id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-103015-3dxwbmd2	Shengjuler, Djoshkun	The RNA-binding site of poliovirus 3C protein doubles as a phosphoinositide-binding domain	2017-08-04		.txt	text/plain	7069	411	63	Molecular-docking simulations revealed a putative site of PIP binding to poliovirus (PV) 3C protein that was validated using NMR spectroscopy. 93 Validation of PIP-binding sites by NMR 94 In order to test the validity of our docking observations, we titrated 15 N-labeled 3C protein 95 with soluble dibutyl-PI4P to observe potential NMR chemical shift perturbations (CSPs), which 96 would indicate chemical environment changes in the presence of PI4P (Figure 2) . Out of the three 97 basic residues of the major cluster that were predicted to interact with the PI4P, R13 showed the 98 largest CSP (Figure 2A ). Titration of PI4P into a solution containing 3C caused CSPs that were consistent 249 with the major PI4P-binding site observed computationally (Figure 2A) .	./cache/cord-103015-3dxwbmd2.txt	./txt/cord-103015-3dxwbmd2.txt