id sid tid token lemma pos cord-343256-n593kh7u 1 1 key key JJ cord-343256-n593kh7u 1 2 : : : cord-343256-n593kh7u 1 3 cord-343256-n593kh7u cord-343256-n593kh7u NN cord-343256-n593kh7u 1 4 authors author NNS cord-343256-n593kh7u 1 5 : : : cord-343256-n593kh7u 1 6 Percy Percy NNP cord-343256-n593kh7u 1 7 , , , cord-343256-n593kh7u 1 8 D. D. NNP cord-343256-n593kh7u 1 9 H. H. NNP cord-343256-n593kh7u 1 10 ; ; : cord-343256-n593kh7u 1 11 Williams Williams NNP cord-343256-n593kh7u 1 12 , , , cord-343256-n593kh7u 1 13 K. K. NNP cord-343256-n593kh7u 1 14 L. L. NNP cord-343256-n593kh7u 1 15 ; ; : cord-343256-n593kh7u 1 16 Paturzo Paturzo NNP cord-343256-n593kh7u 1 17 , , , cord-343256-n593kh7u 2 1 F. F. NNP cord-343256-n593kh7u 2 2 X. X. NNP cord-343256-n593kh7u 2 3 title title NN cord-343256-n593kh7u 2 4 : : : cord-343256-n593kh7u 3 1 A a DT cord-343256-n593kh7u 3 2 comparison comparison NN cord-343256-n593kh7u 3 3 of of IN cord-343256-n593kh7u 3 4 the the DT cord-343256-n593kh7u 3 5 sensitivity sensitivity NN cord-343256-n593kh7u 3 6 and and CC cord-343256-n593kh7u 3 7 specificity specificity NN cord-343256-n593kh7u 3 8 of of IN cord-343256-n593kh7u 3 9 sialodacryoadenitis sialodacryoadenitis NNP cord-343256-n593kh7u 3 10 virus virus NN cord-343256-n593kh7u 3 11 , , , cord-343256-n593kh7u 3 12 Parker Parker NNP cord-343256-n593kh7u 3 13 's 's POS cord-343256-n593kh7u 3 14 rat rat NN cord-343256-n593kh7u 3 15 coronavirus coronavirus NN cord-343256-n593kh7u 3 16 , , , cord-343256-n593kh7u 3 17 and and CC cord-343256-n593kh7u 3 18 mouse mouse NN cord-343256-n593kh7u 3 19 hepatitis hepatitis NNP cord-343256-n593kh7u 3 20 virus virus NN cord-343256-n593kh7u 3 21 - - HYPH cord-343256-n593kh7u 3 22 infected infect VBN cord-343256-n593kh7u 3 23 cells cell NNS cord-343256-n593kh7u 3 24 as as IN cord-343256-n593kh7u 3 25 a a DT cord-343256-n593kh7u 3 26 source source NN cord-343256-n593kh7u 3 27 of of IN cord-343256-n593kh7u 3 28 antigen antigen NN cord-343256-n593kh7u 3 29 for for IN cord-343256-n593kh7u 3 30 the the DT cord-343256-n593kh7u 3 31 detection detection NN cord-343256-n593kh7u 3 32 of of IN cord-343256-n593kh7u 3 33 antibody antibody NN cord-343256-n593kh7u 3 34 to to IN cord-343256-n593kh7u 3 35 rat rat NN cord-343256-n593kh7u 3 36 coronaviruses coronaviruses NNP cord-343256-n593kh7u 3 37 date date NN cord-343256-n593kh7u 3 38 : : : cord-343256-n593kh7u 3 39 1991 1991 CD cord-343256-n593kh7u 3 40 journal journal NN cord-343256-n593kh7u 3 41 : : : cord-343256-n593kh7u 4 1 Arch arch JJ cord-343256-n593kh7u 4 2 Virol Virol NNP cord-343256-n593kh7u 4 3 DOI doi NN cord-343256-n593kh7u 4 4 : : : cord-343256-n593kh7u 5 1 10.1007 10.1007 CD cord-343256-n593kh7u 5 2 / / SYM cord-343256-n593kh7u 5 3 bf01310668 bf01310668 NNP cord-343256-n593kh7u 5 4 sha sha NNP cord-343256-n593kh7u 5 5 : : : cord-343256-n593kh7u 5 6 e58c81c04230122f8985724c18587739a0d69b40 e58c81c04230122f8985724c18587739a0d69b40 NNP cord-343256-n593kh7u 5 7 doc_id doc_id CD cord-343256-n593kh7u 5 8 : : : cord-343256-n593kh7u 5 9 343256 343256 CD cord-343256-n593kh7u 5 10 cord_uid cord_uid NN cord-343256-n593kh7u 5 11 : : : cord-343256-n593kh7u 6 1 n593kh7u n593kh7u ADD cord-343256-n593kh7u 7 1 Sialodacryoadenitis sialodacryoadenitis JJ cord-343256-n593kh7u 7 2 virus virus NN cord-343256-n593kh7u 7 3 ( ( -LRB- cord-343256-n593kh7u 7 4 SDAV SDAV NNP cord-343256-n593kh7u 7 5 ) ) -RRB- cord-343256-n593kh7u 7 6 and and CC cord-343256-n593kh7u 7 7 Parker Parker NNP cord-343256-n593kh7u 7 8 's 's POS cord-343256-n593kh7u 7 9 rat rat NN cord-343256-n593kh7u 7 10 coronavirus coronavirus NN cord-343256-n593kh7u 7 11 ( ( -LRB- cord-343256-n593kh7u 7 12 PRC PRC NNP cord-343256-n593kh7u 7 13 ) ) -RRB- cord-343256-n593kh7u 7 14 are be VBP cord-343256-n593kh7u 7 15 two two CD cord-343256-n593kh7u 7 16 recognized recognize VBN cord-343256-n593kh7u 7 17 viral viral JJ cord-343256-n593kh7u 7 18 strains strain NNS cord-343256-n593kh7u 7 19 which which WDT cord-343256-n593kh7u 7 20 cause cause VBP cord-343256-n593kh7u 7 21 spontaneous spontaneous JJ cord-343256-n593kh7u 7 22 disease disease NN cord-343256-n593kh7u 7 23 in in IN cord-343256-n593kh7u 7 24 the the DT cord-343256-n593kh7u 7 25 laboratory laboratory NN cord-343256-n593kh7u 7 26 rat rat NN cord-343256-n593kh7u 7 27 . . . cord-343256-n593kh7u 8 1 Currently currently RB cord-343256-n593kh7u 8 2 there there EX cord-343256-n593kh7u 8 3 is be VBZ cord-343256-n593kh7u 8 4 no no DT cord-343256-n593kh7u 8 5 recognized recognize VBN cord-343256-n593kh7u 8 6 practical practical JJ cord-343256-n593kh7u 8 7 procedure procedure NN cord-343256-n593kh7u 8 8 which which WDT cord-343256-n593kh7u 8 9 will will MD cord-343256-n593kh7u 8 10 accurately accurately RB cord-343256-n593kh7u 8 11 differentiate differentiate VB cord-343256-n593kh7u 8 12 infections infection NNS cord-343256-n593kh7u 8 13 with with IN cord-343256-n593kh7u 8 14 these these DT cord-343256-n593kh7u 8 15 strains strain NNS cord-343256-n593kh7u 8 16 . . . cord-343256-n593kh7u 9 1 Using use VBG cord-343256-n593kh7u 9 2 SDAV- SDAV- NNP cord-343256-n593kh7u 9 3 and and CC cord-343256-n593kh7u 9 4 PRC PRC NNP cord-343256-n593kh7u 9 5 - - HYPH cord-343256-n593kh7u 9 6 infected infect VBN cord-343256-n593kh7u 9 7 L-2 L-2 NNP cord-343256-n593kh7u 9 8 cells cell NNS cord-343256-n593kh7u 9 9 as as IN cord-343256-n593kh7u 9 10 the the DT cord-343256-n593kh7u 9 11 source source NN cord-343256-n593kh7u 9 12 of of IN cord-343256-n593kh7u 9 13 antigen antigen NN cord-343256-n593kh7u 9 14 , , , cord-343256-n593kh7u 9 15 and and CC cord-343256-n593kh7u 9 16 sera sera NNP cord-343256-n593kh7u 9 17 from from IN cord-343256-n593kh7u 9 18 rats rat NNS cord-343256-n593kh7u 9 19 collected collect VBD cord-343256-n593kh7u 9 20 post post NN cord-343256-n593kh7u 9 21 inoculation inoculation NN cord-343256-n593kh7u 9 22 with with IN cord-343256-n593kh7u 9 23 either either DT cord-343256-n593kh7u 9 24 of of IN cord-343256-n593kh7u 9 25 these these DT cord-343256-n593kh7u 9 26 viral viral JJ cord-343256-n593kh7u 9 27 strains strain NNS cord-343256-n593kh7u 9 28 , , , cord-343256-n593kh7u 9 29 the the DT cord-343256-n593kh7u 9 30 indirect indirect JJ cord-343256-n593kh7u 9 31 fluorescent fluorescent NN cord-343256-n593kh7u 9 32 antibody antibody NN cord-343256-n593kh7u 9 33 ( ( -LRB- cord-343256-n593kh7u 9 34 IFA IFA NNP cord-343256-n593kh7u 9 35 ) ) -RRB- cord-343256-n593kh7u 9 36 procedure procedure NN cord-343256-n593kh7u 9 37 was be VBD cord-343256-n593kh7u 9 38 used use VBN cord-343256-n593kh7u 9 39 to to TO cord-343256-n593kh7u 9 40 determine determine VB cord-343256-n593kh7u 9 41 whether whether IN cord-343256-n593kh7u 9 42 antibody antibody NN cord-343256-n593kh7u 9 43 titers titer NNS cord-343256-n593kh7u 9 44 could could MD cord-343256-n593kh7u 9 45 be be VB cord-343256-n593kh7u 9 46 used use VBN cord-343256-n593kh7u 9 47 to to TO cord-343256-n593kh7u 9 48 differentiate differentiate VB cord-343256-n593kh7u 9 49 infections infection NNS cord-343256-n593kh7u 9 50 from from IN cord-343256-n593kh7u 9 51 the the DT cord-343256-n593kh7u 9 52 homologous homologous JJ cord-343256-n593kh7u 9 53 and and CC cord-343256-n593kh7u 9 54 heterologous heterologous JJ cord-343256-n593kh7u 9 55 virus virus NN cord-343256-n593kh7u 9 56 . . . cord-343256-n593kh7u 10 1 There there EX cord-343256-n593kh7u 10 2 was be VBD cord-343256-n593kh7u 10 3 no no DT cord-343256-n593kh7u 10 4 detectable detectable JJ cord-343256-n593kh7u 10 5 difference difference NN cord-343256-n593kh7u 10 6 in in IN cord-343256-n593kh7u 10 7 the the DT cord-343256-n593kh7u 10 8 sensitivity sensitivity NN cord-343256-n593kh7u 10 9 or or CC cord-343256-n593kh7u 10 10 specificity specificity NN cord-343256-n593kh7u 10 11 of of IN cord-343256-n593kh7u 10 12 these these DT cord-343256-n593kh7u 10 13 systems system NNS cord-343256-n593kh7u 10 14 in in IN cord-343256-n593kh7u 10 15 detecting detect VBG cord-343256-n593kh7u 10 16 antibody antibody NN cord-343256-n593kh7u 10 17 to to IN cord-343256-n593kh7u 10 18 the the DT cord-343256-n593kh7u 10 19 homologous homologous JJ cord-343256-n593kh7u 10 20 or or CC cord-343256-n593kh7u 10 21 heterologous heterologous JJ cord-343256-n593kh7u 10 22 virus virus NN cord-343256-n593kh7u 10 23 . . . cord-343256-n593kh7u 11 1 Thus thus RB cord-343256-n593kh7u 11 2 there there EX cord-343256-n593kh7u 11 3 was be VBD cord-343256-n593kh7u 11 4 no no DT cord-343256-n593kh7u 11 5 evidence evidence NN cord-343256-n593kh7u 11 6 that that IN cord-343256-n593kh7u 11 7 SDAV- sdav- JJ cord-343256-n593kh7u 11 8 and and CC cord-343256-n593kh7u 11 9 PRC PRC NNP cord-343256-n593kh7u 11 10 - - HYPH cord-343256-n593kh7u 11 11 infected infect VBN cord-343256-n593kh7u 11 12 cells cell NNS cord-343256-n593kh7u 11 13 would would MD cord-343256-n593kh7u 11 14 serve serve VB cord-343256-n593kh7u 11 15 to to TO cord-343256-n593kh7u 11 16 differentiate differentiate VB cord-343256-n593kh7u 11 17 antibody antibody NN cord-343256-n593kh7u 11 18 to to IN cord-343256-n593kh7u 11 19 the the DT cord-343256-n593kh7u 11 20 homologous homologous JJ cord-343256-n593kh7u 11 21 virus virus NN cord-343256-n593kh7u 11 22 using use VBG cord-343256-n593kh7u 11 23 the the DT cord-343256-n593kh7u 11 24 IFA IFA NNP cord-343256-n593kh7u 11 25 technique technique NN cord-343256-n593kh7u 11 26 . . . cord-343256-n593kh7u 12 1 In in IN cord-343256-n593kh7u 12 2 addition addition NN cord-343256-n593kh7u 12 3 , , , cord-343256-n593kh7u 12 4 antibody antibody NN cord-343256-n593kh7u 12 5 titers titer NNS cord-343256-n593kh7u 12 6 were be VBD cord-343256-n593kh7u 12 7 similar similar JJ cord-343256-n593kh7u 12 8 when when WRB cord-343256-n593kh7u 12 9 mouse mouse NNP cord-343256-n593kh7u 12 10 hepatitis hepatitis NN cord-343256-n593kh7u 12 11 virus virus NN cord-343256-n593kh7u 12 12 ( ( -LRB- cord-343256-n593kh7u 12 13 MHV)-infected mhv)-infected JJ cord-343256-n593kh7u 12 14 cells cell NNS cord-343256-n593kh7u 12 15 were be VBD cord-343256-n593kh7u 12 16 used use VBN cord-343256-n593kh7u 12 17 as as IN cord-343256-n593kh7u 12 18 the the DT cord-343256-n593kh7u 12 19 source source NN cord-343256-n593kh7u 12 20 of of IN cord-343256-n593kh7u 12 21 antigen antigen NN cord-343256-n593kh7u 12 22 for for IN cord-343256-n593kh7u 12 23 the the DT cord-343256-n593kh7u 12 24 IFA IFA NNP cord-343256-n593kh7u 12 25 technique technique NN cord-343256-n593kh7u 12 26 . . . cord-343256-n593kh7u 13 1 However however RB cord-343256-n593kh7u 13 2 , , , cord-343256-n593kh7u 13 3 using use VBG cord-343256-n593kh7u 13 4 MHV MHV NNP cord-343256-n593kh7u 13 5 or or CC cord-343256-n593kh7u 13 6 SDAV SDAV NNP cord-343256-n593kh7u 13 7 - - HYPH cord-343256-n593kh7u 13 8 infected infect VBN cord-343256-n593kh7u 13 9 cells cell NNS cord-343256-n593kh7u 13 10 as as IN cord-343256-n593kh7u 13 11 the the DT cord-343256-n593kh7u 13 12 source source NN cord-343256-n593kh7u 13 13 of of IN cord-343256-n593kh7u 13 14 antigen antigen NN cord-343256-n593kh7u 13 15 , , , cord-343256-n593kh7u 13 16 there there EX cord-343256-n593kh7u 13 17 was be VBD cord-343256-n593kh7u 13 18 a a DT cord-343256-n593kh7u 13 19 significant significant JJ cord-343256-n593kh7u 13 20 difference difference NN cord-343256-n593kh7u 13 21 in in IN cord-343256-n593kh7u 13 22 antibody antibody NN cord-343256-n593kh7u 13 23 titers titer NNS cord-343256-n593kh7u 13 24 to to IN cord-343256-n593kh7u 13 25 the the DT cord-343256-n593kh7u 13 26 homologous homologous JJ cord-343256-n593kh7u 13 27 virus virus NN cord-343256-n593kh7u 13 28 detected detect VBD cord-343256-n593kh7u 13 29 using use VBG cord-343256-n593kh7u 13 30 the the DT cord-343256-n593kh7u 13 31 immunoenzyme immunoenzyme NNP cord-343256-n593kh7u 13 32 technique technique NN cord-343256-n593kh7u 13 33 . . . cord-343256-n593kh7u 14 1 The the DT cord-343256-n593kh7u 14 2 indirect indirect JJ cord-343256-n593kh7u 14 3 fluorescence fluorescence NN cord-343256-n593kh7u 14 4 antibody antibody NN cord-343256-n593kh7u 14 5 ( ( -LRB- cord-343256-n593kh7u 14 6 IFA IFA NNP cord-343256-n593kh7u 14 7 ) ) -RRB- cord-343256-n593kh7u 14 8 test test NN cord-343256-n593kh7u 14 9 is be VBZ cord-343256-n593kh7u 14 10 a a DT cord-343256-n593kh7u 14 11 recognized recognize VBN cord-343256-n593kh7u 14 12 , , , cord-343256-n593kh7u 14 13 sensitive sensitive JJ cord-343256-n593kh7u 14 14 method method NN cord-343256-n593kh7u 14 15 to to TO cord-343256-n593kh7u 14 16 detect detect VB cord-343256-n593kh7u 14 17 antibody antibody NN cord-343256-n593kh7u 14 18 to to IN cord-343256-n593kh7u 14 19 specific specific JJ cord-343256-n593kh7u 14 20 rodent rodent NN cord-343256-n593kh7u 14 21 viruses virus NNS cord-343256-n593kh7u 14 22 . . . cord-343256-n593kh7u 15 1 One one CD cord-343256-n593kh7u 15 2 method method NN cord-343256-n593kh7u 15 3 used use VBN cord-343256-n593kh7u 15 4 for for IN cord-343256-n593kh7u 15 5 the the DT cord-343256-n593kh7u 15 6 detection detection NN cord-343256-n593kh7u 15 7 of of IN cord-343256-n593kh7u 15 8 coronaviral coronaviral JJ cord-343256-n593kh7u 15 9 antibodies antibody NNS cord-343256-n593kh7u 15 10 in in IN cord-343256-n593kh7u 15 11 rodent rodent NN cord-343256-n593kh7u 15 12 sera sera NNP cord-343256-n593kh7u 15 13 consists consist VBZ cord-343256-n593kh7u 15 14 of of IN cord-343256-n593kh7u 15 15 equal equal JJ cord-343256-n593kh7u 15 16 proportions proportion NNS cord-343256-n593kh7u 15 17 of of IN cord-343256-n593kh7u 15 18 cells cell NNS cord-343256-n593kh7u 15 19 infected infect VBN cord-343256-n593kh7u 15 20 with with IN cord-343256-n593kh7u 15 21 the the DT cord-343256-n593kh7u 15 22 JHM JHM NNP cord-343256-n593kh7u 15 23 and and CC cord-343256-n593kh7u 15 24 S S NNP cord-343256-n593kh7u 15 25 strains strain NNS cord-343256-n593kh7u 15 26 of of IN cord-343256-n593kh7u 15 27 mouse mouse NN cord-343256-n593kh7u 15 28 hepatitis hepatitis NN cord-343256-n593kh7u 15 29 virus virus NN cord-343256-n593kh7u 15 30 ( ( -LRB- cord-343256-n593kh7u 15 31 MHV MHV NNP cord-343256-n593kh7u 15 32 ) ) -RRB- cord-343256-n593kh7u 15 33 as as IN cord-343256-n593kh7u 15 34 a a DT cord-343256-n593kh7u 15 35 source source NN cord-343256-n593kh7u 15 36 of of IN cord-343256-n593kh7u 15 37 antigen antigen NN cord-343256-n593kh7u 15 38 , , , cord-343256-n593kh7u 15 39 together together RB cord-343256-n593kh7u 15 40 with with IN cord-343256-n593kh7u 15 41 uninfected uninfected JJ cord-343256-n593kh7u 15 42 cells cell NNS cord-343256-n593kh7u 15 43 . . . cord-343256-n593kh7u 16 1 Using use VBG cord-343256-n593kh7u 16 2 this this DT cord-343256-n593kh7u 16 3 as as IN cord-343256-n593kh7u 16 4 a a DT cord-343256-n593kh7u 16 5 source source NN cord-343256-n593kh7u 16 6 of of IN cord-343256-n593kh7u 16 7 antigen antigen NN cord-343256-n593kh7u 16 8 , , , cord-343256-n593kh7u 16 9 the the DT cord-343256-n593kh7u 16 10 indirect indirect JJ cord-343256-n593kh7u 16 11 fluorescent fluorescent JJ cord-343256-n593kh7u 16 12 antibody antibody NN cord-343256-n593kh7u 16 13 procedure procedure NN cord-343256-n593kh7u 16 14 has have VBZ cord-343256-n593kh7u 16 15 proven prove VBN cord-343256-n593kh7u 16 16 to to TO cord-343256-n593kh7u 16 17 be be VB cord-343256-n593kh7u 16 18 a a DT cord-343256-n593kh7u 16 19 test test NN cord-343256-n593kh7u 16 20 system system NN cord-343256-n593kh7u 16 21 with with IN cord-343256-n593kh7u 16 22 D.H. D.H. NNP cord-343256-n593kh7u 16 23 Percy Percy NNP cord-343256-n593kh7u 16 24 et et NNP cord-343256-n593kh7u 16 25 al al NNP cord-343256-n593kh7u 16 26 . . . cord-343256-n593kh7u 17 1 good good JJ cord-343256-n593kh7u 17 2 sensitivity sensitivity NN cord-343256-n593kh7u 17 3 for for IN cord-343256-n593kh7u 17 4 the the DT cord-343256-n593kh7u 17 5 detection detection NN cord-343256-n593kh7u 17 6 of of IN cord-343256-n593kh7u 17 7 animals animal NNS cord-343256-n593kh7u 17 8 seropositive seropositive JJ cord-343256-n593kh7u 17 9 for for IN cord-343256-n593kh7u 17 10 routine routine JJ cord-343256-n593kh7u 17 11 coronaviruses coronaviruse NNS cord-343256-n593kh7u 17 12 [ [ -LRB- cord-343256-n593kh7u 17 13 6 6 CD cord-343256-n593kh7u 17 14 ] ] -RRB- cord-343256-n593kh7u 17 15 . . . cord-343256-n593kh7u 18 1 Similarly similarly RB cord-343256-n593kh7u 18 2 , , , cord-343256-n593kh7u 18 3 enzyme enzyme NN cord-343256-n593kh7u 19 1 i i PRP cord-343256-n593kh7u 20 1 m m NNP cord-343256-n593kh7u 20 2 m m NNP cord-343256-n593kh7u 21 1 u u NNP cord-343256-n593kh7u 21 2 n n NN cord-343256-n593kh7u 21 3 o o NN cord-343256-n593kh7u 22 1 a a DT cord-343256-n593kh7u 22 2 s s NNP cord-343256-n593kh7u 22 3 s s NN cord-343256-n593kh7u 22 4 a a DT cord-343256-n593kh7u 22 5 y y NN cord-343256-n593kh7u 22 6 ( ( -LRB- cord-343256-n593kh7u 22 7 EIA EIA NNP cord-343256-n593kh7u 22 8 ) ) -RRB- cord-343256-n593kh7u 22 9 procedures procedure NNS cord-343256-n593kh7u 22 10 have have VBP cord-343256-n593kh7u 22 11 been be VBN cord-343256-n593kh7u 22 12 developed develop VBN cord-343256-n593kh7u 22 13 for for IN cord-343256-n593kh7u 22 14 antibody antibody NN cord-343256-n593kh7u 22 15 detection detection NN cord-343256-n593kh7u 22 16 and and CC cord-343256-n593kh7u 22 17 titration titration NN cord-343256-n593kh7u 22 18 . . . cord-343256-n593kh7u 23 1 Using use VBG cord-343256-n593kh7u 23 2 the the DT cord-343256-n593kh7u 23 3 horseradish horseradish NN cord-343256-n593kh7u 23 4 peroxidase peroxidase NN cord-343256-n593kh7u 23 5 or or CC cord-343256-n593kh7u 23 6 the the DT cord-343256-n593kh7u 23 7 urease urease NN cord-343256-n593kh7u 23 8 assay assay NN cord-343256-n593kh7u 23 9 , , , cord-343256-n593kh7u 23 10 antibody antibody NN cord-343256-n593kh7u 23 11 titers titer NNS cord-343256-n593kh7u 24 1 m m LS cord-343256-n593kh7u 25 1 a a DT cord-343256-n593kh7u 25 2 y y NN cord-343256-n593kh7u 25 3 be be VB cord-343256-n593kh7u 25 4 substantially substantially RB cord-343256-n593kh7u 25 5 higher high JJR cord-343256-n593kh7u 25 6 than than IN cord-343256-n593kh7u 25 7 those those DT cord-343256-n593kh7u 25 8 detected detect VBN cord-343256-n593kh7u 25 9 with with IN cord-343256-n593kh7u 25 10 the the DT cord-343256-n593kh7u 25 11 I I NNP cord-343256-n593kh7u 25 12 F F NNP cord-343256-n593kh7u 25 13 A A NNP cord-343256-n593kh7u 25 14 technique technique NN cord-343256-n593kh7u 25 15 [ [ -LRB- cord-343256-n593kh7u 25 16 7 7 CD cord-343256-n593kh7u 25 17 ] ] -RRB- cord-343256-n593kh7u 25 18 . . . cord-343256-n593kh7u 26 1 Using use VBG cord-343256-n593kh7u 26 2 the the DT cord-343256-n593kh7u 26 3 L-2 L-2 NNP cord-343256-n593kh7u 26 4 subline subline NN cord-343256-n593kh7u 26 5 of of IN cord-343256-n593kh7u 26 6 L-929 l-929 NN cord-343256-n593kh7u 26 7 cells cell NNS cord-343256-n593kh7u 26 8 , , , cord-343256-n593kh7u 26 9 we -PRON- PRP cord-343256-n593kh7u 26 10 have have VBP cord-343256-n593kh7u 26 11 been be VBN cord-343256-n593kh7u 26 12 able able JJ cord-343256-n593kh7u 26 13 to to TO cord-343256-n593kh7u 26 14 replicate replicate VB cord-343256-n593kh7u 26 15 relatively relatively RB cord-343256-n593kh7u 26 16 high high JJ cord-343256-n593kh7u 26 17 titers titer NNS cord-343256-n593kh7u 26 18 of of IN cord-343256-n593kh7u 26 19 sialodacryoadenitis sialodacryoadenitis NNP cord-343256-n593kh7u 26 20 virus virus NN cord-343256-n593kh7u 26 21 ( ( -LRB- cord-343256-n593kh7u 26 22 SDAV SDAV NNP cord-343256-n593kh7u 26 23 ) ) -RRB- cord-343256-n593kh7u 26 24 [ [ -LRB- cord-343256-n593kh7u 26 25 4 4 CD cord-343256-n593kh7u 26 26 ] ] -RRB- cord-343256-n593kh7u 26 27 , , , cord-343256-n593kh7u 26 28 and and CC cord-343256-n593kh7u 26 29 Parker Parker NNP cord-343256-n593kh7u 26 30 's 's POS cord-343256-n593kh7u 26 31 rat rat NN cord-343256-n593kh7u 26 32 coronavirus coronavirus NN cord-343256-n593kh7u 26 33 ( ( -LRB- cord-343256-n593kh7u 26 34 PRC PRC NNP cord-343256-n593kh7u 26 35 ) ) -RRB- cord-343256-n593kh7u 26 36 [ [ -LRB- cord-343256-n593kh7u 26 37 5 5 LS cord-343256-n593kh7u 26 38 ] ] -RRB- cord-343256-n593kh7u 26 39 in in IN cord-343256-n593kh7u 26 40 vitro vitro FW cord-343256-n593kh7u 26 41 . . . cord-343256-n593kh7u 27 1 The the DT cord-343256-n593kh7u 27 2 purpose purpose NN cord-343256-n593kh7u 27 3 of of IN cord-343256-n593kh7u 27 4 this this DT cord-343256-n593kh7u 27 5 study study NN cord-343256-n593kh7u 27 6 was be VBD cord-343256-n593kh7u 27 7 to to TO cord-343256-n593kh7u 27 8 compare compare VB cord-343256-n593kh7u 27 9 the the DT cord-343256-n593kh7u 27 10 sensitivity sensitivity NN cord-343256-n593kh7u 27 11 of of IN cord-343256-n593kh7u 27 12 SDAV SDAV NNP cord-343256-n593kh7u 27 13 or or CC cord-343256-n593kh7u 27 14 PRC PRC NNP cord-343256-n593kh7u 27 15 - - HYPH cord-343256-n593kh7u 27 16 infected infect VBN cord-343256-n593kh7u 27 17 L-2 L-2 NNP cord-343256-n593kh7u 27 18 cells cell NNS cord-343256-n593kh7u 27 19 with with IN cord-343256-n593kh7u 27 20 MHV mhv NN cord-343256-n593kh7u 27 21 - - HYPH cord-343256-n593kh7u 27 22 infected infect VBN cord-343256-n593kh7u 27 23 cells cell NNS cord-343256-n593kh7u 27 24 for for IN cord-343256-n593kh7u 27 25 the the DT cord-343256-n593kh7u 27 26 detection detection NN cord-343256-n593kh7u 27 27 and and CC cord-343256-n593kh7u 27 28 titration titration NN cord-343256-n593kh7u 27 29 of of IN cord-343256-n593kh7u 27 30 antibody antibody NN cord-343256-n593kh7u 27 31 to to IN cord-343256-n593kh7u 27 32 S S NNP cord-343256-n593kh7u 27 33 D D NNP cord-343256-n593kh7u 27 34 A a NN cord-343256-n593kh7u 27 35 V v NN cord-343256-n593kh7u 27 36 or or CC cord-343256-n593kh7u 27 37 PRC PRC NNP cord-343256-n593kh7u 27 38 . . . cord-343256-n593kh7u 28 1 The the DT cord-343256-n593kh7u 28 2 techniques technique NNS cord-343256-n593kh7u 28 3 used use VBN cord-343256-n593kh7u 28 4 were be VBD cord-343256-n593kh7u 28 5 I -PRON- PRP cord-343256-n593kh7u 28 6 F F NNP cord-343256-n593kh7u 28 7 A A NNP cord-343256-n593kh7u 28 8 and and CC cord-343256-n593kh7u 28 9 an an DT cord-343256-n593kh7u 28 10 i i NN cord-343256-n593kh7u 28 11 m m NN cord-343256-n593kh7u 28 12 m m NN cord-343256-n593kh7u 28 13 u u NNP cord-343256-n593kh7u 28 14 n n NN cord-343256-n593kh7u 28 15 o o NN cord-343256-n593kh7u 28 16 e e NN cord-343256-n593kh7u 28 17 n n NNP cord-343256-n593kh7u 28 18 z z NNP cord-343256-n593kh7u 28 19 y y NNP cord-343256-n593kh7u 28 20 m m NNP cord-343256-n593kh7u 28 21 e e NNP cord-343256-n593kh7u 28 22 procedure procedure NN cord-343256-n593kh7u 28 23 . . . cord-343256-n593kh7u 29 1 The the DT cord-343256-n593kh7u 29 2 L-2 L-2 NNP cord-343256-n593kh7u 29 3 subline subline NN cord-343256-n593kh7u 29 4 of of IN cord-343256-n593kh7u 29 5 L-929 l-929 NN cord-343256-n593kh7u 29 6 cells cell NNS cord-343256-n593kh7u 29 7 for for IN cord-343256-n593kh7u 29 8 the the DT cord-343256-n593kh7u 29 9 replication replication NN cord-343256-n593kh7u 29 10 of of IN cord-343256-n593kh7u 29 11 SDAV SDAV NNP cord-343256-n593kh7u 29 12 or or CC cord-343256-n593kh7u 29 13 PRC PRC NNP cord-343256-n593kh7u 29 14 was be VBD cord-343256-n593kh7u 29 15 obtained obtain VBN cord-343256-n593kh7u 29 16 from from IN cord-343256-n593kh7u 29 17 Dr. Dr. NNP cord-343256-n593kh7u 29 18 V. V. NNP cord-343256-n593kh7u 29 19 L. L. NNP cord-343256-n593kh7u 29 20 Morris Morris NNP cord-343256-n593kh7u 29 21 , , , cord-343256-n593kh7u 29 22 University University NNP cord-343256-n593kh7u 29 23 of of IN cord-343256-n593kh7u 29 24 Western Western NNP cord-343256-n593kh7u 29 25 Ontario Ontario NNP cord-343256-n593kh7u 29 26 , , , cord-343256-n593kh7u 29 27 London London NNP cord-343256-n593kh7u 29 28 , , , cord-343256-n593kh7u 29 29 Ontario Ontario NNP cord-343256-n593kh7u 29 30 . . . cord-343256-n593kh7u 30 1 MHV mhv NN cord-343256-n593kh7u 30 2 - - HYPH cord-343256-n593kh7u 30 3 infected infect VBN cord-343256-n593kh7u 30 4 NCTC NCTC NNP cord-343256-n593kh7u 30 5 1469 1469 CD cord-343256-n593kh7u 30 6 cells cell NNS cord-343256-n593kh7u 30 7 , , , cord-343256-n593kh7u 30 8 prepared prepare VBD cord-343256-n593kh7u 30 9 as as IN cord-343256-n593kh7u 30 10 previously previously RB cord-343256-n593kh7u 30 11 described describe VBN cord-343256-n593kh7u 30 12 for for IN cord-343256-n593kh7u 30 13 L-929 l-929 NN cord-343256-n593kh7u 30 14 cells cell NNS cord-343256-n593kh7u 30 15 [ [ -LRB- cord-343256-n593kh7u 30 16 6 6 CD cord-343256-n593kh7u 30 17 ] ] -RRB- cord-343256-n593kh7u 30 18 were be VBD cord-343256-n593kh7u 30 19 acquired acquire VBN cord-343256-n593kh7u 30 20 from from IN cord-343256-n593kh7u 30 21 Dr. Dr. NNP cord-343256-n593kh7u 30 22 A. A. NNP cord-343256-n593kh7u 30 23 L. L. NNP cord-343256-n593kh7u 30 24 Smith Smith NNP cord-343256-n593kh7u 30 25 , , , cord-343256-n593kh7u 30 26 Section Section NNP cord-343256-n593kh7u 30 27 of of IN cord-343256-n593kh7u 30 28 Comparative Comparative NNP cord-343256-n593kh7u 30 29 Medicine Medicine NNP cord-343256-n593kh7u 30 30 , , , cord-343256-n593kh7u 30 31 Yale Yale NNP cord-343256-n593kh7u 30 32 School School NNP cord-343256-n593kh7u 30 33 of of IN cord-343256-n593kh7u 30 34 Medicine Medicine NNP cord-343256-n593kh7u 30 35 . . . cord-343256-n593kh7u 31 1 The the DT cord-343256-n593kh7u 31 2 # # $ cord-343256-n593kh7u 31 3 681 681 CD cord-343256-n593kh7u 31 4 strain strain NN cord-343256-n593kh7u 31 5 of of IN cord-343256-n593kh7u 31 6 SDAV SDAV NNP cord-343256-n593kh7u 31 7 ( ( -LRB- cord-343256-n593kh7u 31 8 family family NN cord-343256-n593kh7u 31 9 Coronaviridae Coronaviridae NNP cord-343256-n593kh7u 31 10 ) ) -RRB- cord-343256-n593kh7u 31 11 was be VBD cord-343256-n593kh7u 31 12 obtained obtain VBN cord-343256-n593kh7u 31 13 from from IN cord-343256-n593kh7u 31 14 Dr. Dr. NNP cord-343256-n593kh7u 31 15 P. P. NNP cord-343256-n593kh7u 31 16 N. N. NNP cord-343256-n593kh7u 31 17 Bhatt Bhatt NNP cord-343256-n593kh7u 31 18 , , , cord-343256-n593kh7u 31 19 Yale Yale NNP cord-343256-n593kh7u 31 20 School School NNP cord-343256-n593kh7u 31 21 of of IN cord-343256-n593kh7u 31 22 Medicine Medicine NNP cord-343256-n593kh7u 31 23 . . . cord-343256-n593kh7u 32 1 The the DT cord-343256-n593kh7u 32 2 isolate isolate NN cord-343256-n593kh7u 32 3 of of IN cord-343256-n593kh7u 32 4 PRC PRC NNP cord-343256-n593kh7u 32 5 ( ( -LRB- cord-343256-n593kh7u 32 6 family family NN cord-343256-n593kh7u 32 7 Coronaviridae Coronaviridae NNP cord-343256-n593kh7u 32 8 ) ) -RRB- cord-343256-n593kh7u 32 9 was be VBD cord-343256-n593kh7u 32 10 obtained obtain VBN cord-343256-n593kh7u 32 11 from from IN cord-343256-n593kh7u 32 12 the the DT cord-343256-n593kh7u 32 13 American American NNP cord-343256-n593kh7u 32 14 Type Type NNP cord-343256-n593kh7u 32 15 Culture Culture NNP cord-343256-n593kh7u 32 16 Collection Collection NNP cord-343256-n593kh7u 32 17 , , , cord-343256-n593kh7u 32 18 Rockville Rockville NNP cord-343256-n593kh7u 32 19 , , , cord-343256-n593kh7u 32 20 MD MD NNP cord-343256-n593kh7u 32 21 . . . cord-343256-n593kh7u 33 1 SDAV SDAV NNP cord-343256-n593kh7u 33 2 and and CC cord-343256-n593kh7u 33 3 PRC PRC NNP cord-343256-n593kh7u 33 4 were be VBD cord-343256-n593kh7u 33 5 replicated replicate VBN cord-343256-n593kh7u 33 6 in in IN cord-343256-n593kh7u 33 7 L-2 L-2 NNP cord-343256-n593kh7u 33 8 cells cell NNS cord-343256-n593kh7u 33 9 as as IN cord-343256-n593kh7u 33 10 previously previously RB cord-343256-n593kh7u 33 11 described describe VBN cord-343256-n593kh7u 33 12 [ [ -LRB- cord-343256-n593kh7u 33 13 4 4 CD cord-343256-n593kh7u 33 14 , , , cord-343256-n593kh7u 33 15 5 5 CD cord-343256-n593kh7u 33 16 ] ] -RRB- cord-343256-n593kh7u 33 17 . . . cord-343256-n593kh7u 34 1 For for IN cord-343256-n593kh7u 34 2 the the DT cord-343256-n593kh7u 34 3 preparation preparation NN cord-343256-n593kh7u 34 4 of of IN cord-343256-n593kh7u 34 5 viral viral JJ cord-343256-n593kh7u 34 6 antigen antigen NN cord-343256-n593kh7u 34 7 from from IN cord-343256-n593kh7u 34 8 rat rat NN cord-343256-n593kh7u 34 9 coronaviruses coronaviruse NNS cord-343256-n593kh7u 34 10 , , , cord-343256-n593kh7u 34 11 L-2 L-2 NNP cord-343256-n593kh7u 34 12 cells cell NNS cord-343256-n593kh7u 34 13 were be VBD cord-343256-n593kh7u 34 14 propagated propagate VBN cord-343256-n593kh7u 34 15 in in IN cord-343256-n593kh7u 34 16 100 100 CD cord-343256-n593kh7u 34 17 x x SYM cord-343256-n593kh7u 34 18 15 15 CD cord-343256-n593kh7u 34 19 mm mm NN cord-343256-n593kh7u 34 20 Nunc Nunc NNP cord-343256-n593kh7u 34 21 polystyrene polystyrene NN cord-343256-n593kh7u 34 22 tissue tissue NN cord-343256-n593kh7u 34 23 culture culture NN cord-343256-n593kh7u 34 24 dishes dish NNS cord-343256-n593kh7u 35 1 ( ( -LRB- cord-343256-n593kh7u 35 2 Gibco Gibco NNP cord-343256-n593kh7u 35 3 / / SYM cord-343256-n593kh7u 35 4 BRL BRL NNP cord-343256-n593kh7u 35 5 Inc Inc NNP cord-343256-n593kh7u 35 6 , , , cord-343256-n593kh7u 35 7 Burlington Burlington NNP cord-343256-n593kh7u 35 8 , , , cord-343256-n593kh7u 35 9 Ont Ont NNP cord-343256-n593kh7u 35 10 . . . cord-343256-n593kh7u 35 11 ) ) -RRB- cord-343256-n593kh7u 36 1 in in IN cord-343256-n593kh7u 36 2 Eagle Eagle NNP cord-343256-n593kh7u 36 3 's 's POS cord-343256-n593kh7u 36 4 minimal minimal JJ cord-343256-n593kh7u 36 5 essential essential JJ cord-343256-n593kh7u 36 6 medium medium NN cord-343256-n593kh7u 36 7 ( ( -LRB- cord-343256-n593kh7u 36 8 Gibco Gibco NNP cord-343256-n593kh7u 36 9 / / SYM cord-343256-n593kh7u 36 10 BRL BRL NNP cord-343256-n593kh7u 36 11 Inc Inc NNP cord-343256-n593kh7u 36 12 ) ) -RRB- cord-343256-n593kh7u 36 13 . . . cord-343256-n593kh7u 37 1 Cells cell NNS cord-343256-n593kh7u 37 2 were be VBD cord-343256-n593kh7u 37 3 grown grow VBN cord-343256-n593kh7u 37 4 in in IN cord-343256-n593kh7u 37 5 a a DT cord-343256-n593kh7u 37 6 humidified humidified JJ cord-343256-n593kh7u 37 7 atmosphere atmosphere NN cord-343256-n593kh7u 37 8 containing contain VBG cord-343256-n593kh7u 37 9 5 5 CD cord-343256-n593kh7u 37 10 % % NN cord-343256-n593kh7u 37 11 CO2 co2 NN cord-343256-n593kh7u 37 12 . . . cord-343256-n593kh7u 38 1 When when WRB cord-343256-n593kh7u 38 2 approximately approximately RB cord-343256-n593kh7u 38 3 80 80 CD cord-343256-n593kh7u 38 4 % % NN cord-343256-n593kh7u 38 5 confluent confluent JJ cord-343256-n593kh7u 38 6 , , , cord-343256-n593kh7u 38 7 monolayers monolayer NNS cord-343256-n593kh7u 38 8 were be VBD cord-343256-n593kh7u 38 9 inoculated inoculate VBN cord-343256-n593kh7u 38 10 with with IN cord-343256-n593kh7u 38 11 stock stock NN cord-343256-n593kh7u 38 12 virus virus NN cord-343256-n593kh7u 38 13 suspension suspension NN cord-343256-n593kh7u 38 14 , , , cord-343256-n593kh7u 38 15 and and CC cord-343256-n593kh7u 38 16 incubated incubate VBN cord-343256-n593kh7u 38 17 for for IN cord-343256-n593kh7u 38 18 48 48 CD cord-343256-n593kh7u 38 19 h h NN cord-343256-n593kh7u 38 20 at at IN cord-343256-n593kh7u 38 21 37 37 CD cord-343256-n593kh7u 38 22 ° ° NN cord-343256-n593kh7u 38 23 C c NN cord-343256-n593kh7u 38 24 in in IN cord-343256-n593kh7u 38 25 5 5 CD cord-343256-n593kh7u 38 26 % % NN cord-343256-n593kh7u 38 27 CO2 co2 NN cord-343256-n593kh7u 38 28 . . . cord-343256-n593kh7u 39 1 Infected infected JJ cord-343256-n593kh7u 39 2 and and CC cord-343256-n593kh7u 39 3 control control NN cord-343256-n593kh7u 39 4 cells cell NNS cord-343256-n593kh7u 39 5 were be VBD cord-343256-n593kh7u 39 6 then then RB cord-343256-n593kh7u 39 7 removed remove VBN cord-343256-n593kh7u 39 8 using use VBG cord-343256-n593kh7u 39 9 a a DT cord-343256-n593kh7u 39 10 rubber rubber NN cord-343256-n593kh7u 39 11 policeman policeman NN cord-343256-n593kh7u 39 12 , , , cord-343256-n593kh7u 39 13 placed place VBN cord-343256-n593kh7u 39 14 in in IN cord-343256-n593kh7u 39 15 two two CD cord-343256-n593kh7u 39 16 separate separate JJ cord-343256-n593kh7u 39 17 centrifuge centrifuge NN cord-343256-n593kh7u 39 18 tubes tube NNS cord-343256-n593kh7u 39 19 , , , cord-343256-n593kh7u 39 20 and and CC cord-343256-n593kh7u 39 21 centrifuged centrifuge VBN cord-343256-n593kh7u 39 22 at at IN cord-343256-n593kh7u 39 23 1600rpm 1600rpm CD cord-343256-n593kh7u 39 24 , , , cord-343256-n593kh7u 39 25 then then RB cord-343256-n593kh7u 39 26 resuspended resuspend VBD cord-343256-n593kh7u 39 27 in in IN cord-343256-n593kh7u 39 28 PBS PBS NNP cord-343256-n593kh7u 39 29 . . . cord-343256-n593kh7u 40 1 Cells cell NNS cord-343256-n593kh7u 40 2 were be VBD cord-343256-n593kh7u 40 3 then then RB cord-343256-n593kh7u 40 4 mixed mix VBN cord-343256-n593kh7u 40 5 at at IN cord-343256-n593kh7u 40 6 one one CD cord-343256-n593kh7u 40 7 part part NN cord-343256-n593kh7u 40 8 of of IN cord-343256-n593kh7u 40 9 infected infect VBN cord-343256-n593kh7u 40 10 to to IN cord-343256-n593kh7u 40 11 1.5 1.5 CD cord-343256-n593kh7u 40 12 parts part NNS cord-343256-n593kh7u 40 13 of of IN cord-343256-n593kh7u 40 14 noninfected noninfected JJ cord-343256-n593kh7u 40 15 cells cell NNS cord-343256-n593kh7u 40 16 . . . cord-343256-n593kh7u 41 1 20 20 CD cord-343256-n593kh7u 41 2 gl gl NNS cord-343256-n593kh7u 41 3 of of IN cord-343256-n593kh7u 41 4 the the DT cord-343256-n593kh7u 41 5 mixture mixture NN cord-343256-n593kh7u 41 6 were be VBD cord-343256-n593kh7u 41 7 then then RB cord-343256-n593kh7u 41 8 dropped drop VBN cord-343256-n593kh7u 41 9 on on IN cord-343256-n593kh7u 41 10 each each DT cord-343256-n593kh7u 41 11 well well NN cord-343256-n593kh7u 41 12 in in IN cord-343256-n593kh7u 41 13 multiwell multiwell NNP cord-343256-n593kh7u 41 14 depression depression NNP cord-343256-n593kh7u 41 15 glass glass NNP cord-343256-n593kh7u 41 16 slides slide NNS cord-343256-n593kh7u 42 1 ( ( -LRB- cord-343256-n593kh7u 42 2 Gibco Gibco NNP cord-343256-n593kh7u 42 3 / / SYM cord-343256-n593kh7u 42 4 BRL BRL NNP cord-343256-n593kh7u 42 5 Inc Inc NNP cord-343256-n593kh7u 42 6 ) ) -RRB- cord-343256-n593kh7u 42 7 . . . cord-343256-n593kh7u 43 1 Slides slide NNS cord-343256-n593kh7u 43 2 were be VBD cord-343256-n593kh7u 43 3 air air NN cord-343256-n593kh7u 43 4 dried dry VBN cord-343256-n593kh7u 43 5 for for IN cord-343256-n593kh7u 43 6 1 1 CD cord-343256-n593kh7u 43 7 h h NN cord-343256-n593kh7u 43 8 , , , cord-343256-n593kh7u 43 9 fixed fix VBN cord-343256-n593kh7u 43 10 in in IN cord-343256-n593kh7u 43 11 acetone acetone NN cord-343256-n593kh7u 43 12 , , , cord-343256-n593kh7u 43 13 and and CC cord-343256-n593kh7u 43 14 stored store VBN cord-343256-n593kh7u 43 15 at at IN cord-343256-n593kh7u 43 16 -7 -7 CD cord-343256-n593kh7u 43 17 0 0 CD cord-343256-n593kh7u 43 18 ° ° NN cord-343256-n593kh7u 43 19 C c NN cord-343256-n593kh7u 43 20 until until IN cord-343256-n593kh7u 43 21 tested test VBN cord-343256-n593kh7u 43 22 . . . cord-343256-n593kh7u 44 1 SPF SPF NNP cord-343256-n593kh7u 44 2 Wistar Wistar NNP cord-343256-n593kh7u 44 3 rats rat NNS cord-343256-n593kh7u 44 4 were be VBD cord-343256-n593kh7u 44 5 obtained obtain VBN cord-343256-n593kh7u 44 6 at at IN cord-343256-n593kh7u 44 7 approximately approximately RB cord-343256-n593kh7u 44 8 8 8 CD cord-343256-n593kh7u 44 9 weeks week NNS cord-343256-n593kh7u 44 10 of of IN cord-343256-n593kh7u 44 11 age age NN cord-343256-n593kh7u 44 12 from from IN cord-343256-n593kh7u 44 13 a a DT cord-343256-n593kh7u 44 14 commercial commercial JJ cord-343256-n593kh7u 44 15 supplier supplier NN cord-343256-n593kh7u 44 16 ( ( -LRB- cord-343256-n593kh7u 44 17 Charles Charles NNP cord-343256-n593kh7u 44 18 River River NNP cord-343256-n593kh7u 44 19 Laboratories Laboratories NNPS cord-343256-n593kh7u 44 20 , , , cord-343256-n593kh7u 44 21 St. St. NNP cord-343256-n593kh7u 44 22 Constant Constant NNP cord-343256-n593kh7u 44 23 , , , cord-343256-n593kh7u 44 24 Quebec Quebec NNP cord-343256-n593kh7u 44 25 ) ) -RRB- cord-343256-n593kh7u 44 26 . . . cord-343256-n593kh7u 45 1 Animals animal NNS cord-343256-n593kh7u 45 2 were be VBD cord-343256-n593kh7u 45 3 housed house VBN cord-343256-n593kh7u 45 4 in in IN cord-343256-n593kh7u 45 5 an an DT cord-343256-n593kh7u 45 6 isolation isolation NN cord-343256-n593kh7u 45 7 facility facility NN cord-343256-n593kh7u 45 8 throughout throughout IN cord-343256-n593kh7u 45 9 the the DT cord-343256-n593kh7u 45 10 inoculation inoculation NN cord-343256-n593kh7u 45 11 period period NN cord-343256-n593kh7u 45 12 . . . cord-343256-n593kh7u 46 1 In in IN cord-343256-n593kh7u 46 2 separate separate JJ cord-343256-n593kh7u 46 3 studies study NNS cord-343256-n593kh7u 46 4 and and CC cord-343256-n593kh7u 46 5 at at IN cord-343256-n593kh7u 46 6 different different JJ cord-343256-n593kh7u 46 7 time time NN cord-343256-n593kh7u 46 8 periods period NNS cord-343256-n593kh7u 46 9 , , , cord-343256-n593kh7u 46 10 rats rat NNS cord-343256-n593kh7u 46 11 were be VBD cord-343256-n593kh7u 46 12 inoculated inoculate VBN cord-343256-n593kh7u 46 13 intranasally intranasally RB cord-343256-n593kh7u 46 14 with with IN cord-343256-n593kh7u 46 15 approximately approximately RB cord-343256-n593kh7u 46 16 103 103 CD cord-343256-n593kh7u 46 17 TCIDs0 TCIDs0 NNP cord-343256-n593kh7u 46 18 of of IN cord-343256-n593kh7u 46 19 either either CC cord-343256-n593kh7u 46 20 the the DT cord-343256-n593kh7u 46 21 ATCC ATCC NNP cord-343256-n593kh7u 46 22 strain strain NN cord-343256-n593kh7u 46 23 of of IN cord-343256-n593kh7u 46 24 PRC PRC NNP cord-343256-n593kh7u 46 25 or or CC cord-343256-n593kh7u 46 26 strain strain VB cord-343256-n593kh7u 46 27 # # $ cord-343256-n593kh7u 46 28 681 681 CD cord-343256-n593kh7u 46 29 of of IN cord-343256-n593kh7u 46 30 SDAV SDAV NNP cord-343256-n593kh7u 47 1 ( ( -LRB- cord-343256-n593kh7u 47 2 second second JJ cord-343256-n593kh7u 47 3 passage passage NN cord-343256-n593kh7u 47 4 in in IN cord-343256-n593kh7u 47 5 L-2 L-2 NNP cord-343256-n593kh7u 47 6 cells cell NNS cord-343256-n593kh7u 47 7 ) ) -RRB- cord-343256-n593kh7u 47 8 . . . cord-343256-n593kh7u 48 1 Animals animal NNS cord-343256-n593kh7u 48 2 were be VBD cord-343256-n593kh7u 48 3 killed kill VBN cord-343256-n593kh7u 48 4 with with IN cord-343256-n593kh7u 48 5 an an DT cord-343256-n593kh7u 48 6 overdose overdose NN cord-343256-n593kh7u 48 7 of of IN cord-343256-n593kh7u 48 8 pentobarbitone pentobarbitone NN cord-343256-n593kh7u 48 9 sodium sodium NN cord-343256-n593kh7u 48 10 ( ( -LRB- cord-343256-n593kh7u 48 11 MTC MTC NNP cord-343256-n593kh7u 48 12 Pharmaceuticals Pharmaceuticals NNP cord-343256-n593kh7u 48 13 , , , cord-343256-n593kh7u 48 14 Mississauga Mississauga NNP cord-343256-n593kh7u 48 15 , , , cord-343256-n593kh7u 48 16 Ont Ont NNP cord-343256-n593kh7u 48 17 . . . cord-343256-n593kh7u 48 18 ) ) -RRB- cord-343256-n593kh7u 49 1 at at IN cord-343256-n593kh7u 49 2 6 6 CD cord-343256-n593kh7u 49 3 , , , cord-343256-n593kh7u 49 4 8 8 CD cord-343256-n593kh7u 49 5 , , , cord-343256-n593kh7u 49 6 14 14 CD cord-343256-n593kh7u 49 7 , , , cord-343256-n593kh7u 49 8 and and CC cord-343256-n593kh7u 49 9 21 21 CD cord-343256-n593kh7u 49 10 days day NNS cord-343256-n593kh7u 49 11 post post NN cord-343256-n593kh7u 49 12 - - NN cord-343256-n593kh7u 49 13 inoculation inoculation JJ cord-343256-n593kh7u 49 14 ( ( -LRB- cord-343256-n593kh7u 49 15 pi pi NNP cord-343256-n593kh7u 49 16 ) ) -RRB- cord-343256-n593kh7u 49 17 , , , cord-343256-n593kh7u 49 18 sera sera NNP cord-343256-n593kh7u 49 19 were be VBD cord-343256-n593kh7u 49 20 collected collect VBN cord-343256-n593kh7u 49 21 by by IN cord-343256-n593kh7u 49 22 cardiac cardiac JJ cord-343256-n593kh7u 49 23 puncture puncture NN cord-343256-n593kh7u 49 24 , , , cord-343256-n593kh7u 49 25 and and CC cord-343256-n593kh7u 49 26 tissues tissue NNS cord-343256-n593kh7u 49 27 were be VBD cord-343256-n593kh7u 49 28 collected collect VBN cord-343256-n593kh7u 49 29 from from IN cord-343256-n593kh7u 49 30 control control NN cord-343256-n593kh7u 49 31 and and CC cord-343256-n593kh7u 49 32 inoculated inoculate VBD cord-343256-n593kh7u 49 33 animals animal NNS cord-343256-n593kh7u 49 34 for for IN cord-343256-n593kh7u 49 35 histopathology histopathology NN cord-343256-n593kh7u 49 36 . . . cord-343256-n593kh7u 50 1 Sera Sera NNP cord-343256-n593kh7u 50 2 were be VBD cord-343256-n593kh7u 50 3 tested test VBN cord-343256-n593kh7u 50 4 for for IN cord-343256-n593kh7u 50 5 antibody antibody NN cord-343256-n593kh7u 50 6 titers titer NNS cord-343256-n593kh7u 50 7 using use VBG cord-343256-n593kh7u 50 8 the the DT cord-343256-n593kh7u 50 9 following follow VBG cord-343256-n593kh7u 50 10 virus virus NN cord-343256-n593kh7u 50 11 - - HYPH cord-343256-n593kh7u 50 12 infected infect VBN cord-343256-n593kh7u 50 13 cells cell NNS cord-343256-n593kh7u 50 14 as as IN cord-343256-n593kh7u 50 15 antigens antigen NNS cord-343256-n593kh7u 50 16 : : : cord-343256-n593kh7u 50 17 SDAV SDAV NNP cord-343256-n593kh7u 50 18 , , , cord-343256-n593kh7u 50 19 PRC PRC NNP cord-343256-n593kh7u 50 20 , , , cord-343256-n593kh7u 50 21 and and CC cord-343256-n593kh7u 50 22 MHV MHV NNP cord-343256-n593kh7u 50 23 . . . cord-343256-n593kh7u 51 1 Serum serum NN cord-343256-n593kh7u 51 2 samples sample NNS cord-343256-n593kh7u 51 3 were be VBD cord-343256-n593kh7u 51 4 diluted dilute VBN cord-343256-n593kh7u 51 5 in in IN cord-343256-n593kh7u 51 6 PBS PBS NNP cord-343256-n593kh7u 51 7 , , , cord-343256-n593kh7u 51 8 then then RB cord-343256-n593kh7u 51 9 individual individual JJ cord-343256-n593kh7u 51 10 wells well NNS cord-343256-n593kh7u 51 11 were be VBD cord-343256-n593kh7u 51 12 flooded flood VBN cord-343256-n593kh7u 51 13 with with IN cord-343256-n593kh7u 51 14 the the DT cord-343256-n593kh7u 51 15 appropriate appropriate JJ cord-343256-n593kh7u 51 16 dilution dilution NN cord-343256-n593kh7u 51 17 and and CC cord-343256-n593kh7u 51 18 processed process VBN cord-343256-n593kh7u 51 19 as as IN cord-343256-n593kh7u 51 20 previously previously RB cord-343256-n593kh7u 51 21 described describe VBN cord-343256-n593kh7u 51 22 [ [ -LRB- cord-343256-n593kh7u 51 23 6 6 CD cord-343256-n593kh7u 51 24 ] ] -RRB- cord-343256-n593kh7u 51 25 . . . cord-343256-n593kh7u 52 1 Fluorescein Fluorescein NNP cord-343256-n593kh7u 52 2 - - HYPH cord-343256-n593kh7u 52 3 labelled label VBN cord-343256-n593kh7u 52 4 anti anti JJ cord-343256-n593kh7u 52 5 - - JJ cord-343256-n593kh7u 52 6 rat rat JJ cord-343256-n593kh7u 52 7 globulin globulin NN cord-343256-n593kh7u 53 1 ( ( -LRB- cord-343256-n593kh7u 53 2 Antibodies Antibodies NNP cord-343256-n593kh7u 53 3 Inc. Inc. NNP cord-343256-n593kh7u 53 4 , , , cord-343256-n593kh7u 53 5 Davis Davis NNP cord-343256-n593kh7u 53 6 , , , cord-343256-n593kh7u 53 7 CA CA NNP cord-343256-n593kh7u 53 8 ) ) -RRB- cord-343256-n593kh7u 53 9 was be VBD cord-343256-n593kh7u 53 10 used use VBN cord-343256-n593kh7u 53 11 to to TO cord-343256-n593kh7u 53 12 identify identify VB cord-343256-n593kh7u 53 13 positive positive JJ cord-343256-n593kh7u 53 14 samples sample NNS cord-343256-n593kh7u 53 15 . . . cord-343256-n593kh7u 54 1 Evans Evans NNP cord-343256-n593kh7u 54 2 blue blue JJ cord-343256-n593kh7u 54 3 at at IN cord-343256-n593kh7u 54 4 0.005 0.005 CD cord-343256-n593kh7u 54 5 % % NN cord-343256-n593kh7u 54 6 was be VBD cord-343256-n593kh7u 54 7 used use VBN cord-343256-n593kh7u 54 8 as as IN cord-343256-n593kh7u 54 9 a a DT cord-343256-n593kh7u 54 10 counterstain counterstain NN cord-343256-n593kh7u 54 11 . . . cord-343256-n593kh7u 55 1 Specimens specimen NNS cord-343256-n593kh7u 55 2 were be VBD cord-343256-n593kh7u 55 3 examined examine VBN cord-343256-n593kh7u 55 4 using use VBG cord-343256-n593kh7u 55 5 a a DT cord-343256-n593kh7u 55 6 halogen halogen NN cord-343256-n593kh7u 55 7 - - HYPH cord-343256-n593kh7u 55 8 illuminated illuminate VBN cord-343256-n593kh7u 55 9 epifluorescence epifluorescence NN cord-343256-n593kh7u 55 10 microscope microscope NN cord-343256-n593kh7u 55 11 at at IN cord-343256-n593kh7u 55 12 x x SYM cord-343256-n593kh7u 55 13 250 250 CD cord-343256-n593kh7u 55 14 and and CC cord-343256-n593kh7u 55 15 x x SYM cord-343256-n593kh7u 55 16 400 400 CD cord-343256-n593kh7u 55 17 magnifications magnification NNS cord-343256-n593kh7u 55 18 . . . cord-343256-n593kh7u 56 1 All all DT cord-343256-n593kh7u 56 2 samples sample NNS cord-343256-n593kh7u 56 3 were be VBD cord-343256-n593kh7u 56 4 read read VBN cord-343256-n593kh7u 56 5 as as IN cord-343256-n593kh7u 56 6 unknowns unknown NNS cord-343256-n593kh7u 56 7 to to TO cord-343256-n593kh7u 56 8 avoid avoid VB cord-343256-n593kh7u 56 9 bias bias NN cord-343256-n593kh7u 56 10 . . . cord-343256-n593kh7u 57 1 Titers titer NNS cord-343256-n593kh7u 57 2 were be VBD cord-343256-n593kh7u 57 3 recorded record VBN cord-343256-n593kh7u 57 4 as as IN cord-343256-n593kh7u 57 5 the the DT cord-343256-n593kh7u 57 6 highest high JJS cord-343256-n593kh7u 57 7 dilution dilution NN cord-343256-n593kh7u 57 8 of of IN cord-343256-n593kh7u 57 9 the the DT cord-343256-n593kh7u 57 10 serum serum NN cord-343256-n593kh7u 57 11 sample sample NN cord-343256-n593kh7u 57 12 under under IN cord-343256-n593kh7u 57 13 test test NN cord-343256-n593kh7u 57 14 showing show VBG cord-343256-n593kh7u 57 15 fluorescence fluorescence NN cord-343256-n593kh7u 57 16 . . . cord-343256-n593kh7u 58 1 The the DT cord-343256-n593kh7u 58 2 differences difference NNS cord-343256-n593kh7u 58 3 between between IN cord-343256-n593kh7u 58 4 the the DT cord-343256-n593kh7u 58 5 mean mean JJ cord-343256-n593kh7u 58 6 values value NNS cord-343256-n593kh7u 58 7 for for IN cord-343256-n593kh7u 58 8 the the DT cord-343256-n593kh7u 58 9 antibody antibody NN cord-343256-n593kh7u 58 10 titers titer NNS cord-343256-n593kh7u 58 11 to to IN cord-343256-n593kh7u 58 12 the the DT cord-343256-n593kh7u 58 13 homologous homologous JJ cord-343256-n593kh7u 58 14 and and CC cord-343256-n593kh7u 58 15 heterologous heterologous JJ cord-343256-n593kh7u 58 16 viruses virus NNS cord-343256-n593kh7u 58 17 in in IN cord-343256-n593kh7u 58 18 each each DT cord-343256-n593kh7u 58 19 study study NN cord-343256-n593kh7u 58 20 were be VBD cord-343256-n593kh7u 58 21 tested test VBN cord-343256-n593kh7u 58 22 for for IN cord-343256-n593kh7u 58 23 significance significance NN cord-343256-n593kh7u 58 24 using use VBG cord-343256-n593kh7u 58 25 the the DT cord-343256-n593kh7u 58 26 general general JJ cord-343256-n593kh7u 58 27 linear linear JJ cord-343256-n593kh7u 58 28 model model NN cord-343256-n593kh7u 58 29 of of IN cord-343256-n593kh7u 58 30 SAS SAS NNP cord-343256-n593kh7u 58 31 ( ( -LRB- cord-343256-n593kh7u 58 32 GLM GLM NNP cord-343256-n593kh7u 58 33 ) ) -RRB- cord-343256-n593kh7u 58 34 . . . cord-343256-n593kh7u 59 1 For for IN cord-343256-n593kh7u 59 2 the the DT cord-343256-n593kh7u 59 3 immunoenzyme immunoenzyme NNP cord-343256-n593kh7u 59 4 technique technique NN cord-343256-n593kh7u 59 5 , , , cord-343256-n593kh7u 59 6 sera sera NNP cord-343256-n593kh7u 59 7 collected collect VBD cord-343256-n593kh7u 59 8 from from IN cord-343256-n593kh7u 59 9 spontaneous spontaneous JJ cord-343256-n593kh7u 59 10 cases case NNS cord-343256-n593kh7u 59 11 of of IN cord-343256-n593kh7u 59 12 SDA SDA NNP cord-343256-n593kh7u 59 13 were be VBD cord-343256-n593kh7u 59 14 used use VBN cord-343256-n593kh7u 59 15 to to TO cord-343256-n593kh7u 59 16 evaluate evaluate VB cord-343256-n593kh7u 59 17 the the DT cord-343256-n593kh7u 59 18 sensitivity sensitivity NN cord-343256-n593kh7u 59 19 of of IN cord-343256-n593kh7u 59 20 MHV MHV NNP cord-343256-n593kh7u 59 21 and and CC cord-343256-n593kh7u 59 22 SDAV SDAV NNP cord-343256-n593kh7u 59 23 - - HYPH cord-343256-n593kh7u 59 24 infected infect VBN cord-343256-n593kh7u 59 25 cells cell NNS cord-343256-n593kh7u 59 26 for for IN cord-343256-n593kh7u 59 27 antibody antibody NN cord-343256-n593kh7u 59 28 titration titration NN cord-343256-n593kh7u 59 29 . . . cord-343256-n593kh7u 60 1 The the DT cord-343256-n593kh7u 60 2 procedure procedure NN cord-343256-n593kh7u 60 3 , , , cord-343256-n593kh7u 60 4 using use VBG cord-343256-n593kh7u 60 5 horse horse NN cord-343256-n593kh7u 60 6 radish radish NN cord-343256-n593kh7u 60 7 peroxidase peroxidase NN cord-343256-n593kh7u 60 8 - - HYPH cord-343256-n593kh7u 60 9 conjugate conjugate JJ cord-343256-n593kh7u 60 10 anti anti JJ cord-343256-n593kh7u 60 11 - - JJ cord-343256-n593kh7u 60 12 rat rat JJ cord-343256-n593kh7u 60 13 IgG igg NN cord-343256-n593kh7u 60 14 , , , cord-343256-n593kh7u 60 15 has have VBZ cord-343256-n593kh7u 60 16 been be VBN cord-343256-n593kh7u 60 17 previously previously RB cord-343256-n593kh7u 60 18 described describe VBN cord-343256-n593kh7u 60 19 [ [ -LRB- cord-343256-n593kh7u 60 20 -7 -7 NNP cord-343256-n593kh7u 60 21 ] ] -RRB- cord-343256-n593kh7u 60 22 . . . cord-343256-n593kh7u 61 1 Typical typical JJ cord-343256-n593kh7u 61 2 changes change NNS cord-343256-n593kh7u 61 3 [ [ -LRB- cord-343256-n593kh7u 61 4 2 2 CD cord-343256-n593kh7u 61 5 , , , cord-343256-n593kh7u 61 6 8 8 CD cord-343256-n593kh7u 61 7 ] ] -RRB- cord-343256-n593kh7u 61 8 cells cell NNS cord-343256-n593kh7u 61 9 ( ( -LRB- cord-343256-n593kh7u 61 10 Fig Fig NNP cord-343256-n593kh7u 61 11 . . . cord-343256-n593kh7u 61 12 1 1 CD cord-343256-n593kh7u 61 13 a a LS cord-343256-n593kh7u 61 14 ) ) -RRB- cord-343256-n593kh7u 61 15 . . . cord-343256-n593kh7u 62 1 With with IN cord-343256-n593kh7u 62 2 L-2 L-2 NNP cord-343256-n593kh7u 62 3 cells cell NNS cord-343256-n593kh7u 62 4 inoculated inoculate VBN cord-343256-n593kh7u 62 5 with with IN cord-343256-n593kh7u 62 6 P p NN cord-343256-n593kh7u 62 7 R r NN cord-343256-n593kh7u 62 8 C c NN cord-343256-n593kh7u 62 9 and and CC cord-343256-n593kh7u 62 10 prepared prepare VBN cord-343256-n593kh7u 62 11 for for IN cord-343256-n593kh7u 62 12 the the DT cord-343256-n593kh7u 62 13 I I NNP cord-343256-n593kh7u 62 14 F F NNP cord-343256-n593kh7u 62 15 A A NNP cord-343256-n593kh7u 62 16 techniqe techniqe NN cord-343256-n593kh7u 62 17 , , , cord-343256-n593kh7u 62 18 cytoplasmic cytoplasmic JJ cord-343256-n593kh7u 62 19 fluorescence fluorescence NN cord-343256-n593kh7u 62 20 was be VBD cord-343256-n593kh7u 62 21 present present JJ cord-343256-n593kh7u 62 22 most most RBS cord-343256-n593kh7u 62 23 frequently frequently RB cord-343256-n593kh7u 62 24 in in IN cord-343256-n593kh7u 62 25 single single JJ cord-343256-n593kh7u 62 26 or or CC cord-343256-n593kh7u 62 27 binucleated binucleated JJ cord-343256-n593kh7u 62 28 cells cell NNS cord-343256-n593kh7u 62 29 ( ( -LRB- cord-343256-n593kh7u 62 30 Fig Fig NNP cord-343256-n593kh7u 62 31 . . . cord-343256-n593kh7u 62 32 1 1 CD cord-343256-n593kh7u 62 33 b b NN cord-343256-n593kh7u 62 34 ) ) -RRB- cord-343256-n593kh7u 62 35 . . . cord-343256-n593kh7u 63 1 Using use VBG cord-343256-n593kh7u 63 2 the the DT cord-343256-n593kh7u 63 3 I I NNP cord-343256-n593kh7u 63 4 F F NNP cord-343256-n593kh7u 63 5 A A NNP cord-343256-n593kh7u 63 6 procedure procedure NN cord-343256-n593kh7u 63 7 and and CC cord-343256-n593kh7u 63 8 MHV- MHV- NNP cord-343256-n593kh7u 63 9 , , , cord-343256-n593kh7u 63 10 SDAV- sdav- NN cord-343256-n593kh7u 63 11 , , , cord-343256-n593kh7u 63 12 or or CC cord-343256-n593kh7u 63 13 PRC PRC NNP cord-343256-n593kh7u 63 14 - - HYPH cord-343256-n593kh7u 63 15 infected infect VBN cord-343256-n593kh7u 63 16 cells cell NNS cord-343256-n593kh7u 63 17 as as IN cord-343256-n593kh7u 63 18 a a DT cord-343256-n593kh7u 63 19 source source NN cord-343256-n593kh7u 63 20 of of IN cord-343256-n593kh7u 63 21 antigen antigen NN cord-343256-n593kh7u 63 22 and and CC cord-343256-n593kh7u 63 23 sera sera NNP cord-343256-n593kh7u 63 24 from from IN cord-343256-n593kh7u 63 25 rats rat NNS cord-343256-n593kh7u 63 26 exposed expose VBN cord-343256-n593kh7u 63 27 to to IN cord-343256-n593kh7u 63 28 S S NNP cord-343256-n593kh7u 63 29 D D NNP cord-343256-n593kh7u 63 30 A A NNP cord-343256-n593kh7u 63 31 virus virus NN cord-343256-n593kh7u 63 32 , , , cord-343256-n593kh7u 63 33 antibody antibody NN cord-343256-n593kh7u 63 34 titers titer NNS cord-343256-n593kh7u 63 35 to to IN cord-343256-n593kh7u 63 36 all all DT cord-343256-n593kh7u 63 37 three three CD cord-343256-n593kh7u 63 38 antigens antigen NNS cord-343256-n593kh7u 63 39 were be VBD cord-343256-n593kh7u 63 40 similar similar JJ cord-343256-n593kh7u 63 41 ( ( -LRB- cord-343256-n593kh7u 63 42 Table table NN cord-343256-n593kh7u 63 43 1 1 CD cord-343256-n593kh7u 63 44 ) ) -RRB- cord-343256-n593kh7u 63 45 . . . cord-343256-n593kh7u 64 1 There there EX cord-343256-n593kh7u 64 2 was be VBD cord-343256-n593kh7u 64 3 no no DT cord-343256-n593kh7u 64 4 statistically statistically RB cord-343256-n593kh7u 64 5 - - HYPH cord-343256-n593kh7u 64 6 significant significant JJ cord-343256-n593kh7u 64 7 difference difference NN cord-343256-n593kh7u 64 8 in in IN cord-343256-n593kh7u 64 9 antibody antibody NN cord-343256-n593kh7u 64 10 titers titer NNS cord-343256-n593kh7u 64 11 to to IN cord-343256-n593kh7u 64 12 the the DT cord-343256-n593kh7u 64 13 three three CD cord-343256-n593kh7u 64 14 antigens antigen NNS cord-343256-n593kh7u 64 15 ( ( -LRB- cord-343256-n593kh7u 64 16 p p NN cord-343256-n593kh7u 64 17 < < XX cord-343256-n593kh7u 64 18 0.0001 0.0001 CD cord-343256-n593kh7u 64 19 ) ) -RRB- cord-343256-n593kh7u 64 20 . . . cord-343256-n593kh7u 65 1 Similarly similarly RB cord-343256-n593kh7u 65 2 , , , cord-343256-n593kh7u 65 3 in in IN cord-343256-n593kh7u 65 4 a a DT cord-343256-n593kh7u 65 5 comparison comparison NN cord-343256-n593kh7u 65 6 of of IN cord-343256-n593kh7u 65 7 antibody antibody NN cord-343256-n593kh7u 65 8 titers titer NNS cord-343256-n593kh7u 65 9 in in IN cord-343256-n593kh7u 65 10 rats rat NNS cord-343256-n593kh7u 65 11 inoculated inoculate VBN cord-343256-n593kh7u 65 12 with with IN cord-343256-n593kh7u 65 13 PRC PRC NNP cord-343256-n593kh7u 65 14 , , , cord-343256-n593kh7u 65 15 antibody antibody NN cord-343256-n593kh7u 65 16 titers titer NNS cord-343256-n593kh7u 65 17 were be VBD cord-343256-n593kh7u 65 18 similar similar JJ cord-343256-n593kh7u 65 19 ( ( -LRB- cord-343256-n593kh7u 65 20 Table table NN cord-343256-n593kh7u 65 21 2 2 CD cord-343256-n593kh7u 65 22 ) ) -RRB- cord-343256-n593kh7u 65 23 . . . cord-343256-n593kh7u 66 1 There there EX cord-343256-n593kh7u 66 2 was be VBD cord-343256-n593kh7u 66 3 no no DT cord-343256-n593kh7u 66 4 significant significant JJ cord-343256-n593kh7u 66 5 difference difference NN cord-343256-n593kh7u 66 6 in in IN cord-343256-n593kh7u 66 7 the the DT cord-343256-n593kh7u 66 8 m m NNP cord-343256-n593kh7u 66 9 e e NN cord-343256-n593kh7u 66 10 a a NN cord-343256-n593kh7u 66 11 n n NN cord-343256-n593kh7u 66 12 antibody antibody NN cord-343256-n593kh7u 66 13 titers titer NNS cord-343256-n593kh7u 66 14 to to IN cord-343256-n593kh7u 66 15 the the DT cord-343256-n593kh7u 66 16 homologous homologous JJ cord-343256-n593kh7u 66 17 virus virus NN cord-343256-n593kh7u 67 1 c c NNP cord-343256-n593kh7u 67 2 o o NN cord-343256-n593kh7u 67 3 m m NN cord-343256-n593kh7u 67 4 p p NN cord-343256-n593kh7u 67 5 a a NN cord-343256-n593kh7u 67 6 r r NN cord-343256-n593kh7u 67 7 e e NN cord-343256-n593kh7u 67 8 d d NN cord-343256-n593kh7u 67 9 to to IN cord-343256-n593kh7u 67 10 the the DT cord-343256-n593kh7u 67 11 heterologous heterologous JJ cord-343256-n593kh7u 67 12 viral viral JJ cord-343256-n593kh7u 67 13 antigens antigen NNS cord-343256-n593kh7u 67 14 ( ( -LRB- cord-343256-n593kh7u 67 15 p p NN cord-343256-n593kh7u 67 16 < < XX cord-343256-n593kh7u 67 17 0.0001 0.0001 CD cord-343256-n593kh7u 67 18 ) ) -RRB- cord-343256-n593kh7u 67 19 . . . cord-343256-n593kh7u 68 1 Using use VBG cord-343256-n593kh7u 68 2 MHV MHV NNP cord-343256-n593kh7u 68 3 - - HYPH cord-343256-n593kh7u 68 4 infected infect VBN cord-343256-n593kh7u 68 5 N n NN cord-343256-n593kh7u 68 6 C c NN cord-343256-n593kh7u 68 7 T t NN cord-343256-n593kh7u 68 8 C c NN cord-343256-n593kh7u 68 9 cells cell NNS cord-343256-n593kh7u 68 10 and and CC cord-343256-n593kh7u 68 11 SDAV SDAV NNP cord-343256-n593kh7u 68 12 - - HYPH cord-343256-n593kh7u 68 13 infected infect VBN cord-343256-n593kh7u 68 14 L-2 L-2 NNP cord-343256-n593kh7u 68 15 cells cell NNS cord-343256-n593kh7u 68 16 as as IN cord-343256-n593kh7u 68 17 the the DT cord-343256-n593kh7u 68 18 source source NN cord-343256-n593kh7u 68 19 of of IN cord-343256-n593kh7u 68 20 antigen antigen NN cord-343256-n593kh7u 68 21 , , , cord-343256-n593kh7u 68 22 serum serum NN cord-343256-n593kh7u 68 23 antibody antibody NN cord-343256-n593kh7u 68 24 titers titer NNS cord-343256-n593kh7u 68 25 of of IN cord-343256-n593kh7u 68 26 SDAV SDAV NNP cord-343256-n593kh7u 68 27 - - HYPH cord-343256-n593kh7u 68 28 infected infect VBN cord-343256-n593kh7u 68 29 rats rat NNS cord-343256-n593kh7u 68 30 were be VBD cord-343256-n593kh7u 68 31 usually usually RB cord-343256-n593kh7u 68 32 substantially substantially RB cord-343256-n593kh7u 68 33 higher high JJR cord-343256-n593kh7u 68 34 with with IN cord-343256-n593kh7u 68 35 homologous homologous JJ cord-343256-n593kh7u 68 36 antigen antigen NN cord-343256-n593kh7u 68 37 than than IN cord-343256-n593kh7u 68 38 with with IN cord-343256-n593kh7u 68 39 the the DT cord-343256-n593kh7u 68 40 MHV MHV NNP cord-343256-n593kh7u 68 41 - - HYPH cord-343256-n593kh7u 68 42 infected infect VBN cord-343256-n593kh7u 68 43 cells cell NNS cord-343256-n593kh7u 68 44 ( ( -LRB- cord-343256-n593kh7u 68 45 Table Table NNP cord-343256-n593kh7u 68 46 3 3 CD cord-343256-n593kh7u 68 47 ) ) -RRB- cord-343256-n593kh7u 68 48 . . . cord-343256-n593kh7u 69 1 In in IN cord-343256-n593kh7u 69 2 a a DT cord-343256-n593kh7u 69 3 comparison comparison NN cord-343256-n593kh7u 69 4 of of IN cord-343256-n593kh7u 69 5 the the DT cord-343256-n593kh7u 69 6 m m NNP cord-343256-n593kh7u 69 7 e e NNP cord-343256-n593kh7u 69 8 a a NN cord-343256-n593kh7u 69 9 n n CD cord-343256-n593kh7u 69 10 antibody antibody NN cord-343256-n593kh7u 69 11 titers titer NNS cord-343256-n593kh7u 69 12 , , , cord-343256-n593kh7u 69 13 the the DT cord-343256-n593kh7u 69 14 antibody antibody NN cord-343256-n593kh7u 69 15 titers titer NNS cord-343256-n593kh7u 69 16 to to IN cord-343256-n593kh7u 69 17 480 480 CD cord-343256-n593kh7u 69 18 _SP cord-343256-n593kh7u 69 19 320 320 CD cord-343256-n593kh7u 69 20 _SP cord-343256-n593kh7u 69 21 2 2 CD cord-343256-n593kh7u 69 22 _SP cord-343256-n593kh7u 69 23 2560 2560 CD cord-343256-n593kh7u 69 24 _SP cord-343256-n593kh7u 70 1 2560 2560 CD cord-343256-n593kh7u 70 2 _SP cord-343256-n593kh7u 71 1 640 640 CD cord-343256-n593kh7u 71 2 _SP cord-343256-n593kh7u 72 1 3 3 CD cord-343256-n593kh7u 72 2 _SP cord-343256-n593kh7u 73 1 640 640 CD cord-343256-n593kh7u 73 2 _SP cord-343256-n593kh7u 73 3 640 640 CD cord-343256-n593kh7u 73 4 _SP cord-343256-n593kh7u 74 1 1280 1280 CD cord-343256-n593kh7u 74 2 _SP cord-343256-n593kh7u 75 1 4 4 CD cord-343256-n593kh7u 75 2 _SP cord-343256-n593kh7u 76 1 80 80 CD cord-343256-n593kh7u 76 2 _SP cord-343256-n593kh7u 76 3 40 40 CD cord-343256-n593kh7u 76 4 _SP cord-343256-n593kh7u 76 5 40 40 CD cord-343256-n593kh7u 76 6 _SP cord-343256-n593kh7u 77 1 5 5 CD cord-343256-n593kh7u 77 2 _SP cord-343256-n593kh7u 78 1 40 40 CD cord-343256-n593kh7u 78 2 _SP cord-343256-n593kh7u 78 3 40 40 CD cord-343256-n593kh7u 78 4 _SP cord-343256-n593kh7u 78 5 40 40 CD cord-343256-n593kh7u 78 6 _SP cord-343256-n593kh7u 79 1 6 6 CD cord-343256-n593kh7u 79 2 _SP cord-343256-n593kh7u 80 1 80 80 CD cord-343256-n593kh7u 80 2 _SP cord-343256-n593kh7u 81 1 40 40 CD cord-343256-n593kh7u 81 2 _SP cord-343256-n593kh7u 81 3 80 80 CD cord-343256-n593kh7u 81 4 _SP cord-343256-n593kh7u 81 5 7 7 CD cord-343256-n593kh7u 81 6 _SP cord-343256-n593kh7u 81 7 80 80 CD cord-343256-n593kh7u 81 8 _SP cord-343256-n593kh7u 81 9 80 80 CD cord-343256-n593kh7u 81 10 _SP cord-343256-n593kh7u 81 11 80 80 CD cord-343256-n593kh7u 81 12 _SP cord-343256-n593kh7u 82 1 8 8 CD cord-343256-n593kh7u 82 2 _SP cord-343256-n593kh7u 83 1 160 160 CD cord-343256-n593kh7u 83 2 _SP cord-343256-n593kh7u 83 3 160 160 CD cord-343256-n593kh7u 83 4 _SP cord-343256-n593kh7u 84 1 160 160 CD cord-343256-n593kh7u 84 2 _SP cord-343256-n593kh7u 85 1 9 9 CD cord-343256-n593kh7u 85 2 _SP cord-343256-n593kh7u 86 1 80 80 CD cord-343256-n593kh7u 86 2 _SP cord-343256-n593kh7u 87 1 80 80 CD cord-343256-n593kh7u 87 2 _SP cord-343256-n593kh7u 88 1 80 80 CD cord-343256-n593kh7u 88 2 _SP cord-343256-n593kh7u 89 1 10 10 CD cord-343256-n593kh7u 89 2 _SP cord-343256-n593kh7u 90 1 160 160 CD cord-343256-n593kh7u 90 2 _SP cord-343256-n593kh7u 90 3 160 160 CD cord-343256-n593kh7u 90 4 _SP cord-343256-n593kh7u 91 1 160 160 CD cord-343256-n593kh7u 91 2 _SP cord-343256-n593kh7u 91 3 11 11 CD cord-343256-n593kh7u 91 4 160 160 CD cord-343256-n593kh7u 91 5 160 160 CD cord-343256-n593kh7u 91 6 160 160 CD cord-343256-n593kh7u 92 1 a a DT cord-343256-n593kh7u 92 2 Sialodacryoadenitis Sialodacryoadenitis NNP cord-343256-n593kh7u 92 3 virus virus NN cord-343256-n593kh7u 92 4 b b IN cord-343256-n593kh7u 92 5 Parker Parker NNP cord-343256-n593kh7u 92 6 's 's POS cord-343256-n593kh7u 92 7 rat rat NN cord-343256-n593kh7u 92 8 coronavirus coronavirus NN cord-343256-n593kh7u 92 9 c c NN cord-343256-n593kh7u 92 10 Mouse Mouse NNP cord-343256-n593kh7u 92 11 hepatitis hepatitis NN cord-343256-n593kh7u 92 12 virus virus NN cord-343256-n593kh7u 93 1 * * NFP cord-343256-n593kh7u 94 1 No no DT cord-343256-n593kh7u 94 2 significant significant JJ cord-343256-n593kh7u 94 3 difference difference NN cord-343256-n593kh7u 94 4 in in IN cord-343256-n593kh7u 94 5 the the DT cord-343256-n593kh7u 94 6 means mean NNS cord-343256-n593kh7u 94 7 of of IN cord-343256-n593kh7u 94 8 the the DT cord-343256-n593kh7u 94 9 antibody antibody NN cord-343256-n593kh7u 94 10 titers titer NNS cord-343256-n593kh7u 94 11 to to IN cord-343256-n593kh7u 94 12 the the DT cord-343256-n593kh7u 94 13 three three CD cord-343256-n593kh7u 94 14 viral viral JJ cord-343256-n593kh7u 94 15 antigens antigen NNS cord-343256-n593kh7u 94 16 ( ( -LRB- cord-343256-n593kh7u 94 17 p p NN cord-343256-n593kh7u 94 18 < < XX cord-343256-n593kh7u 94 19 0.0001 0.0001 CD cord-343256-n593kh7u 94 20 ) ) -RRB- cord-343256-n593kh7u 94 21 Table table NN cord-343256-n593kh7u 94 22 2 2 CD cord-343256-n593kh7u 94 23 . . . cord-343256-n593kh7u 95 1 Comparison comparison NN cord-343256-n593kh7u 95 2 of of IN cord-343256-n593kh7u 95 3 antibody antibody NN cord-343256-n593kh7u 95 4 titers titer NNS cord-343256-n593kh7u 95 5 in in IN cord-343256-n593kh7u 95 6 rats rat NNS cord-343256-n593kh7u 95 7 exposed expose VBN cord-343256-n593kh7u 95 8 to to IN cord-343256-n593kh7u 95 9 Parker Parker NNP cord-343256-n593kh7u 95 10 's 's POS cord-343256-n593kh7u 95 11 rat rat NN cord-343256-n593kh7u 95 12 coronavirus coronavirus NN cord-343256-n593kh7u 95 13 using use VBG cord-343256-n593kh7u 95 14 SDAV SDAV NNP cord-343256-n593kh7u 95 15 , , , cord-343256-n593kh7u 95 16 PRC PRC NNP cord-343256-n593kh7u 95 17 , , , cord-343256-n593kh7u 95 18 and and CC cord-343256-n593kh7u 95 19 MHV mhv NN cord-343256-n593kh7u 95 20 - - HYPH cord-343256-n593kh7u 95 21 infected infect VBN cord-343256-n593kh7u 95 22 cells cell NNS cord-343256-n593kh7u 95 23 0 0 CD cord-343256-n593kh7u 95 24 _SP cord-343256-n593kh7u 95 25 0 0 CD cord-343256-n593kh7u 95 26 _SP cord-343256-n593kh7u 95 27 7 7 CD cord-343256-n593kh7u 95 28 _SP cord-343256-n593kh7u 96 1 640 640 CD cord-343256-n593kh7u 96 2 _SP cord-343256-n593kh7u 97 1 320 320 CD cord-343256-n593kh7u 97 2 _SP cord-343256-n593kh7u 98 1 640 640 CD cord-343256-n593kh7u 98 2 _SP cord-343256-n593kh7u 99 1 8 8 CD cord-343256-n593kh7u 99 2 _SP cord-343256-n593kh7u 100 1 640 640 CD cord-343256-n593kh7u 100 2 _SP cord-343256-n593kh7u 101 1 320 320 CD cord-343256-n593kh7u 101 2 _SP cord-343256-n593kh7u 102 1 320 320 CD cord-343256-n593kh7u 102 2 _SP cord-343256-n593kh7u 102 3 13 13 CD cord-343256-n593kh7u 102 4 _SP cord-343256-n593kh7u 103 1 40 40 CD cord-343256-n593kh7u 103 2 _SP cord-343256-n593kh7u 103 3 40 40 CD cord-343256-n593kh7u 103 4 _SP cord-343256-n593kh7u 104 1 40 40 CD cord-343256-n593kh7u 104 2 _SP cord-343256-n593kh7u 104 3 15 15 CD cord-343256-n593kh7u 104 4 _SP cord-343256-n593kh7u 104 5 320 320 CD cord-343256-n593kh7u 104 6 _SP cord-343256-n593kh7u 104 7 320 320 CD cord-343256-n593kh7u 104 8 _SP cord-343256-n593kh7u 105 1 320 320 CD cord-343256-n593kh7u 105 2 _SP cord-343256-n593kh7u 105 3 16 16 CD cord-343256-n593kh7u 105 4 _SP cord-343256-n593kh7u 105 5 320 320 CD cord-343256-n593kh7u 105 6 _SP cord-343256-n593kh7u 105 7 320 320 CD cord-343256-n593kh7u 105 8 _SP cord-343256-n593kh7u 105 9 640 640 CD cord-343256-n593kh7u 105 10 _SP cord-343256-n593kh7u 105 11 20 20 CD cord-343256-n593kh7u 105 12 _SP cord-343256-n593kh7u 106 1 640 640 CD cord-343256-n593kh7u 106 2 _SP cord-343256-n593kh7u 107 1 640 640 CD cord-343256-n593kh7u 107 2 _SP cord-343256-n593kh7u 108 1 320 320 CD cord-343256-n593kh7u 108 2 _SP cord-343256-n593kh7u 109 1 9 9 CD cord-343256-n593kh7u 109 2 _SP cord-343256-n593kh7u 110 1 80 80 CD cord-343256-n593kh7u 110 2 _SP cord-343256-n593kh7u 111 1 80 80 CD cord-343256-n593kh7u 111 2 _SP cord-343256-n593kh7u 112 1 40 40 CD cord-343256-n593kh7u 112 2 _SP cord-343256-n593kh7u 112 3 10 10 CD cord-343256-n593kh7u 112 4 _SP cord-343256-n593kh7u 113 1 80 80 CD cord-343256-n593kh7u 113 2 _SP cord-343256-n593kh7u 114 1 80 80 CD cord-343256-n593kh7u 114 2 _SP cord-343256-n593kh7u 115 1 80 80 CD cord-343256-n593kh7u 115 2 _SP cord-343256-n593kh7u 116 1 11 11 CD cord-343256-n593kh7u 116 2 _SP cord-343256-n593kh7u 117 1 80 80 CD cord-343256-n593kh7u 117 2 _SP cord-343256-n593kh7u 118 1 80 80 CD cord-343256-n593kh7u 118 2 _SP cord-343256-n593kh7u 119 1 80 80 CD cord-343256-n593kh7u 119 2 _SP cord-343256-n593kh7u 120 1 12 12 CD cord-343256-n593kh7u 120 2 _SP cord-343256-n593kh7u 121 1 40 40 CD cord-343256-n593kh7u 121 2 _SP cord-343256-n593kh7u 121 3 80 80 CD cord-343256-n593kh7u 121 4 _SP cord-343256-n593kh7u 122 1 40 40 CD cord-343256-n593kh7u 123 1 * * NFP cord-343256-n593kh7u 124 1 No no DT cord-343256-n593kh7u 124 2 significant significant JJ cord-343256-n593kh7u 124 3 difference difference NN cord-343256-n593kh7u 124 4 in in IN cord-343256-n593kh7u 124 5 the the DT cord-343256-n593kh7u 124 6 means mean NNS cord-343256-n593kh7u 124 7 of of IN cord-343256-n593kh7u 124 8 the the DT cord-343256-n593kh7u 124 9 antibody antibody NN cord-343256-n593kh7u 124 10 titers titer NNS cord-343256-n593kh7u 124 11 to to IN cord-343256-n593kh7u 124 12 the the DT cord-343256-n593kh7u 124 13 three three CD cord-343256-n593kh7u 124 14 viral viral JJ cord-343256-n593kh7u 124 15 antigens antigen NNS cord-343256-n593kh7u 124 16 ( ( -LRB- cord-343256-n593kh7u 124 17 p p NN cord-343256-n593kh7u 124 18 < < XX cord-343256-n593kh7u 124 19 0.0001 0.0001 CD cord-343256-n593kh7u 124 20 ) ) -RRB- cord-343256-n593kh7u 124 21 the the DT cord-343256-n593kh7u 124 22 homologous homologous JJ cord-343256-n593kh7u 124 23 viral viral JJ cord-343256-n593kh7u 124 24 antigen antigen NN cord-343256-n593kh7u 124 25 were be VBD cord-343256-n593kh7u 124 26 significantly significantly RB cord-343256-n593kh7u 124 27 higher high JJR cord-343256-n593kh7u 124 28 than than IN cord-343256-n593kh7u 124 29 those those DT cord-343256-n593kh7u 124 30 detected detect VBN cord-343256-n593kh7u 124 31 using use VBG cord-343256-n593kh7u 124 32 the the DT cord-343256-n593kh7u 124 33 heterologous heterologous JJ cord-343256-n593kh7u 124 34 virus virus NN cord-343256-n593kh7u 124 35 ( ( -LRB- cord-343256-n593kh7u 124 36 p p NN cord-343256-n593kh7u 124 37 < < XX cord-343256-n593kh7u 124 38 0.0046 0.0046 XX cord-343256-n593kh7u 124 39 ) ) -RRB- cord-343256-n593kh7u 124 40 . . . cord-343256-n593kh7u 125 1 The the DT cord-343256-n593kh7u 125 2 ability ability NN cord-343256-n593kh7u 125 3 to to TO cord-343256-n593kh7u 125 4 replicate replicate VB cord-343256-n593kh7u 125 5 relatively relatively RB cord-343256-n593kh7u 125 6 high high JJ cord-343256-n593kh7u 125 7 titers titer NNS cord-343256-n593kh7u 125 8 of of IN cord-343256-n593kh7u 125 9 rat rat NN cord-343256-n593kh7u 125 10 coronaviruses coronaviruse NNS cord-343256-n593kh7u 125 11 in in IN cord-343256-n593kh7u 125 12 a a DT cord-343256-n593kh7u 125 13 continuous continuous JJ cord-343256-n593kh7u 125 14 cell cell NN cord-343256-n593kh7u 125 15 line line NN cord-343256-n593kh7u 125 16 has have VBZ cord-343256-n593kh7u 125 17 provided provide VBN cord-343256-n593kh7u 125 18 the the DT cord-343256-n593kh7u 125 19 opportunity opportunity NN cord-343256-n593kh7u 125 20 to to TO cord-343256-n593kh7u 125 21 evaluate evaluate VB cord-343256-n593kh7u 125 22 the the DT cord-343256-n593kh7u 125 23 sensitivity sensitivity NN cord-343256-n593kh7u 125 24 and and CC cord-343256-n593kh7u 125 25 specificity specificity NN cord-343256-n593kh7u 125 26 of of IN cord-343256-n593kh7u 125 27 rat rat NN cord-343256-n593kh7u 125 28 coronaviral coronaviral RB cord-343256-n593kh7u 125 29 - - HYPH cord-343256-n593kh7u 125 30 infected infect VBN cord-343256-n593kh7u 125 31 cells cell NNS cord-343256-n593kh7u 125 32 compared compare VBN cord-343256-n593kh7u 125 33 to to IN cord-343256-n593kh7u 125 34 MHV MHV NNP cord-343256-n593kh7u 125 35 - - HYPH cord-343256-n593kh7u 125 36 infected infect VBN cord-343256-n593kh7u 125 37 cells cell NNS cord-343256-n593kh7u 125 38 as as IN cord-343256-n593kh7u 125 39 a a DT cord-343256-n593kh7u 125 40 source source NN cord-343256-n593kh7u 125 41 of of IN cord-343256-n593kh7u 125 42 antigen antigen NN cord-343256-n593kh7u 125 43 for for IN cord-343256-n593kh7u 125 44 the the DT cord-343256-n593kh7u 125 45 detection detection NN cord-343256-n593kh7u 125 46 and and CC cord-343256-n593kh7u 125 47 titration titration NN cord-343256-n593kh7u 125 48 of of IN cord-343256-n593kh7u 125 49 antibody antibody NN cord-343256-n593kh7u 125 50 to to IN cord-343256-n593kh7u 125 51 SDAV SDAV NNP cord-343256-n593kh7u 125 52 and and CC cord-343256-n593kh7u 125 53 PRC PRC NNP cord-343256-n593kh7u 125 54 . . . cord-343256-n593kh7u 126 1 The the DT cord-343256-n593kh7u 126 2 antigenic antigenic JJ cord-343256-n593kh7u 126 3 similarities similarity NNS cord-343256-n593kh7u 126 4 among among IN cord-343256-n593kh7u 126 5 the the DT cord-343256-n593kh7u 126 6 rodent rodent NN cord-343256-n593kh7u 126 7 coronaviruses coronaviruse NNS cord-343256-n593kh7u 126 8 have have VBP cord-343256-n593kh7u 126 9 been be VBN cord-343256-n593kh7u 126 10 emphasized emphasize VBN cord-343256-n593kh7u 126 11 in in IN cord-343256-n593kh7u 126 12 previous previous JJ cord-343256-n593kh7u 126 13 studies study NNS cord-343256-n593kh7u 126 14 [ [ -LRB- cord-343256-n593kh7u 126 15 1 1 CD cord-343256-n593kh7u 126 16 , , , cord-343256-n593kh7u 126 17 3 3 CD cord-343256-n593kh7u 126 18 ] ] -RRB- cord-343256-n593kh7u 126 19 , , , cord-343256-n593kh7u 126 20 thus thus RB cord-343256-n593kh7u 126 21 allowing allow VBG cord-343256-n593kh7u 126 22 the the DT cord-343256-n593kh7u 126 23 use use NN cord-343256-n593kh7u 126 24 of of IN cord-343256-n593kh7u 126 25 MHV mhv NN cord-343256-n593kh7u 126 26 - - HYPH cord-343256-n593kh7u 126 27 infected infect VBN cord-343256-n593kh7u 126 28 cells cell NNS cord-343256-n593kh7u 126 29 as as IN cord-343256-n593kh7u 126 30 a a DT cord-343256-n593kh7u 126 31 source source NN cord-343256-n593kh7u 126 32 of of IN cord-343256-n593kh7u 126 33 antigen antigen NN cord-343256-n593kh7u 126 34 for for IN cord-343256-n593kh7u 126 35 the the DT cord-343256-n593kh7u 126 36 detection detection NN cord-343256-n593kh7u 126 37 of of IN cord-343256-n593kh7u 126 38 antibodies antibody NNS cord-343256-n593kh7u 126 39 to to IN cord-343256-n593kh7u 126 40 either either CC cord-343256-n593kh7u 126 41 mouse mouse NN cord-343256-n593kh7u 126 42 or or CC cord-343256-n593kh7u 126 43 rat rat NN cord-343256-n593kh7u 126 44 coronaviruses coronaviruse NNS cord-343256-n593kh7u 126 45 [ [ -LRB- cord-343256-n593kh7u 126 46 6 6 CD cord-343256-n593kh7u 126 47 ] ] -RRB- cord-343256-n593kh7u 126 48 . . . cord-343256-n593kh7u 127 1 Based base VBN cord-343256-n593kh7u 127 2 on on IN cord-343256-n593kh7u 127 3 our -PRON- PRP$ cord-343256-n593kh7u 127 4 studies study NNS cord-343256-n593kh7u 127 5 , , , cord-343256-n593kh7u 127 6 the the DT cord-343256-n593kh7u 127 7 use use NN cord-343256-n593kh7u 127 8 of of IN cord-343256-n593kh7u 127 9 rat rat NN cord-343256-n593kh7u 127 10 coronavirus coronavirus NN cord-343256-n593kh7u 127 11 - - HYPH cord-343256-n593kh7u 127 12 infected infect VBN cord-343256-n593kh7u 127 13 cells cell NNS cord-343256-n593kh7u 127 14 as as IN cord-343256-n593kh7u 127 15 a a DT cord-343256-n593kh7u 127 16 source source NN cord-343256-n593kh7u 127 17 of of IN cord-343256-n593kh7u 127 18 antigen antigen NN cord-343256-n593kh7u 127 19 offers offer VBZ cord-343256-n593kh7u 127 20 no no DT cord-343256-n593kh7u 127 21 significant significant JJ cord-343256-n593kh7u 127 22 advantage advantage NN cord-343256-n593kh7u 127 23 over over IN cord-343256-n593kh7u 127 24 MHV mhv NN cord-343256-n593kh7u 127 25 - - HYPH cord-343256-n593kh7u 127 26 infected infect VBN cord-343256-n593kh7u 127 27 cells cell NNS cord-343256-n593kh7u 127 28 . . . cord-343256-n593kh7u 128 1 Furthermore furthermore RB cord-343256-n593kh7u 128 2 , , , cord-343256-n593kh7u 128 3 we -PRON- PRP cord-343256-n593kh7u 128 4 were be VBD cord-343256-n593kh7u 128 5 unable unable JJ cord-343256-n593kh7u 128 6 to to TO cord-343256-n593kh7u 128 7 demonstrate demonstrate VB cord-343256-n593kh7u 128 8 any any DT cord-343256-n593kh7u 128 9 appreciable appreciable JJ cord-343256-n593kh7u 128 10 advantage advantage NN cord-343256-n593kh7u 128 11 in in IN cord-343256-n593kh7u 128 12 using use VBG cord-343256-n593kh7u 128 13 either either CC cord-343256-n593kh7u 128 14 SDAV SDAV NNP cord-343256-n593kh7u 128 15 or or CC cord-343256-n593kh7u 128 16 PRC PRC NNP cord-343256-n593kh7u 128 17 as as IN cord-343256-n593kh7u 128 18 a a DT cord-343256-n593kh7u 128 19 source source NN cord-343256-n593kh7u 128 20 of of IN cord-343256-n593kh7u 128 21 viral viral JJ cord-343256-n593kh7u 128 22 antigen antigen NN cord-343256-n593kh7u 128 23 to to TO cord-343256-n593kh7u 128 24 detect detect VB cord-343256-n593kh7u 128 25 antibody antibody NN cord-343256-n593kh7u 128 26 to to IN cord-343256-n593kh7u 128 27 the the DT cord-343256-n593kh7u 128 28 homologous homologous JJ cord-343256-n593kh7u 128 29 virus virus NN cord-343256-n593kh7u 128 30 , , , cord-343256-n593kh7u 128 31 either either CC cord-343256-n593kh7u 128 32 in in IN cord-343256-n593kh7u 128 33 sensitivity sensitivity NN cord-343256-n593kh7u 128 34 or or CC cord-343256-n593kh7u 128 35 specificity specificity NN cord-343256-n593kh7u 128 36 . . . cord-343256-n593kh7u 129 1 Based base VBN cord-343256-n593kh7u 129 2 on on IN cord-343256-n593kh7u 129 3 these these DT cord-343256-n593kh7u 129 4 data datum NNS cord-343256-n593kh7u 129 5 , , , cord-343256-n593kh7u 129 6 there there EX cord-343256-n593kh7u 129 7 is be VBZ cord-343256-n593kh7u 129 8 no no DT cord-343256-n593kh7u 129 9 evidence evidence NN cord-343256-n593kh7u 129 10 that that IN cord-343256-n593kh7u 129 11 IFA IFA NNP cord-343256-n593kh7u 129 12 staining staining NN cord-343256-n593kh7u 129 13 of of IN cord-343256-n593kh7u 129 14 L-2 L-2 NNP cord-343256-n593kh7u 129 15 cells cell NNS cord-343256-n593kh7u 129 16 infected infect VBN cord-343256-n593kh7u 129 17 with with IN cord-343256-n593kh7u 129 18 either either CC cord-343256-n593kh7u 129 19 SDAV SDAV NNP cord-343256-n593kh7u 129 20 or or CC cord-343256-n593kh7u 129 21 PRC PRC NNP cord-343256-n593kh7u 129 22 will will MD cord-343256-n593kh7u 129 23 serve serve VB cord-343256-n593kh7u 129 24 to to TO cord-343256-n593kh7u 129 25 differentiate differentiate VB cord-343256-n593kh7u 129 26 antibody antibody NN cord-343256-n593kh7u 129 27 to to IN cord-343256-n593kh7u 129 28 the the DT cord-343256-n593kh7u 129 29 homologous homologous JJ cord-343256-n593kh7u 129 30 or or CC cord-343256-n593kh7u 129 31 heterologous heterologous JJ cord-343256-n593kh7u 129 32 virus virus NN cord-343256-n593kh7u 129 33 . . . cord-343256-n593kh7u 130 1 However however RB cord-343256-n593kh7u 130 2 , , , cord-343256-n593kh7u 130 3 using use VBG cord-343256-n593kh7u 130 4 M M NNP cord-343256-n593kh7u 130 5 H H NNP cord-343256-n593kh7u 130 6 V V NNP cord-343256-n593kh7u 130 7 or or CC cord-343256-n593kh7u 130 8 SDAV SDAV NNP cord-343256-n593kh7u 130 9 - - HYPH cord-343256-n593kh7u 130 10 infected infect VBN cord-343256-n593kh7u 130 11 cells cell NNS cord-343256-n593kh7u 130 12 as as IN cord-343256-n593kh7u 130 13 a a DT cord-343256-n593kh7u 130 14 source source NN cord-343256-n593kh7u 130 15 of of IN cord-343256-n593kh7u 130 16 antigen antigen NN cord-343256-n593kh7u 130 17 for for IN cord-343256-n593kh7u 130 18 the the DT cord-343256-n593kh7u 130 19 enzyme enzyme NN cord-343256-n593kh7u 130 20 immunoassay immunoassay NN cord-343256-n593kh7u 130 21 procedure procedure NN cord-343256-n593kh7u 130 22 , , , cord-343256-n593kh7u 130 23 there there EX cord-343256-n593kh7u 130 24 was be VBD cord-343256-n593kh7u 130 25 a a DT cord-343256-n593kh7u 130 26 significant significant JJ cord-343256-n593kh7u 130 27 difference difference NN cord-343256-n593kh7u 130 28 in in IN cord-343256-n593kh7u 130 29 the the DT cord-343256-n593kh7u 130 30 anti anti JJ cord-343256-n593kh7u 130 31 - - JJ cord-343256-n593kh7u 130 32 SDAV sdav JJ cord-343256-n593kh7u 130 33 antibody antibody NN cord-343256-n593kh7u 130 34 titers titer NNS cord-343256-n593kh7u 130 35 using use VBG cord-343256-n593kh7u 130 36 the the DT cord-343256-n593kh7u 130 37 homologous homologous JJ cord-343256-n593kh7u 130 38 virus virus NN cord-343256-n593kh7u 130 39 ( ( -LRB- cord-343256-n593kh7u 130 40 p p NN cord-343256-n593kh7u 130 41 < < XX cord-343256-n593kh7u 130 42 0.0046 0.0046 XX cord-343256-n593kh7u 130 43 ) ) -RRB- cord-343256-n593kh7u 130 44 . . . cord-343256-n593kh7u 131 1 SDA SDA NNP cord-343256-n593kh7u 131 2 antibody antibody NN cord-343256-n593kh7u 131 3 titers titer NNS cord-343256-n593kh7u 131 4 were be VBD cord-343256-n593kh7u 131 5 usually usually RB cord-343256-n593kh7u 131 6 considerably considerably RB cord-343256-n593kh7u 131 7 higher higher RBR cord-343256-n593kh7u 131 8 using use VBG cord-343256-n593kh7u 131 9 the the DT cord-343256-n593kh7u 131 10 enzyme enzyme NN cord-343256-n593kh7u 131 11 immunoassay immunoassay NN cord-343256-n593kh7u 131 12 procedure procedure NN cord-343256-n593kh7u 131 13 with with IN cord-343256-n593kh7u 131 14 homologous homologous JJ cord-343256-n593kh7u 131 15 antigen antigen NN cord-343256-n593kh7u 131 16 than than IN cord-343256-n593kh7u 131 17 with with IN cord-343256-n593kh7u 131 18 MHV mhv NN cord-343256-n593kh7u 131 19 - - HYPH cord-343256-n593kh7u 131 20 infected infect VBN cord-343256-n593kh7u 131 21 cells cell NNS cord-343256-n593kh7u 131 22 . . . cord-343256-n593kh7u 132 1 Characterization characterization NN cord-343256-n593kh7u 132 2 of of IN cord-343256-n593kh7u 132 3 the the DT cord-343256-n593kh7u 132 4 virus virus NN cord-343256-n593kh7u 132 5 of of IN cord-343256-n593kh7u 132 6 sialodaeryoadenitis sialodaeryoadenitis NNP cord-343256-n593kh7u 132 7 of of IN cord-343256-n593kh7u 132 8 rats rat NNS cord-343256-n593kh7u 132 9 : : : cord-343256-n593kh7u 132 10 a a DT cord-343256-n593kh7u 132 11 member member NN cord-343256-n593kh7u 132 12 of of IN cord-343256-n593kh7u 132 13 the the DT cord-343256-n593kh7u 132 14 coronavirus coronavirus NN cord-343256-n593kh7u 132 15 group group NN cord-343256-n593kh7u 132 16 The the DT cord-343256-n593kh7u 132 17 laboratory laboratory NN cord-343256-n593kh7u 132 18 rat rat NN cord-343256-n593kh7u 132 19 . . . cord-343256-n593kh7u 133 1 Biology biology NN cord-343256-n593kh7u 133 2 and and CC cord-343256-n593kh7u 133 3 diseases disease NNS cord-343256-n593kh7u 133 4 Rat Rat NNP cord-343256-n593kh7u 133 5 coronavirus coronavirus NN cord-343256-n593kh7u 133 6 ( ( -LRB- cord-343256-n593kh7u 133 7 RCV RCV NNP cord-343256-n593kh7u 133 8 ) ) -RRB- cord-343256-n593kh7u 133 9 : : : cord-343256-n593kh7u 134 1 a a DT cord-343256-n593kh7u 134 2 prevalent prevalent JJ cord-343256-n593kh7u 134 3 , , , cord-343256-n593kh7u 134 4 naturallyoccurring naturallyoccurre VBG cord-343256-n593kh7u 134 5 pneumotropic pneumotropic NNP cord-343256-n593kh7u 134 6 virus virus NN cord-343256-n593kh7u 134 7 of of IN cord-343256-n593kh7u 134 8 rats rat NNS cord-343256-n593kh7u 134 9 Replication Replication NNP cord-343256-n593kh7u 134 10 of of IN cord-343256-n593kh7u 134 11 sialodacryoadenitis sialodacryoadenitis NNP cord-343256-n593kh7u 134 12 virus virus NN cord-343256-n593kh7u 134 13 in in IN cord-343256-n593kh7u 134 14 mouse mouse NN cord-343256-n593kh7u 134 15 L-2 L-2 NNP cord-343256-n593kh7u 134 16 cells cell NNS cord-343256-n593kh7u 134 17 Characteristics characteristic NNS cord-343256-n593kh7u 134 18 of of IN cord-343256-n593kh7u 134 19 Parker Parker NNP cord-343256-n593kh7u 134 20 's 's POS cord-343256-n593kh7u 134 21 rat rat NN cord-343256-n593kh7u 134 22 coronavirus coronavirus NN cord-343256-n593kh7u 134 23 ( ( -LRB- cord-343256-n593kh7u 134 24 PRC PRC NNP cord-343256-n593kh7u 134 25 ) ) -RRB- cord-343256-n593kh7u 134 26 replicated replicate VBD cord-343256-n593kh7u 134 27 in in IN cord-343256-n593kh7u 134 28 L-2 L-2 NNP cord-343256-n593kh7u 134 29 cells cell NNS cord-343256-n593kh7u 135 1 An an DT cord-343256-n593kh7u 135 2 immunofluorescence immunofluorescence NN cord-343256-n593kh7u 135 3 test test NN cord-343256-n593kh7u 135 4 for for IN cord-343256-n593kh7u 135 5 detection detection NN cord-343256-n593kh7u 135 6 of of IN cord-343256-n593kh7u 135 7 serum serum NN cord-343256-n593kh7u 135 8 antibody antibody NN cord-343256-n593kh7u 135 9 to to IN cord-343256-n593kh7u 135 10 rodent rodent NN cord-343256-n593kh7u 135 11 coronaviruses coronaviruse NNS cord-343256-n593kh7u 136 1 Two two CD cord-343256-n593kh7u 136 2 enzyme enzyme NN cord-343256-n593kh7u 136 3 immunoassays immunoassay NNS cord-343256-n593kh7u 136 4 for for IN cord-343256-n593kh7u 136 5 detection detection NN cord-343256-n593kh7u 136 6 of of IN cord-343256-n593kh7u 136 7 antibody antibody NN cord-343256-n593kh7u 136 8 to to IN cord-343256-n593kh7u 136 9 rodent rodent NN cord-343256-n593kh7u 136 10 coronaviruses coronaviruse NNS cord-343256-n593kh7u 136 11 Sialodacryoadenitis Sialodacryoadenitis NNP cord-343256-n593kh7u 136 12 virus virus NN cord-343256-n593kh7u 136 13 - - HYPH cord-343256-n593kh7u 136 14 associated associate VBN cord-343256-n593kh7u 136 15 lesions lesion NNS cord-343256-n593kh7u 136 16 in in IN cord-343256-n593kh7u 136 17 the the DT cord-343256-n593kh7u 136 18 lower low JJR cord-343256-n593kh7u 136 19 respiratory respiratory JJ cord-343256-n593kh7u 136 20 tract tract NN cord-343256-n593kh7u 136 21 of of IN cord-343256-n593kh7u 136 22 rats rat NNS cord-343256-n593kh7u 137 1 This this DT cord-343256-n593kh7u 137 2 work work NN cord-343256-n593kh7u 137 3 was be VBD cord-343256-n593kh7u 137 4 supported support VBN cord-343256-n593kh7u 137 5 by by IN cord-343256-n593kh7u 137 6 Natural Natural NNP cord-343256-n593kh7u 137 7 Sciences Sciences NNPS cord-343256-n593kh7u 137 8 Research Research NNP cord-343256-n593kh7u 137 9 Council Council NNP cord-343256-n593kh7u 137 10 grant grant NN cord-343256-n593kh7u 137 11 # # $ cord-343256-n593kh7u 137 12 A0071 A0071 NNP cord-343256-n593kh7u 137 13 , , , cord-343256-n593kh7u 137 14 the the DT cord-343256-n593kh7u 137 15 Ontario Ontario NNP cord-343256-n593kh7u 137 16 Ministry Ministry NNP cord-343256-n593kh7u 137 17 of of IN cord-343256-n593kh7u 137 18 Agriculture Agriculture NNP cord-343256-n593kh7u 137 19 and and CC cord-343256-n593kh7u 137 20 Food Food NNP cord-343256-n593kh7u 137 21 , , , cord-343256-n593kh7u 137 22 and and CC cord-343256-n593kh7u 137 23 USPHS USPHS NNP cord-343256-n593kh7u 137 24 grant grant VB cord-343256-n593kh7u 137 25 RR00393 rr00393 NN cord-343256-n593kh7u 137 26 . . . cord-343256-n593kh7u 138 1 The the DT cord-343256-n593kh7u 138 2 technical technical JJ cord-343256-n593kh7u 138 3 assistance assistance NN cord-343256-n593kh7u 138 4 of of IN cord-343256-n593kh7u 138 5 Ted Ted NNP cord-343256-n593kh7u 138 6 ] ] -RRB- cord-343256-n593kh7u 138 7 Eaton Eaton NNP cord-343256-n593kh7u 138 8 and and CC cord-343256-n593kh7u 138 9 Jean Jean NNP cord-343256-n593kh7u 138 10 Bagg Bagg NNP cord-343256-n593kh7u 138 11 are be VBP cord-343256-n593kh7u 138 12 gratefully gratefully RB cord-343256-n593kh7u 138 13 acknowledged acknowledge VBN cord-343256-n593kh7u 138 14 . . . cord-343256-n593kh7u 139 1 Special special JJ cord-343256-n593kh7u 139 2 thanks thank NNS cord-343256-n593kh7u 139 3 to to IN cord-343256-n593kh7u 139 4 Dr. Dr. NNP cord-343256-n593kh7u 139 5 Jeff Jeff NNP cord-343256-n593kh7u 139 6 Wilson Wilson NNP cord-343256-n593kh7u 139 7 , , , cord-343256-n593kh7u 139 8 Population Population NNP cord-343256-n593kh7u 139 9 Medicine Medicine NNP cord-343256-n593kh7u 139 10 , , , cord-343256-n593kh7u 139 11 University University NNP cord-343256-n593kh7u 139 12 of of IN cord-343256-n593kh7u 139 13 Guelph Guelph NNP cord-343256-n593kh7u 139 14 , , , cord-343256-n593kh7u 139 15 for for IN cord-343256-n593kh7u 139 16 the the DT cord-343256-n593kh7u 139 17 statistical statistical JJ cord-343256-n593kh7u 139 18 analyses analysis NNS cord-343256-n593kh7u 139 19 . . . cord-343256-n593kh7u 140 1 Received receive VBN cord-343256-n593kh7u 140 2 November November NNP cord-343256-n593kh7u 140 3 4 4 CD cord-343256-n593kh7u 140 4 , , , cord-343256-n593kh7u 140 5 1990 1990 CD cord-343256-n593kh7u 140 6 _SP