id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-102908-sr7j8z9c	Mersmann, Sophia F.	Learning to count: determining the stoichiometry of bio-molecular complexes using fluorescence microscopy and statistical modelling	2020-07-24		.txt	text/plain	5244	260	42	We used differential binary labelling and statistical modelling to extract estimates of stoichiometry, our strategy is outlined in Figure 1 ; note that this approach can be generalised to apply to many other multi-component systems (i.e. how many protein x are found in assembly y?). As described above, our experimental design utilizes antibody labelled with spectrally distinct dyes allowing binary scoring of individual virus particles as positive if they interact with at least one Ab B molecule ( Figure 1 ). We have demonstrated quantitative analysis of 9C12 interaction with individual Adv particles ( Figure 3) ; we have confirmed that differential labelling of antibody does not bias binding ( Figure 4A & B) ; and that we could detect single molecules of 9C12 Biotin allowing discrimination of positive and negative AdV-9C12 complexes ( Figure 4C & D). However, using stoichiometric estimates to calibrate fluorescent data revealed population heterogeneity with a small proportion of virus particles binding ∼200 antibody molecules.	./cache/cord-102908-sr7j8z9c.txt	./txt/cord-102908-sr7j8z9c.txt