id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-102551-8igfuaw2	Boonyaratanakornkit, Jim	Protective Antibodies Against Human Parainfluenza Virus Type 3 (HPIV3) Infection	2020-10-30		.txt	text/plain	8067	462	53	HPIV3, like respiratory syncytial virus (RSV), infects early in life and frequently causes severe bronchiolitis and pneumonia in infants under six months of age who are unable to mount a robust antibody response 2,3 . HPIV3 F was recently stabilized in the preF conformation and induced higher serum neutralizing titers than the HPIV3 postF To focus upon B cells producing neutralizing antibodies, we modified our assay to sort individual B cells onto irradiated 3T3 feeder cells expressing CD40L, IL-2, and IL-21 to allow for higher throughput screening of culture supernatants for neutralization prior to antibody cloning, as described 27 . Using this approach, we found that 14% of IgD -HPIV3 preF-binding B cells sorted from tonsils produced HPIV3 neutralizing antibodies, as compared to 5% from the spleen and 2% from peripheral blood (Fig. 3b) .	./cache/cord-102551-8igfuaw2.txt	./txt/cord-102551-8igfuaw2.txt