id author title date pages extension mime words sentences flesch summary cache txt cord-102412-cnlvyey4 Tekman, Mehmet A single-cell RNA-seq Training and Analysis Suite using the Galaxy Framework 2020-08-28 .txt text/plain 6419 300 53 Results Here we outline several Galaxy workflows and learning resources for scRNA-seq, with the aim of providing a comprehensive analysis environment paired with a thorough user learning experience that bridges the knowledge gap between the computational methods and the underlying cell biology. The analysis of scRNA-seq within Galaxy was a two-pronged e ort concentrated on bringing high quality single-cell tools into Galaxy, and providing the necessary work ows and training to accompany them. The training pictographically guides users through the concepts of extracting cell barcodes from the protocol, explains the signi cance of UMIs in the process of read deduplication with illustrative examples, and instructs the user in the process of performing further quality controls on their data during the post-mapping process via RNA STAR and other tools that are native to Galaxy. A Galaxy-based training resource for single-cell RNA-sequencing quality control and analyses ./cache/cord-102412-cnlvyey4.txt ./txt/cord-102412-cnlvyey4.txt