id sid tid token lemma pos cord-341342-kyavg4vu 1 1 key key JJ cord-341342-kyavg4vu 1 2 : : : cord-341342-kyavg4vu 1 3 cord-341342-kyavg4vu cord-341342-kyavg4vu JJS cord-341342-kyavg4vu 1 4 authors author NNS cord-341342-kyavg4vu 1 5 : : : cord-341342-kyavg4vu 1 6 Masters Masters NNP cord-341342-kyavg4vu 1 7 , , , cord-341342-kyavg4vu 1 8 P. P. NNP cord-341342-kyavg4vu 1 9 S. S. NNP cord-341342-kyavg4vu 2 1 title title NN cord-341342-kyavg4vu 2 2 : : : cord-341342-kyavg4vu 2 3 Localization localization NN cord-341342-kyavg4vu 2 4 of of IN cord-341342-kyavg4vu 2 5 an an DT cord-341342-kyavg4vu 2 6 RNA rna NN cord-341342-kyavg4vu 2 7 - - HYPH cord-341342-kyavg4vu 2 8 binding bind VBG cord-341342-kyavg4vu 2 9 domain domain NN cord-341342-kyavg4vu 2 10 in in IN cord-341342-kyavg4vu 2 11 the the DT cord-341342-kyavg4vu 2 12 nucleocapsid nucleocapsid NNP cord-341342-kyavg4vu 2 13 protein protein NN cord-341342-kyavg4vu 2 14 of of IN cord-341342-kyavg4vu 2 15 the the DT cord-341342-kyavg4vu 2 16 coronavirus coronavirus NN cord-341342-kyavg4vu 2 17 mouse mouse NN cord-341342-kyavg4vu 2 18 hepatitis hepatitis NN cord-341342-kyavg4vu 2 19 virus virus NN cord-341342-kyavg4vu 2 20 date date NN cord-341342-kyavg4vu 2 21 : : : cord-341342-kyavg4vu 2 22 1992 1992 CD cord-341342-kyavg4vu 2 23 journal journal NN cord-341342-kyavg4vu 2 24 : : : cord-341342-kyavg4vu 3 1 Arch arch JJ cord-341342-kyavg4vu 3 2 Virol Virol NNP cord-341342-kyavg4vu 3 3 DOI doi NN cord-341342-kyavg4vu 3 4 : : : cord-341342-kyavg4vu 4 1 10.1007 10.1007 CD cord-341342-kyavg4vu 4 2 / / SYM cord-341342-kyavg4vu 4 3 bf01309634 bf01309634 NNP cord-341342-kyavg4vu 4 4 sha sha NNP cord-341342-kyavg4vu 4 5 : : : cord-341342-kyavg4vu 4 6 914c0d5e58d5c2aceedb6ab07c555e1aaba6018d 914c0d5e58d5c2aceedb6ab07c555e1aaba6018d NNP cord-341342-kyavg4vu 4 7 doc_id doc_id CD cord-341342-kyavg4vu 4 8 : : : cord-341342-kyavg4vu 4 9 341342 341342 CD cord-341342-kyavg4vu 4 10 cord_uid cord_uid NNS cord-341342-kyavg4vu 4 11 : : : cord-341342-kyavg4vu 5 1 kyavg4vu kyavg4vu NNP cord-341342-kyavg4vu 6 1 The the DT cord-341342-kyavg4vu 6 2 interaction interaction NN cord-341342-kyavg4vu 6 3 between between IN cord-341342-kyavg4vu 6 4 the the DT cord-341342-kyavg4vu 6 5 nucleocapsid nucleocapsid NN cord-341342-kyavg4vu 6 6 ( ( -LRB- cord-341342-kyavg4vu 6 7 N N NNP cord-341342-kyavg4vu 6 8 ) ) -RRB- cord-341342-kyavg4vu 6 9 protein protein NN cord-341342-kyavg4vu 6 10 of of IN cord-341342-kyavg4vu 6 11 mouse mouse NNP cord-341342-kyavg4vu 6 12 hepatitis hepatitis NNP cord-341342-kyavg4vu 6 13 virus virus NN cord-341342-kyavg4vu 6 14 ( ( -LRB- cord-341342-kyavg4vu 6 15 MHV MHV NNP cord-341342-kyavg4vu 6 16 ) ) -RRB- cord-341342-kyavg4vu 6 17 and and CC cord-341342-kyavg4vu 6 18 RNA RNA NNP cord-341342-kyavg4vu 6 19 was be VBD cord-341342-kyavg4vu 6 20 studied study VBN cord-341342-kyavg4vu 6 21 in in IN cord-341342-kyavg4vu 6 22 an an DT cord-341342-kyavg4vu 6 23 effort effort NN cord-341342-kyavg4vu 6 24 to to TO cord-341342-kyavg4vu 6 25 define define VB cord-341342-kyavg4vu 6 26 portions portion NNS cord-341342-kyavg4vu 6 27 of of IN cord-341342-kyavg4vu 6 28 the the DT cord-341342-kyavg4vu 6 29 N n CD cord-341342-kyavg4vu 6 30 molecule molecule NN cord-341342-kyavg4vu 6 31 that that WDT cord-341342-kyavg4vu 6 32 participate participate VBP cord-341342-kyavg4vu 6 33 in in IN cord-341342-kyavg4vu 6 34 binding bind VBG cord-341342-kyavg4vu 6 35 to to IN cord-341342-kyavg4vu 6 36 RNA RNA NNP cord-341342-kyavg4vu 6 37 . . . cord-341342-kyavg4vu 7 1 N N NNP cord-341342-kyavg4vu 7 2 mRNAs mrnas PRP cord-341342-kyavg4vu 7 3 transcribed transcribe VBN cord-341342-kyavg4vu 7 4 from from IN cord-341342-kyavg4vu 7 5 SP6 SP6 NNP cord-341342-kyavg4vu 7 6 and and CC cord-341342-kyavg4vu 7 7 T7 t7 NN cord-341342-kyavg4vu 7 8 vectors vector NNS cord-341342-kyavg4vu 7 9 were be VBD cord-341342-kyavg4vu 7 10 translated translate VBN cord-341342-kyavg4vu 7 11 in in IN cord-341342-kyavg4vu 7 12 a a DT cord-341342-kyavg4vu 7 13 rabbit rabbit NN cord-341342-kyavg4vu 7 14 reticulocyte reticulocyte NN cord-341342-kyavg4vu 7 15 lysate lysate NN cord-341342-kyavg4vu 7 16 . . . cord-341342-kyavg4vu 8 1 Analysis analysis NN cord-341342-kyavg4vu 8 2 of of IN cord-341342-kyavg4vu 8 3 synthesized synthesize VBN cord-341342-kyavg4vu 8 4 N n CD cord-341342-kyavg4vu 8 5 protein protein NN cord-341342-kyavg4vu 8 6 in in IN cord-341342-kyavg4vu 8 7 a a DT cord-341342-kyavg4vu 8 8 nondenaturing nondenature VBG cord-341342-kyavg4vu 8 9 gel gel NN cord-341342-kyavg4vu 8 10 system system NN cord-341342-kyavg4vu 8 11 showed show VBD cord-341342-kyavg4vu 8 12 that that IN cord-341342-kyavg4vu 8 13 it -PRON- PRP cord-341342-kyavg4vu 8 14 bound bind VBN cord-341342-kyavg4vu 8 15 in in IN cord-341342-kyavg4vu 8 16 vitro vitro FW cord-341342-kyavg4vu 8 17 to to IN cord-341342-kyavg4vu 8 18 an an DT cord-341342-kyavg4vu 8 19 endogenous endogenous JJ cord-341342-kyavg4vu 8 20 RNA RNA NNP cord-341342-kyavg4vu 8 21 in in IN cord-341342-kyavg4vu 8 22 the the DT cord-341342-kyavg4vu 8 23 reticulocyte reticulocyte NN cord-341342-kyavg4vu 8 24 lysate lysate NN cord-341342-kyavg4vu 8 25 but but CC cord-341342-kyavg4vu 8 26 not not RB cord-341342-kyavg4vu 8 27 to to IN cord-341342-kyavg4vu 8 28 its -PRON- PRP$ cord-341342-kyavg4vu 8 29 own own JJ cord-341342-kyavg4vu 8 30 mRNA mrna NN cord-341342-kyavg4vu 8 31 . . . cord-341342-kyavg4vu 9 1 A a DT cord-341342-kyavg4vu 9 2 set set NN cord-341342-kyavg4vu 9 3 of of IN cord-341342-kyavg4vu 9 4 deletion deletion NN cord-341342-kyavg4vu 9 5 mutants mutant NNS cord-341342-kyavg4vu 9 6 was be VBD cord-341342-kyavg4vu 9 7 constructed construct VBN cord-341342-kyavg4vu 9 8 in in IN cord-341342-kyavg4vu 9 9 order order NN cord-341342-kyavg4vu 9 10 to to TO cord-341342-kyavg4vu 9 11 localize localize VB cord-341342-kyavg4vu 9 12 the the DT cord-341342-kyavg4vu 9 13 RNA rna NN cord-341342-kyavg4vu 9 14 - - HYPH cord-341342-kyavg4vu 9 15 binding bind VBG cord-341342-kyavg4vu 9 16 activity activity NN cord-341342-kyavg4vu 9 17 of of IN cord-341342-kyavg4vu 9 18 the the DT cord-341342-kyavg4vu 9 19 N n CD cord-341342-kyavg4vu 9 20 protein protein NN cord-341342-kyavg4vu 9 21 . . . cord-341342-kyavg4vu 10 1 It -PRON- PRP cord-341342-kyavg4vu 10 2 was be VBD cord-341342-kyavg4vu 10 3 found find VBN cord-341342-kyavg4vu 10 4 that that IN cord-341342-kyavg4vu 10 5 removal removal NN cord-341342-kyavg4vu 10 6 of of IN cord-341342-kyavg4vu 10 7 as as RB cord-341342-kyavg4vu 10 8 much much JJ cord-341342-kyavg4vu 10 9 as as IN cord-341342-kyavg4vu 10 10 135 135 CD cord-341342-kyavg4vu 10 11 amino amino NN cord-341342-kyavg4vu 10 12 - - HYPH cord-341342-kyavg4vu 10 13 terminal terminal NN cord-341342-kyavg4vu 10 14 or or CC cord-341342-kyavg4vu 10 15 57 57 CD cord-341342-kyavg4vu 10 16 carboxy carboxy JJ cord-341342-kyavg4vu 10 17 - - HYPH cord-341342-kyavg4vu 10 18 terminal terminal NN cord-341342-kyavg4vu 10 19 amino amino NN cord-341342-kyavg4vu 10 20 acids acid NNS cord-341342-kyavg4vu 10 21 from from IN cord-341342-kyavg4vu 10 22 the the DT cord-341342-kyavg4vu 10 23 molecule molecule NN cord-341342-kyavg4vu 10 24 had have VBD cord-341342-kyavg4vu 10 25 little little JJ cord-341342-kyavg4vu 10 26 or or CC cord-341342-kyavg4vu 10 27 no no DT cord-341342-kyavg4vu 10 28 effect effect NN cord-341342-kyavg4vu 10 29 on on IN cord-341342-kyavg4vu 10 30 RNA RNA NNP cord-341342-kyavg4vu 10 31 binding binding NN cord-341342-kyavg4vu 10 32 . . . cord-341342-kyavg4vu 11 1 Moreover moreover RB cord-341342-kyavg4vu 11 2 , , , cord-341342-kyavg4vu 11 3 deletion deletion NN cord-341342-kyavg4vu 11 4 mutants mutant NNS cord-341342-kyavg4vu 11 5 lacking lack VBG cord-341342-kyavg4vu 11 6 both both DT cord-341342-kyavg4vu 11 7 termini terminus NNS cord-341342-kyavg4vu 11 8 still still RB cord-341342-kyavg4vu 11 9 retained retain VBD cord-341342-kyavg4vu 11 10 RNA RNA NNP cord-341342-kyavg4vu 11 11 - - HYPH cord-341342-kyavg4vu 11 12 binding bind VBG cord-341342-kyavg4vu 11 13 ability ability NN cord-341342-kyavg4vu 11 14 . . . cord-341342-kyavg4vu 12 1 By by IN cord-341342-kyavg4vu 12 2 contrast contrast NN cord-341342-kyavg4vu 12 3 , , , cord-341342-kyavg4vu 12 4 internal internal JJ cord-341342-kyavg4vu 12 5 deletions deletion NNS cord-341342-kyavg4vu 12 6 or or CC cord-341342-kyavg4vu 12 7 truncations truncation NNS cord-341342-kyavg4vu 12 8 extending extend VBG cord-341342-kyavg4vu 12 9 beyond beyond IN cord-341342-kyavg4vu 12 10 these these DT cord-341342-kyavg4vu 12 11 two two CD cord-341342-kyavg4vu 12 12 limits limit NNS cord-341342-kyavg4vu 12 13 effectively effectively RB cord-341342-kyavg4vu 12 14 abolished abolish VBD cord-341342-kyavg4vu 12 15 RNA RNA NNP cord-341342-kyavg4vu 12 16 binding bind VBG cord-341342-kyavg4vu 12 17 by by IN cord-341342-kyavg4vu 12 18 N n CD cord-341342-kyavg4vu 12 19 protein protein NN cord-341342-kyavg4vu 12 20 . . . cord-341342-kyavg4vu 13 1 Thus thus RB cord-341342-kyavg4vu 13 2 , , , cord-341342-kyavg4vu 13 3 the the DT cord-341342-kyavg4vu 13 4 RNA RNA NNP cord-341342-kyavg4vu 13 5 - - HYPH cord-341342-kyavg4vu 13 6 binding bind VBG cord-341342-kyavg4vu 13 7 region region NN cord-341342-kyavg4vu 13 8 of of IN cord-341342-kyavg4vu 13 9 N N NNP cord-341342-kyavg4vu 13 10 has have VBZ cord-341342-kyavg4vu 13 11 been be VBN cord-341342-kyavg4vu 13 12 mapped map VBN cord-341342-kyavg4vu 13 13 to to IN cord-341342-kyavg4vu 13 14 the the DT cord-341342-kyavg4vu 13 15 second second JJ cord-341342-kyavg4vu 13 16 ( ( -LRB- cord-341342-kyavg4vu 13 17 central central JJ cord-341342-kyavg4vu 13 18 ) ) -RRB- cord-341342-kyavg4vu 13 19 of of IN cord-341342-kyavg4vu 13 20 the the DT cord-341342-kyavg4vu 13 21 three three CD cord-341342-kyavg4vu 13 22 structural structural JJ cord-341342-kyavg4vu 13 23 domains domain NNS cord-341342-kyavg4vu 13 24 of of IN cord-341342-kyavg4vu 13 25 the the DT cord-341342-kyavg4vu 13 26 molecule molecule NN cord-341342-kyavg4vu 13 27 . . . cord-341342-kyavg4vu 14 1 Coronaviruses coronaviruse NNS cord-341342-kyavg4vu 14 2 constitute constitute VBP cord-341342-kyavg4vu 14 3 a a DT cord-341342-kyavg4vu 14 4 family family NN cord-341342-kyavg4vu 14 5 of of IN cord-341342-kyavg4vu 14 6 viruses virus NNS cord-341342-kyavg4vu 15 1 whose whose WP$ cord-341342-kyavg4vu 15 2 single single RB cord-341342-kyavg4vu 15 3 - - HYPH cord-341342-kyavg4vu 15 4 stranded stranded JJ cord-341342-kyavg4vu 15 5 , , , cord-341342-kyavg4vu 15 6 positivesense positivesense NN cord-341342-kyavg4vu 15 7 RNA RNA NNP cord-341342-kyavg4vu 15 8 genomes genome NNS cord-341342-kyavg4vu 15 9 have have VBP cord-341342-kyavg4vu 15 10 the the DT cord-341342-kyavg4vu 15 11 largest large JJS cord-341342-kyavg4vu 15 12 coding code VBG cord-341342-kyavg4vu 15 13 capacities capacity NNS cord-341342-kyavg4vu 15 14 among among IN cord-341342-kyavg4vu 15 15 the the DT cord-341342-kyavg4vu 15 16 RNA RNA NNP cord-341342-kyavg4vu 15 17 viruses virus NNS cord-341342-kyavg4vu 15 18 [ [ -LRB- cord-341342-kyavg4vu 15 19 27 27 CD cord-341342-kyavg4vu 15 20 , , , cord-341342-kyavg4vu 15 21 31 31 CD cord-341342-kyavg4vu 15 22 ] ] -RRB- cord-341342-kyavg4vu 15 23 . . . cord-341342-kyavg4vu 16 1 Paradoxically paradoxically RB cord-341342-kyavg4vu 16 2 , , , cord-341342-kyavg4vu 16 3 coronaviruses coronaviruse NNS cord-341342-kyavg4vu 16 4 have have VBP cord-341342-kyavg4vu 16 5 relatively relatively RB cord-341342-kyavg4vu 16 6 few few JJ cord-341342-kyavg4vu 16 7 structural structural JJ cord-341342-kyavg4vu 16 8 proteins protein NNS cord-341342-kyavg4vu 16 9 . . . cord-341342-kyavg4vu 17 1 Their -PRON- PRP$ cord-341342-kyavg4vu 17 2 large large JJ cord-341342-kyavg4vu 17 3 , , , cord-341342-kyavg4vu 17 4 roughly roughly RB cord-341342-kyavg4vu 17 5 spherical spherical JJ cord-341342-kyavg4vu 17 6 virions virion NNS cord-341342-kyavg4vu 17 7 are be VBP cord-341342-kyavg4vu 17 8 enclosed enclose VBN cord-341342-kyavg4vu 17 9 by by IN cord-341342-kyavg4vu 17 10 a a DT cord-341342-kyavg4vu 17 11 membrane membrane NN cord-341342-kyavg4vu 17 12 envelope envelope NN cord-341342-kyavg4vu 17 13 containing contain VBG cord-341342-kyavg4vu 17 14 two two CD cord-341342-kyavg4vu 17 15 ( ( -LRB- cord-341342-kyavg4vu 17 16 or or CC cord-341342-kyavg4vu 17 17 , , , cord-341342-kyavg4vu 17 18 in in IN cord-341342-kyavg4vu 17 19 some some DT cord-341342-kyavg4vu 17 20 cases case NNS cord-341342-kyavg4vu 17 21 , , , cord-341342-kyavg4vu 17 22 three three CD cord-341342-kyavg4vu 17 23 ) ) -RRB- cord-341342-kyavg4vu 17 24 types type NNS cord-341342-kyavg4vu 17 25 of of IN cord-341342-kyavg4vu 17 26 membrane membrane NN cord-341342-kyavg4vu 17 27 - - HYPH cord-341342-kyavg4vu 17 28 bound bind VBN cord-341342-kyavg4vu 17 29 glycoproteins glycoprotein NNS cord-341342-kyavg4vu 17 30 . . . cord-341342-kyavg4vu 18 1 Internal internal JJ cord-341342-kyavg4vu 18 2 to to IN cord-341342-kyavg4vu 18 3 this this DT cord-341342-kyavg4vu 18 4 , , , cord-341342-kyavg4vu 18 5 multiple multiple JJ cord-341342-kyavg4vu 18 6 monomers monomer NNS cord-341342-kyavg4vu 18 7 of of IN cord-341342-kyavg4vu 18 8 a a DT cord-341342-kyavg4vu 18 9 nucleocapsid nucleocapsid NN cord-341342-kyavg4vu 18 10 protein protein NN cord-341342-kyavg4vu 18 11 ( ( -LRB- cord-341342-kyavg4vu 18 12 N N NNP cord-341342-kyavg4vu 18 13 ) ) -RRB- cord-341342-kyavg4vu 18 14 encapsidate encapsidate VBP cord-341342-kyavg4vu 18 15 the the DT cord-341342-kyavg4vu 18 16 viral viral JJ cord-341342-kyavg4vu 18 17 genome genome NN cord-341342-kyavg4vu 18 18 , , , cord-341342-kyavg4vu 18 19 which which WDT cord-341342-kyavg4vu 18 20 , , , cord-341342-kyavg4vu 18 21 in in IN cord-341342-kyavg4vu 18 22 the the DT cord-341342-kyavg4vu 18 23 case case NN cord-341342-kyavg4vu 18 24 of of IN cord-341342-kyavg4vu 18 25 the the DT cord-341342-kyavg4vu 18 26 prototype prototype NN cord-341342-kyavg4vu 18 27 mouse mouse NN cord-341342-kyavg4vu 18 28 hepatitis hepatitis NNP cord-341342-kyavg4vu 18 29 virus virus NN cord-341342-kyavg4vu 18 30 ( ( -LRB- cord-341342-kyavg4vu 18 31 MHV MHV NNP cord-341342-kyavg4vu 18 32 ) ) -RRB- cord-341342-kyavg4vu 18 33 , , , cord-341342-kyavg4vu 18 34 is be VBZ cord-341342-kyavg4vu 18 35 some some DT cord-341342-kyavg4vu 18 36 31,000 31,000 CD cord-341342-kyavg4vu 18 37 nucleotides nucleotide NNS cord-341342-kyavg4vu 18 38 in in IN cord-341342-kyavg4vu 18 39 length length NN cord-341342-kyavg4vu 18 40 [ [ -LRB- cord-341342-kyavg4vu 18 41 10 10 CD cord-341342-kyavg4vu 18 42 , , , cord-341342-kyavg4vu 18 43 19 19 CD cord-341342-kyavg4vu 18 44 ] ] -RRB- cord-341342-kyavg4vu 18 45 . . . cord-341342-kyavg4vu 19 1 The the DT cord-341342-kyavg4vu 19 2 nucleocapsids nucleocapsid NNS cord-341342-kyavg4vu 19 3 of of IN cord-341342-kyavg4vu 19 4 coronaviruses coronaviruse NNS cord-341342-kyavg4vu 19 5 are be VBP cord-341342-kyavg4vu 19 6 helically helically RB cord-341342-kyavg4vu 19 7 symmetric symmetric JJ cord-341342-kyavg4vu 19 8 [ [ -LRB- cord-341342-kyavg4vu 19 9 12 12 CD cord-341342-kyavg4vu 19 10 ] ] -RRB- cord-341342-kyavg4vu 19 11 , , , cord-341342-kyavg4vu 19 12 a a DT cord-341342-kyavg4vu 19 13 feature feature NN cord-341342-kyavg4vu 19 14 seen see VBN cord-341342-kyavg4vu 19 15 in in IN cord-341342-kyavg4vu 19 16 only only RB cord-341342-kyavg4vu 19 17 one one CD cord-341342-kyavg4vu 19 18 other other JJ cord-341342-kyavg4vu 19 19 group group NN cord-341342-kyavg4vu 19 20 of of IN cord-341342-kyavg4vu 19 21 positive positive JJ cord-341342-kyavg4vu 19 22 - - HYPH cord-341342-kyavg4vu 19 23 stranded strand VBN cord-341342-kyavg4vu 19 24 RNA RNA NNP cord-341342-kyavg4vu 19 25 viruses virus NNS cord-341342-kyavg4vu 19 26 , , , cord-341342-kyavg4vu 19 27 those those DT cord-341342-kyavg4vu 19 28 of of IN cord-341342-kyavg4vu 19 29 the the DT cord-341342-kyavg4vu 19 30 proposed propose VBN cord-341342-kyavg4vu 19 31 family family NN cord-341342-kyavg4vu 19 32 Toro Toro NNP cord-341342-kyavg4vu 19 33 - - HYPH cord-341342-kyavg4vu 19 34 viridae viridae NNP cord-341342-kyavg4vu 19 35 [ [ -LRB- cord-341342-kyavg4vu 19 36 25 25 CD cord-341342-kyavg4vu 19 37 ] ] -RRB- cord-341342-kyavg4vu 19 38 . . . cord-341342-kyavg4vu 20 1 Consequently consequently RB cord-341342-kyavg4vu 20 2 , , , cord-341342-kyavg4vu 20 3 these these DT cord-341342-kyavg4vu 20 4 structures structure NNS cord-341342-kyavg4vu 20 5 pose pose VBP cord-341342-kyavg4vu 20 6 interesting interesting JJ cord-341342-kyavg4vu 20 7 problems problem NNS cord-341342-kyavg4vu 20 8 with with IN cord-341342-kyavg4vu 20 9 respect respect NN cord-341342-kyavg4vu 20 10 to to IN cord-341342-kyavg4vu 20 11 genome genome NN cord-341342-kyavg4vu 20 12 translation translation NN cord-341342-kyavg4vu 20 13 as as RB cord-341342-kyavg4vu 20 14 well well RB cord-341342-kyavg4vu 20 15 as as IN cord-341342-kyavg4vu 20 16 in in IN cord-341342-kyavg4vu 20 17 understanding understand VBG cord-341342-kyavg4vu 20 18 the the DT cord-341342-kyavg4vu 20 19 processes process NNS cord-341342-kyavg4vu 20 20 of of IN cord-341342-kyavg4vu 20 21 encapsidation encapsidation NN cord-341342-kyavg4vu 20 22 and and CC cord-341342-kyavg4vu 20 23 assembly assembly NN cord-341342-kyavg4vu 20 24 . . . cord-341342-kyavg4vu 21 1 Elucidation elucidation NN cord-341342-kyavg4vu 21 2 of of IN cord-341342-kyavg4vu 21 3 coronavirus coronavirus NN cord-341342-kyavg4vu 21 4 nucleocapsid nucleocapsid NN cord-341342-kyavg4vu 21 5 structure structure NN cord-341342-kyavg4vu 21 6 and and CC cord-341342-kyavg4vu 21 7 function function NN cord-341342-kyavg4vu 21 8 will will MD cord-341342-kyavg4vu 21 9 require require VB cord-341342-kyavg4vu 21 10 description description NN cord-341342-kyavg4vu 21 11 of of IN cord-341342-kyavg4vu 21 12 the the DT cord-341342-kyavg4vu 21 13 multiple multiple JJ cord-341342-kyavg4vu 21 14 interactions interaction NNS cord-341342-kyavg4vu 21 15 in in IN cord-341342-kyavg4vu 21 16 which which WDT cord-341342-kyavg4vu 21 17 the the DT cord-341342-kyavg4vu 21 18 N N NNP cord-341342-kyavg4vu 21 19 protein protein NN cord-341342-kyavg4vu 21 20 must must MD cord-341342-kyavg4vu 21 21 participate participate VB cord-341342-kyavg4vu 21 22 : : : cord-341342-kyavg4vu 21 23 ( ( -LRB- cord-341342-kyavg4vu 21 24 i i NN cord-341342-kyavg4vu 21 25 ) ) -RRB- cord-341342-kyavg4vu 22 1 the the DT cord-341342-kyavg4vu 22 2 binding binding NN cord-341342-kyavg4vu 22 3 of of IN cord-341342-kyavg4vu 22 4 N n CD cord-341342-kyavg4vu 22 5 protein protein NN cord-341342-kyavg4vu 22 6 along along IN cord-341342-kyavg4vu 22 7 the the DT cord-341342-kyavg4vu 22 8 entire entire JJ cord-341342-kyavg4vu 22 9 length length NN cord-341342-kyavg4vu 22 10 of of IN cord-341342-kyavg4vu 22 11 the the DT cord-341342-kyavg4vu 22 12 RNA RNA NNP cord-341342-kyavg4vu 22 13 genome genome NN cord-341342-kyavg4vu 22 14 ; ; , cord-341342-kyavg4vu 22 15 ( ( -LRB- cord-341342-kyavg4vu 22 16 ii ii NN cord-341342-kyavg4vu 22 17 ) ) -RRB- cord-341342-kyavg4vu 22 18 contacts contact NNS cord-341342-kyavg4vu 22 19 between between IN cord-341342-kyavg4vu 22 20 adjacent adjacent JJ cord-341342-kyavg4vu 22 21 N n CD cord-341342-kyavg4vu 22 22 monomers monomer NNS cord-341342-kyavg4vu 22 23 on on IN cord-341342-kyavg4vu 22 24 the the DT cord-341342-kyavg4vu 22 25 R r NN cord-341342-kyavg4vu 22 26 N N NNP cord-341342-kyavg4vu 22 27 A A NNP cord-341342-kyavg4vu 22 28 and and CC cord-341342-kyavg4vu 22 29 between between IN cord-341342-kyavg4vu 22 30 N n CD cord-341342-kyavg4vu 22 31 monomers monomer NNS cord-341342-kyavg4vu 22 32 that that WDT cord-341342-kyavg4vu 22 33 neighbor neighbor NN cord-341342-kyavg4vu 22 34 each each DT cord-341342-kyavg4vu 22 35 other other JJ cord-341342-kyavg4vu 22 36 per per IN cord-341342-kyavg4vu 22 37 helical helical JJ cord-341342-kyavg4vu 22 38 turn turn NN cord-341342-kyavg4vu 22 39 ; ; : cord-341342-kyavg4vu 22 40 and and CC cord-341342-kyavg4vu 22 41 ( ( -LRB- cord-341342-kyavg4vu 22 42 iii iii NNP cord-341342-kyavg4vu 22 43 ) ) -RRB- cord-341342-kyavg4vu 22 44 association association NN cord-341342-kyavg4vu 22 45 between between IN cord-341342-kyavg4vu 22 46 N N NNP cord-341342-kyavg4vu 22 47 protein protein NN cord-341342-kyavg4vu 22 48 and and CC cord-341342-kyavg4vu 22 49 the the DT cord-341342-kyavg4vu 22 50 membrane membrane NN cord-341342-kyavg4vu 22 51 glycoprotein glycoprotein NN cord-341342-kyavg4vu 22 52 ( ( -LRB- cord-341342-kyavg4vu 22 53 M M NNP cord-341342-kyavg4vu 22 54 ) ) -RRB- cord-341342-kyavg4vu 22 55 , , , cord-341342-kyavg4vu 22 56 which which WDT cord-341342-kyavg4vu 22 57 may may MD cord-341342-kyavg4vu 22 58 stabilize stabilize VB cord-341342-kyavg4vu 22 59 virion virion NN cord-341342-kyavg4vu 22 60 assembly assembly NN cord-341342-kyavg4vu 22 61 [ [ -LRB- cord-341342-kyavg4vu 22 62 32 32 CD cord-341342-kyavg4vu 22 63 ] ] -RRB- cord-341342-kyavg4vu 22 64 . . . cord-341342-kyavg4vu 23 1 For for IN cord-341342-kyavg4vu 23 2 at at IN cord-341342-kyavg4vu 23 3 least least JJS cord-341342-kyavg4vu 23 4 some some DT cord-341342-kyavg4vu 23 5 of of IN cord-341342-kyavg4vu 23 6 these these DT cord-341342-kyavg4vu 23 7 , , , cord-341342-kyavg4vu 23 8 it -PRON- PRP cord-341342-kyavg4vu 23 9 may may MD cord-341342-kyavg4vu 23 10 be be VB cord-341342-kyavg4vu 23 11 possible possible JJ cord-341342-kyavg4vu 23 12 to to TO cord-341342-kyavg4vu 23 13 map map VB cord-341342-kyavg4vu 23 14 regions region NNS cord-341342-kyavg4vu 23 15 of of IN cord-341342-kyavg4vu 23 16 the the DT cord-341342-kyavg4vu 23 17 N n CD cord-341342-kyavg4vu 23 18 molecule molecule NN cord-341342-kyavg4vu 23 19 that that WDT cord-341342-kyavg4vu 23 20 are be VBP cord-341342-kyavg4vu 23 21 involved involve VBN cord-341342-kyavg4vu 23 22 in in IN cord-341342-kyavg4vu 23 23 a a DT cord-341342-kyavg4vu 23 24 particular particular JJ cord-341342-kyavg4vu 23 25 interaction interaction NN cord-341342-kyavg4vu 23 26 . . . cord-341342-kyavg4vu 24 1 The the DT cord-341342-kyavg4vu 24 2 work work NN cord-341342-kyavg4vu 24 3 presented present VBN cord-341342-kyavg4vu 24 4 here here RB cord-341342-kyavg4vu 24 5 focusses focusse VBZ cord-341342-kyavg4vu 24 6 on on IN cord-341342-kyavg4vu 24 7 the the DT cord-341342-kyavg4vu 24 8 first first JJ cord-341342-kyavg4vu 24 9 type type NN cord-341342-kyavg4vu 24 10 of of IN cord-341342-kyavg4vu 24 11 interaction interaction NN cord-341342-kyavg4vu 24 12 : : : cord-341342-kyavg4vu 24 13 the the DT cord-341342-kyavg4vu 24 14 ability ability NN cord-341342-kyavg4vu 24 15 of of IN cord-341342-kyavg4vu 24 16 N N NNP cord-341342-kyavg4vu 24 17 to to TO cord-341342-kyavg4vu 24 18 bind bind VB cord-341342-kyavg4vu 24 19 to to IN cord-341342-kyavg4vu 24 20 RNA RNA NNP cord-341342-kyavg4vu 24 21 . . . cord-341342-kyavg4vu 25 1 Coronavirus Coronavirus NNP cord-341342-kyavg4vu 25 2 N n CD cord-341342-kyavg4vu 25 3 proteins protein NNS cord-341342-kyavg4vu 25 4 are be VBP cord-341342-kyavg4vu 25 5 markedly markedly RB cord-341342-kyavg4vu 25 6 basic basic JJ cord-341342-kyavg4vu 25 7 , , , cord-341342-kyavg4vu 25 8 and and CC cord-341342-kyavg4vu 25 9 they -PRON- PRP cord-341342-kyavg4vu 25 10 form form VBP cord-341342-kyavg4vu 25 11 complexes complex NNS cord-341342-kyavg4vu 25 12 with with IN cord-341342-kyavg4vu 25 13 RNA RNA NNP cord-341342-kyavg4vu 25 14 that that WDT cord-341342-kyavg4vu 25 15 tend tend VBP cord-341342-kyavg4vu 25 16 to to TO cord-341342-kyavg4vu 25 17 dissociate dissociate VB cord-341342-kyavg4vu 25 18 in in IN cord-341342-kyavg4vu 25 19 high high JJ cord-341342-kyavg4vu 25 20 concentrations concentration NNS cord-341342-kyavg4vu 25 21 of of IN cord-341342-kyavg4vu 25 22 salt salt NN cord-341342-kyavg4vu 25 23 and and CC cord-341342-kyavg4vu 25 24 provide provide VBP cord-341342-kyavg4vu 25 25 only only RB cord-341342-kyavg4vu 25 26 limited limited JJ cord-341342-kyavg4vu 25 27 protection protection NN cord-341342-kyavg4vu 25 28 against against IN cord-341342-kyavg4vu 25 29 the the DT cord-341342-kyavg4vu 25 30 action action NN cord-341342-kyavg4vu 25 31 of of IN cord-341342-kyavg4vu 25 32 ribonucleases ribonuclease NNS cord-341342-kyavg4vu 25 33 [ [ -LRB- cord-341342-kyavg4vu 25 34 12 12 CD cord-341342-kyavg4vu 25 35 , , , cord-341342-kyavg4vu 25 36 22 22 CD cord-341342-kyavg4vu 25 37 ] ] -RRB- cord-341342-kyavg4vu 25 38 . . . cord-341342-kyavg4vu 26 1 In in IN cord-341342-kyavg4vu 26 2 these these DT cord-341342-kyavg4vu 26 3 respects respect NNS cord-341342-kyavg4vu 26 4 , , , cord-341342-kyavg4vu 26 5 they -PRON- PRP cord-341342-kyavg4vu 26 6 resemble resemble VBP cord-341342-kyavg4vu 26 7 the the DT cord-341342-kyavg4vu 26 8 NP NP NNP cord-341342-kyavg4vu 26 9 proteins protein NNS cord-341342-kyavg4vu 26 10 of of IN cord-341342-kyavg4vu 26 11 the the DT cord-341342-kyavg4vu 26 12 orthomyxoviruses orthomyxovirus NNS cord-341342-kyavg4vu 26 13 [ [ -LRB- cord-341342-kyavg4vu 26 14 1 1 CD cord-341342-kyavg4vu 26 15 , , , cord-341342-kyavg4vu 26 16 4 4 CD cord-341342-kyavg4vu 26 17 ] ] -RRB- cord-341342-kyavg4vu 26 18 . . . cord-341342-kyavg4vu 27 1 By by IN cord-341342-kyavg4vu 27 2 contrast contrast NN cord-341342-kyavg4vu 27 3 , , , cord-341342-kyavg4vu 27 4 the the DT cord-341342-kyavg4vu 27 5 N n CD cord-341342-kyavg4vu 27 6 -R -R . cord-341342-kyavg4vu 28 1 N N NNP cord-341342-kyavg4vu 28 2 A a DT cord-341342-kyavg4vu 28 3 complexes complex NNS cord-341342-kyavg4vu 28 4 of of IN cord-341342-kyavg4vu 28 5 the the DT cord-341342-kyavg4vu 28 6 rhabdoviruses rhabdovirus NNS cord-341342-kyavg4vu 28 7 and and CC cord-341342-kyavg4vu 28 8 the the DT cord-341342-kyavg4vu 28 9 paramyxoviruses paramyxovirus NNS cord-341342-kyavg4vu 28 10 , , , cord-341342-kyavg4vu 28 11 which which WDT cord-341342-kyavg4vu 28 12 also also RB cord-341342-kyavg4vu 28 13 form form VBP cord-341342-kyavg4vu 28 14 helically helically RB cord-341342-kyavg4vu 28 15 symmetric symmetric JJ cord-341342-kyavg4vu 28 16 nucteocapsids nucteocapsid NNS cord-341342-kyavg4vu 28 17 , , , cord-341342-kyavg4vu 28 18 are be VBP cord-341342-kyavg4vu 28 19 stable stable JJ cord-341342-kyavg4vu 28 20 in in IN cord-341342-kyavg4vu 28 21 high high JJ cord-341342-kyavg4vu 28 22 salt salt NN cord-341342-kyavg4vu 28 23 and and CC cord-341342-kyavg4vu 28 24 are be VBP cord-341342-kyavg4vu 28 25 largely largely RB cord-341342-kyavg4vu 28 26 resistant resistant JJ cord-341342-kyavg4vu 28 27 to to IN cord-341342-kyavg4vu 28 28 ribonuclease ribonuclease NN cord-341342-kyavg4vu 28 29 [ [ -LRB- cord-341342-kyavg4vu 28 30 1 1 CD cord-341342-kyavg4vu 28 31 , , , cord-341342-kyavg4vu 28 32 11 11 CD cord-341342-kyavg4vu 28 33 ] ] -RRB- cord-341342-kyavg4vu 28 34 . . . cord-341342-kyavg4vu 29 1 Few few JJ cord-341342-kyavg4vu 29 2 details detail NNS cord-341342-kyavg4vu 29 3 are be VBP cord-341342-kyavg4vu 29 4 known know VBN cord-341342-kyavg4vu 29 5 about about IN cord-341342-kyavg4vu 29 6 the the DT cord-341342-kyavg4vu 29 7 association association NN cord-341342-kyavg4vu 29 8 between between IN cord-341342-kyavg4vu 29 9 N N NNP cord-341342-kyavg4vu 29 10 protein protein NN cord-341342-kyavg4vu 29 11 and and CC cord-341342-kyavg4vu 29 12 RNA RNA NNP cord-341342-kyavg4vu 29 13 in in IN cord-341342-kyavg4vu 29 14 the the DT cord-341342-kyavg4vu 29 15 coronaviral coronaviral JJ cord-341342-kyavg4vu 29 16 nucleocapsid nucleocapsid NN cord-341342-kyavg4vu 29 17 . . . cord-341342-kyavg4vu 30 1 We -PRON- PRP cord-341342-kyavg4vu 30 2 have have VBP cord-341342-kyavg4vu 30 3 previously previously RB cord-341342-kyavg4vu 30 4 compared compare VBN cord-341342-kyavg4vu 30 5 the the DT cord-341342-kyavg4vu 30 6 amino amino NN cord-341342-kyavg4vu 30 7 acid acid NN cord-341342-kyavg4vu 30 8 sequences sequence NNS cord-341342-kyavg4vu 30 9 of of IN cord-341342-kyavg4vu 30 10 the the DT cord-341342-kyavg4vu 30 11 N n CD cord-341342-kyavg4vu 30 12 proteins protein NNS cord-341342-kyavg4vu 30 13 of of IN cord-341342-kyavg4vu 30 14 five five CD cord-341342-kyavg4vu 30 15 strains strain NNS cord-341342-kyavg4vu 30 16 of of IN cord-341342-kyavg4vu 30 17 MHV MHV NNP cord-341342-kyavg4vu 30 18 and and CC cord-341342-kyavg4vu 30 19 , , , cord-341342-kyavg4vu 30 20 on on IN cord-341342-kyavg4vu 30 21 the the DT cord-341342-kyavg4vu 30 22 basis basis NN cord-341342-kyavg4vu 30 23 of of IN cord-341342-kyavg4vu 30 24 this this DT cord-341342-kyavg4vu 30 25 analysis analysis NN cord-341342-kyavg4vu 30 26 , , , cord-341342-kyavg4vu 30 27 proposed propose VBD cord-341342-kyavg4vu 30 28 a a DT cord-341342-kyavg4vu 30 29 three three CD cord-341342-kyavg4vu 30 30 domain domain NN cord-341342-kyavg4vu 30 31 structural structural JJ cord-341342-kyavg4vu 30 32 model model NN cord-341342-kyavg4vu 30 33 for for IN cord-341342-kyavg4vu 30 34 the the DT cord-341342-kyavg4vu 30 35 M M NNP cord-341342-kyavg4vu 30 36 H H NNP cord-341342-kyavg4vu 30 37 V v NN cord-341342-kyavg4vu 30 38 N n NN cord-341342-kyavg4vu 30 39 protein protein NN cord-341342-kyavg4vu 30 40 [ [ -LRB- cord-341342-kyavg4vu 30 41 20 20 CD cord-341342-kyavg4vu 30 42 ] ] -RRB- cord-341342-kyavg4vu 30 43 . . . cord-341342-kyavg4vu 31 1 In in IN cord-341342-kyavg4vu 31 2 this this DT cord-341342-kyavg4vu 31 3 paper paper NN cord-341342-kyavg4vu 31 4 it -PRON- PRP cord-341342-kyavg4vu 31 5 is be VBZ cord-341342-kyavg4vu 31 6 shown show VBN cord-341342-kyavg4vu 31 7 that that IN cord-341342-kyavg4vu 31 8 the the DT cord-341342-kyavg4vu 31 9 central central NN cord-341342-kyavg4vu 31 10 of of IN cord-341342-kyavg4vu 31 11 these these DT cord-341342-kyavg4vu 31 12 three three CD cord-341342-kyavg4vu 31 13 domains domain NNS cord-341342-kyavg4vu 31 14 is be VBZ cord-341342-kyavg4vu 31 15 responsible responsible JJ cord-341342-kyavg4vu 31 16 for for IN cord-341342-kyavg4vu 31 17 the the DT cord-341342-kyavg4vu 31 18 RNA RNA NNP cord-341342-kyavg4vu 31 19 - - HYPH cord-341342-kyavg4vu 31 20 binding bind VBG cord-341342-kyavg4vu 31 21 ability ability NN cord-341342-kyavg4vu 31 22 of of IN cord-341342-kyavg4vu 31 23 the the DT cord-341342-kyavg4vu 31 24 MHV MHV NNP cord-341342-kyavg4vu 31 25 N N NNP cord-341342-kyavg4vu 31 26 molecule molecule NN cord-341342-kyavg4vu 31 27 . . . cord-341342-kyavg4vu 32 1 Transcription transcription NN cord-341342-kyavg4vu 32 2 vectors vector NNS cord-341342-kyavg4vu 32 3 linking link VBG cord-341342-kyavg4vu 32 4 the the DT cord-341342-kyavg4vu 32 5 N n CD cord-341342-kyavg4vu 32 6 gene gene NN cord-341342-kyavg4vu 32 7 of of IN cord-341342-kyavg4vu 32 8 MHV MHV NNP cord-341342-kyavg4vu 32 9 to to IN cord-341342-kyavg4vu 32 10 the the DT cord-341342-kyavg4vu 32 11 RNA RNA NNP cord-341342-kyavg4vu 32 12 polymerase polymerase NN cord-341342-kyavg4vu 32 13 promoter promoter NN cord-341342-kyavg4vu 32 14 of of IN cord-341342-kyavg4vu 32 15 either either DT cord-341342-kyavg4vu 32 16 bacteriophage bacteriophage NN cord-341342-kyavg4vu 32 17 T7 t7 NN cord-341342-kyavg4vu 32 18 or or CC cord-341342-kyavg4vu 32 19 bacteriophage bacteriophage NN cord-341342-kyavg4vu 32 20 SP6 sp6 NN cord-341342-kyavg4vu 32 21 were be VBD cord-341342-kyavg4vu 32 22 constructed construct VBN cord-341342-kyavg4vu 32 23 by by IN cord-341342-kyavg4vu 32 24 standard standard JJ cord-341342-kyavg4vu 32 25 recombinant recombinant JJ cord-341342-kyavg4vu 32 26 DNA dna NN cord-341342-kyavg4vu 32 27 techniques technique NNS cord-341342-kyavg4vu 32 28 [ [ -LRB- cord-341342-kyavg4vu 32 29 1 1 CD cord-341342-kyavg4vu 32 30 ] ] -RRB- cord-341342-kyavg4vu 32 31 [ [ -LRB- cord-341342-kyavg4vu 32 32 2 2 CD cord-341342-kyavg4vu 32 33 ] ] -RRB- cord-341342-kyavg4vu 32 34 [ [ -LRB- cord-341342-kyavg4vu 32 35 3 3 CD cord-341342-kyavg4vu 32 36 ] ] -RRB- cord-341342-kyavg4vu 32 37 [ [ -LRB- cord-341342-kyavg4vu 32 38 4 4 CD cord-341342-kyavg4vu 32 39 ] ] -RRB- cord-341342-kyavg4vu 32 40 [ [ -LRB- cord-341342-kyavg4vu 32 41 5 5 CD cord-341342-kyavg4vu 32 42 ] ] -RRB- cord-341342-kyavg4vu 32 43 [ [ -LRB- cord-341342-kyavg4vu 32 44 6 6 CD cord-341342-kyavg4vu 32 45 ] ] -RRB- cord-341342-kyavg4vu 32 46 [ [ -LRB- cord-341342-kyavg4vu 32 47 7 7 CD cord-341342-kyavg4vu 32 48 ] ] -RRB- cord-341342-kyavg4vu 32 49 [ [ -LRB- cord-341342-kyavg4vu 32 50 8 8 CD cord-341342-kyavg4vu 32 51 ] ] -RRB- cord-341342-kyavg4vu 33 1 [ [ -LRB- cord-341342-kyavg4vu 33 2 9 9 CD cord-341342-kyavg4vu 33 3 ] ] -RRB- cord-341342-kyavg4vu 33 4 [ [ -LRB- cord-341342-kyavg4vu 33 5 10 10 CD cord-341342-kyavg4vu 33 6 ] ] -RRB- cord-341342-kyavg4vu 33 7 [ [ -LRB- cord-341342-kyavg4vu 33 8 11 11 CD cord-341342-kyavg4vu 33 9 ] ] -RRB- cord-341342-kyavg4vu 33 10 [ [ -LRB- cord-341342-kyavg4vu 33 11 12 12 CD cord-341342-kyavg4vu 33 12 ] ] -RRB- cord-341342-kyavg4vu 33 13 [ [ -LRB- cord-341342-kyavg4vu 33 14 13 13 CD cord-341342-kyavg4vu 33 15 ] ] -RRB- cord-341342-kyavg4vu 33 16 [ [ -LRB- cord-341342-kyavg4vu 33 17 14 14 CD cord-341342-kyavg4vu 33 18 ] ] -RRB- cord-341342-kyavg4vu 33 19 . . . cord-341342-kyavg4vu 34 1 A a DT cord-341342-kyavg4vu 34 2 cDNA cdna NN cord-341342-kyavg4vu 34 3 clone clone NN cord-341342-kyavg4vu 34 4 , , , cord-341342-kyavg4vu 34 5 pAl5 pAl5 '' cord-341342-kyavg4vu 34 6 , , , cord-341342-kyavg4vu 34 7 containing contain VBG cord-341342-kyavg4vu 34 8 a a DT cord-341342-kyavg4vu 34 9 nearly nearly RB cord-341342-kyavg4vu 34 10 full full JJ cord-341342-kyavg4vu 34 11 - - HYPH cord-341342-kyavg4vu 34 12 length length NN cord-341342-kyavg4vu 34 13 copy copy NN cord-341342-kyavg4vu 34 14 of of IN cord-341342-kyavg4vu 34 15 the the DT cord-341342-kyavg4vu 34 16 N N NNP cord-341342-kyavg4vu 34 17 mRNA mrna NN cord-341342-kyavg4vu 34 18 of of IN cord-341342-kyavg4vu 34 19 MHV MHV NNP cord-341342-kyavg4vu 34 20 - - HYPH cord-341342-kyavg4vu 34 21 A59 A59 NNP cord-341342-kyavg4vu 34 22 [ [ -LRB- cord-341342-kyavg4vu 34 23 20 20 CD cord-341342-kyavg4vu 34 24 ] ] -RRB- cord-341342-kyavg4vu 35 1 ( ( -LRB- cord-341342-kyavg4vu 35 2 EMBL EMBL NNP cord-341342-kyavg4vu 35 3 / / SYM cord-341342-kyavg4vu 35 4 GenBank GenBank NNP cord-341342-kyavg4vu 35 5 accession accession NN cord-341342-kyavg4vu 36 1 no no UH cord-341342-kyavg4vu 36 2 . . . cord-341342-kyavg4vu 37 1 M35256 M35256 NNP cord-341342-kyavg4vu 37 2 ) ) -RRB- cord-341342-kyavg4vu 37 3 was be VBD cord-341342-kyavg4vu 37 4 restricted restrict VBN cord-341342-kyavg4vu 37 5 with with IN cord-341342-kyavg4vu 37 6 SnaBI SnaBI NNP cord-341342-kyavg4vu 37 7 and and CC cord-341342-kyavg4vu 37 8 SacI SacI NNP cord-341342-kyavg4vu 37 9 , , , cord-341342-kyavg4vu 37 10 made make VBD cord-341342-kyavg4vu 37 11 blunt blunt JJ cord-341342-kyavg4vu 37 12 - - HYPH cord-341342-kyavg4vu 37 13 ended ended JJ cord-341342-kyavg4vu 37 14 , , , cord-341342-kyavg4vu 37 15 and and CC cord-341342-kyavg4vu 37 16 inserted insert VBN cord-341342-kyavg4vu 37 17 in in IN cord-341342-kyavg4vu 37 18 either either DT cord-341342-kyavg4vu 37 19 orientation orientation NN cord-341342-kyavg4vu 37 20 into into IN cord-341342-kyavg4vu 37 21 the the DT cord-341342-kyavg4vu 37 22 vector vector NN cord-341342-kyavg4vu 37 23 pGEM3Zf pgem3zf NN cord-341342-kyavg4vu 37 24 ( ( -LRB- cord-341342-kyavg4vu 37 25 - - : cord-341342-kyavg4vu 37 26 ) ) -RRB- cord-341342-kyavg4vu 37 27 ( ( -LRB- cord-341342-kyavg4vu 37 28 Promega Promega NNP cord-341342-kyavg4vu 37 29 ) ) -RRB- cord-341342-kyavg4vu 37 30 , , , cord-341342-kyavg4vu 37 31 which which WDT cord-341342-kyavg4vu 37 32 had have VBD cord-341342-kyavg4vu 37 33 been be VBN cord-341342-kyavg4vu 37 34 opened open VBN cord-341342-kyavg4vu 37 35 and and CC cord-341342-kyavg4vu 37 36 blunt blunt RB cord-341342-kyavg4vu 37 37 - - HYPH cord-341342-kyavg4vu 37 38 ended ended JJ cord-341342-kyavg4vu 37 39 at at IN cord-341342-kyavg4vu 37 40 the the DT cord-341342-kyavg4vu 37 41 KpnI KpnI NNP cord-341342-kyavg4vu 37 42 and and CC cord-341342-kyavg4vu 37 43 SphI SphI NNP cord-341342-kyavg4vu 37 44 sites site NNS cord-341342-kyavg4vu 37 45 of of IN cord-341342-kyavg4vu 37 46 the the DT cord-341342-kyavg4vu 37 47 polylinker polylinker NN cord-341342-kyavg4vu 37 48 . . . cord-341342-kyavg4vu 38 1 The the DT cord-341342-kyavg4vu 38 2 resulting result VBG cord-341342-kyavg4vu 38 3 constructs construct NNS cord-341342-kyavg4vu 38 4 , , , cord-341342-kyavg4vu 38 5 pA50 pa50 NN cord-341342-kyavg4vu 38 6 and and CC cord-341342-kyavg4vu 38 7 pA48 pa48 NN cord-341342-kyavg4vu 38 8 , , , cord-341342-kyavg4vu 38 9 contained contain VBD cord-341342-kyavg4vu 38 10 the the DT cord-341342-kyavg4vu 38 11 T7 T7 NNP cord-341342-kyavg4vu 38 12 or or CC cord-341342-kyavg4vu 38 13 SP6 sp6 NN cord-341342-kyavg4vu 38 14 promoter promoter NN cord-341342-kyavg4vu 38 15 , , , cord-341342-kyavg4vu 38 16 respectively respectively RB cord-341342-kyavg4vu 38 17 , , , cord-341342-kyavg4vu 38 18 linked link VBN cord-341342-kyavg4vu 38 19 to to IN cord-341342-kyavg4vu 38 20 54 54 CD cord-341342-kyavg4vu 38 21 nt nt RB cord-341342-kyavg4vu 38 22 of of IN cord-341342-kyavg4vu 38 23 the the DT cord-341342-kyavg4vu 38 24 78 78 CD cord-341342-kyavg4vu 38 25 nt nt NN cord-341342-kyavg4vu 38 26 5 5 CD cord-341342-kyavg4vu 38 27 ' ' POS cord-341342-kyavg4vu 38 28 untranslated untranslated JJ cord-341342-kyavg4vu 38 29 region region NN cord-341342-kyavg4vu 38 30 ( ( -LRB- cord-341342-kyavg4vu 38 31 UTR UTR NNP cord-341342-kyavg4vu 38 32 ) ) -RRB- cord-341342-kyavg4vu 38 33 of of IN cord-341342-kyavg4vu 38 34 RNA7 RNA7 NNP cord-341342-kyavg4vu 38 35 of of IN cord-341342-kyavg4vu 38 36 MHV MHV NNP cord-341342-kyavg4vu 38 37 - - HYPH cord-341342-kyavg4vu 38 38 A59 A59 NNP cord-341342-kyavg4vu 38 39 I- I- NNP cord-341342-kyavg4vu 38 40 8 8 CD cord-341342-kyavg4vu 38 41 , , , cord-341342-kyavg4vu 38 42 19 19 CD cord-341342-kyavg4vu 38 43 ] ] -RRB- cord-341342-kyavg4vu 38 44 , , , cord-341342-kyavg4vu 38 45 followed follow VBN cord-341342-kyavg4vu 38 46 by by IN cord-341342-kyavg4vu 38 47 the the DT cord-341342-kyavg4vu 38 48 entire entire JJ cord-341342-kyavg4vu 38 49 N n CD cord-341342-kyavg4vu 38 50 protein protein NN cord-341342-kyavg4vu 38 51 coding code VBG cord-341342-kyavg4vu 38 52 region region NN cord-341342-kyavg4vu 38 53 and and CC cord-341342-kyavg4vu 38 54 225 225 CD cord-341342-kyavg4vu 38 55 nt nt RB cord-341342-kyavg4vu 38 56 of of IN cord-341342-kyavg4vu 38 57 the the DT cord-341342-kyavg4vu 38 58 301 301 CD cord-341342-kyavg4vu 38 59 nt nt NN cord-341342-kyavg4vu 38 60 3 3 CD cord-341342-kyavg4vu 38 61 ' ' '' cord-341342-kyavg4vu 38 62 UTR UTR NNP cord-341342-kyavg4vu 38 63 ( ( -LRB- cord-341342-kyavg4vu 38 64 Fig Fig NNP cord-341342-kyavg4vu 38 65 . . . cord-341342-kyavg4vu 38 66 1 1 CD cord-341342-kyavg4vu 38 67 ) ) -RRB- cord-341342-kyavg4vu 38 68 . . . cord-341342-kyavg4vu 39 1 HindIII HindIII NNP cord-341342-kyavg4vu 39 2 - - HYPH cord-341342-kyavg4vu 39 3 linearized linearize VBN cord-341342-kyavg4vu 39 4 pA50 pa50 NN cord-341342-kyavg4vu 39 5 encoded encode VBN cord-341342-kyavg4vu 39 6 run run NN cord-341342-kyavg4vu 39 7 - - HYPH cord-341342-kyavg4vu 39 8 off off RP cord-341342-kyavg4vu 39 9 transcripts transcript NNS cord-341342-kyavg4vu 39 10 that that WDT cord-341342-kyavg4vu 39 11 were be VBD cord-341342-kyavg4vu 39 12 bounded bound VBN cord-341342-kyavg4vu 39 13 by by IN cord-341342-kyavg4vu 39 14 polylinker polylinker NN cord-341342-kyavg4vu 39 15 sequences sequence NNS cord-341342-kyavg4vu 39 16 of of IN cord-341342-kyavg4vu 39 17 17 17 CD cord-341342-kyavg4vu 39 18 nt nt NN cord-341342-kyavg4vu 39 19 and and CC cord-341342-kyavg4vu 39 20 6 6 CD cord-341342-kyavg4vu 39 21 nt nt RB cord-341342-kyavg4vu 39 22 at at IN cord-341342-kyavg4vu 39 23 their -PRON- PRP$ cord-341342-kyavg4vu 39 24 5 5 CD cord-341342-kyavg4vu 39 25 ' ' '' cord-341342-kyavg4vu 39 26 and and CC cord-341342-kyavg4vu 39 27 3 3 CD cord-341342-kyavg4vu 39 28 ' ' POS cord-341342-kyavg4vu 39 29 ends end NNS cord-341342-kyavg4vu 39 30 ; ; : cord-341342-kyavg4vu 39 31 SacI SacI NNP cord-341342-kyavg4vu 39 32 - - HYPH cord-341342-kyavg4vu 39 33 linearized linearize VBN cord-341342-kyavg4vu 39 34 pA48 pa48 NN cord-341342-kyavg4vu 39 35 encoded encode VBN cord-341342-kyavg4vu 39 36 run run NN cord-341342-kyavg4vu 39 37 - - HYPH cord-341342-kyavg4vu 39 38 off off RP cord-341342-kyavg4vu 39 39 transcripts transcript NNS cord-341342-kyavg4vu 39 40 that that WDT cord-341342-kyavg4vu 39 41 were be VBD cord-341342-kyavg4vu 39 42 bounded bound VBN cord-341342-kyavg4vu 39 43 by by IN cord-341342-kyavg4vu 39 44 polylinker polylinker NN cord-341342-kyavg4vu 39 45 sequences sequence NNS cord-341342-kyavg4vu 39 46 of of IN cord-341342-kyavg4vu 39 47 15 15 CD cord-341342-kyavg4vu 39 48 nt nt NN cord-341342-kyavg4vu 39 49 and and CC cord-341342-kyavg4vu 39 50 2 2 CD cord-341342-kyavg4vu 39 51 nt nt RB cord-341342-kyavg4vu 39 52 at at IN cord-341342-kyavg4vu 39 53 their -PRON- PRP$ cord-341342-kyavg4vu 39 54 5 5 CD cord-341342-kyavg4vu 39 55 ' ' '' cord-341342-kyavg4vu 39 56 and and CC cord-341342-kyavg4vu 39 57 3 3 CD cord-341342-kyavg4vu 39 58 ' ' POS cord-341342-kyavg4vu 39 59 ends end NNS cord-341342-kyavg4vu 39 60 ( ( -LRB- cord-341342-kyavg4vu 39 61 Fig Fig NNP cord-341342-kyavg4vu 39 62 . . . cord-341342-kyavg4vu 39 63 1 1 CD cord-341342-kyavg4vu 39 64 ) ) -RRB- cord-341342-kyavg4vu 39 65 . . . cord-341342-kyavg4vu 40 1 Full full JJ cord-341342-kyavg4vu 40 2 - - HYPH cord-341342-kyavg4vu 40 3 length length NN cord-341342-kyavg4vu 41 1 N N NNP cord-341342-kyavg4vu 41 2 mRNAs mrnas XX cord-341342-kyavg4vu 41 3 transcribed transcribe VBD cord-341342-kyavg4vu 41 4 from from IN cord-341342-kyavg4vu 41 5 these these DT cord-341342-kyavg4vu 41 6 two two CD cord-341342-kyavg4vu 41 7 plasmids plasmid NNS cord-341342-kyavg4vu 41 8 had have VBD cord-341342-kyavg4vu 41 9 identical identical JJ cord-341342-kyavg4vu 41 10 translation translation NN cord-341342-kyavg4vu 41 11 efficiencies efficiency NNS cord-341342-kyavg4vu 41 12 in in IN cord-341342-kyavg4vu 41 13 vitro vitro FW cord-341342-kyavg4vu 41 14 . . . cord-341342-kyavg4vu 42 1 Truncated truncated JJ cord-341342-kyavg4vu 42 2 N N NNP cord-341342-kyavg4vu 42 3 mRNAs mRNAs NNPS cord-341342-kyavg4vu 42 4 were be VBD cord-341342-kyavg4vu 42 5 transcribed transcribe VBN cord-341342-kyavg4vu 42 6 from from IN cord-341342-kyavg4vu 42 7 pA50 pa50 NN cord-341342-kyavg4vu 42 8 that that WDT cord-341342-kyavg4vu 42 9 had have VBD cord-341342-kyavg4vu 42 10 been be VBN cord-341342-kyavg4vu 42 11 linearized linearize VBN cord-341342-kyavg4vu 42 12 with with IN cord-341342-kyavg4vu 42 13 BsmI BsmI NNP cord-341342-kyavg4vu 42 14 , , , cord-341342-kyavg4vu 42 15 ScaI ScaI NNP cord-341342-kyavg4vu 42 16 or or CC cord-341342-kyavg4vu 42 17 SpeI SpeI NNP cord-341342-kyavg4vu 42 18 and and CC cord-341342-kyavg4vu 42 19 from from IN cord-341342-kyavg4vu 42 20 pA48 pa48 NN cord-341342-kyavg4vu 43 1 that that DT cord-341342-kyavg4vu 43 2 had have VBD cord-341342-kyavg4vu 43 3 been be VBN cord-341342-kyavg4vu 43 4 linearized linearize VBN cord-341342-kyavg4vu 43 5 with with IN cord-341342-kyavg4vu 43 6 AccI AccI NNP cord-341342-kyavg4vu 43 7 or or CC cord-341342-kyavg4vu 43 8 EcoRI EcoRI NNP cord-341342-kyavg4vu 43 9 ( ( -LRB- cord-341342-kyavg4vu 43 10 Fig fig NN cord-341342-kyavg4vu 43 11 . . . cord-341342-kyavg4vu 43 12 1 1 CD cord-341342-kyavg4vu 43 13 ) ) -RRB- cord-341342-kyavg4vu 43 14 . . . cord-341342-kyavg4vu 44 1 An an DT cord-341342-kyavg4vu 44 2 internal internal JJ cord-341342-kyavg4vu 44 3 deletion deletion NN cord-341342-kyavg4vu 44 4 mutant mutant NN cord-341342-kyavg4vu 44 5 of of IN cord-341342-kyavg4vu 44 6 the the DT cord-341342-kyavg4vu 44 7 N N NNP cord-341342-kyavg4vu 44 8 gene gene NN cord-341342-kyavg4vu 44 9 was be VBD cord-341342-kyavg4vu 44 10 constructed construct VBN cord-341342-kyavg4vu 44 11 by by IN cord-341342-kyavg4vu 44 12 restriction restriction NN cord-341342-kyavg4vu 44 13 of of IN cord-341342-kyavg4vu 44 14 pA50 pa50 NN cord-341342-kyavg4vu 44 15 with with IN cord-341342-kyavg4vu 44 16 [ [ -LRB- cord-341342-kyavg4vu 44 17 20 20 CD cord-341342-kyavg4vu 44 18 ] ] -RRB- cord-341342-kyavg4vu 44 19 . . . cord-341342-kyavg4vu 45 1 The the DT cord-341342-kyavg4vu 45 2 N N NNP cord-341342-kyavg4vu 45 3 mRNA mRNA NNP cord-341342-kyavg4vu 45 4 initiation initiation NN cord-341342-kyavg4vu 45 5 codon codon NNP cord-341342-kyavg4vu 45 6 is be VBZ cord-341342-kyavg4vu 45 7 underlined underline VBN cord-341342-kyavg4vu 45 8 NheI NheI NNP cord-341342-kyavg4vu 45 9 and and CC cord-341342-kyavg4vu 45 10 SpeI SpeI NNP cord-341342-kyavg4vu 45 11 ( ( -LRB- cord-341342-kyavg4vu 45 12 Fig Fig NNP cord-341342-kyavg4vu 45 13 . . . cord-341342-kyavg4vu 45 14 1 1 CD cord-341342-kyavg4vu 45 15 ) ) -RRB- cord-341342-kyavg4vu 45 16 , , , cord-341342-kyavg4vu 45 17 followed follow VBN cord-341342-kyavg4vu 45 18 by by IN cord-341342-kyavg4vu 45 19 religation religation NN cord-341342-kyavg4vu 45 20 of of IN cord-341342-kyavg4vu 45 21 these these DT cord-341342-kyavg4vu 45 22 compatible compatible JJ cord-341342-kyavg4vu 45 23 sites site NNS cord-341342-kyavg4vu 45 24 in in IN cord-341342-kyavg4vu 45 25 the the DT cord-341342-kyavg4vu 45 26 vector vector NN cord-341342-kyavg4vu 45 27 fragment fragment NN cord-341342-kyavg4vu 45 28 . . . cord-341342-kyavg4vu 46 1 The the DT cord-341342-kyavg4vu 46 2 resulting result VBG cord-341342-kyavg4vu 46 3 plasmid plasmid NN cord-341342-kyavg4vu 46 4 , , , cord-341342-kyavg4vu 46 5 pA60 pA60 NNP cord-341342-kyavg4vu 46 6 , , , cord-341342-kyavg4vu 46 7 lacked lack VBD cord-341342-kyavg4vu 46 8 nt nt RB cord-341342-kyavg4vu 46 9 603 603 CD cord-341342-kyavg4vu 46 10 - - HYPH cord-341342-kyavg4vu 46 11 920 920 CD cord-341342-kyavg4vu 46 12 of of IN cord-341342-kyavg4vu 46 13 the the DT cord-341342-kyavg4vu 46 14 N n CD cord-341342-kyavg4vu 46 15 gene gene NN cord-341342-kyavg4vu 46 16 and and CC cord-341342-kyavg4vu 46 17 encoded encode VBD cord-341342-kyavg4vu 46 18 the the DT cord-341342-kyavg4vu 46 19 mutant mutant JJ cord-341342-kyavg4vu 46 20 protein protein NN cord-341342-kyavg4vu 46 21 N N NNP cord-341342-kyavg4vu 46 22 / / SYM cord-341342-kyavg4vu 46 23 Nhe Nhe NNP cord-341342-kyavg4vu 46 24 - - HYPH cord-341342-kyavg4vu 46 25 Spe Spe NNP cord-341342-kyavg4vu 46 26 . . . cord-341342-kyavg4vu 47 1 A a DT cord-341342-kyavg4vu 47 2 second second JJ cord-341342-kyavg4vu 47 3 internal internal JJ cord-341342-kyavg4vu 47 4 deletion deletion NN cord-341342-kyavg4vu 47 5 mutant mutant NN cord-341342-kyavg4vu 47 6 was be VBD cord-341342-kyavg4vu 47 7 generated generate VBN cord-341342-kyavg4vu 47 8 by by IN cord-341342-kyavg4vu 47 9 cleavage cleavage NN cord-341342-kyavg4vu 47 10 of of IN cord-341342-kyavg4vu 47 11 pA50 pa50 NN cord-341342-kyavg4vu 47 12 with with IN cord-341342-kyavg4vu 47 13 ApaI ApaI NNP cord-341342-kyavg4vu 47 14 and and CC cord-341342-kyavg4vu 47 15 NheI NheI NNP cord-341342-kyavg4vu 48 1 ( ( -LRB- cord-341342-kyavg4vu 48 2 Fig Fig NNP cord-341342-kyavg4vu 48 3 . . . cord-341342-kyavg4vu 48 4 1 1 CD cord-341342-kyavg4vu 48 5 KpnI KpnI NNP cord-341342-kyavg4vu 48 6 site site NN cord-341342-kyavg4vu 48 7 had have VBD cord-341342-kyavg4vu 48 8 been be VBN cord-341342-kyavg4vu 48 9 inserted insert VBN cord-341342-kyavg4vu 48 10 immediately immediately RB cord-341342-kyavg4vu 48 11 preceding precede VBG cord-341342-kyavg4vu 48 12 the the DT cord-341342-kyavg4vu 48 13 start start NN cord-341342-kyavg4vu 48 14 codon codon NN cord-341342-kyavg4vu 48 15 . . . cord-341342-kyavg4vu 49 1 Details detail NNS cord-341342-kyavg4vu 49 2 of of IN cord-341342-kyavg4vu 49 3 the the DT cord-341342-kyavg4vu 49 4 construction construction NN cord-341342-kyavg4vu 49 5 of of IN cord-341342-kyavg4vu 49 6 pA56 pA56 `` cord-341342-kyavg4vu 49 7 will will MD cord-341342-kyavg4vu 49 8 be be VB cord-341342-kyavg4vu 49 9 described describe VBN cord-341342-kyavg4vu 49 10 elsewhere elsewhere RB cord-341342-kyavg4vu 49 11 . . . cord-341342-kyavg4vu 50 1 The the DT cord-341342-kyavg4vu 50 2 KpnI(blunted)-HindlII KpnI(blunted)-HindlII NNP cord-341342-kyavg4vu 50 3 fragment fragment NN cord-341342-kyavg4vu 50 4 of of IN cord-341342-kyavg4vu 50 5 pA56 pA56 NNP cord-341342-kyavg4vu 50 6 was be VBD cord-341342-kyavg4vu 50 7 exchanged exchange VBN cord-341342-kyavg4vu 50 8 for for IN cord-341342-kyavg4vu 50 9 the the DT cord-341342-kyavg4vu 50 10 StuI StuI NNP cord-341342-kyavg4vu 50 11 - - HYPH cord-341342-kyavg4vu 50 12 HindlII HindlII NNP cord-341342-kyavg4vu 50 13 fragment fragment NN cord-341342-kyavg4vu 50 14 of of IN cord-341342-kyavg4vu 50 15 the the DT cord-341342-kyavg4vu 50 16 mutant mutant JJ cord-341342-kyavg4vu 50 17 VSV VSV NNP cord-341342-kyavg4vu 50 18 N N NNP cord-341342-kyavg4vu 50 19 SP6 SP6 NNP cord-341342-kyavg4vu 50 20 transcription transcription NN cord-341342-kyavg4vu 50 21 vector vector NN cord-341342-kyavg4vu 50 22 pN112 pn112 NN cord-341342-kyavg4vu 50 23 [ [ -LRB- cord-341342-kyavg4vu 50 24 16 16 CD cord-341342-kyavg4vu 50 25 ] ] -RRB- cord-341342-kyavg4vu 50 26 . . . cord-341342-kyavg4vu 51 1 This this DT cord-341342-kyavg4vu 51 2 yielded yield VBD cord-341342-kyavg4vu 51 3 a a DT cord-341342-kyavg4vu 51 4 plasmid plasmid NN cord-341342-kyavg4vu 51 5 , , , cord-341342-kyavg4vu 51 6 pA58 pa58 RB cord-341342-kyavg4vu 51 7 , , , cord-341342-kyavg4vu 51 8 containing contain VBG cord-341342-kyavg4vu 51 9 nt nt RB cord-341342-kyavg4vu 51 10 1 1 CD cord-341342-kyavg4vu 51 11 - - SYM cord-341342-kyavg4vu 51 12 69 69 CD cord-341342-kyavg4vu 51 13 and and CC cord-341342-kyavg4vu 51 14 370 370 CD cord-341342-kyavg4vu 51 15 - - SYM cord-341342-kyavg4vu 51 16 420 420 CD cord-341342-kyavg4vu 51 17 of of IN cord-341342-kyavg4vu 51 18 the the DT cord-341342-kyavg4vu 51 19 coding code VBG cord-341342-kyavg4vu 51 20 region region NN cord-341342-kyavg4vu 51 21 of of IN cord-341342-kyavg4vu 51 22 the the DT cord-341342-kyavg4vu 51 23 VSV VSV NNP cord-341342-kyavg4vu 51 24 N N NNP cord-341342-kyavg4vu 51 25 gene gene NN cord-341342-kyavg4vu 51 26 followed follow VBN cord-341342-kyavg4vu 51 27 by by IN cord-341342-kyavg4vu 51 28 the the DT cord-341342-kyavg4vu 51 29 entire entire JJ cord-341342-kyavg4vu 51 30 coding code VBG cord-341342-kyavg4vu 51 31 region region NN cord-341342-kyavg4vu 51 32 of of IN cord-341342-kyavg4vu 51 33 the the DT cord-341342-kyavg4vu 51 34 MHV MHV NNP cord-341342-kyavg4vu 51 35 N N NNP cord-341342-kyavg4vu 51 36 gene gene NN cord-341342-kyavg4vu 51 37 ; ; : cord-341342-kyavg4vu 51 38 the the DT cord-341342-kyavg4vu 51 39 encoded encode VBN cord-341342-kyavg4vu 51 40 protein protein NN cord-341342-kyavg4vu 51 41 was be VBD cord-341342-kyavg4vu 51 42 designated designate VBN cord-341342-kyavg4vu 51 43 N N NNP cord-341342-kyavg4vu 51 44 / / SYM cord-341342-kyavg4vu 51 45 fusl fusl NNS cord-341342-kyavg4vu 51 46 . . . cord-341342-kyavg4vu 52 1 A a DT cord-341342-kyavg4vu 52 2 deletion deletion NN cord-341342-kyavg4vu 52 3 mutant mutant NN cord-341342-kyavg4vu 52 4 of of IN cord-341342-kyavg4vu 52 5 pA58 pA58 NNP cord-341342-kyavg4vu 52 6 was be VBD cord-341342-kyavg4vu 52 7 created create VBN cord-341342-kyavg4vu 52 8 by by IN cord-341342-kyavg4vu 52 9 exchange exchange NN cord-341342-kyavg4vu 52 10 of of IN cord-341342-kyavg4vu 52 11 the the DT cord-341342-kyavg4vu 52 12 ApaI(blunted)-HindlII ApaI(blunted)-HindlII NNP cord-341342-kyavg4vu 52 13 fragment fragment NN cord-341342-kyavg4vu 52 14 of of IN cord-341342-kyavg4vu 52 15 pA50 pa50 NN cord-341342-kyavg4vu 52 16 for for IN cord-341342-kyavg4vu 52 17 the the DT cord-341342-kyavg4vu 52 18 StuI StuI NNP cord-341342-kyavg4vu 52 19 - - HYPH cord-341342-kyavg4vu 52 20 HindlII HindlII NNP cord-341342-kyavg4vu 52 21 fragment fragment NN cord-341342-kyavg4vu 52 22 of of IN cord-341342-kyavg4vu 52 23 pN112 pN112 NNP cord-341342-kyavg4vu 52 24 . . . cord-341342-kyavg4vu 53 1 The the DT cord-341342-kyavg4vu 53 2 resulting result VBG cord-341342-kyavg4vu 53 3 plasmid plasmid NN cord-341342-kyavg4vu 53 4 , , , cord-341342-kyavg4vu 53 5 pA63 pa63 NN cord-341342-kyavg4vu 53 6 , , , cord-341342-kyavg4vu 53 7 contained contain VBD cord-341342-kyavg4vu 53 8 nt nt RB cord-341342-kyavg4vu 53 9 1 1 CD cord-341342-kyavg4vu 53 10 - - SYM cord-341342-kyavg4vu 53 11 69 69 CD cord-341342-kyavg4vu 53 12 and and CC cord-341342-kyavg4vu 53 13 370 370 CD cord-341342-kyavg4vu 53 14 - - SYM cord-341342-kyavg4vu 53 15 420 420 CD cord-341342-kyavg4vu 53 16 of of IN cord-341342-kyavg4vu 53 17 the the DT cord-341342-kyavg4vu 53 18 VSV VSV NNP cord-341342-kyavg4vu 53 19 N N NNP cord-341342-kyavg4vu 53 20 gene gene NN cord-341342-kyavg4vu 53 21 followed follow VBN cord-341342-kyavg4vu 53 22 by by IN cord-341342-kyavg4vu 53 23 nt nt RB cord-341342-kyavg4vu 53 24 406 406 CD cord-341342-kyavg4vu 53 25 - - SYM cord-341342-kyavg4vu 53 26 1362 1362 CD cord-341342-kyavg4vu 53 27 of of IN cord-341342-kyavg4vu 53 28 the the DT cord-341342-kyavg4vu 53 29 MHV MHV NNP cord-341342-kyavg4vu 53 30 N N NNP cord-341342-kyavg4vu 53 31 gene gene NN cord-341342-kyavg4vu 53 32 ; ; : cord-341342-kyavg4vu 53 33 the the DT cord-341342-kyavg4vu 53 34 encoded encode VBN cord-341342-kyavg4vu 53 35 protein protein NN cord-341342-kyavg4vu 53 36 was be VBD cord-341342-kyavg4vu 53 37 designated designate VBN cord-341342-kyavg4vu 53 38 N N NNP cord-341342-kyavg4vu 53 39 / / SYM cord-341342-kyavg4vu 53 40 fus2 fus2 NNP cord-341342-kyavg4vu 53 41 . . . cord-341342-kyavg4vu 54 1 During during IN cord-341342-kyavg4vu 54 2 the the DT cord-341342-kyavg4vu 54 3 construction construction NN cord-341342-kyavg4vu 54 4 of of IN cord-341342-kyavg4vu 54 5 pA63 pa63 NN cord-341342-kyavg4vu 54 6 , , , cord-341342-kyavg4vu 54 7 there there EX cord-341342-kyavg4vu 54 8 serendipitously serendipitously RB cord-341342-kyavg4vu 54 9 arose arise VBD cord-341342-kyavg4vu 54 10 a a DT cord-341342-kyavg4vu 54 11 similar similar JJ cord-341342-kyavg4vu 54 12 plasmid plasmid NN cord-341342-kyavg4vu 54 13 , , , cord-341342-kyavg4vu 54 14 pA65 pa65 UH cord-341342-kyavg4vu 54 15 , , , cord-341342-kyavg4vu 54 16 containing contain VBG cord-341342-kyavg4vu 54 17 the the DT cord-341342-kyavg4vu 54 18 same same JJ cord-341342-kyavg4vu 54 19 extent extent NN cord-341342-kyavg4vu 54 20 of of IN cord-341342-kyavg4vu 54 21 MHV MHV NNP cord-341342-kyavg4vu 54 22 N n CD cord-341342-kyavg4vu 54 23 sequence sequence NN cord-341342-kyavg4vu 54 24 preceded precede VBD cord-341342-kyavg4vu 54 25 only only RB cord-341342-kyavg4vu 54 26 by by IN cord-341342-kyavg4vu 54 27 nt nt RB cord-341342-kyavg4vu 54 28 1 1 CD cord-341342-kyavg4vu 54 29 - - SYM cord-341342-kyavg4vu 54 30 24 24 CD cord-341342-kyavg4vu 54 31 of of IN cord-341342-kyavg4vu 54 32 the the DT cord-341342-kyavg4vu 54 33 VSV VSV NNP cord-341342-kyavg4vu 54 34 N N NNP cord-341342-kyavg4vu 54 35 gene gene NN cord-341342-kyavg4vu 54 36 ; ; : cord-341342-kyavg4vu 54 37 the the DT cord-341342-kyavg4vu 54 38 protein protein NN cord-341342-kyavg4vu 54 39 encoded encode VBN cord-341342-kyavg4vu 54 40 by by IN cord-341342-kyavg4vu 54 41 this this DT cord-341342-kyavg4vu 54 42 construct construct NN cord-341342-kyavg4vu 54 43 was be VBD cord-341342-kyavg4vu 54 44 designated designate VBN cord-341342-kyavg4vu 54 45 N N NNP cord-341342-kyavg4vu 54 46 / / SYM cord-341342-kyavg4vu 54 47 fus4 fus4 NNP cord-341342-kyavg4vu 54 48 . . . cord-341342-kyavg4vu 55 1 All all DT cord-341342-kyavg4vu 55 2 plasmid plasmid NN cord-341342-kyavg4vu 55 3 constructs construct NNS cord-341342-kyavg4vu 55 4 were be VBD cord-341342-kyavg4vu 55 5 checked check VBN cord-341342-kyavg4vu 55 6 by by IN cord-341342-kyavg4vu 55 7 restriction restriction NN cord-341342-kyavg4vu 55 8 analysis analysis NN cord-341342-kyavg4vu 55 9 , , , cord-341342-kyavg4vu 55 10 and and CC cord-341342-kyavg4vu 55 11 all all DT cord-341342-kyavg4vu 55 12 newly newly RB cord-341342-kyavg4vu 55 13 formed form VBN cord-341342-kyavg4vu 55 14 junctions junction NNS cord-341342-kyavg4vu 55 15 were be VBD cord-341342-kyavg4vu 55 16 directly directly RB cord-341342-kyavg4vu 55 17 verified verify VBN cord-341342-kyavg4vu 55 18 by by IN cord-341342-kyavg4vu 55 19 dideoxy dideoxy NN cord-341342-kyavg4vu 55 20 sequencing sequencing NN cord-341342-kyavg4vu 55 21 [ [ -LRB- cord-341342-kyavg4vu 55 22 24 24 CD cord-341342-kyavg4vu 55 23 ] ] -RRB- cord-341342-kyavg4vu 55 24 . . . cord-341342-kyavg4vu 56 1 DNA DNA NNP cord-341342-kyavg4vu 56 2 plasmids plasmid NNS cord-341342-kyavg4vu 56 3 to to TO cord-341342-kyavg4vu 56 4 be be VB cord-341342-kyavg4vu 56 5 used use VBN cord-341342-kyavg4vu 56 6 as as IN cord-341342-kyavg4vu 56 7 transcription transcription NN cord-341342-kyavg4vu 56 8 templates template NNS cord-341342-kyavg4vu 56 9 were be VBD cord-341342-kyavg4vu 56 10 purified purify VBN cord-341342-kyavg4vu 56 11 by by IN cord-341342-kyavg4vu 56 12 two two CD cord-341342-kyavg4vu 56 13 cycles cycle NNS cord-341342-kyavg4vu 56 14 of of IN cord-341342-kyavg4vu 56 15 equilibrium equilibrium NN cord-341342-kyavg4vu 56 16 centrifugation centrifugation NN cord-341342-kyavg4vu 56 17 in in IN cord-341342-kyavg4vu 56 18 CsCI CsCI NNP cord-341342-kyavg4vu 56 19 gradients gradient NNS cord-341342-kyavg4vu 56 20 in in IN cord-341342-kyavg4vu 56 21 the the DT cord-341342-kyavg4vu 56 22 presence presence NN cord-341342-kyavg4vu 56 23 of of IN cord-341342-kyavg4vu 56 24 propidium propidium NN cord-341342-kyavg4vu 56 25 iodide iodide NN cord-341342-kyavg4vu 56 26 [ [ -LRB- cord-341342-kyavg4vu 56 27 14 14 CD cord-341342-kyavg4vu 56 28 ] ] -RRB- cord-341342-kyavg4vu 56 29 . . . cord-341342-kyavg4vu 57 1 Capped capped JJ cord-341342-kyavg4vu 57 2 , , , cord-341342-kyavg4vu 57 3 run run VB cord-341342-kyavg4vu 57 4 - - HYPH cord-341342-kyavg4vu 57 5 off off RP cord-341342-kyavg4vu 57 6 SP6 sp6 NN cord-341342-kyavg4vu 57 7 and and CC cord-341342-kyavg4vu 57 8 T7 t7 NN cord-341342-kyavg4vu 58 1 mRNAs mrnas LS cord-341342-kyavg4vu 58 2 [ [ -LRB- cord-341342-kyavg4vu 58 3 18 18 CD cord-341342-kyavg4vu 58 4 ] ] -RRB- cord-341342-kyavg4vu 58 5 were be VBD cord-341342-kyavg4vu 58 6 typically typically RB cord-341342-kyavg4vu 58 7 synthesized synthesize VBN cord-341342-kyavg4vu 58 8 in in IN cord-341342-kyavg4vu 58 9 100 100 CD cord-341342-kyavg4vu 58 10 gl gl NNP cord-341342-kyavg4vu 58 11 reactions reaction NNS cord-341342-kyavg4vu 58 12 containing contain VBG cord-341342-kyavg4vu 58 13 40mM 40mm CD cord-341342-kyavg4vu 58 14 Tris Tris NNP cord-341342-kyavg4vu 58 15 hydrochloride hydrochloride NN cord-341342-kyavg4vu 58 16 ( ( -LRB- cord-341342-kyavg4vu 58 17 pHT.9 pht.9 RB cord-341342-kyavg4vu 58 18 ) ) -RRB- cord-341342-kyavg4vu 58 19 , , , cord-341342-kyavg4vu 58 20 6mM 6mm CD cord-341342-kyavg4vu 58 21 MgC12 MgC12 NNP cord-341342-kyavg4vu 58 22 , , , cord-341342-kyavg4vu 58 23 2mM 2mm CD cord-341342-kyavg4vu 58 24 spermidine spermidine NN cord-341342-kyavg4vu 58 25 , , , cord-341342-kyavg4vu 58 26 10mM 10mm CD cord-341342-kyavg4vu 58 27 NaC1 NaC1 NNPS cord-341342-kyavg4vu 58 28 , , , cord-341342-kyavg4vu 58 29 10 10 CD cord-341342-kyavg4vu 58 30 mM mm CD cord-341342-kyavg4vu 58 31 dithiothreitoi dithiothreitoi NN cord-341342-kyavg4vu 58 32 , , , cord-341342-kyavg4vu 58 33 I00 I00 NNP cord-341342-kyavg4vu 58 34 U U NNP cord-341342-kyavg4vu 58 35 RNasin RNasin NNP cord-341342-kyavg4vu 58 36 ( ( -LRB- cord-341342-kyavg4vu 58 37 Promega Promega NNP cord-341342-kyavg4vu 58 38 ) ) -RRB- cord-341342-kyavg4vu 58 39 , , , cord-341342-kyavg4vu 58 40 500 500 CD cord-341342-kyavg4vu 58 41 gM gm NN cord-341342-kyavg4vu 59 1 each each DT cord-341342-kyavg4vu 59 2 ATP ATP NNP cord-341342-kyavg4vu 59 3 and and CC cord-341342-kyavg4vu 59 4 CTP CTP NNP cord-341342-kyavg4vu 59 5 , , , cord-341342-kyavg4vu 59 6 100 100 CD cord-341342-kyavg4vu 59 7 gM gm NN cord-341342-kyavg4vu 60 1 each each DT cord-341342-kyavg4vu 60 2 GTP GTP NNP cord-341342-kyavg4vu 60 3 and and CC cord-341342-kyavg4vu 60 4 UTP UTP NNP cord-341342-kyavg4vu 60 5 , , , cord-341342-kyavg4vu 60 6 500 500 CD cord-341342-kyavg4vu 60 7 IIM IIM NNP cord-341342-kyavg4vu 61 1 mVGpppG mVGpppG `` cord-341342-kyavg4vu 61 2 ( ( -LRB- cord-341342-kyavg4vu 61 3 New New NNP cord-341342-kyavg4vu 61 4 England England NNP cord-341342-kyavg4vu 61 5 Biolabs Biolabs NNP cord-341342-kyavg4vu 61 6 ) ) -RRB- cord-341342-kyavg4vu 61 7 , , . cord-341342-kyavg4vu 62 1 10 10 CD cord-341342-kyavg4vu 62 2 gCi gCi VBN cord-341342-kyavg4vu 62 3 [ [ -LRB- cord-341342-kyavg4vu 62 4 5,6 5,6 CD cord-341342-kyavg4vu 62 5 - - HYPH cord-341342-kyavg4vu 62 6 3H]UTP 3h]utp CD cord-341342-kyavg4vu 62 7 ( ( -LRB- cord-341342-kyavg4vu 62 8 45 45 CD cord-341342-kyavg4vu 62 9 Ci/ Ci/ NNP cord-341342-kyavg4vu 62 10 mmol mmol NN cord-341342-kyavg4vu 62 11 ; ; : cord-341342-kyavg4vu 62 12 Amersham Amersham NNP cord-341342-kyavg4vu 62 13 ) ) -RRB- cord-341342-kyavg4vu 62 14 , , , cord-341342-kyavg4vu 62 15 2 2 CD cord-341342-kyavg4vu 62 16 gg gg NNP cord-341342-kyavg4vu 62 17 linearized linearize VBN cord-341342-kyavg4vu 62 18 DNA dna NN cord-341342-kyavg4vu 62 19 template template NN cord-341342-kyavg4vu 62 20 , , , cord-341342-kyavg4vu 62 21 and and CC cord-341342-kyavg4vu 62 22 either either RB cord-341342-kyavg4vu 62 23 40 40 CD cord-341342-kyavg4vu 62 24 U U NNP cord-341342-kyavg4vu 62 25 SP6 SP6 NNP cord-341342-kyavg4vu 62 26 RNA RNA NNP cord-341342-kyavg4vu 62 27 polymerase polymerase NN cord-341342-kyavg4vu 62 28 or or CC cord-341342-kyavg4vu 62 29 80 80 CD cord-341342-kyavg4vu 62 30 U U NNP cord-341342-kyavg4vu 62 31 T7 T7 NNP cord-341342-kyavg4vu 62 32 RNA RNA NNP cord-341342-kyavg4vu 62 33 polymerase polymerase NN cord-341342-kyavg4vu 62 34 ( ( -LRB- cord-341342-kyavg4vu 62 35 New New NNP cord-341342-kyavg4vu 62 36 England England NNP cord-341342-kyavg4vu 62 37 Biolabs Biolabs NNP cord-341342-kyavg4vu 62 38 ) ) -RRB- cord-341342-kyavg4vu 62 39 . . . cord-341342-kyavg4vu 63 1 Reactions reaction NNS cord-341342-kyavg4vu 63 2 were be VBD cord-341342-kyavg4vu 63 3 carried carry VBN cord-341342-kyavg4vu 63 4 out out RP cord-341342-kyavg4vu 63 5 for for IN cord-341342-kyavg4vu 63 6 60 60 CD cord-341342-kyavg4vu 63 7 rain rain NN cord-341342-kyavg4vu 63 8 at at IN cord-341342-kyavg4vu 63 9 either either CC cord-341342-kyavg4vu 63 10 40 40 CD cord-341342-kyavg4vu 63 11 ° ° NN cord-341342-kyavg4vu 63 12 C c NN cord-341342-kyavg4vu 63 13 ( ( -LRB- cord-341342-kyavg4vu 63 14 SP6 SP6 NNP cord-341342-kyavg4vu 63 15 ) ) -RRB- cord-341342-kyavg4vu 63 16 or or CC cord-341342-kyavg4vu 63 17 37 37 CD cord-341342-kyavg4vu 63 18 ° ° NN cord-341342-kyavg4vu 63 19 C c NN cord-341342-kyavg4vu 63 20 ( ( -LRB- cord-341342-kyavg4vu 63 21 T7 T7 NNP cord-341342-kyavg4vu 63 22 ) ) -RRB- cord-341342-kyavg4vu 63 23 . . . cord-341342-kyavg4vu 64 1 Products product NNS cord-341342-kyavg4vu 64 2 were be VBD cord-341342-kyavg4vu 64 3 extracted extract VBN cord-341342-kyavg4vu 64 4 twice twice RB cord-341342-kyavg4vu 64 5 with with IN cord-341342-kyavg4vu 64 6 phenol phenol NN cord-341342-kyavg4vu 64 7 - - HYPH cord-341342-kyavg4vu 64 8 chloroform chloroform NN cord-341342-kyavg4vu 64 9 , , , cord-341342-kyavg4vu 64 10 twice twice RB cord-341342-kyavg4vu 64 11 with with IN cord-341342-kyavg4vu 64 12 chloroform chloroform NN cord-341342-kyavg4vu 64 13 and and CC cord-341342-kyavg4vu 64 14 were be VBD cord-341342-kyavg4vu 64 15 twice twice RB cord-341342-kyavg4vu 64 16 precipitated precipitate VBN cord-341342-kyavg4vu 64 17 with with IN cord-341342-kyavg4vu 64 18 ethanol ethanol NN cord-341342-kyavg4vu 64 19 . . . cord-341342-kyavg4vu 65 1 The the DT cord-341342-kyavg4vu 65 2 incorporation incorporation NN cord-341342-kyavg4vu 65 3 of of IN cord-341342-kyavg4vu 65 4 labeled label VBN cord-341342-kyavg4vu 65 5 UTP UTP NNP cord-341342-kyavg4vu 65 6 was be VBD cord-341342-kyavg4vu 65 7 determined determine VBN cord-341342-kyavg4vu 65 8 by by IN cord-341342-kyavg4vu 65 9 binding bind VBG cord-341342-kyavg4vu 65 10 of of IN cord-341342-kyavg4vu 65 11 product product NN cord-341342-kyavg4vu 65 12 RNA RNA NNP cord-341342-kyavg4vu 65 13 to to IN cord-341342-kyavg4vu 65 14 DEAE DEAE NNP cord-341342-kyavg4vu 65 15 filter filter NN cord-341342-kyavg4vu 65 16 paper paper NN cord-341342-kyavg4vu 66 1 ( ( -LRB- cord-341342-kyavg4vu 66 2 Whatman Whatman NNP cord-341342-kyavg4vu 66 3 DE DE NNP cord-341342-kyavg4vu 66 4 81 81 CD cord-341342-kyavg4vu 66 5 ) ) -RRB- cord-341342-kyavg4vu 67 1 ; ; : cord-341342-kyavg4vu 67 2 the the DT cord-341342-kyavg4vu 67 3 size size NN cord-341342-kyavg4vu 67 4 and and CC cord-341342-kyavg4vu 67 5 homogeneity homogeneity NN cord-341342-kyavg4vu 67 6 of of IN cord-341342-kyavg4vu 67 7 synthesized synthesize VBN cord-341342-kyavg4vu 67 8 RNA RNA NNP cord-341342-kyavg4vu 67 9 were be VBD cord-341342-kyavg4vu 67 10 verified verify VBN cord-341342-kyavg4vu 67 11 by by IN cord-341342-kyavg4vu 67 12 electrophoresis electrophoresis NN cord-341342-kyavg4vu 67 13 in in IN cord-341342-kyavg4vu 67 14 5 5 CD cord-341342-kyavg4vu 67 15 % % NN cord-341342-kyavg4vu 67 16 polyacrylamide polyacrylamide NN cord-341342-kyavg4vu 67 17 gels gel NNS cord-341342-kyavg4vu 67 18 containing contain VBG cord-341342-kyavg4vu 67 19 8 8 CD cord-341342-kyavg4vu 67 20 M M NNP cord-341342-kyavg4vu 67 21 urea urea NN cord-341342-kyavg4vu 67 22 , , , cord-341342-kyavg4vu 67 23 followed follow VBN cord-341342-kyavg4vu 67 24 by by IN cord-341342-kyavg4vu 67 25 fluorography fluorography NNP cord-341342-kyavg4vu 67 26 . . . cord-341342-kyavg4vu 68 1 For for IN cord-341342-kyavg4vu 68 2 the the DT cord-341342-kyavg4vu 68 3 synthesis synthesis NN cord-341342-kyavg4vu 68 4 of of IN cord-341342-kyavg4vu 68 5 high high JJ cord-341342-kyavg4vu 68 6 specific specific JJ cord-341342-kyavg4vu 68 7 activity activity NN cord-341342-kyavg4vu 68 8 , , , cord-341342-kyavg4vu 68 9 32p 32p CD cord-341342-kyavg4vu 68 10 - - HYPH cord-341342-kyavg4vu 68 11 labeled label VBN cord-341342-kyavg4vu 68 12 N N NNP cord-341342-kyavg4vu 68 13 mRNA mrna NN cord-341342-kyavg4vu 68 14 , , , cord-341342-kyavg4vu 68 15 all all DT cord-341342-kyavg4vu 68 16 reaction reaction NN cord-341342-kyavg4vu 68 17 conditions condition NNS cord-341342-kyavg4vu 68 18 were be VBD cord-341342-kyavg4vu 68 19 identical identical JJ cord-341342-kyavg4vu 68 20 , , , cord-341342-kyavg4vu 68 21 except except IN cord-341342-kyavg4vu 68 22 that that IN cord-341342-kyavg4vu 68 23 the the DT cord-341342-kyavg4vu 68 24 labeling labeling NN cord-341342-kyavg4vu 68 25 nucleotide nucleotide NN cord-341342-kyavg4vu 68 26 was be VBD cord-341342-kyavg4vu 68 27 100 100 CD cord-341342-kyavg4vu 68 28 ~tCi ~tCi NFP cord-341342-kyavg4vu 69 1 [ [ -LRB- cord-341342-kyavg4vu 69 2 a-32p]UTP a-32p]utp JJ cord-341342-kyavg4vu 69 3 ( ( -LRB- cord-341342-kyavg4vu 69 4 3000 3000 CD cord-341342-kyavg4vu 69 5 Ci ci NN cord-341342-kyavg4vu 69 6 / / SYM cord-341342-kyavg4vu 69 7 mmol mmol NN cord-341342-kyavg4vu 69 8 ; ; : cord-341342-kyavg4vu 69 9 Amersham Amersham NNP cord-341342-kyavg4vu 69 10 ) ) -RRB- cord-341342-kyavg4vu 69 11 . . . cord-341342-kyavg4vu 70 1 Translation translation NN cord-341342-kyavg4vu 70 2 of of IN cord-341342-kyavg4vu 70 3 synthetic synthetic NN cord-341342-kyavg4vu 70 4 mRNAs mRNAs NNPS cord-341342-kyavg4vu 70 5 was be VBD cord-341342-kyavg4vu 70 6 carried carry VBN cord-341342-kyavg4vu 70 7 out out RP cord-341342-kyavg4vu 70 8 in in IN cord-341342-kyavg4vu 70 9 a a DT cord-341342-kyavg4vu 70 10 micrococcal micrococcal JJ cord-341342-kyavg4vu 70 11 nuclease nuclease NN cord-341342-kyavg4vu 70 12 - - HYPH cord-341342-kyavg4vu 70 13 treated treat VBN cord-341342-kyavg4vu 70 14 rabbit rabbit NN cord-341342-kyavg4vu 70 15 reticulocyte reticulocyte NN cord-341342-kyavg4vu 70 16 lysate lysate NN cord-341342-kyavg4vu 70 17 ( ( -LRB- cord-341342-kyavg4vu 70 18 Amersham Amersham NNP cord-341342-kyavg4vu 70 19 ) ) -RRB- cord-341342-kyavg4vu 70 20 . . . cord-341342-kyavg4vu 71 1 In in IN cord-341342-kyavg4vu 71 2 the the DT cord-341342-kyavg4vu 71 3 standard standard JJ cord-341342-kyavg4vu 71 4 reaction reaction NN cord-341342-kyavg4vu 71 5 , , , cord-341342-kyavg4vu 71 6 0.3 0.3 CD cord-341342-kyavg4vu 71 7 to to TO cord-341342-kyavg4vu 71 8 0.4 0.4 CD cord-341342-kyavg4vu 71 9 ~tg ~tg NNS cord-341342-kyavg4vu 71 10 of of IN cord-341342-kyavg4vu 71 11 mRNA mrna NN cord-341342-kyavg4vu 71 12 in in IN cord-341342-kyavg4vu 71 13 3 3 CD cord-341342-kyavg4vu 71 14 to to IN cord-341342-kyavg4vu 71 15 4~tl 4~tl CD cord-341342-kyavg4vu 71 16 of of IN cord-341342-kyavg4vu 71 17 H20 H20 NNP cord-341342-kyavg4vu 71 18 was be VBD cord-341342-kyavg4vu 71 19 used use VBN cord-341342-kyavg4vu 71 20 to to TO cord-341342-kyavg4vu 71 21 program program VB cord-341342-kyavg4vu 71 22 12gl 12gl NN cord-341342-kyavg4vu 71 23 of of IN cord-341342-kyavg4vu 71 24 reticulocyte reticulocyte NNP cord-341342-kyavg4vu 71 25 lysate lysate NN cord-341342-kyavg4vu 71 26 containing contain VBG cord-341342-kyavg4vu 71 27 14U 14u CD cord-341342-kyavg4vu 71 28 of of IN cord-341342-kyavg4vu 71 29 RNasin RNasin NNP cord-341342-kyavg4vu 71 30 and and CC cord-341342-kyavg4vu 71 31 8 8 CD cord-341342-kyavg4vu 71 32 to to TO cord-341342-kyavg4vu 71 33 10 10 CD cord-341342-kyavg4vu 71 34 gCi gCi NNS cord-341342-kyavg4vu 71 35 of of IN cord-341342-kyavg4vu 71 36 [ [ -LRB- cord-341342-kyavg4vu 71 37 35S]methionine 35s]methionine NN cord-341342-kyavg4vu 71 38 ( ( -LRB- cord-341342-kyavg4vu 71 39 > > NFP cord-341342-kyavg4vu 71 40 1100 1100 CD cord-341342-kyavg4vu 71 41 Ci ci NN cord-341342-kyavg4vu 71 42 / / SYM cord-341342-kyavg4vu 71 43 mmol mmol NN cord-341342-kyavg4vu 71 44 ; ; : cord-341342-kyavg4vu 71 45 New New NNP cord-341342-kyavg4vu 71 46 England England NNP cord-341342-kyavg4vu 71 47 Nuclear Nuclear NNP cord-341342-kyavg4vu 71 48 ) ) -RRB- cord-341342-kyavg4vu 71 49 . . . cord-341342-kyavg4vu 72 1 Incubations incubation NNS cord-341342-kyavg4vu 72 2 were be VBD cord-341342-kyavg4vu 72 3 carried carry VBN cord-341342-kyavg4vu 72 4 out out RP cord-341342-kyavg4vu 72 5 for for IN cord-341342-kyavg4vu 72 6 90 90 CD cord-341342-kyavg4vu 72 7 rain rain NN cord-341342-kyavg4vu 72 8 at at IN cord-341342-kyavg4vu 72 9 30 30 CD cord-341342-kyavg4vu 72 10 ° ° NN cord-341342-kyavg4vu 72 11 C c NN cord-341342-kyavg4vu 72 12 . . . cord-341342-kyavg4vu 73 1 In in IN cord-341342-kyavg4vu 73 2 many many JJ cord-341342-kyavg4vu 73 3 experiments experiment NNS cord-341342-kyavg4vu 73 4 , , , cord-341342-kyavg4vu 73 5 where where WRB cord-341342-kyavg4vu 73 6 noted note VBN cord-341342-kyavg4vu 73 7 , , , cord-341342-kyavg4vu 73 8 1 1 CD cord-341342-kyavg4vu 73 9 gl gl NN cord-341342-kyavg4vu 73 10 of of IN cord-341342-kyavg4vu 73 11 either either DT cord-341342-kyavg4vu 73 12 H20 H20 NNP cord-341342-kyavg4vu 73 13 or or CC cord-341342-kyavg4vu 73 14 RNase RNase NNP cord-341342-kyavg4vu 73 15 A A NNP cord-341342-kyavg4vu 73 16 was be VBD cord-341342-kyavg4vu 73 17 then then RB cord-341342-kyavg4vu 73 18 added add VBN cord-341342-kyavg4vu 73 19 , , , cord-341342-kyavg4vu 73 20 and and CC cord-341342-kyavg4vu 73 21 samples sample NNS cord-341342-kyavg4vu 73 22 were be VBD cord-341342-kyavg4vu 73 23 incubated incubate VBN cord-341342-kyavg4vu 73 24 an an DT cord-341342-kyavg4vu 73 25 additional additional JJ cord-341342-kyavg4vu 73 26 30 30 CD cord-341342-kyavg4vu 73 27 rain rain NN cord-341342-kyavg4vu 73 28 at at IN cord-341342-kyavg4vu 73 29 30 30 CD cord-341342-kyavg4vu 73 30 ° ° NN cord-341342-kyavg4vu 73 31 C c NN cord-341342-kyavg4vu 73 32 . . . cord-341342-kyavg4vu 74 1 Bovine bovine JJ cord-341342-kyavg4vu 74 2 pancreatic pancreatic NN cord-341342-kyavg4vu 75 1 RNase RNase NNP cord-341342-kyavg4vu 75 2 A A NNP cord-341342-kyavg4vu 75 3 ( ( -LRB- cord-341342-kyavg4vu 75 4 ca ca NN cord-341342-kyavg4vu 75 5 . . . cord-341342-kyavg4vu 76 1 100 100 CD cord-341342-kyavg4vu 76 2 U U NNP cord-341342-kyavg4vu 76 3 / / SYM cord-341342-kyavg4vu 76 4 mg mg NN cord-341342-kyavg4vu 76 5 ; ; : cord-341342-kyavg4vu 76 6 Sigma Sigma NNP cord-341342-kyavg4vu 76 7 type type NN cord-341342-kyavg4vu 76 8 XII XII NNP cord-341342-kyavg4vu 76 9 - - HYPH cord-341342-kyavg4vu 76 10 A A NNP cord-341342-kyavg4vu 76 11 ) ) -RRB- cord-341342-kyavg4vu 76 12 was be VBD cord-341342-kyavg4vu 76 13 rendered render VBN cord-341342-kyavg4vu 76 14 DNase DNase NNP cord-341342-kyavg4vu 76 15 - - HYPH cord-341342-kyavg4vu 76 16 free free JJ cord-341342-kyavg4vu 76 17 by by IN cord-341342-kyavg4vu 76 18 heat heat NN cord-341342-kyavg4vu 76 19 treatment treatment NN cord-341342-kyavg4vu 76 20 [ [ -LRB- cord-341342-kyavg4vu 76 21 14 14 CD cord-341342-kyavg4vu 76 22 ] ] -RRB- cord-341342-kyavg4vu 76 23 . . . cord-341342-kyavg4vu 77 1 For for IN cord-341342-kyavg4vu 77 2 translation translation NN cord-341342-kyavg4vu 77 3 of of IN cord-341342-kyavg4vu 77 4 authentic authentic JJ cord-341342-kyavg4vu 77 5 MHV MHV NNP cord-341342-kyavg4vu 77 6 mRNA mRNA NNP cord-341342-kyavg4vu 77 7 , , , cord-341342-kyavg4vu 77 8 total total JJ cord-341342-kyavg4vu 77 9 RNA RNA NNP cord-341342-kyavg4vu 77 10 was be VBD cord-341342-kyavg4vu 77 11 purified purify VBN cord-341342-kyavg4vu 77 12 [ [ -LRB- cord-341342-kyavg4vu 77 13 5 5 CD cord-341342-kyavg4vu 77 14 ] ] -RRB- cord-341342-kyavg4vu 77 15 from from IN cord-341342-kyavg4vu 77 16 MHV MHV NNP cord-341342-kyavg4vu 77 17 - - HYPH cord-341342-kyavg4vu 77 18 A59-infected A59-infected NNP cord-341342-kyavg4vu 77 19 ( ( -LRB- cord-341342-kyavg4vu 77 20 as as RB cord-341342-kyavg4vu 77 21 well well RB cord-341342-kyavg4vu 77 22 as as IN cord-341342-kyavg4vu 77 23 mock mock NN cord-341342-kyavg4vu 77 24 - - HYPH cord-341342-kyavg4vu 77 25 infected infected JJ cord-341342-kyavg4vu 77 26 ) ) -RRB- cord-341342-kyavg4vu 77 27 mouse mouse NN cord-341342-kyavg4vu 77 28 17 17 CD cord-341342-kyavg4vu 77 29 clone clone NN cord-341342-kyavg4vu 77 30 1 1 CD cord-341342-kyavg4vu 77 31 cells cell NNS cord-341342-kyavg4vu 77 32 at at IN cord-341342-kyavg4vu 77 33 20 20 CD cord-341342-kyavg4vu 77 34 h h NN cord-341342-kyavg4vu 77 35 postinfection postinfection NN cord-341342-kyavg4vu 77 36 , , , cord-341342-kyavg4vu 77 37 and and CC cord-341342-kyavg4vu 77 38 6 6 CD cord-341342-kyavg4vu 77 39 gg gg NNP cord-341342-kyavg4vu 77 40 was be VBD cord-341342-kyavg4vu 77 41 translated translate VBN cord-341342-kyavg4vu 77 42 as as IN cord-341342-kyavg4vu 77 43 above above RB cord-341342-kyavg4vu 77 44 . . . cord-341342-kyavg4vu 78 1 In in IN cord-341342-kyavg4vu 78 2 vitro vitro FW cord-341342-kyavg4vu 78 3 - - HYPH cord-341342-kyavg4vu 78 4 synthesized synthesize VBN cord-341342-kyavg4vu 78 5 proteins protein NNS cord-341342-kyavg4vu 78 6 were be VBD cord-341342-kyavg4vu 78 7 analyzed analyze VBN cord-341342-kyavg4vu 78 8 both both CC cord-341342-kyavg4vu 78 9 by by IN cord-341342-kyavg4vu 78 10 sodium sodium NN cord-341342-kyavg4vu 78 11 dodecyl dodecyl NNP cord-341342-kyavg4vu 78 12 sulfate sulfate NN cord-341342-kyavg4vu 78 13 polyacrylamide polyacrylamide NN cord-341342-kyavg4vu 78 14 gel gel NN cord-341342-kyavg4vu 78 15 electrophoresis electrophoresis NN cord-341342-kyavg4vu 78 16 ( ( -LRB- cord-341342-kyavg4vu 78 17 SDS SDS NNP cord-341342-kyavg4vu 78 18 - - HYPH cord-341342-kyavg4vu 78 19 PAGE PAGE NNP cord-341342-kyavg4vu 78 20 ) ) -RRB- cord-341342-kyavg4vu 79 1 [ [ -LRB- cord-341342-kyavg4vu 79 2 7 7 CD cord-341342-kyavg4vu 79 3 ] ] -RRB- cord-341342-kyavg4vu 79 4 and and CC cord-341342-kyavg4vu 79 5 by by IN cord-341342-kyavg4vu 79 6 nondenaturing nondenature VBG cord-341342-kyavg4vu 79 7 PAGE page NN cord-341342-kyavg4vu 79 8 employing employ VBG cord-341342-kyavg4vu 79 9 the the DT cord-341342-kyavg4vu 79 10 standard standard JJ cord-341342-kyavg4vu 79 11 Laemmli Laemmli NNP cord-341342-kyavg4vu 79 12 discontinuous discontinuous JJ cord-341342-kyavg4vu 79 13 system system NN cord-341342-kyavg4vu 79 14 in in IN cord-341342-kyavg4vu 79 15 which which WDT cord-341342-kyavg4vu 79 16 SDS SDS NNP cord-341342-kyavg4vu 79 17 was be VBD cord-341342-kyavg4vu 79 18 omitted omit VBN cord-341342-kyavg4vu 79 19 from from IN cord-341342-kyavg4vu 79 20 all all DT cord-341342-kyavg4vu 79 21 gels gel NNS cord-341342-kyavg4vu 79 22 and and CC cord-341342-kyavg4vu 79 23 buffers buffer NNS cord-341342-kyavg4vu 79 24 , , , cord-341342-kyavg4vu 79 25 and and CC cord-341342-kyavg4vu 79 26 samples sample NNS cord-341342-kyavg4vu 79 27 were be VBD cord-341342-kyavg4vu 79 28 not not RB cord-341342-kyavg4vu 79 29 heated heat VBN cord-341342-kyavg4vu 79 30 prior prior RB cord-341342-kyavg4vu 79 31 to to IN cord-341342-kyavg4vu 79 32 electrophoresis electrophoresis NN cord-341342-kyavg4vu 79 33 [ [ -LRB- cord-341342-kyavg4vu 79 34 15 15 CD cord-341342-kyavg4vu 79 35 ] ] -RRB- cord-341342-kyavg4vu 79 36 . . . cord-341342-kyavg4vu 80 1 Reticulocyte Reticulocyte NNP cord-341342-kyavg4vu 80 2 lysate lysate NN cord-341342-kyavg4vu 80 3 samples sample NNS cord-341342-kyavg4vu 80 4 ( ( -LRB- cord-341342-kyavg4vu 80 5 4111 4111 CD cord-341342-kyavg4vu 80 6 ) ) -RRB- cord-341342-kyavg4vu 80 7 were be VBD cord-341342-kyavg4vu 80 8 run run VBN cord-341342-kyavg4vu 80 9 on on IN cord-341342-kyavg4vu 80 10 SDS SDS NNP cord-341342-kyavg4vu 80 11 - - HYPH cord-341342-kyavg4vu 80 12 PAGE page NN cord-341342-kyavg4vu 80 13 gels gel NNS cord-341342-kyavg4vu 80 14 ( ( -LRB- cord-341342-kyavg4vu 80 15 10 10 CD cord-341342-kyavg4vu 80 16 % % NN cord-341342-kyavg4vu 80 17 acrylamide-0.27 acrylamide-0.27 NN cord-341342-kyavg4vu 80 18 % % NN cord-341342-kyavg4vu 80 19 methylene methylene NN cord-341342-kyavg4vu 80 20 bisacrylamide bisacrylamide NNP cord-341342-kyavg4vu 80 21 ) ) -RRB- cord-341342-kyavg4vu 80 22 and and CC cord-341342-kyavg4vu 80 23 nondenaturing nondenature VBG cord-341342-kyavg4vu 80 24 PAGE page NN cord-341342-kyavg4vu 80 25 gels gel NNS cord-341342-kyavg4vu 80 26 ( ( -LRB- cord-341342-kyavg4vu 80 27 7.5 7.5 CD cord-341342-kyavg4vu 80 28 % % NN cord-341342-kyavg4vu 80 29 acrylamide-0.2 acrylamide-0.2 VBP cord-341342-kyavg4vu 80 30 % % NN cord-341342-kyavg4vu 80 31 methylene methylene NN cord-341342-kyavg4vu 80 32 bisacrylamide bisacrylamide NN cord-341342-kyavg4vu 80 33 ) ) -RRB- cord-341342-kyavg4vu 80 34 that that WDT cord-341342-kyavg4vu 80 35 were be VBD cord-341342-kyavg4vu 80 36 19 19 CD cord-341342-kyavg4vu 80 37 cm cm NNS cord-341342-kyavg4vu 80 38 long long JJ cord-341342-kyavg4vu 80 39 by by IN cord-341342-kyavg4vu 80 40 1.5 1.5 CD cord-341342-kyavg4vu 80 41 mm mm NN cord-341342-kyavg4vu 80 42 thick thick JJ cord-341342-kyavg4vu 80 43 . . . cord-341342-kyavg4vu 81 1 Nondenaturing Nondenaturing NNP cord-341342-kyavg4vu 81 2 PAGE PAGE NNP cord-341342-kyavg4vu 81 3 was be VBD cord-341342-kyavg4vu 81 4 performed perform VBN cord-341342-kyavg4vu 81 5 at at IN cord-341342-kyavg4vu 81 6 80 80 CD cord-341342-kyavg4vu 81 7 V v NN cord-341342-kyavg4vu 81 8 at at IN cord-341342-kyavg4vu 81 9 ambient ambient JJ cord-341342-kyavg4vu 81 10 temperature temperature NN cord-341342-kyavg4vu 81 11 ( ( -LRB- cord-341342-kyavg4vu 81 12 ca ca NN cord-341342-kyavg4vu 81 13 . . . cord-341342-kyavg4vu 82 1 18 18 CD cord-341342-kyavg4vu 82 2 h h NN cord-341342-kyavg4vu 82 3 ) ) -RRB- cord-341342-kyavg4vu 82 4 ; ; : cord-341342-kyavg4vu 82 5 under under IN cord-341342-kyavg4vu 82 6 these these DT cord-341342-kyavg4vu 82 7 conditions condition NNS cord-341342-kyavg4vu 82 8 there there EX cord-341342-kyavg4vu 82 9 was be VBD cord-341342-kyavg4vu 82 10 no no DT cord-341342-kyavg4vu 82 11 detectable detectable JJ cord-341342-kyavg4vu 82 12 heating heating NN cord-341342-kyavg4vu 82 13 of of IN cord-341342-kyavg4vu 82 14 the the DT cord-341342-kyavg4vu 82 15 gel gel NN cord-341342-kyavg4vu 82 16 for for IN cord-341342-kyavg4vu 82 17 the the DT cord-341342-kyavg4vu 82 18 duration duration NN cord-341342-kyavg4vu 82 19 of of IN cord-341342-kyavg4vu 82 20 the the DT cord-341342-kyavg4vu 82 21 run run NN cord-341342-kyavg4vu 82 22 . . . cord-341342-kyavg4vu 83 1 Gels gel NNS cord-341342-kyavg4vu 83 2 were be VBD cord-341342-kyavg4vu 83 3 fixed fix VBN cord-341342-kyavg4vu 83 4 in in IN cord-341342-kyavg4vu 83 5 50 50 CD cord-341342-kyavg4vu 83 6 % % NN cord-341342-kyavg4vu 83 7 methanol-10 methanol-10 CD cord-341342-kyavg4vu 83 8 % % NN cord-341342-kyavg4vu 83 9 acetic acetic JJ cord-341342-kyavg4vu 83 10 acid acid NN cord-341342-kyavg4vu 83 11 and and CC cord-341342-kyavg4vu 83 12 were be VBD cord-341342-kyavg4vu 83 13 impregnated impregnate VBN cord-341342-kyavg4vu 83 14 with with IN cord-341342-kyavg4vu 83 15 1 1 CD cord-341342-kyavg4vu 83 16 M M NNP cord-341342-kyavg4vu 83 17 sodium sodium NN cord-341342-kyavg4vu 83 18 salicylate salicylate NN cord-341342-kyavg4vu 83 19 prior prior RB cord-341342-kyavg4vu 83 20 to to IN cord-341342-kyavg4vu 83 21 drying drying NN cord-341342-kyavg4vu 83 22 and and CC cord-341342-kyavg4vu 83 23 fluorography fluorography NN cord-341342-kyavg4vu 83 24 at at IN cord-341342-kyavg4vu 83 25 -70 -70 NN cord-341342-kyavg4vu 83 26 ° ° NN cord-341342-kyavg4vu 83 27 C c NN cord-341342-kyavg4vu 83 28 . . . cord-341342-kyavg4vu 84 1 [ [ -LRB- cord-341342-kyavg4vu 84 2 3SS]Methionine 3ss]methionine NN cord-341342-kyavg4vu 84 3 - - HYPH cord-341342-kyavg4vu 84 4 labeled label VBN cord-341342-kyavg4vu 84 5 bands band NNS cord-341342-kyavg4vu 84 6 were be VBD cord-341342-kyavg4vu 84 7 quantitated quantitate VBN cord-341342-kyavg4vu 84 8 either either CC cord-341342-kyavg4vu 84 9 by by IN cord-341342-kyavg4vu 84 10 ( ( -LRB- cord-341342-kyavg4vu 84 11 / / SYM cord-341342-kyavg4vu 84 12 ) ) -RRB- cord-341342-kyavg4vu 84 13 excision excision NN cord-341342-kyavg4vu 84 14 and and CC cord-341342-kyavg4vu 84 15 solubilization solubilization NN cord-341342-kyavg4vu 84 16 in in IN cord-341342-kyavg4vu 84 17 2 2 CD cord-341342-kyavg4vu 84 18 ml ml NNS cord-341342-kyavg4vu 84 19 of of IN cord-341342-kyavg4vu 84 20 30 30 CD cord-341342-kyavg4vu 84 21 % % NN cord-341342-kyavg4vu 84 22 H202 h202 NN cord-341342-kyavg4vu 84 23 for for IN cord-341342-kyavg4vu 84 24 48 48 CD cord-341342-kyavg4vu 84 25 h h NN cord-341342-kyavg4vu 84 26 at at IN cord-341342-kyavg4vu 84 27 50 50 CD cord-341342-kyavg4vu 84 28 oC oC NNP cord-341342-kyavg4vu 85 1 prior prior RB cord-341342-kyavg4vu 85 2 to to IN cord-341342-kyavg4vu 85 3 counting count VBG cord-341342-kyavg4vu 85 4 in in IN cord-341342-kyavg4vu 85 5 8 8 CD cord-341342-kyavg4vu 85 6 ml ml NNS cord-341342-kyavg4vu 85 7 of of IN cord-341342-kyavg4vu 85 8 Aquasol Aquasol NNP cord-341342-kyavg4vu 85 9 ( ( -LRB- cord-341342-kyavg4vu 85 10 New New NNP cord-341342-kyavg4vu 85 11 England England NNP cord-341342-kyavg4vu 85 12 Nuclear Nuclear NNP cord-341342-kyavg4vu 85 13 ) ) -RRB- cord-341342-kyavg4vu 85 14 or or CC cord-341342-kyavg4vu 85 15 ( ( -LRB- cord-341342-kyavg4vu 85 16 ii ii NN cord-341342-kyavg4vu 85 17 ) ) -RRB- cord-341342-kyavg4vu 85 18 direct direct JJ cord-341342-kyavg4vu 85 19 counting counting NN cord-341342-kyavg4vu 85 20 with with IN cord-341342-kyavg4vu 85 21 a a DT cord-341342-kyavg4vu 85 22 Betascope Betascope NNP cord-341342-kyavg4vu 85 23 603 603 CD cord-341342-kyavg4vu 85 24 Blot blot NN cord-341342-kyavg4vu 85 25 Analyzer Analyzer NNP cord-341342-kyavg4vu 85 26 ( ( -LRB- cord-341342-kyavg4vu 85 27 Betagen Betagen NNP cord-341342-kyavg4vu 85 28 ) ) -RRB- cord-341342-kyavg4vu 85 29 . . . cord-341342-kyavg4vu 86 1 In in IN cord-341342-kyavg4vu 86 2 order order NN cord-341342-kyavg4vu 86 3 to to TO cord-341342-kyavg4vu 86 4 develop develop VB cord-341342-kyavg4vu 86 5 in in IN cord-341342-kyavg4vu 86 6 vitro vitro FW cord-341342-kyavg4vu 86 7 assays assay NNS cord-341342-kyavg4vu 86 8 for for IN cord-341342-kyavg4vu 86 9 MHV MHV NNP cord-341342-kyavg4vu 86 10 N N NNP cord-341342-kyavg4vu 86 11 protein protein NN cord-341342-kyavg4vu 86 12 function function NN cord-341342-kyavg4vu 86 13 , , , cord-341342-kyavg4vu 86 14 the the DT cord-341342-kyavg4vu 86 15 N N NNP cord-341342-kyavg4vu 86 16 gene gene NN cord-341342-kyavg4vu 86 17 was be VBD cord-341342-kyavg4vu 86 18 inserted insert VBN cord-341342-kyavg4vu 86 19 into into IN cord-341342-kyavg4vu 86 20 bacteriophage bacteriophage NN cord-341342-kyavg4vu 86 21 T7 t7 NN cord-341342-kyavg4vu 86 22 and and CC cord-341342-kyavg4vu 86 23 SP6 sp6 NN cord-341342-kyavg4vu 86 24 transcription transcription NN cord-341342-kyavg4vu 86 25 vectors vector NNS cord-341342-kyavg4vu 86 26 . . . cord-341342-kyavg4vu 87 1 Synthetic synthetic JJ cord-341342-kyavg4vu 87 2 mRNA mrna NN cord-341342-kyavg4vu 87 3 containing contain VBG cord-341342-kyavg4vu 87 4 the the DT cord-341342-kyavg4vu 87 5 entire entire JJ cord-341342-kyavg4vu 87 6 coding code VBG cord-341342-kyavg4vu 87 7 region region NN cord-341342-kyavg4vu 87 8 of of IN cord-341342-kyavg4vu 87 9 the the DT cord-341342-kyavg4vu 87 10 authentic authentic JJ cord-341342-kyavg4vu 87 11 N N NNP cord-341342-kyavg4vu 87 12 mRNA mRNA NNP cord-341342-kyavg4vu 87 13 , , , cord-341342-kyavg4vu 87 14 as as RB cord-341342-kyavg4vu 87 15 well well RB cord-341342-kyavg4vu 87 16 as as IN cord-341342-kyavg4vu 87 17 most most JJS cord-341342-kyavg4vu 87 18 of of IN cord-341342-kyavg4vu 87 19 the the DT cord-341342-kyavg4vu 87 20 5 5 CD cord-341342-kyavg4vu 87 21 ' ' '' cord-341342-kyavg4vu 87 22 and and CC cord-341342-kyavg4vu 87 23 3 3 CD cord-341342-kyavg4vu 87 24 ' ' POS cord-341342-kyavg4vu 87 25 UTRs utr NNS cord-341342-kyavg4vu 87 26 , , , cord-341342-kyavg4vu 87 27 was be VBD cord-341342-kyavg4vu 87 28 used use VBN cord-341342-kyavg4vu 87 29 to to TO cord-341342-kyavg4vu 87 30 program program VB cord-341342-kyavg4vu 87 31 a a DT cord-341342-kyavg4vu 87 32 protein protein NN cord-341342-kyavg4vu 87 33 synthesizing synthesize VBG cord-341342-kyavg4vu 87 34 system system NN cord-341342-kyavg4vu 87 35 from from IN cord-341342-kyavg4vu 87 36 rabbit rabbit NN cord-341342-kyavg4vu 87 37 reticulocyte reticulocyte NNP cord-341342-kyavg4vu 87 38 lysate lysate NN cord-341342-kyavg4vu 87 39 . . . cord-341342-kyavg4vu 88 1 The the DT cord-341342-kyavg4vu 88 2 translation translation NN cord-341342-kyavg4vu 88 3 product product NN cord-341342-kyavg4vu 88 4 from from IN cord-341342-kyavg4vu 88 5 this this DT cord-341342-kyavg4vu 88 6 message message NN cord-341342-kyavg4vu 88 7 , , , cord-341342-kyavg4vu 88 8 labeled label VBN cord-341342-kyavg4vu 88 9 with with IN cord-341342-kyavg4vu 88 10 [ [ -LRB- cord-341342-kyavg4vu 88 11 35S]methionine 35s]methionine CD cord-341342-kyavg4vu 88 12 , , , cord-341342-kyavg4vu 88 13 was be VBD cord-341342-kyavg4vu 88 14 analyzed analyze VBN cord-341342-kyavg4vu 88 15 by by IN cord-341342-kyavg4vu 88 16 SDS SDS NNP cord-341342-kyavg4vu 88 17 - - HYPH cord-341342-kyavg4vu 88 18 PAGE PAGE NNP cord-341342-kyavg4vu 88 19 and and CC cord-341342-kyavg4vu 88 20 had have VBD cord-341342-kyavg4vu 88 21 a a DT cord-341342-kyavg4vu 88 22 mobility mobility NN cord-341342-kyavg4vu 88 23 indistinguishable indistinguishable JJ cord-341342-kyavg4vu 88 24 from from IN cord-341342-kyavg4vu 88 25 that that DT cord-341342-kyavg4vu 88 26 of of IN cord-341342-kyavg4vu 88 27 N n CD cord-341342-kyavg4vu 88 28 protein protein NN cord-341342-kyavg4vu 88 29 translated translate VBN cord-341342-kyavg4vu 88 30 in in IN cord-341342-kyavg4vu 88 31 vitro vitro FW cord-341342-kyavg4vu 88 32 from from IN cord-341342-kyavg4vu 88 33 total total JJ cord-341342-kyavg4vu 88 34 RNA RNA NNP cord-341342-kyavg4vu 88 35 purified purify VBN cord-341342-kyavg4vu 88 36 from from IN cord-341342-kyavg4vu 88 37 MHV MHV NNP cord-341342-kyavg4vu 88 38 - - HYPH cord-341342-kyavg4vu 88 39 A59-infected a59-infecte VBN cord-341342-kyavg4vu 88 40 cells cell NNS cord-341342-kyavg4vu 88 41 ( ( -LRB- cord-341342-kyavg4vu 88 42 Fig fig NN cord-341342-kyavg4vu 88 43 . . . cord-341342-kyavg4vu 88 44 2 2 CD cord-341342-kyavg4vu 88 45 A A NNP cord-341342-kyavg4vu 88 46 , , , cord-341342-kyavg4vu 88 47 lanes lane NNS cord-341342-kyavg4vu 88 48 b b NN cord-341342-kyavg4vu 88 49 and and CC cord-341342-kyavg4vu 88 50 c c NN cord-341342-kyavg4vu 88 51 ) ) -RRB- cord-341342-kyavg4vu 88 52 . . . cord-341342-kyavg4vu 89 1 In in IN cord-341342-kyavg4vu 89 2 addition addition NN cord-341342-kyavg4vu 89 3 , , , cord-341342-kyavg4vu 89 4 N n CD cord-341342-kyavg4vu 89 5 protein protein NN cord-341342-kyavg4vu 89 6 translated translate VBD cord-341342-kyavg4vu 89 7 from from IN cord-341342-kyavg4vu 89 8 synthetic synthetic JJ cord-341342-kyavg4vu 89 9 mRNA mRNA NNP cord-341342-kyavg4vu 89 10 was be VBD cord-341342-kyavg4vu 89 11 immunoprecipitable immunoprecipitable JJ cord-341342-kyavg4vu 89 12 by by IN cord-341342-kyavg4vu 89 13 either either DT cord-341342-kyavg4vu 89 14 anti anti NNP cord-341342-kyavg4vu 89 15 - - JJ cord-341342-kyavg4vu 89 16 N n JJ cord-341342-kyavg4vu 89 17 or or CC cord-341342-kyavg4vu 89 18 anti anti JJ cord-341342-kyavg4vu 89 19 - - JJ cord-341342-kyavg4vu 89 20 MHV mhv JJ cord-341342-kyavg4vu 89 21 polyclonat polyclonat NN cord-341342-kyavg4vu 89 22 antibodies antibody NNS cord-341342-kyavg4vu 89 23 ( ( -LRB- cord-341342-kyavg4vu 89 24 Peng Peng NNP cord-341342-kyavg4vu 89 25 and and CC cord-341342-kyavg4vu 89 26 Masters Masters NNPS cord-341342-kyavg4vu 89 27 , , , cord-341342-kyavg4vu 89 28 unpubl unpubl NNS cord-341342-kyavg4vu 89 29 . . . cord-341342-kyavg4vu 90 1 data datum NNS cord-341342-kyavg4vu 90 2 ) ) -RRB- cord-341342-kyavg4vu 90 3 . . . cord-341342-kyavg4vu 91 1 In in IN cord-341342-kyavg4vu 91 2 vitro vitro FW cord-341342-kyavg4vu 91 3 - - HYPH cord-341342-kyavg4vu 91 4 translated translate VBN cord-341342-kyavg4vu 91 5 N n CD cord-341342-kyavg4vu 91 6 protein protein NN cord-341342-kyavg4vu 91 7 had have VBD cord-341342-kyavg4vu 91 8 a a DT cord-341342-kyavg4vu 91 9 slightly slightly RB cord-341342-kyavg4vu 91 10 higher high JJR cord-341342-kyavg4vu 91 11 apparent apparent JJ cord-341342-kyavg4vu 91 12 molecular molecular JJ cord-341342-kyavg4vu 91 13 weight weight NN cord-341342-kyavg4vu 91 14 than than IN cord-341342-kyavg4vu 91 15 N n CD cord-341342-kyavg4vu 91 16 protein protein NN cord-341342-kyavg4vu 91 17 from from IN cord-341342-kyavg4vu 91 18 [ [ -LRB- cord-341342-kyavg4vu 91 19 35S]methionine 35s]methionine CD cord-341342-kyavg4vu 91 20 - - HYPH cord-341342-kyavg4vu 91 21 labeled label VBN cord-341342-kyavg4vu 91 22 virus virus NN cord-341342-kyavg4vu 91 23 of of IN cord-341342-kyavg4vu 91 24 MHV MHV NNP cord-341342-kyavg4vu 91 25 ( ( -LRB- cord-341342-kyavg4vu 91 26 Fig fig NN cord-341342-kyavg4vu 91 27 . . . cord-341342-kyavg4vu 91 28 2 2 CD cord-341342-kyavg4vu 91 29 A A NNP cord-341342-kyavg4vu 91 30 , , , cord-341342-kyavg4vu 91 31 lane lane NN cord-341342-kyavg4vu 91 32 a a DT cord-341342-kyavg4vu 91 33 ) ) -RRB- cord-341342-kyavg4vu 91 34 . . . cord-341342-kyavg4vu 92 1 This this DT cord-341342-kyavg4vu 92 2 was be VBD cord-341342-kyavg4vu 92 3 possibly possibly RB cord-341342-kyavg4vu 92 4 due due JJ cord-341342-kyavg4vu 92 5 to to IN cord-341342-kyavg4vu 92 6 differential differential JJ cord-341342-kyavg4vu 92 7 extents extent NNS cord-341342-kyavg4vu 92 8 of of IN cord-341342-kyavg4vu 92 9 N n CD cord-341342-kyavg4vu 92 10 protein protein NN cord-341342-kyavg4vu 92 11 phosphorylation phosphorylation NN cord-341342-kyavg4vu 92 12 [ [ -LRB- cord-341342-kyavg4vu 92 13 30 30 CD cord-341342-kyavg4vu 92 14 ] ] -RRB- cord-341342-kyavg4vu 92 15 in in IN cord-341342-kyavg4vu 92 16 vitro vitro FW cord-341342-kyavg4vu 92 17 and and CC cord-341342-kyavg4vu 92 18 in in IN cord-341342-kyavg4vu 92 19 vivo vivo NN cord-341342-kyavg4vu 92 20 , , , cord-341342-kyavg4vu 92 21 or or CC cord-341342-kyavg4vu 92 22 it -PRON- PRP cord-341342-kyavg4vu 92 23 may may MD cord-341342-kyavg4vu 92 24 indicate indicate VB cord-341342-kyavg4vu 92 25 that that IN cord-341342-kyavg4vu 92 26 the the DT cord-341342-kyavg4vu 92 27 mature mature JJ cord-341342-kyavg4vu 92 28 N N NNP cord-341342-kyavg4vu 92 29 protein protein NN cord-341342-kyavg4vu 92 30 found find VBN cord-341342-kyavg4vu 92 31 in in IN cord-341342-kyavg4vu 92 32 MHV MHV NNP cord-341342-kyavg4vu 92 33 virions virion NNS cord-341342-kyavg4vu 92 34 had have VBD cord-341342-kyavg4vu 92 35 undergone undergo VBN cord-341342-kyavg4vu 92 36 amino amino NN cord-341342-kyavg4vu 92 37 - - HYPH cord-341342-kyavg4vu 92 38 terminal terminal NN cord-341342-kyavg4vu 92 39 modification modification NN cord-341342-kyavg4vu 92 40 , , , cord-341342-kyavg4vu 92 41 as as IN cord-341342-kyavg4vu 92 42 has have VBZ cord-341342-kyavg4vu 92 43 been be VBN cord-341342-kyavg4vu 92 44 observed observe VBN cord-341342-kyavg4vu 92 45 for for IN cord-341342-kyavg4vu 92 46 the the DT cord-341342-kyavg4vu 92 47 N n CD cord-341342-kyavg4vu 92 48 protein protein NN cord-341342-kyavg4vu 92 49 of of IN cord-341342-kyavg4vu 92 50 the the DT cord-341342-kyavg4vu 92 51 coronavirus coronavirus NN cord-341342-kyavg4vu 92 52 avian avian JJ cord-341342-kyavg4vu 92 53 infectious infectious JJ cord-341342-kyavg4vu 92 54 bronchitis bronchitis NN cord-341342-kyavg4vu 92 55 virus virus NN cord-341342-kyavg4vu 92 56 [ [ -LRB- cord-341342-kyavg4vu 92 57 2 2 CD cord-341342-kyavg4vu 92 58 ] ] -RRB- cord-341342-kyavg4vu 92 59 . . . cord-341342-kyavg4vu 93 1 This this DT cord-341342-kyavg4vu 93 2 apparent apparent JJ cord-341342-kyavg4vu 93 3 difference difference NN cord-341342-kyavg4vu 93 4 does do VBZ cord-341342-kyavg4vu 93 5 not not RB cord-341342-kyavg4vu 93 6 bear bear VB cord-341342-kyavg4vu 93 7 on on IN cord-341342-kyavg4vu 93 8 the the DT cord-341342-kyavg4vu 93 9 present present JJ cord-341342-kyavg4vu 93 10 study study NN cord-341342-kyavg4vu 93 11 . . . cord-341342-kyavg4vu 94 1 To to TO cord-341342-kyavg4vu 94 2 examine examine VB cord-341342-kyavg4vu 94 3 the the DT cord-341342-kyavg4vu 94 4 properties property NNS cord-341342-kyavg4vu 94 5 of of IN cord-341342-kyavg4vu 94 6 native native JJ cord-341342-kyavg4vu 94 7 N n CD cord-341342-kyavg4vu 94 8 protein protein NN cord-341342-kyavg4vu 94 9 , , , cord-341342-kyavg4vu 94 10 the the DT cord-341342-kyavg4vu 94 11 in in FW cord-341342-kyavg4vu 94 12 vitro vitro FW cord-341342-kyavg4vu 94 13 translation translation NN cord-341342-kyavg4vu 94 14 product product NN cord-341342-kyavg4vu 94 15 was be VBD cord-341342-kyavg4vu 94 16 analyzed analyze VBN cord-341342-kyavg4vu 94 17 by by IN cord-341342-kyavg4vu 94 18 nondenaturing nondenature VBG cord-341342-kyavg4vu 94 19 PAGE page NN cord-341342-kyavg4vu 94 20 . . . cord-341342-kyavg4vu 95 1 In in IN cord-341342-kyavg4vu 95 2 this this DT cord-341342-kyavg4vu 95 3 gel gel NN cord-341342-kyavg4vu 95 4 electrophoresis electrophoresis NN cord-341342-kyavg4vu 95 5 system system NN cord-341342-kyavg4vu 95 6 , , , cord-341342-kyavg4vu 95 7 the the DT cord-341342-kyavg4vu 95 8 major major JJ cord-341342-kyavg4vu 95 9 fraction fraction NN cord-341342-kyavg4vu 95 10 ( ( -LRB- cord-341342-kyavg4vu 95 11 ca ca NN cord-341342-kyavg4vu 95 12 . . . cord-341342-kyavg4vu 96 1 60 60 CD cord-341342-kyavg4vu 96 2 % % NN cord-341342-kyavg4vu 96 3 ) ) -RRB- cord-341342-kyavg4vu 96 4 of of IN cord-341342-kyavg4vu 96 5 translated translate VBN cord-341342-kyavg4vu 96 6 N n CD cord-341342-kyavg4vu 96 7 protein protein NN cord-341342-kyavg4vu 96 8 migrated migrate VBD cord-341342-kyavg4vu 96 9 into into IN cord-341342-kyavg4vu 96 10 the the DT cord-341342-kyavg4vu 96 11 running running NN cord-341342-kyavg4vu 96 12 gel gel NN cord-341342-kyavg4vu 96 13 , , , cord-341342-kyavg4vu 96 14 forming form VBG cord-341342-kyavg4vu 96 15 a a DT cord-341342-kyavg4vu 96 16 discrete discrete JJ cord-341342-kyavg4vu 96 17 band band NN cord-341342-kyavg4vu 96 18 with with IN cord-341342-kyavg4vu 96 19 a a DT cord-341342-kyavg4vu 96 20 mobility mobility NN cord-341342-kyavg4vu 96 21 of of IN cord-341342-kyavg4vu 96 22 0.25 0.25 CD cord-341342-kyavg4vu 96 23 relative relative NN cord-341342-kyavg4vu 96 24 to to IN cord-341342-kyavg4vu 96 25 bromphenol bromphenol NNP cord-341342-kyavg4vu 96 26 blue blue JJ cord-341342-kyavg4vu 96 27 ( ( -LRB- cord-341342-kyavg4vu 96 28 Fig fig NN cord-341342-kyavg4vu 96 29 . . . cord-341342-kyavg4vu 96 30 2 2 CD cord-341342-kyavg4vu 96 31 B b NN cord-341342-kyavg4vu 96 32 , , , cord-341342-kyavg4vu 96 33 lane lane NNP cord-341342-kyavg4vu 96 34 f f NNP cord-341342-kyavg4vu 96 35 ) ) -RRB- cord-341342-kyavg4vu 96 36 . . . cord-341342-kyavg4vu 97 1 The the DT cord-341342-kyavg4vu 97 2 MHV MHV NNP cord-341342-kyavg4vu 97 3 - - HYPH cord-341342-kyavg4vu 97 4 A59 A59 NNP cord-341342-kyavg4vu 97 5 N N NNP cord-341342-kyavg4vu 97 6 protein protein NN cord-341342-kyavg4vu 97 7 contains contain VBZ cord-341342-kyavg4vu 97 8 a a DT cord-341342-kyavg4vu 97 9 considerable considerable JJ cord-341342-kyavg4vu 97 10 excess excess NN cord-341342-kyavg4vu 97 11 of of IN cord-341342-kyavg4vu 97 12 basic basic JJ cord-341342-kyavg4vu 97 13 amino amino NN cord-341342-kyavg4vu 97 14 acid acid NN cord-341342-kyavg4vu 97 15 residues residue NNS cord-341342-kyavg4vu 97 16 ( ( -LRB- cord-341342-kyavg4vu 97 17 63 63 CD cord-341342-kyavg4vu 97 18 lysines lysine NNS cord-341342-kyavg4vu 97 19 and and CC cord-341342-kyavg4vu 97 20 arginines arginines NNP cord-341342-kyavg4vu 97 21 as as IN cord-341342-kyavg4vu 97 22 opposed oppose VBN cord-341342-kyavg4vu 97 23 to to IN cord-341342-kyavg4vu 97 24 46 46 CD cord-341342-kyavg4vu 97 25 glutamates glutamate NNS cord-341342-kyavg4vu 97 26 and and CC cord-341342-kyavg4vu 97 27 aspartates aspartates NNPS cord-341342-kyavg4vu 97 28 ) ) -RRB- cord-341342-kyavg4vu 97 29 and and CC cord-341342-kyavg4vu 97 30 has have VBZ cord-341342-kyavg4vu 97 31 a a DT cord-341342-kyavg4vu 97 32 calculated calculate VBN cord-341342-kyavg4vu 97 33 pI pi NN cord-341342-kyavg4vu 97 34 of of IN cord-341342-kyavg4vu 97 35 10.5 10.5 CD cord-341342-kyavg4vu 97 36 [ [ -LRB- cord-341342-kyavg4vu 97 37 20 20 CD cord-341342-kyavg4vu 97 38 ] ] -RRB- cord-341342-kyavg4vu 97 39 . . . cord-341342-kyavg4vu 98 1 Initially initially RB cord-341342-kyavg4vu 98 2 it -PRON- PRP cord-341342-kyavg4vu 98 3 was be VBD cord-341342-kyavg4vu 98 4 surprising surprising JJ cord-341342-kyavg4vu 98 5 that that IN cord-341342-kyavg4vu 98 6 such such PDT cord-341342-kyavg4vu 98 7 a a DT cord-341342-kyavg4vu 98 8 molecule molecule NN cord-341342-kyavg4vu 98 9 , , , cord-341342-kyavg4vu 98 10 expected expect VBN cord-341342-kyavg4vu 98 11 to to TO cord-341342-kyavg4vu 98 12 have have VB cord-341342-kyavg4vu 98 13 a a DT cord-341342-kyavg4vu 98 14 substantial substantial JJ cord-341342-kyavg4vu 98 15 net net JJ cord-341342-kyavg4vu 98 16 positive positive JJ cord-341342-kyavg4vu 98 17 charge charge NN cord-341342-kyavg4vu 98 18 , , , cord-341342-kyavg4vu 98 19 was be VBD cord-341342-kyavg4vu 98 20 migrating migrate VBG cord-341342-kyavg4vu 98 21 toward toward IN cord-341342-kyavg4vu 98 22 the the DT cord-341342-kyavg4vu 98 23 positive positive JJ cord-341342-kyavg4vu 98 24 pole pole NN cord-341342-kyavg4vu 98 25 during during IN cord-341342-kyavg4vu 98 26 electrophoresis electrophoresis NN cord-341342-kyavg4vu 98 27 . . . cord-341342-kyavg4vu 99 1 This this DT cord-341342-kyavg4vu 99 2 result result NN cord-341342-kyavg4vu 99 3 suggested suggest VBD cord-341342-kyavg4vu 99 4 that that IN cord-341342-kyavg4vu 99 5 the the DT cord-341342-kyavg4vu 99 6 N N NNP cord-341342-kyavg4vu 99 7 molecule molecule NN cord-341342-kyavg4vu 99 8 was be VBD cord-341342-kyavg4vu 99 9 tightly tightly RB cord-341342-kyavg4vu 99 10 bound bind VBN cord-341342-kyavg4vu 99 11 to to IN cord-341342-kyavg4vu 99 12 some some DT cord-341342-kyavg4vu 99 13 polyanionic polyanionic JJ cord-341342-kyavg4vu 99 14 molecule molecule NN cord-341342-kyavg4vu 99 15 and and CC cord-341342-kyavg4vu 99 16 migrated migrate VBD cord-341342-kyavg4vu 99 17 as as IN cord-341342-kyavg4vu 99 18 part part NN cord-341342-kyavg4vu 99 19 of of IN cord-341342-kyavg4vu 99 20 a a DT cord-341342-kyavg4vu 99 21 complex complex NN cord-341342-kyavg4vu 99 22 having have VBG cord-341342-kyavg4vu 99 23 an an DT cord-341342-kyavg4vu 99 24 overall overall JJ cord-341342-kyavg4vu 99 25 negative negative JJ cord-341342-kyavg4vu 99 26 charge charge NN cord-341342-kyavg4vu 99 27 . . . cord-341342-kyavg4vu 100 1 That that IN cord-341342-kyavg4vu 100 2 this this DT cord-341342-kyavg4vu 100 3 , , , cord-341342-kyavg4vu 100 4 in in IN cord-341342-kyavg4vu 100 5 fact fact NN cord-341342-kyavg4vu 100 6 , , , cord-341342-kyavg4vu 100 7 had have VBD cord-341342-kyavg4vu 100 8 occurred occur VBN cord-341342-kyavg4vu 100 9 was be VBD cord-341342-kyavg4vu 100 10 shown show VBN cord-341342-kyavg4vu 100 11 by by IN cord-341342-kyavg4vu 100 12 incubation incubation NN cord-341342-kyavg4vu 100 13 of of IN cord-341342-kyavg4vu 100 14 translated translate VBN cord-341342-kyavg4vu 100 15 N n CD cord-341342-kyavg4vu 100 16 protein protein NN cord-341342-kyavg4vu 100 17 with with IN cord-341342-kyavg4vu 100 18 RNase RNase NNP cord-341342-kyavg4vu 100 19 A A NNP cord-341342-kyavg4vu 100 20 prior prior RB cord-341342-kyavg4vu 100 21 to to IN cord-341342-kyavg4vu 100 22 nondenaturing nondenature VBG cord-341342-kyavg4vu 100 23 gel gel NN cord-341342-kyavg4vu 100 24 electrophoresis electrophoresis NN cord-341342-kyavg4vu 100 25 . . . cord-341342-kyavg4vu 101 1 RNase rnase NN cord-341342-kyavg4vu 101 2 treatment treatment NN cord-341342-kyavg4vu 101 3 abolished abolish VBD cord-341342-kyavg4vu 101 4 the the DT cord-341342-kyavg4vu 101 5 ability ability NN cord-341342-kyavg4vu 101 6 of of IN cord-341342-kyavg4vu 101 7 translated translate VBN cord-341342-kyavg4vu 101 8 N n CD cord-341342-kyavg4vu 101 9 protein protein NN cord-341342-kyavg4vu 101 10 to to TO cord-341342-kyavg4vu 101 11 migrate migrate VB cord-341342-kyavg4vu 101 12 into into IN cord-341342-kyavg4vu 101 13 the the DT cord-341342-kyavg4vu 101 14 nondenaturing nondenature VBG cord-341342-kyavg4vu 101 15 gel gel NN cord-341342-kyavg4vu 101 16 ( ( -LRB- cord-341342-kyavg4vu 101 17 Fig Fig NNP cord-341342-kyavg4vu 101 18 . . . cord-341342-kyavg4vu 101 19 2 2 CD cord-341342-kyavg4vu 101 20 B b NN cord-341342-kyavg4vu 101 21 , , , cord-341342-kyavg4vu 101 22 lane lane NN cord-341342-kyavg4vu 101 23 g g NNP cord-341342-kyavg4vu 101 24 ) ) -RRB- cord-341342-kyavg4vu 101 25 . . . cord-341342-kyavg4vu 102 1 Thus thus RB cord-341342-kyavg4vu 102 2 , , , cord-341342-kyavg4vu 102 3 synthesized synthesize VBN cord-341342-kyavg4vu 102 4 N n NN cord-341342-kyavg4vu 102 5 protein protein NN cord-341342-kyavg4vu 102 6 had have VBD cord-341342-kyavg4vu 102 7 bound bind VBN cord-341342-kyavg4vu 102 8 to to IN cord-341342-kyavg4vu 102 9 some some DT cord-341342-kyavg4vu 102 10 RNA RNA NNP cord-341342-kyavg4vu 102 11 species specie NNS cord-341342-kyavg4vu 102 12 present present JJ cord-341342-kyavg4vu 102 13 in in IN cord-341342-kyavg4vu 102 14 the the DT cord-341342-kyavg4vu 102 15 translation translation NN cord-341342-kyavg4vu 102 16 reaction reaction NN cord-341342-kyavg4vu 102 17 , , , cord-341342-kyavg4vu 102 18 and and CC cord-341342-kyavg4vu 102 19 as as IN cord-341342-kyavg4vu 102 20 a a DT cord-341342-kyavg4vu 102 21 consequence consequence NN cord-341342-kyavg4vu 102 22 of of IN cord-341342-kyavg4vu 102 23 this this DT cord-341342-kyavg4vu 102 24 complex complex JJ cord-341342-kyavg4vu 102 25 formation formation NN cord-341342-kyavg4vu 102 26 it -PRON- PRP cord-341342-kyavg4vu 102 27 was be VBD cord-341342-kyavg4vu 102 28 carried carry VBN cord-341342-kyavg4vu 102 29 into into IN cord-341342-kyavg4vu 102 30 the the DT cord-341342-kyavg4vu 102 31 nondenaturing nondenature VBG cord-341342-kyavg4vu 102 32 gel gel NN cord-341342-kyavg4vu 102 33 during during IN cord-341342-kyavg4vu 102 34 electrophoresis electrophoresis NN cord-341342-kyavg4vu 102 35 . . . cord-341342-kyavg4vu 103 1 N N NNP cord-341342-kyavg4vu 103 2 protein protein NN cord-341342-kyavg4vu 103 3 translated translate VBD cord-341342-kyavg4vu 103 4 from from IN cord-341342-kyavg4vu 103 5 total total JJ cord-341342-kyavg4vu 103 6 RNA RNA NNP cord-341342-kyavg4vu 103 7 purified purify VBN cord-341342-kyavg4vu 103 8 from from IN cord-341342-kyavg4vu 103 9 MHV MHV NNP cord-341342-kyavg4vu 103 10 - - HYPH cord-341342-kyavg4vu 103 11 A59-infected a59-infecte VBN cord-341342-kyavg4vu 103 12 cells cell NNS cord-341342-kyavg4vu 103 13 behaved behave VBD cord-341342-kyavg4vu 103 14 in in IN cord-341342-kyavg4vu 103 15 the the DT cord-341342-kyavg4vu 103 16 same same JJ cord-341342-kyavg4vu 103 17 RNase rnase NN cord-341342-kyavg4vu 103 18 - - HYPH cord-341342-kyavg4vu 103 19 sensitive sensitive JJ cord-341342-kyavg4vu 103 20 manner manner NN cord-341342-kyavg4vu 104 1 ( ( -LRB- cord-341342-kyavg4vu 104 2 Fig Fig NNP cord-341342-kyavg4vu 104 3 . . . cord-341342-kyavg4vu 104 4 2 2 CD cord-341342-kyavg4vu 104 5 more more RBR cord-341342-kyavg4vu 104 6 immediately immediately RB cord-341342-kyavg4vu 104 7 digested digest VBN cord-341342-kyavg4vu 104 8 . . . cord-341342-kyavg4vu 105 1 The the DT cord-341342-kyavg4vu 105 2 N n CD cord-341342-kyavg4vu 105 3 -R -r ADD cord-341342-kyavg4vu 106 1 N N NNP cord-341342-kyavg4vu 106 2 A a DT cord-341342-kyavg4vu 106 3 complex complex NN cord-341342-kyavg4vu 106 4 was be VBD cord-341342-kyavg4vu 106 5 more more RBR cord-341342-kyavg4vu 106 6 sensitive sensitive JJ cord-341342-kyavg4vu 106 7 to to IN cord-341342-kyavg4vu 106 8 RNase RNase NNP cord-341342-kyavg4vu 106 9 A a DT cord-341342-kyavg4vu 106 10 than than IN cord-341342-kyavg4vu 106 11 to to IN cord-341342-kyavg4vu 106 12 RNase RNase NNP cord-341342-kyavg4vu 106 13 T1 T1 NNP cord-341342-kyavg4vu 106 14 , , , cord-341342-kyavg4vu 106 15 possibly possibly RB cord-341342-kyavg4vu 106 16 due due IN cord-341342-kyavg4vu 106 17 to to IN cord-341342-kyavg4vu 106 18 the the DT cord-341342-kyavg4vu 106 19 different different JJ cord-341342-kyavg4vu 106 20 nucleotide nucleotide JJ cord-341342-kyavg4vu 106 21 specificities specificity NNS cord-341342-kyavg4vu 106 22 of of IN cord-341342-kyavg4vu 106 23 these these DT cord-341342-kyavg4vu 106 24 enzymes enzyme NNS cord-341342-kyavg4vu 107 1 ( ( -LRB- cord-341342-kyavg4vu 107 2 RNase RNase NNP cord-341342-kyavg4vu 107 3 A A NNP cord-341342-kyavg4vu 107 4 cuts cut VBZ cord-341342-kyavg4vu 107 5 3 3 CD cord-341342-kyavg4vu 107 6 ' ' '' cord-341342-kyavg4vu 107 7 to to IN cord-341342-kyavg4vu 107 8 pyrimidines pyrimidine NNS cord-341342-kyavg4vu 107 9 ; ; : cord-341342-kyavg4vu 107 10 RNase RNase NNP cord-341342-kyavg4vu 107 11 T1 T1 NNP cord-341342-kyavg4vu 107 12 cuts cut VBZ cord-341342-kyavg4vu 107 13 3 3 CD cord-341342-kyavg4vu 107 14 ' ' '' cord-341342-kyavg4vu 107 15 to to IN cord-341342-kyavg4vu 107 16 G g NN cord-341342-kyavg4vu 107 17 residues residue NNS cord-341342-kyavg4vu 107 18 ) ) -RRB- cord-341342-kyavg4vu 107 19 . . . cord-341342-kyavg4vu 108 1 Alternatively alternatively RB cord-341342-kyavg4vu 108 2 , , , cord-341342-kyavg4vu 108 3 the the DT cord-341342-kyavg4vu 108 4 differential differential JJ cord-341342-kyavg4vu 108 5 sensitivity sensitivity NN cord-341342-kyavg4vu 108 6 may may MD cord-341342-kyavg4vu 108 7 have have VB cord-341342-kyavg4vu 108 8 reflected reflect VBN cord-341342-kyavg4vu 108 9 the the DT cord-341342-kyavg4vu 108 10 relative relative JJ cord-341342-kyavg4vu 108 11 accessibility accessibility NN cord-341342-kyavg4vu 108 12 of of IN cord-341342-kyavg4vu 108 13 each each DT cord-341342-kyavg4vu 108 14 enzyme enzyme NN cord-341342-kyavg4vu 108 15 to to IN cord-341342-kyavg4vu 108 16 phosphodiester phosphodiester NN cord-341342-kyavg4vu 108 17 bonds bond NNS cord-341342-kyavg4vu 108 18 within within IN cord-341342-kyavg4vu 108 19 the the DT cord-341342-kyavg4vu 108 20 N N NNP cord-341342-kyavg4vu 108 21 -R -R NNP cord-341342-kyavg4vu 108 22 N N NNP cord-341342-kyavg4vu 108 23 A A NNP cord-341342-kyavg4vu 108 24 complex complex NN cord-341342-kyavg4vu 108 25 . . . cord-341342-kyavg4vu 109 1 The the DT cord-341342-kyavg4vu 109 2 N n CD cord-341342-kyavg4vu 109 3 proteins protein NNS cord-341342-kyavg4vu 109 4 of of IN cord-341342-kyavg4vu 109 5 coronaviruses coronaviruse NNS cord-341342-kyavg4vu 109 6 provide provide VBP cord-341342-kyavg4vu 109 7 their -PRON- PRP$ cord-341342-kyavg4vu 109 8 encapsidated encapsidate VBN cord-341342-kyavg4vu 109 9 genomes genome NNS cord-341342-kyavg4vu 109 10 only only RB cord-341342-kyavg4vu 109 11 a a DT cord-341342-kyavg4vu 109 12 minimal minimal JJ cord-341342-kyavg4vu 109 13 degree degree NN cord-341342-kyavg4vu 109 14 of of IN cord-341342-kyavg4vu 109 15 protection protection NN cord-341342-kyavg4vu 109 16 against against IN cord-341342-kyavg4vu 109 17 the the DT cord-341342-kyavg4vu 109 18 action action NN cord-341342-kyavg4vu 109 19 of of IN cord-341342-kyavg4vu 109 20 ribonucleases ribonuclease NNS cord-341342-kyavg4vu 109 21 [ [ -LRB- cord-341342-kyavg4vu 109 22 12 12 CD cord-341342-kyavg4vu 109 23 , , , cord-341342-kyavg4vu 109 24 22 22 CD cord-341342-kyavg4vu 109 25 ] ] -RRB- cord-341342-kyavg4vu 109 26 . . . cord-341342-kyavg4vu 110 1 Hence hence RB cord-341342-kyavg4vu 110 2 , , , cord-341342-kyavg4vu 110 3 in in IN cord-341342-kyavg4vu 110 4 this this DT cord-341342-kyavg4vu 110 5 respect respect NN cord-341342-kyavg4vu 110 6 , , , cord-341342-kyavg4vu 110 7 the the DT cord-341342-kyavg4vu 110 8 N N NNP cord-341342-kyavg4vu 110 9 -R -R NNP cord-341342-kyavg4vu 110 10 N N NNP cord-341342-kyavg4vu 110 11 A a DT cord-341342-kyavg4vu 110 12 interaction interaction NN cord-341342-kyavg4vu 110 13 in in IN cord-341342-kyavg4vu 110 14 the the DT cord-341342-kyavg4vu 110 15 nondenaturing nondenature VBG cord-341342-kyavg4vu 110 16 gel gel NN cord-341342-kyavg4vu 110 17 assay assay NN cord-341342-kyavg4vu 110 18 resembled resemble VBD cord-341342-kyavg4vu 110 19 that that WDT cord-341342-kyavg4vu 110 20 found find VBN cord-341342-kyavg4vu 110 21 in in IN cord-341342-kyavg4vu 110 22 the the DT cord-341342-kyavg4vu 110 23 M M NNP cord-341342-kyavg4vu 110 24 H h NN cord-341342-kyavg4vu 110 25 V v NN cord-341342-kyavg4vu 110 26 nucleocapsid nucleocapsid NN cord-341342-kyavg4vu 110 27 . . . cord-341342-kyavg4vu 111 1 The the DT cord-341342-kyavg4vu 111 2 RNase rnase NN cord-341342-kyavg4vu 111 3 sensitivity sensitivity NN cord-341342-kyavg4vu 111 4 of of IN cord-341342-kyavg4vu 111 5 the the DT cord-341342-kyavg4vu 111 6 ability ability NN cord-341342-kyavg4vu 111 7 of of IN cord-341342-kyavg4vu 111 8 N n CD cord-341342-kyavg4vu 111 9 protein protein NN cord-341342-kyavg4vu 111 10 to to TO cord-341342-kyavg4vu 111 11 migrate migrate VB cord-341342-kyavg4vu 111 12 into into IN cord-341342-kyavg4vu 111 13 nondenaturing nondenature VBG cord-341342-kyavg4vu 111 14 gels gel NNS cord-341342-kyavg4vu 111 15 was be VBD cord-341342-kyavg4vu 111 16 not not RB cord-341342-kyavg4vu 111 17 due due JJ cord-341342-kyavg4vu 111 18 to to IN cord-341342-kyavg4vu 111 19 possible possible JJ cord-341342-kyavg4vu 111 20 contaminating contaminate VBG cord-341342-kyavg4vu 111 21 protease protease NN cord-341342-kyavg4vu 111 22 activity activity NN cord-341342-kyavg4vu 111 23 in in IN cord-341342-kyavg4vu 111 24 the the DT cord-341342-kyavg4vu 111 25 RNase rnase NN cord-341342-kyavg4vu 111 26 preparations preparation NNS cord-341342-kyavg4vu 111 27 , , , cord-341342-kyavg4vu 111 28 since since IN cord-341342-kyavg4vu 111 29 the the DT cord-341342-kyavg4vu 111 30 same same JJ cord-341342-kyavg4vu 111 31 RNase rnase NN cord-341342-kyavg4vu 111 32 - - HYPH cord-341342-kyavg4vu 111 33 treated treat VBN cord-341342-kyavg4vu 111 34 samples sample NNS cord-341342-kyavg4vu 111 35 of of IN cord-341342-kyavg4vu 111 36 translated translate VBN cord-341342-kyavg4vu 111 37 N n CD cord-341342-kyavg4vu 111 38 148 148 CD cord-341342-kyavg4vu 111 39 P.S. P.S. NNP cord-341342-kyavg4vu 111 40 Masters Masters NNP cord-341342-kyavg4vu 111 41 protein protein NN cord-341342-kyavg4vu 111 42 showed show VBD cord-341342-kyavg4vu 111 43 no no DT cord-341342-kyavg4vu 111 44 degradation degradation NN cord-341342-kyavg4vu 111 45 when when WRB cord-341342-kyavg4vu 111 46 analyzed analyze VBN cord-341342-kyavg4vu 111 47 by by IN cord-341342-kyavg4vu 111 48 SDS SDS NNP cord-341342-kyavg4vu 111 49 - - HYPH cord-341342-kyavg4vu 111 50 PAGE PAGE NNP cord-341342-kyavg4vu 111 51 ( ( -LRB- cord-341342-kyavg4vu 111 52 Fig Fig NNP cord-341342-kyavg4vu 111 53 . . . cord-341342-kyavg4vu 111 54 3 3 CD cord-341342-kyavg4vu 111 55 , , , cord-341342-kyavg4vu 111 56 lanes lane VBZ cord-341342-kyavg4vu 111 57 an an DT cord-341342-kyavg4vu 111 58 , , , cord-341342-kyavg4vu 111 59 lower low JJR cord-341342-kyavg4vu 111 60 ) ) -RRB- cord-341342-kyavg4vu 111 61 . . . cord-341342-kyavg4vu 112 1 Moreover moreover RB cord-341342-kyavg4vu 112 2 , , , cord-341342-kyavg4vu 112 3 inclusion inclusion NN cord-341342-kyavg4vu 112 4 of of IN cord-341342-kyavg4vu 112 5 the the DT cord-341342-kyavg4vu 112 6 placental placental NN cord-341342-kyavg4vu 113 1 RNase RNase NNP cord-341342-kyavg4vu 113 2 A a DT cord-341342-kyavg4vu 113 3 inhibitor inhibitor NN cord-341342-kyavg4vu 113 4 , , , cord-341342-kyavg4vu 113 5 RNasin RNasin NNP cord-341342-kyavg4vu 113 6 , , , cord-341342-kyavg4vu 113 7 allowed allow VBN cord-341342-kyavg4vu 113 8 at at IN cord-341342-kyavg4vu 113 9 least least JJS cord-341342-kyavg4vu 113 10 partial partial JJ cord-341342-kyavg4vu 113 11 inhibition inhibition NN cord-341342-kyavg4vu 113 12 of of IN cord-341342-kyavg4vu 113 13 the the DT cord-341342-kyavg4vu 113 14 effect effect NN cord-341342-kyavg4vu 113 15 of of IN cord-341342-kyavg4vu 113 16 an an DT cord-341342-kyavg4vu 113 17 intermediate intermediate JJ cord-341342-kyavg4vu 113 18 concentration concentration NN cord-341342-kyavg4vu 113 19 of of IN cord-341342-kyavg4vu 113 20 RNase RNase NNP cord-341342-kyavg4vu 113 21 A A NNP cord-341342-kyavg4vu 113 22 ( ( -LRB- cord-341342-kyavg4vu 113 23 Fig Fig NNP cord-341342-kyavg4vu 113 24 . . . cord-341342-kyavg4vu 113 25 3 3 CD cord-341342-kyavg4vu 113 26 , , , cord-341342-kyavg4vu 113 27 lanes lane NNS cord-341342-kyavg4vu 113 28 p p NN cord-341342-kyavg4vu 113 29 and and CC cord-341342-kyavg4vu 113 30 q q NNP cord-341342-kyavg4vu 113 31 ) ) -RRB- cord-341342-kyavg4vu 113 32 . . . cord-341342-kyavg4vu 114 1 Incubation incubation NN cord-341342-kyavg4vu 114 2 with with IN cord-341342-kyavg4vu 114 3 RNase rnase NN cord-341342-kyavg4vu 114 4 - - HYPH cord-341342-kyavg4vu 114 5 free free JJ cord-341342-kyavg4vu 114 6 DNase DNase NNP cord-341342-kyavg4vu 114 7 had have VBD cord-341342-kyavg4vu 114 8 no no DT cord-341342-kyavg4vu 114 9 effect effect NN cord-341342-kyavg4vu 114 10 on on IN cord-341342-kyavg4vu 114 11 the the DT cord-341342-kyavg4vu 114 12 mobility mobility NN cord-341342-kyavg4vu 114 13 of of IN cord-341342-kyavg4vu 114 14 N n CD cord-341342-kyavg4vu 114 15 protein protein NN cord-341342-kyavg4vu 114 16 in in IN cord-341342-kyavg4vu 114 17 nondenaturing nondenature VBG cord-341342-kyavg4vu 114 18 PAGE page NN cord-341342-kyavg4vu 114 19 ( ( -LRB- cord-341342-kyavg4vu 114 20 Fig Fig NNP cord-341342-kyavg4vu 114 21 . . . cord-341342-kyavg4vu 114 22 3 3 CD cord-341342-kyavg4vu 114 23 , , , cord-341342-kyavg4vu 114 24 lane lane NN cord-341342-kyavg4vu 114 25 r r NN cord-341342-kyavg4vu 114 26 ) ) -RRB- cord-341342-kyavg4vu 114 27 . . . cord-341342-kyavg4vu 115 1 It -PRON- PRP cord-341342-kyavg4vu 115 2 has have VBZ cord-341342-kyavg4vu 115 3 been be VBN cord-341342-kyavg4vu 115 4 reported report VBN cord-341342-kyavg4vu 115 5 previously previously RB cord-341342-kyavg4vu 115 6 that that IN cord-341342-kyavg4vu 115 7 the the DT cord-341342-kyavg4vu 115 8 MHV MHV NNP cord-341342-kyavg4vu 115 9 N N NNP cord-341342-kyavg4vu 115 10 protein protein NN cord-341342-kyavg4vu 115 11 exhibits exhibit VBZ cord-341342-kyavg4vu 115 12 sequencespecific sequencespecific NNP cord-341342-kyavg4vu 115 13 binding bind VBG cord-341342-kyavg4vu 115 14 to to IN cord-341342-kyavg4vu 115 15 nucleotides nucleotide NNS cord-341342-kyavg4vu 115 16 56 56 CD cord-341342-kyavg4vu 115 17 to to TO cord-341342-kyavg4vu 115 18 65 65 CD cord-341342-kyavg4vu 115 19 of of IN cord-341342-kyavg4vu 115 20 the the DT cord-341342-kyavg4vu 115 21 MHV MHV NNP cord-341342-kyavg4vu 115 22 leader leader NN cord-341342-kyavg4vu 115 23 RNA RNA NNP cord-341342-kyavg4vu 115 24 [ [ -LRB- cord-341342-kyavg4vu 115 25 29 29 CD cord-341342-kyavg4vu 115 26 ] ] -RRB- cord-341342-kyavg4vu 115 27 . . . cord-341342-kyavg4vu 116 1 Since since IN cord-341342-kyavg4vu 116 2 the the DT cord-341342-kyavg4vu 116 3 N n NN cord-341342-kyavg4vu 116 4 mRNAs mRNAs NNPS cord-341342-kyavg4vu 116 5 synthesized synthesize VBN cord-341342-kyavg4vu 116 6 from from IN cord-341342-kyavg4vu 116 7 the the DT cord-341342-kyavg4vu 116 8 transcription transcription NN cord-341342-kyavg4vu 116 9 vectors vector NNS cord-341342-kyavg4vu 116 10 shown show VBN cord-341342-kyavg4vu 116 11 in in IN cord-341342-kyavg4vu 116 12 Fig Fig NNP cord-341342-kyavg4vu 116 13 . . . cord-341342-kyavg4vu 117 1 1 1 CD cord-341342-kyavg4vu 117 2 contained contain VBD cord-341342-kyavg4vu 117 3 this this DT cord-341342-kyavg4vu 117 4 portion portion NN cord-341342-kyavg4vu 117 5 of of IN cord-341342-kyavg4vu 117 6 the the DT cord-341342-kyavg4vu 117 7 leader leader NN cord-341342-kyavg4vu 117 8 sequence sequence NN cord-341342-kyavg4vu 117 9 , , , cord-341342-kyavg4vu 117 10 it -PRON- PRP cord-341342-kyavg4vu 117 11 seemed seem VBD cord-341342-kyavg4vu 117 12 possible possible JJ cord-341342-kyavg4vu 117 13 that that IN cord-341342-kyavg4vu 117 14 the the DT cord-341342-kyavg4vu 117 15 N N NNP cord-341342-kyavg4vu 117 16 - - HYPH cord-341342-kyavg4vu 117 17 RNA RNA NNP cord-341342-kyavg4vu 117 18 complex complex NN cord-341342-kyavg4vu 117 19 observed observe VBD cord-341342-kyavg4vu 117 20 in in IN cord-341342-kyavg4vu 117 21 nondenaturing nondenature VBG cord-341342-kyavg4vu 117 22 PAGE PAGE NNP cord-341342-kyavg4vu 117 23 was be VBD cord-341342-kyavg4vu 117 24 that that DT cord-341342-kyavg4vu 117 25 of of IN cord-341342-kyavg4vu 117 26 translated translate VBN cord-341342-kyavg4vu 117 27 N n CD cord-341342-kyavg4vu 117 28 protein protein NN cord-341342-kyavg4vu 117 29 binding bind VBG cord-341342-kyavg4vu 117 30 to to IN cord-341342-kyavg4vu 117 31 its -PRON- PRP$ cord-341342-kyavg4vu 117 32 own own JJ cord-341342-kyavg4vu 117 33 mRNA mrna NN cord-341342-kyavg4vu 117 34 . . . cord-341342-kyavg4vu 118 1 To to TO cord-341342-kyavg4vu 118 2 test test VB cord-341342-kyavg4vu 118 3 this this DT cord-341342-kyavg4vu 118 4 notion notion NN cord-341342-kyavg4vu 118 5 , , , cord-341342-kyavg4vu 118 6 parallel parallel JJ cord-341342-kyavg4vu 118 7 translation translation NN cord-341342-kyavg4vu 118 8 reactions reaction NNS cord-341342-kyavg4vu 118 9 were be VBD cord-341342-kyavg4vu 118 10 carried carry VBN cord-341342-kyavg4vu 118 11 out out RP cord-341342-kyavg4vu 118 12 either either CC cord-341342-kyavg4vu 118 13 with with IN cord-341342-kyavg4vu 118 14 unlabeled unlabeled JJ cord-341342-kyavg4vu 118 15 N N NNP cord-341342-kyavg4vu 118 16 mRNA mrna NN cord-341342-kyavg4vu 118 17 or or CC cord-341342-kyavg4vu 118 18 with with IN cord-341342-kyavg4vu 118 19 N n CD cord-341342-kyavg4vu 118 20 mRNA mrna NN cord-341342-kyavg4vu 119 1 labeled label VBN cord-341342-kyavg4vu 119 2 to to IN cord-341342-kyavg4vu 119 3 high high JJ cord-341342-kyavg4vu 119 4 specific specific JJ cord-341342-kyavg4vu 119 5 activity activity NN cord-341342-kyavg4vu 119 6 ( ( -LRB- cord-341342-kyavg4vu 119 7 2.4 2.4 CD cord-341342-kyavg4vu 119 8 x x SYM cord-341342-kyavg4vu 119 9 10 10 CD cord-341342-kyavg4vu 119 10 7 7 CD cord-341342-kyavg4vu 119 11 cpm cpm NN cord-341342-kyavg4vu 119 12 / / SYM cord-341342-kyavg4vu 119 13 gg gg NNP cord-341342-kyavg4vu 119 14 ) ) -RRB- cord-341342-kyavg4vu 119 15 with with IN cord-341342-kyavg4vu 119 16 [ [ -LRB- cord-341342-kyavg4vu 119 17 a-32p]UTP a-32p]UTP NNP cord-341342-kyavg4vu 119 18 . . . cord-341342-kyavg4vu 120 1 N N NNP cord-341342-kyavg4vu 120 2 protein protein NN cord-341342-kyavg4vu 120 3 translated translate VBD cord-341342-kyavg4vu 120 4 from from IN cord-341342-kyavg4vu 120 5 unlabeled unlabeled JJ cord-341342-kyavg4vu 120 6 mRNA mrna NN cord-341342-kyavg4vu 120 7 was be VBD cord-341342-kyavg4vu 120 8 labeled label VBN cord-341342-kyavg4vu 120 9 with with IN cord-341342-kyavg4vu 120 10 [ [ -LRB- cord-341342-kyavg4vu 120 11 35S]methionine 35s]methionine CD cord-341342-kyavg4vu 120 12 , , , cord-341342-kyavg4vu 120 13 while while IN cord-341342-kyavg4vu 120 14 N n CD cord-341342-kyavg4vu 120 15 protein protein NN cord-341342-kyavg4vu 120 16 translated translate VBD cord-341342-kyavg4vu 120 17 from from IN cord-341342-kyavg4vu 120 18 labeled label VBN cord-341342-kyavg4vu 120 19 mRNA mrna NN cord-341342-kyavg4vu 120 20 was be VBD cord-341342-kyavg4vu 120 21 left leave VBN cord-341342-kyavg4vu 120 22 unlabeled unlabeled JJ cord-341342-kyavg4vu 120 23 ( ( -LRB- cord-341342-kyavg4vu 120 24 Fig fig NN cord-341342-kyavg4vu 120 25 . . . cord-341342-kyavg4vu 120 26 4 4 CD cord-341342-kyavg4vu 120 27 ) ) -RRB- cord-341342-kyavg4vu 120 28 . . . cord-341342-kyavg4vu 121 1 It -PRON- PRP cord-341342-kyavg4vu 121 2 was be VBD cord-341342-kyavg4vu 121 3 expected expect VBN cord-341342-kyavg4vu 121 4 that that IN cord-341342-kyavg4vu 121 5 if if IN cord-341342-kyavg4vu 121 6 translated translate VBN cord-341342-kyavg4vu 121 7 N n CD cord-341342-kyavg4vu 121 8 protein protein NN cord-341342-kyavg4vu 121 9 were be VBD cord-341342-kyavg4vu 121 10 binding bind VBG cord-341342-kyavg4vu 121 11 to to IN cord-341342-kyavg4vu 121 12 its -PRON- PRP$ cord-341342-kyavg4vu 121 13 own own JJ cord-341342-kyavg4vu 121 14 mRNA mrna NN cord-341342-kyavg4vu 121 15 , , , cord-341342-kyavg4vu 121 16 then then RB cord-341342-kyavg4vu 121 17 a a DT cord-341342-kyavg4vu 121 18 32p 32p CD cord-341342-kyavg4vu 121 19 - - HYPH cord-341342-kyavg4vu 121 20 labeled label VBN cord-341342-kyavg4vu 121 21 RNA RNA NNP cord-341342-kyavg4vu 121 22 band band NN cord-341342-kyavg4vu 121 23 migrating migrate VBG cord-341342-kyavg4vu 121 24 to to IN cord-341342-kyavg4vu 121 25 the the DT cord-341342-kyavg4vu 121 26 same same JJ cord-341342-kyavg4vu 121 27 position position NN cord-341342-kyavg4vu 121 28 as as IN cord-341342-kyavg4vu 121 29 that that DT cord-341342-kyavg4vu 121 30 of of IN cord-341342-kyavg4vu 121 31 [ [ -LRB- cord-341342-kyavg4vu 121 32 35S]methionine 35s]methionine CD cord-341342-kyavg4vu 121 33 - - HYPH cord-341342-kyavg4vu 121 34 labeled label VBN cord-341342-kyavg4vu 121 35 N n NN cord-341342-kyavg4vu 121 36 protein protein NN cord-341342-kyavg4vu 121 37 should should MD cord-341342-kyavg4vu 121 38 have have VB cord-341342-kyavg4vu 121 39 been be VBN cord-341342-kyavg4vu 121 40 observed observe VBN cord-341342-kyavg4vu 121 41 in in IN cord-341342-kyavg4vu 121 42 nondenaturing nondenature VBG cord-341342-kyavg4vu 121 43 PAGE page NN cord-341342-kyavg4vu 121 44 . . . cord-341342-kyavg4vu 122 1 As as IN cord-341342-kyavg4vu 122 2 shown show VBN cord-341342-kyavg4vu 122 3 in in IN cord-341342-kyavg4vu 122 4 Fig Fig NNP cord-341342-kyavg4vu 122 5 . . . cord-341342-kyavg4vu 123 1 4 4 LS cord-341342-kyavg4vu 123 2 ( ( -LRB- cord-341342-kyavg4vu 123 3 lanes lane NNS cord-341342-kyavg4vu 123 4 b b NNP cord-341342-kyavg4vu 123 5 ' ' '' cord-341342-kyavg4vu 124 1 , , , cord-341342-kyavg4vu 124 2 c c NNP cord-341342-kyavg4vu 124 3 ' ' '' cord-341342-kyavg4vu 124 4 , , , cord-341342-kyavg4vu 124 5 f f NNP cord-341342-kyavg4vu 124 6 ' ' '' cord-341342-kyavg4vu 124 7 and and CC cord-341342-kyavg4vu 124 8 g g NNP cord-341342-kyavg4vu 124 9 ' ' '' cord-341342-kyavg4vu 124 10 ) ) -RRB- cord-341342-kyavg4vu 124 11 this this DT cord-341342-kyavg4vu 124 12 clearly clearly RB cord-341342-kyavg4vu 124 13 did do VBD cord-341342-kyavg4vu 124 14 not not RB cord-341342-kyavg4vu 124 15 occur occur VB cord-341342-kyavg4vu 124 16 . . . cord-341342-kyavg4vu 125 1 This this DT cord-341342-kyavg4vu 125 2 was be VBD cord-341342-kyavg4vu 125 3 not not RB cord-341342-kyavg4vu 125 4 due due JJ cord-341342-kyavg4vu 125 5 to to IN cord-341342-kyavg4vu 125 6 failure failure NN cord-341342-kyavg4vu 125 7 to to TO cord-341342-kyavg4vu 125 8 translate translate VB cord-341342-kyavg4vu 125 9 the the DT cord-341342-kyavg4vu 125 10 32p 32p CD cord-341342-kyavg4vu 125 11 - - HYPH cord-341342-kyavg4vu 125 12 labeled label VBN cord-341342-kyavg4vu 125 13 mRNA mrna NN cord-341342-kyavg4vu 125 14 , , , cord-341342-kyavg4vu 125 15 since since IN cord-341342-kyavg4vu 125 16 unlabeled unlabeled JJ cord-341342-kyavg4vu 125 17 and and CC cord-341342-kyavg4vu 125 18 labeled label VBN cord-341342-kyavg4vu 125 19 N n NN cord-341342-kyavg4vu 125 20 mRNAs mRNAs NNPS cord-341342-kyavg4vu 125 21 had have VBD cord-341342-kyavg4vu 125 22 comparable comparable JJ cord-341342-kyavg4vu 125 23 translation translation NN cord-341342-kyavg4vu 125 24 efficiencies efficiency NNS cord-341342-kyavg4vu 125 25 ( ( -LRB- cord-341342-kyavg4vu 125 26 Fig Fig NNP cord-341342-kyavg4vu 125 27 . . NNP cord-341342-kyavg4vu 125 28 4 4 CD cord-341342-kyavg4vu 125 29 , , , cord-341342-kyavg4vu 125 30 lanes lane NNS cord-341342-kyavg4vu 125 31 b b NNP cord-341342-kyavg4vu 125 32 , , , cord-341342-kyavg4vu 125 33 e e NNP cord-341342-kyavg4vu 125 34 , , , cord-341342-kyavg4vu 125 35 b b NNP cord-341342-kyavg4vu 125 36 ' ' '' cord-341342-kyavg4vu 125 37 , , , cord-341342-kyavg4vu 125 38 and and CC cord-341342-kyavg4vu 125 39 e e NNP cord-341342-kyavg4vu 125 40 ' ' '' cord-341342-kyavg4vu 125 41 ) ) -RRB- cord-341342-kyavg4vu 125 42 . . . cord-341342-kyavg4vu 126 1 During during IN cord-341342-kyavg4vu 126 2 the the DT cord-341342-kyavg4vu 126 3 course course NN cord-341342-kyavg4vu 126 4 of of IN cord-341342-kyavg4vu 126 5 the the DT cord-341342-kyavg4vu 126 6 90 90 CD cord-341342-kyavg4vu 126 7 min min NN cord-341342-kyavg4vu 126 8 translation translation NN cord-341342-kyavg4vu 126 9 reactions reaction NNS cord-341342-kyavg4vu 126 10 , , , cord-341342-kyavg4vu 126 11 the the DT cord-341342-kyavg4vu 126 12 32p 32p CD cord-341342-kyavg4vu 126 13 - - HYPH cord-341342-kyavg4vu 126 14 labeled label VBN cord-341342-kyavg4vu 126 15 mRNA mrna NN cord-341342-kyavg4vu 126 16 underwent undergo VBD cord-341342-kyavg4vu 126 17 limited limited JJ cord-341342-kyavg4vu 126 18 nucleolyfic nucleolyfic JJ cord-341342-kyavg4vu 126 19 degradation degradation NN cord-341342-kyavg4vu 126 20 , , , cord-341342-kyavg4vu 126 21 presumably presumably RB cord-341342-kyavg4vu 126 22 due due IN cord-341342-kyavg4vu 126 23 to to IN cord-341342-kyavg4vu 126 24 low low JJ cord-341342-kyavg4vu 126 25 levels level NNS cord-341342-kyavg4vu 126 26 of of IN cord-341342-kyavg4vu 126 27 RNase rnase NN cord-341342-kyavg4vu 126 28 activity activity NN cord-341342-kyavg4vu 126 29 in in IN cord-341342-kyavg4vu 126 30 the the DT cord-341342-kyavg4vu 126 31 reticulocyte reticulocyte NN cord-341342-kyavg4vu 126 32 lysate lysate NN cord-341342-kyavg4vu 126 33 . . . cord-341342-kyavg4vu 127 1 Most Most JJS cord-341342-kyavg4vu 127 2 of of IN cord-341342-kyavg4vu 127 3 the the DT cord-341342-kyavg4vu 127 4 32P 32p CD cord-341342-kyavg4vu 127 5 - - HYPH cord-341342-kyavg4vu 127 6 labeled label VBN cord-341342-kyavg4vu 127 7 material material NN cord-341342-kyavg4vu 127 8 produced produce VBN cord-341342-kyavg4vu 127 9 by by IN cord-341342-kyavg4vu 127 10 this this DT cord-341342-kyavg4vu 127 11 limited limited JJ cord-341342-kyavg4vu 127 12 breakdown breakdown NN cord-341342-kyavg4vu 127 13 was be VBD cord-341342-kyavg4vu 127 14 retained retain VBN cord-341342-kyavg4vu 127 15 by by IN cord-341342-kyavg4vu 127 16 SDS SDS NNP cord-341342-kyavg4vu 127 17 - - HYPH cord-341342-kyavg4vu 127 18 PAGE PAGE NNP cord-341342-kyavg4vu 127 19 , , , cord-341342-kyavg4vu 127 20 but but CC cord-341342-kyavg4vu 127 21 virtually virtually RB cord-341342-kyavg4vu 127 22 all all DT cord-341342-kyavg4vu 127 23 of of IN cord-341342-kyavg4vu 127 24 the the DT cord-341342-kyavg4vu 127 25 same same JJ cord-341342-kyavg4vu 127 26 species specie NNS cord-341342-kyavg4vu 127 27 migrated migrate VBN cord-341342-kyavg4vu 127 28 near near IN cord-341342-kyavg4vu 127 29 the the DT cord-341342-kyavg4vu 127 30 dye dye NN cord-341342-kyavg4vu 127 31 front front NN cord-341342-kyavg4vu 127 32 in in IN cord-341342-kyavg4vu 127 33 nondenaturing nondenature VBG cord-341342-kyavg4vu 127 34 PAGE page NN cord-341342-kyavg4vu 127 35 . . . cord-341342-kyavg4vu 128 1 Some some DT cord-341342-kyavg4vu 128 2 minor minor JJ cord-341342-kyavg4vu 128 3 32p 32p CD cord-341342-kyavg4vu 128 4 - - HYPH cord-341342-kyavg4vu 128 5 labeled label VBN cord-341342-kyavg4vu 128 6 bands band NNS cord-341342-kyavg4vu 128 7 were be VBD cord-341342-kyavg4vu 128 8 seen see VBN cord-341342-kyavg4vu 128 9 in in IN cord-341342-kyavg4vu 128 10 the the DT cord-341342-kyavg4vu 128 11 nondenaturing nondenaturing NN cord-341342-kyavg4vu 128 12 gel gel NN cord-341342-kyavg4vu 128 13 at at IN cord-341342-kyavg4vu 128 14 positions position NNS cord-341342-kyavg4vu 128 15 other other JJ cord-341342-kyavg4vu 128 16 than than IN cord-341342-kyavg4vu 128 17 that that DT cord-341342-kyavg4vu 128 18 expected expect VBN cord-341342-kyavg4vu 128 19 for for IN cord-341342-kyavg4vu 128 20 N n CD cord-341342-kyavg4vu 128 21 protein protein NN cord-341342-kyavg4vu 128 22 - - HYPH cord-341342-kyavg4vu 128 23 bound bind VBN cord-341342-kyavg4vu 128 24 material material NN cord-341342-kyavg4vu 128 25 . . . cord-341342-kyavg4vu 129 1 However however RB cord-341342-kyavg4vu 129 2 , , , cord-341342-kyavg4vu 129 3 these these DT cord-341342-kyavg4vu 129 4 could could MD cord-341342-kyavg4vu 129 5 not not RB cord-341342-kyavg4vu 129 6 have have VB cord-341342-kyavg4vu 129 7 been be VBN cord-341342-kyavg4vu 129 8 due due JJ cord-341342-kyavg4vu 129 9 to to IN cord-341342-kyavg4vu 129 10 complex complex JJ cord-341342-kyavg4vu 129 11 formation formation NN cord-341342-kyavg4vu 129 12 with with IN cord-341342-kyavg4vu 129 13 N n CD cord-341342-kyavg4vu 129 14 protein protein NN cord-341342-kyavg4vu 129 15 , , , cord-341342-kyavg4vu 129 16 since since IN cord-341342-kyavg4vu 129 17 the the DT cord-341342-kyavg4vu 129 18 same same JJ cord-341342-kyavg4vu 129 19 bands band NNS cord-341342-kyavg4vu 129 20 were be VBD cord-341342-kyavg4vu 129 21 observed observe VBN cord-341342-kyavg4vu 129 22 when when WRB cord-341342-kyavg4vu 129 23 N N NNP cord-341342-kyavg4vu 129 24 protein protein NN cord-341342-kyavg4vu 129 25 synthesis synthesis NN cord-341342-kyavg4vu 129 26 was be VBD cord-341342-kyavg4vu 129 27 abolished abolish VBN cord-341342-kyavg4vu 129 28 by by IN cord-341342-kyavg4vu 129 29 inclusion inclusion NN cord-341342-kyavg4vu 129 30 of of IN cord-341342-kyavg4vu 129 31 the the DT cord-341342-kyavg4vu 129 32 protein protein NN cord-341342-kyavg4vu 129 33 synthesis synthesis NN cord-341342-kyavg4vu 129 34 inhibitor inhibitor NN cord-341342-kyavg4vu 129 35 cycloheximide cycloheximide NN cord-341342-kyavg4vu 129 36 in in IN cord-341342-kyavg4vu 129 37 translation translation NN cord-341342-kyavg4vu 129 38 reactions reaction NNS cord-341342-kyavg4vu 130 1 ( ( -LRB- cord-341342-kyavg4vu 130 2 Fig Fig NNP cord-341342-kyavg4vu 130 3 . . NNP cord-341342-kyavg4vu 130 4 4 4 CD cord-341342-kyavg4vu 130 5 , , , cord-341342-kyavg4vu 130 6 lane lane NNP cord-341342-kyavg4vu 130 7 i i PRP cord-341342-kyavg4vu 130 8 ' ' '' cord-341342-kyavg4vu 130 9 ) ) -RRB- cord-341342-kyavg4vu 130 10 . . . cord-341342-kyavg4vu 131 1 Thus thus RB cord-341342-kyavg4vu 131 2 , , , cord-341342-kyavg4vu 131 3 the the DT cord-341342-kyavg4vu 131 4 N N NNP cord-341342-kyavg4vu 131 5 - - HYPH cord-341342-kyavg4vu 131 6 RNA RNA NNP cord-341342-kyavg4vu 131 7 complex complex NN cord-341342-kyavg4vu 131 8 observed observe VBD cord-341342-kyavg4vu 131 9 in in IN cord-341342-kyavg4vu 131 10 the the DT cord-341342-kyavg4vu 131 11 nondenaturing nondenature VBG cord-341342-kyavg4vu 131 12 gel gel NN cord-341342-kyavg4vu 131 13 assay assay NN cord-341342-kyavg4vu 131 14 must must MD cord-341342-kyavg4vu 131 15 have have VB cord-341342-kyavg4vu 131 16 been be VBN cord-341342-kyavg4vu 131 17 formed form VBN cord-341342-kyavg4vu 131 18 by by IN cord-341342-kyavg4vu 131 19 the the DT cord-341342-kyavg4vu 131 20 binding binding NN cord-341342-kyavg4vu 131 21 of of IN cord-341342-kyavg4vu 131 22 N n CD cord-341342-kyavg4vu 131 23 to to IN cord-341342-kyavg4vu 131 24 some some DT cord-341342-kyavg4vu 131 25 endogenous endogenous JJ cord-341342-kyavg4vu 131 26 RNA RNA NNP cord-341342-kyavg4vu 131 27 species specie NNS cord-341342-kyavg4vu 131 28 in in IN cord-341342-kyavg4vu 131 29 the the DT cord-341342-kyavg4vu 131 30 reticulocyte reticulocyte NN cord-341342-kyavg4vu 131 31 lysate lysate NN cord-341342-kyavg4vu 131 32 . . . cord-341342-kyavg4vu 132 1 That that IN cord-341342-kyavg4vu 132 2 the the DT cord-341342-kyavg4vu 132 3 observed observed JJ cord-341342-kyavg4vu 132 4 complex complex NN cord-341342-kyavg4vu 132 5 was be VBD cord-341342-kyavg4vu 132 6 not not RB cord-341342-kyavg4vu 132 7 due due JJ cord-341342-kyavg4vu 132 8 to to IN cord-341342-kyavg4vu 132 9 translated translate VBN cord-341342-kyavg4vu 132 10 N n CD cord-341342-kyavg4vu 132 11 protein protein NN cord-341342-kyavg4vu 132 12 binding bind VBG cord-341342-kyavg4vu 132 13 to to IN cord-341342-kyavg4vu 132 14 the the DT cord-341342-kyavg4vu 132 15 leader leader NN cord-341342-kyavg4vu 132 16 portion portion NN cord-341342-kyavg4vu 132 17 of of IN cord-341342-kyavg4vu 132 18 its -PRON- PRP$ cord-341342-kyavg4vu 132 19 own own JJ cord-341342-kyavg4vu 132 20 mRNA mrna NN cord-341342-kyavg4vu 132 21 was be VBD cord-341342-kyavg4vu 132 22 also also RB cord-341342-kyavg4vu 132 23 supported support VBN cord-341342-kyavg4vu 132 24 by by IN cord-341342-kyavg4vu 132 25 the the DT cord-341342-kyavg4vu 132 26 observation observation NN cord-341342-kyavg4vu 132 27 that that IN cord-341342-kyavg4vu 132 28 full full JJ cord-341342-kyavg4vu 132 29 - - HYPH cord-341342-kyavg4vu 132 30 length length NN cord-341342-kyavg4vu 132 31 N n CD cord-341342-kyavg4vu 132 32 protein protein NN cord-341342-kyavg4vu 132 33 translated translate VBN cord-341342-kyavg4vu 132 34 from from IN cord-341342-kyavg4vu 132 35 a a DT cord-341342-kyavg4vu 132 36 construct construct NN cord-341342-kyavg4vu 132 37 in in IN cord-341342-kyavg4vu 132 38 which which WDT cord-341342-kyavg4vu 132 39 the the DT cord-341342-kyavg4vu 132 40 MHV MHV NNP cord-341342-kyavg4vu 132 41 leader leader NN cord-341342-kyavg4vu 132 42 sequence sequence NN cord-341342-kyavg4vu 132 43 was be VBD cord-341342-kyavg4vu 132 44 entirely entirely RB cord-341342-kyavg4vu 132 45 deleted delete VBN cord-341342-kyavg4vu 132 46 was be VBD cord-341342-kyavg4vu 132 47 also also RB cord-341342-kyavg4vu 132 48 able able JJ cord-341342-kyavg4vu 132 49 to to TO cord-341342-kyavg4vu 132 50 migrate migrate VB cord-341342-kyavg4vu 132 51 into into IN cord-341342-kyavg4vu 132 52 nondenaturing nondenature VBG cord-341342-kyavg4vu 132 53 gels gel NNS cord-341342-kyavg4vu 132 54 ( ( -LRB- cord-341342-kyavg4vu 132 55 data datum NNS cord-341342-kyavg4vu 132 56 not not RB cord-341342-kyavg4vu 132 57 shown show VBN cord-341342-kyavg4vu 132 58 ) ) -RRB- cord-341342-kyavg4vu 132 59 . . . cord-341342-kyavg4vu 133 1 The the DT cord-341342-kyavg4vu 133 2 same same JJ cord-341342-kyavg4vu 133 3 was be VBD cord-341342-kyavg4vu 133 4 observed observe VBN cord-341342-kyavg4vu 133 5 for for IN cord-341342-kyavg4vu 133 6 VSV VSV NNP cord-341342-kyavg4vu 133 7 - - HYPH cord-341342-kyavg4vu 133 8 N N NNP cord-341342-kyavg4vu 133 9 : : : cord-341342-kyavg4vu 134 1 MHV MHV NNP cord-341342-kyavg4vu 134 2 - - HYPH cord-341342-kyavg4vu 134 3 N n CD cord-341342-kyavg4vu 134 4 fusion fusion NN cord-341342-kyavg4vu 134 5 constructs construct NNS cord-341342-kyavg4vu 134 6 which which WDT cord-341342-kyavg4vu 134 7 had have VBD cord-341342-kyavg4vu 134 8 the the DT cord-341342-kyavg4vu 134 9 5 5 CD cord-341342-kyavg4vu 134 10 ' ' NN cord-341342-kyavg4vu 134 11 end end NN cord-341342-kyavg4vu 134 12 of of IN cord-341342-kyavg4vu 134 13 a a DT cord-341342-kyavg4vu 134 14 heterologous heterologous NN cord-341342-kyavg4vu 135 1 mRNA mRNA NNP cord-341342-kyavg4vu 135 2 substituted substitute VBN cord-341342-kyavg4vu 135 3 for for IN cord-341342-kyavg4vu 135 4 the the DT cord-341342-kyavg4vu 135 5 MHV MHV NNP cord-341342-kyavg4vu 135 6 leader leader NN cord-341342-kyavg4vu 135 7 ( ( -LRB- cord-341342-kyavg4vu 135 8 see see VB cord-341342-kyavg4vu 135 9 below below RB cord-341342-kyavg4vu 135 10 ) ) -RRB- cord-341342-kyavg4vu 135 11 . . . cord-341342-kyavg4vu 136 1 The the DT cord-341342-kyavg4vu 136 2 complex complex NN cord-341342-kyavg4vu 136 3 reported report VBD cord-341342-kyavg4vu 136 4 to to TO cord-341342-kyavg4vu 136 5 occur occur VB cord-341342-kyavg4vu 136 6 between between IN cord-341342-kyavg4vu 136 7 N n CD cord-341342-kyavg4vu 136 8 protein protein NN cord-341342-kyavg4vu 136 9 and and CC cord-341342-kyavg4vu 136 10 MHV MHV NNP cord-341342-kyavg4vu 136 11 leader leader NN cord-341342-kyavg4vu 136 12 RNA RNA NNP cord-341342-kyavg4vu 137 1 [ [ -LRB- cord-341342-kyavg4vu 137 2 29 29 CD cord-341342-kyavg4vu 137 3 ] ] -RRB- cord-341342-kyavg4vu 137 4 may may MD cord-341342-kyavg4vu 137 5 not not RB cord-341342-kyavg4vu 137 6 have have VB cord-341342-kyavg4vu 137 7 formed form VBN cord-341342-kyavg4vu 137 8 under under IN cord-341342-kyavg4vu 137 9 ( ( -LRB- cord-341342-kyavg4vu 137 10 a'-j a'-j RB cord-341342-kyavg4vu 137 11 ' ' '' cord-341342-kyavg4vu 137 12 ) ) -RRB- cord-341342-kyavg4vu 138 1 the the DT cord-341342-kyavg4vu 138 2 conditions condition NNS cord-341342-kyavg4vu 138 3 of of IN cord-341342-kyavg4vu 138 4 the the DT cord-341342-kyavg4vu 138 5 translation translation NN cord-341342-kyavg4vu 138 6 assay assay NN cord-341342-kyavg4vu 138 7 or or CC cord-341342-kyavg4vu 138 8 may may MD cord-341342-kyavg4vu 138 9 not not RB cord-341342-kyavg4vu 138 10 have have VB cord-341342-kyavg4vu 138 11 been be VBN cord-341342-kyavg4vu 138 12 detectable detectable JJ cord-341342-kyavg4vu 138 13 in in IN cord-341342-kyavg4vu 138 14 the the DT cord-341342-kyavg4vu 138 15 electrophoresis electrophoresis NN cord-341342-kyavg4vu 138 16 system system NN cord-341342-kyavg4vu 138 17 used use VBN cord-341342-kyavg4vu 138 18 here here RB cord-341342-kyavg4vu 138 19 . . . cord-341342-kyavg4vu 139 1 The the DT cord-341342-kyavg4vu 139 2 identity identity NN cord-341342-kyavg4vu 139 3 of of IN cord-341342-kyavg4vu 139 4 the the DT cord-341342-kyavg4vu 139 5 R R NNP cord-341342-kyavg4vu 139 6 N N NNP cord-341342-kyavg4vu 139 7 A a NN cord-341342-kyavg4vu 139 8 b b NN cord-341342-kyavg4vu 139 9 o o NN cord-341342-kyavg4vu 139 10 u u NN cord-341342-kyavg4vu 139 11 n n NN cord-341342-kyavg4vu 139 12 d d NN cord-341342-kyavg4vu 139 13 by by IN cord-341342-kyavg4vu 139 14 N n CD cord-341342-kyavg4vu 139 15 protein protein NN cord-341342-kyavg4vu 139 16 in in IN cord-341342-kyavg4vu 139 17 the the DT cord-341342-kyavg4vu 139 18 reticulocyte reticulocyte NNP cord-341342-kyavg4vu 139 19 lysate lysate NN cord-341342-kyavg4vu 139 20 has have VBZ cord-341342-kyavg4vu 139 21 not not RB cord-341342-kyavg4vu 139 22 yet yet RB cord-341342-kyavg4vu 139 23 been be VBN cord-341342-kyavg4vu 139 24 determined determine VBN cord-341342-kyavg4vu 139 25 . . . cord-341342-kyavg4vu 140 1 N N NNP cord-341342-kyavg4vu 140 2 protein protein NN cord-341342-kyavg4vu 140 3 formed form VBD cord-341342-kyavg4vu 140 4 a a DT cord-341342-kyavg4vu 140 5 discrete discrete JJ cord-341342-kyavg4vu 140 6 band band NN cord-341342-kyavg4vu 140 7 rather rather RB cord-341342-kyavg4vu 140 8 than than IN cord-341342-kyavg4vu 140 9 a a DT cord-341342-kyavg4vu 140 10 heterogeneous heterogeneous JJ cord-341342-kyavg4vu 140 11 smear smear NN cord-341342-kyavg4vu 140 12 in in IN cord-341342-kyavg4vu 140 13 nondenaturing nondenature VBG cord-341342-kyavg4vu 140 14 P p NN cord-341342-kyavg4vu 140 15 A a NN cord-341342-kyavg4vu 140 16 G g NN cord-341342-kyavg4vu 140 17 E e NN cord-341342-kyavg4vu 140 18 , , , cord-341342-kyavg4vu 140 19 suggesting suggest VBG cord-341342-kyavg4vu 140 20 that that IN cord-341342-kyavg4vu 140 21 it -PRON- PRP cord-341342-kyavg4vu 140 22 was be VBD cord-341342-kyavg4vu 140 23 binding bind VBG cord-341342-kyavg4vu 140 24 150 150 CD cord-341342-kyavg4vu 140 25 P.S. P.S. NNP cord-341342-kyavg4vu 140 26 Masters Masters NNPS cord-341342-kyavg4vu 140 27 to to IN cord-341342-kyavg4vu 140 28 a a DT cord-341342-kyavg4vu 140 29 particular particular JJ cord-341342-kyavg4vu 140 30 RNA RNA NNP cord-341342-kyavg4vu 140 31 species specie NNS cord-341342-kyavg4vu 140 32 or or CC cord-341342-kyavg4vu 140 33 at at IN cord-341342-kyavg4vu 140 34 least least JJS cord-341342-kyavg4vu 140 35 to to IN cord-341342-kyavg4vu 140 36 a a DT cord-341342-kyavg4vu 140 37 set set NN cord-341342-kyavg4vu 140 38 of of IN cord-341342-kyavg4vu 140 39 species specie NNS cord-341342-kyavg4vu 140 40 of of IN cord-341342-kyavg4vu 140 41 fairly fairly RB cord-341342-kyavg4vu 140 42 uniform uniform JJ cord-341342-kyavg4vu 140 43 size size NN cord-341342-kyavg4vu 140 44 . . . cord-341342-kyavg4vu 141 1 Moreover moreover RB cord-341342-kyavg4vu 141 2 , , , cord-341342-kyavg4vu 141 3 a a DT cord-341342-kyavg4vu 141 4 band band NN cord-341342-kyavg4vu 141 5 of of IN cord-341342-kyavg4vu 141 6 identical identical JJ cord-341342-kyavg4vu 141 7 mobility mobility NN cord-341342-kyavg4vu 141 8 was be VBD cord-341342-kyavg4vu 141 9 formed form VBN cord-341342-kyavg4vu 141 10 by by IN cord-341342-kyavg4vu 141 11 N n CD cord-341342-kyavg4vu 141 12 protein protein NN cord-341342-kyavg4vu 141 13 that that WDT cord-341342-kyavg4vu 141 14 had have VBD cord-341342-kyavg4vu 141 15 been be VBN cord-341342-kyavg4vu 141 16 translated translate VBN cord-341342-kyavg4vu 141 17 in in IN cord-341342-kyavg4vu 141 18 a a DT cord-341342-kyavg4vu 141 19 wheat wheat NN cord-341342-kyavg4vu 141 20 germ germ NN cord-341342-kyavg4vu 141 21 extract extract NN cord-341342-kyavg4vu 141 22 ( ( -LRB- cord-341342-kyavg4vu 141 23 data datum NNS cord-341342-kyavg4vu 141 24 not not RB cord-341342-kyavg4vu 141 25 shown show VBN cord-341342-kyavg4vu 141 26 ) ) -RRB- cord-341342-kyavg4vu 141 27 , , , cord-341342-kyavg4vu 141 28 indicating indicate VBG cord-341342-kyavg4vu 141 29 that that IN cord-341342-kyavg4vu 141 30 the the DT cord-341342-kyavg4vu 141 31 unknown unknown JJ cord-341342-kyavg4vu 141 32 RNA RNA NNP cord-341342-kyavg4vu 141 33 species specie NNS cord-341342-kyavg4vu 141 34 was be VBD cord-341342-kyavg4vu 141 35 not not RB cord-341342-kyavg4vu 141 36 unique unique JJ cord-341342-kyavg4vu 141 37 to to IN cord-341342-kyavg4vu 141 38 reticulocytes reticulocyte NNS cord-341342-kyavg4vu 141 39 . . . cord-341342-kyavg4vu 142 1 This this DT cord-341342-kyavg4vu 142 2 non non JJ cord-341342-kyavg4vu 142 3 - - JJ cord-341342-kyavg4vu 142 4 sequence sequence JJ cord-341342-kyavg4vu 142 5 - - HYPH cord-341342-kyavg4vu 142 6 specific specific JJ cord-341342-kyavg4vu 142 7 mode mode NN cord-341342-kyavg4vu 142 8 of of IN cord-341342-kyavg4vu 142 9 RNA RNA NNP cord-341342-kyavg4vu 142 10 binding binding NN cord-341342-kyavg4vu 142 11 was be VBD cord-341342-kyavg4vu 142 12 not not RB cord-341342-kyavg4vu 142 13 unexpected unexpected JJ cord-341342-kyavg4vu 142 14 . . . cord-341342-kyavg4vu 143 1 The the DT cord-341342-kyavg4vu 143 2 N n CD cord-341342-kyavg4vu 143 3 protein protein NN cord-341342-kyavg4vu 143 4 of of IN cord-341342-kyavg4vu 143 5 MHV MHV NNP cord-341342-kyavg4vu 143 6 has have VBZ cord-341342-kyavg4vu 143 7 been be VBN cord-341342-kyavg4vu 143 8 shown show VBN cord-341342-kyavg4vu 143 9 previously previously RB cord-341342-kyavg4vu 143 10 to to TO cord-341342-kyavg4vu 143 11 bind bind VB cord-341342-kyavg4vu 143 12 in in IN cord-341342-kyavg4vu 143 13 vitro vitro FW cord-341342-kyavg4vu 143 14 to to IN cord-341342-kyavg4vu 143 15 RNA RNA NNP cord-341342-kyavg4vu 143 16 of of IN cord-341342-kyavg4vu 143 17 nonviral nonviral JJ cord-341342-kyavg4vu 143 18 origin origin NN cord-341342-kyavg4vu 143 19 by by IN cord-341342-kyavg4vu 143 20 a a DT cord-341342-kyavg4vu 143 21 labeled label VBN cord-341342-kyavg4vu 143 22 RNA RNA NNP cord-341342-kyavg4vu 143 23 overlay overlay NN cord-341342-kyavg4vu 143 24 protein protein NN cord-341342-kyavg4vu 143 25 blot blot NN cord-341342-kyavg4vu 143 26 assay assay NN cord-341342-kyavg4vu 143 27 [ [ -LRB- cord-341342-kyavg4vu 143 28 22 22 CD cord-341342-kyavg4vu 143 29 , , , cord-341342-kyavg4vu 143 30 29 29 CD cord-341342-kyavg4vu 143 31 ] ] -RRB- cord-341342-kyavg4vu 143 32 , , , cord-341342-kyavg4vu 143 33 and and CC cord-341342-kyavg4vu 143 34 , , , cord-341342-kyavg4vu 143 35 indeed indeed RB cord-341342-kyavg4vu 143 36 such such JJ cord-341342-kyavg4vu 143 37 non non JJ cord-341342-kyavg4vu 143 38 - - JJ cord-341342-kyavg4vu 143 39 sequence sequence JJ cord-341342-kyavg4vu 143 40 - - HYPH cord-341342-kyavg4vu 143 41 specific specific JJ cord-341342-kyavg4vu 143 42 binding binding NN cord-341342-kyavg4vu 143 43 must must MD cord-341342-kyavg4vu 143 44 be be VB cord-341342-kyavg4vu 143 45 the the DT cord-341342-kyavg4vu 143 46 principal principal JJ cord-341342-kyavg4vu 143 47 type type NN cord-341342-kyavg4vu 143 48 of of IN cord-341342-kyavg4vu 143 49 protein protein NN cord-341342-kyavg4vu 143 50 - - HYPH cord-341342-kyavg4vu 143 51 RNA rna NN cord-341342-kyavg4vu 143 52 contact contact NN cord-341342-kyavg4vu 143 53 along along IN cord-341342-kyavg4vu 143 54 the the DT cord-341342-kyavg4vu 143 55 extensive extensive JJ cord-341342-kyavg4vu 143 56 length length NN cord-341342-kyavg4vu 143 57 of of IN cord-341342-kyavg4vu 143 58 the the DT cord-341342-kyavg4vu 143 59 coronavirus coronavirus NN cord-341342-kyavg4vu 143 60 nucleocapsid nucleocapsid NN cord-341342-kyavg4vu 143 61 . . . cord-341342-kyavg4vu 144 1 The the DT cord-341342-kyavg4vu 144 2 ability ability NN cord-341342-kyavg4vu 144 3 of of IN cord-341342-kyavg4vu 144 4 translated translate VBN cord-341342-kyavg4vu 144 5 N n CD cord-341342-kyavg4vu 144 6 protein protein NN cord-341342-kyavg4vu 144 7 to to TO cord-341342-kyavg4vu 144 8 migrate migrate VB cord-341342-kyavg4vu 144 9 into into IN cord-341342-kyavg4vu 144 10 a a DT cord-341342-kyavg4vu 144 11 nondenaturing nondenature VBG cord-341342-kyavg4vu 144 12 gel gel NN cord-341342-kyavg4vu 144 13 was be VBD cord-341342-kyavg4vu 144 14 next next RB cord-341342-kyavg4vu 144 15 used use VBN cord-341342-kyavg4vu 144 16 as as IN cord-341342-kyavg4vu 144 17 an an DT cord-341342-kyavg4vu 144 18 assay assay NN cord-341342-kyavg4vu 144 19 to to TO cord-341342-kyavg4vu 144 20 map map VB cord-341342-kyavg4vu 144 21 the the DT cord-341342-kyavg4vu 144 22 RNA rna NN cord-341342-kyavg4vu 144 23 - - HYPH cord-341342-kyavg4vu 144 24 binding bind VBG cord-341342-kyavg4vu 144 25 function function NN cord-341342-kyavg4vu 144 26 of of IN cord-341342-kyavg4vu 144 27 the the DT cord-341342-kyavg4vu 144 28 N N NNP cord-341342-kyavg4vu 144 29 protein protein NN cord-341342-kyavg4vu 144 30 molecule molecule NN cord-341342-kyavg4vu 144 31 . . . cord-341342-kyavg4vu 145 1 A a DT cord-341342-kyavg4vu 145 2 set set NN cord-341342-kyavg4vu 145 3 of of IN cord-341342-kyavg4vu 145 4 N n CD cord-341342-kyavg4vu 145 5 protein protein NN cord-341342-kyavg4vu 145 6 deletion deletion NN cord-341342-kyavg4vu 145 7 mutants mutant NNS cord-341342-kyavg4vu 145 8 with with IN cord-341342-kyavg4vu 145 9 successively successively RB cord-341342-kyavg4vu 145 10 larger large JJR cord-341342-kyavg4vu 145 11 carboxyterminal carboxyterminal JJ cord-341342-kyavg4vu 145 12 truncations truncation NNS cord-341342-kyavg4vu 145 13 ( ( -LRB- cord-341342-kyavg4vu 145 14 designated designate VBN cord-341342-kyavg4vu 145 15 N N NNP cord-341342-kyavg4vu 145 16 / / SYM cord-341342-kyavg4vu 145 17 Ace Ace NNP cord-341342-kyavg4vu 145 18 , , , cord-341342-kyavg4vu 145 19 N N NNP cord-341342-kyavg4vu 145 20 / / SYM cord-341342-kyavg4vu 145 21 Bsm Bsm NNP cord-341342-kyavg4vu 145 22 , , , cord-341342-kyavg4vu 145 23 N N NNP cord-341342-kyavg4vu 145 24 / / SYM cord-341342-kyavg4vu 145 25 Sea Sea NNP cord-341342-kyavg4vu 145 26 , , , cord-341342-kyavg4vu 145 27 N N NNP cord-341342-kyavg4vu 145 28 / / SYM cord-341342-kyavg4vu 145 29 Eco Eco NNP cord-341342-kyavg4vu 145 30 and and CC cord-341342-kyavg4vu 145 31 N N NNP cord-341342-kyavg4vu 145 32 / / SYM cord-341342-kyavg4vu 145 33 Spe Spe NNP cord-341342-kyavg4vu 145 34 ) ) -RRB- cord-341342-kyavg4vu 145 35 was be VBD cord-341342-kyavg4vu 145 36 generated generate VBN cord-341342-kyavg4vu 145 37 by by IN cord-341342-kyavg4vu 145 38 translation translation NN cord-341342-kyavg4vu 145 39 of of IN cord-341342-kyavg4vu 145 40 run run VB cord-341342-kyavg4vu 145 41 - - HYPH cord-341342-kyavg4vu 145 42 off off RP cord-341342-kyavg4vu 145 43 transcripts transcript NNS cord-341342-kyavg4vu 145 44 synthesized synthesize VBN cord-341342-kyavg4vu 145 45 from from IN cord-341342-kyavg4vu 145 46 transcription transcription NN cord-341342-kyavg4vu 145 47 vectors vector NNS cord-341342-kyavg4vu 145 48 that that WDT cord-341342-kyavg4vu 145 49 had have VBD cord-341342-kyavg4vu 145 50 been be VBN cord-341342-kyavg4vu 145 51 linearized linearize VBN cord-341342-kyavg4vu 145 52 with with IN cord-341342-kyavg4vu 145 53 the the DT cord-341342-kyavg4vu 145 54 restriction restriction NN cord-341342-kyavg4vu 145 55 enzymes enzyme NNS cord-341342-kyavg4vu 145 56 AccI AccI NNP cord-341342-kyavg4vu 145 57 , , , cord-341342-kyavg4vu 145 58 BsmI BsmI NNP cord-341342-kyavg4vu 145 59 , , , cord-341342-kyavg4vu 145 60 ScaI ScaI NNP cord-341342-kyavg4vu 145 61 , , , cord-341342-kyavg4vu 145 62 EcoRI ecori NN cord-341342-kyavg4vu 145 63 , , , cord-341342-kyavg4vu 145 64 or or CC cord-341342-kyavg4vu 145 65 SpeI SpeI NNP cord-341342-kyavg4vu 145 66 ( ( -LRB- cord-341342-kyavg4vu 145 67 Fig Fig NNP cord-341342-kyavg4vu 145 68 . . . cord-341342-kyavg4vu 145 69 1 1 CD cord-341342-kyavg4vu 145 70 ) ) -RRB- cord-341342-kyavg4vu 145 71 . . . cord-341342-kyavg4vu 146 1 These these DT cord-341342-kyavg4vu 146 2 variants variant NNS cord-341342-kyavg4vu 146 3 contained contain VBN cord-341342-kyavg4vu 146 4 , , , cord-341342-kyavg4vu 146 5 respectively respectively RB cord-341342-kyavg4vu 146 6 , , , cord-341342-kyavg4vu 146 7 deletions deletion NNS cord-341342-kyavg4vu 146 8 of of IN cord-341342-kyavg4vu 146 9 57 57 CD cord-341342-kyavg4vu 146 10 , , , cord-341342-kyavg4vu 146 11 77 77 CD cord-341342-kyavg4vu 146 12 , , , cord-341342-kyavg4vu 146 13 94 94 CD cord-341342-kyavg4vu 146 14 , , , cord-341342-kyavg4vu 146 15 118 118 CD cord-341342-kyavg4vu 146 16 , , , cord-341342-kyavg4vu 146 17 and and CC cord-341342-kyavg4vu 146 18 148 148 CD cord-341342-kyavg4vu 146 19 amino amino JJ cord-341342-kyavg4vu 146 20 acids acid NNS cord-341342-kyavg4vu 146 21 . . . cord-341342-kyavg4vu 147 1 In in IN cord-341342-kyavg4vu 147 2 addition addition NN cord-341342-kyavg4vu 147 3 , , , cord-341342-kyavg4vu 147 4 the the DT cord-341342-kyavg4vu 147 5 internal internal JJ cord-341342-kyavg4vu 147 6 deletion deletion NN cord-341342-kyavg4vu 147 7 contained contain VBN cord-341342-kyavg4vu 147 8 in in IN cord-341342-kyavg4vu 147 9 the the DT cord-341342-kyavg4vu 147 10 N n CD cord-341342-kyavg4vu 147 11 gene gene NN cord-341342-kyavg4vu 147 12 of of IN cord-341342-kyavg4vu 147 13 a a DT cord-341342-kyavg4vu 147 14 temperature temperature NN cord-341342-kyavg4vu 147 15 - - HYPH cord-341342-kyavg4vu 147 16 sensitive sensitive JJ cord-341342-kyavg4vu 147 17 and and CC cord-341342-kyavg4vu 147 18 thermolabile thermolabile JJ cord-341342-kyavg4vu 147 19 MHV MHV NNP cord-341342-kyavg4vu 147 20 - - HYPH cord-341342-kyavg4vu 147 21 A59 A59 NNP cord-341342-kyavg4vu 147 22 mutant mutant NN cord-341342-kyavg4vu 147 23 , , , cord-341342-kyavg4vu 147 24 Albany-4 Albany-4 NNP cord-341342-kyavg4vu 147 25 , , , cord-341342-kyavg4vu 147 26 was be VBD cord-341342-kyavg4vu 147 27 transferred transfer VBN cord-341342-kyavg4vu 147 28 to to IN cord-341342-kyavg4vu 147 29 a a DT cord-341342-kyavg4vu 147 30 transcription transcription NN cord-341342-kyavg4vu 147 31 vector vector NN cord-341342-kyavg4vu 147 32 . . . cord-341342-kyavg4vu 148 1 The the DT cord-341342-kyavg4vu 148 2 mutant mutant JJ cord-341342-kyavg4vu 148 3 N N NNP cord-341342-kyavg4vu 148 4 protein protein NN cord-341342-kyavg4vu 148 5 translated translate VBN cord-341342-kyavg4vu 148 6 from from IN cord-341342-kyavg4vu 148 7 this this DT cord-341342-kyavg4vu 148 8 construct construct NN cord-341342-kyavg4vu 148 9 ( ( -LRB- cord-341342-kyavg4vu 148 10 N N NNP cord-341342-kyavg4vu 148 11 / / SYM cord-341342-kyavg4vu 148 12 Alb4 Alb4 NNP cord-341342-kyavg4vu 148 13 ) ) -RRB- cord-341342-kyavg4vu 148 14 contained contain VBD cord-341342-kyavg4vu 148 15 an an DT cord-341342-kyavg4vu 148 16 in in IN cord-341342-kyavg4vu 148 17 - - HYPH cord-341342-kyavg4vu 148 18 phase phase NN cord-341342-kyavg4vu 148 19 deletion deletion NN cord-341342-kyavg4vu 148 20 of of IN cord-341342-kyavg4vu 148 21 amino amino JJ cord-341342-kyavg4vu 148 22 acids acid NNS cord-341342-kyavg4vu 148 23 380 380 CD cord-341342-kyavg4vu 148 24 through through IN cord-341342-kyavg4vu 148 25 408 408 CD cord-341342-kyavg4vu 148 26 but but CC cord-341342-kyavg4vu 148 27 was be VBD cord-341342-kyavg4vu 148 28 everywhere everywhere RB cord-341342-kyavg4vu 148 29 else else RB cord-341342-kyavg4vu 148 30 identical identical JJ cord-341342-kyavg4vu 148 31 to to IN cord-341342-kyavg4vu 148 32 wild wild JJ cord-341342-kyavg4vu 148 33 type type NN cord-341342-kyavg4vu 148 34 N n NN cord-341342-kyavg4vu 148 35 ( ( -LRB- cord-341342-kyavg4vu 148 36 [ [ -LRB- cord-341342-kyavg4vu 148 37 17 17 CD cord-341342-kyavg4vu 148 38 ] ] -RRB- cord-341342-kyavg4vu 148 39 ; ; : cord-341342-kyavg4vu 149 1 Koetzner Koetzner NNP cord-341342-kyavg4vu 149 2 et et NNP cord-341342-kyavg4vu 149 3 al al NNP cord-341342-kyavg4vu 149 4 . . . cord-341342-kyavg4vu 150 1 , , , cord-341342-kyavg4vu 150 2 manuscript manuscript NN cord-341342-kyavg4vu 150 3 in in IN cord-341342-kyavg4vu 150 4 prep prep NN cord-341342-kyavg4vu 150 5 . . . cord-341342-kyavg4vu 150 6 ) ) -RRB- cord-341342-kyavg4vu 150 7 . . . cord-341342-kyavg4vu 151 1 These these DT cord-341342-kyavg4vu 151 2 six six CD cord-341342-kyavg4vu 151 3 mutants mutant NNS cord-341342-kyavg4vu 151 4 , , , cord-341342-kyavg4vu 151 5 as as RB cord-341342-kyavg4vu 151 6 well well RB cord-341342-kyavg4vu 151 7 as as IN cord-341342-kyavg4vu 151 8 full full JJ cord-341342-kyavg4vu 151 9 - - HYPH cord-341342-kyavg4vu 151 10 length length NN cord-341342-kyavg4vu 151 11 N n CD cord-341342-kyavg4vu 151 12 protein protein NN cord-341342-kyavg4vu 151 13 , , , cord-341342-kyavg4vu 151 14 were be VBD cord-341342-kyavg4vu 151 15 analyzed analyze VBN cord-341342-kyavg4vu 151 16 by by IN cord-341342-kyavg4vu 151 17 SDS SDS NNP cord-341342-kyavg4vu 151 18 - - HYPH cord-341342-kyavg4vu 151 19 PAGE PAGE NNP cord-341342-kyavg4vu 151 20 and and CC cord-341342-kyavg4vu 151 21 by by IN cord-341342-kyavg4vu 151 22 nondenaturing nondenature VBG cord-341342-kyavg4vu 151 23 PAGE PAGE NNP cord-341342-kyavg4vu 151 24 ( ( -LRB- cord-341342-kyavg4vu 151 25 Fig Fig NNP cord-341342-kyavg4vu 151 26 . . . cord-341342-kyavg4vu 151 27 5 5 CD cord-341342-kyavg4vu 151 28 ) ) -RRB- cord-341342-kyavg4vu 151 29 . . . cord-341342-kyavg4vu 152 1 On on IN cord-341342-kyavg4vu 152 2 SDS SDS NNP cord-341342-kyavg4vu 152 3 - - HYPH cord-341342-kyavg4vu 152 4 PAGE PAGE NNP cord-341342-kyavg4vu 152 5 , , , cord-341342-kyavg4vu 152 6 all all DT cord-341342-kyavg4vu 152 7 migrated migrate VBN cord-341342-kyavg4vu 152 8 as as IN cord-341342-kyavg4vu 152 9 discrete discrete JJ cord-341342-kyavg4vu 152 10 bands band NNS cord-341342-kyavg4vu 152 11 of of IN cord-341342-kyavg4vu 152 12 the the DT cord-341342-kyavg4vu 152 13 expected expect VBN cord-341342-kyavg4vu 152 14 relative relative JJ cord-341342-kyavg4vu 152 15 mobilities mobility NNS cord-341342-kyavg4vu 152 16 and and CC cord-341342-kyavg4vu 152 17 were be VBD cord-341342-kyavg4vu 152 18 largely largely RB cord-341342-kyavg4vu 152 19 unaffected unaffected JJ cord-341342-kyavg4vu 152 20 by by IN cord-341342-kyavg4vu 152 21 treatment treatment NN cord-341342-kyavg4vu 152 22 with with IN cord-341342-kyavg4vu 152 23 RNase RNase NNP cord-341342-kyavg4vu 152 24 A. a. NN cord-341342-kyavg4vu 153 1 The the DT cord-341342-kyavg4vu 153 2 N N NNP cord-341342-kyavg4vu 153 3 / / SYM cord-341342-kyavg4vu 153 4 Ace Ace NNP cord-341342-kyavg4vu 153 5 mutant mutant NN cord-341342-kyavg4vu 153 6 showed show VBD cord-341342-kyavg4vu 153 7 some some DT cord-341342-kyavg4vu 153 8 degree degree NN cord-341342-kyavg4vu 153 9 of of IN cord-341342-kyavg4vu 153 10 heterogeneity heterogeneity NN cord-341342-kyavg4vu 153 11 in in IN cord-341342-kyavg4vu 153 12 both both CC cord-341342-kyavg4vu 153 13 the the DT cord-341342-kyavg4vu 153 14 presence presence NN cord-341342-kyavg4vu 153 15 and and CC cord-341342-kyavg4vu 153 16 absence absence NN cord-341342-kyavg4vu 153 17 of of IN cord-341342-kyavg4vu 153 18 RNase RNase NNP cord-341342-kyavg4vu 153 19 A A NNP cord-341342-kyavg4vu 153 20 ( ( -LRB- cord-341342-kyavg4vu 153 21 Fig Fig NNP cord-341342-kyavg4vu 153 22 . . NNP cord-341342-kyavg4vu 153 23 5 5 CD cord-341342-kyavg4vu 153 24 , , , cord-341342-kyavg4vu 153 25 lanes lane NNS cord-341342-kyavg4vu 153 26 e e NN cord-341342-kyavg4vu 153 27 and and CC cord-341342-kyavg4vu 153 28 f f NNP cord-341342-kyavg4vu 153 29 ) ) -RRB- cord-341342-kyavg4vu 153 30 . . . cord-341342-kyavg4vu 154 1 Since since IN cord-341342-kyavg4vu 154 2 this this DT cord-341342-kyavg4vu 154 3 protein protein NN cord-341342-kyavg4vu 154 4 product product NN cord-341342-kyavg4vu 154 5 terminated terminate VBN cord-341342-kyavg4vu 154 6 in in IN cord-341342-kyavg4vu 154 7 the the DT cord-341342-kyavg4vu 154 8 basic basic JJ cord-341342-kyavg4vu 154 9 amino amino NN cord-341342-kyavg4vu 154 10 acid acid NN cord-341342-kyavg4vu 154 11 - - HYPH cord-341342-kyavg4vu 154 12 rich rich JJ cord-341342-kyavg4vu 154 13 sequence sequence NN cord-341342-kyavg4vu 154 14 KPQRKGR KPQRKGR NNP cord-341342-kyavg4vu 154 15 , , , cord-341342-kyavg4vu 154 16 it -PRON- PRP cord-341342-kyavg4vu 154 17 possibly possibly RB cord-341342-kyavg4vu 154 18 was be VBD cord-341342-kyavg4vu 154 19 rendered render VBN cord-341342-kyavg4vu 154 20 sensitive sensitive JJ cord-341342-kyavg4vu 154 21 to to IN cord-341342-kyavg4vu 154 22 degradation degradation NN cord-341342-kyavg4vu 154 23 by by IN cord-341342-kyavg4vu 154 24 trypsin trypsin NN cord-341342-kyavg4vu 154 25 - - HYPH cord-341342-kyavg4vu 154 26 like like JJ cord-341342-kyavg4vu 154 27 proteolytic proteolytic JJ cord-341342-kyavg4vu 154 28 activities activity NNS cord-341342-kyavg4vu 154 29 within within IN cord-341342-kyavg4vu 154 30 the the DT cord-341342-kyavg4vu 154 31 reticulocyte reticulocyte NNP cord-341342-kyavg4vu 154 32 lysate lysate NN cord-341342-kyavg4vu 154 33 . . . cord-341342-kyavg4vu 155 1 When when WRB cord-341342-kyavg4vu 155 2 analyzed analyze VBN cord-341342-kyavg4vu 155 3 for for IN cord-341342-kyavg4vu 155 4 the the DT cord-341342-kyavg4vu 155 5 ability ability NN cord-341342-kyavg4vu 155 6 to to TO cord-341342-kyavg4vu 155 7 bind bind VB cord-341342-kyavg4vu 155 8 to to IN cord-341342-kyavg4vu 155 9 RNA RNA NNP cord-341342-kyavg4vu 155 10 , , , cord-341342-kyavg4vu 155 11 only only RB cord-341342-kyavg4vu 155 12 two two CD cord-341342-kyavg4vu 155 13 of of IN cord-341342-kyavg4vu 155 14 the the DT cord-341342-kyavg4vu 155 15 N n CD cord-341342-kyavg4vu 155 16 mutants mutant NNS cord-341342-kyavg4vu 155 17 , , , cord-341342-kyavg4vu 155 18 N N NNP cord-341342-kyavg4vu 155 19 / / SYM cord-341342-kyavg4vu 155 20 Acc Acc NNP cord-341342-kyavg4vu 155 21 and and CC cord-341342-kyavg4vu 155 22 N N NNP cord-341342-kyavg4vu 155 23 / / SYM cord-341342-kyavg4vu 155 24 Alb4 Alb4 NNP cord-341342-kyavg4vu 155 25 , , , cord-341342-kyavg4vu 155 26 were be VBD cord-341342-kyavg4vu 155 27 able able JJ cord-341342-kyavg4vu 155 28 to to TO cord-341342-kyavg4vu 155 29 enter enter VB cord-341342-kyavg4vu 155 30 a a DT cord-341342-kyavg4vu 155 31 nondenaturing nondenaturing NN cord-341342-kyavg4vu 155 32 get get NN cord-341342-kyavg4vu 155 33 to to IN cord-341342-kyavg4vu 155 34 a a DT cord-341342-kyavg4vu 155 35 significant significant JJ cord-341342-kyavg4vu 155 36 quantitative quantitative JJ cord-341342-kyavg4vu 155 37 extent extent NN cord-341342-kyavg4vu 155 38 by by IN cord-341342-kyavg4vu 155 39 comparison comparison NN cord-341342-kyavg4vu 155 40 with with IN cord-341342-kyavg4vu 155 41 full full JJ cord-341342-kyavg4vu 155 42 - - HYPH cord-341342-kyavg4vu 155 43 length length NN cord-341342-kyavg4vu 155 44 N n CD cord-341342-kyavg4vu 155 45 protein protein NN cord-341342-kyavg4vu 155 46 ( ( -LRB- cord-341342-kyavg4vu 155 47 Fig Fig NNP cord-341342-kyavg4vu 155 48 . . NNP cord-341342-kyavg4vu 155 49 5 5 CD cord-341342-kyavg4vu 155 50 , , , cord-341342-kyavg4vu 155 51 lanes lane VBZ cord-341342-kyavg4vu 155 52 a a DT cord-341342-kyavg4vu 155 53 ' ' '' cord-341342-kyavg4vu 155 54 to to IN cord-341342-kyavg4vu 155 55 p p NN cord-341342-kyavg4vu 155 56 ' ' '' cord-341342-kyavg4vu 155 57 ) ) -RRB- cord-341342-kyavg4vu 155 58 . . . cord-341342-kyavg4vu 156 1 This this DT cord-341342-kyavg4vu 156 2 migration migration NN cord-341342-kyavg4vu 156 3 was be VBD cord-341342-kyavg4vu 156 4 completely completely RB cord-341342-kyavg4vu 156 5 inhibited inhibit VBN cord-341342-kyavg4vu 156 6 by by IN cord-341342-kyavg4vu 156 7 prior prior JJ cord-341342-kyavg4vu 156 8 incubation incubation NN cord-341342-kyavg4vu 156 9 with with IN cord-341342-kyavg4vu 156 10 RNase RNase NNP cord-341342-kyavg4vu 156 11 A. a. NN cord-341342-kyavg4vu 157 1 The the DT cord-341342-kyavg4vu 157 2 remainder remainder NN cord-341342-kyavg4vu 157 3 of of IN cord-341342-kyavg4vu 157 4 the the DT cord-341342-kyavg4vu 157 5 carboxyterminal carboxyterminal NNP cord-341342-kyavg4vu 157 6 truncated truncated NNP cord-341342-kyavg4vu 157 7 N n CD cord-341342-kyavg4vu 157 8 proteins protein NNS cord-341342-kyavg4vu 157 9 were be VBD cord-341342-kyavg4vu 157 10 only only RB cord-341342-kyavg4vu 157 11 minimally minimally RB cord-341342-kyavg4vu 157 12 detectable detectable JJ cord-341342-kyavg4vu 157 13 or or CC cord-341342-kyavg4vu 157 14 were be VBD cord-341342-kyavg4vu 157 15 undetectable undetectable JJ cord-341342-kyavg4vu 157 16 by by IN cord-341342-kyavg4vu 157 17 nondenaturing nondenature VBG cord-341342-kyavg4vu 157 18 PAGE page NN cord-341342-kyavg4vu 157 19 . . . cord-341342-kyavg4vu 158 1 An an DT cord-341342-kyavg4vu 158 2 estimate estimate NN cord-341342-kyavg4vu 158 3 of of IN cord-341342-kyavg4vu 158 4 the the DT cord-341342-kyavg4vu 158 5 relative relative JJ cord-341342-kyavg4vu 158 6 RNA rna NN cord-341342-kyavg4vu 158 7 - - HYPH cord-341342-kyavg4vu 158 8 binding bind VBG cord-341342-kyavg4vu 158 9 ability ability NN cord-341342-kyavg4vu 158 10 of of IN cord-341342-kyavg4vu 158 11 each each DT cord-341342-kyavg4vu 158 12 N N NNP cord-341342-kyavg4vu 158 13 protein protein NN cord-341342-kyavg4vu 158 14 construct construct NN cord-341342-kyavg4vu 158 15 was be VBD cord-341342-kyavg4vu 158 16 made make VBN cord-341342-kyavg4vu 158 17 by by IN cord-341342-kyavg4vu 158 18 determining determine VBG cord-341342-kyavg4vu 158 19 the the DT cord-341342-kyavg4vu 158 20 ratio ratio NN cord-341342-kyavg4vu 158 21 of of IN cord-341342-kyavg4vu 158 22 cpm cpm NNP cord-341342-kyavg4vu 158 23 of of IN cord-341342-kyavg4vu 158 24 translated translate VBN cord-341342-kyavg4vu 158 25 protein protein NN cord-341342-kyavg4vu 158 26 that that WDT cord-341342-kyavg4vu 158 27 had have VBD cord-341342-kyavg4vu 158 28 banded band VBN cord-341342-kyavg4vu 158 29 in in IN cord-341342-kyavg4vu 158 30 a a DT cord-341342-kyavg4vu 158 31 nondenaturing nondenature VBG cord-341342-kyavg4vu 158 32 gel gel NN cord-341342-kyavg4vu 158 33 compared compare VBN cord-341342-kyavg4vu 158 34 to to IN cord-341342-kyavg4vu 158 35 the the DT cord-341342-kyavg4vu 158 36 cpm cpm NN cord-341342-kyavg4vu 158 37 of of IN cord-341342-kyavg4vu 158 38 an an DT cord-341342-kyavg4vu 158 39 identical identical JJ cord-341342-kyavg4vu 158 40 sample sample NN cord-341342-kyavg4vu 158 41 that that WDT cord-341342-kyavg4vu 158 42 had have VBD cord-341342-kyavg4vu 158 43 banded band VBN cord-341342-kyavg4vu 158 44 in in IN cord-341342-kyavg4vu 158 45 SDS SDS NNP cord-341342-kyavg4vu 158 46 - - HYPH cord-341342-kyavg4vu 158 47 PAGE PAGE NNP cord-341342-kyavg4vu 158 48 ( ( -LRB- cord-341342-kyavg4vu 158 49 Table table NN cord-341342-kyavg4vu 158 50 1 1 CD cord-341342-kyavg4vu 158 51 ) ) -RRB- cord-341342-kyavg4vu 158 52 . . . cord-341342-kyavg4vu 159 1 By by IN cord-341342-kyavg4vu 159 2 this this DT cord-341342-kyavg4vu 159 3 analysis analysis NN cord-341342-kyavg4vu 159 4 , , , cord-341342-kyavg4vu 159 5 the the DT cord-341342-kyavg4vu 159 6 N N NNP cord-341342-kyavg4vu 159 7 / / SYM cord-341342-kyavg4vu 159 8 Ace Ace NNP cord-341342-kyavg4vu 159 9 construct construct NN cord-341342-kyavg4vu 159 10 retained retain VBN cord-341342-kyavg4vu 159 11 the the DT cord-341342-kyavg4vu 159 12 major major JJ cord-341342-kyavg4vu 159 13 part part NN cord-341342-kyavg4vu 159 14 of of IN cord-341342-kyavg4vu 159 15 the the DT cord-341342-kyavg4vu 159 16 RNA RNA NNP cord-341342-kyavg4vu 159 17 - - HYPH cord-341342-kyavg4vu 159 18 binding bind VBG cord-341342-kyavg4vu 159 19 ability ability NN cord-341342-kyavg4vu 159 20 of of IN cord-341342-kyavg4vu 159 21 full full JJ cord-341342-kyavg4vu 159 22 - - HYPH cord-341342-kyavg4vu 159 23 length length NN cord-341342-kyavg4vu 159 24 N n CD cord-341342-kyavg4vu 159 25 protein protein NN cord-341342-kyavg4vu 159 26 , , , cord-341342-kyavg4vu 159 27 demon- demon- NN cord-341342-kyavg4vu 159 28 strating strate VBG cord-341342-kyavg4vu 159 29 that that IN cord-341342-kyavg4vu 159 30 at at IN cord-341342-kyavg4vu 159 31 least least JJS cord-341342-kyavg4vu 159 32 the the DT cord-341342-kyavg4vu 159 33 57 57 CD cord-341342-kyavg4vu 159 34 carboxy carboxy JJ cord-341342-kyavg4vu 159 35 - - HYPH cord-341342-kyavg4vu 159 36 terminal terminal NN cord-341342-kyavg4vu 159 37 amino amino NN cord-341342-kyavg4vu 159 38 acids acid NNS cord-341342-kyavg4vu 159 39 of of IN cord-341342-kyavg4vu 159 40 N n CD cord-341342-kyavg4vu 159 41 were be VBD cord-341342-kyavg4vu 159 42 dispensable dispensable JJ cord-341342-kyavg4vu 159 43 with with IN cord-341342-kyavg4vu 159 44 respect respect NN cord-341342-kyavg4vu 159 45 to to IN cord-341342-kyavg4vu 159 46 this this DT cord-341342-kyavg4vu 159 47 function function NN cord-341342-kyavg4vu 159 48 . . . cord-341342-kyavg4vu 160 1 However however RB cord-341342-kyavg4vu 160 2 , , , cord-341342-kyavg4vu 160 3 carboxy carboxy JJ cord-341342-kyavg4vu 160 4 - - HYPH cord-341342-kyavg4vu 160 5 terminal terminal NN cord-341342-kyavg4vu 160 6 truncation truncation NN cord-341342-kyavg4vu 160 7 further far RBR cord-341342-kyavg4vu 160 8 into into IN cord-341342-kyavg4vu 160 9 the the DT cord-341342-kyavg4vu 160 10 N n CD cord-341342-kyavg4vu 160 11 molecule molecule NN cord-341342-kyavg4vu 160 12 , , , cord-341342-kyavg4vu 160 13 in in IN cord-341342-kyavg4vu 160 14 the the DT cord-341342-kyavg4vu 160 15 N N NNP cord-341342-kyavg4vu 160 16 / / SYM cord-341342-kyavg4vu 160 17 Bsm Bsm NNP cord-341342-kyavg4vu 160 18 , , , cord-341342-kyavg4vu 160 19 N N NNP cord-341342-kyavg4vu 160 20 / / SYM cord-341342-kyavg4vu 160 21 Sca Sca NNP cord-341342-kyavg4vu 160 22 , , , cord-341342-kyavg4vu 160 23 N N NNP cord-341342-kyavg4vu 160 24 / / SYM cord-341342-kyavg4vu 160 25 Eco Eco NNP cord-341342-kyavg4vu 160 26 and and CC cord-341342-kyavg4vu 160 27 N N NNP cord-341342-kyavg4vu 160 28 / / SYM cord-341342-kyavg4vu 160 29 Spe Spe NNP cord-341342-kyavg4vu 160 30 mutants mutant NNS cord-341342-kyavg4vu 160 31 , , , cord-341342-kyavg4vu 160 32 generally generally RB cord-341342-kyavg4vu 160 33 abolished abolish VBD cord-341342-kyavg4vu 160 34 the the DT cord-341342-kyavg4vu 160 35 ability ability NN cord-341342-kyavg4vu 160 36 of of IN cord-341342-kyavg4vu 160 37 N N NNP cord-341342-kyavg4vu 160 38 to to TO cord-341342-kyavg4vu 160 39 bind bind VB cord-341342-kyavg4vu 160 40 to to IN cord-341342-kyavg4vu 160 41 RNA RNA NNP cord-341342-kyavg4vu 160 42 . . . cord-341342-kyavg4vu 161 1 The the DT cord-341342-kyavg4vu 161 2 relative relative JJ cord-341342-kyavg4vu 161 3 RNA rna NN cord-341342-kyavg4vu 161 4 - - HYPH cord-341342-kyavg4vu 161 5 binding bind VBG cord-341342-kyavg4vu 161 6 ability ability NN cord-341342-kyavg4vu 161 7 of of IN cord-341342-kyavg4vu 161 8 N N NNP cord-341342-kyavg4vu 161 9 / / SYM cord-341342-kyavg4vu 161 10 Alb4 Alb4 NNP cord-341342-kyavg4vu 161 11 was be VBD cord-341342-kyavg4vu 161 12 intermediate intermediate JJ cord-341342-kyavg4vu 161 13 between between IN cord-341342-kyavg4vu 161 14 these these DT cord-341342-kyavg4vu 161 15 latter latter JJ cord-341342-kyavg4vu 161 16 and and CC cord-341342-kyavg4vu 161 17 that that DT cord-341342-kyavg4vu 161 18 of of IN cord-341342-kyavg4vu 161 19 N N NNP cord-341342-kyavg4vu 161 20 / / SYM cord-341342-kyavg4vu 161 21 Acc acc NN cord-341342-kyavg4vu 161 22 . . . cord-341342-kyavg4vu 162 1 Thus thus RB cord-341342-kyavg4vu 162 2 , , , cord-341342-kyavg4vu 162 3 the the DT cord-341342-kyavg4vu 162 4 left left JJ cord-341342-kyavg4vu 162 5 end end NN cord-341342-kyavg4vu 162 6 of of IN cord-341342-kyavg4vu 162 7 the the DT cord-341342-kyavg4vu 162 8 Albany-4 Albany-4 NNP cord-341342-kyavg4vu 162 9 deletion deletion NN cord-341342-kyavg4vu 162 10 may may MD cord-341342-kyavg4vu 162 11 define define VB cord-341342-kyavg4vu 162 12 the the DT cord-341342-kyavg4vu 162 13 right right RB cord-341342-kyavg4vu 162 14 - - HYPH cord-341342-kyavg4vu 162 15 most most JJS cord-341342-kyavg4vu 162 16 boundary boundary NN cord-341342-kyavg4vu 162 17 of of IN cord-341342-kyavg4vu 162 18 the the DT cord-341342-kyavg4vu 162 19 RNA RNA NNP cord-341342-kyavg4vu 162 20 - - HYPH cord-341342-kyavg4vu 162 21 binding bind VBG cord-341342-kyavg4vu 162 22 domain domain NN cord-341342-kyavg4vu 162 23 of of IN cord-341342-kyavg4vu 162 24 the the DT cord-341342-kyavg4vu 162 25 MHV MHV NNP cord-341342-kyavg4vu 162 26 N N NNP cord-341342-kyavg4vu 162 27 protein protein NN cord-341342-kyavg4vu 162 28 , , , cord-341342-kyavg4vu 162 29 since since IN cord-341342-kyavg4vu 162 30 the the DT cord-341342-kyavg4vu 162 31 N N NNP cord-341342-kyavg4vu 162 32 / / SYM cord-341342-kyavg4vu 162 33 Bsm Bsm NNP cord-341342-kyavg4vu 162 34 mutant mutant NN cord-341342-kyavg4vu 162 35 , , , cord-341342-kyavg4vu 162 36 which which WDT cord-341342-kyavg4vu 162 37 truncated truncate VBD cord-341342-kyavg4vu 162 38 two two CD cord-341342-kyavg4vu 162 39 further further JJ cord-341342-kyavg4vu 162 40 residues residue NNS cord-341342-kyavg4vu 162 41 , , , cord-341342-kyavg4vu 162 42 lost lose VBD cord-341342-kyavg4vu 162 43 RNA rna NN cord-341342-kyavg4vu 162 44 - - HYPH cord-341342-kyavg4vu 162 45 binding bind VBG cord-341342-kyavg4vu 162 46 ability ability NN cord-341342-kyavg4vu 162 47 . . . cord-341342-kyavg4vu 163 1 Effect effect NN cord-341342-kyavg4vu 163 2 of of IN cord-341342-kyavg4vu 163 3 amino amino NN cord-341342-kyavg4vu 163 4 - - HYPH cord-341342-kyavg4vu 163 5 terminal terminal NN cord-341342-kyavg4vu 163 6 or or CC cord-341342-kyavg4vu 163 7 centrally centrally RB cord-341342-kyavg4vu 163 8 located locate VBN cord-341342-kyavg4vu 163 9 deletions deletion NNS cord-341342-kyavg4vu 163 10 on on IN cord-341342-kyavg4vu 163 11 the the DT cord-341342-kyavg4vu 163 12 ability ability NN cord-341342-kyavg4vu 163 13 of of IN cord-341342-kyavg4vu 163 14 N n CD cord-341342-kyavg4vu 163 15 protein protein NN cord-341342-kyavg4vu 163 16 to to TO cord-341342-kyavg4vu 163 17 bind bind VB cord-341342-kyavg4vu 163 18 to to IN cord-341342-kyavg4vu 163 19 R r NN cord-341342-kyavg4vu 163 20 N n NN cord-341342-kyavg4vu 163 21 A a NN cord-341342-kyavg4vu 164 1 A a DT cord-341342-kyavg4vu 164 2 set set NN cord-341342-kyavg4vu 164 3 of of IN cord-341342-kyavg4vu 164 4 deletion deletion NN cord-341342-kyavg4vu 164 5 mutants mutant NNS cord-341342-kyavg4vu 164 6 collectively collectively RB cord-341342-kyavg4vu 164 7 spanning span VBG cord-341342-kyavg4vu 164 8 the the DT cord-341342-kyavg4vu 164 9 remainder remainder NN cord-341342-kyavg4vu 164 10 of of IN cord-341342-kyavg4vu 164 11 the the DT cord-341342-kyavg4vu 164 12 N N NNP cord-341342-kyavg4vu 164 13 molecule molecule NN cord-341342-kyavg4vu 164 14 was be VBD cord-341342-kyavg4vu 164 15 next next RB cord-341342-kyavg4vu 164 16 constructed construct VBN cord-341342-kyavg4vu 164 17 . . . cord-341342-kyavg4vu 165 1 Due due IN cord-341342-kyavg4vu 165 2 to to IN cord-341342-kyavg4vu 165 3 the the DT cord-341342-kyavg4vu 165 4 paucity paucity NN cord-341342-kyavg4vu 165 5 of of IN cord-341342-kyavg4vu 165 6 convenient convenient JJ cord-341342-kyavg4vu 165 7 restriction restriction NN cord-341342-kyavg4vu 165 8 sites site NNS cord-341342-kyavg4vu 165 9 near near IN cord-341342-kyavg4vu 165 10 P. P. NNP cord-341342-kyavg4vu 165 11 S. S. NNP cord-341342-kyavg4vu 165 12 Masters Masters NNP cord-341342-kyavg4vu 165 13 Defined define VBN cord-341342-kyavg4vu 165 14 as as IN cord-341342-kyavg4vu 165 15 the the DT cord-341342-kyavg4vu 165 16 average average JJ cord-341342-kyavg4vu 165 17 ratio ratio NN cord-341342-kyavg4vu 165 18 of of IN cord-341342-kyavg4vu 165 19 cpm cpm NNP cord-341342-kyavg4vu 165 20 of of IN cord-341342-kyavg4vu 165 21 translated translate VBN cord-341342-kyavg4vu 165 22 protein protein NN cord-341342-kyavg4vu 165 23 able able JJ cord-341342-kyavg4vu 165 24 to to TO cord-341342-kyavg4vu 165 25 enter enter VB cord-341342-kyavg4vu 165 26 a a DT cord-341342-kyavg4vu 165 27 nondenaturing nondenature VBG cord-341342-kyavg4vu 165 28 gel gel NN cord-341342-kyavg4vu 165 29 compared compare VBN cord-341342-kyavg4vu 165 30 to to IN cord-341342-kyavg4vu 165 31 cpm cpm NNP cord-341342-kyavg4vu 165 32 of of IN cord-341342-kyavg4vu 165 33 an an DT cord-341342-kyavg4vu 165 34 identical identical JJ cord-341342-kyavg4vu 165 35 sample sample NN cord-341342-kyavg4vu 165 36 binding binding NN cord-341342-kyavg4vu 165 37 in in IN cord-341342-kyavg4vu 165 38 an an DT cord-341342-kyavg4vu 165 39 SDS SDS NNP cord-341342-kyavg4vu 165 40 gel gel NN cord-341342-kyavg4vu 165 41 . . . cord-341342-kyavg4vu 166 1 The the DT cord-341342-kyavg4vu 166 2 number number NN cord-341342-kyavg4vu 166 3 of of IN cord-341342-kyavg4vu 166 4 independent independent JJ cord-341342-kyavg4vu 166 5 determinations determination NNS cord-341342-kyavg4vu 166 6 from from IN cord-341342-kyavg4vu 166 7 which which WDT cord-341342-kyavg4vu 166 8 the the DT cord-341342-kyavg4vu 166 9 average average NN cord-341342-kyavg4vu 166 10 was be VBD cord-341342-kyavg4vu 166 11 obtained obtain VBN cord-341342-kyavg4vu 166 12 is be VBZ cord-341342-kyavg4vu 166 13 indicated indicate VBN cord-341342-kyavg4vu 166 14 in in IN cord-341342-kyavg4vu 166 15 parentheses parenthesis NNS cord-341342-kyavg4vu 166 16 b b . cord-341342-kyavg4vu 167 1 For for IN cord-341342-kyavg4vu 167 2 the the DT cord-341342-kyavg4vu 167 3 carboxy carboxy JJ cord-341342-kyavg4vu 167 4 - - HYPH cord-341342-kyavg4vu 167 5 terminal terminal NN cord-341342-kyavg4vu 167 6 and and CC cord-341342-kyavg4vu 167 7 internal internal JJ cord-341342-kyavg4vu 167 8 deletions deletion NNS cord-341342-kyavg4vu 167 9 ( ( -LRB- cord-341342-kyavg4vu 167 10 upper upper JJ cord-341342-kyavg4vu 167 11 set set NN cord-341342-kyavg4vu 167 12 ) ) -RRB- cord-341342-kyavg4vu 167 13 , , , cord-341342-kyavg4vu 167 14 this this DT cord-341342-kyavg4vu 167 15 is be VBZ cord-341342-kyavg4vu 167 16 calculated calculate VBN cord-341342-kyavg4vu 167 17 relative relative JJ cord-341342-kyavg4vu 167 18 to to IN cord-341342-kyavg4vu 167 19 full full JJ cord-341342-kyavg4vu 167 20 - - HYPH cord-341342-kyavg4vu 167 21 length length NN cord-341342-kyavg4vu 167 22 MHV MHV NNP cord-341342-kyavg4vu 167 23 N N NNP cord-341342-kyavg4vu 167 24 protein protein NN cord-341342-kyavg4vu 167 25 . . . cord-341342-kyavg4vu 168 1 For for IN cord-341342-kyavg4vu 168 2 the the DT cord-341342-kyavg4vu 168 3 VSV VSV NNP cord-341342-kyavg4vu 168 4 - - HYPH cord-341342-kyavg4vu 168 5 N N NNP cord-341342-kyavg4vu 168 6 : : : cord-341342-kyavg4vu 168 7 MHV MHV NNP cord-341342-kyavg4vu 168 8 - - HYPH cord-341342-kyavg4vu 168 9 N n CD cord-341342-kyavg4vu 168 10 fusion fusion NN cord-341342-kyavg4vu 168 11 constructs construct NNS cord-341342-kyavg4vu 168 12 ( ( -LRB- cord-341342-kyavg4vu 168 13 lower low JJR cord-341342-kyavg4vu 168 14 set set NN cord-341342-kyavg4vu 168 15 ) ) -RRB- cord-341342-kyavg4vu 168 16 , , , cord-341342-kyavg4vu 168 17 this this DT cord-341342-kyavg4vu 168 18 is be VBZ cord-341342-kyavg4vu 168 19 calculated calculate VBN cord-341342-kyavg4vu 168 20 relative relative JJ cord-341342-kyavg4vu 168 21 to to IN cord-341342-kyavg4vu 168 22 the the DT cord-341342-kyavg4vu 168 23 fulMength fulmength NN cord-341342-kyavg4vu 168 24 construct construct NN cord-341342-kyavg4vu 168 25 N n CD cord-341342-kyavg4vu 168 26 / / SYM cord-341342-kyavg4vu 168 27 fusl fusl VBZ cord-341342-kyavg4vu 168 28 the the DT cord-341342-kyavg4vu 168 29 5 5 CD cord-341342-kyavg4vu 168 30 ' ' NN cord-341342-kyavg4vu 168 31 end end NN cord-341342-kyavg4vu 168 32 of of IN cord-341342-kyavg4vu 168 33 the the DT cord-341342-kyavg4vu 168 34 MHV MHV NNP cord-341342-kyavg4vu 168 35 N N NNP cord-341342-kyavg4vu 168 36 gene gene NN cord-341342-kyavg4vu 168 37 , , , cord-341342-kyavg4vu 168 38 a a DT cord-341342-kyavg4vu 168 39 fusion fusion NN cord-341342-kyavg4vu 168 40 construct construct NN cord-341342-kyavg4vu 168 41 incorporating incorporate VBG cord-341342-kyavg4vu 168 42 a a DT cord-341342-kyavg4vu 168 43 portion portion NN cord-341342-kyavg4vu 168 44 of of IN cord-341342-kyavg4vu 168 45 the the DT cord-341342-kyavg4vu 168 46 5 5 CD cord-341342-kyavg4vu 168 47 ' ' NN cord-341342-kyavg4vu 168 48 end end NN cord-341342-kyavg4vu 168 49 of of IN cord-341342-kyavg4vu 168 50 a a DT cord-341342-kyavg4vu 168 51 mutant mutant JJ cord-341342-kyavg4vu 168 52 VSV VSV NNP cord-341342-kyavg4vu 168 53 N N NNP cord-341342-kyavg4vu 168 54 gene gene NN cord-341342-kyavg4vu 168 55 was be VBD cord-341342-kyavg4vu 168 56 created create VBN cord-341342-kyavg4vu 168 57 in in IN cord-341342-kyavg4vu 168 58 order order NN cord-341342-kyavg4vu 168 59 to to TO cord-341342-kyavg4vu 168 60 provide provide VB cord-341342-kyavg4vu 168 61 a a DT cord-341342-kyavg4vu 168 62 start start NN cord-341342-kyavg4vu 168 63 codon codon NN cord-341342-kyavg4vu 168 64 for for IN cord-341342-kyavg4vu 168 65 a a DT cord-341342-kyavg4vu 168 66 construct construct NN cord-341342-kyavg4vu 168 67 in in IN cord-341342-kyavg4vu 168 68 which which WDT cord-341342-kyavg4vu 168 69 the the DT cord-341342-kyavg4vu 168 70 5 5 CD cord-341342-kyavg4vu 168 71 ' ' NN cord-341342-kyavg4vu 168 72 end end NN cord-341342-kyavg4vu 168 73 of of IN cord-341342-kyavg4vu 168 74 the the DT cord-341342-kyavg4vu 168 75 M M NNP cord-341342-kyavg4vu 168 76 H H NNP cord-341342-kyavg4vu 168 77 V v NN cord-341342-kyavg4vu 168 78 N n NN cord-341342-kyavg4vu 168 79 gene gene NN cord-341342-kyavg4vu 168 80 was be VBD cord-341342-kyavg4vu 168 81 then then RB cord-341342-kyavg4vu 168 82 removed remove VBN cord-341342-kyavg4vu 168 83 . . . cord-341342-kyavg4vu 169 1 Consequently consequently RB cord-341342-kyavg4vu 169 2 , , , cord-341342-kyavg4vu 169 3 both both DT cord-341342-kyavg4vu 169 4 N n CD cord-341342-kyavg4vu 169 5 fusion fusion NN cord-341342-kyavg4vu 169 6 genes gene NNS cord-341342-kyavg4vu 169 7 encoded encode VBD cord-341342-kyavg4vu 169 8 proteins protein NNS cord-341342-kyavg4vu 169 9 initiating initiate VBG cord-341342-kyavg4vu 169 10 with with IN cord-341342-kyavg4vu 169 11 the the DT cord-341342-kyavg4vu 169 12 VSVderived VSVderived NNP cord-341342-kyavg4vu 169 13 sequence sequence NN cord-341342-kyavg4vu 169 14 : : : cord-341342-kyavg4vu 170 1 M M NNP cord-341342-kyavg4vu 170 2 A A NNP cord-341342-kyavg4vu 171 1 P p NN cord-341342-kyavg4vu 171 2 T t NN cord-341342-kyavg4vu 171 3 V v NN cord-341342-kyavg4vu 172 1 K K NNP cord-341342-kyavg4vu 172 2 R r NN cord-341342-kyavg4vu 173 1 I -PRON- PRP cord-341342-kyavg4vu 174 1 I -PRON- PRP cord-341342-kyavg4vu 174 2 N n NN cord-341342-kyavg4vu 174 3 D d NN cord-341342-kyavg4vu 174 4 S s NN cord-341342-kyavg4vu 175 1 I -PRON- PRP cord-341342-kyavg4vu 176 1 I -PRON- PRP cord-341342-kyavg4vu 177 1 Q Q NNP cord-341342-kyavg4vu 178 1 P p NN cord-341342-kyavg4vu 179 1 K K NNP cord-341342-kyavg4vu 180 1 L l NN cord-341342-kyavg4vu 180 2 P p NN cord-341342-kyavg4vu 181 1 A a DT cord-341342-kyavg4vu 182 1 N n NN cord-341342-kyavg4vu 182 2 E e NN cord-341342-kyavg4vu 182 3 D d NN cord-341342-kyavg4vu 182 4 P p NN cord-341342-kyavg4vu 182 5 D d NN cord-341342-kyavg4vu 183 1 R R NNP cord-341342-kyavg4vu 183 2 S s NN cord-341342-kyavg4vu 184 1 A a DT cord-341342-kyavg4vu 184 2 E e NN cord-341342-kyavg4vu 184 3 D d NN cord-341342-kyavg4vu 184 4 D d NN cord-341342-kyavg4vu 184 5 K k NN cord-341342-kyavg4vu 184 6 W w NN cord-341342-kyavg4vu 184 7 L l NN cord-341342-kyavg4vu 184 8 P p NN cord-341342-kyavg4vu 185 1 I -PRON- PRP cord-341342-kyavg4vu 185 2 -YILG -YILG NNP cord-341342-kyavg4vu 185 3 , , , cord-341342-kyavg4vu 185 4 corresponding correspond VBG cord-341342-kyavg4vu 185 5 to to IN cord-341342-kyavg4vu 185 6 amino amino JJ cord-341342-kyavg4vu 185 7 acids acid NNS cord-341342-kyavg4vu 185 8 1 1 CD cord-341342-kyavg4vu 185 9 - - SYM cord-341342-kyavg4vu 185 10 23 23 CD cord-341342-kyavg4vu 185 11 and and CC cord-341342-kyavg4vu 185 12 124 124 CD cord-341342-kyavg4vu 185 13 - - SYM cord-341342-kyavg4vu 185 14 140 140 CD cord-341342-kyavg4vu 185 15 of of IN cord-341342-kyavg4vu 185 16 that that DT cord-341342-kyavg4vu 185 17 protein protein NN cord-341342-kyavg4vu 185 18 [ [ -LRB- cord-341342-kyavg4vu 185 19 16 16 CD cord-341342-kyavg4vu 185 20 ] ] -RRB- cord-341342-kyavg4vu 185 21 . . . cord-341342-kyavg4vu 186 1 The the DT cord-341342-kyavg4vu 186 2 full full JJ cord-341342-kyavg4vu 186 3 - - HYPH cord-341342-kyavg4vu 186 4 length length NN cord-341342-kyavg4vu 186 5 fusion fusion NN cord-341342-kyavg4vu 186 6 protein protein NN cord-341342-kyavg4vu 186 7 , , , cord-341342-kyavg4vu 186 8 designated designated JJ cord-341342-kyavg4vu 186 9 N N NNP cord-341342-kyavg4vu 186 10 / / SYM cord-341342-kyavg4vu 186 11 fusl fusl NNS cord-341342-kyavg4vu 186 12 , , , cord-341342-kyavg4vu 186 13 contained contain VBD cord-341342-kyavg4vu 186 14 the the DT cord-341342-kyavg4vu 186 15 entire entire JJ cord-341342-kyavg4vu 186 16 M M NNP cord-341342-kyavg4vu 186 17 H h NN cord-341342-kyavg4vu 186 18 V v NN cord-341342-kyavg4vu 186 19 N n NN cord-341342-kyavg4vu 186 20 protein protein NN cord-341342-kyavg4vu 186 21 following follow VBG cord-341342-kyavg4vu 186 22 this this DT cord-341342-kyavg4vu 186 23 sequence sequence NN cord-341342-kyavg4vu 186 24 . . . cord-341342-kyavg4vu 187 1 The the DT cord-341342-kyavg4vu 187 2 amino amino NN cord-341342-kyavg4vu 187 3 - - HYPH cord-341342-kyavg4vu 187 4 terminal terminal NN cord-341342-kyavg4vu 187 5 deletion deletion NN cord-341342-kyavg4vu 187 6 mutant mutant NN cord-341342-kyavg4vu 187 7 , , , cord-341342-kyavg4vu 187 8 N/ N/ NNP cord-341342-kyavg4vu 187 9 fus2 fus2 NNP cord-341342-kyavg4vu 187 10 , , , cord-341342-kyavg4vu 187 11 lacking lack VBG cord-341342-kyavg4vu 187 12 the the DT cord-341342-kyavg4vu 187 13 first first JJ cord-341342-kyavg4vu 187 14 135 135 CD cord-341342-kyavg4vu 187 15 amino amino NN cord-341342-kyavg4vu 187 16 acids acid NNS cord-341342-kyavg4vu 187 17 of of IN cord-341342-kyavg4vu 187 18 the the DT cord-341342-kyavg4vu 187 19 MHV MHV NNP cord-341342-kyavg4vu 187 20 N N NNP cord-341342-kyavg4vu 187 21 protein protein NN cord-341342-kyavg4vu 187 22 , , , cord-341342-kyavg4vu 187 23 contained contain VBD cord-341342-kyavg4vu 187 24 the the DT cord-341342-kyavg4vu 187 25 VSV VSV NNP cord-341342-kyavg4vu 187 26 sequence sequence NN cord-341342-kyavg4vu 187 27 fused fuse VBN cord-341342-kyavg4vu 187 28 to to IN cord-341342-kyavg4vu 187 29 the the DT cord-341342-kyavg4vu 187 30 remainder remainder NN cord-341342-kyavg4vu 187 31 of of IN cord-341342-kyavg4vu 187 32 MHV MHV NNP cord-341342-kyavg4vu 187 33 N. N. NNP cord-341342-kyavg4vu 187 34 Two two CD cord-341342-kyavg4vu 187 35 internal internal JJ cord-341342-kyavg4vu 187 36 deletions deletion NNS cord-341342-kyavg4vu 187 37 mutants mutant NNS cord-341342-kyavg4vu 187 38 , , , cord-341342-kyavg4vu 187 39 encoding encode VBG cord-341342-kyavg4vu 187 40 N n CD cord-341342-kyavg4vu 187 41 / / SYM cord-341342-kyavg4vu 187 42 Apa Apa NNP cord-341342-kyavg4vu 187 43 - - HYPH cord-341342-kyavg4vu 187 44 Nhe Nhe NNP cord-341342-kyavg4vu 187 45 and and CC cord-341342-kyavg4vu 187 46 N N NNP cord-341342-kyavg4vu 187 47 / / SYM cord-341342-kyavg4vu 187 48 Nhe Nhe NNP cord-341342-kyavg4vu 187 49 - - HYPH cord-341342-kyavg4vu 187 50 Spe Spe NNP cord-341342-kyavg4vu 187 51 , , , cord-341342-kyavg4vu 187 52 were be VBD cord-341342-kyavg4vu 187 53 generated generate VBN cord-341342-kyavg4vu 187 54 by by IN cord-341342-kyavg4vu 187 55 removal removal NN cord-341342-kyavg4vu 187 56 of of IN cord-341342-kyavg4vu 187 57 the the DT cord-341342-kyavg4vu 187 58 indicated indicate VBN cord-341342-kyavg4vu 187 59 restriction restriction NN cord-341342-kyavg4vu 187 60 fragments fragment NNS cord-341342-kyavg4vu 187 61 from from IN cord-341342-kyavg4vu 187 62 one one CD cord-341342-kyavg4vu 187 63 of of IN cord-341342-kyavg4vu 187 64 the the DT cord-341342-kyavg4vu 187 65 original original JJ cord-341342-kyavg4vu 187 66 ( ( -LRB- cord-341342-kyavg4vu 187 67 nonfusion nonfusion NN cord-341342-kyavg4vu 187 68 ) ) -RRB- cord-341342-kyavg4vu 187 69 N n CD cord-341342-kyavg4vu 187 70 vectors vector NNS cord-341342-kyavg4vu 187 71 ( ( -LRB- cord-341342-kyavg4vu 187 72 Fig Fig NNP cord-341342-kyavg4vu 187 73 . . . cord-341342-kyavg4vu 187 74 1 1 CD cord-341342-kyavg4vu 187 75 ) ) -RRB- cord-341342-kyavg4vu 187 76 . . . cord-341342-kyavg4vu 188 1 The the DT cord-341342-kyavg4vu 188 2 resulting result VBG cord-341342-kyavg4vu 188 3 protein protein NN cord-341342-kyavg4vu 188 4 products product NNS cord-341342-kyavg4vu 188 5 transcribed transcribe VBN cord-341342-kyavg4vu 188 6 and and CC cord-341342-kyavg4vu 188 7 translated translate VBN cord-341342-kyavg4vu 188 8 from from IN cord-341342-kyavg4vu 188 9 these these DT cord-341342-kyavg4vu 188 10 lacked lack VBN cord-341342-kyavg4vu 188 11 regions region NNS cord-341342-kyavg4vu 188 12 of of IN cord-341342-kyavg4vu 188 13 66 66 CD cord-341342-kyavg4vu 188 14 and and CC cord-341342-kyavg4vu 188 15 106 106 CD cord-341342-kyavg4vu 188 16 amino amino JJ cord-341342-kyavg4vu 188 17 acids acid NNS cord-341342-kyavg4vu 188 18 , , , cord-341342-kyavg4vu 188 19 respectively respectively RB cord-341342-kyavg4vu 188 20 . . . cord-341342-kyavg4vu 189 1 These these DT cord-341342-kyavg4vu 189 2 mutants mutant NNS cord-341342-kyavg4vu 189 3 were be VBD cord-341342-kyavg4vu 189 4 analyzed analyze VBN cord-341342-kyavg4vu 189 5 by by IN cord-341342-kyavg4vu 189 6 the the DT cord-341342-kyavg4vu 189 7 two two CD cord-341342-kyavg4vu 189 8 gel gel NN cord-341342-kyavg4vu 189 9 electrophoresis electrophoresis NN cord-341342-kyavg4vu 189 10 systems system NNS cord-341342-kyavg4vu 189 11 and and CC cord-341342-kyavg4vu 189 12 on on IN cord-341342-kyavg4vu 189 13 SDS SDS NNP cord-341342-kyavg4vu 189 14 - - HYPH cord-341342-kyavg4vu 189 15 PAGE PAGE NNP cord-341342-kyavg4vu 189 16 were be VBD cord-341342-kyavg4vu 189 17 observed observe VBN cord-341342-kyavg4vu 189 18 to to TO cord-341342-kyavg4vu 189 19 be be VB cord-341342-kyavg4vu 189 20 homogeneous homogeneous JJ cord-341342-kyavg4vu 189 21 and and CC cord-341342-kyavg4vu 189 22 of of IN cord-341342-kyavg4vu 189 23 the the DT cord-341342-kyavg4vu 189 24 anticipated anticipated JJ cord-341342-kyavg4vu 189 25 relative relative JJ cord-341342-kyavg4vu 189 26 mobilities mobility NNS cord-341342-kyavg4vu 189 27 ( ( -LRB- cord-341342-kyavg4vu 189 28 Fig Fig NNP cord-341342-kyavg4vu 189 29 . . NNP cord-341342-kyavg4vu 189 30 6 6 CD cord-341342-kyavg4vu 189 31 , , , cord-341342-kyavg4vu 189 32 lanes lane VBZ cord-341342-kyavg4vu 189 33 a a DT cord-341342-kyavg4vu 189 34 to to IN cord-341342-kyavg4vu 189 35 k k NNP cord-341342-kyavg4vu 189 36 ) ) -RRB- cord-341342-kyavg4vu 189 37 . . . cord-341342-kyavg4vu 190 1 Nondenaturing Nondenaturing NNP cord-341342-kyavg4vu 190 2 PAGE PAGE NNP cord-341342-kyavg4vu 190 3 revealed reveal VBD cord-341342-kyavg4vu 190 4 that that IN cord-341342-kyavg4vu 190 5 the the DT cord-341342-kyavg4vu 190 6 N N NNP cord-341342-kyavg4vu 190 7 / / SYM cord-341342-kyavg4vu 190 8 fus fus NNP cord-341342-kyavg4vu 190 9 1 1 CD cord-341342-kyavg4vu 190 10 and and CC cord-341342-kyavg4vu 190 11 N N NNP cord-341342-kyavg4vu 190 12 / / SYM cord-341342-kyavg4vu 190 13 fus2 fus2 NNP cord-341342-kyavg4vu 190 14 proteins protein NNS cord-341342-kyavg4vu 190 15 retained retain VBD cord-341342-kyavg4vu 190 16 the the DT cord-341342-kyavg4vu 190 17 ability ability NN cord-341342-kyavg4vu 190 18 to to TO cord-341342-kyavg4vu 190 19 bind bind VB cord-341342-kyavg4vu 190 20 to to IN cord-341342-kyavg4vu 190 21 RNA RNA NNP cord-341342-kyavg4vu 190 22 , , , cord-341342-kyavg4vu 190 23 whereas whereas IN cord-341342-kyavg4vu 190 24 the the DT cord-341342-kyavg4vu 190 25 deletions deletion NNS cord-341342-kyavg4vu 190 26 in in IN cord-341342-kyavg4vu 190 27 N n CD cord-341342-kyavg4vu 190 28 / / SYM cord-341342-kyavg4vu 190 29 Apa Apa NNP cord-341342-kyavg4vu 190 30 - - HYPH cord-341342-kyavg4vu 190 31 Nhe Nhe NNP cord-341342-kyavg4vu 190 32 and and CC cord-341342-kyavg4vu 190 33 N N NNP cord-341342-kyavg4vu 190 34 / / SYM cord-341342-kyavg4vu 190 35 Nhe Nhe NNP cord-341342-kyavg4vu 190 36 - - HYPH cord-341342-kyavg4vu 190 37 Spe Spe NNP cord-341342-kyavg4vu 190 38 abolished abolish VBD cord-341342-kyavg4vu 190 39 RNA RNA NNP cord-341342-kyavg4vu 190 40 binding bind VBG cord-341342-kyavg4vu 190 41 ( ( -LRB- cord-341342-kyavg4vu 190 42 Fig Fig NNP cord-341342-kyavg4vu 190 43 . . NNP cord-341342-kyavg4vu 190 44 6 6 CD cord-341342-kyavg4vu 190 45 , , , cord-341342-kyavg4vu 190 46 lanes lane VBZ cord-341342-kyavg4vu 190 47 a a DT cord-341342-kyavg4vu 190 48 ' ' '' cord-341342-kyavg4vu 190 49 to to IN cord-341342-kyavg4vu 190 50 k k NNP cord-341342-kyavg4vu 190 51 ' ' '' cord-341342-kyavg4vu 190 52 ) ) -RRB- cord-341342-kyavg4vu 190 53 . . . cord-341342-kyavg4vu 191 1 Quantitation quantitation NN cord-341342-kyavg4vu 191 2 of of IN cord-341342-kyavg4vu 191 3 the the DT cord-341342-kyavg4vu 191 4 extent extent NN cord-341342-kyavg4vu 191 5 of of IN cord-341342-kyavg4vu 191 6 RNA RNA NNP cord-341342-kyavg4vu 191 7 binding binding NN cord-341342-kyavg4vu 191 8 showed show VBD cord-341342-kyavg4vu 191 9 that that IN cord-341342-kyavg4vu 191 10 the the DT cord-341342-kyavg4vu 191 11 N N NNP cord-341342-kyavg4vu 191 12 / / SYM cord-341342-kyavg4vu 191 13 fusl fusl NNS cord-341342-kyavg4vu 191 14 mutant mutant NN cord-341342-kyavg4vu 191 15 was be VBD cord-341342-kyavg4vu 191 16 62 62 CD cord-341342-kyavg4vu 191 17 % % NN cord-341342-kyavg4vu 191 18 as as IN cord-341342-kyavg4vu 191 19 binding binding NN cord-341342-kyavg4vu 191 20 - - HYPH cord-341342-kyavg4vu 191 21 competent competent JJ cord-341342-kyavg4vu 191 22 as as IN cord-341342-kyavg4vu 191 23 unaltered unaltered JJ cord-341342-kyavg4vu 191 24 , , , cord-341342-kyavg4vu 191 25 full full JJ cord-341342-kyavg4vu 191 26 - - HYPH cord-341342-kyavg4vu 191 27 length length NN cord-341342-kyavg4vu 192 1 N n NN cord-341342-kyavg4vu 192 2 protein protein NN cord-341342-kyavg4vu 192 3 ( ( -LRB- cord-341342-kyavg4vu 192 4 Table table NN cord-341342-kyavg4vu 192 5 1 1 CD cord-341342-kyavg4vu 192 6 ) ) -RRB- cord-341342-kyavg4vu 192 7 . . . cord-341342-kyavg4vu 193 1 Although although IN cord-341342-kyavg4vu 193 2 the the DT cord-341342-kyavg4vu 193 3 VSV VSV NNP cord-341342-kyavg4vu 193 4 N N NNP cord-341342-kyavg4vu 193 5 protein protein NN cord-341342-kyavg4vu 193 6 is be VBZ cord-341342-kyavg4vu 193 7 , , , cord-341342-kyavg4vu 193 8 itself -PRON- PRP cord-341342-kyavg4vu 193 9 , , , cord-341342-kyavg4vu 193 10 an an DT cord-341342-kyavg4vu 193 11 RNA rna NN cord-341342-kyavg4vu 193 12 - - HYPH cord-341342-kyavg4vu 193 13 binding bind VBG cord-341342-kyavg4vu 193 14 protein protein NN cord-341342-kyavg4vu 193 15 , , , cord-341342-kyavg4vu 193 16 the the DT cord-341342-kyavg4vu 193 17 mutant mutant JJ cord-341342-kyavg4vu 193 18 VSV VSV NNP cord-341342-kyavg4vu 193 19 N N NNP cord-341342-kyavg4vu 193 20 protein protein NN cord-341342-kyavg4vu 193 21 used use VBN cord-341342-kyavg4vu 193 22 in in IN cord-341342-kyavg4vu 193 23 the the DT cord-341342-kyavg4vu 193 24 construction construction NN cord-341342-kyavg4vu 193 25 of of IN cord-341342-kyavg4vu 193 26 N N NNP cord-341342-kyavg4vu 193 27 / / SYM cord-341342-kyavg4vu 193 28 fus fus NNP cord-341342-kyavg4vu 193 29 1 1 CD cord-341342-kyavg4vu 193 30 and and CC cord-341342-kyavg4vu 193 31 N N NNP cord-341342-kyavg4vu 193 32 / / SYM cord-341342-kyavg4vu 193 33 fus2 fus2 NNP cord-341342-kyavg4vu 193 34 is be VBZ cord-341342-kyavg4vu 193 35 unable unable JJ cord-341342-kyavg4vu 193 36 to to TO cord-341342-kyavg4vu 193 37 bind bind VB cord-341342-kyavg4vu 193 38 Indeed indeed RB cord-341342-kyavg4vu 193 39 , , , cord-341342-kyavg4vu 193 40 for for IN cord-341342-kyavg4vu 193 41 N n CD cord-341342-kyavg4vu 193 42 / / SYM cord-341342-kyavg4vu 193 43 fus fus NNP cord-341342-kyavg4vu 193 44 1 1 VBD cord-341342-kyavg4vu 193 45 the the DT cord-341342-kyavg4vu 193 46 VSV VSV NNP cord-341342-kyavg4vu 193 47 N N NNP cord-341342-kyavg4vu 193 48 - - HYPH cord-341342-kyavg4vu 193 49 derived derive VBN cord-341342-kyavg4vu 193 50 portion portion NN cord-341342-kyavg4vu 193 51 of of IN cord-341342-kyavg4vu 193 52 the the DT cord-341342-kyavg4vu 193 53 molecule molecule NN cord-341342-kyavg4vu 193 54 appeared appear VBD cord-341342-kyavg4vu 193 55 to to TO cord-341342-kyavg4vu 193 56 somewhat somewhat RB cord-341342-kyavg4vu 193 57 impede impede VB cord-341342-kyavg4vu 193 58 the the DT cord-341342-kyavg4vu 193 59 entry entry NN cord-341342-kyavg4vu 193 60 of of IN cord-341342-kyavg4vu 193 61 MHV MHV NNP cord-341342-kyavg4vu 193 62 N N NNP cord-341342-kyavg4vu 193 63 into into IN cord-341342-kyavg4vu 193 64 the the DT cord-341342-kyavg4vu 193 65 nondenaturing nondenature VBG cord-341342-kyavg4vu 193 66 gel gel NN cord-341342-kyavg4vu 193 67 . . . cord-341342-kyavg4vu 194 1 Remarkably remarkably RB cord-341342-kyavg4vu 194 2 , , , cord-341342-kyavg4vu 194 3 the the DT cord-341342-kyavg4vu 194 4 N N NNP cord-341342-kyavg4vu 194 5 / / SYM cord-341342-kyavg4vu 194 6 fus2 fus2 NNP cord-341342-kyavg4vu 194 7 protein protein NN cord-341342-kyavg4vu 194 8 was be VBD cord-341342-kyavg4vu 194 9 a a DT cord-341342-kyavg4vu 194 10 better well JJR cord-341342-kyavg4vu 194 11 RNA rna NN cord-341342-kyavg4vu 194 12 - - HYPH cord-341342-kyavg4vu 194 13 binding bind VBG cord-341342-kyavg4vu 194 14 molecule molecule NN cord-341342-kyavg4vu 194 15 than than IN cord-341342-kyavg4vu 194 16 its -PRON- PRP$ cord-341342-kyavg4vu 194 17 parent parent NN cord-341342-kyavg4vu 194 18 , , , cord-341342-kyavg4vu 194 19 N n CD cord-341342-kyavg4vu 194 20 / / SYM cord-341342-kyavg4vu 194 21 fusl fusl NNS cord-341342-kyavg4vu 194 22 , , , cord-341342-kyavg4vu 194 23 in in IN cord-341342-kyavg4vu 194 24 spite spite NN cord-341342-kyavg4vu 194 25 of of IN cord-341342-kyavg4vu 194 26 the the DT cord-341342-kyavg4vu 194 27 loss loss NN cord-341342-kyavg4vu 194 28 of of IN cord-341342-kyavg4vu 194 29 a a DT cord-341342-kyavg4vu 194 30 large large JJ cord-341342-kyavg4vu 194 31 portion portion NN cord-341342-kyavg4vu 194 32 of of IN cord-341342-kyavg4vu 194 33 the the DT cord-341342-kyavg4vu 194 34 MHV MHV NNP cord-341342-kyavg4vu 194 35 N N NNP cord-341342-kyavg4vu 194 36 amino amino NN cord-341342-kyavg4vu 194 37 terminus terminus NN cord-341342-kyavg4vu 194 38 ( ( -LRB- cord-341342-kyavg4vu 194 39 Table table NN cord-341342-kyavg4vu 194 40 1 1 CD cord-341342-kyavg4vu 194 41 ) ) -RRB- cord-341342-kyavg4vu 194 42 . . . cord-341342-kyavg4vu 195 1 Clearly clearly RB cord-341342-kyavg4vu 195 2 , , , cord-341342-kyavg4vu 195 3 this this DT cord-341342-kyavg4vu 195 4 deleted delete VBN cord-341342-kyavg4vu 195 5 portion portion NN cord-341342-kyavg4vu 195 6 of of IN cord-341342-kyavg4vu 195 7 the the DT cord-341342-kyavg4vu 195 8 N n CD cord-341342-kyavg4vu 195 9 molecule molecule NN cord-341342-kyavg4vu 195 10 can can MD cord-341342-kyavg4vu 195 11 play play VB cord-341342-kyavg4vu 195 12 little little JJ cord-341342-kyavg4vu 195 13 or or CC cord-341342-kyavg4vu 195 14 no no DT cord-341342-kyavg4vu 195 15 role role NN cord-341342-kyavg4vu 195 16 in in IN cord-341342-kyavg4vu 195 17 RNA RNA NNP cord-341342-kyavg4vu 195 18 binding binding NN cord-341342-kyavg4vu 195 19 . . . cord-341342-kyavg4vu 196 1 By by IN cord-341342-kyavg4vu 196 2 contrast contrast NN cord-341342-kyavg4vu 196 3 , , , cord-341342-kyavg4vu 196 4 the the DT cord-341342-kyavg4vu 196 5 deletions deletion NNS cord-341342-kyavg4vu 196 6 in in IN cord-341342-kyavg4vu 196 7 the the DT cord-341342-kyavg4vu 196 8 N N NNP cord-341342-kyavg4vu 196 9 / / SYM cord-341342-kyavg4vu 196 10 Apa Apa NNP cord-341342-kyavg4vu 196 11 - - HYPH cord-341342-kyavg4vu 196 12 Nhe Nhe NNP cord-341342-kyavg4vu 196 13 and and CC cord-341342-kyavg4vu 196 14 N N NNP cord-341342-kyavg4vu 196 15 / / SYM cord-341342-kyavg4vu 196 16 Nhe Nhe NNP cord-341342-kyavg4vu 196 17 - - HYPH cord-341342-kyavg4vu 196 18 Spe Spe NNP cord-341342-kyavg4vu 196 19 mutants mutant NNS cord-341342-kyavg4vu 196 20 reduced reduce VBD cord-341342-kyavg4vu 196 21 RNA RNA NNP cord-341342-kyavg4vu 196 22 binding bind VBG cord-341342-kyavg4vu 196 23 to to IN cord-341342-kyavg4vu 196 24 residual residual JJ cord-341342-kyavg4vu 196 25 levels level NNS cord-341342-kyavg4vu 196 26 similar similar JJ cord-341342-kyavg4vu 196 27 to to IN cord-341342-kyavg4vu 196 28 those those DT cord-341342-kyavg4vu 196 29 observed observe VBN cord-341342-kyavg4vu 196 30 for for IN cord-341342-kyavg4vu 196 31 the the DT cord-341342-kyavg4vu 196 32 four four CD cord-341342-kyavg4vu 196 33 shortest short JJS cord-341342-kyavg4vu 196 34 carboxy carboxy JJ cord-341342-kyavg4vu 196 35 - - HYPH cord-341342-kyavg4vu 196 36 terminal terminal NN cord-341342-kyavg4vu 196 37 truncation truncation NN cord-341342-kyavg4vu 196 38 mutants mutant NNS cord-341342-kyavg4vu 196 39 ( ( -LRB- cord-341342-kyavg4vu 196 40 Table table NN cord-341342-kyavg4vu 196 41 1 1 CD cord-341342-kyavg4vu 196 42 ) ) -RRB- cord-341342-kyavg4vu 196 43 . . . cord-341342-kyavg4vu 197 1 Since since IN cord-341342-kyavg4vu 197 2 removal removal NN cord-341342-kyavg4vu 197 3 of of IN cord-341342-kyavg4vu 197 4 a a DT cord-341342-kyavg4vu 197 5 substantial substantial JJ cord-341342-kyavg4vu 197 6 portion portion NN cord-341342-kyavg4vu 197 7 of of IN cord-341342-kyavg4vu 197 8 either either CC cord-341342-kyavg4vu 197 9 the the DT cord-341342-kyavg4vu 197 10 amino amino NN cord-341342-kyavg4vu 197 11 or or CC cord-341342-kyavg4vu 197 12 carboxy carboxy JJ cord-341342-kyavg4vu 197 13 terminus terminus NN cord-341342-kyavg4vu 197 14 of of IN cord-341342-kyavg4vu 197 15 the the DT cord-341342-kyavg4vu 197 16 N N NNP cord-341342-kyavg4vu 197 17 molecule molecule NN cord-341342-kyavg4vu 197 18 allowed allow VBD cord-341342-kyavg4vu 197 19 it -PRON- PRP cord-341342-kyavg4vu 197 20 to to TO cord-341342-kyavg4vu 197 21 retain retain VB cord-341342-kyavg4vu 197 22 the the DT cord-341342-kyavg4vu 197 23 ability ability NN cord-341342-kyavg4vu 197 24 to to TO cord-341342-kyavg4vu 197 25 bind bind VB cord-341342-kyavg4vu 197 26 to to IN cord-341342-kyavg4vu 197 27 RNA RNA NNP cord-341342-kyavg4vu 197 28 , , , cord-341342-kyavg4vu 197 29 it -PRON- PRP cord-341342-kyavg4vu 197 30 became become VBD cord-341342-kyavg4vu 197 31 of of IN cord-341342-kyavg4vu 197 32 interest interest NN cord-341342-kyavg4vu 197 33 to to TO cord-341342-kyavg4vu 197 34 examine examine VB cord-341342-kyavg4vu 197 35 the the DT cord-341342-kyavg4vu 197 36 effect effect NN cord-341342-kyavg4vu 197 37 of of IN cord-341342-kyavg4vu 197 38 removal removal NN cord-341342-kyavg4vu 197 39 of of IN cord-341342-kyavg4vu 197 40 both both DT cord-341342-kyavg4vu 197 41 of of IN cord-341342-kyavg4vu 197 42 these these DT cord-341342-kyavg4vu 197 43 regions region NNS cord-341342-kyavg4vu 197 44 . . . cord-341342-kyavg4vu 198 1 This this DT cord-341342-kyavg4vu 198 2 was be VBD cord-341342-kyavg4vu 198 3 accomplished accomplish VBN cord-341342-kyavg4vu 198 4 by by IN cord-341342-kyavg4vu 198 5 generating generate VBG cord-341342-kyavg4vu 198 6 the the DT cord-341342-kyavg4vu 198 7 doubly doubly RB cord-341342-kyavg4vu 198 8 - - HYPH cord-341342-kyavg4vu 198 9 truncated truncate VBN cord-341342-kyavg4vu 198 10 mutant mutant JJ cord-341342-kyavg4vu 198 11 N N NNP cord-341342-kyavg4vu 198 12 / / SYM cord-341342-kyavg4vu 198 13 fus fus NNP cord-341342-kyavg4vu 198 14 1 1 NNP cord-341342-kyavg4vu 198 15 / / SYM cord-341342-kyavg4vu 198 16 Acc Acc NNP cord-341342-kyavg4vu 198 17 . . . cord-341342-kyavg4vu 199 1 Included include VBN cord-341342-kyavg4vu 199 2 for for IN cord-341342-kyavg4vu 199 3 comparison comparison NN cord-341342-kyavg4vu 199 4 in in IN cord-341342-kyavg4vu 199 5 these these DT cord-341342-kyavg4vu 199 6 experiments experiment NNS cord-341342-kyavg4vu 199 7 was be VBD cord-341342-kyavg4vu 200 1 N N NNP cord-341342-kyavg4vu 200 2 / / SYM cord-341342-kyavg4vu 200 3 fus4 fus4 NNP cord-341342-kyavg4vu 200 4 , , , cord-341342-kyavg4vu 200 5 an an DT cord-341342-kyavg4vu 200 6 N n CD cord-341342-kyavg4vu 200 7 mutant mutant NN cord-341342-kyavg4vu 200 8 containing contain VBG cord-341342-kyavg4vu 200 9 the the DT cord-341342-kyavg4vu 200 10 same same JJ cord-341342-kyavg4vu 200 11 amino amino NN cord-341342-kyavg4vu 200 12 - - HYPH cord-341342-kyavg4vu 200 13 terminal terminal NN cord-341342-kyavg4vu 200 14 deletion deletion NN cord-341342-kyavg4vu 200 15 as as IN cord-341342-kyavg4vu 200 16 N n CD cord-341342-kyavg4vu 200 17 / / SYM cord-341342-kyavg4vu 200 18 fus2 fus2 NNP cord-341342-kyavg4vu 200 19 but but CC cord-341342-kyavg4vu 200 20 having have VBG cord-341342-kyavg4vu 200 21 only only RB cord-341342-kyavg4vu 200 22 8 8 CD cord-341342-kyavg4vu 200 23 amino amino JJ cord-341342-kyavg4vu 200 24 acids acid NNS cord-341342-kyavg4vu 200 25 derived derive VBN cord-341342-kyavg4vu 200 26 from from IN cord-341342-kyavg4vu 200 27 the the DT cord-341342-kyavg4vu 200 28 VSV VSV NNP cord-341342-kyavg4vu 200 29 N N NNP cord-341342-kyavg4vu 200 30 protein protein NN cord-341342-kyavg4vu 200 31 ( ( -LRB- cord-341342-kyavg4vu 200 32 MAPTVKRI MAPTVKRI NNP cord-341342-kyavg4vu 200 33 ) ) -RRB- cord-341342-kyavg4vu 200 34 . . . cord-341342-kyavg4vu 201 1 In in IN cord-341342-kyavg4vu 201 2 addition addition NN cord-341342-kyavg4vu 201 3 , , , cord-341342-kyavg4vu 201 4 the the DT cord-341342-kyavg4vu 201 5 corresponding correspond VBG cord-341342-kyavg4vu 201 6 AccIgenerated AccIgenerated NNP cord-341342-kyavg4vu 201 7 truncation truncation NN cord-341342-kyavg4vu 201 8 of of IN cord-341342-kyavg4vu 201 9 N N NNP cord-341342-kyavg4vu 201 10 / / SYM cord-341342-kyavg4vu 201 11 fus4 fus4 NNP cord-341342-kyavg4vu 201 12 was be VBD cord-341342-kyavg4vu 201 13 examined examine VBN cord-341342-kyavg4vu 201 14 . . . cord-341342-kyavg4vu 202 1 Analysis analysis NN cord-341342-kyavg4vu 202 2 of of IN cord-341342-kyavg4vu 202 3 these these DT cord-341342-kyavg4vu 202 4 N N NNP cord-341342-kyavg4vu 202 5 protein protein NN cord-341342-kyavg4vu 202 6 mutants mutant NNS cord-341342-kyavg4vu 202 7 and and CC cord-341342-kyavg4vu 202 8 their -PRON- PRP$ cord-341342-kyavg4vu 202 9 full full JJ cord-341342-kyavg4vu 202 10 - - HYPH cord-341342-kyavg4vu 202 11 length length NN cord-341342-kyavg4vu 202 12 counterparts counterpart NNS cord-341342-kyavg4vu 202 13 demonstrated demonstrate VBD cord-341342-kyavg4vu 202 14 that that IN cord-341342-kyavg4vu 202 15 all all DT cord-341342-kyavg4vu 202 16 were be VBD cord-341342-kyavg4vu 202 17 able able JJ cord-341342-kyavg4vu 202 18 to to TO cord-341342-kyavg4vu 202 19 enter enter VB cord-341342-kyavg4vu 202 20 nondenaturing nondenature VBG cord-341342-kyavg4vu 202 21 gels gel NNS cord-341342-kyavg4vu 202 22 to to IN cord-341342-kyavg4vu 202 23 a a DT cord-341342-kyavg4vu 202 24 significant significant JJ cord-341342-kyavg4vu 202 25 degree degree NN cord-341342-kyavg4vu 202 26 ( ( -LRB- cord-341342-kyavg4vu 202 27 Fig Fig NNP cord-341342-kyavg4vu 202 28 . . NNP cord-341342-kyavg4vu 202 29 7 7 CD cord-341342-kyavg4vu 202 30 ) ) -RRB- cord-341342-kyavg4vu 202 31 . . . cord-341342-kyavg4vu 203 1 Quantitation quantitation NN cord-341342-kyavg4vu 203 2 of of IN cord-341342-kyavg4vu 203 3 the the DT cord-341342-kyavg4vu 203 4 gel gel NN cord-341342-kyavg4vu 203 5 assay assay NN cord-341342-kyavg4vu 203 6 showed show VBD cord-341342-kyavg4vu 203 7 that that IN cord-341342-kyavg4vu 203 8 N N NNP cord-341342-kyavg4vu 203 9 / / SYM cord-341342-kyavg4vu 203 10 fus4 fus4 NNP cord-341342-kyavg4vu 203 11 was be VBD cord-341342-kyavg4vu 203 12 at at IN cord-341342-kyavg4vu 203 13 least least JJS cord-341342-kyavg4vu 203 14 as as RB cord-341342-kyavg4vu 203 15 competent competent JJ cord-341342-kyavg4vu 203 16 as as IN cord-341342-kyavg4vu 203 17 full full JJ cord-341342-kyavg4vu 203 18 - - HYPH cord-341342-kyavg4vu 203 19 length length NN cord-341342-kyavg4vu 203 20 N n CD cord-341342-kyavg4vu 203 21 protein protein NN cord-341342-kyavg4vu 203 22 and and CC cord-341342-kyavg4vu 203 23 nearly nearly RB cord-341342-kyavg4vu 203 24 twice twice PDT cord-341342-kyavg4vu 203 25 as as RB cord-341342-kyavg4vu 203 26 competent competent JJ cord-341342-kyavg4vu 203 27 as as IN cord-341342-kyavg4vu 203 28 N n CD cord-341342-kyavg4vu 203 29 / / SYM cord-341342-kyavg4vu 203 30 fus fus NNP cord-341342-kyavg4vu 203 31 1 1 CD cord-341342-kyavg4vu 203 32 in in IN cord-341342-kyavg4vu 203 33 RNA RNA NNP cord-341342-kyavg4vu 203 34 binding binding NN cord-341342-kyavg4vu 203 35 ( ( -LRB- cord-341342-kyavg4vu 203 36 Table Table NNP cord-341342-kyavg4vu 203 37 1 1 CD cord-341342-kyavg4vu 203 38 ) ) -RRB- cord-341342-kyavg4vu 203 39 , , , cord-341342-kyavg4vu 203 40 thereby thereby RB cord-341342-kyavg4vu 203 41 extending extend VBG cord-341342-kyavg4vu 203 42 the the DT cord-341342-kyavg4vu 203 43 prior prior JJ cord-341342-kyavg4vu 203 44 result result NN cord-341342-kyavg4vu 203 45 obtained obtain VBN cord-341342-kyavg4vu 203 46 with with IN cord-341342-kyavg4vu 203 47 the the DT cord-341342-kyavg4vu 203 48 N N NNP cord-341342-kyavg4vu 203 49 / / SYM cord-341342-kyavg4vu 203 50 fus2 fus2 NNP cord-341342-kyavg4vu 203 51 mutant mutant NN cord-341342-kyavg4vu 203 52 . . . cord-341342-kyavg4vu 204 1 The the DT cord-341342-kyavg4vu 204 2 N n CD cord-341342-kyavg4vu 204 3 mutants mutant NNS cord-341342-kyavg4vu 204 4 containing contain VBG cord-341342-kyavg4vu 204 5 both both DT cord-341342-kyavg4vu 204 6 amino amino NNS cord-341342-kyavg4vu 204 7 - - HYPH cord-341342-kyavg4vu 204 8 and and CC cord-341342-kyavg4vu 204 9 carboxy carboxy JJ cord-341342-kyavg4vu 204 10 - - HYPH cord-341342-kyavg4vu 204 11 terminal terminal NN cord-341342-kyavg4vu 204 12 deletions deletion NNS cord-341342-kyavg4vu 204 13 , , , cord-341342-kyavg4vu 204 14 N n CD cord-341342-kyavg4vu 204 15 / / SYM cord-341342-kyavg4vu 204 16 fus2 fus2 NNP cord-341342-kyavg4vu 204 17 / / SYM cord-341342-kyavg4vu 204 18 Acc Acc NNP cord-341342-kyavg4vu 204 19 and and CC cord-341342-kyavg4vu 204 20 N N NNP cord-341342-kyavg4vu 204 21 / / SYM cord-341342-kyavg4vu 204 22 fus4 fus4 NNP cord-341342-kyavg4vu 204 23 / / SYM cord-341342-kyavg4vu 204 24 Acc Acc NNP cord-341342-kyavg4vu 204 25 , , , cord-341342-kyavg4vu 204 26 were be VBD cord-341342-kyavg4vu 204 27 at at RB cord-341342-kyavg4vu 204 28 least least RBS cord-341342-kyavg4vu 204 29 60 60 CD cord-341342-kyavg4vu 204 30 % % NN cord-341342-kyavg4vu 204 31 as as RB cord-341342-kyavg4vu 204 32 able able JJ cord-341342-kyavg4vu 204 33 as as IN cord-341342-kyavg4vu 204 34 N n CD cord-341342-kyavg4vu 204 35 / / SYM cord-341342-kyavg4vu 204 36 fusl fusl NNS cord-341342-kyavg4vu 204 37 ( ( -LRB- cord-341342-kyavg4vu 204 38 or or CC cord-341342-kyavg4vu 204 39 at at RB cord-341342-kyavg4vu 204 40 least least RBS cord-341342-kyavg4vu 204 41 40 40 CD cord-341342-kyavg4vu 204 42 % % NN cord-341342-kyavg4vu 204 43 as as RB cord-341342-kyavg4vu 204 44 able able JJ cord-341342-kyavg4vu 204 45 as as IN cord-341342-kyavg4vu 204 46 full full JJ cord-341342-kyavg4vu 204 47 - - HYPH cord-341342-kyavg4vu 204 48 length length NN cord-341342-kyavg4vu 204 49 N n CD cord-341342-kyavg4vu 204 50 ) ) -RRB- cord-341342-kyavg4vu 204 51 to to TO cord-341342-kyavg4vu 204 52 bind bind VB cord-341342-kyavg4vu 204 53 to to IN cord-341342-kyavg4vu 204 54 RNA RNA NNP cord-341342-kyavg4vu 204 55 ( ( -LRB- cord-341342-kyavg4vu 204 56 Table Table NNP cord-341342-kyavg4vu 204 57 1 1 CD cord-341342-kyavg4vu 204 58 ) ) -RRB- cord-341342-kyavg4vu 204 59 . . . cord-341342-kyavg4vu 205 1 Therefore therefore RB cord-341342-kyavg4vu 205 2 , , , cord-341342-kyavg4vu 205 3 the the DT cord-341342-kyavg4vu 205 4 RNA RNA NNP cord-341342-kyavg4vu 205 5 - - HYPH cord-341342-kyavg4vu 205 6 binding bind VBG cord-341342-kyavg4vu 205 7 domain domain NN cord-341342-kyavg4vu 205 8 of of IN cord-341342-kyavg4vu 205 9 the the DT cord-341342-kyavg4vu 205 10 MHV MHV NNP cord-341342-kyavg4vu 205 11 N N NNP cord-341342-kyavg4vu 205 12 protein protein NN cord-341342-kyavg4vu 205 13 could could MD cord-341342-kyavg4vu 205 14 be be VB cord-341342-kyavg4vu 205 15 localized localize VBN cord-341342-kyavg4vu 205 16 to to IN cord-341342-kyavg4vu 205 17 a a DT cord-341342-kyavg4vu 205 18 portion portion NN cord-341342-kyavg4vu 205 19 of of IN cord-341342-kyavg4vu 205 20 the the DT cord-341342-kyavg4vu 205 21 molecule molecule NN cord-341342-kyavg4vu 205 22 encompassing encompass VBG cord-341342-kyavg4vu 205 23 amino amino NN cord-341342-kyavg4vu 205 24 acids acid NNS cord-341342-kyavg4vu 205 25 136 136 CD cord-341342-kyavg4vu 205 26 to to IN cord-341342-kyavg4vu 205 27 397 397 CD cord-341342-kyavg4vu 205 28 . . . cord-341342-kyavg4vu 206 1 The the DT cord-341342-kyavg4vu 206 2 purpose purpose NN cord-341342-kyavg4vu 206 3 of of IN cord-341342-kyavg4vu 206 4 this this DT cord-341342-kyavg4vu 206 5 study study NN cord-341342-kyavg4vu 206 6 was be VBD cord-341342-kyavg4vu 206 7 to to TO cord-341342-kyavg4vu 206 8 learn learn VB cord-341342-kyavg4vu 206 9 what what WP cord-341342-kyavg4vu 206 10 regions region NNS cord-341342-kyavg4vu 206 11 of of IN cord-341342-kyavg4vu 206 12 the the DT cord-341342-kyavg4vu 206 13 MHV MHV NNP cord-341342-kyavg4vu 206 14 N N NNP cord-341342-kyavg4vu 206 15 molecule molecule NN cord-341342-kyavg4vu 206 16 participate participate NN cord-341342-kyavg4vu 206 17 in in IN cord-341342-kyavg4vu 206 18 the the DT cord-341342-kyavg4vu 206 19 most most RBS cord-341342-kyavg4vu 206 20 salient salient JJ cord-341342-kyavg4vu 206 21 function function NN cord-341342-kyavg4vu 206 22 of of IN cord-341342-kyavg4vu 206 23 N n CD cord-341342-kyavg4vu 206 24 , , , cord-341342-kyavg4vu 206 25 its -PRON- PRP$ cord-341342-kyavg4vu 206 26 binding binding NN cord-341342-kyavg4vu 206 27 to to IN cord-341342-kyavg4vu 206 28 RNA RNA NNP cord-341342-kyavg4vu 206 29 . . . cord-341342-kyavg4vu 207 1 The the DT cord-341342-kyavg4vu 207 2 extent extent NN cord-341342-kyavg4vu 207 3 of of IN cord-341342-kyavg4vu 207 4 the the DT cord-341342-kyavg4vu 207 5 deletions deletion NNS cord-341342-kyavg4vu 207 6 and and CC cord-341342-kyavg4vu 207 7 the the DT cord-341342-kyavg4vu 207 8 RNA RNA NNP cord-341342-kyavg4vu 207 9 - - HYPH cord-341342-kyavg4vu 207 10 binding bind VBG cord-341342-kyavg4vu 207 11 competence competence NN cord-341342-kyavg4vu 207 12 of of IN cord-341342-kyavg4vu 207 13 the the DT cord-341342-kyavg4vu 207 14 various various JJ cord-341342-kyavg4vu 207 15 MHV MHV NNP cord-341342-kyavg4vu 207 16 N N NNP cord-341342-kyavg4vu 207 17 protein protein NN cord-341342-kyavg4vu 207 18 mutants mutant NNS cord-341342-kyavg4vu 207 19 constructed construct VBN cord-341342-kyavg4vu 207 20 for for IN cord-341342-kyavg4vu 207 21 this this DT cord-341342-kyavg4vu 207 22 study study NN cord-341342-kyavg4vu 207 23 are be VBP cord-341342-kyavg4vu 207 24 summarized summarize VBN cord-341342-kyavg4vu 207 25 schematically schematically RB cord-341342-kyavg4vu 207 26 in in IN cord-341342-kyavg4vu 207 27 Fig Fig NNP cord-341342-kyavg4vu 207 28 . . . cord-341342-kyavg4vu 208 1 8 8 CD cord-341342-kyavg4vu 208 2 . . . cord-341342-kyavg4vu 209 1 The the DT cord-341342-kyavg4vu 209 2 results result NNS cord-341342-kyavg4vu 209 3 indicate indicate VBP cord-341342-kyavg4vu 209 4 that that IN cord-341342-kyavg4vu 209 5 the the DT cord-341342-kyavg4vu 209 6 RNA RNA NNP cord-341342-kyavg4vu 209 7 - - HYPH cord-341342-kyavg4vu 209 8 binding bind VBG cord-341342-kyavg4vu 209 9 ability ability NN cord-341342-kyavg4vu 209 10 of of IN cord-341342-kyavg4vu 209 11 the the DT cord-341342-kyavg4vu 209 12 N n CD cord-341342-kyavg4vu 209 13 molecule molecule NN cord-341342-kyavg4vu 209 14 can can MD cord-341342-kyavg4vu 209 15 be be VB cord-341342-kyavg4vu 209 16 localized localize VBN cord-341342-kyavg4vu 209 17 to to IN cord-341342-kyavg4vu 209 18 an an DT cord-341342-kyavg4vu 209 19 internal internal JJ cord-341342-kyavg4vu 209 20 region region NN cord-341342-kyavg4vu 209 21 comprising comprise VBG cord-341342-kyavg4vu 209 22 some some DT cord-341342-kyavg4vu 209 23 262 262 CD cord-341342-kyavg4vu 209 24 amino amino NN cord-341342-kyavg4vu 209 25 acids acid NNS cord-341342-kyavg4vu 209 26 . . . cord-341342-kyavg4vu 210 1 Deletion deletion NN cord-341342-kyavg4vu 210 2 of of IN cord-341342-kyavg4vu 210 3 either either DT cord-341342-kyavg4vu 210 4 135 135 CD cord-341342-kyavg4vu 210 5 amino amino NN cord-341342-kyavg4vu 210 6 acids acid NNS cord-341342-kyavg4vu 210 7 from from IN cord-341342-kyavg4vu 210 8 the the DT cord-341342-kyavg4vu 210 9 amino amino NN cord-341342-kyavg4vu 210 10 terminus terminus NN cord-341342-kyavg4vu 210 11 or or CC cord-341342-kyavg4vu 210 12 57 57 CD cord-341342-kyavg4vu 210 13 amino amino JJ cord-341342-kyavg4vu 210 14 acids acid NNS cord-341342-kyavg4vu 210 15 from from IN cord-341342-kyavg4vu 210 16 the the DT cord-341342-kyavg4vu 210 17 carboxy carboxy JJ cord-341342-kyavg4vu 210 18 terminus terminus NN cord-341342-kyavg4vu 210 19 of of IN cord-341342-kyavg4vu 210 20 N N NNP cord-341342-kyavg4vu 210 21 produced produce VBD cord-341342-kyavg4vu 210 22 only only RB cord-341342-kyavg4vu 210 23 a a DT cord-341342-kyavg4vu 210 24 limited limited JJ cord-341342-kyavg4vu 210 25 effect effect NN cord-341342-kyavg4vu 210 26 on on IN cord-341342-kyavg4vu 210 27 the the DT cord-341342-kyavg4vu 210 28 RNA rna NN cord-341342-kyavg4vu 210 29 - - HYPH cord-341342-kyavg4vu 210 30 binding bind VBG cord-341342-kyavg4vu 210 31 function function NN cord-341342-kyavg4vu 210 32 of of IN cord-341342-kyavg4vu 210 33 this this DT cord-341342-kyavg4vu 210 34 protein protein NN cord-341342-kyavg4vu 210 35 . . . cord-341342-kyavg4vu 211 1 Moreover moreover RB cord-341342-kyavg4vu 211 2 , , , cord-341342-kyavg4vu 211 3 mutants mutant NNS cord-341342-kyavg4vu 211 4 lacking lack VBG cord-341342-kyavg4vu 211 5 both both DT cord-341342-kyavg4vu 211 6 termini terminus NNS cord-341342-kyavg4vu 211 7 still still RB cord-341342-kyavg4vu 211 8 retained retain VBD cord-341342-kyavg4vu 211 9 Fig Fig NNP cord-341342-kyavg4vu 211 10 . . NNP cord-341342-kyavg4vu 211 11 7 7 CD cord-341342-kyavg4vu 211 12 . . . cord-341342-kyavg4vu 212 1 Effect effect NN cord-341342-kyavg4vu 212 2 of of IN cord-341342-kyavg4vu 212 3 combined combine VBN cord-341342-kyavg4vu 212 4 amino amino NN cord-341342-kyavg4vu 212 5 - - HYPH cord-341342-kyavg4vu 212 6 terminal terminal NN cord-341342-kyavg4vu 212 7 and and CC cord-341342-kyavg4vu 212 8 carboxy carboxy JJ cord-341342-kyavg4vu 212 9 - - HYPH cord-341342-kyavg4vu 212 10 terminal terminal NN cord-341342-kyavg4vu 212 11 deletions deletion NNS cord-341342-kyavg4vu 212 12 on on IN cord-341342-kyavg4vu 212 13 the the DT cord-341342-kyavg4vu 212 14 ability ability NN cord-341342-kyavg4vu 212 15 of of IN cord-341342-kyavg4vu 212 16 N n CD cord-341342-kyavg4vu 212 17 protein protein NN cord-341342-kyavg4vu 212 18 to to TO cord-341342-kyavg4vu 212 19 bind bind VB cord-341342-kyavg4vu 212 20 to to IN cord-341342-kyavg4vu 212 21 RNA RNA NNP cord-341342-kyavg4vu 212 22 . . . cord-341342-kyavg4vu 213 1 In in IN cord-341342-kyavg4vu 213 2 vitro vitro FW cord-341342-kyavg4vu 213 3 - - HYPH cord-341342-kyavg4vu 213 4 synthesized synthesize VBN cord-341342-kyavg4vu 213 5 mRNAs mrnas WDT cord-341342-kyavg4vu 213 6 encoding encode VBG cord-341342-kyavg4vu 213 7 full full JJ cord-341342-kyavg4vu 213 8 - - HYPH cord-341342-kyavg4vu 213 9 length length NN cord-341342-kyavg4vu 213 10 N n CD cord-341342-kyavg4vu 213 11 protein protein NN cord-341342-kyavg4vu 213 12 , , , cord-341342-kyavg4vu 213 13 a a DT cord-341342-kyavg4vu 213 14 VSV VSV NNP cord-341342-kyavg4vu 213 15 - - HYPH cord-341342-kyavg4vu 213 16 N N NNP cord-341342-kyavg4vu 213 17 : : : cord-341342-kyavg4vu 213 18 MHV MHV NNP cord-341342-kyavg4vu 213 19 - - HYPH cord-341342-kyavg4vu 213 20 N N NNP cord-341342-kyavg4vu 213 21 fusion fusion NN cord-341342-kyavg4vu 213 22 protein protein NN cord-341342-kyavg4vu 213 23 ( ( -LRB- cord-341342-kyavg4vu 213 24 N n NN cord-341342-kyavg4vu 213 25 / / SYM cord-341342-kyavg4vu 213 26 fusl fusl NNS cord-341342-kyavg4vu 213 27 ) ) -RRB- cord-341342-kyavg4vu 213 28 , , , cord-341342-kyavg4vu 213 29 amino amino NN cord-341342-kyavg4vu 213 30 - - HYPH cord-341342-kyavg4vu 213 31 terminal terminal NN cord-341342-kyavg4vu 213 32 deletion deletion NN cord-341342-kyavg4vu 213 33 mutants mutant NNS cord-341342-kyavg4vu 213 34 ( ( -LRB- cord-341342-kyavg4vu 213 35 N N NNP cord-341342-kyavg4vu 213 36 / / SYM cord-341342-kyavg4vu 213 37 fus2 fus2 NNP cord-341342-kyavg4vu 213 38 and and CC cord-341342-kyavg4vu 213 39 N N NNP cord-341342-kyavg4vu 213 40 / / SYM cord-341342-kyavg4vu 213 41 fus4 fus4 NNP cord-341342-kyavg4vu 213 42 ) ) -RRB- cord-341342-kyavg4vu 213 43 , , , cord-341342-kyavg4vu 213 44 carboxy carboxy JJ cord-341342-kyavg4vu 213 45 - - HYPH cord-341342-kyavg4vu 213 46 terminal terminal NN cord-341342-kyavg4vu 213 47 deletion deletion NN cord-341342-kyavg4vu 213 48 mutants mutant NNS cord-341342-kyavg4vu 213 49 ( ( -LRB- cord-341342-kyavg4vu 213 50 N N NNP cord-341342-kyavg4vu 213 51 / / SYM cord-341342-kyavg4vu 213 52 Acc Acc NNP cord-341342-kyavg4vu 213 53 and and CC cord-341342-kyavg4vu 213 54 N N NNP cord-341342-kyavg4vu 213 55 / / SYM cord-341342-kyavg4vu 213 56 fusl fusl NNS cord-341342-kyavg4vu 213 57 / / SYM cord-341342-kyavg4vu 213 58 Acc Acc NNP cord-341342-kyavg4vu 213 59 ) ) -RRB- cord-341342-kyavg4vu 213 60 or or CC cord-341342-kyavg4vu 213 61 combined combine VBN cord-341342-kyavg4vu 213 62 aminoterminal aminoterminal NN cord-341342-kyavg4vu 213 63 and and CC cord-341342-kyavg4vu 213 64 carboxy carboxy JJ cord-341342-kyavg4vu 213 65 - - HYPH cord-341342-kyavg4vu 213 66 terminal terminal NN cord-341342-kyavg4vu 213 67 deletion deletion NN cord-341342-kyavg4vu 213 68 mutants mutant NNS cord-341342-kyavg4vu 213 69 ( ( -LRB- cord-341342-kyavg4vu 213 70 N N NNP cord-341342-kyavg4vu 213 71 / / SYM cord-341342-kyavg4vu 213 72 fus2 fus2 NNP cord-341342-kyavg4vu 213 73 / / SYM cord-341342-kyavg4vu 213 74 Acc Acc NNP cord-341342-kyavg4vu 213 75 and and CC cord-341342-kyavg4vu 213 76 N N NNP cord-341342-kyavg4vu 213 77 / / SYM cord-341342-kyavg4vu 213 78 fus4 fus4 NNP cord-341342-kyavg4vu 213 79 / / SYM cord-341342-kyavg4vu 213 80 Acc Acc NNP cord-341342-kyavg4vu 213 81 ) ) -RRB- cord-341342-kyavg4vu 213 82 were be VBD cord-341342-kyavg4vu 213 83 translated translate VBN cord-341342-kyavg4vu 213 84 and and CC cord-341342-kyavg4vu 213 85 subsequently subsequently RB cord-341342-kyavg4vu 213 86 incubated incubate VBD cord-341342-kyavg4vu 213 87 for for IN cord-341342-kyavg4vu 213 88 30 30 CD cord-341342-kyavg4vu 213 89 min min NN cord-341342-kyavg4vu 213 90 at at IN cord-341342-kyavg4vu 213 91 30 30 CD cord-341342-kyavg4vu 213 92 ° ° NN cord-341342-kyavg4vu 213 93 C c NN cord-341342-kyavg4vu 213 94 with with IN cord-341342-kyavg4vu 213 95 either either CC cord-341342-kyavg4vu 213 96 H20 H20 NNP cord-341342-kyavg4vu 213 97 or or CC cord-341342-kyavg4vu 213 98 with with IN cord-341342-kyavg4vu 213 99 30 30 CD cord-341342-kyavg4vu 213 100 gg/ gg/ NNS cord-341342-kyavg4vu 214 1 ml ml LS cord-341342-kyavg4vu 214 2 RNase RNase NNP cord-341342-kyavg4vu 214 3 A. A. NNP cord-341342-kyavg4vu 214 4 Control Control NNP cord-341342-kyavg4vu 214 5 reactions reaction NNS cord-341342-kyavg4vu 214 6 contained contain VBD cord-341342-kyavg4vu 214 7 no no DT cord-341342-kyavg4vu 214 8 exogenous exogenous JJ cord-341342-kyavg4vu 214 9 mRNA mrna NN cord-341342-kyavg4vu 214 10 ( ( -LRB- cord-341342-kyavg4vu 214 11 a a DT cord-341342-kyavg4vu 214 12 , , , cord-341342-kyavg4vu 214 13 b b NN cord-341342-kyavg4vu 214 14 , , , cord-341342-kyavg4vu 214 15 a a DT cord-341342-kyavg4vu 214 16 ' ' '' cord-341342-kyavg4vu 214 17 and and CC cord-341342-kyavg4vu 214 18 b b NNP cord-341342-kyavg4vu 214 19 ' ' '' cord-341342-kyavg4vu 214 20 ) ) -RRB- cord-341342-kyavg4vu 214 21 . . . cord-341342-kyavg4vu 215 1 [ [ -LRB- cord-341342-kyavg4vu 215 2 35S]methionine 35s]methionine CD cord-341342-kyavg4vu 215 3 - - HYPH cord-341342-kyavg4vu 215 4 labeled label VBN cord-341342-kyavg4vu 215 5 samples sample NNS cord-341342-kyavg4vu 215 6 were be VBD cord-341342-kyavg4vu 215 7 analyzed analyze VBN cord-341342-kyavg4vu 215 8 by by IN cord-341342-kyavg4vu 215 9 SDS SDS NNP cord-341342-kyavg4vu 215 10 - - HYPH cord-341342-kyavg4vu 215 11 PAGE PAGE NNP cord-341342-kyavg4vu 215 12 ( ( -LRB- cord-341342-kyavg4vu 215 13 a a FW cord-341342-kyavg4vu 215 14 - - HYPH cord-341342-kyavg4vu 215 15 r r NN cord-341342-kyavg4vu 215 16 ) ) -RRB- cord-341342-kyavg4vu 215 17 and and CC cord-341342-kyavg4vu 215 18 by by IN cord-341342-kyavg4vu 215 19 nondenaturing nondenature VBG cord-341342-kyavg4vu 215 20 ( ( -LRB- cord-341342-kyavg4vu 215 21 ND)-PAGE ND)-PAGE NNP cord-341342-kyavg4vu 215 22 ( ( -LRB- cord-341342-kyavg4vu 215 23 a'-r a'-r NNP cord-341342-kyavg4vu 215 24 ' ' '' cord-341342-kyavg4vu 215 25 ) ) -RRB- cord-341342-kyavg4vu 216 1 RNA rna NN cord-341342-kyavg4vu 216 2 - - HYPH cord-341342-kyavg4vu 216 3 binding bind VBG cord-341342-kyavg4vu 216 4 ability ability NN cord-341342-kyavg4vu 216 5 . . . cord-341342-kyavg4vu 217 1 By by IN cord-341342-kyavg4vu 217 2 contrast contrast NN cord-341342-kyavg4vu 217 3 , , , cord-341342-kyavg4vu 217 4 deletions deletion NNS cord-341342-kyavg4vu 217 5 occurring occur VBG cord-341342-kyavg4vu 217 6 within within IN cord-341342-kyavg4vu 217 7 the the DT cord-341342-kyavg4vu 217 8 internal internal JJ cord-341342-kyavg4vu 217 9 region region NN cord-341342-kyavg4vu 217 10 or or CC cord-341342-kyavg4vu 217 11 entering enter VBG cord-341342-kyavg4vu 217 12 further far RBR cord-341342-kyavg4vu 217 13 into into IN cord-341342-kyavg4vu 217 14 it -PRON- PRP cord-341342-kyavg4vu 217 15 via via IN cord-341342-kyavg4vu 217 16 the the DT cord-341342-kyavg4vu 217 17 carboxy carboxy JJ cord-341342-kyavg4vu 217 18 terminus terminus NN cord-341342-kyavg4vu 217 19 of of IN cord-341342-kyavg4vu 217 20 the the DT cord-341342-kyavg4vu 217 21 molecule molecule NN cord-341342-kyavg4vu 217 22 effectively effectively RB cord-341342-kyavg4vu 217 23 abolished abolish VBD cord-341342-kyavg4vu 217 24 R R NNP cord-341342-kyavg4vu 217 25 N N NNP cord-341342-kyavg4vu 217 26 A A NNP cord-341342-kyavg4vu 217 27 binding binding NN cord-341342-kyavg4vu 217 28 . . . cord-341342-kyavg4vu 218 1 The the DT cord-341342-kyavg4vu 218 2 regions region NNS cord-341342-kyavg4vu 218 3 of of IN cord-341342-kyavg4vu 218 4 N n CD cord-341342-kyavg4vu 218 5 defined define VBN cord-341342-kyavg4vu 218 6 by by IN cord-341342-kyavg4vu 218 7 this this DT cord-341342-kyavg4vu 218 8 analysis analysis NN cord-341342-kyavg4vu 218 9 coincide coincide VBP cord-341342-kyavg4vu 218 10 strikingly strikingly RB cord-341342-kyavg4vu 218 11 with with IN cord-341342-kyavg4vu 218 12 those those DT cord-341342-kyavg4vu 218 13 of of IN cord-341342-kyavg4vu 218 14 a a DT cord-341342-kyavg4vu 218 15 three three CD cord-341342-kyavg4vu 218 16 domain domain NN cord-341342-kyavg4vu 218 17 model model NN cord-341342-kyavg4vu 218 18 for for IN cord-341342-kyavg4vu 218 19 the the DT cord-341342-kyavg4vu 218 20 molecule molecule NN cord-341342-kyavg4vu 218 21 that that WDT cord-341342-kyavg4vu 218 22 we -PRON- PRP cord-341342-kyavg4vu 218 23 have have VBP cord-341342-kyavg4vu 218 24 proposed propose VBN cord-341342-kyavg4vu 218 25 previously previously RB cord-341342-kyavg4vu 218 26 on on IN cord-341342-kyavg4vu 218 27 the the DT cord-341342-kyavg4vu 218 28 basis basis NN cord-341342-kyavg4vu 218 29 of of IN cord-341342-kyavg4vu 218 30 a a DT cord-341342-kyavg4vu 218 31 comparison comparison NN cord-341342-kyavg4vu 218 32 of of IN cord-341342-kyavg4vu 218 33 the the DT cord-341342-kyavg4vu 218 34 sequences sequence NNS cord-341342-kyavg4vu 218 35 of of IN cord-341342-kyavg4vu 218 36 the the DT cord-341342-kyavg4vu 218 37 N n CD cord-341342-kyavg4vu 218 38 proteins protein NNS cord-341342-kyavg4vu 218 39 of of IN cord-341342-kyavg4vu 218 40 five five CD cord-341342-kyavg4vu 218 41 different different JJ cord-341342-kyavg4vu 218 42 strains strain NNS cord-341342-kyavg4vu 218 43 of of IN cord-341342-kyavg4vu 218 44 MHV MHV NNP cord-341342-kyavg4vu 218 45 [ [ -LRB- cord-341342-kyavg4vu 218 46 20 20 CD cord-341342-kyavg4vu 218 47 ] ] -RRB- cord-341342-kyavg4vu 218 48 . . . cord-341342-kyavg4vu 219 1 The the DT cord-341342-kyavg4vu 219 2 present present JJ cord-341342-kyavg4vu 219 3 work work NN cord-341342-kyavg4vu 219 4 suggests suggest VBZ cord-341342-kyavg4vu 219 5 that that IN cord-341342-kyavg4vu 219 6 these these DT cord-341342-kyavg4vu 219 7 three three CD cord-341342-kyavg4vu 219 8 domains domain NNS cord-341342-kyavg4vu 219 9 of of IN cord-341342-kyavg4vu 219 10 N n CD cord-341342-kyavg4vu 219 11 are be VBP cord-341342-kyavg4vu 219 12 not not RB cord-341342-kyavg4vu 219 13 only only RB cord-341342-kyavg4vu 219 14 structurally structurally RB cord-341342-kyavg4vu 219 15 distinct distinct JJ cord-341342-kyavg4vu 219 16 but but CC cord-341342-kyavg4vu 219 17 are be VBP cord-341342-kyavg4vu 219 18 functionally functionally RB cord-341342-kyavg4vu 219 19 separable separable JJ cord-341342-kyavg4vu 219 20 as as RB cord-341342-kyavg4vu 219 21 well well RB cord-341342-kyavg4vu 219 22 . . . cord-341342-kyavg4vu 220 1 The the DT cord-341342-kyavg4vu 220 2 RNA RNA NNP cord-341342-kyavg4vu 220 3 - - HYPH cord-341342-kyavg4vu 220 4 binding bind VBG cord-341342-kyavg4vu 220 5 characteristic characteristic NN cord-341342-kyavg4vu 220 6 of of IN cord-341342-kyavg4vu 220 7 the the DT cord-341342-kyavg4vu 220 8 N N NNP cord-341342-kyavg4vu 220 9 molecule molecule NN cord-341342-kyavg4vu 220 10 resides reside VBZ cord-341342-kyavg4vu 220 11 within within IN cord-341342-kyavg4vu 220 12 domain domain NN cord-341342-kyavg4vu 220 13 II II NNP cord-341342-kyavg4vu 220 14 , , , cord-341342-kyavg4vu 220 15 whereas whereas IN cord-341342-kyavg4vu 220 16 domains domain NNS cord-341342-kyavg4vu 220 17 I -PRON- PRP cord-341342-kyavg4vu 220 18 and and CC cord-341342-kyavg4vu 220 19 III III NNP cord-341342-kyavg4vu 220 20 are be VBP cord-341342-kyavg4vu 220 21 expendable expendable JJ cord-341342-kyavg4vu 220 22 with with IN cord-341342-kyavg4vu 220 23 respect respect NN cord-341342-kyavg4vu 220 24 to to IN cord-341342-kyavg4vu 220 25 this this DT cord-341342-kyavg4vu 220 26 activity activity NN cord-341342-kyavg4vu 220 27 . . . cord-341342-kyavg4vu 221 1 Since since IN cord-341342-kyavg4vu 221 2 the the DT cord-341342-kyavg4vu 221 3 deletions deletion NNS cord-341342-kyavg4vu 221 4 entering enter VBG cord-341342-kyavg4vu 221 5 domain domain NN cord-341342-kyavg4vu 221 6 II II NNP cord-341342-kyavg4vu 221 7 are be VBP cord-341342-kyavg4vu 221 8 mutations mutation NNS cord-341342-kyavg4vu 221 9 to to IN cord-341342-kyavg4vu 221 10 loss loss NN cord-341342-kyavg4vu 221 11 of of IN cord-341342-kyavg4vu 221 12 function function NN cord-341342-kyavg4vu 221 13 , , , cord-341342-kyavg4vu 221 14 it -PRON- PRP cord-341342-kyavg4vu 221 15 [ [ -LRB- cord-341342-kyavg4vu 221 16 6 6 CD cord-341342-kyavg4vu 221 17 ] ] -RRB- cord-341342-kyavg4vu 221 18 or or CC cord-341342-kyavg4vu 221 19 recognizable recognizable JJ cord-341342-kyavg4vu 221 20 similarity similarity NN cord-341342-kyavg4vu 221 21 to to IN cord-341342-kyavg4vu 221 22 recently recently RB cord-341342-kyavg4vu 221 23 described describe VBN cord-341342-kyavg4vu 221 24 arginine arginine NN cord-341342-kyavg4vu 221 25 - - HYPH cord-341342-kyavg4vu 221 26 rich rich JJ cord-341342-kyavg4vu 221 27 [ [ -LRB- cord-341342-kyavg4vu 221 28 9 9 CD cord-341342-kyavg4vu 221 29 ] ] -RRB- cord-341342-kyavg4vu 221 30 or or CC cord-341342-kyavg4vu 221 31 methionine methionine NN cord-341342-kyavg4vu 221 32 - - HYPH cord-341342-kyavg4vu 221 33 rich rich JJ cord-341342-kyavg4vu 221 34 [ [ -LRB- cord-341342-kyavg4vu 221 35 23 23 CD cord-341342-kyavg4vu 221 36 ] ] -RRB- cord-341342-kyavg4vu 221 37 RNA rna NN cord-341342-kyavg4vu 221 38 - - HYPH cord-341342-kyavg4vu 221 39 binding bind VBG cord-341342-kyavg4vu 221 40 domains domain NNS cord-341342-kyavg4vu 221 41 . . . cord-341342-kyavg4vu 222 1 Thus thus RB cord-341342-kyavg4vu 222 2 , , , cord-341342-kyavg4vu 222 3 the the DT cord-341342-kyavg4vu 222 4 rules rule NNS cord-341342-kyavg4vu 222 5 governing govern VBG cord-341342-kyavg4vu 222 6 this this DT cord-341342-kyavg4vu 222 7 particular particular JJ cord-341342-kyavg4vu 222 8 mode mode NN cord-341342-kyavg4vu 222 9 of of IN cord-341342-kyavg4vu 222 10 protein protein NN cord-341342-kyavg4vu 222 11 - - HYPH cord-341342-kyavg4vu 222 12 RNA rna NN cord-341342-kyavg4vu 222 13 interaction interaction NN cord-341342-kyavg4vu 222 14 remain remain VBP cord-341342-kyavg4vu 222 15 to to TO cord-341342-kyavg4vu 222 16 be be VB cord-341342-kyavg4vu 222 17 elucidated elucidate VBN cord-341342-kyavg4vu 222 18 . . . cord-341342-kyavg4vu 223 1 In in IN cord-341342-kyavg4vu 223 2 Fig Fig NNP cord-341342-kyavg4vu 223 3 . . . cord-341342-kyavg4vu 224 1 8 8 CD cord-341342-kyavg4vu 224 2 , , , cord-341342-kyavg4vu 224 3 the the DT cord-341342-kyavg4vu 224 4 RNA rna NN cord-341342-kyavg4vu 224 5 - - HYPH cord-341342-kyavg4vu 224 6 binding bind VBG cord-341342-kyavg4vu 224 7 ability ability NN cord-341342-kyavg4vu 224 8 of of IN cord-341342-kyavg4vu 224 9 N N NNP cord-341342-kyavg4vu 224 10 / / SYM cord-341342-kyavg4vu 224 11 Alb4 Alb4 NNP cord-341342-kyavg4vu 224 12 has have VBZ cord-341342-kyavg4vu 224 13 been be VBN cord-341342-kyavg4vu 224 14 scored score VBN cord-341342-kyavg4vu 224 15 as as RB cord-341342-kyavg4vu 224 16 equivocal equivocal JJ cord-341342-kyavg4vu 224 17 , , , cord-341342-kyavg4vu 224 18 based base VBN cord-341342-kyavg4vu 224 19 on on IN cord-341342-kyavg4vu 224 20 our -PRON- PRP$ cord-341342-kyavg4vu 224 21 recent recent JJ cord-341342-kyavg4vu 224 22 finding finding NN cord-341342-kyavg4vu 224 23 that that IN cord-341342-kyavg4vu 224 24 the the DT cord-341342-kyavg4vu 224 25 ability ability NN cord-341342-kyavg4vu 224 26 of of IN cord-341342-kyavg4vu 224 27 this this DT cord-341342-kyavg4vu 224 28 mutant mutant JJ cord-341342-kyavg4vu 224 29 protein protein NN cord-341342-kyavg4vu 224 30 to to TO cord-341342-kyavg4vu 224 31 enter enter VB cord-341342-kyavg4vu 224 32 a a DT cord-341342-kyavg4vu 224 33 nondenaturing nondenature VBG cord-341342-kyavg4vu 224 34 gel gel NN cord-341342-kyavg4vu 224 35 is be VBZ cord-341342-kyavg4vu 224 36 temperature temperature NN cord-341342-kyavg4vu 224 37 - - HYPH cord-341342-kyavg4vu 224 38 sensitive sensitive JJ cord-341342-kyavg4vu 224 39 with with IN cord-341342-kyavg4vu 224 40 respect respect NN cord-341342-kyavg4vu 224 41 to to IN cord-341342-kyavg4vu 224 42 that that DT cord-341342-kyavg4vu 224 43 of of IN cord-341342-kyavg4vu 224 44 wild wild JJ cord-341342-kyavg4vu 224 45 type type NN cord-341342-kyavg4vu 224 46 N n NN cord-341342-kyavg4vu 224 47 ( ( -LRB- cord-341342-kyavg4vu 224 48 Koetzner Koetzner NNP cord-341342-kyavg4vu 224 49 and and CC cord-341342-kyavg4vu 224 50 Masters Masters NNPS cord-341342-kyavg4vu 224 51 , , , cord-341342-kyavg4vu 224 52 unpubl unpubl NNS cord-341342-kyavg4vu 224 53 , , , cord-341342-kyavg4vu 224 54 results result NNS cord-341342-kyavg4vu 224 55 ) ) -RRB- cord-341342-kyavg4vu 224 56 . . . cord-341342-kyavg4vu 225 1 In in IN cord-341342-kyavg4vu 225 2 addition addition NN cord-341342-kyavg4vu 225 3 , , , cord-341342-kyavg4vu 225 4 revertants revertant NNS cord-341342-kyavg4vu 225 5 of of IN cord-341342-kyavg4vu 225 6 the the DT cord-341342-kyavg4vu 225 7 Albany-4 Albany-4 NNP cord-341342-kyavg4vu 225 8 mutant mutant NN cord-341342-kyavg4vu 225 9 have have VBP cord-341342-kyavg4vu 225 10 been be VBN cord-341342-kyavg4vu 225 11 selected select VBN cord-341342-kyavg4vu 225 12 , , , cord-341342-kyavg4vu 225 13 and and CC cord-341342-kyavg4vu 225 14 all all DT cord-341342-kyavg4vu 225 15 of of IN cord-341342-kyavg4vu 225 16 these these DT cord-341342-kyavg4vu 225 17 have have VBP cord-341342-kyavg4vu 225 18 N n CD cord-341342-kyavg4vu 225 19 proteins protein NNS cord-341342-kyavg4vu 225 20 which which WDT cord-341342-kyavg4vu 225 21 no no RB cord-341342-kyavg4vu 225 22 longer longer RB cord-341342-kyavg4vu 225 23 exhibit exhibit VBP cord-341342-kyavg4vu 225 24 the the DT cord-341342-kyavg4vu 225 25 RNA rna NN cord-341342-kyavg4vu 225 26 - - HYPH cord-341342-kyavg4vu 225 27 binding bind VBG cord-341342-kyavg4vu 225 28 temperature temperature NN cord-341342-kyavg4vu 225 29 - - HYPH cord-341342-kyavg4vu 225 30 sensitivity sensitivity NN cord-341342-kyavg4vu 225 31 of of IN cord-341342-kyavg4vu 225 32 N N NNP cord-341342-kyavg4vu 225 33 / / SYM cord-341342-kyavg4vu 225 34 Alb4 Alb4 NNP cord-341342-kyavg4vu 225 35 . . . cord-341342-kyavg4vu 226 1 For for IN cord-341342-kyavg4vu 226 2 the the DT cord-341342-kyavg4vu 226 3 one one CD cord-341342-kyavg4vu 226 4 revertant revertant NN cord-341342-kyavg4vu 226 5 that that WDT cord-341342-kyavg4vu 226 6 we -PRON- PRP cord-341342-kyavg4vu 226 7 have have VBP cord-341342-kyavg4vu 226 8 analyzed analyze VBN cord-341342-kyavg4vu 226 9 in in IN cord-341342-kyavg4vu 226 10 detail detail NN cord-341342-kyavg4vu 226 11 , , , cord-341342-kyavg4vu 226 12 the the DT cord-341342-kyavg4vu 226 13 single single JJ cord-341342-kyavg4vu 226 14 point point NN cord-341342-kyavg4vu 226 15 mutation mutation NN cord-341342-kyavg4vu 226 16 that that WDT cord-341342-kyavg4vu 226 17 causes cause VBZ cord-341342-kyavg4vu 226 18 this this DT cord-341342-kyavg4vu 226 19 restoration restoration NN cord-341342-kyavg4vu 226 20 of of IN cord-341342-kyavg4vu 226 21 RNA rna NN cord-341342-kyavg4vu 226 22 - - HYPH cord-341342-kyavg4vu 226 23 binding bind VBG cord-341342-kyavg4vu 226 24 ability ability NN cord-341342-kyavg4vu 226 25 resides reside VBZ cord-341342-kyavg4vu 226 26 in in IN cord-341342-kyavg4vu 226 27 the the DT cord-341342-kyavg4vu 226 28 distal distal JJ cord-341342-kyavg4vu 226 29 third third NN cord-341342-kyavg4vu 226 30 of of IN cord-341342-kyavg4vu 226 31 domain domain NN cord-341342-kyavg4vu 226 32 II II NNP cord-341342-kyavg4vu 226 33 . . . cord-341342-kyavg4vu 227 1 This this DT cord-341342-kyavg4vu 227 2 finding find VBG cord-341342-kyavg4vu 227 3 further further RB cord-341342-kyavg4vu 227 4 underscores underscore VBZ cord-341342-kyavg4vu 227 5 the the DT cord-341342-kyavg4vu 227 6 role role NN cord-341342-kyavg4vu 227 7 of of IN cord-341342-kyavg4vu 227 8 domain domain NN cord-341342-kyavg4vu 227 9 II II NNP cord-341342-kyavg4vu 227 10 in in IN cord-341342-kyavg4vu 227 11 RNA RNA NNP cord-341342-kyavg4vu 227 12 binding binding NN cord-341342-kyavg4vu 227 13 , , , cord-341342-kyavg4vu 227 14 and and CC cord-341342-kyavg4vu 227 15 supports support VBZ cord-341342-kyavg4vu 227 16 the the DT cord-341342-kyavg4vu 227 17 notion notion NN cord-341342-kyavg4vu 227 18 that that IN cord-341342-kyavg4vu 227 19 the the DT cord-341342-kyavg4vu 227 20 nondenaturing nondenature VBG cord-341342-kyavg4vu 227 21 gel gel NN cord-341342-kyavg4vu 227 22 assay assay NN cord-341342-kyavg4vu 227 23 measures measure VBZ cord-341342-kyavg4vu 227 24 a a DT cord-341342-kyavg4vu 227 25 biologically biologically RB cord-341342-kyavg4vu 227 26 relevant relevant JJ cord-341342-kyavg4vu 227 27 property property NN cord-341342-kyavg4vu 227 28 of of IN cord-341342-kyavg4vu 227 29 the the DT cord-341342-kyavg4vu 227 30 N n CD cord-341342-kyavg4vu 227 31 molecule molecule NN cord-341342-kyavg4vu 227 32 . . . cord-341342-kyavg4vu 228 1 Although although IN cord-341342-kyavg4vu 228 2 the the DT cord-341342-kyavg4vu 228 3 N n CD cord-341342-kyavg4vu 228 4 protein protein NN cord-341342-kyavg4vu 228 5 may may MD cord-341342-kyavg4vu 228 6 recognize recognize VB cord-341342-kyavg4vu 228 7 a a DT cord-341342-kyavg4vu 228 8 specific specific JJ cord-341342-kyavg4vu 228 9 nucleation nucleation NN cord-341342-kyavg4vu 228 10 sequence sequence NN cord-341342-kyavg4vu 228 11 or or CC cord-341342-kyavg4vu 228 12 secondary secondary JJ cord-341342-kyavg4vu 228 13 structure structure NN cord-341342-kyavg4vu 228 14 on on IN cord-341342-kyavg4vu 228 15 the the DT cord-341342-kyavg4vu 228 16 MHV MHV NNP cord-341342-kyavg4vu 228 17 genome genome NN cord-341342-kyavg4vu 228 18 , , , cord-341342-kyavg4vu 228 19 it -PRON- PRP cord-341342-kyavg4vu 228 20 must must MD cord-341342-kyavg4vu 228 21 also also RB cord-341342-kyavg4vu 228 22 possess possess VB cord-341342-kyavg4vu 228 23 a a DT cord-341342-kyavg4vu 228 24 more more RBR cord-341342-kyavg4vu 228 25 general general JJ cord-341342-kyavg4vu 228 26 non non JJ cord-341342-kyavg4vu 228 27 - - JJ cord-341342-kyavg4vu 228 28 sequence sequence JJ cord-341342-kyavg4vu 228 29 - - HYPH cord-341342-kyavg4vu 228 30 specific specific JJ cord-341342-kyavg4vu 228 31 RNA rna NN cord-341342-kyavg4vu 228 32 - - HYPH cord-341342-kyavg4vu 228 33 binding bind VBG cord-341342-kyavg4vu 228 34 capability capability NN cord-341342-kyavg4vu 228 35 , , , cord-341342-kyavg4vu 228 36 since since IN cord-341342-kyavg4vu 228 37 it -PRON- PRP cord-341342-kyavg4vu 228 38 encapsidates encapsidate VBZ cord-341342-kyavg4vu 228 39 the the DT cord-341342-kyavg4vu 228 40 entire entire JJ cord-341342-kyavg4vu 228 41 length length NN cord-341342-kyavg4vu 228 42 of of IN cord-341342-kyavg4vu 228 43 the the DT cord-341342-kyavg4vu 228 44 31,000 31,000 CD cord-341342-kyavg4vu 228 45 nucleotide nucleotide JJ cord-341342-kyavg4vu 228 46 genome genome NN cord-341342-kyavg4vu 228 47 , , , cord-341342-kyavg4vu 228 48 which which WDT cord-341342-kyavg4vu 228 49 does do VBZ cord-341342-kyavg4vu 228 50 not not RB cord-341342-kyavg4vu 228 51 appear appear VB cord-341342-kyavg4vu 228 52 to to TO cord-341342-kyavg4vu 228 53 contain contain VB cord-341342-kyavg4vu 228 54 any any DT cord-341342-kyavg4vu 228 55 iterated iterate VBN cord-341342-kyavg4vu 228 56 pattern pattern NN cord-341342-kyavg4vu 228 57 of of IN cord-341342-kyavg4vu 228 58 potential potential JJ cord-341342-kyavg4vu 228 59 RNA RNA NNP cord-341342-kyavg4vu 228 60 recognition recognition NN cord-341342-kyavg4vu 228 61 sequences sequence NNS cord-341342-kyavg4vu 228 62 . . . cord-341342-kyavg4vu 229 1 This this DT cord-341342-kyavg4vu 229 2 general general JJ cord-341342-kyavg4vu 229 3 mode mode NN cord-341342-kyavg4vu 229 4 of of IN cord-341342-kyavg4vu 229 5 RNA RNA NNP cord-341342-kyavg4vu 229 6 binding binding NN cord-341342-kyavg4vu 229 7 may may MD cord-341342-kyavg4vu 229 8 be be VB cord-341342-kyavg4vu 229 9 a a DT cord-341342-kyavg4vu 229 10 common common JJ cord-341342-kyavg4vu 229 11 characteristic characteristic NN cord-341342-kyavg4vu 229 12 of of IN cord-341342-kyavg4vu 229 13 the the DT cord-341342-kyavg4vu 229 14 N N NNP cord-341342-kyavg4vu 229 15 ( ( -LRB- cord-341342-kyavg4vu 229 16 NP NP NNP cord-341342-kyavg4vu 229 17 ) ) -RRB- cord-341342-kyavg4vu 229 18 proteins protein NNS cord-341342-kyavg4vu 229 19 of of IN cord-341342-kyavg4vu 229 20 RNA RNA NNP cord-341342-kyavg4vu 229 21 viruses virus NNS cord-341342-kyavg4vu 229 22 having have VBG cord-341342-kyavg4vu 229 23 helical helical JJ cord-341342-kyavg4vu 229 24 nucleocapsids nucleocapsid NNS cord-341342-kyavg4vu 229 25 . . . cord-341342-kyavg4vu 230 1 Bacterially Bacterially NNP cord-341342-kyavg4vu 230 2 expressed express VBD cord-341342-kyavg4vu 230 3 NP NP NNP cord-341342-kyavg4vu 230 4 protein protein NN cord-341342-kyavg4vu 230 5 of of IN cord-341342-kyavg4vu 230 6 influenza influenza NN cord-341342-kyavg4vu 230 7 virus virus NN cord-341342-kyavg4vu 230 8 was be VBD cord-341342-kyavg4vu 230 9 shown show VBN cord-341342-kyavg4vu 230 10 to to TO cord-341342-kyavg4vu 230 11 bind bind VB cord-341342-kyavg4vu 230 12 well well RB cord-341342-kyavg4vu 230 13 to to IN cord-341342-kyavg4vu 230 14 both both CC cord-341342-kyavg4vu 230 15 viral viral JJ cord-341342-kyavg4vu 230 16 and and CC cord-341342-kyavg4vu 230 17 nonviral nonviral NNP cord-341342-kyavg4vu 230 18 RNA RNA NNP cord-341342-kyavg4vu 230 19 substrates substrate NNS cord-341342-kyavg4vu 230 20 when when WRB cord-341342-kyavg4vu 230 21 assayed assay VBN cord-341342-kyavg4vu 230 22 in in IN cord-341342-kyavg4vu 230 23 vitro vitro FW cord-341342-kyavg4vu 230 24 [ [ -LRB- cord-341342-kyavg4vu 230 25 4 4 CD cord-341342-kyavg4vu 230 26 ] ] -RRB- cord-341342-kyavg4vu 230 27 . . . cord-341342-kyavg4vu 231 1 The the DT cord-341342-kyavg4vu 231 2 N N NNP cord-341342-kyavg4vu 231 3 protein protein NN cord-341342-kyavg4vu 231 4 of of IN cord-341342-kyavg4vu 231 5 VSV VSV NNP cord-341342-kyavg4vu 231 6 bound bind VBD cord-341342-kyavg4vu 231 7 tightly tightly RB cord-341342-kyavg4vu 231 8 to to IN cord-341342-kyavg4vu 231 9 cellular cellular JJ cord-341342-kyavg4vu 231 10 RNAs rna NNS cord-341342-kyavg4vu 231 11 when when WRB cord-341342-kyavg4vu 231 12 synthesized synthesize VBN cord-341342-kyavg4vu 231 13 in in IN cord-341342-kyavg4vu 231 14 the the DT cord-341342-kyavg4vu 231 15 absence absence NN cord-341342-kyavg4vu 231 16 of of IN cord-341342-kyavg4vu 231 17 other other JJ cord-341342-kyavg4vu 231 18 viral viral JJ cord-341342-kyavg4vu 231 19 proteins protein NNS cord-341342-kyavg4vu 231 20 in in IN cord-341342-kyavg4vu 231 21 an an DT cord-341342-kyavg4vu 231 22 in in IN cord-341342-kyavg4vu 231 23 vivo vivo NN cord-341342-kyavg4vu 231 24 expression expression NN cord-341342-kyavg4vu 231 25 system system NN cord-341342-kyavg4vu 231 26 [ [ -LRB- cord-341342-kyavg4vu 231 27 28 28 CD cord-341342-kyavg4vu 231 28 ] ] -RRB- cord-341342-kyavg4vu 231 29 , , , cord-341342-kyavg4vu 231 30 and and CC cord-341342-kyavg4vu 231 31 it -PRON- PRP cord-341342-kyavg4vu 231 32 bound bind VBD cord-341342-kyavg4vu 231 33 to to IN cord-341342-kyavg4vu 231 34 endogenous endogenous JJ cord-341342-kyavg4vu 231 35 reticulocyte reticulocyte NN cord-341342-kyavg4vu 231 36 RNAs rna NNS cord-341342-kyavg4vu 231 37 or or CC cord-341342-kyavg4vu 231 38 added add VBN cord-341342-kyavg4vu 231 39 RNA RNA NNP cord-341342-kyavg4vu 231 40 species specie NNS cord-341342-kyavg4vu 231 41 when when WRB cord-341342-kyavg4vu 231 42 expressed express VBN cord-341342-kyavg4vu 231 43 by by IN cord-341342-kyavg4vu 231 44 in in FW cord-341342-kyavg4vu 231 45 vitro vitro FW cord-341342-kyavg4vu 231 46 translation translation NN cord-341342-kyavg4vu 231 47 [ [ -LRB- cord-341342-kyavg4vu 231 48 16 16 CD cord-341342-kyavg4vu 231 49 ] ] -RRB- cord-341342-kyavg4vu 231 50 . . . cord-341342-kyavg4vu 232 1 The the DT cord-341342-kyavg4vu 232 2 measles measle NNS cord-341342-kyavg4vu 232 3 virus virus NN cord-341342-kyavg4vu 232 4 NP NP NNP cord-341342-kyavg4vu 232 5 protein protein NN cord-341342-kyavg4vu 232 6 , , , cord-341342-kyavg4vu 232 7 expressed express VBN cord-341342-kyavg4vu 232 8 in in IN cord-341342-kyavg4vu 232 9 vivo vivo NN cord-341342-kyavg4vu 232 10 by by IN cord-341342-kyavg4vu 232 11 a a DT cord-341342-kyavg4vu 232 12 vaccinia vaccinia NN cord-341342-kyavg4vu 232 13 virus virus NN cord-341342-kyavg4vu 232 14 recombinant recombinant NN cord-341342-kyavg4vu 232 15 , , , cord-341342-kyavg4vu 232 16 has have VBZ cord-341342-kyavg4vu 232 17 recently recently RB cord-341342-kyavg4vu 232 18 been be VBN cord-341342-kyavg4vu 232 19 shown show VBN cord-341342-kyavg4vu 232 20 to to TO cord-341342-kyavg4vu 232 21 assemble assemble VB cord-341342-kyavg4vu 232 22 into into IN cord-341342-kyavg4vu 232 23 structures structure NNS cord-341342-kyavg4vu 232 24 very very RB cord-341342-kyavg4vu 232 25 similar similar JJ cord-341342-kyavg4vu 232 26 to to IN cord-341342-kyavg4vu 232 27 authentic authentic JJ cord-341342-kyavg4vu 232 28 measles measles NN cord-341342-kyavg4vu 232 29 nucleocapsids nucleocapsid NNS cord-341342-kyavg4vu 232 30 , , , cord-341342-kyavg4vu 232 31 as as IN cord-341342-kyavg4vu 232 32 determined determine VBN cord-341342-kyavg4vu 232 33 by by IN cord-341342-kyavg4vu 232 34 both both DT cord-341342-kyavg4vu 232 35 electron electron NN cord-341342-kyavg4vu 232 36 microscopy microscopy NN cord-341342-kyavg4vu 232 37 and and CC cord-341342-kyavg4vu 232 38 equilibrium equilibrium NN cord-341342-kyavg4vu 232 39 sedimentation sedimentation NN cord-341342-kyavg4vu 232 40 on on IN cord-341342-kyavg4vu 232 41 CsC1 csc1 NN cord-341342-kyavg4vu 232 42 gradients gradient NNS cord-341342-kyavg4vu 232 43 [ [ -LRB- cord-341342-kyavg4vu 232 44 27 27 CD cord-341342-kyavg4vu 232 45 ] ] -RRB- cord-341342-kyavg4vu 232 46 . . . cord-341342-kyavg4vu 233 1 For for IN cord-341342-kyavg4vu 233 2 MHV MHV NNP cord-341342-kyavg4vu 233 3 , , , cord-341342-kyavg4vu 233 4 it -PRON- PRP cord-341342-kyavg4vu 233 5 has have VBZ cord-341342-kyavg4vu 233 6 been be VBN cord-341342-kyavg4vu 233 7 demonstrated demonstrate VBN cord-341342-kyavg4vu 233 8 previously previously RB cord-341342-kyavg4vu 233 9 that that IN cord-341342-kyavg4vu 233 10 the the DT cord-341342-kyavg4vu 233 11 N n CD cord-341342-kyavg4vu 233 12 protein protein NN cord-341342-kyavg4vu 233 13 of of IN cord-341342-kyavg4vu 233 14 this this DT cord-341342-kyavg4vu 233 15 virus virus NN cord-341342-kyavg4vu 233 16 can can MD cord-341342-kyavg4vu 233 17 bind bind VB cord-341342-kyavg4vu 233 18 to to IN cord-341342-kyavg4vu 233 19 a a DT cord-341342-kyavg4vu 233 20 variety variety NN cord-341342-kyavg4vu 233 21 of of IN cord-341342-kyavg4vu 233 22 viral viral JJ cord-341342-kyavg4vu 233 23 and and CC cord-341342-kyavg4vu 233 24 nonviral nonviral JJ cord-341342-kyavg4vu 233 25 RNA RNA NNP cord-341342-kyavg4vu 233 26 species specie NNS cord-341342-kyavg4vu 233 27 following follow VBG cord-341342-kyavg4vu 233 28 separation separation NN cord-341342-kyavg4vu 233 29 of of IN cord-341342-kyavg4vu 233 30 N n CD cord-341342-kyavg4vu 233 31 by by IN cord-341342-kyavg4vu 233 32 SDS SDS NNP cord-341342-kyavg4vu 233 33 - - HYPH cord-341342-kyavg4vu 233 34 PAGE PAGE NNP cord-341342-kyavg4vu 233 35 and and CC cord-341342-kyavg4vu 233 36 blotting blot VBG cord-341342-kyavg4vu 233 37 onto onto IN cord-341342-kyavg4vu 233 38 nitrocellulose nitrocellulose NN cord-341342-kyavg4vu 233 39 [ [ -LRB- cord-341342-kyavg4vu 233 40 22 22 CD cord-341342-kyavg4vu 233 41 , , , cord-341342-kyavg4vu 233 42 29 29 CD cord-341342-kyavg4vu 233 43 ] ] -RRB- cord-341342-kyavg4vu 233 44 . . . cord-341342-kyavg4vu 234 1 Thus thus RB cord-341342-kyavg4vu 234 2 , , , cord-341342-kyavg4vu 234 3 it -PRON- PRP cord-341342-kyavg4vu 234 4 is be VBZ cord-341342-kyavg4vu 234 5 not not RB cord-341342-kyavg4vu 234 6 unexpected unexpected JJ cord-341342-kyavg4vu 234 7 that that IN cord-341342-kyavg4vu 234 8 the the DT cord-341342-kyavg4vu 234 9 MHV MHV NNP cord-341342-kyavg4vu 234 10 N N NNP cord-341342-kyavg4vu 234 11 protein protein NN cord-341342-kyavg4vu 234 12 should should MD cord-341342-kyavg4vu 234 13 bind bind VB cord-341342-kyavg4vu 234 14 in in IN cord-341342-kyavg4vu 234 15 vitro vitro FW cord-341342-kyavg4vu 234 16 to to IN cord-341342-kyavg4vu 234 17 nonviral nonviral NNP cord-341342-kyavg4vu 234 18 RNA RNA NNP cord-341342-kyavg4vu 234 19 species specie NNS cord-341342-kyavg4vu 234 20 in in IN cord-341342-kyavg4vu 234 21 the the DT cord-341342-kyavg4vu 234 22 nondenaturing nondenature VBG cord-341342-kyavg4vu 234 23 gel gel NN cord-341342-kyavg4vu 234 24 assay assay NN cord-341342-kyavg4vu 234 25 reported report VBN cord-341342-kyavg4vu 234 26 here here RB cord-341342-kyavg4vu 234 27 . . . cord-341342-kyavg4vu 235 1 It -PRON- PRP cord-341342-kyavg4vu 235 2 should should MD cord-341342-kyavg4vu 235 3 be be VB cord-341342-kyavg4vu 235 4 noted note VBN cord-341342-kyavg4vu 235 5 that that IN cord-341342-kyavg4vu 235 6 an an DT cord-341342-kyavg4vu 235 7 alternative alternative JJ cord-341342-kyavg4vu 235 8 interpretation interpretation NN cord-341342-kyavg4vu 235 9 of of IN cord-341342-kyavg4vu 235 10 the the DT cord-341342-kyavg4vu 235 11 nondenaturing nondenature VBG cord-341342-kyavg4vu 235 12 gel gel NN cord-341342-kyavg4vu 235 13 assay assay NN cord-341342-kyavg4vu 235 14 is be VBZ cord-341342-kyavg4vu 235 15 that that IN cord-341342-kyavg4vu 235 16 the the DT cord-341342-kyavg4vu 235 17 MHV MHV NNP cord-341342-kyavg4vu 235 18 N N NNP cord-341342-kyavg4vu 235 19 protein protein NN cord-341342-kyavg4vu 235 20 was be VBD cord-341342-kyavg4vu 235 21 not not RB cord-341342-kyavg4vu 235 22 directly directly RB cord-341342-kyavg4vu 235 23 bound bind VBN cord-341342-kyavg4vu 235 24 to to IN cord-341342-kyavg4vu 235 25 RNA RNA NNP cord-341342-kyavg4vu 236 1 but but CC cord-341342-kyavg4vu 236 2 , , , cord-341342-kyavg4vu 236 3 rather rather RB cord-341342-kyavg4vu 236 4 , , , cord-341342-kyavg4vu 236 5 to to IN cord-341342-kyavg4vu 236 6 a a DT cord-341342-kyavg4vu 236 7 protein protein NN cord-341342-kyavg4vu 236 8 constituent constituent NN cord-341342-kyavg4vu 236 9 of of IN cord-341342-kyavg4vu 236 10 an an DT cord-341342-kyavg4vu 236 11 RNase rnase NN cord-341342-kyavg4vu 236 12 - - HYPH cord-341342-kyavg4vu 236 13 sensitive sensitive JJ cord-341342-kyavg4vu 236 14 ribonucleoprotein ribonucleoprotein NN cord-341342-kyavg4vu 236 15 in in IN cord-341342-kyavg4vu 236 16 the the DT cord-341342-kyavg4vu 236 17 reticulocyte reticulocyte NN cord-341342-kyavg4vu 236 18 lysate lysate NN cord-341342-kyavg4vu 236 19 . . . cord-341342-kyavg4vu 237 1 This this DT cord-341342-kyavg4vu 237 2 seems seem VBZ cord-341342-kyavg4vu 237 3 less less RBR cord-341342-kyavg4vu 237 4 likely likely JJ cord-341342-kyavg4vu 237 5 in in IN cord-341342-kyavg4vu 237 6 light light NN cord-341342-kyavg4vu 237 7 of of IN cord-341342-kyavg4vu 237 8 the the DT cord-341342-kyavg4vu 237 9 previously previously RB cord-341342-kyavg4vu 237 10 demonstrated demonstrate VBN cord-341342-kyavg4vu 237 11 RNA rna NN cord-341342-kyavg4vu 237 12 - - HYPH cord-341342-kyavg4vu 237 13 binding bind VBG cord-341342-kyavg4vu 237 14 activity activity NN cord-341342-kyavg4vu 237 15 of of IN cord-341342-kyavg4vu 237 16 N N NNP cord-341342-kyavg4vu 237 17 [ [ -LRB- cord-341342-kyavg4vu 237 18 22 22 CD cord-341342-kyavg4vu 237 19 , , , cord-341342-kyavg4vu 237 20 29 29 CD cord-341342-kyavg4vu 237 21 ] ] -RRB- cord-341342-kyavg4vu 237 22 , , , cord-341342-kyavg4vu 237 23 but but CC cord-341342-kyavg4vu 237 24 it -PRON- PRP cord-341342-kyavg4vu 237 25 can can MD cord-341342-kyavg4vu 237 26 not not RB cord-341342-kyavg4vu 237 27 be be VB cord-341342-kyavg4vu 237 28 ruled rule VBN cord-341342-kyavg4vu 237 29 out out RP cord-341342-kyavg4vu 237 30 until until IN cord-341342-kyavg4vu 237 31 an an DT cord-341342-kyavg4vu 237 32 MHV MHV NNP cord-341342-kyavg4vu 237 33 N N NNP cord-341342-kyavg4vu 237 34 RNA RNA NNP cord-341342-kyavg4vu 237 35 - - HYPH cord-341342-kyavg4vu 237 36 binding bind VBG cord-341342-kyavg4vu 237 37 assay assay NN cord-341342-kyavg4vu 237 38 using use VBG cord-341342-kyavg4vu 237 39 purified purify VBN cord-341342-kyavg4vu 237 40 components component NNS cord-341342-kyavg4vu 237 41 is be VBZ cord-341342-kyavg4vu 237 42 established establish VBN cord-341342-kyavg4vu 237 43 . . . cord-341342-kyavg4vu 238 1 If if IN cord-341342-kyavg4vu 238 2 such such PDT cord-341342-kyavg4vu 238 3 an an DT cord-341342-kyavg4vu 238 4 N n CD cord-341342-kyavg4vu 238 5 protein protein NN cord-341342-kyavg4vu 238 6 - - HYPH cord-341342-kyavg4vu 238 7 ribonucteoprotein ribonucteoprotein NN cord-341342-kyavg4vu 238 8 complex complex NN cord-341342-kyavg4vu 238 9 is be VBZ cord-341342-kyavg4vu 238 10 shown show VBN cord-341342-kyavg4vu 238 11 to to TO cord-341342-kyavg4vu 238 12 occur occur VB cord-341342-kyavg4vu 238 13 , , , cord-341342-kyavg4vu 238 14 then then RB cord-341342-kyavg4vu 238 15 this this DT cord-341342-kyavg4vu 238 16 may may MD cord-341342-kyavg4vu 238 17 indicate indicate VB cord-341342-kyavg4vu 238 18 a a DT cord-341342-kyavg4vu 238 19 previously previously RB cord-341342-kyavg4vu 238 20 unappreciated unappreciated JJ cord-341342-kyavg4vu 238 21 interaction interaction NN cord-341342-kyavg4vu 238 22 between between IN cord-341342-kyavg4vu 238 23 N n CD cord-341342-kyavg4vu 238 24 protein protein NN cord-341342-kyavg4vu 238 25 and and CC cord-341342-kyavg4vu 238 26 host host NN cord-341342-kyavg4vu 238 27 cell cell NN cord-341342-kyavg4vu 238 28 components component NNS cord-341342-kyavg4vu 238 29 . . . cord-341342-kyavg4vu 239 1 The the DT cord-341342-kyavg4vu 239 2 work work NN cord-341342-kyavg4vu 239 3 presented present VBN cord-341342-kyavg4vu 239 4 here here RB cord-341342-kyavg4vu 239 5 does do VBZ cord-341342-kyavg4vu 239 6 not not RB cord-341342-kyavg4vu 239 7 address address VB cord-341342-kyavg4vu 239 8 the the DT cord-341342-kyavg4vu 239 9 problem problem NN cord-341342-kyavg4vu 239 10 of of IN cord-341342-kyavg4vu 239 11 sequence sequence NN cord-341342-kyavg4vu 239 12 - - HYPH cord-341342-kyavg4vu 239 13 specific specific JJ cord-341342-kyavg4vu 239 14 RNA RNA NNP cord-341342-kyavg4vu 239 15 recognition recognition NN cord-341342-kyavg4vu 239 16 by by IN cord-341342-kyavg4vu 239 17 N n CD cord-341342-kyavg4vu 239 18 protein protein NN cord-341342-kyavg4vu 239 19 . . . cord-341342-kyavg4vu 240 1 Only only RB cord-341342-kyavg4vu 240 2 full full JJ cord-341342-kyavg4vu 240 3 - - HYPH cord-341342-kyavg4vu 240 4 length length NN cord-341342-kyavg4vu 240 5 genomic genomic JJ cord-341342-kyavg4vu 240 6 RNA rna NN cord-341342-kyavg4vu 240 7 of of IN cord-341342-kyavg4vu 240 8 MHV MHV NNP cord-341342-kyavg4vu 240 9 is be VBZ cord-341342-kyavg4vu 240 10 packaged package VBN cord-341342-kyavg4vu 240 11 into into IN cord-341342-kyavg4vu 240 12 virions virion NNS cord-341342-kyavg4vu 240 13 . . . cord-341342-kyavg4vu 241 1 Recently recently RB cord-341342-kyavg4vu 241 2 , , , cord-341342-kyavg4vu 241 3 analysis analysis NN cord-341342-kyavg4vu 241 4 of of IN cord-341342-kyavg4vu 241 5 defective defective JJ cord-341342-kyavg4vu 241 6 interfering interfere VBG cord-341342-kyavg4vu 241 7 particles particle NNS cord-341342-kyavg4vu 241 8 of of IN cord-341342-kyavg4vu 241 9 MHV MHV NNP cord-341342-kyavg4vu 241 10 has have VBZ cord-341342-kyavg4vu 241 11 identified identify VBN cord-341342-kyavg4vu 241 12 the the DT cord-341342-kyavg4vu 241 13 RNA RNA NNP cord-341342-kyavg4vu 241 14 packaging packaging NN cord-341342-kyavg4vu 241 15 signal signal NN cord-341342-kyavg4vu 241 16 as as IN cord-341342-kyavg4vu 241 17 falling fall VBG cord-341342-kyavg4vu 241 18 within within IN cord-341342-kyavg4vu 241 19 a a DT cord-341342-kyavg4vu 241 20 4(10 4(10 CD cord-341342-kyavg4vu 241 21 nucleotide nucleotide JJ cord-341342-kyavg4vu 241 22 locus locus NN cord-341342-kyavg4vu 241 23 near near IN cord-341342-kyavg4vu 241 24 the the DT cord-341342-kyavg4vu 241 25 3 3 CD cord-341342-kyavg4vu 241 26 ' ' NN cord-341342-kyavg4vu 241 27 end end NN cord-341342-kyavg4vu 241 28 of of IN cord-341342-kyavg4vu 241 29 the the DT cord-341342-kyavg4vu 241 30 huge huge JJ cord-341342-kyavg4vu 241 31 viral viral JJ cord-341342-kyavg4vu 241 32 polymerase polymerase NN cord-341342-kyavg4vu 241 33 gene gene NN cord-341342-kyavg4vu 241 34 [ [ -LRB- cord-341342-kyavg4vu 241 35 13 13 CD cord-341342-kyavg4vu 241 36 , , , cord-341342-kyavg4vu 241 37 33 33 CD cord-341342-kyavg4vu 241 38 ] ] -RRB- cord-341342-kyavg4vu 241 39 , , , cord-341342-kyavg4vu 241 40 which which WDT cord-341342-kyavg4vu 241 41 is be VBZ cord-341342-kyavg4vu 241 42 unique unique JJ cord-341342-kyavg4vu 241 43 to to TO cord-341342-kyavg4vu 241 44 genomic genomic JJ cord-341342-kyavg4vu 241 45 R r NN cord-341342-kyavg4vu 241 46 N n NN cord-341342-kyavg4vu 241 47 A a NN cord-341342-kyavg4vu 241 48 and and CC cord-341342-kyavg4vu 241 49 is be VBZ cord-341342-kyavg4vu 241 50 not not RB cord-341342-kyavg4vu 241 51 found find VBN cord-341342-kyavg4vu 241 52 in in IN cord-341342-kyavg4vu 241 53 subgenomic subgenomic NNP cord-341342-kyavg4vu 241 54 R R NNP cord-341342-kyavg4vu 241 55 N N NNP cord-341342-kyavg4vu 241 56 A A NNP cord-341342-kyavg4vu 241 57 species specie NNS cord-341342-kyavg4vu 241 58 . . . cord-341342-kyavg4vu 242 1 Others other NNS cord-341342-kyavg4vu 242 2 have have VBP cord-341342-kyavg4vu 242 3 reported report VBN cord-341342-kyavg4vu 242 4 sequence sequence NN cord-341342-kyavg4vu 242 5 - - HYPH cord-341342-kyavg4vu 242 6 specific specific JJ cord-341342-kyavg4vu 242 7 binding binding NN cord-341342-kyavg4vu 242 8 of of IN cord-341342-kyavg4vu 242 9 N n CD cord-341342-kyavg4vu 242 10 protein protein NN cord-341342-kyavg4vu 242 11 to to IN cord-341342-kyavg4vu 242 12 nucleotides nucleotide NNS cord-341342-kyavg4vu 242 13 56 56 CD cord-341342-kyavg4vu 242 14 to to TO cord-341342-kyavg4vu 242 15 65 65 CD cord-341342-kyavg4vu 242 16 of of IN cord-341342-kyavg4vu 242 17 the the DT cord-341342-kyavg4vu 242 18 M M NNP cord-341342-kyavg4vu 242 19 H h NN cord-341342-kyavg4vu 242 20 V v NN cord-341342-kyavg4vu 242 21 leader leader NN cord-341342-kyavg4vu 242 22 [ [ -LRB- cord-341342-kyavg4vu 242 23 29 29 CD cord-341342-kyavg4vu 242 24 ] ] -RRB- cord-341342-kyavg4vu 242 25 , , , cord-341342-kyavg4vu 242 26 which which WDT cord-341342-kyavg4vu 242 27 is be VBZ cord-341342-kyavg4vu 242 28 c c NNP cord-341342-kyavg4vu 242 29 o o NN cord-341342-kyavg4vu 242 30 m m NN cord-341342-kyavg4vu 242 31 m m NN cord-341342-kyavg4vu 242 32 o o NN cord-341342-kyavg4vu 242 33 n n NNP cord-341342-kyavg4vu 242 34 to to IN cord-341342-kyavg4vu 242 35 all all DT cord-341342-kyavg4vu 242 36 M m NN cord-341342-kyavg4vu 242 37 H h NN cord-341342-kyavg4vu 242 38 V v NN cord-341342-kyavg4vu 242 39 genomic genomic JJ cord-341342-kyavg4vu 242 40 and and CC cord-341342-kyavg4vu 242 41 subgenomic subgenomic JJ cord-341342-kyavg4vu 242 42 RNAs rna NNS cord-341342-kyavg4vu 242 43 . . . cord-341342-kyavg4vu 243 1 This this DT cord-341342-kyavg4vu 243 2 latter latter JJ cord-341342-kyavg4vu 243 3 sequence sequence NN cord-341342-kyavg4vu 243 4 was be VBD cord-341342-kyavg4vu 243 5 present present JJ cord-341342-kyavg4vu 243 6 on on IN cord-341342-kyavg4vu 243 7 many many JJ cord-341342-kyavg4vu 243 8 of of IN cord-341342-kyavg4vu 243 9 the the DT cord-341342-kyavg4vu 243 10 synthesized synthesize VBN cord-341342-kyavg4vu 243 11 m m NN cord-341342-kyavg4vu 243 12 R r NN cord-341342-kyavg4vu 243 13 N N NNP cord-341342-kyavg4vu 243 14 A A NNP cord-341342-kyavg4vu 243 15 s s NN cord-341342-kyavg4vu 243 16 used use VBN cord-341342-kyavg4vu 243 17 in in IN cord-341342-kyavg4vu 243 18 our -PRON- PRP$ cord-341342-kyavg4vu 243 19 study study NN cord-341342-kyavg4vu 243 20 , , , cord-341342-kyavg4vu 243 21 but but CC cord-341342-kyavg4vu 243 22 selective selective JJ cord-341342-kyavg4vu 243 23 binding binding NN cord-341342-kyavg4vu 243 24 to to IN cord-341342-kyavg4vu 243 25 this this DT cord-341342-kyavg4vu 243 26 R r NN cord-341342-kyavg4vu 243 27 N N NNP cord-341342-kyavg4vu 243 28 A A NNP cord-341342-kyavg4vu 243 29 region region NN cord-341342-kyavg4vu 243 30 was be VBD cord-341342-kyavg4vu 243 31 not not RB cord-341342-kyavg4vu 243 32 observed observe VBN cord-341342-kyavg4vu 243 33 ( ( -LRB- cord-341342-kyavg4vu 243 34 Fig Fig NNP cord-341342-kyavg4vu 243 35 . . . cord-341342-kyavg4vu 243 36 4 4 CD cord-341342-kyavg4vu 243 37 ) ) -RRB- cord-341342-kyavg4vu 243 38 . . . cord-341342-kyavg4vu 244 1 It -PRON- PRP cord-341342-kyavg4vu 244 2 is be VBZ cord-341342-kyavg4vu 244 3 possible possible JJ cord-341342-kyavg4vu 244 4 that that IN cord-341342-kyavg4vu 244 5 the the DT cord-341342-kyavg4vu 244 6 concentrations concentration NNS cord-341342-kyavg4vu 244 7 of of IN cord-341342-kyavg4vu 244 8 N n CD cord-341342-kyavg4vu 244 9 protein protein NN cord-341342-kyavg4vu 244 10 and and CC cord-341342-kyavg4vu 244 11 n n NN cord-341342-kyavg4vu 244 12 o o NN cord-341342-kyavg4vu 244 13 n n NN cord-341342-kyavg4vu 244 14 -M -m NN cord-341342-kyavg4vu 244 15 H h NN cord-341342-kyavg4vu 244 16 V v NN cord-341342-kyavg4vu 244 17 R r NN cord-341342-kyavg4vu 244 18 N n NN cord-341342-kyavg4vu 244 19 A a NN cord-341342-kyavg4vu 244 20 in in IN cord-341342-kyavg4vu 244 21 the the DT cord-341342-kyavg4vu 244 22 reticulocyte reticulocyte NN cord-341342-kyavg4vu 244 23 lysate lysate NN cord-341342-kyavg4vu 244 24 in in IN cord-341342-kyavg4vu 244 25 our -PRON- PRP$ cord-341342-kyavg4vu 244 26 assay assay NN cord-341342-kyavg4vu 244 27 were be VBD cord-341342-kyavg4vu 244 28 too too RB cord-341342-kyavg4vu 244 29 high high JJ cord-341342-kyavg4vu 244 30 to to TO cord-341342-kyavg4vu 244 31 allow allow VB cord-341342-kyavg4vu 244 32 demonstration demonstration NN cord-341342-kyavg4vu 244 33 of of IN cord-341342-kyavg4vu 244 34 a a DT cord-341342-kyavg4vu 244 35 selective selective JJ cord-341342-kyavg4vu 244 36 recognition recognition NN cord-341342-kyavg4vu 244 37 of of IN cord-341342-kyavg4vu 244 38 M M NNP cord-341342-kyavg4vu 244 39 H h NN cord-341342-kyavg4vu 244 40 V v NN cord-341342-kyavg4vu 244 41 R r NN cord-341342-kyavg4vu 244 42 N n NN cord-341342-kyavg4vu 244 43 A a NN cord-341342-kyavg4vu 244 44 . . . cord-341342-kyavg4vu 245 1 We -PRON- PRP cord-341342-kyavg4vu 245 2 are be VBP cord-341342-kyavg4vu 245 3 currently currently RB cord-341342-kyavg4vu 245 4 seeking seek VBG cord-341342-kyavg4vu 245 5 to to TO cord-341342-kyavg4vu 245 6 establish establish VB cord-341342-kyavg4vu 245 7 the the DT cord-341342-kyavg4vu 245 8 assay assay NN cord-341342-kyavg4vu 245 9 conditions condition NNS cord-341342-kyavg4vu 245 10 and and CC cord-341342-kyavg4vu 245 11 the the DT cord-341342-kyavg4vu 245 12 correct correct JJ cord-341342-kyavg4vu 245 13 R r NN cord-341342-kyavg4vu 245 14 N n NN cord-341342-kyavg4vu 245 15 A a NN cord-341342-kyavg4vu 245 16 substrate substrate NN cord-341342-kyavg4vu 245 17 to to TO cord-341342-kyavg4vu 245 18 examine examine VB cord-341342-kyavg4vu 245 19 sequence sequence NN cord-341342-kyavg4vu 245 20 - - HYPH cord-341342-kyavg4vu 245 21 specific specific JJ cord-341342-kyavg4vu 245 22 R r NN cord-341342-kyavg4vu 245 23 N n NN cord-341342-kyavg4vu 245 24 A a NN cord-341342-kyavg4vu 245 25 binding binding NN cord-341342-kyavg4vu 245 26 by by IN cord-341342-kyavg4vu 245 27 the the DT cord-341342-kyavg4vu 245 28 M M NNP cord-341342-kyavg4vu 245 29 H H NNP cord-341342-kyavg4vu 245 30 V v NN cord-341342-kyavg4vu 245 31 N n NN cord-341342-kyavg4vu 245 32 protein protein NN cord-341342-kyavg4vu 245 33 . . . cord-341342-kyavg4vu 246 1 Nucleocapsids nucleocapsid NNS cord-341342-kyavg4vu 246 2 of of IN cord-341342-kyavg4vu 246 3 large large JJ cord-341342-kyavg4vu 246 4 RNA RNA NNP cord-341342-kyavg4vu 246 5 viruses virus NNS cord-341342-kyavg4vu 246 6 as as RB cord-341342-kyavg4vu 246 7 functionally functionally RB cord-341342-kyavg4vu 246 8 active active JJ cord-341342-kyavg4vu 246 9 units unit NNS cord-341342-kyavg4vu 246 10 in in IN cord-341342-kyavg4vu 246 11 transcription transcription NN cord-341342-kyavg4vu 246 12 Coronavirus Coronavirus NNP cord-341342-kyavg4vu 246 13 IBV IBV NNP cord-341342-kyavg4vu 246 14 : : : cord-341342-kyavg4vu 246 15 partial partial JJ cord-341342-kyavg4vu 246 16 amino amino NN cord-341342-kyavg4vu 246 17 terminal terminal JJ cord-341342-kyavg4vu 246 18 sequencing sequencing NN cord-341342-kyavg4vu 246 19 of of IN cord-341342-kyavg4vu 246 20 spike spike NN cord-341342-kyavg4vu 246 21 polypeptide polypeptide NN cord-341342-kyavg4vu 246 22 $ $ $ cord-341342-kyavg4vu 246 23 2 2 CD cord-341342-kyavg4vu 246 24 identifies identify VBZ cord-341342-kyavg4vu 246 25 the the DT cord-341342-kyavg4vu 246 26 sequence sequence NN cord-341342-kyavg4vu 246 27 Arg arg NN cord-341342-kyavg4vu 246 28 - - HYPH cord-341342-kyavg4vu 246 29 Arg arg NN cord-341342-kyavg4vu 246 30 - - HYPH cord-341342-kyavg4vu 246 31 Phe phe NN cord-341342-kyavg4vu 246 32 - - HYPH cord-341342-kyavg4vu 246 33 Arg arg NN cord-341342-kyavg4vu 246 34 - - HYPH cord-341342-kyavg4vu 246 35 Arg arg NN cord-341342-kyavg4vu 246 36 at at IN cord-341342-kyavg4vu 246 37 the the DT cord-341342-kyavg4vu 246 38 cleavage cleavage NN cord-341342-kyavg4vu 246 39 site site NN cord-341342-kyavg4vu 246 40 of of IN cord-341342-kyavg4vu 246 41 the the DT cord-341342-kyavg4vu 246 42 spike spike NN cord-341342-kyavg4vu 246 43 precursor precursor NN cord-341342-kyavg4vu 246 44 propolypeptide propolypeptide NN cord-341342-kyavg4vu 246 45 of of IN cord-341342-kyavg4vu 246 46 IBV IBV NNP cord-341342-kyavg4vu 246 47 strains strain VBZ cord-341342-kyavg4vu 246 48 Beaudette Beaudette NNP cord-341342-kyavg4vu 246 49 and and CC cord-341342-kyavg4vu 246 50 M41 M41 NNP cord-341342-kyavg4vu 246 51 Heterogeneous heterogeneous JJ cord-341342-kyavg4vu 246 52 nuclear nuclear JJ cord-341342-kyavg4vu 246 53 ribonucleoprotein ribonucleoprotein NN cord-341342-kyavg4vu 246 54 particles particle NNS cord-341342-kyavg4vu 246 55 and and CC cord-341342-kyavg4vu 246 56 the the DT cord-341342-kyavg4vu 246 57 pathway pathway NN cord-341342-kyavg4vu 246 58 of of IN cord-341342-kyavg4vu 246 59 mRNA mRNA NNP cord-341342-kyavg4vu 246 60 formation formation NN cord-341342-kyavg4vu 246 61 Assembly Assembly NNP cord-341342-kyavg4vu 246 62 of of IN cord-341342-kyavg4vu 246 63 influenza influenza NN cord-341342-kyavg4vu 246 64 ribonucleoprotein ribonucleoprotein NN cord-341342-kyavg4vu 246 65 in in IN cord-341342-kyavg4vu 246 66 vitro vitro FW cord-341342-kyavg4vu 246 67 using use VBG cord-341342-kyavg4vu 246 68 recombinant recombinant JJ cord-341342-kyavg4vu 246 69 nucleoprotein nucleoprotein NNP cord-341342-kyavg4vu 246 70 Mechanism Mechanism NNP cord-341342-kyavg4vu 246 71 of of IN cord-341342-kyavg4vu 246 72 interferon interferon NNP cord-341342-kyavg4vu 246 73 action action NN cord-341342-kyavg4vu 246 74 . . . cord-341342-kyavg4vu 247 1 Interferon interferon NN cord-341342-kyavg4vu 247 2 - - HYPH cord-341342-kyavg4vu 247 3 mediated mediate VBN cord-341342-kyavg4vu 247 4 inhibition inhibition NN cord-341342-kyavg4vu 247 5 of of IN cord-341342-kyavg4vu 247 6 simian simian JJ cord-341342-kyavg4vu 247 7 virus-40 virus-40 NNS cord-341342-kyavg4vu 247 8 early early JJ cord-341342-kyavg4vu 247 9 RNA RNA NNP cord-341342-kyavg4vu 247 10 accumulation accumulation NN cord-341342-kyavg4vu 247 11 Zinc Zinc NNP cord-341342-kyavg4vu 247 12 fingers finger NNS cord-341342-kyavg4vu 247 13 " " '' cord-341342-kyavg4vu 247 14 : : : cord-341342-kyavg4vu 247 15 a a DT cord-341342-kyavg4vu 247 16 novel novel JJ cord-341342-kyavg4vu 247 17 protein protein NN cord-341342-kyavg4vu 247 18 motif motif NN cord-341342-kyavg4vu 247 19 for for IN cord-341342-kyavg4vu 247 20 nucleic nucleic JJ cord-341342-kyavg4vu 247 21 acid acid NN cord-341342-kyavg4vu 247 22 recognition recognition NN cord-341342-kyavg4vu 247 23 Cleavage cleavage NN cord-341342-kyavg4vu 247 24 of of IN cord-341342-kyavg4vu 247 25 structural structural JJ cord-341342-kyavg4vu 247 26 proteins protein NNS cord-341342-kyavg4vu 247 27 during during IN cord-341342-kyavg4vu 247 28 the the DT cord-341342-kyavg4vu 247 29 assembly assembly NN cord-341342-kyavg4vu 247 30 of of IN cord-341342-kyavg4vu 247 31 the the DT cord-341342-kyavg4vu 247 32 head head NN cord-341342-kyavg4vu 247 33 of of IN cord-341342-kyavg4vu 247 34 bacteriophage bacteriophage NN cord-341342-kyavg4vu 247 35 T4 T4 NNP cord-341342-kyavg4vu 247 36 Characterization Characterization NNP cord-341342-kyavg4vu 247 37 of of IN cord-341342-kyavg4vu 247 38 leader leader NN cord-341342-kyavg4vu 247 39 RNA RNA NNP cord-341342-kyavg4vu 247 40 sequences sequence NNS cord-341342-kyavg4vu 247 41 on on IN cord-341342-kyavg4vu 247 42 the the DT cord-341342-kyavg4vu 247 43 virion virion NN cord-341342-kyavg4vu 247 44 and and CC cord-341342-kyavg4vu 247 45 mRNAs mrnas CD cord-341342-kyavg4vu 247 46 of of IN cord-341342-kyavg4vu 247 47 mouse mouse NNP cord-341342-kyavg4vu 247 48 hepatitis hepatitis NNP cord-341342-kyavg4vu 247 49 virus virus NN cord-341342-kyavg4vu 247 50 , , , cord-341342-kyavg4vu 247 51 a a DT cord-341342-kyavg4vu 247 52 cytoplasmic cytoplasmic JJ cord-341342-kyavg4vu 247 53 RNA RNA NNP cord-341342-kyavg4vu 247 54 virus virus NN cord-341342-kyavg4vu 247 55 Sequence sequence NN cord-341342-kyavg4vu 247 56 - - HYPH cord-341342-kyavg4vu 247 57 specific specific JJ cord-341342-kyavg4vu 247 58 recognition recognition NN cord-341342-kyavg4vu 247 59 of of IN cord-341342-kyavg4vu 247 60 RNA RNA NNP cord-341342-kyavg4vu 247 61 hairpins hairpin NNS cord-341342-kyavg4vu 247 62 by by IN cord-341342-kyavg4vu 247 63 bacteriophage bacteriophage NN cord-341342-kyavg4vu 247 64 antiterminators antiterminator NNS cord-341342-kyavg4vu 247 65 requires require VBZ cord-341342-kyavg4vu 247 66 a a DT cord-341342-kyavg4vu 247 67 conserved conserve VBN cord-341342-kyavg4vu 247 68 arginine arginine NN cord-341342-kyavg4vu 247 69 - - HYPH cord-341342-kyavg4vu 247 70 rich rich JJ cord-341342-kyavg4vu 247 71 motif motif NN cord-341342-kyavg4vu 248 1 The the DT cord-341342-kyavg4vu 248 2 complete complete JJ cord-341342-kyavg4vu 248 3 sequence sequence NN cord-341342-kyavg4vu 248 4 ( ( -LRB- cord-341342-kyavg4vu 248 5 22 22 CD cord-341342-kyavg4vu 248 6 kilobases kilobase NNS cord-341342-kyavg4vu 248 7 ) ) -RRB- cord-341342-kyavg4vu 248 8 of of IN cord-341342-kyavg4vu 248 9 murine murine JJ cord-341342-kyavg4vu 248 10 coronavirus coronavirus NN cord-341342-kyavg4vu 248 11 gene gene NN cord-341342-kyavg4vu 248 12 I -PRON- PRP cord-341342-kyavg4vu 248 13 encoding encode VBG cord-341342-kyavg4vu 248 14 the the DT cord-341342-kyavg4vu 248 15 putative putative JJ cord-341342-kyavg4vu 248 16 proteases protease NNS cord-341342-kyavg4vu 248 17 and and CC cord-341342-kyavg4vu 248 18 RNA RNA NNP cord-341342-kyavg4vu 248 19 polymerase polymerase NN cord-341342-kyavg4vu 249 1 Plus plus CC cord-341342-kyavg4vu 249 2 and and CC cord-341342-kyavg4vu 249 3 minus minus CC cord-341342-kyavg4vu 249 4 strand strand NN cord-341342-kyavg4vu 249 5 leader leader NN cord-341342-kyavg4vu 249 6 RNAs rna NNS cord-341342-kyavg4vu 249 7 in in IN cord-341342-kyavg4vu 249 8 negative negative JJ cord-341342-kyavg4vu 249 9 strand strand NN cord-341342-kyavg4vu 249 10 virus virus NN cord-341342-kyavg4vu 249 11 - - HYPH cord-341342-kyavg4vu 249 12 infected infect VBN cord-341342-kyavg4vu 249 13 cells cell NNS cord-341342-kyavg4vu 249 14 Ribonucleoprotein Ribonucleoprotein NNP cord-341342-kyavg4vu 249 15 - - HYPH cord-341342-kyavg4vu 249 16 like like JJ cord-341342-kyavg4vu 249 17 structures structure NNS cord-341342-kyavg4vu 249 18 from from IN cord-341342-kyavg4vu 249 19 coronavirus coronavirus NN cord-341342-kyavg4vu 249 20 particles particle NNS cord-341342-kyavg4vu 249 21 RNA rna NN cord-341342-kyavg4vu 249 22 - - HYPH cord-341342-kyavg4vu 249 23 binding bind VBG cord-341342-kyavg4vu 249 24 domain domain NN cord-341342-kyavg4vu 249 25 of of IN cord-341342-kyavg4vu 249 26 MHV MHV NNP cord-341342-kyavg4vu 249 27 N N NNP cord-341342-kyavg4vu 249 28 protein protein NN cord-341342-kyavg4vu 249 29 159 159 CD cord-341342-kyavg4vu 249 30 Analysis Analysis NNP cord-341342-kyavg4vu 249 31 of of IN cord-341342-kyavg4vu 249 32 efficiently efficiently RB cord-341342-kyavg4vu 249 33 packaged package VBN cord-341342-kyavg4vu 249 34 defective defective JJ cord-341342-kyavg4vu 249 35 interfering interfere VBG cord-341342-kyavg4vu 249 36 RNAs rna NNS cord-341342-kyavg4vu 249 37 of of IN cord-341342-kyavg4vu 249 38 murine murine JJ cord-341342-kyavg4vu 249 39 coronavirus coronavirus NN cord-341342-kyavg4vu 249 40 : : : cord-341342-kyavg4vu 249 41 localization localization NN cord-341342-kyavg4vu 249 42 of of IN cord-341342-kyavg4vu 249 43 a a DT cord-341342-kyavg4vu 249 44 possible possible JJ cord-341342-kyavg4vu 249 45 packaging packaging NN cord-341342-kyavg4vu 249 46 signal signal NN cord-341342-kyavg4vu 249 47 Molecular molecular JJ cord-341342-kyavg4vu 249 48 cloning cloning NN cord-341342-kyavg4vu 249 49 : : : cord-341342-kyavg4vu 250 1 a a DT cord-341342-kyavg4vu 250 2 laboratory laboratory NN cord-341342-kyavg4vu 250 3 manual manual NN cord-341342-kyavg4vu 250 4 Resolution resolution NN cord-341342-kyavg4vu 250 5 of of IN cord-341342-kyavg4vu 250 6 multiple multiple JJ cord-341342-kyavg4vu 250 7 complexes complex NNS cord-341342-kyavg4vu 250 8 of of IN cord-341342-kyavg4vu 250 9 phosphoprotein phosphoprotein NN cord-341342-kyavg4vu 250 10 NS NS NNP cord-341342-kyavg4vu 250 11 with with IN cord-341342-kyavg4vu 250 12 nucleocapsid nucleocapsid NNP cord-341342-kyavg4vu 250 13 protein protein NNP cord-341342-kyavg4vu 250 14 N n CD cord-341342-kyavg4vu 250 15 of of IN cord-341342-kyavg4vu 250 16 vesicular vesicular JJ cord-341342-kyavg4vu 250 17 stomatitis stomatitis NN cord-341342-kyavg4vu 250 18 virus virus NN cord-341342-kyavg4vu 250 19 Complex complex JJ cord-341342-kyavg4vu 250 20 formation formation NN cord-341342-kyavg4vu 250 21 with with IN cord-341342-kyavg4vu 250 22 vesicular vesicular JJ cord-341342-kyavg4vu 250 23 stomatitis stomatitis NN cord-341342-kyavg4vu 250 24 virus virus NN cord-341342-kyavg4vu 250 25 phosphoprotein phosphoprotein NN cord-341342-kyavg4vu 250 26 NS NS NNP cord-341342-kyavg4vu 250 27 prevents prevent VBZ cord-341342-kyavg4vu 250 28 binding binding NN cord-341342-kyavg4vu 250 29 of of IN cord-341342-kyavg4vu 250 30 nucleocapsid nucleocapsid NNP cord-341342-kyavg4vu 250 31 protein protein NN cord-341342-kyavg4vu 250 32 N n CD cord-341342-kyavg4vu 250 33 to to IN cord-341342-kyavg4vu 250 34 nonspecific nonspecific JJ cord-341342-kyavg4vu 250 35 RNA RNA NNP cord-341342-kyavg4vu 250 36 Structure Structure NNP cord-341342-kyavg4vu 250 37 and and CC cord-341342-kyavg4vu 250 38 function function NN cord-341342-kyavg4vu 250 39 studies study NNS cord-341342-kyavg4vu 250 40 of of IN cord-341342-kyavg4vu 250 41 the the DT cord-341342-kyavg4vu 250 42 nucleocapsid nucleocapsid NNP cord-341342-kyavg4vu 250 43 protein protein NN cord-341342-kyavg4vu 250 44 of of IN cord-341342-kyavg4vu 250 45 mouse mouse NNP cord-341342-kyavg4vu 250 46 hepatitis hepatitis NNP cord-341342-kyavg4vu 250 47 virus virus NN cord-341342-kyavg4vu 250 48 Efficient efficient JJ cord-341342-kyavg4vu 250 49 in in IN cord-341342-kyavg4vu 250 50 vitro vitro FW cord-341342-kyavg4vu 250 51 synthesis synthesis NN cord-341342-kyavg4vu 250 52 of of IN cord-341342-kyavg4vu 250 53 biological biological JJ cord-341342-kyavg4vu 250 54 active active JJ cord-341342-kyavg4vu 250 55 RNA RNA NNP cord-341342-kyavg4vu 250 56 and and CC cord-341342-kyavg4vu 250 57 RNA RNA NNP cord-341342-kyavg4vu 250 58 hybridization hybridization NN cord-341342-kyavg4vu 250 59 probes probe NNS cord-341342-kyavg4vu 250 60 from from IN cord-341342-kyavg4vu 250 61 plasmids plasmid NNS cord-341342-kyavg4vu 250 62 containing contain VBG cord-341342-kyavg4vu 250 63 a a DT cord-341342-kyavg4vu 250 64 bacteriophage bacteriophage NN cord-341342-kyavg4vu 250 65 SP6 sp6 NN cord-341342-kyavg4vu 250 66 promoter promoter NN cord-341342-kyavg4vu 250 67 Molecular molecular JJ cord-341342-kyavg4vu 250 68 cloning cloning NN cord-341342-kyavg4vu 250 69 of of IN cord-341342-kyavg4vu 250 70 the the DT cord-341342-kyavg4vu 250 71 gene gene NN cord-341342-kyavg4vu 250 72 encoding encode VBG cord-341342-kyavg4vu 250 73 the the DT cord-341342-kyavg4vu 250 74 putative putative JJ cord-341342-kyavg4vu 250 75 polymerase polymerase NN cord-341342-kyavg4vu 250 76 of of IN cord-341342-kyavg4vu 250 77 mouse mouse NN cord-341342-kyavg4vu 250 78 hepatitis hepatitis NN cord-341342-kyavg4vu 250 79 coronavirus coronavirus NN cord-341342-kyavg4vu 250 80 , , , cord-341342-kyavg4vu 250 81 strain strain VB cord-341342-kyavg4vu 250 82 A59 A59 NNP cord-341342-kyavg4vu 250 83 Sequence sequence NN cord-341342-kyavg4vu 250 84 comparison comparison NN cord-341342-kyavg4vu 250 85 of of IN cord-341342-kyavg4vu 250 86 the the DT cord-341342-kyavg4vu 250 87 N n CD cord-341342-kyavg4vu 250 88 genes gene NNS cord-341342-kyavg4vu 250 89 of of IN cord-341342-kyavg4vu 250 90 five five CD cord-341342-kyavg4vu 250 91 strains strain NNS cord-341342-kyavg4vu 250 92 of of IN cord-341342-kyavg4vu 250 93 the the DT cord-341342-kyavg4vu 250 94 coronavirus coronavirus NN cord-341342-kyavg4vu 250 95 mouse mouse NN cord-341342-kyavg4vu 250 96 hepatitis hepatitis NN cord-341342-kyavg4vu 250 97 virus virus NN cord-341342-kyavg4vu 251 1 suggests suggest VBZ cord-341342-kyavg4vu 251 2 a a DT cord-341342-kyavg4vu 251 3 three three CD cord-341342-kyavg4vu 251 4 domain domain NN cord-341342-kyavg4vu 251 5 structure structure NN cord-341342-kyavg4vu 251 6 for for IN cord-341342-kyavg4vu 251 7 the the DT cord-341342-kyavg4vu 251 8 nucleocapsid nucleocapsid NNP cord-341342-kyavg4vu 251 9 protein protein NN cord-341342-kyavg4vu 251 10 A a DT cord-341342-kyavg4vu 251 11 common common JJ cord-341342-kyavg4vu 251 12 RNA RNA NNP cord-341342-kyavg4vu 251 13 recognition recognition NN cord-341342-kyavg4vu 251 14 motif motif NN cord-341342-kyavg4vu 251 15 identified identify VBN cord-341342-kyavg4vu 251 16 within within IN cord-341342-kyavg4vu 251 17 a a DT cord-341342-kyavg4vu 251 18 defined define VBN cord-341342-kyavg4vu 251 19 U1 U1 NNP cord-341342-kyavg4vu 251 20 RNA rna NN cord-341342-kyavg4vu 251 21 binding binding NN cord-341342-kyavg4vu 251 22 domain domain NN cord-341342-kyavg4vu 251 23 of of IN cord-341342-kyavg4vu 251 24 the the DT cord-341342-kyavg4vu 251 25 70 70 CD cord-341342-kyavg4vu 251 26 K K NNP cord-341342-kyavg4vu 251 27 U1 U1 NNP cord-341342-kyavg4vu 251 28 snRNP snrnp NN cord-341342-kyavg4vu 251 29 protein protein NN cord-341342-kyavg4vu 251 30 RNA rna NN cord-341342-kyavg4vu 251 31 - - HYPH cord-341342-kyavg4vu 251 32 binding bind VBG cord-341342-kyavg4vu 251 33 proteins protein NNS cord-341342-kyavg4vu 251 34 of of IN cord-341342-kyavg4vu 251 35 coronavirus coronavirus NN cord-341342-kyavg4vu 251 36 MHV mhv NN cord-341342-kyavg4vu 251 37 : : : cord-341342-kyavg4vu 251 38 detection detection NN cord-341342-kyavg4vu 251 39 of of IN cord-341342-kyavg4vu 251 40 monomeric monomeric JJ cord-341342-kyavg4vu 251 41 and and CC cord-341342-kyavg4vu 251 42 multimeric multimeric JJ cord-341342-kyavg4vu 251 43 N n CD cord-341342-kyavg4vu 251 44 protein protein NN cord-341342-kyavg4vu 251 45 with with IN cord-341342-kyavg4vu 251 46 an an DT cord-341342-kyavg4vu 251 47 RNA RNA NNP cord-341342-kyavg4vu 251 48 overlay overlay NN cord-341342-kyavg4vu 251 49 - - HYPH cord-341342-kyavg4vu 251 50 protein protein NN cord-341342-kyavg4vu 251 51 blot blot NN cord-341342-kyavg4vu 251 52 assay assay NN cord-341342-kyavg4vu 252 1 The the DT cord-341342-kyavg4vu 252 2 54-kD 54-kd CD cord-341342-kyavg4vu 252 3 protein protein NN cord-341342-kyavg4vu 252 4 of of IN cord-341342-kyavg4vu 252 5 signal signal NN cord-341342-kyavg4vu 252 6 recognition recognition NN cord-341342-kyavg4vu 252 7 particle particle NN cord-341342-kyavg4vu 252 8 contains contain VBZ cord-341342-kyavg4vu 252 9 a a DT cord-341342-kyavg4vu 252 10 methionine methionine JJ cord-341342-kyavg4vu 252 11 - - HYPH cord-341342-kyavg4vu 252 12 rich rich JJ cord-341342-kyavg4vu 252 13 RNA RNA NNP cord-341342-kyavg4vu 252 14 binding binding NN cord-341342-kyavg4vu 252 15 domain domain NN cord-341342-kyavg4vu 252 16 DNA dna NN cord-341342-kyavg4vu 252 17 sequencing sequencing NN cord-341342-kyavg4vu 252 18 with with IN cord-341342-kyavg4vu 252 19 chain chain NN cord-341342-kyavg4vu 252 20 terminating terminate VBG cord-341342-kyavg4vu 252 21 inhibitors inhibitor NNS cord-341342-kyavg4vu 252 22 Identification identification NN cord-341342-kyavg4vu 252 23 and and CC cord-341342-kyavg4vu 252 24 primary primary JJ cord-341342-kyavg4vu 252 25 structure structure NN cord-341342-kyavg4vu 252 26 of of IN cord-341342-kyavg4vu 252 27 the the DT cord-341342-kyavg4vu 252 28 gene gene NN cord-341342-kyavg4vu 252 29 encoding encode VBG cord-341342-kyavg4vu 252 30 the the DT cord-341342-kyavg4vu 252 31 Berne Berne NNP cord-341342-kyavg4vu 252 32 virus virus NN cord-341342-kyavg4vu 252 33 nucleocapsid nucleocapsid NN cord-341342-kyavg4vu 252 34 protein protein NN cord-341342-kyavg4vu 252 35 Coronaviruses Coronaviruses NNP cord-341342-kyavg4vu 252 36 : : : cord-341342-kyavg4vu 253 1 structure structure NN cord-341342-kyavg4vu 253 2 and and CC cord-341342-kyavg4vu 253 3 genome genome NN cord-341342-kyavg4vu 253 4 expression expression NN cord-341342-kyavg4vu 253 5 Assembly Assembly NNP cord-341342-kyavg4vu 253 6 of of IN cord-341342-kyavg4vu 253 7 nucleocapsidlike nucleocapsidlike NNP cord-341342-kyavg4vu 253 8 struct struct NNP cord-341342-kyavg4vu 253 9 ~ ~ NNP cord-341342-kyavg4vu 253 10 es es XX cord-341342-kyavg4vu 253 11 in in IN cord-341342-kyavg4vu 253 12 animal animal NN cord-341342-kyavg4vu 253 13 cells cell NNS cord-341342-kyavg4vu 253 14 infected infect VBN cord-341342-kyavg4vu 253 15 with with IN cord-341342-kyavg4vu 253 16 a a DT cord-341342-kyavg4vu 253 17 vaccinia vaccinia NN cord-341342-kyavg4vu 253 18 virus virus NN cord-341342-kyavg4vu 253 19 recombinant recombinant JJ cord-341342-kyavg4vu 253 20 encoding encode VBG cord-341342-kyavg4vu 253 21 the the DT cord-341342-kyavg4vu 253 22 measles measle NNS cord-341342-kyavg4vu 253 23 virus virus NN cord-341342-kyavg4vu 253 24 nucleoprorein nucleoprorein NNP cord-341342-kyavg4vu 253 25 Expression Expression NNP cord-341342-kyavg4vu 253 26 of of IN cord-341342-kyavg4vu 253 27 a a DT cord-341342-kyavg4vu 253 28 recombinant recombinant JJ cord-341342-kyavg4vu 253 29 DNA dna NN cord-341342-kyavg4vu 253 30 gene gene NN cord-341342-kyavg4vu 253 31 coding coding NN cord-341342-kyavg4vu 253 32 for for IN cord-341342-kyavg4vu 253 33 the the DT cord-341342-kyavg4vu 253 34 vesicular vesicular JJ cord-341342-kyavg4vu 253 35 stomatitis stomatitis NN cord-341342-kyavg4vu 253 36 virus virus NN cord-341342-kyavg4vu 253 37 nucleocapsid nucleocapsid NN cord-341342-kyavg4vu 253 38 protein protein NN cord-341342-kyavg4vu 253 39 Deans Deans NNP cord-341342-kyavg4vu 253 40 ILl ILl NNP cord-341342-kyavg4vu 253 41 ( ( -LRB- cord-341342-kyavg4vu 253 42 1988 1988 CD cord-341342-kyavg4vu 253 43 ) ) -RRB- cord-341342-kyavg4vu 254 1 Specific specific JJ cord-341342-kyavg4vu 254 2 interaction interaction NN cord-341342-kyavg4vu 254 3 between between IN cord-341342-kyavg4vu 254 4 coronavirus coronavirus NN cord-341342-kyavg4vu 254 5 leader leader NN cord-341342-kyavg4vu 254 6 RNA RNA NNP cord-341342-kyavg4vu 254 7 and and CC cord-341342-kyavg4vu 254 8 nucleocapsid nucleocapsid NN cord-341342-kyavg4vu 254 9 protein protein NN cord-341342-kyavg4vu 254 10 Synthesis Synthesis NNP cord-341342-kyavg4vu 254 11 and and CC cord-341342-kyavg4vu 254 12 subcellular subcellular JJ cord-341342-kyavg4vu 254 13 localization localization NN cord-341342-kyavg4vu 254 14 of of IN cord-341342-kyavg4vu 254 15 the the DT cord-341342-kyavg4vu 254 16 murine murine JJ cord-341342-kyavg4vu 254 17 coronavirus coronavirus NN cord-341342-kyavg4vu 254 18 nucleocapsid nucleocapsid NN cord-341342-kyavg4vu 254 19 protein protein NN cord-341342-kyavg4vu 254 20 The the DT cord-341342-kyavg4vu 254 21 molecular molecular JJ cord-341342-kyavg4vu 254 22 biology biology NN cord-341342-kyavg4vu 254 23 of of IN cord-341342-kyavg4vu 254 24 coronaviruses coronaviruse NNS cord-341342-kyavg4vu 255 1 I -PRON- PRP cord-341342-kyavg4vu 255 2 thank thank VBP cord-341342-kyavg4vu 255 3 Lawrence Lawrence NNP cord-341342-kyavg4vu 255 4 Sturman Sturman NNP cord-341342-kyavg4vu 255 5 for for IN cord-341342-kyavg4vu 255 6 numerous numerous JJ cord-341342-kyavg4vu 255 7 valuable valuable JJ cord-341342-kyavg4vu 255 8 discussions discussion NNS cord-341342-kyavg4vu 255 9 and and CC cord-341342-kyavg4vu 255 10 Cynthia Cynthia NNP cord-341342-kyavg4vu 255 11 Ricard Ricard NNP cord-341342-kyavg4vu 255 12 , , , cord-341342-kyavg4vu 255 13 who who WP cord-341342-kyavg4vu 255 14 first first RB cord-341342-kyavg4vu 255 15 suggested suggest VBD cord-341342-kyavg4vu 255 16 the the DT cord-341342-kyavg4vu 255 17 RNase rnase NN cord-341342-kyavg4vu 255 18 experiment experiment NN cord-341342-kyavg4vu 255 19 . . . cord-341342-kyavg4vu 256 1 I -PRON- PRP cord-341342-kyavg4vu 256 2 am be VBP cord-341342-kyavg4vu 256 3 grateful grateful JJ cord-341342-kyavg4vu 256 4 to to IN cord-341342-kyavg4vu 256 5 Monica Monica NNP cord-341342-kyavg4vu 256 6 Parker Parker NNP cord-341342-kyavg4vu 256 7 for for IN cord-341342-kyavg4vu 256 8 expert expert JJ cord-341342-kyavg4vu 256 9 technical technical JJ cord-341342-kyavg4vu 256 10 assistance assistance NN cord-341342-kyavg4vu 256 11 and and CC cord-341342-kyavg4vu 256 12 to to IN cord-341342-kyavg4vu 256 13 Arlene Arlene NNP cord-341342-kyavg4vu 256 14 Ramsingh Ramsingh NNP cord-341342-kyavg4vu 256 15 for for IN cord-341342-kyavg4vu 256 16 critically critically RB cord-341342-kyavg4vu 256 17 reading read VBG cord-341342-kyavg4vu 256 18 the the DT cord-341342-kyavg4vu 256 19 manuscript manuscript NN cord-341342-kyavg4vu 256 20 . . . cord-341342-kyavg4vu 257 1 This this DT cord-341342-kyavg4vu 257 2 work work NN cord-341342-kyavg4vu 257 3 was be VBD cord-341342-kyavg4vu 257 4 supported support VBN cord-341342-kyavg4vu 257 5 in in IN cord-341342-kyavg4vu 257 6 part part NN cord-341342-kyavg4vu 257 7 by by IN cord-341342-kyavg4vu 257 8 Public Public NNP cord-341342-kyavg4vu 257 9 Health Health NNP cord-341342-kyavg4vu 257 10 Service Service NNP cord-341342-kyavg4vu 257 11 grant grant NN cord-341342-kyavg4vu 257 12 GM31698 gm31698 NN cord-341342-kyavg4vu 257 13 from from IN cord-341342-kyavg4vu 257 14 the the DT cord-341342-kyavg4vu 257 15 National National NNP cord-341342-kyavg4vu 257 16 Institutes Institutes NNPS cord-341342-kyavg4vu 257 17 of of IN cord-341342-kyavg4vu 257 18 Health Health NNP cord-341342-kyavg4vu 257 19 . . .