id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-102270-rfhtlodc	Azhar, Mohd.	Rapid, field-deployable nucleobase detection and identification using FnCas9	2020-04-21		.txt	text/plain	3971	217	50	We then fixed the position of this mutation with respect to PAM and changed every other base in the sgRNA sequence to identify which combination led to complete loss of cleavage of a wild type substrate in an in vitro cleavage (IVC) assay with FnCas9 ( Figure 1B, Supplementary Figure 1A ). Taken together, these experiments suggest that FELUDA design can be universally used for detection of SNVs and and would not require extensive optimization or validation steps for new SNVs. To aid users for quick design and implementation of FELUDA for a target SNV, we have developed a webtool JATAYU (Junction for Analysis and Target Design for Your FELUDA assay) that incorporates the above features and generates primer sequences for amplicon and sgRNA synthesis (https:// jatayu.igib.res.in, Supplementary Figure 2 ).	./cache/cord-102270-rfhtlodc.txt	./txt/cord-102270-rfhtlodc.txt