id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-102976-cic1gxrk	Patel, Roosheel S.	Single cell resolution landscape of equine peripheral blood mononuclear cells reveals diverse immune cell subtypes including T-bet+ B cells	2020-05-07		.txt	text/plain	7155	378	45	Single cell RNA-Sequencing (scRNA-Seq) offers an alternative to flow cytometry as it defines different cell types (and their functional states) by gene expression patterns rather than surface marker expression. To characterize equine PBMC at high resolution and establish a corresponding peripheral blood immune cell atlas, we independently analyzed scRNA-Seq data for the constituent clusters of each major cell group, except Basophils due to the low number of cells. Additional genes with significantly elevated expression levels in cluster 28 include NR4A1 (transcription factor necessary for differentiation of non-classical monocytes in mice) (23) , CX3CR1 (chemokine receptor characteristic of nonclassical monocytes in humans and mice) (24, 25) , and HES4 (target of NOTCH signaling implicated in non-classical monocyte generation) (26) (Fig. 2E ). To further support our cell type annotations and assess potential differences in monocyte/DC subsets between horses and humans, we performed cross-species hierarchical clustering with a human PBMC public reference scRNA-Seq data set ( Fig. S2A-B, Fig. 2G ).	./cache/cord-102976-cic1gxrk.txt	./txt/cord-102976-cic1gxrk.txt