id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-102364-t5bt2eb4	Yao, Dehui	Human H-ferritin presenting RBM of spike glycoprotein as potential vaccine of SARS-CoV-2	2020-06-08		.txt	text/plain	1852	104	54	In an effort of utilizing human ferritin as nanoplatform for drug delivery, we engineered a fusion protein by presenting receptor-binding motif (RBM) of SARS-CoV-2 virus spike glycoprotein on the N-terminus of ferritin subunits. The designed fusion protein with a cage-like structure, similar to that of corona virus, is a potential anti-SARS-CoV-2 vaccine. We hereby show the construction, preparation, and characterization of the fusion protein RBM-HFtn. Our initial affinity study confirmed its biological activity towards ACE2 receptor which suggests its mode of action against SARS-CoV-2 could be either through vaccine therapy or blocking the cellular entry of virus as antagonist of ACE2 receptor. Antibodies targeting the spike glycoprotein of SARS-CoV and MERS-CoV, especially its receptor-binding domain (RBD), was found to efficiently neutralize virus infection [1, 2] . In this work, we engineered a human ferritin heavy chain (HFtn) by fusing and presenting the RBM of its spike glycoprotein as potential vaccine of SARS-CoV-2.	./cache/cord-102364-t5bt2eb4.txt	./txt/cord-102364-t5bt2eb4.txt