN
cord-333636-h2sg6shp	257	14	VP3	VP3	NNP
cord-333636-h2sg6shp	257	15	971	971	CD
cord-333636-h2sg6shp	257	16	-	-	HYPH
cord-333636-h2sg6shp	257	17	1012	1012	CD
cord-333636-h2sg6shp	257	18	.	.	.
cord-333636-h2sg6shp	258	1	This	this	DT
cord-333636-h2sg6shp	258	2	is	be	VBZ
cord-333636-h2sg6shp	258	3	consistent	consistent	JJ
cord-333636-h2sg6shp	258	4	with	with	IN
cord-333636-h2sg6shp	258	5	a	a	DT
cord-333636-h2sg6shp	258	6	proteolytic	proteolytic	JJ
cord-333636-h2sg6shp	258	7	processing	processing	NN
cord-333636-h2sg6shp	258	8	at	at	IN
cord-333636-h2sg6shp	258	9	the	the	DT
cord-333636-h2sg6shp	258	10	VP3	VP3	NNP
cord-333636-h2sg6shp	258	11	carboxy	carboxy	NNP
cord-333636-h2sg6shp	258	12	terminus	terminus	NN
cord-333636-h2sg6shp	258	13	,	,	,
cord-333636-h2sg6shp	258	14	since	since	IN
cord-333636-h2sg6shp	258	15	VP3	VP3	NNP
cord-333636-h2sg6shp	258	16	971	971	CD
cord-333636-h2sg6shp	258	17	-	-	HYPH
cord-333636-h2sg6shp	258	18	1012	1012	CD
cord-333636-h2sg6shp	258	19	deletion	deletion	NN
cord-333636-h2sg6shp	258	20	mutant	mutant	NN
cord-333636-h2sg6shp	258	21	lacking	lack	VBG
cord-333636-h2sg6shp	258	22	the	the	DT
cord-333636-h2sg6shp	258	23	41	41	CD
cord-333636-h2sg6shp	258	24	C	c	NN
cord-333636-h2sg6shp	258	25	-	-	HYPH
cord-333636-h2sg6shp	258	26	terminal	terminal	NN
cord-333636-h2sg6shp	258	27	residues	residue	NNS
cord-333636-h2sg6shp	258	28	only	only	RB
cord-333636-h2sg6shp	258	29	presented	present	VBD
cord-333636-h2sg6shp	258	30	a	a	DT
cord-333636-h2sg6shp	258	31	single	single	JJ
cord-333636-h2sg6shp	258	32	band	band	NN
cord-333636-h2sg6shp	258	33	of	of	IN
cord-333636-h2sg6shp	258	34	the	the	DT
cord-333636-h2sg6shp	258	35	expected	expect	VBN
cord-333636-h2sg6shp	258	36	molecular	molecular	JJ
cord-333636-h2sg6shp	258	37	mass	mass	NN
cord-333636-h2sg6shp	258	38	(	(	-LRB-
cord-333636-h2sg6shp	258	39	Fig	Fig	NNP
cord-333636-h2sg6shp	258	40	.	.	.
cord-333636-h2sg6shp	258	41	6	6	CD
cord-333636-h2sg6shp	258	42	)	)	-RRB-
cord-333636-h2sg6shp	258	43	.	.	.
cord-333636-h2sg6shp	259	1	VP3	VP3	NNP
cord-333636-h2sg6shp	259	2	presents	present	VBZ
cord-333636-h2sg6shp	259	3	two	two	CD
cord-333636-h2sg6shp	259	4	electrophoretic	electrophoretic	JJ
cord-333636-h2sg6shp	259	5	forms	form	NNS
cord-333636-h2sg6shp	259	6	in	in	IN
cord-333636-h2sg6shp	259	7	IBDV	IBDV	NNP
cord-333636-h2sg6shp	259	8	-	-	HYPH
cord-333636-h2sg6shp	259	9	infected	infect	VBN
cord-333636-h2sg6shp	259	10	cells	cell	NNS
cord-333636-h2sg6shp	259	11	.	.	.
cord-333636-h2sg6shp	260	1	VP3	VP3	NNP
cord-333636-h2sg6shp	260	2	doublets	doublet	NNS
cord-333636-h2sg6shp	260	3	have	have	VBP
cord-333636-h2sg6shp	260	4	been	be	VBN
cord-333636-h2sg6shp	260	5	described	describe	VBN
cord-333636-h2sg6shp	260	6	in	in	IN
cord-333636-h2sg6shp	260	7	previous	previous	JJ
cord-333636-h2sg6shp	260	8	reports	report	NNS
cord-333636-h2sg6shp	260	9	.	.	.
cord-333636-h2sg6shp	261	1	However	however	RB
cord-333636-h2sg6shp	261	2	the	the	DT
cord-333636-h2sg6shp	261	3	nature	nature	NN
cord-333636-h2sg6shp	261	4	and	and	CC
cord-333636-h2sg6shp	261	5	function	function	NN
cord-333636-h2sg6shp	261	6	of	of	IN
cord-333636-h2sg6shp	261	7	these	these	DT
cord-333636-h2sg6shp	261	8	forms	form	NNS
cord-333636-h2sg6shp	261	9	remain	remain	VBP
cord-333636-h2sg6shp	261	10	unknown	unknown	JJ
cord-333636-h2sg6shp	261	11	[	[	-LRB-
cord-333636-h2sg6shp	261	12	43	43	CD
cord-333636-h2sg6shp	261	13	]	]	-RRB-
cord-333636-h2sg6shp	261	14	.	.	.
cord-333636-h2sg6shp	262	1	Interestingly	interestingly	RB
cord-333636-h2sg6shp	262	2	,	,	,
cord-333636-h2sg6shp	262	3	only	only	RB
cord-333636-h2sg6shp	262	4	the	the	DT
cord-333636-h2sg6shp	262	5	full	full	JJ
cord-333636-h2sg6shp	262	6	length	length	NN
cord-333636-h2sg6shp	262	7	VP3	vp3	NN
cord-333636-h2sg6shp	262	8	protein	protein	NN
cord-333636-h2sg6shp	262	9	is	be	VBZ
cord-333636-h2sg6shp	262	10	incorporated	incorporate	VBN
cord-333636-h2sg6shp	262	11	in	in	IN
cord-333636-h2sg6shp	262	12	IBDV	IBDV	NNP
cord-333636-h2sg6shp	262	13	virions	virion	NNS
cord-333636-h2sg6shp	262	14	,	,	,
cord-333636-h2sg6shp	262	15	suggesting	suggest	VBG
cord-333636-h2sg6shp	262	16	that	that	IN
cord-333636-h2sg6shp	262	17	the	the	DT
cord-333636-h2sg6shp	262	18	shorter	short	JJR
cord-333636-h2sg6shp	262	19	form	form	NN
cord-333636-h2sg6shp	262	20	might	may	MD
cord-333636-h2sg6shp	262	21	have	have	VB
cord-333636-h2sg6shp	262	22	additional	additional	JJ
cord-333636-h2sg6shp	262	23	,	,	,
cord-333636-h2sg6shp	262	24	non	non	JJ
cord-333636-h2sg6shp	262	25	-	-	JJ
cord-333636-h2sg6shp	262	26	structural	structural	JJ
cord-333636-h2sg6shp	262	27	,	,	,
cord-333636-h2sg6shp	262	28	functions	function	NNS
cord-333636-h2sg6shp	262	29	.	.	.
cord-333636-h2sg6shp	263	1	It	-PRON-	PRP
cord-333636-h2sg6shp	263	2	could	could	MD
cord-333636-h2sg6shp	263	3	also	also	RB
cord-333636-h2sg6shp	263	4	be	be	VB
cord-333636-h2sg6shp	263	5	possible	possible	JJ
cord-333636-h2sg6shp	263	6	that	that	IN
cord-333636-h2sg6shp	263	7	cellular	cellular	JJ
cord-333636-h2sg6shp	263	8	proteases	protease	NNS
cord-333636-h2sg6shp	263	9	such	such	JJ
cord-333636-h2sg6shp	263	10	as	as	IN
cord-333636-h2sg6shp	263	11	caspases	caspase	NNS
cord-333636-h2sg6shp	263	12	[	[	-LRB-
cord-333636-h2sg6shp	263	13	15	15	CD
cord-333636-h2sg6shp	263	14	]	]	-RRB-
cord-333636-h2sg6shp	263	15	could	could	MD
cord-333636-h2sg6shp	263	16	be	be	VB
cord-333636-h2sg6shp	263	17	involved	involve	VBN
cord-333636-h2sg6shp	263	18	in	in	IN
cord-333636-h2sg6shp	263	19	VP3	vp3	JJ
cord-333636-h2sg6shp	263	20	processing	processing	NN
cord-333636-h2sg6shp	263	21	.	.	.
cord-333636-h2sg6shp	264	1	Alternatively	alternatively	RB
cord-333636-h2sg6shp	264	2	,	,	,
cord-333636-h2sg6shp	264	3	VP3	VP3	NNP
cord-333636-h2sg6shp	264	4	could	could	MD
cord-333636-h2sg6shp	264	5	exhibit	exhibit	VB
cord-333636-h2sg6shp	264	6	an	an	DT
cord-333636-h2sg6shp	264	7	autoproteolytic	autoproteolytic	JJ
cord-333636-h2sg6shp	264	8	activity	activity	NN
cord-333636-h2sg6shp	264	9	not	not	RB
cord-333636-h2sg6shp	264	10	previously	previously	RB
cord-333636-h2sg6shp	264	11	reported	report	VBN
cord-333636-h2sg6shp	264	12	.	.	.
cord-333636-h2sg6shp	265	1	The	the	DT
cord-333636-h2sg6shp	265	2	Kd	Kd	NNP
cord-333636-h2sg6shp	265	3	for	for	IN
cord-333636-h2sg6shp	265	4	each	each	DT
cord-333636-h2sg6shp	265	5	VP3	VP3	NNP
cord-333636-h2sg6shp	265	6	deletion	deletion	NN
cord-333636-h2sg6shp	265	7	mutant	mutant	NN
cord-333636-h2sg6shp	265	8	was	be	VBD
cord-333636-h2sg6shp	265	9	determined	determine	VBN
cord-333636-h2sg6shp	265	10	,	,	,
cord-333636-h2sg6shp	265	11	and	and	CC
cord-333636-h2sg6shp	265	12	it	-PRON-	PRP
cord-333636-h2sg6shp	265	13	was	be	VBD
cord-333636-h2sg6shp	265	14	found	find	VBN
cord-333636-h2sg6shp	265	15	that	that	IN
cord-333636-h2sg6shp	265	16	deletions	deletion	NNS
cord-333636-h2sg6shp	265	17	affecting	affect	VBG
cord-333636-h2sg6shp	265	18	to	to	IN
cord-333636-h2sg6shp	265	19	the	the	DT
cord-333636-h2sg6shp	265	20	VP3	vp3	JJ
cord-333636-h2sg6shp	265	21	region	region	NN
cord-333636-h2sg6shp	265	22	between	between	IN
cord-333636-h2sg6shp	265	23	residues	residue	NNS
cord-333636-h2sg6shp	265	24	808	808	CD
cord-333636-h2sg6shp	265	25	to	to	IN
cord-333636-h2sg6shp	265	26	879	879	CD
cord-333636-h2sg6shp	265	27	abrogated	abrogated	JJ
cord-333636-h2sg6shp	265	28	VP3	VP3	NNP
cord-333636-h2sg6shp	265	29	ssRNA	ssrna	RB
cord-333636-h2sg6shp	265	30	-	-	HYPH
cord-333636-h2sg6shp	265	31	binding	bind	VBG
cord-333636-h2sg6shp	265	32	activity	activity	NN
cord-333636-h2sg6shp	265	33	.	.	.
cord-333636-h2sg6shp	266	1	The	the	DT
cord-333636-h2sg6shp	266	2	loss	loss	NN
cord-333636-h2sg6shp	266	3	of	of	IN
cord-333636-h2sg6shp	266	4	ssRNA	ssrna	RB
cord-333636-h2sg6shp	266	5	-	-	HYPH
cord-333636-h2sg6shp	266	6	binding	bind	VBG
cord-333636-h2sg6shp	266	7	activity	activity	NN
cord-333636-h2sg6shp	266	8	exhibited	exhibit	VBN
cord-333636-h2sg6shp	266	9	by	by	IN
cord-333636-h2sg6shp	266	10	these	these	DT
cord-333636-h2sg6shp	266	11	VP3	VP3	NNP
cord-333636-h2sg6shp	266	12	deletion	deletion	NN
cord-333636-h2sg6shp	266	13	mutants	mutant	NNS
cord-333636-h2sg6shp	266	14	was	be	VBD
cord-333636-h2sg6shp	266	15	not	not	RB
cord-333636-h2sg6shp	266	16	due	due	JJ
cord-333636-h2sg6shp	266	17	to	to	IN
cord-333636-h2sg6shp	266	18	loss	loss	NN
cord-333636-h2sg6shp	266	19	of	of	IN
cord-333636-h2sg6shp	266	20	protein	protein	NN
cord-333636-h2sg6shp	266	21	solubility	solubility	NN
cord-333636-h2sg6shp	266	22	,	,	,
cord-333636-h2sg6shp	266	23	since	since	IN
cord-333636-h2sg6shp	266	24	in	in	IN
cord-333636-h2sg6shp	266	25	all	all	DT
cord-333636-h2sg6shp	266	26	experiments	experiment	NNS
cord-333636-h2sg6shp	266	27	VP3	VP3	NNP
cord-333636-h2sg6shp	266	28	mutants	mutant	NNS
cord-333636-h2sg6shp	266	29	were	be	VBD
cord-333636-h2sg6shp	266	30	soluble	soluble	JJ
cord-333636-h2sg6shp	266	31	.	.	.
cord-333636-h2sg6shp	267	1	Although	although	IN
cord-333636-h2sg6shp	267	2	deletions	deletion	NNS
cord-333636-h2sg6shp	267	3	could	could	MD
cord-333636-h2sg6shp	267	4	affect	affect	VB
cord-333636-h2sg6shp	267	5	the	the	DT
cord-333636-h2sg6shp	267	6	VP3	vp3	JJ
cord-333636-h2sg6shp	267	7	structure	structure	NN
cord-333636-h2sg6shp	267	8	,	,	,
cord-333636-h2sg6shp	267	9	this	this	DT
cord-333636-h2sg6shp	267	10	is	be	VBZ
cord-333636-h2sg6shp	267	11	unlikely	unlikely	JJ
cord-333636-h2sg6shp	267	12	.	.	.
cord-333636-h2sg6shp	268	1	Small	small	JJ
cord-333636-h2sg6shp	268	2	deletions	deletion	NNS
cord-333636-h2sg6shp	268	3	were	be	VBD
cord-333636-h2sg6shp	268	4	introduced	introduce	VBN
cord-333636-h2sg6shp	268	5	to	to	TO
cord-333636-h2sg6shp	268	6	avoid	avoid	VB
cord-333636-h2sg6shp	268	7	dramatic	dramatic	JJ
cord-333636-h2sg6shp	268	8	changes	change	NNS
cord-333636-h2sg6shp	268	9	in	in	IN
cord-333636-h2sg6shp	268	10	protein	protein	NN
cord-333636-h2sg6shp	268	11	structure	structure	NN
cord-333636-h2sg6shp	268	12	.	.	.
cord-333636-h2sg6shp	269	1	Additionally	additionally	RB
cord-333636-h2sg6shp	269	2	,	,	,
cord-333636-h2sg6shp	269	3	only	only	RB
cord-333636-h2sg6shp	269	4	deletions	deletion	NNS
cord-333636-h2sg6shp	269	5	between	between	IN
cord-333636-h2sg6shp	269	6	residues	residue	NNS
cord-333636-h2sg6shp	269	7	808	808	CD
cord-333636-h2sg6shp	269	8	and	and	CC
cord-333636-h2sg6shp	269	9	879	879	CD
cord-333636-h2sg6shp	269	10	did	do	VBD
cord-333636-h2sg6shp	269	11	have	have	VB
cord-333636-h2sg6shp	269	12	an	an	DT
cord-333636-h2sg6shp	269	13	effect	effect	NN
cord-333636-h2sg6shp	269	14	on	on	IN
cord-333636-h2sg6shp	269	15	ssRNA	ssrna	RB
cord-333636-h2sg6shp	269	16	-	-	HYPH
cord-333636-h2sg6shp	269	17	binding	bind	VBG
cord-333636-h2sg6shp	269	18	activity	activity	NN
cord-333636-h2sg6shp	269	19	,	,	,
cord-333636-h2sg6shp	269	20	while	while	IN
cord-333636-h2sg6shp	269	21	the	the	DT
cord-333636-h2sg6shp	269	22	rest	rest	NN
cord-333636-h2sg6shp	269	23	of	of	IN
cord-333636-h2sg6shp	269	24	the	the	DT
cord-333636-h2sg6shp	269	25	mutants	mutant	NNS
cord-333636-h2sg6shp	269	26	efficiently	efficiently	RB
cord-333636-h2sg6shp	269	27	bound	bind	VBN
cord-333636-h2sg6shp	269	28	ssRNA	ssRNA	''
cord-333636-h2sg6shp	269	29	.	.	.
cord-333636-h2sg6shp	270	1	Interestingly	interestingly	RB
cord-333636-h2sg6shp	270	2	,	,	,
cord-333636-h2sg6shp	270	3	ssRNA	ssRNA	VBG
cord-333636-h2sg6shp	270	4	-	-	HYPH
cord-333636-h2sg6shp	270	5	binding	binding	NN
cord-333636-h2sg6shp	270	6	was	be	VBD
cord-333636-h2sg6shp	270	7	severely	severely	RB
cord-333636-h2sg6shp	270	8	impaired	impair	VBN
cord-333636-h2sg6shp	270	9	by	by	IN
cord-333636-h2sg6shp	270	10	the	the	DT
cord-333636-h2sg6shp	270	11	deletion	deletion	NN
cord-333636-h2sg6shp	270	12	of	of	IN
cord-333636-h2sg6shp	270	13	a	a	DT
cord-333636-h2sg6shp	270	14	short	short	JJ
cord-333636-h2sg6shp	270	15	,	,	,
cord-333636-h2sg6shp	270	16	highly	highly	RB
cord-333636-h2sg6shp	270	17	conserved	conserve	VBN
cord-333636-h2sg6shp	270	18	sequence	sequence	NN
cord-333636-h2sg6shp	270	19	between	between	IN
cord-333636-h2sg6shp	270	20	residues	residue	NNS
cord-333636-h2sg6shp	270	21	856	856	CD
cord-333636-h2sg6shp	270	22	-	-	HYPH
cord-333636-h2sg6shp	270	23	879	879	CD
cord-333636-h2sg6shp	270	24	.	.	.
cord-333636-h2sg6shp	271	1	Whether	whether	IN
cord-333636-h2sg6shp	271	2	this	this	DT
cord-333636-h2sg6shp	271	3	region	region	NN
cord-333636-h2sg6shp	271	4	is	be	VBZ
cord-333636-h2sg6shp	271	5	directly	directly	RB
cord-333636-h2sg6shp	271	6	involved	involve	VBN
cord-333636-h2sg6shp	271	7	in	in	IN
cord-333636-h2sg6shp	271	8	binding	bind	VBG
cord-333636-h2sg6shp	271	9	to	to	IN
cord-333636-h2sg6shp	271	10	ssRNA	ssrna	ADD
cord-333636-h2sg6shp	272	1	and	and	CC
cord-333636-h2sg6shp	272	2	dsRNA	dsrna	NN
cord-333636-h2sg6shp	272	3	,	,	,
cord-333636-h2sg6shp	272	4	is	be	VBZ
cord-333636-h2sg6shp	272	5	a	a	DT
cord-333636-h2sg6shp	272	6	subject	subject	NN
cord-333636-h2sg6shp	272	7	of	of	IN
cord-333636-h2sg6shp	272	8	investigation	investigation	NN
cord-333636-h2sg6shp	272	9	.	.	.
cord-333636-h2sg6shp	273	1	To	to	IN
cord-333636-h2sg6shp	273	2	our	-PRON-	PRP$
cord-333636-h2sg6shp	273	3	knowledge	knowledge	NN
cord-333636-h2sg6shp	273	4	,	,	,
cord-333636-h2sg6shp	273	5	this	this	DT
cord-333636-h2sg6shp	273	6	is	be	VBZ
cord-333636-h2sg6shp	273	7	the	the	DT
cord-333636-h2sg6shp	273	8	first	first	JJ
cord-333636-h2sg6shp	273	9	time	time	NN
cord-333636-h2sg6shp	273	10	that	that	WDT
cord-333636-h2sg6shp	273	11	an	an	DT
cord-333636-h2sg6shp	273	12	RNA	RNA	NNP
cord-333636-h2sg6shp	273	13	-	-	HYPH
cord-333636-h2sg6shp	273	14	binding	bind	VBG
cord-333636-h2sg6shp	273	15	activity	activity	NN
cord-333636-h2sg6shp	273	16	has	have	VBZ
cord-333636-h2sg6shp	273	17	been	be	VBN
cord-333636-h2sg6shp	273	18	demonstrated	demonstrate	VBN
cord-333636-h2sg6shp	273	19	for	for	IN
cord-333636-h2sg6shp	273	20	a	a	DT
cord-333636-h2sg6shp	273	21	birnavirus	birnavirus	JJ
cord-333636-h2sg6shp	273	22	VP3	VP3	NNP
cord-333636-h2sg6shp	273	23	protein	protein	NN
cord-333636-h2sg6shp	273	24	.	.	.
cord-333636-h2sg6shp	274	1	VP3	VP3	NNP
cord-333636-h2sg6shp	274	2	protein	protein	NN
cord-333636-h2sg6shp	274	3	bound	bind	VBN
cord-333636-h2sg6shp	274	4	ssRNA	ssrna	NN
cord-333636-h2sg6shp	274	5	and	and	CC
cord-333636-h2sg6shp	274	6	dsRNA	dsRNA	NNS
cord-333636-h2sg6shp	274	7	,	,	,
cord-333636-h2sg6shp	274	8	exhibiting	exhibit	VBG
cord-333636-h2sg6shp	274	9	no	no	DT
cord-333636-h2sg6shp	274	10	specificity	specificity	NN
cord-333636-h2sg6shp	274	11	for	for	IN
cord-333636-h2sg6shp	274	12	virus	virus	NN
cord-333636-h2sg6shp	274	13	-	-	HYPH
cord-333636-h2sg6shp	274	14	derived	derive	VBN
cord-333636-h2sg6shp	274	15	ssRNA	ssrna	NN
cord-333636-h2sg6shp	274	16	probes	probe	NNS
cord-333636-h2sg6shp	274	17	.	.	.
cord-333636-h2sg6shp	275	1	The	the	DT
cord-333636-h2sg6shp	275	2	identification	identification	NN
cord-333636-h2sg6shp	275	3	of	of	IN
cord-333636-h2sg6shp	275	4	critical	critical	JJ
cord-333636-h2sg6shp	275	5	residues	residue	NNS
cord-333636-h2sg6shp	275	6	for	for	IN
cord-333636-h2sg6shp	275	7	RNA	rna	NN
cord-333636-h2sg6shp	275	8	-	-	HYPH
cord-333636-h2sg6shp	275	9	binding	bind	VBG
cord-333636-h2sg6shp	275	10	activity	activity	NN
cord-333636-h2sg6shp	275	11	within	within	IN
cord-333636-h2sg6shp	275	12	VP3	VP3	NNP
cord-333636-h2sg6shp	275	13	RNA	RNA	NNP
cord-333636-h2sg6shp	275	14	-	-	HYPH
cord-333636-h2sg6shp	275	15	binding	bind	VBG
cord-333636-h2sg6shp	275	16	domain	domain	NN
cord-333636-h2sg6shp	275	17	is	be	VBZ
cord-333636-h2sg6shp	275	18	currently	currently	RB
cord-333636-h2sg6shp	275	19	under	under	IN
cord-333636-h2sg6shp	275	20	investigation	investigation	NN
cord-333636-h2sg6shp	275	21	.	.	.
cord-333636-h2sg6shp	276	1	The	the	DT
cord-333636-h2sg6shp	276	2	implications	implication	NNS
cord-333636-h2sg6shp	276	3	of	of	IN
cord-333636-h2sg6shp	276	4	VP3	VP3	NNP
cord-333636-h2sg6shp	276	5	RNA	RNA	NNP
cord-333636-h2sg6shp	276	6	-	-	HYPH
cord-333636-h2sg6shp	276	7	binding	bind	VBG
cord-333636-h2sg6shp	276	8	activity	activity	NN
cord-333636-h2sg6shp	276	9	are	be	VBP
cord-333636-h2sg6shp	276	10	also	also	RB
cord-333636-h2sg6shp	276	11	being	be	VBG
cord-333636-h2sg6shp	276	12	investigated	investigate	VBN
cord-333636-h2sg6shp	276	13	in	in	IN
cord-333636-h2sg6shp	276	14	the	the	DT
cord-333636-h2sg6shp	276	15	context	context	NN
cord-333636-h2sg6shp	276	16	of	of	IN
cord-333636-h2sg6shp	276	17	the	the	DT
cord-333636-h2sg6shp	276	18	virus	virus	NN
cord-333636-h2sg6shp	276	19	replication	replication	NN
cord-333636-h2sg6shp	276	20	and	and	CC
cord-333636-h2sg6shp	276	21	transcription	transcription	NN
cord-333636-h2sg6shp	276	22	.	.	.
cord-333636-h2sg6shp	277	1	Nuclear	nuclear	JJ
cord-333636-h2sg6shp	277	2	factor	factor	NN
cord-333636-h2sg6shp	278	1	I	-PRON-	PRP
cord-333636-h2sg6shp	278	2	family	family	NN
cord-333636-h2sg6shp	278	3	members	member	NNS
cord-333636-h2sg6shp	278	4	regulate	regulate	VBP
cord-333636-h2sg6shp	278	5	the	the	DT
cord-333636-h2sg6shp	278	6	transcription	transcription	NN
cord-333636-h2sg6shp	278	7	of	of	IN
cord-333636-h2sg6shp	278	8	surfactant	surfactant	NN
cord-333636-h2sg6shp	278	9	protein	protein	NN
cord-333636-h2sg6shp	278	10	-	-	HYPH
cord-333636-h2sg6shp	278	11	C	C	NNP
cord-333636-h2sg6shp	278	12	Adding	add	VBG
cord-333636-h2sg6shp	278	13	the	the	DT
cord-333636-h2sg6shp	278	14	third	third	JJ
cord-333636-h2sg6shp	278	15	dimension	dimension	NN
cord-333636-h2sg6shp	278	16	to	to	IN
cord-333636-h2sg6shp	278	17	virus	virus	NN
cord-333636-h2sg6shp	278	18	life	life	NN
cord-333636-h2sg6shp	278	19	cycles	cycle	NNS
cord-333636-h2sg6shp	278	20	:	:	:
cord-333636-h2sg6shp	278	21	three	three	CD
cord-333636-h2sg6shp	278	22	-	-	HYPH
cord-333636-h2sg6shp	278	23	dimensional	dimensional	JJ
cord-333636-h2sg6shp	278	24	reconstructions	reconstruction	NNS
cord-333636-h2sg6shp	278	25	of	of	IN
cord-333636-h2sg6shp	278	26	icosaedral	icosaedral	JJ
cord-333636-h2sg6shp	278	27	viruses	virus	NNS
cord-333636-h2sg6shp	278	28	from	from	IN
cord-333636-h2sg6shp	278	29	cryoelectron	cryoelectron	NNP
cord-333636-h2sg6shp	278	30	micrographs	micrograph	NNS
cord-333636-h2sg6shp	278	31	Relationships	relationship	NNS
cord-333636-h2sg6shp	278	32	between	between	IN
cord-333636-h2sg6shp	278	33	the	the	DT
cord-333636-h2sg6shp	278	34	magnitude	magnitude	NN
cord-333636-h2sg6shp	278	35	of	of	IN
cord-333636-h2sg6shp	278	36	Hill	Hill	NNP
cord-333636-h2sg6shp	278	37	plot	plot	NN
cord-333636-h2sg6shp	278	38	slopes	slope	NNS
cord-333636-h2sg6shp	278	39	,	,	,
cord-333636-h2sg6shp	278	40	apparent	apparent	JJ
cord-333636-h2sg6shp	278	41	binding	bind	VBG
cord-333636-h2sg6shp	278	42	constants	constant	NNS
cord-333636-h2sg6shp	278	43	and	and	CC
cord-333636-h2sg6shp	278	44	factorability	factorability	NN
cord-333636-h2sg6shp	278	45	of	of	IN
cord-333636-h2sg6shp	278	46	bindings	binding	NNS
cord-333636-h2sg6shp	278	47	polynomials	polynomial	NNS
cord-333636-h2sg6shp	278	48	and	and	CC
cord-333636-h2sg6shp	278	49	their	-PRON-	PRP$
cord-333636-h2sg6shp	278	50	Hessians	Hessians	NNPS
cord-333636-h2sg6shp	278	51	A	a	DT
cord-333636-h2sg6shp	278	52	non	non	JJ
cord-333636-h2sg6shp	278	53	-	-	JJ
cord-333636-h2sg6shp	278	54	canonical	canonical	JJ
cord-333636-h2sg6shp	278	55	lon	lon	NN
cord-333636-h2sg6shp	278	56	proteinase	proteinase	NN
cord-333636-h2sg6shp	278	57	lacking	lack	VBG
cord-333636-h2sg6shp	278	58	the	the	DT
cord-333636-h2sg6shp	278	59	ATPase	ATPase	NNP
cord-333636-h2sg6shp	278	60	domain	domain	NN
cord-333636-h2sg6shp	278	61	employs	employ	VBZ
cord-333636-h2sg6shp	278	62	the	the	DT
cord-333636-h2sg6shp	278	63	ser	ser	NNP
cord-333636-h2sg6shp	278	64	-	-	HYPH
cord-333636-h2sg6shp	278	65	Lys	Lys	NNP
cord-333636-h2sg6shp	278	66	catalytic	catalytic	JJ
cord-333636-h2sg6shp	278	67	dyad	dyad	NN
cord-333636-h2sg6shp	278	68	to	to	TO
cord-333636-h2sg6shp	278	69	exercise	exercise	VB
cord-333636-h2sg6shp	278	70	broad	broad	JJ
cord-333636-h2sg6shp	278	71	control	control	NN
cord-333636-h2sg6shp	278	72	over	over	IN
cord-333636-h2sg6shp	278	73	the	the	DT
cord-333636-h2sg6shp	278	74	life	life	NN
cord-333636-h2sg6shp	278	75	cycle	cycle	NN
cord-333636-h2sg6shp	278	76	of	of	IN
cord-333636-h2sg6shp	278	77	a	a	DT
cord-333636-h2sg6shp	278	78	double	double	RB
cord-333636-h2sg6shp	278	79	-	-	HYPH
cord-333636-h2sg6shp	278	80	stranded	strand	VBN
cord-333636-h2sg6shp	278	81	RNA	RNA	NNP
cord-333636-h2sg6shp	278	82	virus	virus	NN
cord-333636-h2sg6shp	279	1	The	the	DT
cord-333636-h2sg6shp	279	2	electrophoretic	electrophoretic	JJ
cord-333636-h2sg6shp	279	3	mobility	mobility	NN
cord-333636-h2sg6shp	279	4	shift	shift	NN
cord-333636-h2sg6shp	279	5	assay	assay	NN
cord-333636-h2sg6shp	279	6	for	for	IN
cord-333636-h2sg6shp	279	7	RNA	rna	NN
cord-333636-h2sg6shp	279	8	-	-	HYPH
cord-333636-h2sg6shp	279	9	binding	bind	VBG
cord-333636-h2sg6shp	279	10	proteins	protein	NNS
cord-333636-h2sg6shp	279	11	Three	three	CD
cord-333636-h2sg6shp	279	12	-	-	HYPH
cord-333636-h2sg6shp	279	13	dimensional	dimensional	JJ
cord-333636-h2sg6shp	279	14	structure	structure	NN
cord-333636-h2sg6shp	279	15	of	of	IN
cord-333636-h2sg6shp	279	16	infectious	infectious	JJ
cord-333636-h2sg6shp	279	17	bursal	bursal	NN
cord-333636-h2sg6shp	279	18	disease	disease	NN
cord-333636-h2sg6shp	279	19	virus	virus	NN
cord-333636-h2sg6shp	279	20	determined	determine	VBN
cord-333636-h2sg6shp	279	21	by	by	IN
cord-333636-h2sg6shp	279	22	electron	electron	NN
cord-333636-h2sg6shp	279	23	cryomicroscopy	cryomicroscopy	NNP
cord-333636-h2sg6shp	279	24	Relationships	relationship	NNS
cord-333636-h2sg6shp	279	25	among	among	IN
cord-333636-h2sg6shp	279	26	the	the	DT
cord-333636-h2sg6shp	279	27	positive	positive	JJ
cord-333636-h2sg6shp	279	28	strand	strand	NN
cord-333636-h2sg6shp	279	29	and	and	CC
cord-333636-h2sg6shp	279	30	double	double	JJ
cord-333636-h2sg6shp	279	31	-	-	HYPH
cord-333636-h2sg6shp	279	32	strand	strand	NN
cord-333636-h2sg6shp	279	33	RNA	RNA	NNP
cord-333636-h2sg6shp	279	34	viruses	virus	NNS
cord-333636-h2sg6shp	279	35	as	as	IN
cord-333636-h2sg6shp	279	36	viewed	view	VBN
cord-333636-h2sg6shp	279	37	through	through	IN
cord-333636-h2sg6shp	279	38	their	-PRON-	PRP$
cord-333636-h2sg6shp	279	39	RNA	RNA	NNP
cord-333636-h2sg6shp	279	40	-	-	HYPH
cord-333636-h2sg6shp	279	41	dependent	dependent	JJ
cord-333636-h2sg6shp	279	42	RNA	RNA	NNP
cord-333636-h2sg6shp	279	43	polymerases	polymerase	NNS
cord-333636-h2sg6shp	279	44	Conserved	conserve	VBN
cord-333636-h2sg6shp	279	45	structures	structure	NNS
cord-333636-h2sg6shp	279	46	and	and	CC
cord-333636-h2sg6shp	279	47	diversity	diversity	NN
cord-333636-h2sg6shp	279	48	of	of	IN
cord-333636-h2sg6shp	279	49	functions	function	NNS
cord-333636-h2sg6shp	279	50	of	of	IN
cord-333636-h2sg6shp	279	51	RNAbinding	rnabinding	NN
cord-333636-h2sg6shp	279	52	proteins	protein	NNS
cord-333636-h2sg6shp	279	53	C	C	NNP
cord-333636-h2sg6shp	279	54	terminus	terminus	NN
cord-333636-h2sg6shp	279	55	of	of	IN
cord-333636-h2sg6shp	279	56	infectious	infectious	JJ
cord-333636-h2sg6shp	279	57	bursal	bursal	NNP
cord-333636-h2sg6shp	279	58	disease	disease	NNP
cord-333636-h2sg6shp	279	59	virus	virus	NN
cord-333636-h2sg6shp	279	60	major	major	JJ
cord-333636-h2sg6shp	279	61	capsid	capsid	NN
cord-333636-h2sg6shp	279	62	protein	protein	NN
cord-333636-h2sg6shp	279	63	VP2	vp2	NN
cord-333636-h2sg6shp	279	64	is	be	VBZ
cord-333636-h2sg6shp	279	65	involved	involve	VBN
cord-333636-h2sg6shp	279	66	in	in	IN
cord-333636-h2sg6shp	279	67	definition	definition	NN
cord-333636-h2sg6shp	279	68	of	of	IN
cord-333636-h2sg6shp	279	69	the	the	DT
cord-333636-h2sg6shp	279	70	T	T	NNP
cord-333636-h2sg6shp	279	71	number	number	NN
cord-333636-h2sg6shp	279	72	for	for	IN
cord-333636-h2sg6shp	279	73	capsid	capsid	NNP
cord-333636-h2sg6shp	279	74	assembly	assembly	NN
cord-333636-h2sg6shp	280	1	The	the	DT
cord-333636-h2sg6shp	280	2	maturation	maturation	NN
cord-333636-h2sg6shp	280	3	process	process	NN
cord-333636-h2sg6shp	280	4	of	of	IN
cord-333636-h2sg6shp	280	5	pVP2	pVP2	NNP
cord-333636-h2sg6shp	280	6	requires	require	VBZ
cord-333636-h2sg6shp	280	7	assembly	assembly	NN
cord-333636-h2sg6shp	280	8	of	of	IN
cord-333636-h2sg6shp	280	9	infectious	infectious	JJ
cord-333636-h2sg6shp	280	10	bursal	bursal	NNP
cord-333636-h2sg6shp	280	11	disease	disease	NNP
cord-333636-h2sg6shp	280	12	virus	virus	NNP
cord-333636-h2sg6shp	280	13	capsids	capsids	NNP
cord-333636-h2sg6shp	281	1	The	the	DT
cord-333636-h2sg6shp	281	2	capsid	capsid	NN
cord-333636-h2sg6shp	281	3	of	of	IN
cord-333636-h2sg6shp	281	4	infectious	infectious	JJ
cord-333636-h2sg6shp	281	5	bursal	bursal	NN
cord-333636-h2sg6shp	281	6	disease	disease	NN
cord-333636-h2sg6shp	281	7	virus	virus	NN
cord-333636-h2sg6shp	281	8	contains	contain	VBZ
cord-333636-h2sg6shp	281	9	several	several	JJ
cord-333636-h2sg6shp	281	10	small	small	JJ
cord-333636-h2sg6shp	281	11	peptides	peptide	NNS
cord-333636-h2sg6shp	281	12	arising	arise	VBG
cord-333636-h2sg6shp	281	13	from	from	IN
cord-333636-h2sg6shp	281	14	the	the	DT
cord-333636-h2sg6shp	281	15	maturation	maturation	NN
cord-333636-h2sg6shp	281	16	process	process	NN
cord-333636-h2sg6shp	281	17	of	of	IN
cord-333636-h2sg6shp	281	18	pVP2	pVP2	NNP
cord-333636-h2sg6shp	281	19	A	a	DT
cord-333636-h2sg6shp	281	20	model	model	NN
cord-333636-h2sg6shp	281	21	of	of	IN
cord-333636-h2sg6shp	281	22	evolutionary	evolutionary	JJ
cord-333636-h2sg6shp	281	23	change	change	NN
cord-333636-h2sg6shp	281	24	in	in	IN
cord-333636-h2sg6shp	281	25	proteins	protein	NNS
cord-333636-h2sg6shp	281	26	,	,	,
cord-333636-h2sg6shp	281	27	matrixes	matrix	NNS
cord-333636-h2sg6shp	281	28	for	for	IN
cord-333636-h2sg6shp	281	29	detecting	detect	VBG
cord-333636-h2sg6shp	281	30	distant	distant	JJ
cord-333636-h2sg6shp	281	31	relationships	relationship	NNS
cord-333636-h2sg6shp	281	32	Biophysical	biophysical	JJ
cord-333636-h2sg6shp	281	33	and	and	CC
cord-333636-h2sg6shp	281	34	biochemical	biochemical	JJ
cord-333636-h2sg6shp	281	35	characterization	characterization	NN
cord-333636-h2sg6shp	281	36	of	of	IN
cord-333636-h2sg6shp	281	37	five	five	CD
cord-333636-h2sg6shp	281	38	animal	animal	NN
cord-333636-h2sg6shp	281	39	viruses	virus	NNS
cord-333636-h2sg6shp	281	40	with	with	IN
cord-333636-h2sg6shp	281	41	bisegmented	bisegmented	JJ
cord-333636-h2sg6shp	281	42	double	double	RB
cord-333636-h2sg6shp	281	43	-	-	HYPH
cord-333636-h2sg6shp	281	44	stranded	strand	VBN
cord-333636-h2sg6shp	281	45	RNA	RNA	NNP
cord-333636-h2sg6shp	281	46	genomes	genome	NNS
cord-333636-h2sg6shp	281	47	Themes	Themes	NNP
cord-333636-h2sg6shp	281	48	in	in	IN
cord-333636-h2sg6shp	281	49	RNA	rna	NN
cord-333636-h2sg6shp	281	50	-	-	HYPH
cord-333636-h2sg6shp	281	51	protein	protein	NN
cord-333636-h2sg6shp	281	52	recognition	recognition	NN
cord-333636-h2sg6shp	282	1	The	the	DT
cord-333636-h2sg6shp	282	2	viral	viral	JJ
cord-333636-h2sg6shp	282	3	nucleocapsid	nucleocapsid	NN
cord-333636-h2sg6shp	282	4	protein	protein	NN
cord-333636-h2sg6shp	282	5	of	of	IN
cord-333636-h2sg6shp	282	6	transmissible	transmissible	JJ
cord-333636-h2sg6shp	282	7	gastroenteritis	gastroenteritis	NN
cord-333636-h2sg6shp	282	8	coronavirus	coronavirus	NN
cord-333636-h2sg6shp	282	9	(	(	-LRB-
cord-333636-h2sg6shp	282	10	TGEV	TGEV	NNP
cord-333636-h2sg6shp	282	11	)	)	-RRB-
cord-333636-h2sg6shp	282	12	is	be	VBZ
cord-333636-h2sg6shp	282	13	cleaved	cleave	VBN
cord-333636-h2sg6shp	282	14	by	by	IN
cord-333636-h2sg6shp	282	15	caspase-6	caspase-6	NNP
cord-333636-h2sg6shp	283	1	and	and	CC
cord-333636-h2sg6shp	283	2	-7	-7	NNP
cord-333636-h2sg6shp	283	3	during	during	IN
cord-333636-h2sg6shp	283	4	TGEV	TGEV	NNP
cord-333636-h2sg6shp	283	5	-	-	HYPH
cord-333636-h2sg6shp	283	6	induced	induce	VBN
cord-333636-h2sg6shp	283	7	apoptosis	apoptosis	NN
cord-333636-h2sg6shp	284	1	The	the	DT
cord-333636-h2sg6shp	284	2	major	major	JJ
cord-333636-h2sg6shp	284	3	antigenic	antigenic	JJ
cord-333636-h2sg6shp	284	4	protein	protein	NN
cord-333636-h2sg6shp	284	5	of	of	IN
cord-333636-h2sg6shp	284	6	infectious	infectious	JJ
cord-333636-h2sg6shp	284	7	bursal	bursal	NN
cord-333636-h2sg6shp	284	8	disease	disease	NN
cord-333636-h2sg6shp	284	9	virus	virus	NN
cord-333636-h2sg6shp	284	10	,	,	,
cord-333636-h2sg6shp	284	11	VP2	VP2	NNP
cord-333636-h2sg6shp	284	12	,	,	,
cord-333636-h2sg6shp	284	13	is	be	VBZ
cord-333636-h2sg6shp	284	14	an	an	DT
cord-333636-h2sg6shp	284	15	apoptotic	apoptotic	JJ
cord-333636-h2sg6shp	284	16	inducer	inducer	NN
cord-333636-h2sg6shp	284	17	Expression	expression	NN
cord-333636-h2sg6shp	284	18	of	of	IN
cord-333636-h2sg6shp	284	19	ORF	ORF	NNP
cord-333636-h2sg6shp	284	20	A1	A1	NNP
cord-333636-h2sg6shp	284	21	of	of	IN
cord-333636-h2sg6shp	284	22	infectious	infectious	JJ
cord-333636-h2sg6shp	284	23	bursal	bursal	NN
cord-333636-h2sg6shp	284	24	disease	disease	NN
cord-333636-h2sg6shp	284	25	virus	virus	NN
cord-333636-h2sg6shp	284	26	results	result	VBZ
cord-333636-h2sg6shp	284	27	in	in	IN
cord-333636-h2sg6shp	284	28	the	the	DT
cord-333636-h2sg6shp	284	29	formation	formation	NN
cord-333636-h2sg6shp	284	30	of	of	IN
cord-333636-h2sg6shp	284	31	virus	virus	NN
cord-333636-h2sg6shp	284	32	-	-	HYPH
cord-333636-h2sg6shp	284	33	like	like	JJ
cord-333636-h2sg6shp	284	34	particles	particle	NNS
cord-333636-h2sg6shp	284	35	Poultry	poultry	NN
cord-333636-h2sg6shp	284	36	virus	virus	NN
cord-333636-h2sg6shp	284	37	infection	infection	NN
cord-333636-h2sg6shp	284	38	in	in	IN
cord-333636-h2sg6shp	284	39	Antarctic	antarctic	JJ
cord-333636-h2sg6shp	284	40	penguins	penguin	NNS
cord-333636-h2sg6shp	284	41	Characterization	characterization	NN
cord-333636-h2sg6shp	284	42	of	of	IN
cord-333636-h2sg6shp	284	43	influenza	influenza	NN
cord-333636-h2sg6shp	284	44	virus	virus	NN
cord-333636-h2sg6shp	284	45	PB1	PB1	NNP
cord-333636-h2sg6shp	284	46	protein	protein	NN
cord-333636-h2sg6shp	284	47	binding	bind	VBG
cord-333636-h2sg6shp	284	48	to	to	IN
cord-333636-h2sg6shp	284	49	viral	viral	JJ
cord-333636-h2sg6shp	284	50	RNA	RNA	NNP
cord-333636-h2sg6shp	284	51	:	:	:
cord-333636-h2sg6shp	284	52	two	two	CD
cord-333636-h2sg6shp	284	53	separate	separate	JJ
cord-333636-h2sg6shp	284	54	regions	region	NNS
cord-333636-h2sg6shp	284	55	of	of	IN
cord-333636-h2sg6shp	284	56	the	the	DT
cord-333636-h2sg6shp	284	57	protein	protein	NN
cord-333636-h2sg6shp	284	58	contribute	contribute	VBP
cord-333636-h2sg6shp	284	59	to	to	IN
cord-333636-h2sg6shp	284	60	the	the	DT
cord-333636-h2sg6shp	284	61	interaction	interaction	NN
cord-333636-h2sg6shp	284	62	domain	domain	NN
cord-333636-h2sg6shp	284	63	RNA	RNA	NNP
cord-333636-h2sg6shp	284	64	binding	binding	NN
cord-333636-h2sg6shp	284	65	of	of	IN
cord-333636-h2sg6shp	284	66	recombinant	recombinant	JJ
cord-333636-h2sg6shp	284	67	nucleocapsid	nucleocapsid	NN
cord-333636-h2sg6shp	284	68	proteins	protein	NNS
cord-333636-h2sg6shp	284	69	of	of	IN
cord-333636-h2sg6shp	284	70	hantaviruses	hantaviruse	NNS
cord-333636-h2sg6shp	284	71	Nitrocellulose	Nitrocellulose	NNP
cord-333636-h2sg6shp	284	72	filter	filter	NN
cord-333636-h2sg6shp	284	73	binding	bind	VBG
cord-333636-h2sg6shp	284	74	for	for	IN
cord-333636-h2sg6shp	284	75	determination	determination	NN
cord-333636-h2sg6shp	284	76	of	of	IN
cord-333636-h2sg6shp	284	77	dissociation	dissociation	NN
cord-333636-h2sg6shp	284	78	constants	constant	NNS
cord-333636-h2sg6shp	284	79	Infectious	infectious	JJ
cord-333636-h2sg6shp	284	80	pancreatic	pancreatic	JJ
cord-333636-h2sg6shp	284	81	necrosis	necrosis	NN
cord-333636-h2sg6shp	284	82	virus	virus	NN
cord-333636-h2sg6shp	284	83	:	:	:
cord-333636-h2sg6shp	284	84	identification	identification	NN
cord-333636-h2sg6shp	284	85	of	of	IN
cord-333636-h2sg6shp	284	86	a	a	DT
cord-333636-h2sg6shp	284	87	VP3-containing	vp3-containe	VBG
cord-333636-h2sg6shp	284	88	ribonucleoprotein	ribonucleoprotein	NN
cord-333636-h2sg6shp	284	89	core	core	NN
cord-333636-h2sg6shp	284	90	structure	structure	NN
cord-333636-h2sg6shp	284	91	and	and	CC
cord-333636-h2sg6shp	284	92	evidence	evidence	NN
cord-333636-h2sg6shp	284	93	for	for	IN
cord-333636-h2sg6shp	284	94	O	o	NN
cord-333636-h2sg6shp	284	95	-	-	HYPH
cord-333636-h2sg6shp	284	96	linked	link	VBN
cord-333636-h2sg6shp	284	97	glycosylation	glycosylation	NN
cord-333636-h2sg6shp	284	98	of	of	IN
cord-333636-h2sg6shp	284	99	the	the	DT
cord-333636-h2sg6shp	284	100	capsid	capsid	NN
cord-333636-h2sg6shp	284	101	protein	protein	NN
cord-333636-h2sg6shp	284	102	VP2	VP2	NNP
cord-333636-h2sg6shp	284	103	Identification	Identification	NNP
cord-333636-h2sg6shp	284	104	of	of	IN
cord-333636-h2sg6shp	284	105	IPNV	IPNV	NNP
cord-333636-h2sg6shp	284	106	-	-	HYPH
cord-333636-h2sg6shp	284	107	specified	specify	VBN
cord-333636-h2sg6shp	284	108	components	component	NNS
cord-333636-h2sg6shp	284	109	released	release	VBN
cord-333636-h2sg6shp	284	110	from	from	IN
cord-333636-h2sg6shp	284	111	productively	productively	RB
cord-333636-h2sg6shp	284	112	infected	infect	VBN
cord-333636-h2sg6shp	284	113	RTG-2	RTG-2	NNP
cord-333636-h2sg6shp	284	114	cells	cell	NNS
cord-333636-h2sg6shp	284	115	following	follow	VBG
cord-333636-h2sg6shp	284	116	massive	massive	JJ
cord-333636-h2sg6shp	284	117	cytopathic	cytopathic	JJ
cord-333636-h2sg6shp	284	118	effect	effect	NN
cord-333636-h2sg6shp	284	119	Genomic	genomic	JJ
cord-333636-h2sg6shp	284	120	structure	structure	NN
cord-333636-h2sg6shp	284	121	of	of	IN
cord-333636-h2sg6shp	284	122	the	the	DT
cord-333636-h2sg6shp	284	123	large	large	JJ
cord-333636-h2sg6shp	284	124	RNA	RNA	NNP
cord-333636-h2sg6shp	284	125	segment	segment	NN
cord-333636-h2sg6shp	284	126	of	of	IN
cord-333636-h2sg6shp	284	127	infectious	infectious	JJ
cord-333636-h2sg6shp	284	128	bursal	bursal	NN
cord-333636-h2sg6shp	284	129	disease	disease	NN
cord-333636-h2sg6shp	284	130	virus	virus	NN
cord-333636-h2sg6shp	284	131	Evidence	evidence	NN
cord-333636-h2sg6shp	284	132	that	that	IN
cord-333636-h2sg6shp	284	133	virion	virion	NN
cord-333636-h2sg6shp	284	134	-	-	HYPH
cord-333636-h2sg6shp	284	135	associated	associate	VBN
cord-333636-h2sg6shp	284	136	VP1	vp1	NN
cord-333636-h2sg6shp	284	137	of	of	IN
cord-333636-h2sg6shp	284	138	Avibirnaviruses	Avibirnaviruses	NNP
cord-333636-h2sg6shp	284	139	contains	contain	VBZ
cord-333636-h2sg6shp	284	140	viral	viral	JJ
cord-333636-h2sg6shp	284	141	RNA	RNA	NNP
cord-333636-h2sg6shp	284	142	sequences	sequence	NNS
cord-333636-h2sg6shp	284	143	Echovirus-9	Echovirus-9	NNP
cord-333636-h2sg6shp	284	144	protein	protein	NN
cord-333636-h2sg6shp	284	145	2C	2C	NNP
cord-333636-h2sg6shp	284	146	binds	bind	VBZ
cord-333636-h2sg6shp	284	147	single	single	RB
cord-333636-h2sg6shp	284	148	-	-	HYPH
cord-333636-h2sg6shp	284	149	stranded	strand	VBN
cord-333636-h2sg6shp	284	150	RNA	RNA	NNP
cord-333636-h2sg6shp	284	151	unspecifically	unspecifically	NN
cord-333636-h2sg6shp	284	152	Family	Family	NNP
cord-333636-h2sg6shp	284	153	Birnaviridae	Birnaviridae	NNP
cord-333636-h2sg6shp	284	154	VP1	VP1	NNP
cord-333636-h2sg6shp	284	155	,	,	,
cord-333636-h2sg6shp	284	156	the	the	DT
cord-333636-h2sg6shp	284	157	putative	putative	JJ
cord-333636-h2sg6shp	284	158	RNA	RNA	NNP
cord-333636-h2sg6shp	284	159	-	-	HYPH
cord-333636-h2sg6shp	284	160	dependent	dependent	JJ
cord-333636-h2sg6shp	284	161	RNA	RNA	NNP
cord-333636-h2sg6shp	284	162	polymerase	polymerase	NN
cord-333636-h2sg6shp	284	163	of	of	IN
cord-333636-h2sg6shp	284	164	infectious	infectious	JJ
cord-333636-h2sg6shp	284	165	bursal	bursal	NN
cord-333636-h2sg6shp	284	166	disease	disease	NN
cord-333636-h2sg6shp	284	167	virus	virus	NN
cord-333636-h2sg6shp	284	168	,	,	,
cord-333636-h2sg6shp	284	169	forms	form	VBZ
cord-333636-h2sg6shp	284	170	complexes	complex	NNS
cord-333636-h2sg6shp	284	171	with	with	IN
cord-333636-h2sg6shp	284	172	the	the	DT
cord-333636-h2sg6shp	284	173	capsid	capsid	NN
cord-333636-h2sg6shp	284	174	protein	protein	NN
cord-333636-h2sg6shp	284	175	VP3	VP3	NNP
cord-333636-h2sg6shp	284	176	,	,	,
cord-333636-h2sg6shp	284	177	leading	lead	VBG
cord-333636-h2sg6shp	284	178	to	to	IN
cord-333636-h2sg6shp	284	179	efficient	efficient	JJ
cord-333636-h2sg6shp	284	180	encapsidation	encapsidation	NN
cord-333636-h2sg6shp	284	181	into	into	IN
cord-333636-h2sg6shp	284	182	virus	virus	NN
cord-333636-h2sg6shp	284	183	-	-	HYPH
cord-333636-h2sg6shp	284	184	like	like	JJ
cord-333636-h2sg6shp	284	185	particles	particle	NNS
cord-333636-h2sg6shp	284	186	VP5	VP5	NNS
cord-333636-h2sg6shp	284	187	,	,	,
cord-333636-h2sg6shp	284	188	the	the	DT
cord-333636-h2sg6shp	284	189	nonstructural	nonstructural	JJ
cord-333636-h2sg6shp	284	190	polypeptide	polypeptide	NN
cord-333636-h2sg6shp	284	191	of	of	IN
cord-333636-h2sg6shp	284	192	infectious	infectious	JJ
cord-333636-h2sg6shp	284	193	bursal	bursal	NN
cord-333636-h2sg6shp	284	194	disease	disease	NN
cord-333636-h2sg6shp	284	195	virus	virus	NN
cord-333636-h2sg6shp	284	196	,	,	,
cord-333636-h2sg6shp	284	197	accumulates	accumulate	VBZ
cord-333636-h2sg6shp	284	198	within	within	IN
cord-333636-h2sg6shp	284	199	the	the	DT
cord-333636-h2sg6shp	284	200	host	host	NN
cord-333636-h2sg6shp	284	201	plasma	plasma	NN
cord-333636-h2sg6shp	284	202	membrane	membrane	NN
cord-333636-h2sg6shp	284	203	and	and	CC
cord-333636-h2sg6shp	284	204	induces	induce	VBZ
cord-333636-h2sg6shp	284	205	cell	cell	NN
cord-333636-h2sg6shp	284	206	lysis	lysis	NN
cord-333636-h2sg6shp	284	207	Expression	Expression	NNP
cord-333636-h2sg6shp	284	208	and	and	CC
cord-333636-h2sg6shp	284	209	RNA	RNA	NNP
cord-333636-h2sg6shp	284	210	dependent	dependent	JJ
cord-333636-h2sg6shp	284	211	RNA	RNA	NNP
cord-333636-h2sg6shp	284	212	polymerase	polymerase	NN
cord-333636-h2sg6shp	284	213	activity	activity	NN
cord-333636-h2sg6shp	284	214	of	of	IN
cord-333636-h2sg6shp	284	215	birnavirus	birnavirus	NNP
cord-333636-h2sg6shp	284	216	VP1	VP1	NNP
cord-333636-h2sg6shp	284	217	protein	protein	NN
cord-333636-h2sg6shp	284	218	in	in	IN
cord-333636-h2sg6shp	284	219	bacteria	bacteria	NNS
cord-333636-h2sg6shp	284	220	and	and	CC
cord-333636-h2sg6shp	284	221	yeast	yeast	NN
cord-333636-h2sg6shp	285	1	In	in	IN
cord-333636-h2sg6shp	285	2	vivo	vivo	NN
cord-333636-h2sg6shp	285	3	detection	detection	NN
cord-333636-h2sg6shp	285	4	,	,	,
cord-333636-h2sg6shp	285	5	RNAbinding	rnabinding	NN
cord-333636-h2sg6shp	285	6	properties	property	NNS
cord-333636-h2sg6shp	285	7	and	and	CC
cord-333636-h2sg6shp	285	8	characterization	characterization	NN
cord-333636-h2sg6shp	285	9	of	of	IN
cord-333636-h2sg6shp	285	10	the	the	DT
cord-333636-h2sg6shp	285	11	RNA	RNA	NNP
cord-333636-h2sg6shp	285	12	-	-	HYPH
cord-333636-h2sg6shp	285	13	binding	bind	VBG
cord-333636-h2sg6shp	285	14	domain	domain	NN
cord-333636-h2sg6shp	285	15	of	of	IN
cord-333636-h2sg6shp	285	16	the	the	DT
cord-333636-h2sg6shp	285	17	p7	p7	NNP
cord-333636-h2sg6shp	285	18	putative	putative	JJ
cord-333636-h2sg6shp	285	19	movement	movement	NN
cord-333636-h2sg6shp	285	20	protein	protein	NN
cord-333636-h2sg6shp	285	21	from	from	IN
cord-333636-h2sg6shp	285	22	carnation	carnation	NNP
cord-333636-h2sg6shp	285	23	mottle	mottle	NN
cord-333636-h2sg6shp	285	24	carmovirus	carmovirus	NNP
cord-333636-h2sg6shp	285	25	(	(	-LRB-
cord-333636-h2sg6shp	285	26	CarMV	CarMV	NNP
cord-333636-h2sg6shp	285	27	)	)	-RRB-
cord-333636-h2sg6shp	286	1	The	the	DT
cord-333636-h2sg6shp	286	2	two	two	CD
cord-333636-h2sg6shp	286	3	segments	segment	NNS
cord-333636-h2sg6shp	286	4	of	of	IN
cord-333636-h2sg6shp	286	5	the	the	DT
cord-333636-h2sg6shp	286	6	infectious	infectious	JJ
cord-333636-h2sg6shp	286	7	bursal	bursal	NNP
cord-333636-h2sg6shp	286	8	disease	disease	NNP
cord-333636-h2sg6shp	286	9	virus	virus	NN
cord-333636-h2sg6shp	286	10	genome	genome	NN
cord-333636-h2sg6shp	286	11	are	be	VBP
cord-333636-h2sg6shp	286	12	circularized	circularize	VBN
cord-333636-h2sg6shp	286	13	by	by	IN
cord-333636-h2sg6shp	286	14	a	a	DT
cord-333636-h2sg6shp	286	15	90,000-Da	90,000-da	CD
cord-333636-h2sg6shp	286	16	protein	protein	NN
cord-333636-h2sg6shp	286	17	RNA	RNA	NNP
cord-333636-h2sg6shp	286	18	is	be	VBZ
cord-333636-h2sg6shp	286	19	a	a	DT
cord-333636-h2sg6shp	286	20	structural	structural	JJ
cord-333636-h2sg6shp	286	21	element	element	NN
cord-333636-h2sg6shp	286	22	in	in	IN
cord-333636-h2sg6shp	286	23	retrovirus	retrovirus	NN
cord-333636-h2sg6shp	286	24	particles	particle	VBZ
cord-333636-h2sg6shp	286	25	Infectious	infectious	JJ
cord-333636-h2sg6shp	286	26	bursal	bursal	NN
cord-333636-h2sg6shp	286	27	disease	disease	NN
cord-333636-h2sg6shp	286	28	virus	virus	NN
cord-333636-h2sg6shp	286	29	:	:	:
cord-333636-h2sg6shp	286	30	a	a	DT
cord-333636-h2sg6shp	286	31	review	review	NN
cord-333636-h2sg6shp	286	32	of	of	IN
cord-333636-h2sg6shp	286	33	molecular	molecular	JJ
cord-333636-h2sg6shp	286	34	basis	basis	NN
cord-333636-h2sg6shp	286	35	for	for	IN
cord-333636-h2sg6shp	286	36	variations	variation	NNS
cord-333636-h2sg6shp	286	37	in	in	IN
cord-333636-h2sg6shp	286	38	antigenicity	antigenicity	NN
cord-333636-h2sg6shp	286	39	and	and	CC
cord-333636-h2sg6shp	286	40	virulence	virulence	NN
cord-333636-h2sg6shp	286	41	Seroprevalence	Seroprevalence	NNP
cord-333636-h2sg6shp	286	42	of	of	IN
cord-333636-h2sg6shp	286	43	infectious	infectious	JJ
cord-333636-h2sg6shp	286	44	bursal	bursal	NN
cord-333636-h2sg6shp	286	45	disease	disease	NN
cord-333636-h2sg6shp	286	46	virus	virus	NN
cord-333636-h2sg6shp	286	47	in	in	IN
cord-333636-h2sg6shp	286	48	free	free	RB
cord-333636-h2sg6shp	286	49	-	-	HYPH
cord-333636-h2sg6shp	286	50	living	live	VBG
cord-333636-h2sg6shp	286	51	wild	wild	JJ
cord-333636-h2sg6shp	286	52	birds	bird	NNS
cord-333636-h2sg6shp	286	53	in	in	IN
cord-333636-h2sg6shp	286	54	Japan	Japan	NNP
cord-333636-h2sg6shp	287	1	In	in	IN
cord-333636-h2sg6shp	287	2	vitro	vitro	FW
cord-333636-h2sg6shp	287	3	recombination	recombination	NN
cord-333636-h2sg6shp	287	4	and	and	CC
cord-333636-h2sg6shp	287	5	mutagenesis	mutagenesis	NN
cord-333636-h2sg6shp	287	6	by	by	IN
cord-333636-h2sg6shp	287	7	overlap	overlap	NN
cord-333636-h2sg6shp	287	8	extension	extension	NN
cord-333636-h2sg6shp	287	9	PCR	PCR	NNP
cord-333636-h2sg6shp	287	10	Linear	Linear	NNP
cord-333636-h2sg6shp	287	11	correlation	correlation	NN
cord-333636-h2sg6shp	288	1	A	a	DT
cord-333636-h2sg6shp	288	2	major	major	JJ
cord-333636-h2sg6shp	288	3	structural	structural	JJ
cord-333636-h2sg6shp	288	4	protein	protein	NN
cord-333636-h2sg6shp	288	5	of	of	IN
cord-333636-h2sg6shp	288	6	IBDV	IBDV	NNP
cord-333636-h2sg6shp	288	7	Sequence	sequence	NN
cord-333636-h2sg6shp	288	8	-	-	HYPH
cord-333636-h2sg6shp	288	9	specific	specific	JJ
cord-333636-h2sg6shp	288	10	interaction	interaction	NN
cord-333636-h2sg6shp	288	11	between	between	IN
cord-333636-h2sg6shp	288	12	HIV-1	HIV-1	NNP
cord-333636-h2sg6shp	288	13	matrix	matrix	NN
cord-333636-h2sg6shp	288	14	protein	protein	NN
cord-333636-h2sg6shp	288	15	and	and	CC
cord-333636-h2sg6shp	288	16	viral	viral	JJ
cord-333636-h2sg6shp	288	17	genomic	genomic	JJ
cord-333636-h2sg6shp	288	18	RNA	rna	NN
cord-333636-h2sg6shp	288	19	revealed	reveal	VBN
cord-333636-h2sg6shp	288	20	by	by	IN
cord-333636-h2sg6shp	288	21	in	in	IN
cord-333636-h2sg6shp	288	22	vitro	vitro	FW
cord-333636-h2sg6shp	288	23	genetic	genetic	JJ
cord-333636-h2sg6shp	288	24	selection	selection	NN
cord-333636-h2sg6shp	288	25	Infectious	infectious	JJ
cord-333636-h2sg6shp	288	26	bursal	bursal	NN
cord-333636-h2sg6shp	288	27	disease	disease	NN
cord-333636-h2sg6shp	288	28	and	and	CC
cord-333636-h2sg6shp	288	29	hemorrhagic	hemorrhagic	JJ
cord-333636-h2sg6shp	288	30	enteritis	enteritis	NN
cord-333636-h2sg6shp	288	31	Proteolytic	Proteolytic	NNP
cord-333636-h2sg6shp	288	32	processing	processing	NN
cord-333636-h2sg6shp	288	33	in	in	IN
cord-333636-h2sg6shp	288	34	infectious	infectious	JJ
cord-333636-h2sg6shp	288	35	bursal	bursal	NN
cord-333636-h2sg6shp	288	36	disease	disease	NN
cord-333636-h2sg6shp	288	37	virus	virus	NN
cord-333636-h2sg6shp	288	38	:	:	:
cord-333636-h2sg6shp	288	39	identification	identification	NN
cord-333636-h2sg6shp	288	40	of	of	IN
cord-333636-h2sg6shp	288	41	the	the	DT
cord-333636-h2sg6shp	288	42	polyprotein	polyprotein	JJ
cord-333636-h2sg6shp	288	43	cleavage	cleavage	NN
cord-333636-h2sg6shp	288	44	sites	site	NNS
cord-333636-h2sg6shp	288	45	by	by	IN
cord-333636-h2sg6shp	288	46	site	site	NN
cord-333636-h2sg6shp	288	47	-	-	HYPH
cord-333636-h2sg6shp	288	48	directed	direct	VBN
cord-333636-h2sg6shp	288	49	mutagenesis	mutagenesis	NN
cord-333636-h2sg6shp	288	50	Bioinformatics	Bioinformatics	NNPS
cord-333636-h2sg6shp	288	51	:	:	:
cord-333636-h2sg6shp	288	52	a	a	DT
cord-333636-h2sg6shp	288	53	practical	practical	JJ
cord-333636-h2sg6shp	288	54	guide	guide	NN
cord-333636-h2sg6shp	288	55	to	to	IN
cord-333636-h2sg6shp	288	56	the	the	DT
cord-333636-h2sg6shp	288	57	analysis	analysis	NN
cord-333636-h2sg6shp	288	58	of	of	IN
cord-333636-h2sg6shp	288	59	genes	gene	NNS
cord-333636-h2sg6shp	288	60	and	and	CC
cord-333636-h2sg6shp	288	61	proteins	protein	NNS
cord-333636-h2sg6shp	288	62	Quantitative	quantitative	JJ
cord-333636-h2sg6shp	288	63	analysis	analysis	NN
cord-333636-h2sg6shp	288	64	of	of	IN
cord-333636-h2sg6shp	288	65	the	the	DT
cord-333636-h2sg6shp	288	66	binding	binding	NN
cord-333636-h2sg6shp	288	67	of	of	IN
cord-333636-h2sg6shp	288	68	turnip	turnip	NN
cord-333636-h2sg6shp	288	69	crinkle	crinkle	NN
cord-333636-h2sg6shp	288	70	virus	virus	NN
cord-333636-h2sg6shp	288	71	coat	coat	NN
cord-333636-h2sg6shp	288	72	protein	protein	NN
cord-333636-h2sg6shp	288	73	to	to	IN
cord-333636-h2sg6shp	288	74	RNA	RNA	NNP
cord-333636-h2sg6shp	288	75	fails	fail	VBZ
cord-333636-h2sg6shp	288	76	to	to	TO
cord-333636-h2sg6shp	288	77	demonstrate	demonstrate	VB
cord-333636-h2sg6shp	288	78	binding	bind	VBG
cord-333636-h2sg6shp	288	79	specificity	specificity	NN
cord-333636-h2sg6shp	288	80	but	but	CC
cord-333636-h2sg6shp	288	81	reveals	reveal	VBZ
cord-333636-h2sg6shp	288	82	a	a	DT
cord-333636-h2sg6shp	288	83	highly	highly	RB
cord-333636-h2sg6shp	288	84	cooperative	cooperative	JJ
cord-333636-h2sg6shp	288	85	assembly	assembly	NN
cord-333636-h2sg6shp	288	86	interaction	interaction	NN
cord-333636-h2sg6shp	288	87	Interactions	Interactions	NNPS
cord-333636-h2sg6shp	288	88	in	in	IN
cord-333636-h2sg6shp	288	89	vivo	vivo	NN
cord-333636-h2sg6shp	288	90	between	between	IN
cord-333636-h2sg6shp	288	91	the	the	DT
cord-333636-h2sg6shp	288	92	proteins	protein	NNS
cord-333636-h2sg6shp	288	93	of	of	IN
cord-333636-h2sg6shp	288	94	infectious	infectious	JJ
cord-333636-h2sg6shp	288	95	bursal	bursal	NN
cord-333636-h2sg6shp	288	96	disease	disease	NN
cord-333636-h2sg6shp	288	97	virus	virus	NN
cord-333636-h2sg6shp	288	98	:	:	:
cord-333636-h2sg6shp	289	1	capsid	capsid	NN
cord-333636-h2sg6shp	289	2	protein	protein	NN
cord-333636-h2sg6shp	289	3	VP3	VP3	NNP
cord-333636-h2sg6shp	289	4	interacts	interact	VBZ
cord-333636-h2sg6shp	289	5	with	with	IN
cord-333636-h2sg6shp	289	6	the	the	DT
cord-333636-h2sg6shp	289	7	RNA	RNA	NNP
cord-333636-h2sg6shp	289	8	-	-	HYPH
cord-333636-h2sg6shp	289	9	dependent	dependent	JJ
cord-333636-h2sg6shp	289	10	RNA	RNA	NNP
cord-333636-h2sg6shp	289	11	polymerase	polymerase	NN
cord-333636-h2sg6shp	289	12	,	,	,
cord-333636-h2sg6shp	289	13	VP1	VP1	NNP
cord-333636-h2sg6shp	289	14	Stringent	stringent	JJ
cord-333636-h2sg6shp	289	15	chemical	chemical	NN
cord-333636-h2sg6shp	289	16	and	and	CC
cord-333636-h2sg6shp	289	17	thermal	thermal	JJ
cord-333636-h2sg6shp	289	18	regulation	regulation	NN
cord-333636-h2sg6shp	289	19	of	of	IN
cord-333636-h2sg6shp	289	20	recombinant	recombinant	JJ
cord-333636-h2sg6shp	289	21	gene	gene	NN
cord-333636-h2sg6shp	289	22	expression	expression	NN
cord-333636-h2sg6shp	289	23	by	by	IN
cord-333636-h2sg6shp	289	24	vaccinia	vaccinia	NN
cord-333636-h2sg6shp	289	25	virus	virus	NN
cord-333636-h2sg6shp	289	26	vectors	vector	NNS
cord-333636-h2sg6shp	289	27	in	in	IN
cord-333636-h2sg6shp	289	28	mammalian	mammalian	JJ
cord-333636-h2sg6shp	289	29	cells	cell	NNS
cord-333636-h2sg6shp	289	30	Infectious	infectious	JJ
cord-333636-h2sg6shp	289	31	bursal	bursal	NN
cord-333636-h2sg6shp	289	32	disease	disease	NN
cord-333636-h2sg6shp	289	33	in	in	IN
cord-333636-h2sg6shp	289	34	Western	Western	NNP
cord-333636-h2sg6shp	289	35	Australia	Australia	NNP
cord-333636-h2sg6shp	289	36	Induction	Induction	NNP
cord-333636-h2sg6shp	289	37	of	of	IN
cord-333636-h2sg6shp	289	38	apoptosis	apoptosis	NN
cord-333636-h2sg6shp	289	39	in	in	IN
cord-333636-h2sg6shp	289	40	vitro	vitro	FW
cord-333636-h2sg6shp	289	41	by	by	IN
cord-333636-h2sg6shp	289	42	the	the	DT
cord-333636-h2sg6shp	289	43	17-kDa	17-kda	CD
cord-333636-h2sg6shp	289	44	nonstructural	nonstructural	JJ
cord-333636-h2sg6shp	289	45	protein	protein	NN
cord-333636-h2sg6shp	289	46	of	of	IN
cord-333636-h2sg6shp	289	47	infectious	infectious	JJ
cord-333636-h2sg6shp	289	48	bursal	bursal	NNP
cord-333636-h2sg6shp	289	49	disease	disease	NN
cord-333636-h2sg6shp	289	50	virus	virus	NN
cord-333636-h2sg6shp	289	51	:	:	:
cord-333636-h2sg6shp	289	52	possible	possible	JJ
cord-333636-h2sg6shp	289	53	role	role	NN
cord-333636-h2sg6shp	289	54	in	in	IN
cord-333636-h2sg6shp	289	55	viral	viral	JJ
cord-333636-h2sg6shp	289	56	pathogenesis	pathogenesis	NN
cord-333636-h2sg6shp	290	1	We	-PRON-	PRP
cord-333636-h2sg6shp	290	2	are	be	VBP
cord-333636-h2sg6shp	290	3	grateful	grateful	JJ
cord-333636-h2sg6shp	290	4	to	to	IN
cord-333636-h2sg6shp	290	5	J.	J.	NNP
cord-333636-h2sg6shp	290	6	Castón	Castón	NNP
cord-333636-h2sg6shp	290	7	and	and	CC
cord-333636-h2sg6shp	290	8	A.	A.	NNP
cord-333636-h2sg6shp	290	9	Maraver	Maraver	NNP
cord-333636-h2sg6shp	290	10	for	for	IN
cord-333636-h2sg6shp	290	11	critical	critical	JJ
cord-333636-h2sg6shp	290	12	reading	reading	NN
cord-333636-h2sg6shp	290	13	of	of	IN
cord-333636-h2sg6shp	290	14	the	the	DT
cord-333636-h2sg6shp	290	15	manuscript	manuscript	NN
cord-333636-h2sg6shp	290	16	;	;	:
cord-333636-h2sg6shp	290	17	to	to	IN
cord-333636-h2sg6shp	290	18	S.	S.	NNP
cord-333636-h2sg6shp	290	19	Gonzalez	Gonzalez	NNP
cord-333636-h2sg6shp	290	20	and	and	CC
cord-333636-h2sg6shp	290	21	P.	P.	NNP
cord-333636-h2sg6shp	290	22	Gastaminza	Gastaminza	NNP
cord-333636-h2sg6shp	290	23	for	for	IN
cord-333636-h2sg6shp	290	24	interesting	interesting	JJ
cord-333636-h2sg6shp	290	25	and	and	CC
cord-333636-h2sg6shp	290	26	helpful	helpful	JJ
cord-333636-h2sg6shp	290	27	discussion	discussion	NN
cord-333636-h2sg6shp	290	28	;	;	:
cord-333636-h2sg6shp	290	29	to	to	IN
cord-333636-h2sg6shp	290	30	H.	H.	NNP
cord-333636-h2sg6shp	290	31	Müller	Müller	NNP
cord-333636-h2sg6shp	290	32	for	for	IN
cord-333636-h2sg6shp	290	33	anti	anti	JJ
cord-333636-h2sg6shp	290	34	-	-	JJ
cord-333636-h2sg6shp	290	35	VP3	vp3	JJ
cord-333636-h2sg6shp	290	36	monoclonal	monoclonal	JJ
cord-333636-h2sg6shp	290	37	antibodies	antibody	NNS
cord-333636-h2sg6shp	290	38	;	;	:
cord-333636-h2sg6shp	290	39	A.	A.	NNP
cord-333636-h2sg6shp	290	40	Maraver	Maraver	NNP
cord-333636-h2sg6shp	290	41	for	for	IN
cord-333636-h2sg6shp	290	42	plasmid	plasmid	NN
cord-333636-h2sg6shp	291	1	TV2	TV2	NNP
cord-333636-h2sg6shp	291	2	-	-	HYPH
cord-333636-h2sg6shp	291	3	5	5	CD
cord-333636-h2sg6shp	291	4	3	3	CD
cord-333636-h2sg6shp	291	5	;	;	:
cord-333636-h2sg6shp	291	6	to	to	IN
cord-333636-h2sg6shp	291	7	E.	E.	NNP
cord-333636-h2sg6shp	291	8	Camafeita	Camafeita	NNP
cord-333636-h2sg6shp	291	9	for	for	IN
cord-333636-h2sg6shp	291	10	protein	protein	NN
cord-333636-h2sg6shp	291	11	analyses	analysis	NNS
cord-333636-h2sg6shp	291	12	.	.	.
cord-333636-h2sg6shp	292	1	This	this	DT
cord-333636-h2sg6shp	292	2	work	work	NN
cord-333636-h2sg6shp	292	3	was	be	VBD
cord-333636-h2sg6shp	292	4	supported	support	VBN
cord-333636-h2sg6shp	292	5	by	by	IN
cord-333636-h2sg6shp	292	6	grants	grant	NNS
cord-333636-h2sg6shp	292	7	BIO-97	BIO-97	NNP
cord-333636-h2sg6shp	292	8	-	-	HYPH
cord-333636-h2sg6shp	292	9	0576	0576	CD
cord-333636-h2sg6shp	292	10	from	from	IN
cord-333636-h2sg6shp	292	11	the	the	DT
cord-333636-h2sg6shp	292	12	Comisión	Comisión	NNP
cord-333636-h2sg6shp	292	13	Interministerial	Interministerial	NNP
cord-333636-h2sg6shp	292	14	de	de	FW
cord-333636-h2sg6shp	292	15	Ciencia	Ciencia	NNP
cord-333636-h2sg6shp	292	16	y	y	NNP
cord-333636-h2sg6shp	292	17	Tecnología	Tecnología	NNP
cord-333636-h2sg6shp	292	18	,	,	,
cord-333636-h2sg6shp	292	19	07B/0032/1998	07b/0032/1998	CD
cord-333636-h2sg6shp	292	20	from	from	IN
cord-333636-h2sg6shp	292	21	the	the	DT
cord-333636-h2sg6shp	292	22	Subdirección	Subdirección	NNP
cord-333636-h2sg6shp	292	23	General	General	NNP
cord-333636-h2sg6shp	292	24	de	de	NNP
cord-333636-h2sg6shp	292	25	Investigación	Investigación	NNP
cord-333636-h2sg6shp	292	26	of	of	IN
cord-333636-h2sg6shp	292	27	the	the	DT
cord-333636-h2sg6shp	292	28	Comunidad	Comunidad	NNP
cord-333636-h2sg6shp	292	29	Autónoma	Autónoma	NNP
cord-333636-h2sg6shp	292	30	de	de	NNP
cord-333636-h2sg6shp	292	31	Madrid	Madrid	NNP
cord-333636-h2sg6shp	292	32	.	.	.